Standard name
Human Ortholog
Description Type II membrane protein required for vesicular transport; required for vesicular transport between the endoplasmic reticulum and Golgi complex; v-SNARE with similarity to synaptobrevins
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.1 0.06 0 0 0 0 0 0
Bud 0 0 0 0 0 0.06 0 0.08 0.1 0.07 0.15 0.1 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.8 0.94 0.9 0.87 0.87 0.89 0.77 0.77 0.7 0.63 0.65 0.58 0.88 0.93 0.95 0.83 0.85 0.61 0.2 0.33 0.22 0.18 0.26 0.29
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0.09 0.1 0.08 0 0 0 0 0.05 0
Endosome 0.07 0 0 0.05 0.05 0 0 0 0 0.05 0 0 0 0 0 0 0.05 0.25 0.13 0.06 0.13 0.08 0 0.05
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.08 0 0.19 0.09 0.1 0.15 0.28 0.32 0.42 0.36 0.39 0.43 0 0 0 0.06 0.07 0 0.13 0.11 0.15 0.16 0.15 0.12
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0.06 0 0 0 0 0 0
Vac/Vac Membrane 0.08 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.42 0.44 0.39 0.51 0.4 0.41
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 0 0 0 2 0 3 0 2 0 3 1 1 1 3 4 3 3 0 0 1 1 1
Bud 2 1 0 7 7 15 11 24 27 21 23 27 1 0 1 2 0 0 3 1 0 0 3 4
Bud Neck 0 0 0 1 1 1 0 0 1 0 0 1 0 0 0 0 0 0 1 2 0 1 1 0
Bud Site 1 0 0 0 0 1 2 1 1 0 2 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0 0 1 0 0 0 0 0 0
Cytoplasm 180 211 66 167 242 214 258 234 188 179 100 164 61 154 176 67 35 31 51 76 21 19 31 53
Endoplasmic Reticulum 4 6 0 0 0 1 0 0 1 2 1 6 5 3 3 7 4 4 3 5 3 2 5 7
Endosome 16 1 1 9 15 5 4 7 3 14 5 9 2 3 3 3 2 13 33 13 13 8 3 10
Golgi 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 11 1 1 0 0 2
Mitochondria 19 8 14 18 28 36 92 98 112 102 61 120 0 0 1 5 3 2 32 24 15 17 18 22
Nucleus 0 0 0 0 0 0 4 2 1 4 1 4 1 0 0 0 0 0 0 0 0 0 1 0
Nuclear Periphery 0 1 0 1 0 0 2 0 2 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 2 0 0 0 0 0 0 1 1 0 0 2 0 0 1 0 0 1 0 0 0 0 0 0
Peroxisomes 1 0 0 0 0 0 0 2 2 6 1 5 0 1 0 0 0 1 2 0 1 0 0 0
SpindlePole 3 0 0 3 1 0 9 7 10 7 5 21 0 4 0 2 1 3 2 1 0 0 1 1
Vac/Vac Membrane 19 2 1 3 8 3 4 4 1 8 2 6 1 2 1 1 1 4 107 101 39 55 49 76
Unique Cell Count 224 225 73 191 278 241 333 305 267 284 155 282 69 165 186 81 41 51 257 231 101 110 121 186
Labelled Cell Count 250 230 82 209 302 278 386 383 350 345 201 371 72 168 188 90 51 64 257 231 101 110 121 186


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.7 4.6 3.7 4.0 4.0 3.9 3.6 3.6 3.6 3.7 3.6 4.0 5.5 5.3 5.1 5.4 5.8 6.3 5.7 6.1 6.2
Std Deviation (1e-4) 1.0 0.7 0.9 1.9 1.6 1.6 1.6 1.3 1.3 1.4 1.3 1.4 1.2 1.3 1.2 0.8 1.2 1.2 1.3 1.9 2.2
Intensity Change (Log2) – – – 0.09 0.08 0.07 -0.04 -0.04 -0.04 -0.02 -0.03 0.09 0.57 0.5 0.45 0.53 0.65 0.75 0.62 0.72 0.73


