Standard name | |
---|---|
Human Ortholog | |
Description | Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response, and also shows K1 killer toxin resistance; homologous to worm B0334.15/EMC-5, fly CG15168, human MMGT |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 8 | 5 | 26 | 24 | 24 | 37 | – | 26 | 17 | 24 | 17 | 16 | 16 | 18 | 19 | 5 | 4 | 7 | 2 | 3 | 6 | 4 | 6 | 10 |
Bud | 1 | 0 | 6 | 2 | 2 | 3 | – | 7 | 2 | 6 | 6 | 8 | 1 | 1 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 4 | 9 | 14 | 18 | 11 | 27 | – | 18 | 24 | 42 | 31 | 35 | 40 | 73 | 81 | 31 | 21 | 24 | 0 | 2 | 2 | 0 | 6 | 4 |
Cytoplasm | 22 | 24 | 30 | 18 | 29 | 32 | – | 36 | 28 | 38 | 40 | 39 | 22 | 25 | 56 | 17 | 16 | 16 | 5 | 4 | 3 | 8 | 4 | 5 |
Endoplasmic Reticulum | 245 | 273 | 218 | 195 | 188 | 274 | – | 257 | 191 | 219 | 155 | 141 | 289 | 324 | 363 | 2 | 1 | 0 | 275 | 290 | 296 | 166 | 274 | 323 |
Endosome | 0 | 0 | 1 | 0 | 1 | 2 | – | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 2 |
Golgi | 0 | 1 | 2 | 10 | 19 | 4 | – | 1 | 0 | 1 | 1 | 0 | 15 | 10 | 7 | 9 | 19 | 14 | 0 | 2 | 1 | 2 | 5 | 4 |
Mitochondria | 0 | 0 | 59 | 97 | 54 | 121 | – | 151 | 137 | 119 | 107 | 136 | 1 | 3 | 2 | 1 | 11 | 13 | 0 | 0 | 5 | 2 | 3 | 3 |
Nucleus | 0 | 0 | 1 | 0 | 0 | 0 | – | 1 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 2 | 3 | 8 | 7 | 3 | 23 | – | 26 | 28 | 26 | 10 | 12 | 9 | 5 | 4 | 3 | 1 | 1 | 2 | 0 | 1 | 1 | 1 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 20 | 7 | 32 | 1 | 18 | 18 | – | 41 | 29 | 19 | 10 | 24 | 2 | 1 | 22 | 1 | 4 | 4 | 4 | 3 | 3 | 2 | 7 | 11 |
Unique Cell Count | 278 | 297 | 288 | 246 | 262 | 383 | 427 | 329 | 364 | 275 | 303 | 327 | 382 | 451 | 59 | 63 | 67 | 297 | 310 | 326 | 195 | 314 | 374 | |
Labelled Cell Count | 302 | 322 | 397 | 372 | 349 | 541 | 568 | 457 | 494 | 378 | 412 | 396 | 461 | 555 | 71 | 77 | 80 | 297 | 310 | 326 | 195 | 314 | 374 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 16.7 | 20.4 | 10.3 | 12.6 | 12.3 | 10.1 | – | 9.5 | 8.6 | 9.0 | 8.7 | 8.8 | 16.5 | 16.6 | 15.8 | 24.5 | 23.6 | 22.1 | 17.0 | 18.3 | 18.3 |
Std Deviation (1e-4) | 2.6 | 3.4 | 2.3 | 2.1 | 2.1 | 1.8 | – | 1.9 | 1.5 | 1.5 | 1.4 | 1.8 | 2.8 | 2.7 | 2.4 | 5.3 | 5.6 | 4.9 | 3.6 | 3.2 | 3.5 |
Intensity Change (Log2) | – | – | – | 0.3 | 0.27 | -0.02 | – | -0.11 | -0.25 | -0.19 | -0.23 | -0.22 | 0.68 | 0.69 | 0.62 | 1.26 | 1.2 | 1.11 | 0.73 | 0.84 | 0.83 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0.3 | 0.1 | 0.3 | – | -1.5 | -1.9 | -1.2 | -1.3 | -1.8 | -2.0 | -2.2 | -2.7 | -0.1 | -0.7 | 0.4 |
