Standard name
Human Ortholog
Description Beta subunit of the capping protein heterodimer (Cap1p and Cap2p); capping protein (CP) binds to the barbed ends of actin filaments preventing further polymerization; localized predominantly to cortical actin patches; protein increases in abundance and relocalizes from bud neck to plasma membrane upon DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0.56 0.59 0.66 0.5 0.54 0.42 0.42 0.35 0.4 0.4 0.41 0.51 0.64 0.5 0.52 0.5 0.48 0.75 0.72 0.7 0.73 0.67 0.77
Bud – 0.2 0.16 0.22 0.27 0.19 0.12 0.13 0.15 0.15 0.15 0.13 0.16 0.15 0.07 0.14 0.08 0.08 0 0.07 0 0 0 0
Bud Neck – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery – 0.08 0.06 0 0 0.05 0 0 0 0 0 0 0 0.1 0.1 0.05 0.06 0 0 0 0 0 0 0
Cytoplasm – 0 0.06 0.05 0.07 0 0 0 0 0 0 0 0 0 0 0.1 0.08 0.06 0.06 0 0 0 0 0
Endoplasmic Reticulum – 0.08 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome – 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0
Golgi – 0.29 0.42 0.37 0.16 0.36 0.38 0.5 0.15 0.36 0.3 0.26 0.52 0.68 0.79 0.55 0.7 0.71 0 0.07 0.08 0.08 0.12 0.09
Mitochondria – 0.46 0.19 0.43 0.45 0.38 0.43 0.33 0.82 0.55 0.7 0.66 0.23 0.07 0.07 0.1 0.12 0.09 0 0 0 0 0 0
Nucleus – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes – 0.09 0.22 0.2 0.14 0.21 0.16 0.05 0.09 0.1 0 0.08 0.31 0.17 0.12 0.05 0 0 0.05 0 0.12 0.07 0.08 0.07
SpindlePole – 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0
Vac/Vac Membrane – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 303 190 161 152 289 261 225 212 265 195 236 47 173 192 133 109 75 38 477 266 143 189 223
Bud – 106 51 53 83 100 73 69 93 98 75 76 15 42 28 36 17 13 1 45 15 7 10 4
Bud Neck – 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 5 0 0 1 0
Bud Site – 7 9 4 13 16 16 3 19 15 5 9 0 0 0 4 0 0 – – – – – –
Cell Periphery – 42 18 10 7 29 16 15 17 28 17 11 2 27 37 13 14 6 0 0 0 0 0 0
Cytoplasm – 20 20 11 21 10 17 21 8 15 11 5 4 12 17 26 17 10 2 3 2 1 2 1
Endoplasmic Reticulum – 42 18 8 10 15 19 21 9 16 16 25 4 8 14 10 5 6 0 5 2 0 2 0
Endosome – 16 6 3 5 13 17 9 8 21 10 10 6 3 13 5 4 6 2 21 5 4 10 5
Golgi – 154 134 89 48 191 239 268 90 236 146 152 48 184 307 141 152 112 1 49 29 15 34 26
Mitochondria – 249 60 105 137 204 269 176 504 365 344 380 21 20 28 25 25 14 1 21 4 3 5 5
Nucleus – 4 0 0 2 2 1 0 3 1 1 0 0 0 1 0 0 0 0 0 0 0 0 0
Nuclear Periphery – 3 6 0 4 2 7 7 1 5 7 2 0 0 0 0 1 0 0 3 0 0 1 1
Nucleolus – 8 2 2 6 10 20 24 22 20 15 20 0 0 1 5 5 2 0 0 0 0 0 0
Peroxisomes – 48 71 49 42 110 98 27 58 64 16 44 29 46 45 13 2 4 2 9 46 13 21 19
SpindlePole – 4 8 8 15 17 20 14 7 16 6 10 4 11 10 6 9 9 0 3 4 1 1 0
Vac/Vac Membrane – 14 6 1 7 9 4 21 3 8 4 4 1 0 1 3 2 5 0 7 0 1 0 0
