Standard name
Human Ortholog
Description Phosphatidylinositol 4,5-bisphosphate 5-phosphatase; synaptojanin-like protein with an N-terminal Sac1 domain, plays a role in phosphatidylinositol 4,5-bisphosphate homeostasis and in endocytosis; null mutation confers cold-tolerant growth
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Bud 0 0 0 0 0 0 0 0 0 – 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.05 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.06 0.07 0 0 0 0 0 0
Cytoplasm 0.97 0.95 0.99 0.96 0.99 0.92 0.92 0.86 0.82 – 0.73 0.72 0.99 0.99 0.99 0.97 0.97 0.97 0.9 0.88 0.82 0.85 0.84 0.75
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.05 0 0.08 0 0.18 0.18 0.24 0.35 – 0.42 0.45 0 0 0 0 0 0 0 0 0.09 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 2 0 1 0 – 0 2 0 0 0 0 0 3 3 3 2 6 9 22
Bud 2 2 4 8 4 10 8 14 12 – 12 23 3 4 10 0 0 0 1 0 0 2 4 8
Bud Neck 0 0 1 2 1 3 0 1 1 – 0 0 0 0 0 1 0 0 4 3 5 3 6 12
Bud Site 0 0 0 0 0 0 0 0 1 – 1 3 0 0 0 0 0 0 – – – – – –
Cell Periphery 14 1 6 14 11 10 8 18 7 – 9 15 4 6 9 13 22 31 0 1 4 3 10 14
Cytoplasm 249 154 201 305 398 373 427 624 282 – 235 349 397 543 597 476 337 405 245 146 181 219 313 350
Endoplasmic Reticulum 5 2 0 1 0 1 0 1 1 – 0 2 0 1 4 17 16 16 4 1 2 3 4 8
Endosome 1 1 0 0 0 0 1 4 1 – 0 0 0 0 0 6 1 0 0 3 0 2 0 8
Golgi 2 0 0 1 0 0 0 0 0 – 0 0 1 0 0 0 0 1 1 3 0 0 0 3
Mitochondria 1 8 3 24 15 73 81 173 120 – 137 219 3 2 8 16 12 1 4 1 20 9 10 16
Nucleus 0 0 0 2 3 3 3 2 0 – 1 1 0 2 1 3 2 2 0 0 0 0 0 0
Nuclear Periphery 0 2 0 0 0 0 0 0 0 – 0 2 0 0 1 0 0 0 0 0 0 1 0 0
Nucleolus 1 0 0 1 1 0 1 1 0 – 0 0 1 0 0 0 1 0 0 0 0 0 0 1
Peroxisomes 0 1 0 0 0 0 0 1 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 2
SpindlePole 0 0 0 1 0 0 4 4 0 – 0 0 0 0 1 2 1 1 0 0 0 0 2 1
Vac/Vac Membrane 0 0 0 0 0 0 0 1 2 – 3 4 1 0 0 5 7 3 0 1 2 0 5 2
Unique Cell Count 256 162 204 318 403 405 462 722 346 323 482 400 547 605 489 347 419 273 167 221 259 375 470
Labelled Cell Count 275 171 215 359 434 475 533 845 427 398 620 410 558 631 539 399 463 273 167 221 259 375 470


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.6 4.6 3.9 4.1 4.4 3.7 3.9 3.9 3.3 – 3.5 3.5 5.2 5.3 4.7 6.3 6.9 7.1 5.3 4.8 5.5
Std Deviation (1e-4) 0.5 0.8 0.5 2.8 1.8 1.0 1.4 1.4 0.8 – 0.9 1.1 1.1 1.4 1.2 1.0 1.1 1.1 1.4 1.2 1.3
Intensity Change (Log2) – – – 0.09 0.18 -0.06 0.01 0.01 -0.23 – -0.16 -0.16 0.41 0.46 0.27 0.69 0.82 0.86 0.45 0.29 0.51