Bud | 0 | 0 | 0 | – | -0.4 | 0 | -0.4 | 0.1 | 0.4 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 1.2 | -0.4 | 1.2 | – | -0.4 | 1.3 | 3.0 | 2.8 | 2.9 | 3.2 | 5.4 | 5.2 | 9.9 | 6.8 | 7.4 |
Cytoplasm | -1.2 | 0.2 | -0.9 | – | -0.9 | -0.8 | 0 | 1.5 | 0.9 | -1.6 | -1.8 | 0.8 | 3.8 | 3.2 | 3.0 |
Endoplasmic Reticulum | 1.0 | -1.0 | -1.2 | – | -4.3 | -4.6 | -4.2 | -4.8 | -7.3 | 4.1 | 3.0 | 1.5 | -10.5 | -11.0 | -11.5 |
Endosome | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 2.6 | 4.0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 2.9 | 1.9 | 0 | 0 | 0 | 0 |
Mitochondria | 4.8 | 0 | 3.2 | – | 4.3 | 5.6 | 3.5 | 4.8 | 6.3 | -8.4 | -8.7 | -9.7 | -3.5 | -0.5 | -0.2 |
Nucleus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | -1.4 | 2.0 | – | 2.0 | 3.0 | 2.5 | 0.6 | 0.8 | 0 | -1.4 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -5.1 | -1.7 | -3.1 | – | -0.7 | -1.0 | -2.8 | -3.4 | -1.3 | -5.7 | -6.4 | -3.2 | -2.2 | -1.1 | -1.3 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 11.2538 | 12.4692 | 11.3873 | 9.6794 | 9.3328 | 11.9163 | 10.6215 | 11.7651 | 10.7328 | 10.6519 | 9.7779 | 11.4231 | 14.8625 | 14.943 | 14.262 | 13.201 | 12.657 | 13.7126 |
Actin | 0.0101 | 0.0003 | 0.0099 | 0.0213 | 0.012 | 0.0046 | 0.0155 | 0.0022 | 0.0095 | 0.0005 | 0.0114 | 0.0004 | 0.024 | 0.0063 | 0.0144 | 0.023 | 0.0018 | 0.0061 |
Bud | 0.0003 | 0 | 0.0003 | 0.0024 | 0.0004 | 0.0006 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0 | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0003 |
Bud Neck | 0.0011 | 0.0003 | 0.0006 | 0.0011 | 0.0023 | 0.0004 | 0.0011 | 0.0023 | 0.0016 | 0.0005 | 0.0005 | 0.0006 | 0.0007 | 0.0004 | 0.0024 | 0.0004 | 0.0003 | 0.0014 |
Bud Periphery | 0.0006 | 0.0001 | 0.0005 | 0.0023 | 0.0012 | 0.0003 | 0.0004 | 0.0003 | 0.0003 | 0.0008 | 0.0002 | 0.0001 | 0.0008 | 0.0003 | 0.0003 | 0.0003 | 0.0003 | 0.0007 |
Bud Site | 0.0032 | 0.0003 | 0.001 | 0.013 | 0.0006 | 0.0016 | 0.0027 | 0.0067 | 0.0027 | 0.0003 | 0.0006 | 0 | 0.0022 | 0.0005 | 0.0008 | 0.0003 | 0.0006 | 0.0012 |
Cell Periphery | 0.0011 | 0.0056 | 0.0018 | 0.0026 | 0.002 | 0.0003 | 0.0022 | 0.0077 | 0.0056 | 0.0021 | 0.0009 | 0.0012 | 0.0007 | 0.0069 | 0.0085 | 0.0005 | 0.0014 | 0.0011 |