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.6686 0.8374 0.9449 0.5324 0.4236 1.346 1.6344 2.0651 2.1351 2.1995 1.8389 2.1623 -0.4624 0.1891 -0.1095 0.1415 -0.0525 0.1014
Actin 0.0226 0.003 0.0109 0.0007 0.0002 0.0131 0.0399 0.0076 0.0101 0.0139 0.0159 0.0022 0.0049 0.0011 0.0054 0.0016 0.0025 0.0019
Bud 0.0004 0.0012 0.0025 0.0005 0.0004 0.0004 0.0012 0.0014 0.0008 0.0004 0.0002 0.0003 0.0002 0.0003 0.0004 0.0004 0.0002 0.0002
Bud Neck 0.0011 0.0003 0.0005 0.0003 0.0003 0.0023 0.0013 0.0004 0.0005 0.002 0.0003 0.0012 0.0012 0.0003 0.0005 0.0004 0.0006 0.0011
Bud Periphery 0.0005 0.0012 0.0014 0.0008 0.0005 0.001 0.0026 0.0012 0.0016 0.0004 0.0002 0.0003 0.0002 0.0003 0.0004 0.0002 0.0002 0.0001
Bud Site 0.0021 0.0077 0.0068 0.0002 0.0003 0.0006 0.0028 0.0025 0.0016 0.0035 0.0007 0.0003 0.0011 0.0022 0.001 0.0003 0.0003 0.0002
Cell Periphery 0.0002 0.0003 0.0005 0.0001 0 0.0002 0.0008 0.0003 0.0006 0.0002 0.0001 0.0003 0.0006 0.0002 0.0002 0.0001 0.0002 0.0001
Cytoplasm 0.1246 0.2351 0.1257 0.1506 0.115 0.2101 0.1118 0.2197 0.1444 0.0852 0.0239 0.1549 0.184 0.2963 0.1511 0.2314 0.1367 0.2297
Cytoplasmic Foci 0.1508 0.1603 0.179 0.1846 0.1558 0.1468 0.2053 0.2412 0.1825 0.2145 0.2141 0.1708 0.1873 0.1898 0.1839 0.1373 0.2176 0.1954
Eisosomes 0.0004 0.0002 0.0004 0.0001 0 0.0004 0.0012 0.0001 0.0006 0.0001 0.0002 0.0003 0.0012 0.0002 0.0001 0 0.0002 0
Endoplasmic Reticulum 0.0073 0.01 0.0044 0.0027 0.0018 0.0054 0.0118 0.0035 0.0066 0.0027 0.0016 0.0047 0.0127 0.0074 0.0058 0.0099 0.0107 0.0086
Endosome 0.3794 0.3003 0.2717 0.3566 0.4132 0.2612 0.2336 0.2695 0.2774 0.3931 0.535 0.3043 0.3512 0.2381 0.3467 0.3739 0.4606 0.3384
Golgi 0.0517 0.0335 0.0456 0.0574 0.0177 0.0413 0.0687 0.1043 0.0906 0.0886 0.0594 0.0737 0.0561 0.0315 0.0724 0.0659 0.0624 0.0576
Lipid Particles 0.0238 0.0067 0.0125 0.005 0.0012 0.018 0.0499 0.0086 0.0075 0.0169 0.0372 0.0171 0.0168 0.0094 0.0128 0.0021 0.008 0.0093
Mitochondria 0.0159 0.0052 0.01 0.0132 0.0049 0.0098 0.0235 0.0146 0.0181 0.0184 0.0063 0.0294 0.0056 0.0073 0.0126 0.0072 0.0077 0.0081
None 0.1626 0.1996 0.2801 0.1518 0.2218 0.2235 0.149 0.0986 0.2115 0.0985 0.0098 0.2005 0.1497 0.1888 0.1722 0.1225 0.0546 0.1255
Nuclear Periphery 0.0082 0.0024 0.0059 0.0025 0.001 0.0071 0.005 0.0006 0.0012 0.0006 0.0012 0.0008 0.0026 0.0017 0.0017 0.002 0.002 0.0009
Nucleolus 0.0006 0.0004 0.0007 0.0004 0.0001 0.0011 0.0016 0.0001 0.0004 0.0003 0.0002 0.0002 0.0003 0.0002 0.0002 0.0001 0.0001 0.0001
Nucleus 0.002 0.0012 0.0014 0.0007 0.0007 0.0027 0.003 0.0004 0.0007 0.0005 0.0002 0.0005 0.0011 0.0011 0.0011 0.001 0.0009 0.0006
Peroxisomes 0.0183 0.0064 0.0239 0.003 0.0347 0.015 0.0687 0.0169 0.0328 0.0497 0.077 0.028 0.0085 0.0082 0.018 0.0292 0.0216 0.0117
Punctate Nuclear 0.0038 0.0013 0.004 0.0015 0.0005 0.0057 0.0029 0.0004 0.0008 0.0008 0.0029 0.001 0.0012 0.0011 0.0014 0.0005 0.0012 0.0007
Vacuole 0.019 0.019 0.0083 0.0433 0.0243 0.028 0.0131 0.0062 0.0073 0.0064 0.0098 0.0062 0.011 0.0122 0.0089 0.0098 0.0078 0.007
Vacuole Periphery 0.0045 0.0044 0.0038 0.0241 0.0053 0.0062 0.0024 0.0018 0.0025 0.0032 0.0038 0.003 0.0025 0.0023 0.0032 0.0042 0.0039 0.0029

