Standard name | |
---|---|
Human Ortholog | |
Description | Nuclear envelope protein; anchored to the nuclear inner membrane, that interacts with the vacuolar membrane protein Vac8p to promote formation of nucleus-vacuole junctions during piecemeal microautophagy of the nucleus (PMN) |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud | 1 | 0 | 2 | 0 | 6 | 8 | 16 | 9 | 20 | 9 | 30 | 21 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 3 | 1 | 0 | 5 | 10 |
Bud Neck | 0 | 0 | 0 | 0 | 2 | 5 | 4 | 5 | 4 | 2 | 5 | 8 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 2 | 10 | 7 | 2 | 2 | 6 | 8 | 10 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 8 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 8 | 4 | 9 | 15 | 41 | 25 | 25 | 64 | 23 | 21 | 11 | 17 | 1 | 20 | 37 | 11 | 11 | 7 | 4 | 2 | 0 | 0 | 1 | 3 |
Endoplasmic Reticulum | 0 | 1 | 1 | 1 | 0 | 2 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 1 | 1 | 2 | 1 | 1 | 0 | 2 | 5 | 1 | 3 | 7 |
Endosome | 23 | 53 | 28 | 102 | 152 | 67 | 151 | 301 | 134 | 266 | 86 | 140 | 0 | 23 | 34 | 56 | 55 | 43 | 18 | 48 | 22 | 3 | 21 | 18 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 |
Mitochondria | 11 | 10 | 10 | 16 | 70 | 152 | 183 | 47 | 120 | 99 | 133 | 139 | 0 | 2 | 0 | 12 | 8 | 7 | 1 | 4 | 3 | 0 | 5 | 8 |
Nucleus | 2 | 1 | 3 | 3 | 23 | 39 | 81 | 81 | 40 | 37 | 25 | 48 | 0 | 3 | 1 | 1 | 5 | 2 | 0 | 2 | 0 | 0 | 2 | 10 |
Nuclear Periphery | 1 | 7 | 1 | 6 | 6 | 15 | 46 | 28 | 23 | 31 | 15 | 21 | 0 | 0 | 1 | 3 | 0 | 1 | 8 | 20 | 11 | 11 | 82 | 88 |
Nucleolus | 90 | 186 | 124 | 249 | 448 | 518 | 628 | 483 | 576 | 757 | 589 | 736 | 13 | 92 | 141 | 123 | 117 | 89 | 4 | 16 | 3 | 0 | 3 | 5 |
Peroxisomes | 12 | 1 | 12 | 28 | 47 | 9 | 30 | 52 | 37 | 51 | 18 | 36 | 0 | 3 | 5 | 12 | 6 | 6 | 0 | 0 | 0 | 0 | 0 | 1 |
SpindlePole | 97 | 175 | 121 | 271 | 484 | 379 | 466 | 445 | 468 | 651 | 434 | 612 | 15 | 159 | 173 | 120 | 87 | 76 | 99 | 174 | 124 | 6 | 15 | 16 |
Vac/Vac Membrane | 6 | 29 | 10 | 12 | 64 | 61 | 52 | 83 | 38 | 38 | 11 | 15 | 0 | 2 | 7 | 17 | 15 | 6 | 0 | 3 | 2 | 0 | 19 | 46 |
Unique Cell Count | 145 | 270 | 174 | 368 | 819 | 827 | 1069 | 996 | 883 | 1130 | 806 | 1104 | 17 | 192 | 253 | 184 | 164 | 118 | 145 | 291 | 184 | 23 | 163 | 223 |
Labelled Cell Count | 252 | 468 | 321 | 703 | 1345 | 1290 | 1690 | 1601 | 1485 | 1970 | 1366 | 1804 | 29 | 307 | 409 | 360 | 305 | 238 | 145 | 291 | 184 | 23 | 163 | 223 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.6 | 8.1 | 6.4 | 6.4 | 6.2 | 5.6 | 5.2 | 6.4 | 5.7 | 6.0 | 5.7 | 5.8 | 6.1 | 7.5 | 7.9 | 8.7 | 8.4 | 9.0 | – | 8.6 | 8.8 |
