Standard name
Human Ortholog
Description Ubiquitin-like protein involved in thiolation of cytoplasmic tRNAs; receives sulfur from the E1-like enzyme Uba4p and transfers it to tRNA; also functions as a protein tag with roles in nutrient sensing and oxidative stress response
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – – – – –
Cell Periphery 0 0.08 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Cytoplasm 0.98 0.92 0.94 0.93 0.94 0.95 – 0.92 0.88 0.94 0.84 0.82 0.98 0.98 0.98 – – – 0.93 0.95 0.84 0.95 0.92 0.93
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – 0 0 0.05 0 0 0
Mitochondria 0 0.08 0 0 0 0.1 – 0 0.08 0.05 0.1 0.21 0 0 0 – – – 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0.06 0 0 0 – 0 0.05 0 0 0 0 0 0 – – – 0 0 0.07 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – 1 0 0 0 0 0
Bud 0 0 0 1 0 0 – 2 0 0 0 1 0 0 1 – – – 0 0 0 0 1 1
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – – – – –
Cell Periphery 0 3 0 0 1 2 – 3 0 1 2 2 0 0 1 – – – 0 0 0 0 0 0
Cytoplasm 129 34 16 128 151 120 – 246 75 92 43 67 130 234 213 – – – 124 57 31 33 152 180
Endoplasmic Reticulum 1 0 0 2 0 0 – 1 0 1 0 0 1 2 2 – – – 0 0 0 0 2 0
Endosome 1 0 0 0 0 0 – 1 1 0 0 0 0 0 0 – – – 1 0 0 0 1 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 1 0 – – – 0 0 1 0 2 2
Mitochondria 1 3 0 6 7 13 – 10 7 5 5 17 1 2 1 – – – 0 0 0 0 0 0
Nucleus 1 1 0 1 1 0 – 0 1 0 2 0 1 0 0 – – – 2 0 0 0 0 1
Nuclear Periphery 1 0 0 1 1 0 – 0 0 0 0 1 0 0 0 – – – 0 0 0 0 0 0
Nucleolus 0 0 0 0 1 0 – 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Vac/Vac Membrane 0 0 1 6 4 0 – 9 4 2 2 2 0 2 2 – – – 0 0 2 0 1 1
Unique Cell Count 132 37 17 138 161 126 266 85 98 51 82 132 238 218 134 61 38 35 165 194
Labelled Cell Count 134 41 17 145 166 135 272 88 101 54 90 133 241 220 134 61 38 35 165 194


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.0 11.2 7.2 7.2 7.1 6.3 – 6.8 6.4 6.6 7.0 6.3 10.4 10.9 10.2 – – – – 12.1 12.0
Std Deviation (1e-4) 1.4 2.1 1.2 1.4 1.5 1.4 – 1.8 1.6 1.6 2.1 1.7 1.8 1.8 1.8 – – – – 2.2 2.3
Intensity Change (Log2) – – – -0.01 -0.02 -0.19 – -0.08 -0.18 -0.12 -0.05 -0.2 0.52 0.59 0.49 – – – – 0.75 0.74


