Standard name | |
---|---|
Human Ortholog | |
Description | Membrane-embedded ubiquitin-protein ligase; ER and inner nuclear membrane localized RING-CH domain E3 ligase involved in ER-associated protein degradation (ERAD); targets misfolded cytosolic/nucleoplasmic domains of soluble and membrane embedded proteins (ERAD-C) and a transmembrane domain containing substrate (ERAD-M), Sbh2p; C-terminal element (CTE), conserved in human ortholog MARCH10/TEB4, determines substrate selectivity |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 9 | 27 | 17 | 9 | 1 | 1 | 0 | 0 | – | 0 | 0 | 0 | 7 | 8 | 5 | 5 | 13 | 9 | 9 | 19 | 20 | 14 | 15 | 17 |
Bud | 2 | 10 | 6 | 8 | 3 | 13 | 18 | 19 | – | 16 | 24 | 27 | 0 | 1 | 1 | 2 | 4 | 6 | 2 | 5 | 2 | 6 | 3 | 3 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 2 | 0 | 0 | 1 | 0 | 2 | 5 | 0 | 2 | 5 | 3 | 3 | 1 | 2 |
Bud Site | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | – | 8 | 6 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 14 | 9 | 33 | 16 | 22 | 9 | 5 | 8 | – | 2 | 10 | 6 | 18 | 20 | 21 | 16 | 13 | 13 | 3 | 6 | 11 | 13 | 8 | 10 |
Cytoplasm | 107 | 160 | 126 | 94 | 172 | 260 | 342 | 388 | – | 366 | 257 | 320 | 92 | 112 | 146 | 227 | 236 | 121 | 22 | 54 | 18 | 20 | 16 | 18 |
Endoplasmic Reticulum | 225 | 246 | 283 | 149 | 58 | 65 | 8 | 6 | – | 5 | 5 | 5 | 254 | 181 | 148 | 11 | 15 | 12 | 480 | 354 | 545 | 355 | 495 | 584 |
Endosome | 16 | 3 | 21 | 7 | 30 | 3 | 11 | 5 | – | 2 | 1 | 2 | 4 | 3 | 1 | 9 | 10 | 5 | 3 | 7 | 2 | 4 | 2 | 7 |
Golgi | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 3 | 2 | 5 | 4 | 3 |
Mitochondria | 8 | 32 | 93 | 157 | 131 | 413 | 460 | 564 | – | 421 | 398 | 486 | 1 | 1 | 0 | 49 | 41 | 16 | 18 | 33 | 26 | 81 | 44 | 31 |
Nucleus | 3 | 0 | 2 | 1 | 6 | 2 | 5 | 11 | – | 9 | 8 | 6 | 0 | 0 | 4 | 6 | 17 | 4 | 0 | 1 | 0 | 0 | 1 | 0 |
Nuclear Periphery | 3 | 2 | 6 | 11 | 7 | 5 | 3 | 4 | – | 5 | 7 | 5 | 0 | 1 | 0 | 1 | 5 | 1 | 3 | 3 | 4 | 4 | 6 | 7 |
Nucleolus | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 2 | – | 0 | 1 | 3 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 1 | 1 | 1 | 0 | 4 | 4 | – | 3 | 3 | 4 | 0 | 0 | 0 | 4 | 2 | 0 | 0 | 1 | 0 | 1 | 0 | 0 |
Vac/Vac Membrane | 128 | 28 | 89 | 47 | 159 | 75 | 84 | 74 | – | 36 | 27 | 31 | 19 | 28 | 23 | 48 | 57 | 40 | 12 | 27 | 8 | 34 | 34 | 50 |
Unique Cell Count | 449 | 450 | 535 | 401 | 490 | 685 | 755 | 866 | 679 | 577 | 717 | 354 | 325 | 310 | 327 | 361 | 203 | 565 | 526 | 654 | 552 | 642 | 744 | |