Cytoplasm | 0.0058 | 0.012 | 0.0034 | 0.0139 | 0.003 | 0.0085 | 0.0069 | 0.0269 | 0.0059 | 0.0051 | 0.0072 | 0.008 | 0.0195 | 0.0404 | 0.0114 | 0.0017 | 0.0034 | 0.0147 |
Cytoplasmic Foci | 0.0232 | 0.0006 | 0.0069 | 0.0175 | 0.0201 | 0.0014 | 0.013 | 0.0038 | 0.0075 | 0.0033 | 0.0097 | 0.0017 | 0.0054 | 0.0016 | 0.0046 | 0.0011 | 0.0066 | 0.0014 |
Eisosomes | 0.0003 | 0 | 0.0001 | 0.0005 | 0.0001 | 0 | 0.0002 | 0.0001 | 0.0002 | 0 | 0.0001 | 0 | 0.0005 | 0.0006 | 0.0002 | 0.0009 | 0.0001 | 0.0001 |
Endoplasmic Reticulum | 0.8133 | 0.9609 | 0.9146 | 0.7608 | 0.7899 | 0.9612 | 0.817 | 0.8852 | 0.9008 | 0.923 | 0.8602 | 0.9579 | 0.8405 | 0.8989 | 0.9202 | 0.895 | 0.9069 | 0.9323 |
Endosome | 0.0195 | 0.0025 | 0.0125 | 0.0353 | 0.044 | 0.0046 | 0.0353 | 0.0117 | 0.0201 | 0.0081 | 0.0586 | 0.0062 | 0.0137 | 0.0046 | 0.0067 | 0.0117 | 0.0133 | 0.0072 |
Golgi | 0.0053 | 0.0026 | 0.0074 | 0.0161 | 0.0536 | 0.0017 | 0.0129 | 0.0083 | 0.0092 | 0.0051 | 0.0209 | 0.0031 | 0.004 | 0.0071 | 0.0032 | 0.0097 | 0.0043 | 0.0055 |
Lipid Particles | 0.0091 | 0.006 | 0.0073 | 0.0282 | 0.018 | 0.001 | 0.0168 | 0.0288 | 0.0115 | 0.0201 | 0.0092 | 0.0067 | 0.0032 | 0.0163 | 0.0057 | 0.0105 | 0.0212 | 0.003 |
Mitochondria | 0.0076 | 0.0001 | 0.0131 | 0.0569 | 0.0071 | 0.0009 | 0.0067 | 0.0016 | 0.0016 | 0.0025 | 0.0031 | 0.0011 | 0.0064 | 0.0028 | 0.0048 | 0.0105 | 0.0033 | 0.0017 |
None | 0.0027 | 0 | 0.0002 | 0.0006 | 0.0002 | 0.0008 | 0.001 | 0.0001 | 0.0016 | 0.0001 | 0.0004 | 0.0001 | 0.0024 | 0.0002 | 0.0008 | 0.0009 | 0.0008 | 0.0002 |
Nuclear Periphery | 0.0651 | 0.0057 | 0.0077 | 0.0035 | 0.0079 | 0.0097 | 0.0452 | 0.0069 | 0.0072 | 0.0063 | 0.0053 | 0.0077 | 0.0427 | 0.0093 | 0.0074 | 0.0252 | 0.0054 | 0.0174 |
Nucleolus | 0.0018 | 0 | 0.0001 | 0.0007 | 0 | 0 | 0.0001 | 0.0001 | 0.0009 | 0.0002 | 0.0001 | 0 | 0.0004 | 0 | 0.0001 | 0.0002 | 0.0005 | 0.0001 |
Nucleus | 0.0045 | 0.0003 | 0.0004 | 0.0007 | 0.0004 | 0.0005 | 0.0026 | 0.0007 | 0.0012 | 0.001 | 0.0008 | 0.0015 | 0.0241 | 0.0007 | 0.0007 | 0.0017 | 0.0004 | 0.002 |
Peroxisomes | 0.0131 | 0 | 0.0067 | 0.0122 | 0.031 | 0.0002 | 0.0097 | 0.0002 | 0.0038 | 0.0029 | 0.0015 | 0.0012 | 0.0012 | 0 | 0.0005 | 0.002 | 0.0202 | 0.0005 |
Punctate Nuclear | 0.0023 | 0.0001 | 0.0003 | 0.0007 | 0.0005 | 0.0003 | 0.0012 | 0.0002 | 0.0016 | 0.0001 | 0.0012 | 0.0003 | 0.0032 | 0.0001 | 0.0028 | 0.0011 | 0.0061 | 0.0004 |
