Standard name
Human Ortholog
Description Essential component of the TIM23 complex; tethers the import motor and regulatory factors (PAM complex) to the translocation channel (Tim23p-Tim17p core complex); TIM23 complex is short for the translocase of the inner mitochondrial membrane
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – 0.17 0.1 0 0.05 0 0 0 0.05
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – 0 0.1 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – 0.09 0.05 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – 0.09 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0.05 0 0 0 0 0 – 0 0 0 0 0 0.44 0.41 0.5 – 0.43 0.48 0.06 0.06 0 0.06 0.1 0.11
Mitochondria 0.88 0.97 0.96 0.96 0.93 0.92 – 0.89 0.93 0.94 0.94 0.91 0.74 0.75 0.61 – 0 0.05 0.85 0.83 0.83 0.78 0.75 0.71
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0.05 0.05 0.06 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0.13 0.07 0.1 0.08 0.12 0.15 – 0.2 0.17 0.15 0.15 0.14 0 0 0 – 0.15 0.14 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.09 0 0.06 0 0.06 0 – 0 0 0 0 0.05 0.19 0.18 0.27 – 0.15 0.24 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 2 0 0 0 0 – 0 0 0 1 0 9 7 10 – 8 2 3 5 12 6 16 20
Bud 2 2 3 1 3 1 – 6 5 10 4 5 1 1 1 – 0 2 0 0 1 0 0 1
Bud Neck 0 0 0 0 0 0 – 0 0 1 0 0 0 0 0 – 0 0 1 0 1 0 0 1
Bud Site 0 0 0 0 0 1 – 2 2 0 5 4 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 2 2 1 1 0 – 1 0 0 1 2 8 8 7 – 4 1 0 0 0 0 0 1
Cytoplasm 2 0 3 4 4 8 – 4 5 3 3 4 4 4 1 – 4 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 1 1 0 0 0 – 1 0 0 3 0 0 1 2 – 2 1 0 0 0 0 0 1
Endosome 1 0 0 1 2 0 – 0 0 0 0 0 0 0 0 – 0 0 1 1 3 2 4 2
Golgi 11 0 3 7 7 0 – 2 0 0 2 1 108 100 117 – 20 10 13 6 15 10 37 41
Mitochondria 181 101 326 265 334 361 – 603 371 471 379 411 184 184 143 – 2 1 187 89 296 133 282 274
Nucleus 0 0 1 1 0 4 – 0 1 1 1 1 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 4 2 2 1 5 10 – 18 22 25 24 8 5 5 3 – 0 0 0 0 2 0 1 5
Nucleolus 26 7 33 23 42 57 – 134 70 76 61 62 7 7 5 – 7 3 3 1 3 5 7 9
Peroxisomes 2 0 7 3 7 4 – 7 3 2 5 6 3 2 2 – 1 0 5 0 14 7 13 14
SpindlePole 0 0 0 1 1 0 – 0 2 2 3 3 0 0 0 – 0 0 0 0 0 1 1 0
Vac/Vac Membrane 19 1 21 5 23 12 – 23 9 9 4 21 47 45 64 – 7 5 2 3 2 1 13 13
Unique Cell Count 206 104 340 275 359 393 679 401 499 405 450 247 245 233 47 21 221 108 357 171 379 390
Labelled Cell Count 250 118 402 313 429 458 801 490 600 496 528 376 364 355 55 25 221 108 357 171 379 390


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 29.1 21.2 31.1 29.9 28.3 23.6 – 22.0 20.8 20.6 19.0 21.5 24.3 24.3 24.6 – 27.2 33.0 30.1 32.8 35.7
Std Deviation (1e-4) 9.4 6.6 10.4 9.7 10.0 8.6 – 7.6 7.9 7.8 7.9 9.3 7.4 7.4 6.3 – 13.0 14.4 9.7 10.6 12.4
Intensity Change (Log2) – – – -0.05 -0.14 -0.4 – -0.5 -0.58 -0.6 -0.71 -0.53 -0.36 -0.36 -0.34 – -0.19 0.09 -0.05 0.08 0.2


