Standard name
Human Ortholog
Description Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response, and also shows K1 killer toxin resistance; homologous to worm B0334.15/EMC-5, fly CG15168, human MMGT
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0.09 0.1 0.09 0.1 – 0.06 0.05 0.07 0.06 0.05 0.05 0.05 0 0.08 0.06 0.1 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0.05 0.07 0 0.07 – 0 0.07 0.12 0.11 0.12 0.12 0.19 0.18 0.53 0.33 0.36 0 0 0 0 0 0
Cytoplasm 0.08 0.08 0.1 0.07 0.11 0.08 – 0.08 0.09 0.1 0.15 0.13 0.07 0.07 0.12 0.29 0.25 0.24 0 0 0 0 0 0
Endoplasmic Reticulum 0.88 0.92 0.76 0.79 0.72 0.72 – 0.6 0.58 0.6 0.56 0.47 0.88 0.85 0.8 0 0 0 0.93 0.94 0.91 0.85 0.87 0.87
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0.07 0 – 0 0 0 0 0 0.05 0 0 0.15 0.3 0.21 0 0 0 0 0 0
Mitochondria 0 0 0.2 0.39 0.21 0.32 – 0.35 0.42 0.33 0.39 0.45 0 0 0 0 0.17 0.19 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0.06 – 0.06 0.09 0.07 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.07 0 0.11 0 0.07 0.05 – 0.1 0.09 0.05 0 0.08 0 0 0.05 0 0.06 0.06 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 8 5 26 24 24 37 – 26 17 24 17 16 16 18 19 5 4 7 2 3 6 4 6 10
Bud 1 0 6 2 2 3 – 7 2 6 6 8 1 1 1 1 0 0 1 0 0 0 0 2
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 4 9 14 18 11 27 – 18 24 42 31 35 40 73 81 31 21 24 0 2 2 0 6 4
Cytoplasm 22 24 30 18 29 32 – 36 28 38 40 39 22 25 56 17 16 16 5 4 3 8 4 5
Endoplasmic Reticulum 245 273 218 195 188 274 – 257 191 219 155 141 289 324 363 2 1 0 275 290 296 166 274 323
Endosome 0 0 1 0 1 2 – 3 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 2
Golgi 0 1 2 10 19 4 – 1 0 1 1 0 15 10 7 9 19 14 0 2 1 2 5 4
Mitochondria 0 0 59 97 54 121 – 151 137 119 107 136 1 3 2 1 11 13 0 0 5 2 3 3
Nucleus 0 0 1 0 0 0 – 1 0 0 1 1 1 0 0 1 0 0 0 0 0 0 0 0
Nuclear Periphery 2 3 8 7 3 23 – 26 28 26 10 12 9 5 4 3 1 1 2 0 1 1 1 0
Nucleolus 0 0 0 0 0 0 – 0 1 0 0 0 0 1 0 0 0 1 0 0 0 2 0 0
Peroxisomes 0 0 0 0 0 0 – 1 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 20 7 32 1 18 18 – 41 29 19 10 24 2 1 22 1 4 4 4 3 3 2 7 11
Unique Cell Count 278 297 288 246 262 383 427 329 364 275 303 327 382 451 59 63 67 297 310 326 195 314 374
Labelled Cell Count 302 322 397 372 349 541 568 457 494 378 412 396 461 555 71 77 80 297 310 326 195 314 374


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 16.7 20.4 10.3 12.6 12.3 10.1 – 9.5 8.6 9.0 8.7 8.8 16.5 16.6 15.8 24.5 23.6 22.1 17.0 18.3 18.3
Std Deviation (1e-4) 2.6 3.4 2.3 2.1 2.1 1.8 – 1.9 1.5 1.5 1.4 1.8 2.8 2.7 2.4 5.3 5.6 4.9 3.6 3.2 3.5
Intensity Change (Log2) – – – 0.3 0.27 -0.02 – -0.11 -0.25 -0.19 -0.23 -0.22 0.68 0.69 0.62 1.26 1.2 1.11 0.73 0.84 0.83