Labelled Cell Count | 515 | 518 | 679 | 501 | 591 | 846 | 941 | 1087 | 873 | 749 | 900 | 395 | 356 | 349 | 380 | 418 | 228 | 565 | 526 | 654 | 552 | 642 | 744 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.6 | 6.2 | 6.3 | 5.8 | 5.4 | 4.0 | 3.6 | 3.6 | – | 3.1 | 3.1 | 3.2 | 8.1 | 7.8 | 7.9 | 5.6 | 6.1 | 7.2 | 7.5 | 8.3 | 8.6 |
Std Deviation (1e-4) | 1.0 | 1.1 | 1.2 | 1.2 | 1.4 | 1.1 | 1.1 | 0.8 | – | 1.0 | 1.0 | 0.9 | 0.9 | 1.0 | 1.1 | 1.0 | 1.2 | 1.5 | 1.6 | 1.6 | 1.5 |
Intensity Change (Log2) | – | – | – | -0.11 | -0.23 | -0.64 | -0.82 | -0.79 | – | -1.01 | -1.01 | -0.97 | 0.36 | 0.31 | 0.33 | -0.17 | -0.03 | 0.19 | 0.26 | 0.4 | 0.45 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -0.9 | -3.6 | 0 | 0 | 0 | – | 0 | 0 | 0 | -1.1 | -0.6 | -1.4 | -1.5 | 0.3 | 0.8 |
Bud | 1.1 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -1.5 | -1.2 | 0 | 0 | 0 | – | 0 | 0 | 0 | -0.7 | 0 | 0.3 | -0.8 | -1.7 | 0.1 |
Cytoplasm | 0 | 4.1 | 5.4 | 8.0 | 8.0 | – | 10.7 | 7.4 | 7.7 | 0.8 | 3.5 | 7.1 | 13.3 | 12.5 | 9.3 |
Endoplasmic Reticulum | -4.8 | -13.9 | 0 | 0 | 0 | – | 0 | 0 | 0 | 5.6 | 0.8 | -1.4 | -14.9 | -15.2 | -11.6 |
Endosome | -1.9 | 1.6 | 0 | 0 | 0 | – | 0 | 0 | 0 | -2.5 | -2.6 | -3.2 | -0.9 | -0.9 | -1.0 |
Golgi | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 7.4 | 3.6 | 0 | 0 | 0 | – | 0 | 0 | 0 | -8.1 | -7.8 | -7.8 | -0.9 | -2.5 | -3.2 |
Nucleus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4.4 | 0 |
Nuclear Periphery | 1.8 | 0.4 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -2.1 | 5.9 | -2.9 | -2.9 | -4.6 | – | -6.5 | -6.5 | -7.3 | -5.0 | -3.3 | -3.8 | -0.8 | -0.3 | 1.0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 5.4904 | 5.557 | 6.0641 | 5.5079 | 5.3247 | 5.8548 | 7.5279 | 8.3054 | 7.7381 | 7.7296 | 7.2265 | 8.1775 | 8.3519 | 8.8097 | 7.9841 | 7.7333 | 8.1451 | 8.1821 |
Actin | 0.0114 | 0.0089 | 0.003 | 0.0005 | 0.0038 | 0.0016 | 0.0439 | 0.0011 | 0.0225 | 0.0001 | 0.004 | 0.0005 | 0.0329 | 0.0009 | 0.0083 | 0.0103 | 0.0013 | 0.002 |
Bud | 0.0001 | 0.0006 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0004 | 0.0001 | 0.0001 | 0.0002 | 0.0015 | 0 |
Bud Neck | 0.0005 | 0.0008 | 0.0007 | 0.001 | 0.0002 | 0.0016 | 0.0006 | 0.0002 | 0.0004 | 0.0003 | 0.0013 | 0.0004 | 0.0012 | 0.0016 | 0.0002 | 0.0071 | 0.0011 | 0.0006 |
Bud Periphery | 0.0002 | 0.0008 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0005 | 0 | 0.0002 | 0 | 0.0003 | 0.0002 | 0.0006 | 0.0003 | 0.0001 | 0.0005 | 0.0023 | 0 |
