Standard name
Human Ortholog
Description Nuclear response regulator and transcription factor; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; part of a branched two-component signaling system; required for optimal induction of heat-shock genes in response to oxidative stress; involved in osmoregulation; relocalizes to the cytosol in response to hypoxia; SKN7 has a paralog, HMS2, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 – 0.06 0.06 0.07 0 – 0.05 0 0 0.05 0.1 0.15 0.11 0.19 0.12 0.08 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.08 0 – 0 0 0.06 0.08 – 0.23 0.15 0.35 0.22 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.95 0.9 – 0.93 0.9 0.87 0.9 – 0.81 0.81 0.79 0.78 0.9 0.82 0.85 0.71 0.78 0.79 0.9 0.86 0.82 0.87 0.8 0.76
Nuclear Periphery 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.05 0 – 0 0 0 0 – 0.07 0.08 0.11 0.09 0 0 0 0 0 0 0 0 0 0 0 0.07
Peroxisomes 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0.06 – 0 0 0 0 – 0 0 0 0 0 0 0 0.07 0.06 0.09 0 0 0 0.05 0.08 0.1
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Bud 0 0 – 0 0 0 0 – 0 1 1 2 0 0 0 0 0 1 1 2 0 0 1 1
Bud Neck 0 0 – 0 0 0 0 – 0 1 1 1 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 1 0 – 0 0 0 1 – 5 0 4 6 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Cytoplasm 0 8 – 13 22 20 20 – 17 17 12 23 6 21 28 27 29 23 0 1 0 1 7 3
Endoplasmic Reticulum 1 1 – 1 0 1 0 – 0 1 0 0 0 1 1 5 7 7 0 0 0 0 0 0
Endosome 0 1 – 1 3 1 0 – 1 2 1 0 0 1 1 3 5 9 1 3 1 0 1 3
Golgi 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 1 1 3 0 0 0 0 0 0
Mitochondria 14 2 – 3 0 19 34 – 75 60 99 94 1 0 0 3 8 7 0 0 1 0 3 3
Nucleus 159 179 – 215 323 261 399 – 267 331 220 339 52 115 220 103 182 216 154 187 36 73 227 241
Nuclear Periphery 0 0 – 1 1 1 0 – 0 2 1 6 0 0 1 0 1 0 0 0 0 1 0 0
Nucleolus 9 0 – 2 5 4 17 – 22 32 31 40 1 2 3 3 6 5 6 7 1 1 9 20
Peroxisomes 0 0 – 0 0 1 0 – 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0
SpindlePole 1 0 – 1 4 3 5 – 2 11 1 9 0 1 2 1 2 2 1 2 0 0 4 4
Vac/Vac Membrane 1 12 – 1 12 5 7 – 6 6 1 2 0 2 9 10 13 26 5 8 1 4 24 32
Unique Cell Count 168 200 231 359 301 445 330 409 280 432 58 141 259 145 232 275 172 217 44 85 286 316
Labelled Cell Count 186 203 238 370 316 483 395 464 372 522 60 144 265 156 255 300 172 217 44 85 286 316