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0.3 0.1 0.3 – -1.5 -1.9 -1.2 -1.3 -1.8 -2.0 -2.2 -2.7 -0.1 -0.7 0.4
Bud 0 0 0 – -0.4 0 -0.4 0.1 0.4 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 1.2 -0.4 1.2 – -0.4 1.3 3.0 2.8 2.9 3.2 5.4 5.2 9.9 6.8 7.4
Cytoplasm -1.2 0.2 -0.9 – -0.9 -0.8 0 1.5 0.9 -1.6 -1.8 0.8 3.8 3.2 3.0
Endoplasmic Reticulum 1.0 -1.0 -1.2 – -4.3 -4.6 -4.2 -4.8 -7.3 4.1 3.0 1.5 -10.5 -11.0 -11.5
Endosome 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 2.6 4.0 0 – 0 0 0 0 0 2.9 1.9 0 0 0 0
Mitochondria 4.8 0 3.2 – 4.3 5.6 3.5 4.8 6.3 -8.4 -8.7 -9.7 -3.5 -0.5 -0.2
Nucleus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 -1.4 2.0 – 2.0 3.0 2.5 0.6 0.8 0 -1.4 0 0 0 0
Nucleolus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vacuole -5.1 -1.7 -3.1 – -0.7 -1.0 -2.8 -3.4 -1.3 -5.7 -6.4 -3.2 -2.2 -1.1 -1.3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 11.2538 12.4692 11.3873 9.6794 9.3328 11.9163 10.6215 11.7651 10.7328 10.6519 9.7779 11.4231 14.8625 14.943 14.262 13.201 12.657 13.7126
Actin 0.0101 0.0003 0.0099 0.0213 0.012 0.0046 0.0155 0.0022 0.0095 0.0005 0.0114 0.0004 0.024 0.0063 0.0144 0.023 0.0018 0.0061
Bud 0.0003 0 0.0003 0.0024 0.0004 0.0006 0.0002 0.0001 0.0001 0.0002 0.0002 0 0.0004 0.0001 0.0001 0.0001 0.0001 0.0003
Bud Neck 0.0011 0.0003 0.0006 0.0011 0.0023 0.0004 0.0011 0.0023 0.0016 0.0005 0.0005 0.0006 0.0007 0.0004 0.0024 0.0004 0.0003 0.0014
Bud Periphery 0.0006 0.0001 0.0005 0.0023 0.0012 0.0003 0.0004 0.0003 0.0003 0.0008 0.0002 0.0001 0.0008 0.0003 0.0003 0.0003 0.0003 0.0007
Bud Site 0.0032 0.0003 0.001 0.013 0.0006 0.0016 0.0027 0.0067 0.0027 0.0003 0.0006 0 0.0022 0.0005 0.0008 0.0003 0.0006 0.0012
Cell Periphery 0.0011 0.0056 0.0018 0.0026 0.002 0.0003 0.0022 0.0077 0.0056 0.0021 0.0009 0.0012 0.0007 0.0069 0.0085 0.0005 0.0014 0.0011
Cytoplasm 0.0058 0.012 0.0034 0.0139 0.003 0.0085 0.0069 0.0269 0.0059 0.0051 0.0072 0.008 0.0195 0.0404 0.0114 0.0017 0.0034 0.0147
Cytoplasmic Foci 0.0232 0.0006 0.0069 0.0175 0.0201 0.0014 0.013 0.0038 0.0075 0.0033 0.0097 0.0017 0.0054 0.0016 0.0046 0.0011 0.0066 0.0014
Eisosomes 0.0003 0 0.0001 0.0005 0.0001 0 0.0002 0.0001 0.0002 0 0.0001 0 0.0005 0.0006 0.0002 0.0009 0.0001 0.0001
Endoplasmic Reticulum 0.8133 0.9609 0.9146 0.7608 0.7899 0.9612 0.817 0.8852 0.9008 0.923 0.8602 0.9579 0.8405 0.8989 0.9202 0.895 0.9069 0.9323
Endosome 0.0195 0.0025 0.0125 0.0353 0.044 0.0046 0.0353 0.0117 0.0201 0.0081 0.0586 0.0062 0.0137 0.0046 0.0067 0.0117 0.0133 0.0072
Golgi 0.0053 0.0026 0.0074 0.0161 0.0536 0.0017 0.0129 0.0083 0.0092 0.0051 0.0209 0.0031 0.004 0.0071 0.0032 0.0097 0.0043 0.0055
Lipid Particles 0.0091 0.006 0.0073 0.0282 0.018 0.001 0.0168 0.0288 0.0115 0.0201 0.0092 0.0067 0.0032 0.0163 0.0057 0.0105 0.0212 0.003
Mitochondria 0.0076 0.0001 0.0131 0.0569 0.0071 0.0009 0.0067 0.0016 0.0016 0.0025 0.0031 0.0011 0.0064 0.0028 0.0048 0.0105 0.0033 0.0017
None 0.0027 0 0.0002 0.0006 0.0002 0.0008 0.001 0.0001 0.0016 0.0001 0.0004 0.0001 0.0024 0.0002 0.0008 0.0009 0.0008 0.0002
Nuclear Periphery 0.0651 0.0057 0.0077 0.0035 0.0079 0.0097 0.0452 0.0069 0.0072 0.0063 0.0053 0.0077 0.0427 0.0093 0.0074 0.0252 0.0054 0.0174
Nucleolus 0.0018 0 0.0001 0.0007 0 0 0.0001 0.0001 0.0009 0.0002 0.0001 0 0.0004 0 0.0001 0.0002 0.0005 0.0001
Nucleus 0.0045 0.0003 0.0004 0.0007 0.0004 0.0005 0.0026 0.0007 0.0012 0.001 0.0008 0.0015 0.0241 0.0007 0.0007 0.0017 0.0004 0.002
Peroxisomes 0.0131 0 0.0067 0.0122 0.031 0.0002 0.0097 0.0002 0.0038 0.0029 0.0015 0.0012 0.0012 0 0.0005 0.002 0.0202 0.0005
Punctate Nuclear 0.0023 0.0001 0.0003 0.0007 0.0005 0.0003 0.0012 0.0002 0.0016 0.0001 0.0012 0.0003 0.0032 0.0001 0.0028 0.0011 0.0061 0.0004
Vacuole 0.0048 0.0024 0.0041 0.0077 0.0044 0.0009 0.0062 0.0055 0.0065 0.0156 0.0064 0.0018 0.0024 0.0024 0.0034 0.0015 0.0022 0.0023
Vacuole Periphery 0.0053 0.0002 0.0013 0.0019 0.0015 0.0004 0.0032 0.0007 0.0006 0.0022 0.0016 0.0004 0.0016 0.0005 0.001 0.0018 0.0008 0.0006

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 27.1966 34.7316 29.2086 45.1442 50.3737 25.8872 39.7993 34.7624 30.1053 50.8133
Translational Efficiency 2.0758 1.6722 1.8922 0.9675 1.1419 2.1836 1.3731 1.6318 1.7763 1.5839

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response, and also shows K1 killer toxin resistance; homologous to worm B0334.15/EMC-5, fly CG15168, human MMGT
Localization
Cell Percentages ER (98%)
Cell Cycle Regulation No
Subcompartmental Group ER-5

Emc5

Emc5


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Emc5-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available