Standard name | |
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Human Ortholog | |
Description | Protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 2 | 1 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 8 | 9 | 0 | 2 | 4 | 5 | 1 | 2 | 2 | 2 | 5 | 20 |
Bud | 4 | 2 | 0 | 8 | 11 | 10 | 28 | 34 | 27 | 32 | 19 | 18 | 3 | 6 | 1 | 6 | 1 | 2 | 1 | 0 | 0 | 0 | 4 | 5 |
Bud Neck | 0 | 0 | 0 | 1 | 4 | 3 | 4 | 13 | 7 | 11 | 4 | 5 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 5 | 9 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 2 | 6 | 6 | 5 | 5 | 4 | 8 | 0 | 0 | 0 | 3 | 0 | 2 | – | – | – | – | – | – |
Cell Periphery | 0 | 1 | 0 | 5 | 3 | 4 | 1 | 1 | 1 | 1 | 0 | 2 | 7 | 9 | 9 | 4 | 2 | 4 | 0 | 0 | 0 | 0 | 0 | 1 |
Cytoplasm | 30 | 14 | 1 | 25 | 54 | 60 | 79 | 120 | 93 | 136 | 92 | 113 | 37 | 47 | 55 | 2 | 4 | 1 | 19 | 3 | 0 | 4 | 8 | 6 |
Endoplasmic Reticulum | 3 | 7 | 0 | 2 | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 9 | 9 | 4 | 2 | 3 | 3 | 0 | 0 | 0 | 0 | 2 | 10 |
Endosome | 0 | 3 | 0 | 6 | 30 | 14 | 4 | 5 | 1 | 5 | 1 | 3 | 9 | 46 | 75 | 4 | 1 | 0 | 10 | 6 | 7 | 13 | 30 | 32 |
Golgi | 1 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 8 | 20 | 19 | 22 | 22 | 13 | 5 | 4 | 2 | 1 | 5 | 6 |
Mitochondria | 144 | 111 | 14 | 151 | 223 | 228 | 360 | 363 | 278 | 301 | 236 | 216 | 71 | 100 | 123 | 6 | 1 | 3 | 116 | 86 | 56 | 137 | 267 | 324 |
Nucleus | 1 | 1 | 0 | 3 | 5 | 8 | 20 | 22 | 18 | 9 | 7 | 5 | 4 | 8 | 5 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 3 | 2 |
Nuclear Periphery | 2 | 0 | 1 | 0 | 0 | 0 | 2 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 2 | 0 | 2 | 0 | 0 | 0 | 2 | 2 | 6 |
Nucleolus | 2 | 1 | 0 | 1 | 0 | 0 | 4 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 2 | 2 | 3 | 0 | 0 | 0 | 2 | 7 | 7 |
Peroxisomes | 0 | 0 | 0 | 9 | 17 | 9 | 7 | 2 | 2 | 2 | 0 | 3 | 0 | 7 | 29 | 4 | 2 | 1 | 6 | 1 | 5 | 4 | 8 | 14 |
SpindlePole | 2 | 0 | 1 | 16 | 37 | 32 | 62 | 67 | 28 | 35 | 10 | 36 | 7 | 15 | 9 | 1 | 0 | 0 | 1 | 0 | 1 | 2 | 13 | 11 |
Vac/Vac Membrane | 11 | 15 | 0 | 6 | 50 | 26 | 17 | 12 | 10 | 14 | 10 | 8 | 26 | 56 | 62 | 11 | 6 | 9 | 4 | 28 | 0 | 8 | 30 | 29 |
Unique Cell Count | 174 | 127 | 17 | 202 | 340 | 324 | 470 | 513 | 379 | 437 | 315 | 331 | 162 | 266 | 310 | 61 | 41 | 40 | 174 | 138 | 79 | 189 | 408 | 500 |