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 – 0 0
Bud 0 0 0 – 0 0 1.3 0 0 0 0 0 – 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 – 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 – 0 0
Cell Periphery 0 0 0 – 0 0 0 0 0 0 0 0 – 0 0
Cytoplasm 0 0 1.3 – 0 0 0 0 0 0 0 0 – 0 0
Endoplasmic Reticulum 0 0 0 – 0 0 0 0 0 0 0 0 – 0 0
Endosome 0 0 0 – 0 0 0 0 0 0 0 0 – 0 0
Golgi 0 0 0 – 0 0 0 0 0 13.1 12.5 14.3 – 0 0
Mitochondria 0.3 -1.6 -2.2 – -3.8 -1.9 -1.0 -1.4 -2.5 -7.6 -7.4 -10.5 – -16.4 -13.9
Nucleus 0 0 0 – 0 0 0 0 0 0 0 0 – 0 0
Nuclear Periphery 0 0 2.1 – 2.2 3.8 3.6 4.0 0 0 0 0 – 0 0
Nucleolus -0.6 0.9 2.0 – 4.1 3.0 2.3 2.2 1.7 -3.3 -3.2 -3.6 – 0 0
Peroxisomes 0 -0.1 -1.2 – 0 0 0 -0.9 -0.8 0 0 0 – 0 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 – 0 0
Vacuole -2.7 0.1 -2.0 – -2.1 -2.7 -3.3 -3.9 -0.9 4.8 4.6 7.0 – 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 22.167 25.0448 21.8012 20.5787 20.8035 22.8188 26.8409 25.3988 24.7655 26.4193 22.7025 25.2962 25.6197 25.8582 25.9039 26.1287 24.8368 26.6705
Actin 0.0131 0.003 0.0078 0.0233 0.0256 0.0001 0.0105 0.0021 0.0108 0 0.0036 0.0006 0.0076 0.0024 0.0042 0 0.0036 0.0002
Bud 0.0013 0.0006 0.0006 0.0054 0.0005 0 0.0007 0.0001 0.001 0 0.0005 0 0.0002 0.0007 0.0001 0 0.0001 0
Bud Neck 0.0012 0.0001 0.0011 0.0001 0.0002 0 0.0003 0.0001 0.0006 0 0.0002 0 0.0011 0.0002 0.0001 0 0.0001 0
Bud Periphery 0.0021 0.0007 0.0011 0.0133 0.0007 0 0.001 0.0002 0.0016 0 0.0008 0 0.0003 0.0007 0.0001 0 0.0001 0
Bud Site 0.0074 0.0009 0.0066 0.0051 0.0005 0 0.0018 0.0005 0.0025 0 0.0014 0 0.0014 0.0087 0.0004 0 0.0004 0
Cell Periphery 0.0009 0.0008 0.0006 0.0005 0.0004 0 0.001 0.0008 0.0007 0 0.0007 0 0.0004 0.0011 0.0003 0 0.0001 0.0001
Cytoplasm 0.0043 0.0001 0.0016 0.0003 0.0239 0 0.0005 0 0.0003 0 0.001 0 0.0004 0.0001 0 0 0.0267 0
Cytoplasmic Foci 0.0112 0.002 0.0121 0.0054 0.0225 0 0.0103 0.003 0.004 0 0.008 0.0018 0.0279 0.001 0.0013 0 0.0014 0.0001
Eisosomes 0.0006 0.0004 0.0002 0.0002 0.0004 0.0003 0.0003 0.0002 0.0013 0 0.0003 0.0001 0.0003 0.0002 0.0001 0 0.0001 0.0001
Endoplasmic Reticulum 0.002 0.0001 0.0009 0.0001 0.0078 0 0.0003 0.0001 0.0003 0 0.0008 0 0.0001 0 0 0 0.0043 0
Endosome 0.0135 0.0041 0.0065 0.0027 0.0131 0.0001 0.0029 0.0018 0.0009 0 0.0102 0.0004 0.0075 0.0012 0.0012 0 0.005 0.0001
Golgi 0.0042 0.0048 0.0021 0.0044 0.0127 0.0003 0.0019 0.0066 0.0013 0 0.0034 0.0036 0.0082 0.0019 0.0027 0 0.0004 0.0004
Lipid Particles 0.0146 0.012 0.0089 0.0179 0.016 0.0014 0.0191 0.0291 0.0089 0.0001 0.0191 0.0103 0.0427 0.0239 0.0041 0.0006 0.0019 0.0166
Mitochondria 0.8408 0.9145 0.9156 0.8722 0.8163 0.9966 0.8974 0.9164 0.9256 0.9992 0.8988 0.9726 0.8455 0.9189 0.9547 0.998 0.9378 0.9645
None 0.0036 0 0.0021 0.0003 0.0051 0 0.0022 0 0.0006 0 0.0039 0.0002 0.0003 0 0 0 0.0021 0
Nuclear Periphery 0.0129 0.0002 0.0034 0.0003 0.0164 0 0.0008 0.0012 0.0002 0 0.0077 0.0001 0.0002 0 0 0 0.0068 0.0002
Nucleolus 0.004 0.0025 0.0016 0.0003 0.0045 0 0.01 0.0049 0.0137 0 0.0083 0.0019 0.0077 0.0018 0.0008 0 0.0002 0.0008
Nucleus 0.0189 0.0005 0.002 0.0002 0.0142 0 0.0014 0.0004 0.001 0 0.0026 0.0002 0.0005 0.0003 0.0001 0 0.0043 0.0001
Peroxisomes 0.0148 0.0045 0.0047 0.0313 0.0019 0.0003 0.006 0.0057 0.0051 0.0002 0.0077 0.0009 0.0392 0.0055 0.0039 0.0001 0.0006 0.0006
Punctate Nuclear 0.0115 0 0.0057 0.0006 0.0023 0 0.0028 0 0.0015 0 0.0106 0.0006 0.0012 0 0 0 0.0026 0
Vacuole 0.0042 0.0133 0.0068 0.0025 0.0057 0.0001 0.0081 0.0051 0.0057 0.0001 0.0032 0.0003 0.0023 0.012 0.0047 0.0001 0.0005 0.0008
Vacuole Periphery 0.0128 0.0349 0.0081 0.0135 0.0092 0.0007 0.0208 0.022 0.0126 0.0004 0.0071 0.0063 0.0052 0.0194 0.021 0.0011 0.001 0.0154