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.5 6.8 – 6.9 7.4 5.9 5.8 – 5.2 5.5 4.9 5.2 6.8 7.2 6.9 9.6 9.3 9.2 5.9 6.7 6.8
Std Deviation (1e-4) 0.8 1.4 – 1.3 1.4 1.4 1.4 – 1.3 1.3 1.0 1.1 1.6 1.4 1.2 2.5 2.3 2.5 1.0 1.1 1.0
Intensity Change (Log2) – – – – – – – – – – – – – – – – – – – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.5284 3.0046 2.4606 2.5859 1.6415 2.3031 3.4414 3.4373 3.3012 3.1479 3.7972 3.2138 2.4147 2.256 2.0204 2.1507 1.3942 1.9563
Actin 0.0169 0.004 0.0011 0.0001 0.0009 0.0008 0.0453 0 0.0198 0.1079 0.0001 0.0007 0.035 0 0.0288 0 0.0004 0.0005
Bud 0.0004 0.0001 0.0002 0.0007 0.0003 0.0002 0.0016 0 0.0003 0.0001 0.0001 0.0001 0.0005 0 0.0004 0 0.0005 0.0003
Bud Neck 0.0023 0.0001 0.0006 0.0061 0.0015 0.0017 0.0053 0 0.0004 0.0001 0.0007 0.0005 0.0013 0 0.0023 0 0.0012 0.0009
Bud Periphery 0.0011 0.0002 0.0005 0.0017 0.0006 0.0007 0.0024 0 0.0004 0.0001 0.0001 0.0003 0.0008 0 0.0006 0 0.0009 0.0005
Bud Site 0.0029 0.0003 0.0014 0.0066 0.0005 0.0007 0.0216 0 0.0047 0.0001 0.0002 0.0003 0.0029 0 0.0022 0 0.0032 0.0005
Cell Periphery 0.0004 0.0002 0.0002 0.0016 0.0002 0.0002 0.0005 0 0.0001 0 0 0.0002 0.0003 0 0.0002 0 0.0009 0.0002
Cytoplasm 0.0045 0.0001 0.0006 0.0003 0.0027 0.0004 0.0089 0 0.0126 0 0.0002 0.0001 0.0068 0 0.0013 0 0.0024 0.0002
Cytoplasmic Foci 0.0082 0.0004 0.0019 0.001 0.0101 0.0043 0.0239 0 0.0066 0.0001 0 0.0045 0.0146 0.0001 0.0061 0 0.0123 0.0004
Eisosomes 0.0003 0.0012 0 0 0 0 0.0005 0 0.0005 0.0002 0 0.0001 0.0004 0 0.0002 0 0 0
Endoplasmic Reticulum 0.003 0.0003 0.0008 0.0002 0.0005 0.0003 0.0032 0 0.0033 0 0 0.0002 0.0031 0 0.0009 0 0.0009 0.0001
Endosome 0.019 0.0005 0.004 0.0016 0.035 0.0043 0.0238 0 0.0099 0.0001 0.0001 0.0083 0.0253 0 0.0113 0 0.0201 0.0009
Golgi 0.0045 0.0008 0.0019 0.0003 0.0021 0.0013 0.0139 0 0.0057 0.0015 0 0.0057 0.0101 0 0.0082 0 0.005 0.0005
Lipid Particles 0.0192 0.002 0.0059 0.0008 0.0245 0.0084 0.0322 0 0.0041 0.0001 0 0.0171 0.0256 0.0001 0.007 0 0.0392 0.0009
Mitochondria 0.0093 0.0015 0.0101 0.0023 0.0015 0.01 0.0264 0.0001 0.0195 0.0007 0.0006 0.0379 0.0086 0.0001 0.003 0.0001 0.0044 0.0108
None 0.0033 0.0015 0.0002 0.0001 0.0003 0.0002 0.0191 0 0.0018 0 0.0001 0.0002 0.0064 0 0.0032 0 0.0003 0.0001
Nuclear Periphery 0.0121 0.0017 0.0046 0.0005 0.0017 0.0016 0.0153 0.0002 0.0007 0.0006 0.0012 0.0016 0.0139 0.0003 0.0033 0.0004 0.003 0.0002
Nucleolus 0.0291 0.0135 0.0196 0.0086 0.1339 0.0748 0.0109 0.0081 0.0115 0.006 0.0385 0.0346 0.0181 0.0146 0.016 0.0081 0.0781 0.0245
Nucleus 0.8355 0.9697 0.9379 0.9529 0.765 0.8829 0.7176 0.9914 0.8883 0.8821 0.9552 0.8805 0.8001 0.9803 0.8937 0.9911 0.8071 0.9572
Peroxisomes 0.0072 0.0015 0.0007 0.0007 0.0121 0.0014 0.015 0 0.0072 0.0002 0 0.0033 0.0045 0 0.002 0 0.0013 0.0002
Punctate Nuclear 0.0122 0.0003 0.0024 0.0001 0.0037 0.0037 0.0083 0.0001 0.0004 0 0.0026 0.0007 0.0164 0.0044 0.0069 0.0001 0.0025 0.0002
Vacuole 0.0054 0.0001 0.0039 0.0128 0.0024 0.0013 0.0025 0 0.0015 0 0.0002 0.0014 0.0033 0 0.0018 0 0.0129 0.0007
Vacuole Periphery 0.0034 0.0001 0.0012 0.0009 0.0005 0.0006 0.0018 0 0.0008 0 0.0001 0.002 0.0021 0 0.0006 0 0.0033 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 25.386 17.9437 19.5809 23.7353 25.6423 11.8009 21.8782 24.1303 16.7317 29.4725
Translational Efficiency 1.054 1.1459 0.9569 0.626 1.0024 1.9806 0.818 0.6679 0.9528 0.8895