Labelled Cell Count | 200 | 157 | 18 | 236 | 437 | 396 | 595 | 647 | 472 | 552 | 385 | 418 | 190 | 333 | 393 | 71 | 49 | 48 | 174 | 138 | 79 | 189 | 408 | 500 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.8 | 4.8 | 4.3 | 5.1 | 5.0 | 4.1 | 3.8 | 4.1 | 3.6 | 3.6 | 3.3 | 3.5 | 6.0 | 6.5 | 6.0 | 9.6 | 10.9 | 10.7 | 5.0 | 5.4 | 6.0 |
Std Deviation (1e-4) | 0.7 | 0.7 | 0.7 | 1.5 | 1.2 | 1.4 | 1.5 | 1.2 | 1.5 | 1.3 | 1.3 | 1.1 | 1.0 | 1.8 | 1.1 | 2.1 | 2.7 | 2.2 | 0.8 | 1.4 | 1.7 |
Intensity Change (Log2) | – | – | – | 0.24 | 0.22 | -0.06 | -0.17 | -0.08 | -0.27 | -0.25 | -0.38 | -0.28 | 0.48 | 0.59 | 0.48 | 1.15 | 1.34 | 1.32 | 0.23 | 0.33 | 0.49 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.8486 | 1.6868 | 1.4857 | 1.4037 | 1.0841 | 1.6836 | 1.5029 | 2.3305 | 1.7654 | 1.6127 | 1.3375 | 1.7651 | 0.792 | 1.1895 | 0.7894 | 0.529 | 0.3806 | 0.9899 |
Actin | 0.0195 | 0.003 | 0.0039 | 0.0153 | 0.0017 | 0.0049 | 0.0247 | 0.0019 | 0.003 | 0.0112 | 0.0056 | 0.0013 | 0.007 | 0.0125 | 0.0029 | 0.0015 | 0.0014 | 0.0011 |
Bud | 0.0006 | 0.0037 | 0.0018 | 0.0085 | 0.0025 | 0.0002 | 0.0007 | 0.0019 | 0.0015 | 0.0017 | 0.0003 | 0.0004 | 0.0037 | 0.0058 | 0.0011 | 0.0041 | 0.0041 | 0.0009 |
Bud Neck | 0.0017 | 0.0006 | 0.0007 | 0.0012 | 0.0007 | 0.0015 | 0.0013 | 0.0005 | 0.0005 | 0.0008 | 0.0003 | 0.0021 | 0.0003 | 0.0006 | 0.0003 | 0.0011 | 0.0023 | 0.0006 |
Bud Periphery | 0.0016 | 0.0041 | 0.0053 | 0.0343 | 0.0108 | 0.0006 | 0.0016 | 0.0052 | 0.003 | 0.0082 | 0.0008 | 0.0012 | 0.0039 | 0.0039 | 0.003 | 0.0149 | 0.0072 | 0.0008 |
Bud Site | 0.004 | 0.0132 | 0.0022 | 0.0024 | 0.0021 | 0.0004 | 0.0042 | 0.01 | 0.0046 | 0.0021 | 0.0007 | 0.0003 | 0.0041 | 0.0184 | 0.0052 | 0.0012 | 0.0024 | 0.0003 |
Cell Periphery | 0.0007 | 0.0008 | 0.0004 | 0.0005 | 0.0009 | 0.0002 | 0.0005 | 0.0013 | 0.0006 | 0.0006 | 0.0001 | 0.0002 | 0.0013 | 0.0036 | 0.0031 | 0.0004 | 0.0005 | 0.0002 |
Cytoplasm | 0.0076 | 0.0059 | 0.0033 | 0.0198 | 0.002 | 0.0088 | 0.0079 | 0.0035 | 0.0054 | 0.0029 | 0.0029 | 0.0033 | 0.0051 | 0.0047 | 0.0052 | 0.0008 | 0.0057 | 0.0045 |
Cytoplasmic Foci | 0.0217 | 0.0232 | 0.0182 | 0.0276 | 0.0274 | 0.0115 | 0.0415 | 0.0375 | 0.0216 | 0.0098 | 0.0382 | 0.0234 | 0.0157 | 0.0354 | 0.0195 | 0.0139 | 0.0253 | 0.0158 |
Eisosomes | 0.0007 | 0.0002 | 0.0002 | 0.0001 | 0.0002 | 0.0002 | 0.0005 | 0.0003 | 0.0002 | 0.0004 | 0.0001 | 0.0001 | 0.0004 | 0.001 | 0.0004 | 0.0001 | 0.0001 | 0.0001 |
Endoplasmic Reticulum | 0.0037 | 0.0035 | 0.0011 | 0.001 | 0.0009 | 0.0011 | 0.0025 | 0.0006 | 0.0011 | 0.0019 | 0.0032 | 0.0005 | 0.0013 | 0.0006 | 0.0007 | 0.0002 | 0.0003 | 0.0004 |