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 12.8602 22.9925 11.943 22.2987 18.3412 7.7118 25.5724 24.3204 30.3043 25.0239
Translational Efficiency 4.0284 2.1971 2.774 1.4234 2.7612 6.7858 2.0325 1.6574 1.2654 2.0742

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
759 1171 1364 1539 918 1796 2758 1960 1677 2967 4122 3499

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 586.66 688.61 830.23 819.98 595.46 686.09 881.05 866.41 591.48 687.08 864.23 845.99
Standard Deviation 79.19 90.46 110.21 116.51 74.38 93.91 103.62 113.43 76.72 92.57 108.52 117.08
Intensity Change Log 2 — 0.231163 0.500986 0.483064 — 0.204393 0.565219 0.541045 — 0.217740 0.533699 0.512562

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000321 0.001485 0.001686 0.001749 0.000297 0.000865 0.001270 0.003095 0.000308 0.001110 0.001407 0.002503
Bud Neck 0.021474 0.015542 0.012751 0.010147 0.011451 0.019105 0.019340 0.012758 0.015988 0.017699 0.017160 0.011610
Bud Site 0.005375 0.010696 0.013712 0.033237 0.002714 0.008005 0.012271 0.035577 0.003919 0.009067 0.012747 0.034548
Cell Periphery 0.000480 0.000109 0.000286 0.000200 0.000142 0.000109 0.000077 0.000137 0.000295 0.000109 0.000146 0.000165
Cytoplasm 0.351310* 0.182807* 0.200677* 0.466738* 0.206515* 0.158156* 0.262396* 0.480342* 0.272048* 0.167885* 0.241973* 0.474359*
Cytoplasmic Foci 0.310405* 0.429827* 0.260241* 0.094005 0.413764* 0.418042* 0.221406* 0.131207 0.366984* 0.422693* 0.234257* 0.114844
Eisosomes 0.000581 0.000243 0.000179 0.000076 0.000300 0.000425 0.000074 0.000094 0.000427 0.000353 0.000108 0.000086
Endoplasmic Reticulum 0.001514 0.001772 0.006500 0.003984 0.002116 0.002270 0.003898 0.001996 0.001843 0.002074 0.004759 0.002870
Endosome 0.037627 0.119543 0.111407 0.021412 0.090689 0.117941 0.099010 0.030889 0.066674 0.118573 0.103112 0.026720
Golgi 0.010755 0.072136 0.023151 0.005261 0.035771 0.077723 0.013506 0.007016 0.024449 0.075518 0.016698 0.006244
Lipid Particles 0.040555 0.033804 0.074924 0.005808 0.076144 0.044399 0.036454 0.007944 0.060037 0.040217 0.049184 0.007005
Mitochondria 0.009261 0.011941 0.005890 0.005809 0.013686 0.026463 0.006942 0.003767 0.011683 0.020732 0.006594 0.004665
Mitotic Spindle 0.000522 0.004812 0.034750 0.026598 0.001969 0.003252 0.065432 0.021355 0.001314 0.003868 0.055279 0.023661
None 0.004458 0.002150 0.004018 0.002348 0.004494 0.000320 0.001839 0.001265 0.004478 0.001042 0.002560 0.001741
Nuclear Periphery 0.000842 0.000466 0.003378 0.001589 0.001399 0.000284 0.005522 0.000989 0.001147 0.000356 0.004812 0.001253
Nuclear Periphery Foci 0.000350 0.001519 0.010154 0.003908 0.001867 0.000239 0.006237 0.001455 0.001181 0.000744 0.007533 0.002534
Nucleolus 0.001453 0.001701 0.005840 0.000614 0.005563 0.000529 0.003771 0.000563 0.003703 0.000991 0.004455 0.000585
Nucleus 0.102797 0.016848 0.104816 0.149846* 0.055672 0.015137 0.084973 0.081842 0.077001 0.015812 0.091539 0.111753
Peroxisomes 0.017564 0.027063 0.021627 0.007722 0.025764 0.022955 0.020248 0.015944 0.022053 0.024576 0.020704 0.012327
Vacuole 0.079711 0.059345 0.100547 0.155555 0.046284 0.076985 0.133279 0.160093 0.061413 0.070023 0.122448 0.158097
Vacuole Periphery 0.002641 0.006192 0.003467 0.003395 0.003397 0.006797 0.002057 0.001673 0.003055 0.006558 0.002524 0.002430