Unique Cell Count 540 320 243 304 533 626 536 612 663 489 579 93 272 387 257 216 157 52 660 381 197 285 292
Labelled Cell Count 1020 599 504 552 1017 1077 902 1054 1173 868 984 181 526 694 420 362 262 52 660 381 197 285 292


Actin

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) – 14.3 14.9 14.4 12.0 14.7 14.5 14.9 13.7 14.8 14.9 15.2 15.4 17.8 18.0 18.9 22.8 23.3 16.2 17.7 18.7
Std Deviation (1e-4) – 4.9 4.2 4.4 4.2 4.4 4.4 4.1 4.3 4.4 4.7 5.0 4.7 4.9 5.1 7.1 7.7 8.9 4.4 4.5 4.2
Intensity Change (Log2) – – – -0.05 -0.31 -0.01 -0.04 0.0 -0.12 -0.0 -0.0 0.03 0.05 0.25 0.28 0.35 0.62 0.65 0.12 0.25 0.33

WT3RAP60RAP140RAP220RAP300RAP380RAP460RAP540RAP620RAP7000102030WT3HU80HU120HU1600102030WT3rpd3Δ_1rpd3Δ_2rpd3Δ_30102030AF100AF140AF1800102030
rapamycinhydroxy-urearpd3 knockoutalpha-factorMultiple Condition Time-Course Intensity PlotsGFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 1.7 -2.4 -1.5 -5.2 -4.9 -7.2 -5.7 -5.4 -5.4 -1.5 1.1 -2.6 -1.8 -2.0 -2.4
Bud 1.8 3.5 1.0 -1.8 -1.2 -0.3 -0.5 -0.2 -1.2 0 -0.2 -3.7 -0.6 -2.8 -2.3
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site -0.9 1.0 0.2 -0.2 0 0.2 -0.5 -1.9 -1.3 0 0 0 -1.0 0 0
Cell Periphery -0.8 -2.1 -0.1 -2.4 -2.1 -2.2 -1.0 -1.5 -3.0 0 2.0 1.9 -0.3 0.4 -0.8
Cytoplasm -0.9 0.3 -3.4 -2.7 -1.5 -4.2 -3.2 -2.9 -4.7 -0.7 -1.0 -1.1 1.7 0.7 0.1
Endoplasmic Reticulum -1.3 -1.4 -2.1 -1.9 -1.2 -3.6 -2.6 -1.6 -0.9 0 -1.6 -1.3 -1.0 -1.9 -0.8
Endosome 0 -0.2 0.5 0.8 -0.2 0 1.2 0.2 -0.2 0 0 1.2 0 0 0
Golgi -1.3 -7.2 -1.8 -1.1 2.3 -9.2 -1.9 -3.5 -4.8 1.7 6.3 10.2 3.1 6.5 6.1
Mitochondria 6.3 7.1 6.0 7.4 4.5 18.9 10.8 14.4 13.5 0.8 -4.0 -4.6 -3.0 -2.2 -2.8
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 -0.5 0 0 0 0 0 0 0
Nucleolus 0 0 0 2.5 3.2 2.7 2.4 2.4 2.6 0 0 0 0 0 0
Peroxisomes -0.6 -2.7 -0.5 -2.5 -7.6 -5.3 -5.3 -8.5 -6.3 1.8 -1.6 -3.8 -5.8 -7.0 -5.5
SpindlePole 0.6 1.6 0.6 0.6 0.1 -1.6 -0.1 -1.4 -0.8 0 1.1 0.1 -0.1 1.1 1.8
Vacuole 0 0.4 -0.2 0 1.7 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 11.021 11.262 10.6079 9.7151 8.7184 11.1294 13.5299 14.0686 13.5969 14.5703 15.3868 14.288 12.7883 11.3473 11.423 11.7213 11.3535 12.0018
Actin 0.8472 0.8801 0.863 0.9448 0.9328 0.9615 0.8477 0.848 0.8932 0.9293 0.8523 0.914 0.8169 0.847 0.9499 0.9681 0.8426 0.9708
Bud 0.0011 0.0023 0.0179 0.0023 0.0004 0.0005 0.0006 0.0037 0.0128 0.0239 0.0026 0.0011 0.0008 0.0036 0.0022 0.0089 0.0041 0.0002
Bud Neck 0.0009 0.0021 0.0031 0.0009 0.0002 0.0034 0.0012 0.0007 0.003 0.0004 0.0231 0.0025 0.0024 0.0052 0.0006 0.0001 0.0009 0.0011
Bud Periphery 0.0016 0.0015 0.0152 0.0059 0.0004 0.0008 0.0007 0.0018 0.0105 0.0219 0.0045 0.0009 0.0009 0.0023 0.0029 0.0107 0.0054 0.0002