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
431 1224 213 1569 1477 1055 949 343 1908 2279 1162 1912

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 635.88 782.94 1066.67 1117.41 810.66 818.77 1047.17 1169.04 771.18 799.53 1050.74 1126.67
Standard Deviation 80.72 133.33 145.64 150.13 98.82 120.89 146.73 166.42 119.89 128.96 146.73 154.45
Intensity Change Log 2 — 0.300147 0.746287 0.813332 — 0.014361 0.369327 0.528155 — 0.147007 0.547260 0.660503

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000349 0.000493 0.001567 0.001369 0.000059 0.000520 0.001016 0.001099 0.000124 0.000506 0.001117 0.001321
Bud Neck 0.001968 0.006738 0.014773 0.045102 0.006038 0.008411 0.020834 0.023929 0.005119 0.007512 0.019723 0.041304
Bud Site 0.002237 0.001464 0.002002 0.010855 0.000366 0.002230 0.001933 0.002924 0.000788 0.001819 0.001945 0.009432
Cell Periphery 0.000124 0.000129 0.000078 0.000265 0.000100 0.000125 0.000083 0.000095 0.000105 0.000127 0.000082 0.000234
Cytoplasm 0.018515 0.007277 0.001004 0.006446 0.008557 0.003611 0.002717 0.003469 0.010806 0.005580 0.002403 0.005912
Cytoplasmic Foci 0.006421 0.000715 0.000056 0.002118 0.000295 0.002180 0.000167 0.000864 0.001679 0.001394 0.000147 0.001893
Eisosomes 0.000029 0.000021 0.000042 0.000035 0.000010 0.000024 0.000036 0.000032 0.000015 0.000023 0.000037 0.000035
Endoplasmic Reticulum 0.002467 0.004520 0.008509 0.004250 0.002001 0.005935 0.004161 0.002714 0.002106 0.005175 0.004958 0.003975
Endosome 0.000609 0.000616 0.000221 0.004229 0.000409 0.002685 0.000613 0.002283 0.000454 0.001574 0.000542 0.003880
Golgi 0.000580 0.000235 0.000086 0.004458 0.000057 0.001418 0.000260 0.000809 0.000175 0.000783 0.000228 0.003804
Lipid Particles 0.001744 0.000487 0.000274 0.000445 0.000142 0.001051 0.000280 0.000084 0.000503 0.000748 0.000279 0.000380
Mitochondria 0.001974 0.001230 0.001652 0.005756 0.000809 0.003899 0.003771 0.005361 0.001072 0.002465 0.003383 0.005685
Mitotic Spindle 0.000892 0.001514 0.000128 0.053462 0.000115 0.006345 0.010395 0.039759 0.000291 0.003750 0.008513 0.051004
None 0.016735 0.011448 0.000951 0.003841 0.003267 0.003911 0.006045 0.005770 0.006309 0.007959 0.005111 0.004187
Nuclear Periphery 0.000562 0.000503 0.000413 0.005874 0.001374 0.001041 0.001014 0.001407 0.001190 0.000752 0.000904 0.005072
Nuclear Periphery Foci 0.000735 0.000656 0.000116 0.001319 0.000133 0.000586 0.001804 0.001165 0.000269 0.000624 0.001494 0.001291
Nucleolus 0.034495 0.022749 0.005083 0.021285 0.009760 0.018104 0.015373 0.013600 0.015348 0.020599 0.013487 0.019906
Nucleus 0.906600* 0.937677* 0.958073* 0.820113* 0.965920* 0.929410* 0.926981* 0.889261* 0.952520* 0.933850* 0.932680* 0.832518*
Peroxisomes 0.001721 0.000744 0.000389 0.000375 0.000075 0.002042 0.000313 0.000371 0.000447 0.001345 0.000327 0.000374
Vacuole 0.000824 0.000564 0.004424 0.006239 0.000354 0.005329 0.001726 0.003107 0.000460 0.002770 0.002220 0.005677
Vacuole Periphery 0.000418 0.000220 0.000160 0.002162 0.000159 0.001143 0.000478 0.001898 0.000218 0.000647 0.000420 0.002115

