ORF
Human Ortholog
Description Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole, while HA-tagged protein is found in the soluble fraction, suggesting cytoplasmic localization
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0.08 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Cytoplasm 0.28 0.39 0.47 0.71 0.65 0.8 0.88 – 0.84 0.88 0.8 0.81 – – – 0.59 0.65 0.57 0.07 0.08 0.06 0.44 0.33 0.47
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0.08 0 0 0 0 0 0.08 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0.05 0 0 0 0 0 0.07 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Mitochondria 0.19 0 0 0 0 0 0 – 0 0 0.21 0.1 – – – 0 0 0 0.05 0 0 0 0 0.06
Nucleus 0.14 0.12 0.05 0 0 0 0 – 0 0 0 0 – – – 0.09 0.06 0 0.05 0.06 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0.16 0 0
Vac/Vac Membrane 0.6 0.67 0.53 0.32 0.46 0.32 0.19 – 0.18 0.12 0.12 0.14 – – – 0.41 0.36 0.45 0.74 0.76 0.79 0 0.23 0.21
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 1 1 0 0 0 0
Bud 0 0 0 0 0 0 0 – 1 0 0 0 – – – 0 0 0 4 1 2 0 0 0
Bud Neck 1 0 0 0 0 0 0 – 0 0 0 0 – – – 1 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 1 0 – – – 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Cytoplasm 85 81 27 53 113 156 218 – 170 181 129 155 – – – 101 77 65 22 18 7 3 6 14
Endoplasmic Reticulum 5 2 1 2 0 0 3 – 2 4 4 2 – – – 7 9 3 3 0 1 0 1 0
Endosome 2 4 0 0 1 0 1 – 1 0 0 1 – – – 4 6 5 5 4 0 0 1 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 1 1 2 0 0 0 0
Mitochondria 57 2 0 1 1 2 1 – 7 5 34 19 – – – 2 2 1 15 5 3 0 0 1
Nucleus 41 25 3 2 3 4 2 – 4 7 1 7 – – – 16 7 5 15 13 2 0 0 0
Nuclear Periphery 3 0 0 0 0 0 0 – 0 1 2 2 – – – 0 0 0 3 0 0 0 0 0
Nucleolus 1 0 0 0 0 0 0 – 0 0 0 0 – – – 1 0 0 1 2 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 1 0 0 0
SpindlePole 1 0 0 0 0 0 0 – 0 0 1 1 – – – 0 0 0 5 0 1 1 0 0
Vac/Vac Membrane 179 140 31 24 80 62 46 – 36 24 19 27 – – – 70 43 52 241 170 92 0 4 6
Unique Cell Count 299 209 58 75 173 194 248 203 205 161 192 171 119 115 324 225 117 9 21 32
Labelled Cell Count 375 254 62 82 198 224 271 221 222 191 214 202 144 132 324 225 117 9 21 32


Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.2 5.6 5.8 5.2 6.3 5.1 4.9 – 4.6 4.6 4.2 4.6 – – – 6.5 6.6 6.7 – – –
Std Deviation (1e-4) 0.8 1.2 1.2 1.4 1.9 1.7 1.0 – 1.0 1.3 1.4 1.6 – – – 2.2 2.3 2.8 – – –
Intensity Change (Log2) – – – -0.16 0.12 -0.17 -0.25 – -0.32 -0.34 -0.46 -0.34 – – – 0.17 0.19 0.21 – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Bud 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Cytoplasm 2.8 2.5 5.1 7.1 – 5.8 6.9 4.8 5.1 – – – 1.7 2.3 1.2
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Mitochondria 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Nucleus 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 – – – 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Vacuole -2.5 -1.0 -3.0 -5.5 – -5.5 -6.9 -6.5 -6.2 – – – -1.7 -2.2 -1.0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.3827 -0.0114 -0.2203 -0.7681 -0.3394 -0.2745 -0.8181 -0.2515 -0.52 -0.3012 -0.1704 -0.143 -1.4963 -0.7717 -0.9716 -0.9145 -1.3816 -1.1104
Actin 0.013 0.001 0.0059 0.0001 0.0237 0.0159 0.0399 0.0002 0.0056 0.0194 0.0518 0.0338 0.018 0.0001 0.0311 0 0 0.0001
Bud 0.002 0.0043 0.0035 0.0004 0.0056 0.0036 0.0005 0.0015 0.0014 0.0002 0.0033 0.0004 0.0003 0.0033 0.0058 0.0002 0.0002 0.0008
Bud Neck 0.0061 0.0008 0.0049 0.0007 0.0274 0.0033 0.0041 0.0003 0.0083 0.001 0.0007 0.0009 0.0002 0.0003 0.0016 0.0001 0.0002 0.0004
Bud Periphery 0.0068 0.0078 0.0085 0.001 0.0073 0.0059 0.0007 0.0037 0.0028 0.0002 0.015 0.0012 0.0003 0.0015 0.0129 0.0002 0.0002 0.0004
Bud Site 0.0075 0.0127 0.0122 0.0014 0.0073 0.0008 0.0067 0.0014 0.0044 0.0003 0.0018 0.0006 0.0006 0.0073 0.009 0.0001 0.0001 0.0001
Cell Periphery 0.0006 0.0007 0.0008 0.0003 0.0007 0.0003 0.0005 0.0006 0.0005 0.0002 0.0008 0.0003 0.0003 0.0004 0.0004 0.0001 0.0001 0.0002
Cytoplasm 0.0892 0.1624 0.1063 0.1419 0.1001 0.2146 0.0742 0.0873 0.0775 0.0691 0.1109 0.1559 0.2438 0.2769 0.2766 0.2153 0.2993 0.4886
Cytoplasmic Foci 0.0657 0.0384 0.0543 0.0657 0.0614 0.0791 0.0437 0.0311 0.0472 0.0146 0.0125 0.0332 0.0446 0.0238 0.0332 0.0302 0.0528 0.051
Eisosomes 0.0004 0.0002 0.0003 0.0001 0.0007 0.0002 0.0004 0.0001 0.0002 0.0005 0.0009 0.0002 0.0001 0.0001 0.0002 0 0 0
Endoplasmic Reticulum 0.0035 0.0027 0.0037 0.0024 0.0024 0.0027 0.0065 0.0012 0.0033 0.0082 0.0029 0.0033 0.0051 0.0031 0.0097 0.0028 0.0013 0.0032
Endosome 0.0538 0.05 0.0485 0.0555 0.0812 0.0681 0.0538 0.0243 0.0398 0.0461 0.0357 0.0312 0.0376 0.0334 0.0682 0.2344 0.1511 0.0776
Golgi 0.0074 0.001 0.0051 0.0009 0.0087 0.0055 0.0118 0.0007 0.0068 0.0022 0.005 0.0089 0.0039 0.001 0.0061 0.0016 0.0009 0.0026
Lipid Particles 0.0157 0.0029 0.0167 0.0035 0.0168 0.0152 0.0158 0.0043 0.015 0.0026 0.0014 0.0114 0.0087 0.0018 0.0097 0.0005 0.0019 0.0153
Mitochondria 0.0108 0.0024 0.0078 0.0047 0.0419 0.0082 0.004 0.0024 0.0096 0.0015 0.0063 0.0055 0.0014 0.0033 0.0057 0.0025 0.0012 0.0096
None 0.648 0.6482 0.6472 0.6718 0.5565 0.5152 0.6815 0.7862 0.7044 0.7502 0.7099 0.6325 0.5562 0.5388 0.4002 0.2891 0.3591 0.2364
Nuclear Periphery 0.0106 0.002 0.0042 0.0023 0.004 0.0033 0.0043 0.0009 0.0027 0.0068 0.0025 0.0073 0.0023 0.0019 0.0119 0.0075 0.0013 0.0019
Nucleolus 0.0067 0.0022 0.0033 0.002 0.0047 0.0008 0.0019 0.0013 0.0013 0.0005 0.0006 0.0024 0.0005 0.0007 0.0005 0.0002 0.0004 0.0007
Nucleus 0.0057 0.0056 0.0076 0.0059 0.0028 0.0069 0.004 0.0019 0.0027 0.0026 0.0015 0.0069 0.002 0.0032 0.0047 0.0027 0.0011 0.0034
Peroxisomes 0.0096 0.0017 0.0108 0.0046 0.0092 0.0133 0.0143 0.0015 0.0126 0.0005 0.0003 0.0071 0.0087 0.0045 0.008 0.0001 0.0005 0.0026
Punctate Nuclear 0.0059 0.0027 0.0093 0.0031 0.002 0.0041 0.0046 0.0011 0.0037 0.003 0.0003 0.0099 0.002 0.001 0.0029 0.0003 0.0007 0.001
Vacuole 0.0266 0.0459 0.0331 0.0298 0.0273 0.028 0.0245 0.0449 0.0445 0.0558 0.0326 0.0431 0.059 0.0892 0.0925 0.1634 0.1001 0.0913
Vacuole Periphery 0.0045 0.0045 0.0062 0.002 0.0084 0.0048 0.0022 0.0031 0.0056 0.0143 0.0031 0.0041 0.0044 0.0044 0.0089 0.0487 0.0275 0.0125