Vacuole | 0.0048 | 0.0024 | 0.0041 | 0.0077 | 0.0044 | 0.0009 | 0.0062 | 0.0055 | 0.0065 | 0.0156 | 0.0064 | 0.0018 | 0.0024 | 0.0024 | 0.0034 | 0.0015 | 0.0022 | 0.0023 |
Vacuole Periphery | 0.0053 | 0.0002 | 0.0013 | 0.0019 | 0.0015 | 0.0004 | 0.0032 | 0.0007 | 0.0006 | 0.0022 | 0.0016 | 0.0004 | 0.0016 | 0.0005 | 0.001 | 0.0018 | 0.0008 | 0.0006 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 27.1966 | 34.7316 | 29.2086 | 45.1442 | 50.3737 | 25.8872 | 39.7993 | 34.7624 | 30.1053 | 50.8133 |
Translational Efficiency | 2.0758 | 1.6722 | 1.8922 | 0.9675 | 1.1419 | 2.1836 | 1.3731 | 1.6318 | 1.7763 | 1.5839 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1664 | 1550 | 420 | 1761 | 593 | 1906 | 2429 | 1386 | 2257 | 3456 | 2849 | 3147 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1306.00 | 1492.21 | 1923.81 | 1858.11 | 1043.29 | 1404.88 | 1902.19 | 1954.98 | 1236.98 | 1444.05 | 1905.38 | 1900.77 |
Standard Deviation | 194.38 | 253.46 | 271.92 | 440.01 | 195.85 | 243.05 | 264.34 | 372.35 | 226.50 | 251.55 | 265.58 | 414.38 |
Intensity Change Log 2 | — | 0.192296 | 0.558811 | 0.508681 | — | 0.429307 | 0.866521 | 0.906014 | — | 0.302380 | 0.703612 | 0.698736 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.001078 | 0.000573 | 0.000126 | 0.004616 | 0.003517 | 0.001740 | 0.000936 | 0.004345 | 0.001719 | 0.001217 | 0.000817 | 0.004497 |
Bud Neck | 0.000289 | 0.000094 | 0.001271 | 0.004867 | 0.000326 | 0.000115 | 0.000410 | 0.003647 | 0.000298 | 0.000106 | 0.000537 | 0.004330 |
Bud Site | 0.002104 | 0.001043 | 0.001191 | 0.031505 | 0.007746 | 0.002707 | 0.001054 | 0.025093 | 0.003587 | 0.001960 | 0.001075 | 0.028681 |
Cell Periphery | 0.002620 | 0.002147 | 0.003630 | 0.015958 | 0.007358 | 0.002452 | 0.003816 | 0.030079 | 0.003865 | 0.002315 | 0.003789 | 0.022177 |
Cytoplasm | 0.002402 | 0.002438 | 0.001012 | 0.022264 | 0.006211 | 0.003993 | 0.001468 | 0.010282 | 0.003403 | 0.003296 | 0.001401 | 0.016987 |
Cytoplasmic Foci | 0.003124 | 0.001776 | 0.000112 | 0.013748 | 0.008941 | 0.003529 | 0.000186 | 0.008071 | 0.004652 | 0.002743 | 0.000175 | 0.011248 |
Eisosomes | 0.000116 | 0.000088 | 0.000059 | 0.000268 | 0.000208 | 0.000128 | 0.000113 | 0.000478 | 0.000140 | 0.000110 | 0.000105 | 0.000361 |
Endoplasmic Reticulum | 0.971646* | 0.973656* | 0.950863* | 0.721732* | 0.918258* | 0.965737* | 0.979249* | 0.738532* | 0.957619* | 0.969288* | 0.975065* | 0.729131* |