Std Deviation (1e-4) | 1.4 | 1.6 | 1.2 | 1.4 | 1.6 | 1.3 | 1.3 | 1.6 | 1.4 | 1.5 | 1.5 | 1.6 | 1.1 | 1.3 | 1.6 | 1.7 | 1.5 | 1.5 | – | 1.6 | 2.0 |
Intensity Change (Log2) | – | – | – | -0.01 | -0.05 | -0.21 | -0.32 | -0.01 | -0.17 | -0.1 | -0.17 | -0.15 | -0.06 | 0.22 | 0.3 | 0.43 | 0.39 | 0.49 | – | 0.42 | 0.45 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -0.6 | -0.1 | -1.4 | 0 | 0.6 | -1.8 | 0 | 0 | 0 | 0 | 1.9 | 3.1 | 0.3 | 0.6 | 0.3 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 3.0 | 0.8 | -3.3 | -0.7 | 3.8 | -0.3 | 2.2 | -2.0 | -1.2 | 0 | -1.1 | -0.8 | 3.2 | 3.7 | 4.0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | -0.7 | 1.2 | 4.1 | 3.8 | -0.6 | 2.9 | 1.3 | 3.6 | 2.6 | 0 | -2.5 | 0 | 0.3 | -0.4 | 0.1 |
Nucleus | 0 | 0 | 1.8 | 2.9 | 3.0 | 1.7 | 1.1 | 0 | 1.6 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 2.4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | -0.8 | -4.0 | -2.2 | -3.1 | -5.5 | -1.5 | -1.1 | 0.5 | -1.2 | 0.5 | -4.5 | -3.3 | -0.9 | 0 | 0.8 |
Peroxisomes | 0.3 | -0.6 | 0 | -2.8 | -0.9 | -1.6 | -1.4 | -3.2 | -2.3 | 0 | -2.6 | -2.6 | -0.1 | -1.3 | -0.6 |
SpindlePole | 1.0 | -2.6 | -5.7 | -6.4 | -6.1 | -4.0 | -3.0 | -3.8 | -3.5 | 1.6 | 3.0 | -0.3 | -0.9 | -3.1 | -0.9 |
Vacuole | -1.4 | 0.9 | 0.8 | -0.5 | 1.2 | -0.8 | -1.6 | 0 | 0 | 0 | -2.5 | -1.5 | 1.3 | 1.2 | -0.2 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.2088 | 4.4966 | 3.7335 | 3.2814 | 3.1266 | 3.8452 | 3.4838 | 5.5642 | 3.8568 | 3.8665 | 2.805 | 3.7707 | 2.4454 | 4.0979 | 3.1398 | 3.2177 | 2.3804 | 3.3601 |
Actin | 0.0046 | 0.0001 | 0.0008 | 0.0012 | 0.0002 | 0 | 0.0013 | 0 | 0.0092 | 0.0018 | 0.0135 | 0.0022 | 0.0015 | 0 | 0.0001 | 0.0005 | 0.0001 | 0 |
Bud | 0.0002 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0.0009 | 0.0025 | 0.0009 | 0.0014 | 0.0001 | 0.0017 | 0.0002 | 0.001 | 0.0002 | 0.0009 | 0.0002 | 0.0003 | 0.001 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0006 |
Bud Periphery | 0.0002 | 0 | 0.0001 | 0.0004 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0002 | 0.0001 | 0.0002 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0.0007 | 0.0005 | 0.0004 | 0.0005 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0004 | 0.0002 | 0.003 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 |
