Standard name | |
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Human Ortholog | |
Description | Nuclear response regulator and transcription factor; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; part of a branched two-component signaling system; required for optimal induction of heat-shock genes in response to oxidative stress; involved in osmoregulation; relocalizes to the cytosol in response to hypoxia; SKN7 has a paralog, HMS2, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 1 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 0 | 0 | 1 | 1 |
Bud Neck | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 1 | 0 | – | 0 | 0 | 0 | 1 | – | 5 | 0 | 4 | 6 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 8 | – | 13 | 22 | 20 | 20 | – | 17 | 17 | 12 | 23 | 6 | 21 | 28 | 27 | 29 | 23 | 0 | 1 | 0 | 1 | 7 | 3 |
Endoplasmic Reticulum | 1 | 1 | – | 1 | 0 | 1 | 0 | – | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 5 | 7 | 7 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 1 | – | 1 | 3 | 1 | 0 | – | 1 | 2 | 1 | 0 | 0 | 1 | 1 | 3 | 5 | 9 | 1 | 3 | 1 | 0 | 1 | 3 |
Golgi | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 3 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 14 | 2 | – | 3 | 0 | 19 | 34 | – | 75 | 60 | 99 | 94 | 1 | 0 | 0 | 3 | 8 | 7 | 0 | 0 | 1 | 0 | 3 | 3 |
Nucleus | 159 | 179 | – | 215 | 323 | 261 | 399 | – | 267 | 331 | 220 | 339 | 52 | 115 | 220 | 103 | 182 | 216 | 154 | 187 | 36 | 73 | 227 | 241 |
Nuclear Periphery | 0 | 0 | – | 1 | 1 | 1 | 0 | – | 0 | 2 | 1 | 6 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 |
Nucleolus | 9 | 0 | – | 2 | 5 | 4 | 17 | – | 22 | 32 | 31 | 40 | 1 | 2 | 3 | 3 | 6 | 5 | 6 | 7 | 1 | 1 | 9 | 20 |
Peroxisomes | 0 | 0 | – | 0 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 1 | 0 | – | 1 | 4 | 3 | 5 | – | 2 | 11 | 1 | 9 | 0 | 1 | 2 | 1 | 2 | 2 | 1 | 2 | 0 | 0 | 4 | 4 |
Vac/Vac Membrane | 1 | 12 | – | 1 | 12 | 5 | 7 | – | 6 | 6 | 1 | 2 | 0 | 2 | 9 | 10 | 13 | 26 | 5 | 8 | 1 | 4 | 24 | 32 |
Unique Cell Count | 168 | 200 | 231 | 359 | 301 | 445 | 330 | 409 | 280 | 432 | 58 | 141 | 259 | 145 | 232 | 275 | 172 | 217 | 44 | 85 | 286 | 316 | ||
Labelled Cell Count | 186 | 203 | 238 | 370 | 316 | 483 | 395 | 464 | 372 | 522 | 60 | 144 | 265 | 156 | 255 | 300 | 172 | 217 | 44 | 85 | 286 | 316 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.5 | 6.8 | – | 6.9 | 7.4 | 5.9 | 5.8 | – | 5.2 | 5.5 | 4.9 | 5.2 | 6.8 | 7.2 | 6.9 | 9.6 | 9.3 | 9.2 | 5.9 | 6.7 | 6.8 |
Std Deviation (1e-4) | 0.8 | 1.4 | – | 1.3 | 1.4 | 1.4 | 1.4 | – | 1.3 | 1.3 | 1.0 | 1.1 | 1.6 | 1.4 | 1.2 | 2.5 | 2.3 | 2.5 | 1.0 | 1.1 | 1.0 |
Intensity Change (Log2) | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.5284 | 3.0046 | 2.4606 | 2.5859 | 1.6415 | 2.3031 | 3.4414 | 3.4373 | 3.3012 | 3.1479 | 3.7972 | 3.2138 | 2.4147 | 2.256 | 2.0204 | 2.1507 | 1.3942 | 1.9563 |
Actin | 0.0169 | 0.004 | 0.0011 | 0.0001 | 0.0009 | 0.0008 | 0.0453 | 0 | 0.0198 | 0.1079 | 0.0001 | 0.0007 | 0.035 | 0 | 0.0288 | 0 | 0.0004 | 0.0005 |
Bud | 0.0004 | 0.0001 | 0.0002 | 0.0007 | 0.0003 | 0.0002 | 0.0016 | 0 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0005 | 0 | 0.0004 | 0 | 0.0005 | 0.0003 |
Bud Neck | 0.0023 | 0.0001 | 0.0006 | 0.0061 | 0.0015 | 0.0017 | 0.0053 | 0 | 0.0004 | 0.0001 | 0.0007 | 0.0005 | 0.0013 | 0 | 0.0023 | 0 | 0.0012 | 0.0009 |
