Standard name
Human Ortholog
Description Alpha-1,2-specific exomannosidase of the endoplasmic reticulum; involved in glycan trimming of both folded and misfolded glycoproteins; complexes with Pdi1p, and trims a mannose from Man8GlcNac2 glycans to generate Man7GlcNac2, an oligosaccharide signal on glycoproteins destined for ER-associated protein degradation; requires Pdi1p for stability and substrate recognition; human homolog EDEM1 can complement yeast null mutant
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0.05 0 0 – 0 0 0 0.08 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm 0.95 0.98 0.99 0.98 0.97 0.94 0.93 – 0.87 0.81 0.7 0.76 0.99 0.99 1.0 – 0.97 0.97 0.76 0.81 0.75 0.74 0.65 0.63
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0.05 0 0 0 0 0.05 0.09 – 0.22 0.27 0.39 0.16 0 0 0 – 0 0 0.11 0.07 0.12 0.13 0.16 0.15
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.05 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 1 1 0 0 0 – 1 0 3 2 2 7 9 18
Bud 1 2 0 0 0 0 4 – 8 12 7 7 3 0 0 – 0 0 1 3 0 5 14 24
Bud Neck 0 1 9 4 16 7 18 – 10 17 12 23 1 0 0 – 2 3 3 2 1 4 6 12
Bud Site 0 0 0 0 0 0 0 – 1 0 3 1 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 1 6 2 13 3 2 – 3 2 4 1 0 0 0 – 2 3 1 3 3 8 9 14
Cytoplasm 287 397 272 177 305 221 507 – 329 333 218 233 620 809 913 – 418 401 226 328 183 325 386 443
Endoplasmic Reticulum 2 0 1 0 0 3 1 – 0 0 4 9 1 0 0 – 17 25 2 0 1 5 9 10
Endosome 0 0 0 0 0 0 2 – 0 0 0 0 0 1 1 – 1 2 5 4 1 2 5 6
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 1 1 0 0 3 2
Mitochondria 15 11 0 1 1 12 47 – 83 109 123 50 4 4 1 – 7 8 32 28 28 55 93 103
Nucleus 1 1 1 1 0 1 0 – 0 3 1 4 1 2 3 – 2 0 0 1 1 0 0 0
Nuclear Periphery 0 1 0 0 0 0 0 – 1 0 0 0 0 0 0 – 0 0 0 0 0 0 1 3
Nucleolus 0 0 0 0 0 0 0 – 0 0 1 0 1 0 0 – 0 0 0 0 0 0 1 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 1 0 – 1 0 0 1 0 0 1 1
SpindlePole 1 0 2 2 0 2 9 – 7 11 5 11 0 0 0 – 2 2 4 6 3 1 6 13
Vac/Vac Membrane 1 1 0 0 0 0 2 – 1 4 1 0 1 4 0 – 7 7 6 10 5 14 28 36
Unique Cell Count 303 405 275 181 313 236 545 380 411 312 305 624 814 914 431 412 300 406 244 442 593 707
Labelled Cell Count 308 415 291 187 335 249 592 443 491 380 340 632 821 918 460 451 300 406 244 442 593 707


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.5 3.3 4.3 4.0 4.4 3.4 3.6 – 3.0 3.1 2.9 3.4 3.5 3.7 3.8 4.8 5.2 4.9 3.4 3.6 4.0
Std Deviation (1e-4) 0.5 0.7 0.9 1.3 1.1 1.0 1.1 – 0.7 0.7 0.7 0.4 0.6 1.0 1.1 0.8 1.3 1.1 1.0 1.4 1.5
Intensity Change (Log2) – – – -0.12 0.05 -0.35 -0.25 – -0.5 -0.48 -0.58 -0.33 -0.3 -0.21 -0.16 0.15 0.29 0.2 -0.35 -0.25 -0.11


