Standard name | |
---|---|
Human Ortholog | |
Description | Beta subunit of the capping protein heterodimer (Cap1p and Cap2p); capping protein (CP) binds to the barbed ends of actin filaments preventing further polymerization; localized predominantly to cortical actin patches; protein increases in abundance and relocalizes from bud neck to plasma membrane upon DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | – | 303 | 190 | 161 | 152 | 289 | 261 | 225 | 212 | 265 | 195 | 236 | 47 | 173 | 192 | 133 | 109 | 75 | 38 | 477 | 266 | 143 | 189 | 223 |
Bud | – | 106 | 51 | 53 | 83 | 100 | 73 | 69 | 93 | 98 | 75 | 76 | 15 | 42 | 28 | 36 | 17 | 13 | 1 | 45 | 15 | 7 | 10 | 4 |
Bud Neck | – | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 5 | 0 | 0 | 1 | 0 |
Bud Site | – | 7 | 9 | 4 | 13 | 16 | 16 | 3 | 19 | 15 | 5 | 9 | 0 | 0 | 0 | 4 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | – | 42 | 18 | 10 | 7 | 29 | 16 | 15 | 17 | 28 | 17 | 11 | 2 | 27 | 37 | 13 | 14 | 6 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | – | 20 | 20 | 11 | 21 | 10 | 17 | 21 | 8 | 15 | 11 | 5 | 4 | 12 | 17 | 26 | 17 | 10 | 2 | 3 | 2 | 1 | 2 | 1 |
Endoplasmic Reticulum | – | 42 | 18 | 8 | 10 | 15 | 19 | 21 | 9 | 16 | 16 | 25 | 4 | 8 | 14 | 10 | 5 | 6 | 0 | 5 | 2 | 0 | 2 | 0 |
Endosome | – | 16 | 6 | 3 | 5 | 13 | 17 | 9 | 8 | 21 | 10 | 10 | 6 | 3 | 13 | 5 | 4 | 6 | 2 | 21 | 5 | 4 | 10 | 5 |
Golgi | – | 154 | 134 | 89 | 48 | 191 | 239 | 268 | 90 | 236 | 146 | 152 | 48 | 184 | 307 | 141 | 152 | 112 | 1 | 49 | 29 | 15 | 34 | 26 |
Mitochondria | – | 249 | 60 | 105 | 137 | 204 | 269 | 176 | 504 | 365 | 344 | 380 | 21 | 20 | 28 | 25 | 25 | 14 | 1 | 21 | 4 | 3 | 5 | 5 |
Nucleus | – | 4 | 0 | 0 | 2 | 2 | 1 | 0 | 3 | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | – | 3 | 6 | 0 | 4 | 2 | 7 | 7 | 1 | 5 | 7 | 2 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 3 | 0 | 0 | 1 | 1 |
Nucleolus | – | 8 | 2 | 2 | 6 | 10 | 20 | 24 | 22 | 20 | 15 | 20 | 0 | 0 | 1 | 5 | 5 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | – | 48 | 71 | 49 | 42 | 110 | 98 | 27 | 58 | 64 | 16 | 44 | 29 | 46 | 45 | 13 | 2 | 4 | 2 | 9 | 46 | 13 | 21 | 19 |
SpindlePole | – | 4 | 8 | 8 | 15 | 17 | 20 | 14 | 7 | 16 | 6 | 10 | 4 | 11 | 10 | 6 | 9 | 9 | 0 | 3 | 4 | 1 | 1 | 0 |
Vac/Vac Membrane | – | 14 | 6 | 1 | 7 | 9 | 4 | 21 | 3 | 8 | 4 | 4 | 1 | 0 | 1 | 3 | 2 | 5 | 0 | 7 | 0 | 1 | 0 | 0 |