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.1379 2.0202 1.6529 1.7263 1.1143 1.7379 1.8205 2.0415 2.2749 1.7168 2.351 1.974 1.4662 2.0171 1.7492 1.7027 1.3692 1.703
Actin 0.0704 0.0126 0.0199 0.0263 0.0024 0.0024 0.0499 0.0021 0.0289 0.0164 0.003 0.0093 0.0139 0.0004 0.0021 0.0217 0.0021 0.008
Bud 0.0019 0.0021 0.0036 0.0015 0.006 0.0003 0.0007 0.0012 0.0039 0.0024 0.0008 0.0021 0.0014 0.0003 0.0008 0.0207 0.0001 0.0016
Bud Neck 0.0057 0.0013 0.0067 0.0061 0.0006 0.0023 0.0031 0.0006 0.009 0.0005 0.0029 0.0016 0.0007 0.0004 0.0025 0.0004 0.0002 0.0021
Bud Periphery 0.004 0.0058 0.0082 0.003 0.0099 0.0012 0.001 0.0013 0.0056 0.0038 0.0012 0.0039 0.0033 0.0004 0.0012 0.0094 0.0002 0.0036
Bud Site 0.0116 0.0305 0.0334 0.0221 0.0165 0.0001 0.0081 0.0257 0.014 0.0021 0.0052 0.0029 0.0235 0.0043 0.0055 0.0019 0.0002 0.0016
Cell Periphery 0.0023 0.0033 0.0013 0.0011 0.0005 0.0009 0.0009 0.0009 0.001 0.0004 0.0003 0.0002 0.0033 0.0021 0.0016 0.0006 0.0002 0.0006
Cytoplasm 0.4598 0.5714 0.5065 0.6132 0.6175 0.7047 0.4422 0.7081 0.4869 0.5912 0.5401 0.7518 0.3669 0.4634 0.461 0.3346 0.5374 0.4859
Cytoplasmic Foci 0.0179 0.005 0.0308 0.0171 0.0228 0.0127 0.0664 0.0081 0.0191 0.02 0.0256 0.0131 0.0082 0.0167 0.0083 0.0436 0.0129 0.0138
Eisosomes 0.0007 0.0003 0.0003 0.0002 0 0.0001 0.0006 0.0001 0.0003 0.0001 0.0001 0.0001 0.0007 0.0003 0.0003 0.0002 0.0002 0.0003
Endoplasmic Reticulum 0.0094 0.0029 0.0027 0.005 0.0015 0.0036 0.0087 0.0025 0.0043 0.0048 0.0091 0.0018 0.0043 0.0034 0.0022 0.0018 0.0012 0.0016
Endosome 0.0332 0.0009 0.0131 0.0024 0.0102 0.0045 0.0345 0.0028 0.0183 0.0234 0.0943 0.0116 0.0034 0.0011 0.0021 0.0139 0.0046 0.0021
Golgi 0.0123 0.0007 0.0038 0.0024 0.0028 0.0036 0.0155 0.0007 0.0077 0.0062 0.0246 0.0036 0.0011 0.0003 0.0005 0.0016 0.0003 0.0012
Lipid Particles 0.0148 0.0005 0.0024 0.0026 0.0015 0.001 0.0384 0.0003 0.0146 0.0024 0.0052 0.0027 0.0019 0.0003 0.0005 0.0008 0.0002 0.0014
Mitochondria 0.0098 0.0003 0.0021 0.0009 0.0373 0.0143 0.0075 0.0011 0.0049 0.0059 0.0082 0.0039 0.0008 0.0001 0.0003 0.0011 0.0002 0.0006
None 0.3159 0.3587 0.352 0.2903 0.2615 0.2393 0.2564 0.2398 0.3491 0.2925 0.2588 0.1804 0.5608 0.503 0.508 0.5195 0.4379 0.4683
Nuclear Periphery 0.008 0.0003 0.0009 0.0003 0.0006 0.0025 0.0166 0.0005 0.0095 0.0015 0.0029 0.0006 0.001 0.0003 0.0004 0.0012 0.0002 0.0005
Nucleolus 0.0011 0.0003 0.0003 0.0002 0.0002 0.0001 0.0058 0.0002 0.0007 0.0003 0.0005 0.0002 0.0003 0.0001 0.0002 0.0007 0.0001 0.0003
Nucleus 0.0029 0.0009 0.0008 0.0007 0.0009 0.0009 0.0024 0.0011 0.008 0.0013 0.0029 0.0007 0.0009 0.0004 0.0006 0.006 0.0004 0.0018
Peroxisomes 0.005 0.0003 0.0072 0.0019 0.0006 0.0036 0.0275 0.0004 0.0067 0.0089 0.0015 0.0069 0.0009 0.0006 0.0003 0.0127 0.0004 0.0019
Punctate Nuclear 0.0061 0.0005 0.002 0.0014 0.0007 0.0004 0.0074 0.0009 0.0019 0.0008 0.0019 0.0009 0.0009 0.0016 0.0005 0.0026 0.0002 0.0022
Vacuole 0.0057 0.0013 0.0016 0.0011 0.0049 0.0011 0.0044 0.0015 0.0044 0.012 0.0085 0.0013 0.0017 0.0005 0.001 0.004 0.0007 0.0006
Vacuole Periphery 0.0015 0.0001 0.0003 0.0001 0.0011 0.0004 0.0021 0.0001 0.0012 0.0031 0.0025 0.0003 0.0002 0 0.0001 0.0007 0.0001 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 6.2142 13.5405 8.5019 15.8589 13.0925 8.9279 13.5153 16.3484 14.3465 15.0528
Translational Efficiency 0.8168 0.3847 0.5471 0.3317 0.3095 0.5509 0.4035 0.3586 0.3824 0.3127