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 – – –
Bud 0 0 0 – 0 0 0 0 0 0 0 0 – – –
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 – – –
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 – – –
Cell Periphery 0 0 0 – 0 0 0 0 0 0 0 0 – – –
Cytoplasm -0.2 -0.1 0.2 – -0.2 -0.7 0 -1.0 -1.3 1.2 1.2 0.9 – – –
Endoplasmic Reticulum 0 0 0 – 0 0 0 0 0 0 0 0 – – –
Endosome 0 0 0 – 0 0 0 0 0 0 0 0 – – –
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 – – –
Mitochondria 0 0 0 – 0 0 0 0 0 0 0 0 – – –
Nucleus 0 0 0 – 0 0 0 0 0 0 0 0 – – –
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 – – –
Nucleolus 0 0 0 – 0 0 0 0 0 0 0 0 – – –
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 – – –
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 – – –
Vacuole 0 0 0 – 0 0 0 0 0 0 0 0 – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.4102 6.5559 6.0505 5.3339 4.4401 5.9723 3.9881 6.7531 5.9689 6.167 4.2698 6.0564 7.6879 10.1419 9.2312 9.1843 7.3534 9.371
Actin 0.0534 0.0075 0.0223 0.0005 0.0425 0.0078 0.1403 0.0029 0.0415 0.0001 0.2193 0.0004 0.0145 0.0064 0.0009 0.1074 0.0011 0.0001
Bud 0.0008 0.0002 0.0002 0.0001 0.0011 0.0002 0.0008 0.0006 0.0003 0 0.0091 0.0002 0.0003 0.0003 0.0002 0.0003 0.0016 0
Bud Neck 0.0024 0.0003 0.0004 0.0003 0.0005 0.0012 0.0028 0.0001 0.0012 0.0002 0.0008 0.0011 0.0004 0.0004 0.0115 0.0006 0.0004 0.0006
Bud Periphery 0.0009 0.0001 0.0001 0 0.0043 0.0003 0.0008 0.0001 0.0002 0 0.0077 0.0001 0.0004 0.0003 0.0001 0.0003 0.0017 0
Bud Site 0.0043 0.0008 0.001 0.0007 0.0012 0.0003 0.0045 0.0007 0.0008 0 0.0076 0.0007 0.0008 0.0034 0.0031 0.0007 0.0045 0
Cell Periphery 0.0001 0 0 0 0.0003 0 0.0004 0 0.0001 0 0.0001 0 0.0001 0.0001 0.0001 0 0.0001 0
Cytoplasm 0.7994 0.9708 0.9296 0.8964 0.7387 0.8983 0.563 0.9876 0.9062 0.9914 0.6987 0.8856 0.9014 0.9516 0.9463 0.8464 0.7922 0.9896
Cytoplasmic Foci 0.0217 0.0026 0.0108 0.0474 0.0107 0.0049 0.046 0.0015 0.0046 0.0007 0.0083 0.0467 0.0021 0.0074 0.0077 0.0094 0.0076 0.0007
Eisosomes 0.0003 0.0001 0.0001 0 0.0007 0.0001 0.0004 0 0.0001 0 0.0002 0 0.0004 0 0 0.0001 0 0
Endoplasmic Reticulum 0.0054 0.0012 0.0027 0.0016 0.0012 0.0014 0.0481 0.0006 0.0007 0.0003 0.0005 0.0014 0.003 0.0018 0.0005 0.0013 0.0027 0.0003
Endosome 0.0221 0.0012 0.0078 0.0022 0.0033 0.0072 0.0688 0.0002 0.0159 0.0002 0.0019 0.0305 0.0065 0.0073 0.0151 0.0039 0.039 0.0001
Golgi 0.0133 0.0015 0.0016 0.0011 0.005 0.0121 0.0334 0.0001 0.009 0 0.0091 0.003 0.0038 0.0009 0.0027 0.005 0.0019 0
Lipid Particles 0.0097 0.0005 0.0016 0.0031 0.0227 0.0128 0.0243 0 0.0009 0 0.0004 0.0021 0.0078 0.0057 0.0015 0.0012 0.0027 0
Mitochondria 0.0057 0.0016 0.0003 0.0001 0.1084 0.0262 0.0123 0.0001 0.0039 0 0.0119 0.0016 0.0271 0.0013 0.0002 0.0018 0.0052 0.0001
None 0.0283 0.0055 0.0056 0.0032 0.0017 0.0034 0.0064 0.0027 0.002 0.0006 0.0018 0.0018 0.0112 0.0023 0.0011 0.0014 0.0143 0.0011
Nuclear Periphery 0.0058 0.0007 0.0037 0.0008 0.001 0.002 0.017 0.0003 0.0007 0.0005 0.001 0.0027 0.0094 0.0024 0.0006 0.0011 0.0303 0.0003
Nucleolus 0.0006 0 0.0001 0.0003 0.0002 0.0001 0.0006 0 0 0 0 0.0003 0.0002 0.001 0.0002 0 0.0088 0
Nucleus 0.0059 0.0043 0.0038 0.0029 0.0038 0.0172 0.0074 0.0019 0.0077 0.0055 0.0125 0.0088 0.006 0.0029 0.0054 0.0105 0.052 0.0067
Peroxisomes 0.0087 0.0004 0.005 0.0047 0.0489 0.0023 0.0141 0.0002 0.0027 0 0.0081 0.0074 0.0014 0.0022 0.0017 0.0076 0.0019 0
Punctate Nuclear 0.0093 0.0004 0.0027 0.0342 0.0006 0.0011 0.004 0.0001 0.0006 0.0002 0.0004 0.0024 0.0008 0.0017 0.0003 0.0007 0.0177 0.0001
Vacuole 0.0013 0.0003 0.0005 0.0002 0.0013 0.0007 0.0031 0.0001 0.0008 0.0001 0.0003 0.0022 0.001 0.0004 0.0007 0.0001 0.0096 0.0001
Vacuole Periphery 0.0006 0.0001 0.0002 0.0001 0.0019 0.0005 0.0014 0 0.0003 0 0.0001 0.0009 0.0014 0.0002 0.0001 0.0001 0.0046 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 40.4582 32.8792 39.0365 56.1743 45.3027 51.4637 54.0217 78.5314 49.7769 47.3211
Translational Efficiency 3.1367 4.7642 3.2142 1.9894 2.4029 3.0257 2.4418 1.6612 2.0902 3.0077