Cell Periphery | 0.0002 | 0.0001 | 0.0002 | 0.0004 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.0057 | 0.0026 | 0.0002 | 0.0021 | 0.0015 | 0.0001 | 0.001 | 0 | 0.0001 | 0.0011 | 0.0012 | 0.0051 | 0.0002 | 0 | 0.0001 | 0.0004 | 0.0001 | 0 |
Cytoplasmic Foci | 0.092 | 0.0784 | 0.0458 | 0.0554 | 0.0314 | 0.0389 | 0.0663 | 0.0828 | 0.056 | 0.0924 | 0.0391 | 0.0463 | 0.0973 | 0.0771 | 0.0578 | 0.0708 | 0.0621 | 0.0643 |
Eisosomes | 0.0001 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0024 | 0.0016 | 0.0002 | 0.0021 | 0.0007 | 0.0001 | 0.0001 | 0 | 0 | 0.0003 | 0.0016 | 0.0002 | 0.0008 | 0 | 0.0001 | 0.0023 | 0.0001 | 0 |
Endosome | 0.0129 | 0.0027 | 0.0104 | 0.0108 | 0.0052 | 0.0012 | 0.0034 | 0.0007 | 0.0008 | 0.0043 | 0.0084 | 0.0037 | 0.0049 | 0.0007 | 0.0009 | 0.0105 | 0.0025 | 0.0008 |
Golgi | 0.0052 | 0.0015 | 0.0067 | 0.0055 | 0.0037 | 0.0019 | 0.0035 | 0.0019 | 0.0018 | 0.0036 | 0.0046 | 0.0048 | 0.006 | 0.0023 | 0.0021 | 0.0036 | 0.0051 | 0.0025 |
Lipid Particles | 0.1459 | 0.1136 | 0.1026 | 0.1419 | 0.0944 | 0.1154 | 0.1812 | 0.1699 | 0.1322 | 0.1334 | 0.0796 | 0.181 | 0.206 | 0.1877 | 0.1427 | 0.1802 | 0.2795 | 0.2102 |
Mitochondria | 0.0101 | 0.0005 | 0.0042 | 0.0056 | 0.0033 | 0.0004 | 0.0001 | 0 | 0.0005 | 0.0001 | 0.0024 | 0.0101 | 0.0035 | 0 | 0.0001 | 0.0003 | 0 | 0 |
None | 0.0047 | 0.0002 | 0.0002 | 0.0018 | 0.012 | 0.0001 | 0.0021 | 0 | 0.0001 | 0.0047 | 0.001 | 0.0041 | 0.0004 | 0 | 0.0001 | 0.0002 | 0.0001 | 0 |
Nuclear Periphery | 0.0146 | 0.0102 | 0.0192 | 0.0638 | 0.0493 | 0.0044 | 0.0082 | 0.0016 | 0.0076 | 0.006 | 0.1193 | 0.012 | 0.0127 | 0.0036 | 0.0094 | 0.0501 | 0.0404 | 0.003 |
Nucleolus | 0.118 | 0.2014 | 0.1488 | 0.1611 | 0.042 | 0.1705 | 0.052 | 0.1467 | 0.0543 | 0.0495 | 0.0154 | 0.0457 | 0.1264 | 0.1477 | 0.1292 | 0.0717 | 0.0271 | 0.0923 |
Nucleus | 0.0131 | 0.0269 | 0.0158 | 0.0334 | 0.0058 | 0.0122 | 0.0056 | 0.0052 | 0.0109 | 0.0045 | 0.0022 | 0.0022 | 0.0058 | 0.0053 | 0.0055 | 0.004 | 0.0018 | 0.0045 |
Peroxisomes | 0.0132 | 0.0008 | 0.002 | 0.0042 | 0.0175 | 0.0006 | 0.0022 | 0.0005 | 0.0016 | 0.0032 | 0.0075 | 0.0036 | 0.0028 | 0.0006 | 0.0007 | 0.0006 | 0.0009 | 0.0004 |
Punctate Nuclear | 0.5523 | 0.5556 | 0.6375 | 0.4971 | 0.731 | 0.6514 | 0.6722 | 0.5892 | 0.7243 | 0.6935 | 0.6998 | 0.6776 | 0.5256 | 0.5745 | 0.6507 | 0.6027 | 0.5788 | 0.6211 |