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2076 86 142 1383 1274 1334 122 236 3350 1420 264 1619

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 684.73 842.71 1091.92 860.13 650.89 730.89 965.66 890.52 671.86 737.66 1033.57 864.56
Standard Deviation 75.22 103.18 105.71 100.75 95.62 96.80 94.89 101.92 85.17 100.79 118.89 101.49
Intensity Change Log 2 — 0.299501 0.673260 0.329020 — 0.167241 0.569102 0.452234 — 0.236559 0.623439 0.390381

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000424 0.001205 0.001600 0.002108 0.000450 0.000753 0.000151 0.002037 0.000434 0.000780 0.000931 0.002097
Bud Neck 0.024340 0.016521 0.004963 0.011361 0.024990 0.041857 0.001658 0.014059 0.024587 0.040323 0.003436 0.011754
Bud Site 0.013145 0.019278 0.012594 0.034429 0.009812 0.027236 0.002812 0.028316 0.011877 0.026754 0.008074 0.033538
Cell Periphery 0.000415 0.000683 0.000680 0.000153 0.000248 0.000272 0.000518 0.000134 0.000351 0.000297 0.000605 0.000150
Cytoplasm 0.556311* 0.419483* 0.332840* 0.518201* 0.477274* 0.440595* 0.331788* 0.426914* 0.526253* 0.439316* 0.332354* 0.504894*
Cytoplasmic Foci 0.198187* 0.295355* 0.048593 0.042535 0.279536* 0.245008* 0.112280 0.058992 0.229124* 0.248057* 0.078024 0.044934
Eisosomes 0.000756 0.000717 0.000157 0.000057 0.000746 0.000462 0.000313 0.000088 0.000752 0.000478 0.000229 0.000061
Endoplasmic Reticulum 0.001053 0.000892 0.012566 0.001153 0.001449 0.000709 0.001583 0.001454 0.001204 0.000720 0.007491 0.001197
Endosome 0.013072 0.041937 0.016397 0.011375 0.031011 0.038719 0.014932 0.012987 0.019894 0.038914 0.015720 0.011610
Golgi 0.005014 0.009834 0.000441 0.004419 0.006610 0.009773 0.000180 0.000274 0.005621 0.009777 0.000320 0.003815
Lipid Particles 0.007186 0.007502 0.007055 0.001584 0.013327 0.007917 0.007505 0.001466 0.009521 0.007892 0.007263 0.001567
Mitochondria 0.002970 0.002258 0.000390 0.003734 0.005227 0.008129 0.000148 0.000269 0.003828 0.007773 0.000278 0.003229
Mitotic Spindle 0.000709 0.000685 0.001778 0.046304 0.000500 0.004391 0.004711 0.022382 0.000630 0.004167 0.003134 0.042817
None 0.004110 0.000210 0.000908 0.004358 0.004536 0.001357 0.001415 0.001482 0.004272 0.001287 0.001142 0.003939
Nuclear Periphery 0.000145 0.001346 0.001806 0.000775 0.000543 0.000237 0.000937 0.000591 0.000296 0.000304 0.001404 0.000748
Nuclear Periphery Foci 0.000520 0.004466 0.013941 0.001201 0.000388 0.000506 0.003086 0.001557 0.000470 0.000746 0.008924 0.001253
Nucleolus 0.000758 0.001149 0.000357 0.000449 0.001006 0.000967 0.000921 0.000288 0.000852 0.000978 0.000618 0.000426
Nucleus 0.085816 0.035526 0.190881* 0.088314 0.059013 0.044517 0.246942* 0.065417 0.075623 0.043973 0.216788* 0.084976
Peroxisomes 0.003592 0.012083 0.000809 0.004509 0.006633 0.012458 0.001027 0.002814 0.004749 0.012435 0.000909 0.004262
Vacuole 0.080485 0.127480 0.350749* 0.219476* 0.074468 0.112003 0.266779* 0.356713* 0.078197 0.112941 0.311944* 0.239481*
Vacuole Periphery 0.000992 0.001390 0.000493 0.003506 0.002233 0.002133 0.000315 0.001767 0.001464 0.002088 0.000411 0.003253