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 3.3547 4.9073 4.0461 5.1785 1.5817 3.0481 6.8914 5.5815 6.6039 5.4804
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1385 1402 219 1310 1894 1723 1679 2616 3279 3125 1898 3926

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 676.71 729.90 861.02 808.90 729.67 738.80 816.10 867.58 707.30 734.81 821.28 848.00
Standard Deviation 85.98 107.74 85.83 100.06 81.36 93.92 94.28 118.85 87.35 100.45 94.44 116.27
Intensity Change Log 2 — 0.109161 0.347509 0.257424 — 0.017940 0.161502 0.249753 — 0.062553 0.253999 0.253449

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000023 0.000086 0.000460 0.000380 0.000020 0.000069 0.000105 0.000304 0.000021 0.000077 0.000146 0.000330
Bud Neck 0.029528 0.025149 0.002420 0.008753 0.015749 0.021208 0.003946 0.021080 0.021569 0.022976 0.003770 0.016967
Bud Site 0.009887 0.015874 0.005437 0.039350 0.004403 0.018390 0.006150 0.048356 0.006719 0.017261 0.006068 0.045351
Cell Periphery 0.001175 0.000227 0.000124 0.000225 0.000260 0.000260 0.000104 0.000171 0.000647 0.000245 0.000106 0.000189
Cytoplasm 0.110191* 0.136824* 0.242244* 0.459468* 0.141324* 0.090205 0.437119* 0.627224* 0.128174* 0.111120* 0.414634* 0.571248*
Cytoplasmic Foci 0.028347 0.060939 0.007889 0.020525 0.036000 0.030614 0.010737 0.004217 0.032767 0.044219 0.010409 0.009658
Eisosomes 0.000045 0.000049 0.000058 0.000046 0.000044 0.000031 0.000032 0.000014 0.000044 0.000040 0.000035 0.000025
Endoplasmic Reticulum 0.001651 0.001758 0.006565 0.002023 0.001934 0.001923 0.001547 0.000391 0.001815 0.001849 0.002126 0.000936
Endosome 0.033930 0.071750 0.002398 0.006450 0.032902 0.047609 0.001877 0.001786 0.033336 0.058440 0.001937 0.003342
Golgi 0.001081 0.002777 0.000083 0.000693 0.000220 0.002492 0.000088 0.001170 0.000584 0.002620 0.000087 0.001011
Lipid Particles 0.001721 0.002839 0.001982 0.001446 0.002071 0.001834 0.000936 0.000269 0.001923 0.002285 0.001057 0.000662
Mitochondria 0.001597 0.003562 0.000455 0.002014 0.000571 0.004979 0.000628 0.004934 0.001005 0.004343 0.000608 0.003959
Mitotic Spindle 0.000136 0.002033 0.001117 0.022198 0.000169 0.002167 0.004952 0.038253 0.000155 0.002107 0.004510 0.032896
None 0.002166 0.000922 0.004872 0.005604 0.001853 0.001568 0.006320 0.006862 0.001985 0.001278 0.006153 0.006443
Nuclear Periphery 0.000145 0.000276 0.001648 0.001543 0.000327 0.000242 0.001579 0.000858 0.000250 0.000257 0.001587 0.001087
Nuclear Periphery Foci 0.000105 0.000189 0.001762 0.001207 0.000124 0.000102 0.000545 0.000072 0.000116 0.000141 0.000685 0.000451
Nucleolus 0.000902 0.001450 0.000544 0.000638 0.000667 0.000976 0.000584 0.000445 0.000767 0.001189 0.000579 0.000509
Nucleus 0.024258 0.025907 0.635297* 0.249900* 0.046978 0.023379 0.472649* 0.178487* 0.037382 0.024513 0.491416* 0.202315*
Peroxisomes 0.001180 0.003617 0.000308 0.000992 0.000733 0.001846 0.000341 0.000638 0.000922 0.002641 0.000337 0.000756
Vacuole 0.744668* 0.630726* 0.084145 0.175356 0.708620* 0.735476* 0.049505 0.063513 0.723846* 0.688481* 0.053502 0.100832
Vacuole Periphery 0.007265 0.013046 0.000193 0.001187 0.005030 0.014631 0.000255 0.000955 0.005974 0.013920 0.000248 0.001033