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.53 -6.79 -12.14 -16.49 -0.97 -6.35 -21.03 -15.80 -10.56 -5.84 -8.41 -19.96 -26.67 -18.63 -6.75
Bud Neck -4.71 -6.52 -27.26 -23.73 -11.76 -2.69 -13.43 -11.31 -9.61 -3.71 -3.70 -15.01 -28.49 -25.33 -13.59
Bud Site 0.36 -0.39 -3.73 -6.24 -4.88 -3.09 -8.66 -9.07 -4.75 -5.39 -2.32 -4.58 -7.87 -6.72 -6.17
Cell Periphery -0.10 1.57 -1.04 -1.13 -3.05 -0.57 0.94 -2.42 -0.28 -3.74 -0.91 1.63 -2.36 -1.10 -3.41
Cytoplasm 3.37 5.65 4.27 1.81 -6.70 3.84 4.89 3.17 -0.35 -1.44 3.93 7.18 4.81 0.90 -4.18
Cytoplasmic Foci 2.68 3.10 2.86 1.03 -2.26 -3.25 2.13 0.20 3.25 -1.25 0.89 3.33 2.38 2.51 -2.24
Eisosomes 1.99 -2.41 -2.60 -10.97 0.35 -7.82 -19.68 -14.29 -9.39 -4.59 -4.97 -15.35 -18.30 -14.35 -2.54
Endoplasmic Reticulum -3.09 -3.11 -4.22 -2.37 1.59 -7.47 -7.91 -7.30 2.87 0.55 -9.48 -7.27 -10.81 0.29 0.15
Endosome -0.00 2.10 -2.47 -2.33 -3.46 -2.87 -0.47 -1.62 1.90 -1.40 -2.84 0.01 -3.17 0.05 -3.17
Golgi 1.20 1.78 -1.39 -2.08 -2.34 -2.92 -2.08 -4.57 1.62 -2.66 -2.64 -0.32 -2.35 -1.12 -2.29
Lipid Particles 2.60 3.15 3.12 1.04 -0.31 -3.00 -0.96 -2.26 2.60 -0.36 -1.12 2.22 1.54 2.45 -1.12
Mitochondria 1.10 0.26 -4.93 -10.72 -10.05 -3.29 -2.67 -4.84 -0.66 -1.16 -2.72 -2.52 -10.40 -5.80 -3.19
Mitotic Spindle -0.82 0.84 -12.60 -12.39 -12.94 -3.40 -4.16 -6.39 -5.63 -5.28 -3.78 -4.04 -14.41 -13.16 -11.47
None 1.77 5.98 4.57 4.24 -11.88 -0.45 -1.41 -3.07 -2.34 -1.21 -1.24 1.60 1.49 2.82 -0.73
Nuclear Periphery 0.58 1.03 -6.56 -6.65 -6.71 0.92 1.13 -2.23 -3.12 -3.31 1.97 1.09 -5.61 -6.62 -6.20
Nuclear Periphery Foci -0.04 2.52 -1.40 -1.89 -6.82 -1.89 -1.71 -2.26 -0.57 0.58 -2.39 -1.38 -4.78 -2.26 -0.33
Nucleolus 3.04 7.42 -2.78 -7.99 -14.24 -4.61 -4.45 -4.94 -2.95 -2.74 -3.20 -0.55 -11.44 -9.41 -10.32
Nucleus -3.57 -4.28 10.46 19.34 17.33 6.33 9.01 10.75 7.48 6.98 4.41 5.49 24.11 20.57 18.52
Peroxisomes 1.39 1.93 1.79 0.99 -2.13 -2.53 -8.44 -8.25 1.65 -4.29 -1.59 1.06 0.14 2.01 -5.53
Vacuole 0.91 -2.05 -6.73 -7.12 -0.08 -4.42 -5.34 -3.49 -1.56 -2.67 -4.40 -4.28 -8.00 -5.05 -4.05
Vacuole Periphery 0.95 1.15 -1.76 -3.42 -3.70 -3.37 -1.49 -1.79 0.98 -1.05 -2.90 -0.92 -3.70 -1.13 -2.74
Standard name
Human Ortholog
Description Nuclear response regulator and transcription factor; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; part of a branched two-component signaling system; required for optimal induction of heat-shock genes in response to oxidative stress; involved in osmoregulation; relocalizes to the cytosol in response to hypoxia; SKN7 has a paralog, HMS2, that arose from the whole genome duplication
Localization
Cell Percentages nucleus (99%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Skn7

Skn7


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Skn7-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available