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.6633 1.4325 1.8296 1.5747 0.764 1.5452 -1.3664 -1.2552 -1.1211 -0.6558 -1.2819 -1.3077 -1.4591 -1.0252 -1.0468 -1.0422 -1.7333 -1.3355
Actin 0.0251 0.0003 0.0172 0.0009 0.0041 0.0007 0.0136 0.0017 0.0093 0.0029 0.0211 0.013 0.0105 0.0001 0.0184 0.0002 0 0.0053
Bud 0.0004 0.0002 0.0006 0.0001 0.0001 0.0001 0.003 0.0042 0.0032 0.0081 0.0012 0.0012 0.0002 0 0.0022 0.0001 0 0.0001
Bud Neck 0.0011 0.0005 0.0056 0.0003 0.0003 0.0003 0.0018 0.0002 0.0013 0.001 0.0003 0.0024 0.0007 0 0.0004 0.0006 0 0.0149
Bud Periphery 0.0006 0.0006 0.0012 0.0002 0.0001 0.0004 0.009 0.0058 0.0056 0.0227 0.0027 0.0053 0.0003 0 0.003 0 0 0.0001
Bud Site 0.0076 0.001 0.0089 0.0008 0.0007 0.0004 0.0093 0.0105 0.0147 0.0471 0.0024 0.0005 0.0013 0.0001 0.0035 0.005 0 0.0002
Cell Periphery 0.0004 0.0003 0.0008 0.0001 0.0001 0.0001 0.0005 0.0002 0.0004 0.0007 0.0003 0.0001 0.0002 0.0001 0.0002 0 0 0
Cytoplasm 0.0143 0.0281 0.0188 0.0195 0.0584 0.0248 0.0534 0.0773 0.0607 0.0458 0.0758 0.0891 0.03 0.0797 0.0656 0.0319 0.0707 0.1235
Cytoplasmic Foci 0.0183 0.0024 0.007 0.0108 0.0039 0.006 0.0223 0.0037 0.0142 0.0152 0.0039 0.0181 0.0271 0.0022 0.0101 0.0218 0.002 0.0062
Eisosomes 0.0009 0.0003 0.0005 0.0002 0.0001 0.0001 0.0004 0.0002 0.0003 0.0002 0.0005 0.0001 0.0002 0.0002 0.0003 0.0001 0.0001 0.0001
Endoplasmic Reticulum 0.0016 0.0003 0.0024 0.0007 0.0002 0.0003 0.0033 0.0017 0.0021 0.0018 0.0046 0.0009 0.0041 0.0006 0.0013 0.0004 0.0009 0.0007
Endosome 0.011 0.0001 0.0092 0.0372 0.0011 0.0053 0.0209 0.0047 0.0105 0.006 0.0178 0.0033 0.036 0.0002 0.021 0.0033 0.0003 0.0019
Golgi 0.0068 0.0001 0.0049 0.0118 0.0034 0.0012 0.0065 0.0004 0.0057 0.0021 0.0068 0.0021 0.005 0 0.01 0.001 0 0.002
Lipid Particles 0.0078 0.0002 0.01 0.0042 0.006 0.0023 0.0083 0.0003 0.0051 0.0077 0.0032 0.0053 0.0165 0.0003 0.0025 0.0012 0.0001 0.0014
Mitochondria 0.0135 0.0002 0.0176 0.0335 0.0243 0.0086 0.0079 0.001 0.0228 0.0183 0.0247 0.0038 0.0042 0.0001 0.0147 0.0001 0.0001 0.0038
None 0.8542 0.9639 0.8789 0.8737 0.8831 0.9391 0.8026 0.868 0.8188 0.794 0.8077 0.8458 0.8482 0.9155 0.8397 0.9257 0.9252 0.8375
Nuclear Periphery 0.0069 0.0001 0.0017 0.0005 0.0001 0.0008 0.0048 0.0067 0.0045 0.0015 0.0185 0.0007 0.0033 0.0001 0.0008 0.0002 0.0001 0.0003
Nucleolus 0.0014 0.0002 0.0006 0.0001 0.0001 0.0008 0.0008 0.001 0.0016 0.0021 0.0003 0.0002 0.001 0.0001 0.0002 0.0003 0 0
Nucleus 0.0045 0.0002 0.0011 0.0002 0.0002 0.0012 0.0077 0.0053 0.0039 0.0013 0.002 0.0006 0.0006 0.0002 0.0004 0.0005 0.0001 0.0003
Peroxisomes 0.0165 0.0003 0.0054 0.0023 0.0132 0.0013 0.015 0.0007 0.0085 0.0094 0.0008 0.0038 0.0033 0 0.0022 0.0008 0 0.0006
Punctate Nuclear 0.0054 0.0004 0.0013 0.0002 0.0001 0.0049 0.0026 0.0052 0.0043 0.0011 0.0009 0.0027 0.0039 0.0001 0.0006 0.006 0.0001 0.0004
Vacuole 0.0009 0.0002 0.0058 0.0014 0.0002 0.001 0.005 0.0009 0.0018 0.0076 0.0022 0.0006 0.0026 0.0004 0.0023 0.0005 0.0001 0.0006
Vacuole Periphery 0.0008 0 0.0006 0.0012 0.0001 0.0003 0.0014 0.0004 0.0008 0.0034 0.0023 0.0002 0.0008 0 0.0006 0 0 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 25.3815 23.9272 20.2039 20.2533 21.54 21.2164 15.6418 15.7654 15.5036 26.3257
Translational Efficiency 0.3581 0.2745 0.2499 0.3164 0.354 0.4506 0.6633 0.2535 0.612 0.2906