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.19 -7.59 -9.48 -6.33 3.07 -3.44 -12.84 -8.08 -4.74 -1.98 -4.67 -14.01 -12.16 -7.41 -1.99
Bud Neck 1.57 11.25 9.70 10.43 -4.74 -3.19 8.92 0.33 3.63 -9.26 -0.90 13.34 6.83 8.44 -9.11
Bud Site -3.40 1.84 -3.68 -1.53 -4.39 -8.09 -0.73 -9.13 -1.00 -8.22 -8.58 1.53 -9.02 -1.88 -9.51
Cell Periphery 4.33 4.79 4.66 1.98 -0.98 0.02 6.97 6.91 4.53 0.89 4.19 5.84 5.72 5.23 -0.84
Cytoplasm -3.65 -5.47 -28.05 -25.01 -10.82 7.98 -28.05 -52.06* -61.56* -17.05 3.42 -30.92 -58.83 -62.76 -15.13
Cytoplasmic Foci -9.22 9.91 6.31 15.05 -5.81 2.03 14.27 17.66 13.40 6.84 -5.36 16.57 18.17 20.73 1.79
Eisosomes -0.99 -3.97 -2.74 -1.09 2.84 3.14 0.63 8.01 5.24 13.08 1.55 -0.33 6.11 4.08 8.07
Endoplasmic Reticulum -0.37 -9.06 -5.05 -5.60 7.70 0.05 -4.25 11.95 8.95 14.06 -0.22 -8.24 3.30 3.65 11.88
Endosome -11.16 17.84 16.79 23.00 -1.28 -6.38 24.81 25.47 23.72 3.71 -12.62 30.43 30.15 32.87 0.23
Golgi -2.13 2.00 1.59 3.90 -2.55 -4.87 4.42 -1.10 4.12 -1.88 -4.69 2.42 0.83 5.55 -2.38
Lipid Particles -2.62 -1.40 1.19 3.61 2.61 0.56 5.80 9.89 4.67 8.76 -1.46 5.05 8.44 6.33 5.22
Mitochondria -2.26 2.90 0.93 2.92 -2.68 -5.31 -0.14 -5.23 2.59 -5.17 -5.59 2.44 -3.57 3.47 -5.61
Mitotic Spindle -2.40 -1.20 -6.31 -5.10 -3.98 -3.55 -4.61 -11.28 -9.74 -7.73 -4.17 -4.78 -12.92 -10.97 -8.30
None 1.73 -1.62 -2.52 -7.54 -0.76 0.33 -4.02 -8.50 -9.87 -4.76 1.22 -4.57 -8.82 -13.40 -3.31
Nuclear Periphery -3.28 -15.60 -25.39 -22.72 -1.36 2.29 -23.02 -20.66 -21.21 -0.70 -0.28 -27.08 -30.16 -29.23 -1.62
Nuclear Periphery Foci -2.43 -7.74 -14.68 -11.48 2.96 0.79 -12.60 -0.82 -1.40 12.58 -1.64 -14.76 -13.62 -9.73 6.21
Nucleolus -2.84 2.89 3.35 5.18 0.49 -3.33 1.84 -0.75 2.83 -2.36 -4.14 3.00 2.00 5.63 -1.75
Nucleus -0.63 -32.46 -36.43 -35.89 17.55 8.10 -61.22* -46.70 -53.92 24.72 6.32 -70.11 -60.22 -64.76 29.47
Peroxisomes -4.70 3.04 2.44 6.98 -1.26 -5.32 4.26 3.59 7.01 -0.68 -6.65 4.43 3.84 9.55 -1.09
Vacuole 9.83 40.15* 42.03* 31.04* -9.40 -2.66 77.56* 77.99* 77.56* -3.98 4.69 96.26* 87.39* 78.14* -13.34
Vacuole Periphery -5.79 10.72 9.93 16.64 -2.14 -10.50 15.99 14.91 16.40 -2.17 -11.89 17.49 16.41 23.04 -2.92
ORF
Human Ortholog
Description Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole, while HA-tagged protein is found in the soluble fraction, suggesting cytoplasmic localization
Localization
Cell Percentages vacuole (84%)
Cell Cycle Regulation No
Subcompartmental Group N/A

YHR202W

YHR202W


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Yhr202w-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available