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 41.7182 50.9932 45.1811 45.5881 68.4398 66.3718 53.0983 49.6015 67.2368 49.4738
Translational Efficiency 1.64 1.4464 1.5205 1.9815 1.0127 0.8686 1.1424 1.5409 1.1303 1.2583

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
256 1157 388 1115 1164 403 2711 1614 1420 1560 3099 2729

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 2043.34 2035.42 2270.58 2417.49 1560.98 2159.12 2226.66 2260.36 1647.94 2067.38 2232.16 2324.56
Standard Deviation 506.60 649.28 531.77 534.70 382.22 555.61 472.88 518.26 447.67 628.76 480.86 530.69
Intensity Change Log 2 — -0.005603 0.152132 0.242581 — 0.467991 0.512429 0.534101 — 0.218786 0.319313 0.376118

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.007327 0.014189 0.017919 0.017091 0.003235 0.005240 0.004507 0.007310 0.003973 0.011877 0.006186 0.011306
Bud Neck 0.014346 0.003255 0.007298 0.003668 0.001254 0.003689 0.001148 0.001655 0.003614 0.003367 0.001918 0.002478
Bud Site 0.026897 0.003215 0.001965 0.003318 0.000996 0.004262 0.001261 0.001837 0.005665 0.003486 0.001349 0.002442
Cell Periphery 0.000952 0.000322 0.000160 0.000261 0.000125 0.000173 0.000071 0.000201 0.000274 0.000283 0.000082 0.000225
Cytoplasm 0.001217 0.000343 0.000278 0.000308 0.000436 0.000515 0.000223 0.000207 0.000577 0.000387 0.000230 0.000248
Cytoplasmic Foci 0.036374 0.030862 0.043535 0.016462 0.012133 0.038858 0.011178 0.008759 0.016503 0.032928 0.015229 0.011906
Eisosomes 0.000973 0.004858 0.003360 0.001857 0.001451 0.001216 0.000849 0.001350 0.001365 0.003917 0.001163 0.001557
Endoplasmic Reticulum 0.000480 0.000307 0.001520 0.000337 0.000136 0.000227 0.000077 0.000126 0.000198 0.000286 0.000258 0.000212
Endosome 0.009135 0.001693 0.001798 0.002384 0.000422 0.003446 0.001029 0.001049 0.001993 0.002146 0.001125 0.001594
Golgi 0.032862 0.018912 0.040892 0.020872 0.006177 0.022860 0.018717 0.011796 0.010988 0.019932 0.021493 0.015504
Lipid Particles 0.011506 0.012466 0.019842 0.004003 0.003904 0.007011 0.008185 0.002830 0.005274 0.011057 0.009645 0.003309
Mitochondria 0.704695* 0.867504* 0.800552* 0.887828* 0.936242* 0.856044* 0.925261* 0.939313* 0.894499* 0.864543* 0.909648* 0.918278*
Mitotic Spindle 0.013241 0.000791 0.005131 0.003203 0.001008 0.002030 0.000765 0.000987 0.003213 0.001111 0.001311 0.001892
None 0.003009 0.004788 0.006954 0.003488 0.005634 0.004063 0.004399 0.002296 0.005161 0.004601 0.004719 0.002783
Nuclear Periphery 0.002424 0.000408 0.003005 0.000663 0.000705 0.000968 0.000906 0.000337 0.001015 0.000553 0.001169 0.000470
Nuclear Periphery Foci 0.001659 0.000350 0.001063 0.000231 0.000378 0.000911 0.000253 0.000196 0.000609 0.000494 0.000355 0.000210
Nucleolus 0.004920 0.002443 0.002092 0.000746 0.001339 0.001689 0.000808 0.000586 0.001985 0.002248 0.000969 0.000651
Nucleus 0.001734 0.000253 0.000138 0.000108 0.000932 0.000369 0.000042 0.000069 0.001076 0.000283 0.000054 0.000085
Peroxisomes 0.012687 0.014663 0.016833 0.008881 0.010205 0.010062 0.002470 0.006072 0.010652 0.013474 0.004268 0.007219
Vacuole 0.054649 0.008042 0.009123 0.009057 0.003409 0.017339 0.004660 0.004815 0.012646 0.010444 0.005219 0.006548
Vacuole Periphery 0.058912 0.010337 0.016542 0.015237 0.009881 0.019029 0.013191 0.008209 0.018720 0.012583 0.013611 0.011081