Endosome | 0.0897 | 0.089 | 0.0846 | 0.073 | 0.0652 | 0.0442 | 0.0619 | 0.0795 | 0.056 | 0.0575 | 0.1057 | 0.0355 | 0.066 | 0.0979 | 0.0566 | 0.0413 | 0.1222 | 0.045 |
Golgi | 0.0353 | 0.043 | 0.0288 | 0.0473 | 0.0426 | 0.0329 | 0.0353 | 0.0526 | 0.0197 | 0.0225 | 0.0329 | 0.0272 | 0.0435 | 0.0772 | 0.0301 | 0.0312 | 0.0425 | 0.0395 |
Lipid Particles | 0.0544 | 0.0109 | 0.0149 | 0.0274 | 0.032 | 0.0291 | 0.0444 | 0.0265 | 0.0112 | 0.0137 | 0.0226 | 0.0186 | 0.0199 | 0.022 | 0.0099 | 0.0131 | 0.0071 | 0.0094 |
Mitochondria | 0.6873 | 0.7402 | 0.7683 | 0.7004 | 0.7148 | 0.8163 | 0.6797 | 0.6925 | 0.8218 | 0.818 | 0.7181 | 0.8391 | 0.7765 | 0.6523 | 0.8268 | 0.8478 | 0.7345 | 0.8566 |
None | 0.0126 | 0.0054 | 0.0025 | 0.0132 | 0.0078 | 0.0013 | 0.0094 | 0.0006 | 0.0019 | 0.0046 | 0.0022 | 0.0055 | 0.0079 | 0.0033 | 0.0039 | 0.0003 | 0.0005 | 0.0063 |
Nuclear Periphery | 0.0186 | 0.004 | 0.0029 | 0.0014 | 0.0015 | 0.0036 | 0.0065 | 0.0005 | 0.0009 | 0.0038 | 0.0137 | 0.0005 | 0.0022 | 0.0003 | 0.0005 | 0.0001 | 0.0001 | 0.0002 |
Nucleolus | 0.0016 | 0.0068 | 0.0041 | 0.0006 | 0.0014 | 0.0004 | 0.0034 | 0.0015 | 0.0007 | 0.001 | 0.0006 | 0.0004 | 0.0008 | 0.0011 | 0.0006 | 0.0003 | 0.0005 | 0.0006 |
Nucleus | 0.0048 | 0.0011 | 0.0017 | 0.0009 | 0.001 | 0.0007 | 0.0033 | 0.0011 | 0.0006 | 0.0021 | 0.0022 | 0.0004 | 0.0007 | 0.0006 | 0.0036 | 0.0003 | 0.0005 | 0.0005 |
Peroxisomes | 0.0138 | 0.0204 | 0.0243 | 0.0118 | 0.0676 | 0.0313 | 0.0439 | 0.0465 | 0.023 | 0.018 | 0.0349 | 0.0321 | 0.0144 | 0.0441 | 0.015 | 0.0197 | 0.0272 | 0.0103 |
Punctate Nuclear | 0.0043 | 0.0004 | 0.0008 | 0.0031 | 0.0042 | 0.0012 | 0.0082 | 0.0002 | 0.0022 | 0.0043 | 0.0056 | 0.0004 | 0.0004 | 0.0003 | 0.0004 | 0.0001 | 0.0001 | 0.0004 |
Vacuole | 0.0083 | 0.0119 | 0.0197 | 0.0055 | 0.0079 | 0.0044 | 0.0122 | 0.0252 | 0.0143 | 0.0081 | 0.0055 | 0.0035 | 0.0153 | 0.0089 | 0.0067 | 0.0044 | 0.0089 | 0.0028 |
Vacuole Periphery | 0.0076 | 0.0085 | 0.0104 | 0.0046 | 0.0048 | 0.0052 | 0.0064 | 0.0107 | 0.0062 | 0.0064 | 0.004 | 0.0039 | 0.0096 | 0.0053 | 0.0046 | 0.0033 | 0.0066 | 0.0037 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 42.5751 | 52.8605 | 53.3456 | 45.1031 | 25.3002 | 43.7335 | 66.5417 | 56.9957 | 61.7622 | 43.8868 |
Translational Efficiency | 0.6078 | 0.6605 | 0.5503 | 0.7189 | 0.8604 | 0.7097 | 0.5902 | 0.8936 | 0.7473 | 0.7171 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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