Bud Site 0.0034 0.0247 0.0224 0.0019 0.001 0.0006 0.0017 0.0402 0.0122 0.0024 0.0122 0.0005 0.0046 0.0353 0.0089 0.0031 0.0018 0.0003
Cell Periphery 0.0001 0.0001 0.0003 0.0001 0 0.0001 0.0003 0.0001 0.0003 0.0001 0.001 0.0002 0.0009 0.0001 0.0001 0.0001 0.0001 0
Cytoplasm 0.0044 0.0019 0.0013 0.0002 0 0.0001 0.003 0.0157 0.0009 0.0003 0.0009 0.0097 0.0206 0.0018 0.0001 0.0003 0.0007 0
Cytoplasmic Foci 0.0216 0.0408 0.0112 0.0053 0.0037 0.0022 0.01 0.0208 0.0154 0.0018 0.0431 0.0104 0.0086 0.0314 0.0063 0.0018 0.0326 0.0022
Eisosomes 0.0009 0.0003 0.0003 0.0005 0.0003 0.0005 0.0055 0.0002 0.0005 0.0002 0.0003 0.0004 0.001 0.0003 0.0002 0.0002 0.0004 0.0004
Endoplasmic Reticulum 0.0017 0.0001 0.0003 0.0001 0 0 0.0064 0.0003 0.0018 0 0.0011 0.0011 0.0214 0.0001 0 0.0001 0 0
Endosome 0.0086 0.0051 0.0042 0.0027 0.0001 0.0022 0.0124 0.0007 0.0036 0.0002 0.0101 0.0029 0.0141 0.0011 0.0001 0.0003 0.0014 0.0004
Golgi 0.0107 0.0127 0.0094 0.0072 0.0015 0.023 0.0106 0.0045 0.0053 0.0027 0.0138 0.0086 0.0129 0.0139 0.0016 0.0013 0.01 0.0066
Lipid Particles 0.0177 0.0036 0.0053 0.0016 0.0013 0.0003 0.0036 0.0007 0.0026 0.0002 0.0095 0.002 0.0045 0.0008 0.0004 0.0003 0.0078 0.0004
Mitochondria 0.0218 0.0007 0.0064 0.016 0.0274 0.0016 0.0042 0.0008 0.0141 0.0003 0.004 0.0068 0.0058 0.0009 0.0005 0.0008 0.0055 0.0032
None 0.0062 0.0001 0.0009 0.0001 0 0 0.0224 0.0001 0.0023 0 0.0004 0.0123 0.01 0.0002 0 0.0001 0.0021 0
Nuclear Periphery 0.0013 0.0001 0.0011 0 0 0 0.0192 0.0007 0.003 0 0.0051 0.0009 0.0314 0.0001 0 0.0001 0 0
Nucleolus 0.0005 0 0.0003 0 0 0 0.0004 0 0.0002 0 0.0002 0.0002 0.0007 0 0 0 0 0
Nucleus 0.0009 0.0001 0.0009 0 0 0 0.0031 0.0017 0.001 0 0.0003 0.0004 0.0125 0.0002 0 0.0001 0 0
Peroxisomes 0.045 0.0199 0.0317 0.01 0.0308 0.003 0.0428 0.0584 0.0157 0.0162 0.0124 0.0232 0.0135 0.0535 0.0261 0.0034 0.0838 0.0141
Punctate Nuclear 0.0035 0.0037 0.0045 0.0001 0 0 0.0024 0.0006 0.0012 0 0.002 0.0014 0.0123 0.0021 0.0001 0.0001 0.0006 0
Vacuole 0.0006 0.0001 0.0003 0.0002 0 0 0.0007 0.0001 0.0005 0.0001 0.0008 0.0003 0.0018 0 0 0.0002 0 0
Vacuole Periphery 0.0003 0 0.0001 0.0001 0 0 0.0011 0 0.0003 0 0.0005 0.0002 0.0025 0 0 0.0001 0 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 58.1075 46.8072 36.4273 58.4002 42.2229 35.7387 50.2125 46.7449 56.121 63.6868
Translational Efficiency 1.2889 1.0834 1.7309 0.9371 1.6293 2.7832 1.4462 1.5751 1.5901 1.3885

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
168 819 2626 1902 2451 1649 2134 1906 2619 2468 4760 3808

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1997.65 1176.38 1502.36 1765.10 1265.65 1394.22 1470.36 1918.13 1312.61 1321.93 1488.01 1841.70
Standard Deviation 480.48 282.41 316.58 380.62 257.00 272.95 369.28 385.63 329.83 294.56 341.59 390.70