Vacuole | 0.0015 | 0.0005 | 0.0031 | 0.0065 | 0.0007 | 0.0003 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0003 | 0.0003 | 0.0008 | 0.0001 | 0.0001 | 0.0006 | 0.0001 | 0.0001 |
Vacuole Periphery | 0.0014 | 0.0004 | 0.001 | 0.0048 | 0.0011 | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0031 | 0.0003 | 0.001 | 0.0001 | 0.0003 | 0.0013 | 0.0009 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 11.9935 | 6.1266 | 9.85 | 14.4695 | 17.7716 | 13.6993 | 13.9378 | 16.0268 | 20.9147 | 13.5422 |
Translational Efficiency | 0.7005 | 1.0965 | 0.8227 | 0.9006 | 0.5117 | 0.8289 | 0.7372 | 0.762 | 0.6315 | 0.6536 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1644 | 1436 | 2716 | 1538 | 1620 | 877 | 2359 | 1586 | 3264 | 2313 | 5075 | 3124 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 754.48 | 849.17 | 1299.23 | 1008.98 | 804.12 | 769.92 | 925.80 | 952.64 | 779.12 | 819.12 | 1125.65 | 980.38 |
Standard Deviation | 110.54 | 143.08 | 196.66 | 177.72 | 119.72 | 117.16 | 125.28 | 160.05 | 117.83 | 139.26 | 250.37 | 171.31 |
Intensity Change Log 2 | — | 0.170571 | 0.784102 | 0.419343 | — | -0.062702 | 0.203290 | 0.244520 | — | 0.054932 | 0.513574 | 0.331795 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000113 | 0.000195 | 0.000161 | 0.000224 | 0.000063 | 0.000297 | 0.000058 | 0.000211 | 0.000088 | 0.000233 | 0.000113 | 0.000217 |
Bud Neck | 0.002416 | 0.003425 | 0.004641 | 0.010106 | 0.002071 | 0.002268 | 0.004752 | 0.007832 | 0.002245 | 0.002987 | 0.004692 | 0.008951 |
Bud Site | 0.001041 | 0.001232 | 0.000902 | 0.007040 | 0.001082 | 0.001292 | 0.002231 | 0.005652 | 0.001061 | 0.001255 | 0.001520 | 0.006335 |
Cell Periphery | 0.000111 | 0.000140 | 0.000053 | 0.000094 | 0.000074 | 0.000122 | 0.000049 | 0.000101 | 0.000093 | 0.000133 | 0.000051 | 0.000097 |
Cytoplasm | 0.002613 | 0.001226 | 0.000310 | 0.012545 | 0.001638 | 0.001399 | 0.006359 | 0.022745 | 0.002129 | 0.001291 | 0.003122 | 0.017723 |
Cytoplasmic Foci | 0.020644 | 0.024705 | 0.019214 | 0.026566 | 0.018292 | 0.022611 | 0.014981 | 0.045255 | 0.019477 | 0.023911 | 0.017246 | 0.036054 |
Eisosomes | 0.000129 | 0.000215 | 0.000294 | 0.000030 | 0.000165 | 0.000094 | 0.000024 | 0.000027 | 0.000147 | 0.000169 | 0.000169 | 0.000029 |
Endoplasmic Reticulum | 0.002279 | 0.002685 | 0.000908 | 0.001931 | 0.001241 | 0.003336 | 0.000785 | 0.002447 | 0.001764 | 0.002931 | 0.000851 | 0.002193 |