Bud Periphery | 0.0011 | 0.0002 | 0.0005 | 0.0017 | 0.0006 | 0.0007 | 0.0024 | 0 | 0.0004 | 0.0001 | 0.0001 | 0.0003 | 0.0008 | 0 | 0.0006 | 0 | 0.0009 | 0.0005 |
Bud Site | 0.0029 | 0.0003 | 0.0014 | 0.0066 | 0.0005 | 0.0007 | 0.0216 | 0 | 0.0047 | 0.0001 | 0.0002 | 0.0003 | 0.0029 | 0 | 0.0022 | 0 | 0.0032 | 0.0005 |
Cell Periphery | 0.0004 | 0.0002 | 0.0002 | 0.0016 | 0.0002 | 0.0002 | 0.0005 | 0 | 0.0001 | 0 | 0 | 0.0002 | 0.0003 | 0 | 0.0002 | 0 | 0.0009 | 0.0002 |
Cytoplasm | 0.0045 | 0.0001 | 0.0006 | 0.0003 | 0.0027 | 0.0004 | 0.0089 | 0 | 0.0126 | 0 | 0.0002 | 0.0001 | 0.0068 | 0 | 0.0013 | 0 | 0.0024 | 0.0002 |
Cytoplasmic Foci | 0.0082 | 0.0004 | 0.0019 | 0.001 | 0.0101 | 0.0043 | 0.0239 | 0 | 0.0066 | 0.0001 | 0 | 0.0045 | 0.0146 | 0.0001 | 0.0061 | 0 | 0.0123 | 0.0004 |
Eisosomes | 0.0003 | 0.0012 | 0 | 0 | 0 | 0 | 0.0005 | 0 | 0.0005 | 0.0002 | 0 | 0.0001 | 0.0004 | 0 | 0.0002 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.003 | 0.0003 | 0.0008 | 0.0002 | 0.0005 | 0.0003 | 0.0032 | 0 | 0.0033 | 0 | 0 | 0.0002 | 0.0031 | 0 | 0.0009 | 0 | 0.0009 | 0.0001 |
Endosome | 0.019 | 0.0005 | 0.004 | 0.0016 | 0.035 | 0.0043 | 0.0238 | 0 | 0.0099 | 0.0001 | 0.0001 | 0.0083 | 0.0253 | 0 | 0.0113 | 0 | 0.0201 | 0.0009 |
Golgi | 0.0045 | 0.0008 | 0.0019 | 0.0003 | 0.0021 | 0.0013 | 0.0139 | 0 | 0.0057 | 0.0015 | 0 | 0.0057 | 0.0101 | 0 | 0.0082 | 0 | 0.005 | 0.0005 |
Lipid Particles | 0.0192 | 0.002 | 0.0059 | 0.0008 | 0.0245 | 0.0084 | 0.0322 | 0 | 0.0041 | 0.0001 | 0 | 0.0171 | 0.0256 | 0.0001 | 0.007 | 0 | 0.0392 | 0.0009 |
Mitochondria | 0.0093 | 0.0015 | 0.0101 | 0.0023 | 0.0015 | 0.01 | 0.0264 | 0.0001 | 0.0195 | 0.0007 | 0.0006 | 0.0379 | 0.0086 | 0.0001 | 0.003 | 0.0001 | 0.0044 | 0.0108 |
None | 0.0033 | 0.0015 | 0.0002 | 0.0001 | 0.0003 | 0.0002 | 0.0191 | 0 | 0.0018 | 0 | 0.0001 | 0.0002 | 0.0064 | 0 | 0.0032 | 0 | 0.0003 | 0.0001 |
Nuclear Periphery | 0.0121 | 0.0017 | 0.0046 | 0.0005 | 0.0017 | 0.0016 | 0.0153 | 0.0002 | 0.0007 | 0.0006 | 0.0012 | 0.0016 | 0.0139 | 0.0003 | 0.0033 | 0.0004 | 0.003 | 0.0002 |
Nucleolus | 0.0291 | 0.0135 | 0.0196 | 0.0086 | 0.1339 | 0.0748 | 0.0109 | 0.0081 | 0.0115 | 0.006 | 0.0385 | 0.0346 | 0.0181 | 0.0146 | 0.016 | 0.0081 | 0.0781 | 0.0245 |
Nucleus | 0.8355 | 0.9697 | 0.9379 | 0.9529 | 0.765 | 0.8829 | 0.7176 | 0.9914 | 0.8883 | 0.8821 | 0.9552 | 0.8805 | 0.8001 | 0.9803 | 0.8937 | 0.9911 | 0.8071 | 0.9572 |
Peroxisomes | 0.0072 | 0.0015 | 0.0007 | 0.0007 | 0.0121 | 0.0014 | 0.015 | 0 | 0.0072 | 0.0002 | 0 | 0.0033 | 0.0045 | 0 | 0.002 | 0 | 0.0013 | 0.0002 |
Punctate Nuclear | 0.0122 | 0.0003 | 0.0024 | 0.0001 | 0.0037 | 0.0037 | 0.0083 | 0.0001 | 0.0004 | 0 | 0.0026 | 0.0007 | 0.0164 | 0.0044 | 0.0069 | 0.0001 | 0.0025 | 0.0002 |
Vacuole | 0.0054 | 0.0001 | 0.0039 | 0.0128 | 0.0024 | 0.0013 | 0.0025 | 0 | 0.0015 | 0 | 0.0002 | 0.0014 | 0.0033 | 0 | 0.0018 | 0 | 0.0129 | 0.0007 |
Vacuole Periphery | 0.0034 | 0.0001 | 0.0012 | 0.0009 | 0.0005 | 0.0006 | 0.0018 | 0 | 0.0008 | 0 | 0.0001 | 0.002 | 0.0021 | 0 | 0.0006 | 0 | 0.0033 | 0.0003 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 25.386 | 17.9437 | 19.5809 | 23.7353 | 25.6423 | 11.8009 | 21.8782 | 24.1303 | 16.7317 | 29.4725 |
Translational Efficiency | 1.054 | 1.1459 | 0.9569 | 0.626 | 1.0024 | 1.9806 | 0.818 | 0.6679 | 0.9528 | 0.8895 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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