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.54 -3.21 -3.56 -0.99 0.23 -1.18 -1.21 -3.12 -1.16 -2.42 -4.52 -2.19 -5.64 0.30 -4.17
Bud Neck 4.62 2.44 4.40 -0.73 1.92 -2.30 0.14 -0.56 2.45 -1.91 0.35 2.23 1.47 1.78 -1.67
Bud Site 4.19 4.45 4.17 -0.22 -1.93 -2.11 -0.54 -1.30 1.55 -1.06 1.66 3.85 2.72 1.15 -2.50
Cell Periphery 2.36 3.09 2.69 0.65 -2.44 -1.26 2.46 -1.72 -0.39 -3.35 -0.12 3.83 0.86 0.85 -5.35
Cytoplasm 3.68 4.00 3.81 0.45 -0.64 -0.31 0.88 1.01 1.60 0.18 0.90 1.63 1.65 2.07 -0.21
Cytoplasmic Foci 1.01 -0.83 3.32 4.65 5.23 -5.04 0.58 1.97 5.76 1.90 -6.00 0.77 2.51 8.65 2.39
Eisosomes -6.63 -5.59 -4.09 5.11 3.51 1.36 4.95 0.70 -0.82 -4.64 -5.89 1.73 -1.52 5.47 -3.59
Endoplasmic Reticulum 1.80 -1.03 1.28 -0.38 1.17 -1.44 2.07 0.27 1.57 -1.59 -2.10 -0.46 -0.31 1.64 0.35
Endosome 5.04 4.98 4.36 -1.13 -0.97 -3.86 -4.17 -2.41 2.92 -0.11 -0.37 2.86 1.01 1.37 -1.64
Golgi 2.49 -1.13 2.08 -0.73 3.84 -4.05 -8.11 -3.80 2.61 3.80 -4.49 -5.94 -2.74 2.25 3.45
Lipid Particles -0.22 -1.55 1.96 5.03 4.16 -1.76 -4.36 1.19 2.40 5.78 -3.78 -3.69 2.00 5.88 7.12
Mitochondria -7.42 -3.90 -8.35 -2.07 -5.75 6.01 1.95 -0.50 -6.35 -2.81 3.51 -2.30 -3.48 -7.61 -1.72
Mitotic Spindle 2.83 1.80 2.26 -2.85 1.40 -0.84 0.39 0.04 0.95 -0.76 2.03 1.98 1.31 -1.43 -1.49
None -5.30 -5.71 -1.57 4.45 5.17 3.17 3.27 9.92 4.53 9.18 1.69 1.34 8.36 8.32 8.85
Nuclear Periphery 4.13 -0.57 3.60 -2.75 2.58 -0.47 -0.41 0.77 1.94 3.92 1.13 -0.36 1.36 0.76 4.19
Nuclear Periphery Foci 4.07 1.47 4.47 2.22 3.24 -1.57 0.80 1.10 2.24 0.84 0.59 1.74 2.73 2.94 2.55
Nucleolus 1.37 1.54 2.33 3.73 2.50 -0.78 1.33 2.04 2.91 0.98 -0.45 2.17 3.02 4.57 1.59
Nucleus 1.20 1.29 1.31 3.37 0.92 0.73 1.33 1.29 1.50 -1.92 1.32 1.72 1.67 3.08 -2.76
Peroxisomes -0.67 -1.00 1.35 2.55 2.16 0.04 4.48 2.08 1.84 -3.40 -1.43 4.14 2.02 3.92 -2.96
Vacuole 6.91 6.69 6.71 -0.63 0.01 -5.25 -1.81 -1.51 4.59 -0.20 1.29 5.15 3.92 3.06 -1.65
Vacuole Periphery 6.88 5.89 6.15 -2.40 0.53 -2.48 -1.96 0.98 3.07 3.89 2.76 2.59 3.87 0.96 2.16
Standard name
Human Ortholog
Description Essential component of the TIM23 complex; tethers the import motor and regulatory factors (PAM complex) to the translocation channel (Tim23p-Tim17p core complex); TIM23 complex is short for the translocase of the inner mitochondrial membrane
Localization
Cell Percentages mitochondrion (91%)
Cell Cycle Regulation No
Subcompartmental Group mito-3

Tim44

Tim44


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Tim44-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available