Intensity Change Log 2 — -0.763950 -0.411073 -0.178554 — 0.139580 0.216291 0.599822 — -0.344226 -0.134548 0.174640

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.708184 0.796377 0.773784 0.884032 0.917095 0.889812 0.766543 0.871571 0.903694 0.858806 0.770538 0.877795
Bud Neck 0.006799 0.003039 0.005687 0.003593 0.001398 0.001847 0.006917 0.004296 0.001745 0.002242 0.006238 0.003945
Bud Site 0.020090 0.013994 0.062932 0.050562 0.008016 0.012839 0.112280 0.050659 0.008790 0.013222 0.085056 0.050611
Cell Periphery 0.001005 0.000308 0.000685 0.002224 0.000358 0.000325 0.001119 0.001392 0.000400 0.000319 0.000880 0.001808
Cytoplasm 0.001167 0.002205 0.009725 0.004396 0.000983 0.001003 0.010320 0.003392 0.000994 0.001402 0.009992 0.003894
Cytoplasmic Foci 0.008363 0.015243 0.011661 0.003077 0.003007 0.006877 0.006713 0.004004 0.003351 0.009653 0.009443 0.003541
Eisosomes 0.007835 0.001426 0.000925 0.011119 0.002904 0.001720 0.000990 0.007536 0.003221 0.001623 0.000954 0.009326
Endoplasmic Reticulum 0.013094 0.001024 0.001097 0.002924 0.000446 0.000746 0.001310 0.002641 0.001257 0.000839 0.001192 0.002783
Endosome 0.026493 0.027405 0.017036 0.005062 0.003222 0.008918 0.008116 0.009613 0.004715 0.015053 0.013037 0.007339
Golgi 0.137292 0.045067 0.014143 0.007364 0.013070 0.021119 0.010417 0.009873 0.021038 0.029066 0.012473 0.008620
Lipid Particles 0.045025 0.037617 0.020606 0.002854 0.007479 0.009120 0.005486 0.010234 0.009888 0.018576 0.013828 0.006548
Mitochondria 0.011367 0.013758 0.032195 0.010826 0.020866 0.011527 0.034541 0.010731 0.020256 0.012268 0.033247 0.010779
Mitotic Spindle 0.000369 0.000929 0.005958 0.001901 0.000304 0.000525 0.005484 0.001277 0.000308 0.000659 0.005746 0.001589
None 0.000641 0.000188 0.000082 0.000042 0.000070 0.000068 0.000084 0.000037 0.000107 0.000108 0.000083 0.000039
Nuclear Periphery 0.000086 0.000024 0.000058 0.000020 0.000011 0.000015 0.000049 0.000023 0.000016 0.000018 0.000054 0.000022
Nuclear Periphery Foci 0.000074 0.000142 0.000100 0.000041 0.000011 0.000035 0.000055 0.000035 0.000015 0.000071 0.000080 0.000038
Nucleolus 0.000065 0.000037 0.000016 0.000006 0.000007 0.000010 0.000015 0.000006 0.000011 0.000019 0.000016 0.000006
Nucleus 0.003648 0.000037 0.000061 0.000032 0.000020 0.000072 0.000061 0.000029 0.000253 0.000060 0.000061 0.000030
Peroxisomes 0.005777 0.040355 0.041164 0.008356 0.020347 0.033066 0.027612 0.011424 0.019412 0.035485 0.035089 0.009892
Vacuole 0.001612 0.000547 0.001514 0.000864 0.000228 0.000189 0.001505 0.000895 0.000317 0.000308 0.001510 0.000879
Vacuole Periphery 0.001015 0.000278 0.000569 0.000706 0.000157 0.000166 0.000381 0.000330 0.000212 0.000204 0.000485 0.000518