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
83 1308 1511 1447 1156 161 394 195 1239 1469 1905 1642

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 790.60 980.04 1281.52 1266.00 860.46 1011.58 1357.62 1360.04 855.78 983.50 1297.26 1277.17
Standard Deviation 99.75 147.66 193.22 203.61 130.82 143.59 175.89 225.97 130.15 147.55 192.25 208.62
Intensity Change Log 2 — 0.309893 0.696836 0.679258 — 0.233430 0.657900 0.660469 — 0.270550 0.676675 0.669496

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000278 0.000365 0.000905 0.002854 0.000254 0.000423 0.000783 0.002089 0.000255 0.000371 0.000880 0.002763
Bud Neck 0.004126 0.002592 0.004910 0.010019 0.001996 0.004828 0.008989 0.005150 0.002139 0.002837 0.005753 0.009441
Bud Site 0.015095 0.011885 0.014285 0.025571 0.005750 0.017554 0.015151 0.019288 0.006376 0.012507 0.014464 0.024825
Cell Periphery 0.000156 0.000072 0.000141 0.000194 0.000126 0.000480 0.000184 0.000350 0.000128 0.000117 0.000150 0.000213
Cytoplasm 0.652706* 0.792671* 0.524505* 0.503631* 0.715334* 0.633161* 0.366489* 0.440231* 0.711139* 0.775189* 0.491824* 0.496102*
Cytoplasmic Foci 0.163246 0.053144 0.006622 0.011511 0.096068 0.113383 0.008681 0.020269 0.100568 0.059746 0.007048 0.012551
Eisosomes 0.000049 0.000013 0.000011 0.000014 0.000032 0.000056 0.000015 0.000035 0.000033 0.000018 0.000012 0.000016
Endoplasmic Reticulum 0.001274 0.000428 0.000964 0.001215 0.001273 0.001813 0.001724 0.013195 0.001273 0.000580 0.001121 0.002637
Endosome 0.007776 0.002275 0.002652 0.004819 0.004486 0.011917 0.006369 0.032793 0.004706 0.003332 0.003421 0.008141
Golgi 0.000510 0.000805 0.001587 0.013578 0.001495 0.002456 0.002078 0.013851 0.001429 0.000986 0.001688 0.013611
Lipid Particles 0.001988 0.000548 0.000759 0.001132 0.001410 0.003366 0.001564 0.004453 0.001448 0.000857 0.000925 0.001526
Mitochondria 0.000435 0.003536 0.002630 0.004515 0.003235 0.004189 0.004971 0.014060 0.003048 0.003608 0.003114 0.005648
Mitotic Spindle 0.000347 0.008178 0.034481 0.081316 0.003323 0.020028 0.078184 0.047609 0.003124 0.009477 0.043520 0.077313
None 0.001987 0.004222 0.009549 0.005531 0.003283 0.001433 0.005035 0.005937 0.003196 0.003917 0.008615 0.005580
Nuclear Periphery 0.000202 0.000110 0.000683 0.000915 0.000195 0.000241 0.001490 0.002335 0.000195 0.000124 0.000850 0.001083
Nuclear Periphery Foci 0.001443 0.001194 0.001595 0.003516 0.001745 0.002356 0.002648 0.009908 0.001725 0.001322 0.001813 0.004275
Nucleolus 0.000477 0.000217 0.000197 0.000403 0.000426 0.002467 0.000180 0.000107 0.000429 0.000464 0.000194 0.000368
Nucleus 0.079513 0.072139 0.351826* 0.275878* 0.074830 0.054051 0.348903* 0.215451* 0.075143 0.070156 0.351222* 0.268702*
Peroxisomes 0.001255 0.001181 0.000231 0.000973 0.001003 0.005230 0.000589 0.000422 0.001020 0.001624 0.000305 0.000908
Vacuole 0.066461 0.042405 0.040674 0.048061 0.080846 0.117820 0.135423 0.145325 0.079882 0.050670 0.060270 0.059612
Vacuole Periphery 0.000675 0.002019 0.000796 0.004353 0.002890 0.002748 0.010549 0.007141 0.002742 0.002099 0.002813 0.004684