Bud Site | 0.0004 | 0.0104 | 0.0009 | 0.0004 | 0.0001 | 0.0002 | 0.0009 | 0.0015 | 0.0009 | 0 | 0.0022 | 0 | 0.0028 | 0.002 | 0.0006 | 0.0012 | 0.0077 | 0.0002 |
Cell Periphery | 0.0005 | 0.0018 | 0.001 | 0.0016 | 0.0004 | 0.0003 | 0.0006 | 0.001 | 0.0003 | 0.0002 | 0.0011 | 0.0003 | 0.0009 | 0.0035 | 0.0003 | 0.0011 | 0.0009 | 0.0002 |
Cytoplasm | 0.1389 | 0.2891 | 0.1072 | 0.0517 | 0.016 | 0.0932 | 0.0118 | 0.0539 | 0.0201 | 0.0064 | 0.0142 | 0.0375 | 0.0328 | 0.0829 | 0.0195 | 0.0249 | 0.0586 | 0.0175 |
Cytoplasmic Foci | 0.0098 | 0.012 | 0.0123 | 0.0007 | 0.0395 | 0.0031 | 0.0219 | 0.0027 | 0.0095 | 0.0001 | 0.0076 | 0.0009 | 0.0167 | 0.0045 | 0.0073 | 0.0051 | 0.0044 | 0.0004 |
Eisosomes | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0.0006 | 0 | 0.0001 | 0 | 0.0011 | 0 | 0.0003 | 0.0001 | 0.0001 | 0.0006 | 0 | 0.0001 |
Endoplasmic Reticulum | 0.658 | 0.488 | 0.7749 | 0.8632 | 0.8509 | 0.7948 | 0.7532 | 0.9148 | 0.8918 | 0.9725 | 0.8676 | 0.9264 | 0.7236 | 0.8174 | 0.87 | 0.8967 | 0.8701 | 0.9469 |
Endosome | 0.0325 | 0.0436 | 0.0239 | 0.0049 | 0.0108 | 0.0172 | 0.0458 | 0.0061 | 0.0158 | 0.0014 | 0.0152 | 0.0039 | 0.0396 | 0.0175 | 0.0216 | 0.013 | 0.0183 | 0.0038 |
Golgi | 0.0037 | 0.0046 | 0.0037 | 0.0004 | 0.0018 | 0.0015 | 0.01 | 0.001 | 0.0051 | 0.0001 | 0.0021 | 0.0003 | 0.0081 | 0.004 | 0.0126 | 0.0111 | 0.0047 | 0.0004 |
Lipid Particles | 0.0075 | 0.0052 | 0.0068 | 0.0021 | 0.004 | 0.0029 | 0.0213 | 0.0026 | 0.0075 | 0.0003 | 0.0209 | 0.0008 | 0.0164 | 0.0113 | 0.0119 | 0.0091 | 0.0021 | 0.0005 |
Mitochondria | 0.0039 | 0.0011 | 0.0102 | 0.0004 | 0.0006 | 0.0006 | 0.0034 | 0.0001 | 0.0029 | 0.0001 | 0.0011 | 0.0001 | 0.0023 | 0.0183 | 0.0244 | 0.0022 | 0.0015 | 0.0005 |
None | 0.0108 | 0.0049 | 0.0078 | 0.0002 | 0.0008 | 0.0025 | 0.0047 | 0.0004 | 0.0018 | 0 | 0.0231 | 0.0091 | 0.0098 | 0.0062 | 0.0004 | 0.0022 | 0.0004 | 0.0073 |
Nuclear Periphery | 0.0772 | 0.0658 | 0.0291 | 0.0267 | 0.0377 | 0.0574 | 0.0547 | 0.0096 | 0.0117 | 0.0152 | 0.0135 | 0.0146 | 0.0614 | 0.0182 | 0.0108 | 0.0054 | 0.0065 | 0.0149 |
Nucleolus | 0.0004 | 0.0005 | 0.0001 | 0.0004 | 0.0001 | 0.0001 | 0.0003 | 0 | 0.0002 | 0 | 0.0009 | 0 | 0.0004 | 0.0001 | 0.0001 | 0.0004 | 0.0009 | 0 |
Nucleus | 0.0252 | 0.034 | 0.0079 | 0.0336 | 0.0061 | 0.0138 | 0.0028 | 0.001 | 0.001 | 0.0009 | 0.0029 | 0.0019 | 0.0149 | 0.0016 | 0.0008 | 0.0011 | 0.0029 | 0.002 |