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.45 -2.67 -7.23 -7.30 -13.86 4.27 20.02 7.18 2.51 -12.28 7.04 22.80 4.99 -2.86 -18.41
Bud Neck 2.82 0.85 2.58 -0.81 3.55 -1.44 -9.92 -6.54 -4.91 3.94 -1.57 -11.43 -6.85 -4.41 5.18
Bud Site 1.05 -7.04 -4.90 -9.21 2.78 -2.59 -22.16 -13.01 -10.84 11.09 -2.77 -26.82 -16.97 -14.40 9.85
Cell Periphery 3.67 1.58 -3.60 -6.64 -5.21 0.28 -4.46 -5.00 -5.99 -1.22 0.73 -3.80 -7.11 -8.76 -5.08
Cytoplasm -0.70 -6.81 -2.73 -2.41 4.49 0.09 -9.44 -4.40 -4.90 6.58 -1.09 -12.32 -5.66 -5.22 7.62
Cytoplasmic Foci -1.16 -0.37 2.02 6.38 8.64 -4.10 -4.74 -1.61 2.70 3.06 -6.55 -8.55 -0.24 6.56 8.72
Eisosomes 4.65 5.04 -1.70 -6.97 -7.36 3.69 6.45 -3.76 -4.82 -5.46 5.27 7.87 -6.36 -8.36 -9.13
Endoplasmic Reticulum 5.02 4.98 4.12 -3.35 -3.12 -2.21 -3.77 -4.62 -3.86 -2.55 2.04 0.24 -3.82 -5.17 -4.06
Endosome -0.18 1.94 4.51 7.44 9.52 -5.74 -6.76 -6.01 -0.55 -1.26 -8.27 -10.40 -3.58 5.82 6.31
Golgi 5.49 7.54 7.96 9.06 4.38 -3.78 1.70 2.09 5.30 0.33 -3.36 4.89 7.06 10.33 3.51
Lipid Particles 0.76 2.70 4.77 7.66 9.28 -1.06 1.51 -1.78 -0.69 -3.06 -4.30 -2.70 2.55 6.40 5.65
Mitochondria -0.46 -4.05 0.10 1.07 9.02 4.42 -4.81 5.02 0.42 8.94 4.08 -5.95 5.48 0.98 12.68
Mitotic Spindle -1.55 -8.32 -3.01 -1.71 5.13 -1.03 -6.66 -4.47 -2.90 5.01 -1.93 -10.77 -4.93 -3.20 7.28
None 1.04 1.41 1.51 1.63 6.15 0.00 -1.52 6.56 1.74 5.48 -0.25 0.87 2.60 2.10 8.31
Nuclear Periphery 4.23 1.71 4.50 0.78 5.97 -0.77 -5.75 -4.20 -1.54 3.67 -0.52 -8.25 -2.35 -1.00 6.84
Nuclear Periphery Foci -1.22 -1.20 1.71 1.92 2.58 -1.46 -5.02 -2.70 0.41 1.49 -2.64 -5.22 -2.82 1.56 2.99
Nucleolus 0.50 1.01 1.23 1.27 3.45 -0.96 -2.71 0.58 1.01 3.21 -1.19 -1.42 1.33 1.44 4.79
Nucleus 1.01 1.00 1.01 0.73 2.94 -0.99 -1.98 -1.30 0.96 1.75 0.02 0.73 0.97 0.97 2.79
Peroxisomes -7.51 -11.22 -0.70 7.84 13.31 -4.08 -2.90 4.20 7.04 6.61 -6.00 -7.43 5.60 10.63 14.29
Vacuole 1.96 -0.01 1.36 -1.45 2.37 0.28 -3.55 -2.96 -3.21 1.64 0.12 -5.22 -3.39 -3.81 2.72
Vacuole Periphery 4.75 2.00 1.02 -1.64 -0.45 -0.20 -3.14 -1.58 -1.50 0.48 0.19 -2.79 -2.16 -2.24 -0.17
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Beta subunit of the capping protein heterodimer (Cap1p and Cap2p); capping protein (CP) binds to the barbed ends of actin filaments preventing further polymerization; localized predominantly to cortical actin patches; protein increases in abundance and relocalizes from bud neck to plasma membrane upon DNA replication stress
Localization
Cell Percentages actin (94%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Cap2

Cap2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cap2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available