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.93 -3.84 -3.73 -3.38 -1.77 -0.96 -6.21 -3.78 -2.78 -0.28 -1.31 -5.14 -4.16 -3.67 -1.75
Bud Neck 0.86 -0.71 -2.01 -6.00 -2.45 -1.66 -4.45 -6.44 -1.05 1.04 -1.46 -5.96 -7.85 -6.11 -2.06
Bud Site 0.61 1.12 0.18 -1.11 -2.74 -1.99 -3.58 -4.18 -0.34 -1.81 -3.38 -2.60 -5.44 -1.18 -2.99
Cell Periphery 4.28 -0.24 -2.45 -8.68 -3.02 -0.90 -5.49 -4.43 0.19 -1.41 0.43 -3.10 -4.74 -3.09 -2.68
Cytoplasm -5.01 8.92 8.70 38.89 -0.51 3.15 28.51 20.64 11.24 -0.06 -6.99 33.43 30.42 39.91 -1.60
Cytoplasmic Foci 5.80 8.67 8.49 17.06 -4.10 -1.02 23.80 16.70 8.08 -5.82 8.74 25.63 24.15 18.59 -5.95
Eisosomes 5.50 5.02 4.72 -4.72 -1.93 -1.70 2.97 -0.33 1.63 -3.04 6.93 8.39 7.25 -1.54 -1.92
Endoplasmic Reticulum 2.00 0.36 0.51 -8.21 0.69 -0.37 -1.81 -0.75 -0.26 -0.32 4.32 0.19 0.54 -3.98 0.52
Endosome 3.10 3.68 3.35 1.22 -1.57 -2.39 5.24 0.09 2.06 -1.47 2.96 7.88 5.31 2.22 -2.00
Golgi -1.24 0.16 -2.42 -2.15 -2.42 -0.52 2.70 0.21 0.58 -1.14 1.19 2.49 -1.43 -2.01 -2.54
Lipid Particles 2.85 0.91 -1.01 -8.72 -6.41 -0.78 -5.68 -6.20 -0.88 -3.57 1.83 -2.21 -6.67 -6.18 -6.42
Mitochondria -2.10 -2.35 -3.54 1.15 -0.46 -0.17 1.69 1.28 0.87 -0.03 -0.25 1.52 1.30 1.32 -0.51
Mitotic Spindle -3.85 -9.77 -11.77 -8.55 -3.24 -1.74 -5.07 -4.86 -2.13 -1.23 -2.72 -9.45 -11.44 -8.82 -3.50
None -2.40 -7.93 -4.63 -3.01 6.71 1.79 -3.31 -0.48 -3.13 3.40 -0.70 -5.37 -2.47 -3.48 7.18
Nuclear Periphery 2.29 -23.25 -22.55 -26.81 0.47 -0.55 -17.27 -9.59 -8.91 1.14 4.44 -30.61 -26.99 -28.28 0.93
Nuclear Periphery Foci -0.06 -0.21 -1.58 -1.81 -1.70 -0.98 -1.72 -3.59 -2.31 -2.62 1.28 0.66 -1.70 -2.33 -2.02
Nucleolus 1.54 1.19 0.71 -2.60 -1.53 -1.07 0.50 1.05 1.24 3.84 -0.07 0.59 0.36 0.56 -1.18
Nucleus 0.50 -24.04 -16.03 -29.83 11.47 2.88 -19.84 -4.64 -5.70 12.22 0.66 -45.14 -28.79 -29.71 13.95
Peroxisomes 0.34 5.30 3.70 2.85 -2.35 -1.93 2.46 1.99 2.31 -1.63 -1.22 2.92 2.04 3.66 -2.44
Vacuole 1.48 -5.12 -9.42 -21.63 -7.64 -3.15 -11.65 -16.51 -12.31 -7.03 6.13 -11.44 -18.57 -24.02 -7.98
Vacuole Periphery -1.51 2.32 -0.84 1.00 -1.92 0.23 1.20 1.95 1.11 0.51 0.60 2.29 1.69 1.22 -1.32
Standard name
Human Ortholog
Description Ubiquitin-like protein involved in thiolation of cytoplasmic tRNAs; receives sulfur from the E1-like enzyme Uba4p and transfers it to tRNA; also functions as a protein tag with roles in nutrient sensing and oxidative stress response
Localization
Cell Percentages cytoplasm (99%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Urm1

Urm1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Urm1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available