Peroxisomes | 0.0022 | 0.0005 | 0.001 | 0 | 0.0179 | 0.0001 | 0.0066 | 0.0001 | 0.0036 | 0 | 0.0096 | 0 | 0.0201 | 0.0003 | 0.007 | 0.0026 | 0.0003 | 0 |
Punctate Nuclear | 0.0039 | 0.0055 | 0.0015 | 0.0008 | 0.0034 | 0.0019 | 0.0077 | 0.0002 | 0.0016 | 0 | 0.0056 | 0.0004 | 0.0037 | 0.0004 | 0.0002 | 0.0008 | 0.0007 | 0.0001 |
Vacuole | 0.0103 | 0.0187 | 0.0063 | 0.0106 | 0.0047 | 0.0057 | 0.006 | 0.0033 | 0.0024 | 0.002 | 0.0046 | 0.0021 | 0.0084 | 0.008 | 0.0029 | 0.004 | 0.0128 | 0.0021 |
Vacuole Periphery | 0.0025 | 0.0029 | 0.0015 | 0.0006 | 0.0012 | 0.0014 | 0.0026 | 0.0002 | 0.0006 | 0.0004 | 0.0007 | 0.0003 | 0.0029 | 0.0008 | 0.0009 | 0.0005 | 0.0011 | 0.0004 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 63.6687 | 45.5825 | 46.5776 | 58.0518 | 42.4487 | 45.1143 | 59.0587 | 63.4031 | 52.8381 | 58.3514 |
Translational Efficiency | 0.7193 | 0.7852 | 0.8796 | 0.5109 | 0.6467 | 1.0085 | 0.6889 | 0.5681 | 0.6965 | 0.6287 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
247 | 1267 | 888 | 83 | 228 | 1934 | 708 | 1875 | 475 | 3201 | 1596 | 1958 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1003.09 | 842.67 | 1214.54 | 1593.06 | 696.40 | 893.22 | 1130.04 | 1325.07 | 855.88 | 873.21 | 1177.06 | 1336.43 |
Standard Deviation | 127.31 | 149.71 | 155.54 | 246.63 | 485.00 | 120.37 | 196.60 | 198.00 | 380.54 | 135.05 | 179.92 | 207.45 |
Intensity Change Log 2 | — | -0.251411 | 0.275959 | 0.667350 | — | 0.359099 | 0.698386 | 0.928080 | — | 0.030574 | 0.464228 | 0.779942 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.001847 | 0.000434 | 0.000633 | 0.000995 | 0.000694 | 0.000680 | 0.000585 | 0.003721 | 0.001293 | 0.000582 | 0.000611 | 0.003605 |
Bud Neck | 0.003132 | 0.001460 | 0.000882 | 0.002366 | 0.008382 | 0.002194 | 0.002793 | 0.005173 | 0.005652 | 0.001904 | 0.001729 | 0.005054 |
Bud Site | 0.027325 | 0.003404 | 0.005662 | 0.006690 | 0.039540 | 0.002469 | 0.007086 | 0.021145 | 0.033189 | 0.002839 | 0.006294 | 0.020532 |
Cell Periphery | 0.000838 | 0.000759 | 0.001253 | 0.018210 | 0.002393 | 0.000811 | 0.001095 | 0.001569 | 0.001584 | 0.000790 | 0.001183 | 0.002274 |
Cytoplasm | 0.102299* | 0.178371* | 0.025371 | 0.003242 | 0.394828* | 0.127016* | 0.141647* | 0.061931 | 0.242713* | 0.147343* | 0.076952 | 0.059443 |
Cytoplasmic Foci | 0.052873 | 0.053788 | 0.001744 | 0.002522 | 0.127044 | 0.028160 | 0.030696 | 0.011924 | 0.088475 | 0.038304 | 0.014588 | 0.011525 |