Endosome | 0.011269 | 0.011948 | 0.001557 | 0.025408 | 0.007307 | 0.013721 | 0.013181 | 0.044094 | 0.009302 | 0.012620 | 0.006960 | 0.034894 |
Golgi | 0.002112 | 0.002763 | 0.000903 | 0.002929 | 0.001320 | 0.003550 | 0.000483 | 0.002353 | 0.001719 | 0.003061 | 0.000708 | 0.002637 |
Lipid Particles | 0.027186 | 0.022772 | 0.009176 | 0.047110 | 0.027567 | 0.053710 | 0.009730 | 0.067708 | 0.027375 | 0.034503 | 0.009433 | 0.057567 |
Mitochondria | 0.000898 | 0.001312 | 0.000841 | 0.001547 | 0.000808 | 0.002743 | 0.000329 | 0.001262 | 0.000853 | 0.001855 | 0.000603 | 0.001402 |
Mitotic Spindle | 0.015539 | 0.039897 | 0.182110* | 0.393605* | 0.023315 | 0.033384 | 0.215437* | 0.281123* | 0.019398 | 0.037428 | 0.197601* | 0.336500* |
None | 0.001318 | 0.000938 | 0.000265 | 0.000708 | 0.000782 | 0.001468 | 0.000448 | 0.000777 | 0.001052 | 0.001139 | 0.000350 | 0.000743 |
Nuclear Periphery | 0.006518 | 0.006467 | 0.021011 | 0.028796 | 0.004980 | 0.008193 | 0.012710 | 0.017648 | 0.005754 | 0.007121 | 0.017153 | 0.023137 |
Nuclear Periphery Foci | 0.835728* | 0.754665* | 0.307827* | 0.385426* | 0.836205* | 0.767589* | 0.674856* | 0.454824* | 0.835964* | 0.759565* | 0.478432* | 0.420658* |
Nucleolus | 0.050449 | 0.086470 | 0.375557* | 0.023867 | 0.054320 | 0.060145 | 0.028773 | 0.015475 | 0.052370 | 0.076488 | 0.214362 | 0.019606 |
Nucleus | 0.010057 | 0.020203 | 0.045975 | 0.012892 | 0.009185 | 0.013443 | 0.010052 | 0.012125 | 0.009624 | 0.017640 | 0.029277 | 0.012503 |
Peroxisomes | 0.007589 | 0.014918 | 0.025015 | 0.002854 | 0.007695 | 0.008204 | 0.001663 | 0.003739 | 0.007641 | 0.012372 | 0.014161 | 0.003303 |
Vacuole | 0.001752 | 0.003176 | 0.002550 | 0.013603 | 0.001692 | 0.002003 | 0.002906 | 0.012956 | 0.001722 | 0.002731 | 0.002715 | 0.013275 |
Vacuole Periphery | 0.000243 | 0.000649 | 0.000731 | 0.002721 | 0.000200 | 0.000429 | 0.000193 | 0.001648 | 0.000222 | 0.000566 | 0.000481 | 0.002176 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -2.50 | -2.41 | -4.36 | -1.91 | -3.46 | -3.99 | -0.75 | -4.34 | 0.73 | -4.17 | -4.96 | -2.75 | -6.08 | -0.65 | -5.26 |
Bud Neck | -2.40 | -6.64 | -11.53 | -10.13 | -8.67 | -0.55 | -7.15 | -10.60 | -10.18 | -4.34 | -2.60 | -9.83 | -15.60 | -13.81 | -9.42 |
Bud Site | -0.96 | 0.31 | -7.70 | -7.50 | -7.81 | -0.17 | -4.64 | -6.23 | -5.77 | -3.76 | -0.60 | -4.09 | -9.90 | -9.57 | -8.46 |
Cell Periphery | -0.78 | 2.78 | 0.32 | 1.29 | -7.47 | -2.69 | 2.15 | -2.61 | 0.91 | -6.07 | -1.89 | 3.42 | -0.92 | 1.53 | -9.47 |