Endosome | 0.004937 | 0.005290 | 0.007266 | 0.025039 | 0.009015 | 0.005310 | 0.002761 | 0.023195 | 0.006009 | 0.005301 | 0.003425 | 0.024227 |
Golgi | 0.003004 | 0.002502 | 0.002450 | 0.018418 | 0.007449 | 0.003706 | 0.001105 | 0.022503 | 0.004172 | 0.003166 | 0.001303 | 0.020217 |
Lipid Particles | 0.003523 | 0.003853 | 0.004051 | 0.081830 | 0.002481 | 0.004207 | 0.003504 | 0.094122 | 0.003249 | 0.004048 | 0.003585 | 0.087244 |
Mitochondria | 0.000895 | 0.000688 | 0.000184 | 0.002794 | 0.008517 | 0.001369 | 0.000157 | 0.002511 | 0.002897 | 0.001064 | 0.000161 | 0.002669 |
Mitotic Spindle | 0.000314 | 0.000663 | 0.000079 | 0.005938 | 0.000275 | 0.000092 | 0.000155 | 0.003048 | 0.000304 | 0.000348 | 0.000144 | 0.004665 |
None | 0.000886 | 0.001545 | 0.000080 | 0.001054 | 0.001098 | 0.001061 | 0.001081 | 0.001563 | 0.000942 | 0.001278 | 0.000933 | 0.001278 |
Nuclear Periphery | 0.000204 | 0.000245 | 0.003408 | 0.001269 | 0.000797 | 0.000113 | 0.000331 | 0.001146 | 0.000360 | 0.000172 | 0.000784 | 0.001215 |
Nuclear Periphery Foci | 0.001220 | 0.001158 | 0.014057 | 0.017304 | 0.002284 | 0.001247 | 0.001241 | 0.007176 | 0.001500 | 0.001207 | 0.003131 | 0.012843 |
Nucleolus | 0.000008 | 0.000002 | 0.000006 | 0.000051 | 0.000335 | 0.000005 | 0.000002 | 0.000030 | 0.000094 | 0.000004 | 0.000002 | 0.000042 |
Nucleus | 0.000125 | 0.000050 | 0.000085 | 0.001242 | 0.007196 | 0.000090 | 0.000070 | 0.000533 | 0.001983 | 0.000072 | 0.000072 | 0.000930 |
Peroxisomes | 0.000594 | 0.000356 | 0.000006 | 0.000824 | 0.001352 | 0.000674 | 0.000281 | 0.001005 | 0.000793 | 0.000531 | 0.000240 | 0.000904 |
Vacuole | 0.000521 | 0.001321 | 0.008302 | 0.023609 | 0.003748 | 0.001113 | 0.001907 | 0.018160 | 0.001369 | 0.001206 | 0.002850 | 0.021210 |
Vacuole Periphery | 0.000388 | 0.000512 | 0.001761 | 0.005669 | 0.002888 | 0.000614 | 0.000174 | 0.004482 | 0.001045 | 0.000568 | 0.000408 | 0.005147 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 1.10 | 2.48 | -4.78 | -5.81 | -6.85 | 1.47 | 2.17 | -0.82 | -3.65 | -5.09 | 1.00 | 1.65 | -4.87 | -6.34 | -6.79 |
Bud Neck | 1.40 | -3.48 | -9.25 | -9.92 | -6.77 | 3.36 | -0.69 | -7.27 | -7.74 | -6.85 | 1.86 | -1.64 | -11.60 | -12.57 | -10.75 |
Bud Site | 1.63 | 0.85 | -11.91 | -12.59 | -12.12 | 2.44 | 3.31 | -5.67 | -8.69 | -9.46 | 2.08 | 3.25 | -13.57 | -15.03 | -15.59 |
Cell Periphery | 0.95 | -1.83 | -10.31 | -10.85 | -9.42 | 3.06 | 2.16 | -8.36 | -12.36 | -11.65 | 2.83 | 0.09 | -14.05 | -16.27 | -14.65 |