Eisosomes | 0.000404 | 0.001054 | 0.000106 | 0.000278 | 0.001333 | 0.000414 | 0.000184 | 0.000145 | 0.000850 | 0.000667 | 0.000140 | 0.000151 |
Endoplasmic Reticulum | 0.452650* | 0.580642* | 0.917558* | 0.866228* | 0.076500 | 0.701149* | 0.598591* | 0.749979* | 0.272098* | 0.653451* | 0.776061* | 0.754907* |
Endosome | 0.035379 | 0.008574 | 0.004530 | 0.017000 | 0.006897 | 0.009208 | 0.009508 | 0.017799 | 0.021707 | 0.008957 | 0.006738 | 0.017765 |
Golgi | 0.018487 | 0.010130 | 0.000820 | 0.004990 | 0.007660 | 0.016003 | 0.002946 | 0.005219 | 0.013290 | 0.013679 | 0.001763 | 0.005209 |
Lipid Particles | 0.030677 | 0.055800 | 0.003565 | 0.030623 | 0.121859* | 0.053143 | 0.052892 | 0.021737 | 0.074444 | 0.054195 | 0.025447 | 0.022113 |
Mitochondria | 0.003262 | 0.006332 | 0.000152 | 0.000024 | 0.007412 | 0.009529 | 0.001077 | 0.001668 | 0.005254 | 0.008263 | 0.000562 | 0.001598 |
Mitotic Spindle | 0.003835 | 0.000570 | 0.000178 | 0.005667 | 0.001631 | 0.000680 | 0.000299 | 0.006177 | 0.002778 | 0.000637 | 0.000232 | 0.006156 |
None | 0.000813 | 0.014946 | 0.000258 | 0.000007 | 0.013009 | 0.004675 | 0.002647 | 0.002631 | 0.006667 | 0.008741 | 0.001318 | 0.002519 |
Nuclear Periphery | 0.015371 | 0.010248 | 0.005705 | 0.001575 | 0.004160 | 0.005864 | 0.023252 | 0.007139 | 0.009990 | 0.007599 | 0.013489 | 0.006903 |
Nuclear Periphery Foci | 0.042771 | 0.019394 | 0.022898 | 0.021558 | 0.004005 | 0.009407 | 0.050787 | 0.047868 | 0.024163 | 0.013360 | 0.035270 | 0.046753 |
Nucleolus | 0.000334 | 0.000683 | 0.000008 | 0.000002 | 0.001500 | 0.000253 | 0.001387 | 0.000179 | 0.000894 | 0.000423 | 0.000620 | 0.000172 |
Nucleus | 0.025280 | 0.031258 | 0.002493 | 0.000154 | 0.149892* | 0.013931 | 0.046864 | 0.009246 | 0.085094 | 0.020789 | 0.022177 | 0.008860 |
Peroxisomes | 0.001765 | 0.003625 | 0.000065 | 0.000039 | 0.009396 | 0.003407 | 0.002127 | 0.000442 | 0.005428 | 0.003493 | 0.000980 | 0.000425 |
Vacuole | 0.177023 | 0.016852 | 0.005659 | 0.017329 | 0.020247 | 0.009325 | 0.023018 | 0.022889 | 0.101770 | 0.012304 | 0.013360 | 0.022653 |
Vacuole Periphery | 0.003636 | 0.001677 | 0.000460 | 0.000501 | 0.001618 | 0.001683 | 0.000518 | 0.001420 | 0.002667 | 0.001680 | 0.000486 | 0.001381 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 3.43 | 2.84 | 1.58 | -1.44 | -0.89 | 0.27 | 0.67 | -4.95 | -5.02 | -5.19 | 3.01 | 2.85 | -3.76 | -5.30 | -5.23 |
Bud Neck | 3.26 | 4.06 | 0.88 | -1.46 | -2.11 | 3.16 | 2.73 | 1.33 | -4.10 | -2.80 | 3.84 | 3.86 | 0.20 | -4.72 | -4.68 |