Cytoplasm | 1.92 | 3.28 | -8.01 | -8.85 | -9.23 | 0.61 | -4.77 | -9.99 | -10.19 | -7.36 | 1.98 | -2.35 | -12.79 | -13.52 | -11.72 |
Cytoplasmic Foci | -1.34 | 0.79 | -2.91 | -1.56 | -4.22 | -1.43 | 0.83 | -9.47 | -6.98 | -10.47 | -2.07 | 1.11 | -9.02 | -6.48 | -10.84 |
Eisosomes | -4.56 | -11.19 | 8.39 | 12.97 | 30.83* | 5.62 | 11.95 | 11.46 | 11.42 | -2.44 | -1.61 | -1.78 | 14.02 | 15.26 | 27.51 |
Endoplasmic Reticulum | -0.84 | 2.94 | 0.15 | 1.26 | -3.71 | -2.95 | 2.70 | -2.88 | 0.92 | -3.85 | -3.06 | 3.62 | -1.68 | 1.36 | -5.30 |
Endosome | -0.53 | 7.99 | -7.76 | -7.44 | -15.89 | -3.92 | -6.42 | -16.53 | -11.81 | -13.40 | -3.06 | 2.21 | -17.55 | -14.24 | -20.14 |
Golgi | -1.33 | 5.01 | -0.68 | 0.22 | -3.02 | -5.14 | 9.20 | -1.61 | 2.34 | -3.38 | -4.30 | 7.59 | -1.50 | 1.56 | -4.45 |
Lipid Particles | 1.72 | 8.45 | -3.90 | -5.42 | -10.38 | -5.91 | 7.74 | -8.34 | -1.30 | -14.45 | -3.14 | 11.45 | -8.82 | -5.63 | -17.71 |
Mitochondria | -1.06 | 0.20 | -1.95 | -0.72 | -2.59 | -1.67 | 0.73 | -0.96 | 1.33 | -4.86 | -1.93 | 0.70 | -1.88 | 0.80 | -4.71 |
Mitotic Spindle | -5.71 | -29.69* | -37.39* | -33.45* | -18.40 | -1.41 | -28.91* | -28.28* | -25.85* | -4.05 | -5.08 | -41.08 | -46.20 | -41.58* | -15.97 |
None | 1.37 | 4.26 | 0.29 | -1.11 | -4.27 | -1.13 | 0.89 | -0.50 | 1.00 | -3.49 | -0.16 | 3.31 | -0.14 | 0.04 | -5.58 |
Nuclear Periphery | -0.05 | -11.31 | -9.73 | -10.52 | -3.02 | -1.59 | -5.34 | -7.54 | -4.26 | -3.33 | -1.31 | -12.03 | -12.26 | -10.87 | -4.17 |
Nuclear Periphery Foci | 7.82 | 60.49* | 43.35* | 32.61* | -5.00 | 5.37 | 19.25 | 35.73* | 24.33* | 17.72 | 9.53 | 53.29 | 55.58 | 39.98* | 8.01 |
Nucleolus | -6.79 | -48.92* | 5.99 | 12.02 | 54.99* | -0.83 | 5.36 | 9.34 | 7.93 | 5.73 | -5.91 | -34.88* | 10.89 | 14.93 | 44.91* |
Nucleus | -5.15 | -19.49 | -4.90 | -0.43 | 9.49 | -2.29 | -1.81 | -3.67 | -1.25 | -2.09 | -5.71 | -16.35 | -6.10 | -0.62 | 6.87 |
Peroxisomes | -5.07 | -17.25 | 5.69 | 9.06 | 27.93* | -0.52 | 8.62 | 4.76 | 4.75 | -5.80 | -4.85 | -9.93 | 7.37 | 10.02 | 21.61* |
Vacuole | -5.08 | -4.56 | -10.31 | -9.58 | -9.84 | -1.47 | -3.69 | -9.55 | -9.38 | -8.11 | -5.32 | -5.11 | -14.03 | -13.29 | -12.72 |
Vacuole Periphery | -2.58 | -7.50 | -4.64 | -3.63 | -3.57 | -2.91 | -0.39 | -3.97 | -3.24 | -3.95 | -3.38 | -6.59 | -6.09 | -4.67 | -5.11 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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