Cytoplasm | -0.12 | 1.38 | -11.67 | -11.29 | -11.44 | 1.27 | 2.72 | -1.96 | -4.15 | -5.95 | 0.17 | 3.39 | -10.97 | -11.41 | -13.32 |
Cytoplasmic Foci | 1.53 | 3.71 | -7.71 | -10.18 | -12.07 | 3.04 | 5.26 | 0.43 | -4.25 | -8.96 | 2.28 | 6.01 | -6.37 | -10.27 | -14.87 |
Eisosomes | 0.84 | 1.71 | -4.82 | -5.12 | -6.00 | 0.79 | 0.94 | -2.41 | -5.78 | -6.13 | 0.86 | 0.96 | -5.42 | -7.55 | -7.43 |
Endoplasmic Reticulum | -0.52 | 3.32 | 29.77 | 30.01* | 22.63 | -5.32 | -7.01 | 14.94 | 24.64 | 26.85* | -3.30 | -4.91 | 34.76 | 38.57* | 39.76* |
Endosome | -0.54 | -2.82 | -17.62 | -16.20 | -13.31 | 3.55 | 6.34 | -8.50 | -12.39 | -14.51 | 1.34 | 5.77 | -19.51 | -20.53 | -23.42 |
Golgi | 0.66 | 0.66 | -10.20 | -11.20 | -10.97 | 2.27 | 4.12 | -6.20 | -9.37 | -11.15 | 1.36 | 4.41 | -12.42 | -14.20 | -16.39 |
Lipid Particles | -0.37 | -0.36 | -18.72 | -18.42 | -17.30 | -2.29 | -1.73 | -18.65 | -18.21 | -18.42 | -1.32 | -0.68 | -26.30 | -25.90 | -26.06 |
Mitochondria | 0.43 | 2.14 | -3.60 | -4.04 | -6.22 | 2.62 | 3.10 | 2.18 | -1.47 | -3.69 | 2.28 | 3.63 | 0.26 | -3.52 | -6.86 |
Mitotic Spindle | -0.61 | 1.22 | -5.30 | -4.54 | -5.60 | 1.18 | 0.56 | -3.16 | -3.40 | -3.29 | -0.16 | 0.86 | -6.12 | -5.87 | -6.39 |
None | -1.01 | 1.91 | -0.41 | 0.88 | -5.10 | 0.07 | 0.05 | -0.48 | -0.58 | -0.61 | -0.71 | 0.03 | -0.70 | -0.02 | -0.89 |
Nuclear Periphery | -0.54 | -6.57 | -8.62 | -7.47 | 4.21 | 1.10 | 0.74 | -0.58 | -7.11 | -5.39 | 1.11 | -2.35 | -4.65 | -10.70 | -3.57 |
Nuclear Periphery Foci | 0.16 | -4.33 | -11.25 | -11.14 | -1.07 | 1.24 | 1.16 | -4.34 | -6.57 | -6.35 | 0.92 | -2.91 | -12.42 | -12.84 | -9.44 |
Nucleolus | 1.22 | 0.25 | -4.47 | -5.50 | -4.99 | 1.80 | 1.81 | 1.66 | -2.94 | -3.27 | 1.86 | 1.88 | 1.03 | -6.14 | -6.29 |
Nucleus | 1.37 | 0.51 | -4.30 | -4.57 | -4.43 | 2.76 | 2.76 | 2.53 | -2.83 | -2.65 | 2.81 | 2.76 | 1.12 | -5.31 | -5.03 |
Peroxisomes | 1.83 | 5.06 | -1.38 | -3.65 | -7.27 | 1.91 | 3.15 | 0.95 | -1.38 | -3.41 | 1.78 | 3.73 | -0.65 | -2.76 | -4.90 |
Vacuole | -2.39 | -6.08 | -13.85 | -13.18 | -7.66 | 1.98 | 1.22 | -7.03 | -10.33 | -9.53 | 0.37 | -2.91 | -16.23 | -16.79 | -14.70 |
Vacuole Periphery | -0.73 | -2.72 | -7.41 | -7.11 | -4.52 | 3.49 | 4.20 | -1.76 | -5.96 | -6.71 | 2.33 | 3.16 | -7.92 | -9.24 | -9.58 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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