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1294 1249 270 1373 1449 1333 1471 113 2743 2582 1741 1486

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 604.45 678.46 808.37 773.63 666.95 714.60 787.49 846.75 637.47 697.12 790.73 779.19
Standard Deviation 62.39 98.60 141.29 101.57 75.02 85.21 87.74 124.13 76.04 93.68 98.27 105.25
Intensity Change Log 2 — 0.166641 0.419393 0.356021 — 0.099557 0.239683 0.344357 — 0.131839 0.327916 0.349914

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000066 0.000189 0.000486 0.000768 0.000185 0.000657 0.000103 0.000219 0.000129 0.000431 0.000162 0.000727
Bud Neck 0.024232 0.022154 0.002899 0.010076 0.010266 0.050832 0.002984 0.004489 0.016854 0.036960 0.002970 0.009651
Bud Site 0.002946 0.007417 0.003949 0.041576 0.003840 0.016605 0.006800 0.026169 0.003418 0.012160 0.006358 0.040405
Cell Periphery 0.000195 0.000102 0.000284 0.000360 0.000154 0.000176 0.000160 0.000201 0.000174 0.000140 0.000180 0.000348
Cytoplasm 0.466530* 0.502714* 0.179014* 0.423775* 0.476195* 0.363440* 0.430575* 0.351434* 0.471636* 0.430811* 0.391562* 0.418274*
Cytoplasmic Foci 0.235780* 0.172988 0.017075 0.028481 0.239899* 0.249945* 0.019313 0.036867 0.237956* 0.212718* 0.018966 0.029119
Eisosomes 0.000294 0.000089 0.000089 0.000063 0.000154 0.000237 0.000042 0.000088 0.000220 0.000165 0.000050 0.000065
Endoplasmic Reticulum 0.000853 0.000812 0.005722 0.003459 0.000749 0.001015 0.001495 0.002348 0.000798 0.000917 0.002150 0.003375
Endosome 0.015999 0.011932 0.004186 0.007904 0.009824 0.031978 0.002234 0.017415 0.012737 0.022281 0.002537 0.008627
Golgi 0.001976 0.001875 0.000150 0.002450 0.002122 0.007608 0.000203 0.003373 0.002053 0.004835 0.000195 0.002521
Lipid Particles 0.016600 0.006291 0.003146 0.001715 0.009326 0.011931 0.001244 0.002343 0.012757 0.009203 0.001539 0.001763
Mitochondria 0.005999 0.006322 0.002336 0.002968 0.004319 0.019050 0.000930 0.021093 0.005111 0.012893 0.001148 0.004346
Mitotic Spindle 0.000502 0.002276 0.003412 0.032240 0.001822 0.007319 0.004243 0.019199 0.001199 0.004880 0.004114 0.031248
None 0.001825 0.005230 0.014890 0.003033 0.004351 0.002601 0.004672 0.015534 0.003160 0.003873 0.006256 0.003984
Nuclear Periphery 0.000643 0.000804 0.002028 0.001707 0.000514 0.000513 0.001412 0.001053 0.000575 0.000654 0.001508 0.001657
Nuclear Periphery Foci 0.000249 0.000531 0.001287 0.001165 0.000279 0.000336 0.000523 0.001317 0.000265 0.000430 0.000641 0.001176
Nucleolus 0.001219 0.002475 0.002057 0.000606 0.000965 0.002184 0.000481 0.000315 0.001085 0.002325 0.000726 0.000584
Nucleus 0.162099* 0.189161* 0.695904* 0.307261* 0.197578* 0.127473 0.468682* 0.330202* 0.180841* 0.157314* 0.503920* 0.309005*
Peroxisomes 0.006529 0.007150 0.000602 0.001137 0.004824 0.020740 0.000572 0.000519 0.005629 0.014166 0.000576 0.001090
Vacuole 0.054546 0.057727 0.059962 0.126817 0.031903 0.082303 0.052890 0.164389 0.042585 0.070415 0.053986 0.129674
Vacuole Periphery 0.000916 0.001762 0.000520 0.002440 0.000731 0.003057 0.000444 0.001434 0.000818 0.002430 0.000455 0.002364