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.61 -7.80 -1.45 0.65 2.06 -1.96 1.84 -1.14 -0.74 -1.48 -3.30 -4.62 -1.76 -0.47 -0.08
Bud Neck 3.02 15.32 12.99 3.88 -1.32 -5.81 12.97 6.87 11.36 -1.83 -6.57 20.47 14.87 14.64 -3.30
Bud Site -1.61 1.34 -2.01 0.44 -3.06 -8.13 5.81 -2.29 2.18 -3.99 -7.35 4.30 -3.15 3.69 -5.62
Cell Periphery -1.41 -2.89 11.20 3.08 10.12 -1.23 -4.57 9.39 8.83 7.44 2.13 -4.59 13.50 9.89 12.58
Cytoplasm 5.02 13.90 14.03 -0.29 -6.93 3.42 8.27 7.40 5.51 -1.39 10.31 16.25 13.56 2.61 -7.85
Cytoplasmic Foci -4.51 28.84 43.27 12.74 2.20 4.43 18.45 36.09 30.46 6.58 -2.96 28.65 58.15 39.16 7.26
Eisosomes 0.24 10.75 12.53 3.95 8.66 1.06 1.69 2.47 9.39 8.50 2.43 4.99 6.46 10.88 11.86
Endoplasmic Reticulum 0.49 -11.08 -2.10 -1.99 10.83 2.41 -1.82 -1.98 -6.08 -0.08 2.78 -9.84 -1.99 -7.23 9.55
Endosome -4.49 0.13 8.81 5.75 4.79 -2.78 6.38 11.31 13.82 4.47 -8.94 4.30 14.66 16.61 6.96
Golgi -0.95 14.16 6.29 1.68 -2.30 -3.07 11.92 11.95 10.95 0.57 -4.47 18.48 8.87 8.85 -2.42
Lipid Particles -0.21 1.54 13.11 4.43 8.47 4.13 4.74 11.24 7.69 8.16 1.85 4.34 16.64 8.34 11.79
Mitochondria 0.45 5.74 2.72 0.46 -3.47 -1.82 7.20 7.03 5.52 -1.94 -2.82 9.19 5.38 4.71 -3.88
Mitotic Spindle 0.06 -1.98 -7.83 -7.79 -7.46 -3.12 -1.03 -2.32 -1.62 -1.12 -3.08 -1.25 -8.18 -6.30 -5.40
None 11.25 8.36 1.15 -6.88 -5.18 2.91 3.07 2.86 -0.56 -0.77 4.54 6.66 1.87 -2.94 -4.59
Nuclear Periphery -1.05 -13.33 -20.46 -0.13 4.03 2.21 -3.24 -3.94 -8.86 -0.94 -0.08 -12.26 -14.95 -12.16 1.80
Nuclear Periphery Foci -1.04 -11.91 -5.14 0.87 11.35 -1.11 -6.17 -6.28 -5.45 3.13 -0.99 -12.17 -8.33 -2.17 11.00
Nucleolus -0.50 7.99 6.98 1.08 -0.74 0.28 1.05 5.35 4.42 4.99 -0.61 3.64 7.46 3.85 3.13
Nucleus 6.87 -7.47 -9.18 -11.37 4.89 4.35 -8.29 -4.37 -5.85 5.30 11.70 -10.99 -11.79 -18.31 6.91
Peroxisomes -2.27 9.93 3.58 2.81 -1.97 -4.67 7.10 5.86 9.94 -0.52 -7.49 11.03 5.92 10.31 -1.70
Vacuole -2.94 -14.97 -32.21 -11.82 2.02 -7.61 -10.24 -19.83 -17.78 -3.82 -8.30 -17.64 -37.33 -30.04 0.21
Vacuole Periphery -0.76 3.92 -0.39 0.34 -1.80 0.30 6.94 4.36 5.00 -0.97 -2.95 7.84 0.97 2.50 -1.97
Standard name
Human Ortholog
Description Phosphatidylinositol 4,5-bisphosphate 5-phosphatase; synaptojanin-like protein with an N-terminal Sac1 domain, plays a role in phosphatidylinositol 4,5-bisphosphate homeostasis and in endocytosis; null mutation confers cold-tolerant growth
Localization
Cell Percentages cytoplasm (70%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Inp51

Inp51


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Inp51-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available