Bud Site | 3.47 | 3.03 | 2.88 | -1.45 | -0.27 | 3.94 | 3.40 | 1.86 | -9.13 | -5.27 | 5.28 | 4.56 | 2.09 | -9.01 | -6.17 |
Cell Periphery | 0.45 | -1.07 | -1.71 | -1.72 | -1.67 | 5.26 | 3.78 | 2.52 | -3.60 | -1.80 | 4.49 | 1.50 | -1.41 | -3.19 | -2.14 |
Cytoplasm | -6.09 | 6.98 | 9.20 | 29.52 | 8.08 | 15.83 | 13.52 | 19.69 | 11.91 | 8.41 | 7.80 | 13.18 | 14.83 | 18.26 | 2.86 |
Cytoplasmic Foci | 0.05 | 9.36 | 9.02 | 15.42 | -0.62 | 10.99 | 10.27 | 12.88 | 8.00 | 6.19 | 9.02 | 13.32 | 14.03 | 13.83 | 1.79 |
Eisosomes | -1.48 | 1.92 | 0.57 | 1.73 | -1.07 | 3.42 | 4.43 | 4.59 | 3.58 | 1.09 | 0.74 | 4.73 | 4.70 | 2.95 | -0.28 |
Endoplasmic Reticulum | -5.63 | -18.48 | -11.40 | -9.35 | 1.90 | -44.95* | -26.17 | -46.77* | -3.42 | -8.21 | -22.31 | -27.43 | -26.50 | -9.17 | 2.52 |
Endosome | 5.54 | 6.41 | 2.73 | -2.18 | -3.17 | -1.78 | -1.98 | -7.65 | -7.45 | -6.29 | 4.71 | 5.53 | 1.14 | -8.74 | -10.79 |
Golgi | 1.26 | 3.01 | 2.11 | 2.59 | -2.39 | -4.50 | 2.88 | 1.41 | 7.70 | -2.38 | -0.41 | 3.69 | 2.44 | 7.99 | -4.50 |
Lipid Particles | -5.78 | 7.69 | -0.08 | 2.13 | -2.48 | 5.78 | 5.41 | 8.54 | 10.46 | 5.93 | 3.01 | 7.38 | 8.03 | 12.62 | 0.94 |
Mitochondria | -2.24 | 2.20 | 2.31 | 8.30 | 4.26 | -1.17 | 3.95 | 3.53 | 8.04 | -2.25 | -2.63 | 4.44 | 3.31 | 9.67 | -4.33 |
Mitotic Spindle | 2.42 | 2.74 | -0.49 | -0.99 | -1.04 | 1.32 | 1.68 | -2.82 | -4.83 | -5.17 | 2.71 | 3.19 | -2.59 | -4.97 | -5.30 |
None | -5.06 | 1.09 | 1.68 | 5.40 | 3.12 | 2.42 | 2.94 | 3.01 | 1.89 | 0.05 | -0.98 | 3.09 | 2.30 | 4.44 | -1.34 |
Nuclear Periphery | 0.95 | 1.99 | 2.97 | 6.92 | 3.66 | -1.90 | -6.60 | -2.83 | -1.30 | 5.48 | 0.81 | -1.51 | 1.06 | 0.69 | 4.15 |
Nuclear Periphery Foci | 4.25 | 3.50 | 2.20 | -0.39 | 0.08 | -5.09 | -9.97 | -15.28 | -13.41 | 0.41 | 3.58 | -3.19 | -6.11 | -12.09 | -3.41 |
Nucleolus | -2.41 | 3.01 | 3.07 | 5.77 | 2.89 | 5.06 | 0.21 | 5.30 | 0.91 | 1.78 | 3.30 | 0.86 | 5.07 | 3.32 | 1.46 |
Nucleus | -0.86 | 5.39 | 5.97 | 16.55 | 5.09 | 11.08 | 7.93 | 11.38 | 2.65 | 8.94 | 9.36 | 8.83 | 10.92 | 8.02 | 6.02 |
Peroxisomes | -2.20 | 4.73 | 4.81 | 4.52 | 1.27 | 3.91 | 4.56 | 6.05 | 8.83 | 3.01 | 2.19 | 5.57 | 6.61 | 8.12 | 2.14 |
Vacuole | 10.41 | 11.04 | 9.72 | -0.21 | -1.87 | 4.48 | -1.23 | -1.84 | -8.00 | -0.45 | 10.03 | 9.83 | 8.68 | -6.66 | -5.27 |
Vacuole Periphery | 1.35 | 2.28 | 2.27 | 4.46 | -0.13 | -0.12 | 1.82 | 0.29 | 0.94 | -3.60 | 1.18 | 2.81 | 1.54 | 1.21 | -3.32 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|