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.61 -5.72 -6.19 -4.64 0.20 -1.95 0.56 -0.94 1.68 -3.59 -2.40 -1.94 -5.04 -1.28 -4.47
Bud Neck 0.81 9.94 7.43 5.94 -4.80 -13.33 8.04 6.68 15.63 -0.44 -9.54 12.55 6.85 14.38 -6.29
Bud Site -4.04 -1.44 -8.68 -6.71 -7.89 -6.65 -1.15 -1.52 0.81 -1.19 -7.71 -1.73 -8.56 -5.03 -7.44
Cell Periphery 10.22 -1.95 -0.88 -2.60 0.54 -1.49 0.50 -0.04 0.77 -0.31 3.98 0.43 -1.23 -1.91 -1.32
Cytoplasm -3.00 21.93 11.76 13.64 -13.33 11.08 9.98 7.93 3.53 4.04 5.73 15.68 14.98 9.48 -0.03
Cytoplasmic Foci 8.29 39.19 39.37 27.46 -3.28 -1.11 37.61 27.12 27.65 -1.14 4.44 54.23 52.08 44.11 -3.73
Eisosomes 10.08 8.38 10.98 2.83 4.53 -4.12 12.76 4.22 6.50 -4.09 3.91 15.04 13.61 8.81 -4.33
Endoplasmic Reticulum 0.16 -9.74 -9.22 -9.28 5.94 -0.91 -8.35 -6.34 -5.32 -2.99 -0.68 -12.76 -10.32 -9.43 -1.54
Endosome 2.76 8.84 8.54 6.25 -0.40 -10.88 8.84 0.49 6.47 -1.93 -7.35 14.00 8.18 13.93 -4.30
Golgi 0.35 6.50 1.76 1.40 -2.75 -6.11 8.28 0.83 4.68 -1.06 -5.43 10.06 2.27 6.18 -2.86
Lipid Particles 7.16 9.75 11.51 6.44 4.82 -2.13 13.10 9.38 8.14 -2.28 3.76 15.92 15.74 11.07 -0.85
Mitochondria -0.10 2.43 3.35 4.17 -0.03 -6.86 4.01 -0.45 1.50 -0.94 -5.73 5.46 2.92 8.14 -2.04
Mitotic Spindle -1.87 -1.43 -7.43 -6.53 -4.44 -3.26 -2.20 -0.91 -0.02 -0.35 -3.72 -2.97 -7.20 -5.50 -5.17
None -4.60 -2.60 -2.76 3.54 2.36 2.03 0.47 -0.89 -1.40 -1.03 -1.27 -2.15 0.35 1.77 2.51
Nuclear Periphery -0.57 -9.86 -12.05 -5.67 2.21 -0.05 -14.62 -6.76 -7.18 1.07 -0.57 -17.36 -15.88 -10.77 -1.77
Nuclear Periphery Foci -2.89 -7.66 -9.34 -5.17 2.28 -0.89 -8.00 -4.29 -4.06 -3.11 -2.83 -11.32 -10.15 -7.55 -3.92
Nucleolus -3.74 -1.31 4.44 6.63 4.23 -4.09 5.40 6.76 6.46 1.81 -5.56 4.40 5.97 8.70 1.91
Nucleus -3.94 -30.04 -19.49 -14.45 19.52 10.39 -31.37 -5.91 -8.30 3.76 4.52 -42.31 -18.97 -21.36 18.32
Peroxisomes -0.60 6.89 6.65 8.35 -1.36 -9.91 10.72 11.65 13.10 0.61 -8.51 10.97 10.57 14.98 -1.67
Vacuole -1.67 -4.11 -22.85 -21.50 -11.77 -13.59 -14.21 -8.64 -6.77 -6.43 -11.11 -12.74 -26.61 -21.59 -17.51
Vacuole Periphery -2.01 3.24 -1.03 0.99 -2.49 -8.00 2.70 0.38 5.57 -0.77 -6.49 3.86 -1.30 3.34 -2.85
Standard name
Human Ortholog
Description Alpha-1,2-specific exomannosidase of the endoplasmic reticulum; involved in glycan trimming of both folded and misfolded glycoproteins; complexes with Pdi1p, and trims a mannose from Man8GlcNac2 glycans to generate Man7GlcNac2, an oligosaccharide signal on glycoproteins destined for ER-associated protein degradation; requires Pdi1p for stability and substrate recognition; human homolog EDEM1 can complement yeast null mutant
Localization
Cell Percentages cytoplasm (10%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Mnl1

Mnl1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mnl1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available