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.55 -3.72 -3.98 0.32 0.72 -5.59 -5.29 -4.01 -2.97 -2.35 -5.55 -6.21 -5.11 -2.59 -1.90
Bud Neck 2.19 3.47 5.34 4.49 3.28 -4.94 -6.43 0.05 5.76 8.00 -1.22 -1.25 4.53 7.43 8.94
Bud Site -4.07 -4.54 -7.67 -5.55 -4.00 -7.68 -9.14 -11.71 -8.99 -7.11 -7.45 -9.03 -13.79 -10.52 -8.16
Cell Periphery 4.67 2.39 3.99 -2.96 3.26 2.14 6.28 1.92 -0.64 -5.41 5.01 4.10 4.28 -2.59 0.17
Cytoplasm 14.25 12.76 -6.53 -24.30 -22.93 5.37 -6.15 -25.25 -36.71 -22.90 14.04 3.69 -22.20 -43.83 -31.12
Cytoplasmic Foci -11.52 5.58 27.17 48.41 28.11* -0.42 24.79 37.40 49.58 18.46 -8.17 22.17 44.83 69.52 31.84*
Eisosomes 4.83 6.07 7.66 7.41 11.69 -3.09 6.96 6.61 13.57 -1.97 1.85 9.11 9.93 15.43 6.52
Endoplasmic Reticulum -0.80 -8.20 -6.56 -5.81 3.87 -0.45 -5.26 0.38 0.88 7.22 -0.88 -9.92 -4.25 -2.97 6.58
Endosome -15.10 -13.51 6.99 22.22 21.15* -4.95 0.09 14.40 25.42 21.23* -13.23 -7.93 15.54 33.99 30.19*
Golgi -14.60 -4.55 4.46 17.43 9.76 -10.00 7.71 9.82 23.68 5.64 -17.13 4.84 10.64 29.41 10.93
Lipid Particles 1.57 -6.57 11.46 11.48 18.53 6.36 9.25 15.89 15.54 15.96 5.91 4.34 19.27 19.36 24.33
Mitochondria -1.69 2.70 3.65 5.95 1.73 -6.18 5.00 7.35 14.18 5.81 -6.52 5.50 7.99 15.10 5.22
Mitotic Spindle -4.92 -10.57 -8.83 -6.55 3.58 -1.26 -19.94* -8.20 -7.35 12.49 -3.70 -22.63 -11.99 -9.87 12.72
None 1.42 0.11 1.39 -0.24 2.39 5.54 3.35 4.21 -9.75 2.12 4.17 2.15 3.40 -2.92 2.78
Nuclear Periphery 1.99 -7.20 -4.84 -8.17 3.98 4.14 -8.67 0.49 -10.29 10.24 4.65 -11.05 -2.43 -12.55 10.62
Nuclear Periphery Foci -2.20 -10.96 -8.72 -3.23 7.00 4.93 -6.84 1.27 -9.15 8.34 1.59 -12.42 -4.68 -5.96 10.25
Nucleolus -0.59 -5.82 1.78 3.16 8.16 4.56 1.37 4.67 0.29 13.23 4.11 -1.40 4.98 2.56 14.79
Nucleus 16.18 -0.44 -9.86 -30.23 -9.58 10.13 -7.33 -8.46 -24.95 -1.56 18.55 -4.70 -12.56 -38.43 -9.04
Peroxisomes -3.41 -1.51 5.88 10.53 10.97 1.06 2.56 4.99 5.45 3.94 -1.36 1.19 7.35 11.06 9.25
Vacuole 3.24 -4.14 -17.05 -23.86 -15.29 -7.65 -22.65 -27.42 -21.88 -9.03 -2.33 -18.17 -30.61 -31.97 -16.00
Vacuole Periphery -4.35 -1.48 0.77 4.31 1.80 -6.75 4.60 5.07 11.73 2.05 -7.95 2.90 3.76 10.31 2.14
Standard name
Human Ortholog
Description Type II membrane protein required for vesicular transport; required for vesicular transport between the endoplasmic reticulum and Golgi complex; v-SNARE with similarity to synaptobrevins
Localization
Cell Percentages —
Cell Cycle Regulation No
Subcompartmental Group N/A

Bet1

Bet1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Bet1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available