Unique Cell Count | 540 | 320 | 243 | 304 | 533 | 626 | 536 | 612 | 663 | 489 | 579 | 93 | 272 | 387 | 257 | 216 | 157 | 52 | 660 | 381 | 197 | 285 | 292 | |
Labelled Cell Count | 1020 | 599 | 504 | 552 | 1017 | 1077 | 902 | 1054 | 1173 | 868 | 984 | 181 | 526 | 694 | 420 | 362 | 262 | 52 | 660 | 381 | 197 | 285 | 292 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | – | 14.3 | 14.9 | 14.4 | 12.0 | 14.7 | 14.5 | 14.9 | 13.7 | 14.8 | 14.9 | 15.2 | 15.4 | 17.8 | 18.0 | 18.9 | 22.8 | 23.3 | 16.2 | 17.7 | 18.7 |
Std Deviation (1e-4) | – | 4.9 | 4.2 | 4.4 | 4.2 | 4.4 | 4.4 | 4.1 | 4.3 | 4.4 | 4.7 | 5.0 | 4.7 | 4.9 | 5.1 | 7.1 | 7.7 | 8.9 | 4.4 | 4.5 | 4.2 |
Intensity Change (Log2) | – | – | – | -0.05 | -0.31 | -0.01 | -0.04 | 0.0 | -0.12 | -0.0 | -0.0 | 0.03 | 0.05 | 0.25 | 0.28 | 0.35 | 0.62 | 0.65 | 0.12 | 0.25 | 0.33 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 1.7 | -2.4 | -1.5 | -5.2 | -4.9 | -7.2 | -5.7 | -5.4 | -5.4 | -1.5 | 1.1 | -2.6 | -1.8 | -2.0 | -2.4 |
Bud | 1.8 | 3.5 | 1.0 | -1.8 | -1.2 | -0.3 | -0.5 | -0.2 | -1.2 | 0 | -0.2 | -3.7 | -0.6 | -2.8 | -2.3 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | -0.9 | 1.0 | 0.2 | -0.2 | 0 | 0.2 | -0.5 | -1.9 | -1.3 | 0 | 0 | 0 | -1.0 | 0 | 0 |
Cell Periphery | -0.8 | -2.1 | -0.1 | -2.4 | -2.1 | -2.2 | -1.0 | -1.5 | -3.0 | 0 | 2.0 | 1.9 | -0.3 | 0.4 | -0.8 |
Cytoplasm | -0.9 | 0.3 | -3.4 | -2.7 | -1.5 | -4.2 | -3.2 | -2.9 | -4.7 | -0.7 | -1.0 | -1.1 | 1.7 | 0.7 | 0.1 |
Endoplasmic Reticulum | -1.3 | -1.4 | -2.1 | -1.9 | -1.2 | -3.6 | -2.6 | -1.6 | -0.9 | 0 | -1.6 | -1.3 | -1.0 | -1.9 | -0.8 |
Endosome | 0 | -0.2 | 0.5 | 0.8 | -0.2 | 0 | 1.2 | 0.2 | -0.2 | 0 | 0 | 1.2 | 0 | 0 | 0 |
Golgi | -1.3 | -7.2 | -1.8 | -1.1 | 2.3 | -9.2 | -1.9 | -3.5 | -4.8 | 1.7 | 6.3 | 10.2 | 3.1 | 6.5 | 6.1 |
Mitochondria | 6.3 | 7.1 | 6.0 | 7.4 | 4.5 | 18.9 | 10.8 | 14.4 | 13.5 | 0.8 | -4.0 | -4.6 | -3.0 | -2.2 | -2.8 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -0.5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 2.5 | 3.2 | 2.7 | 2.4 | 2.4 | 2.6 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | -0.6 | -2.7 | -0.5 | -2.5 | -7.6 | -5.3 | -5.3 | -8.5 | -6.3 | 1.8 | -1.6 | -3.8 | -5.8 | -7.0 | -5.5 |
SpindlePole | 0.6 | 1.6 | 0.6 | 0.6 | 0.1 | -1.6 | -0.1 | -1.4 | -0.8 | 0 | 1.1 | 0.1 | -0.1 | 1.1 | 1.8 |
Vacuole | 0 | 0.4 | -0.2 | 0 | 1.7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 11.021 | 11.262 | 10.6079 | 9.7151 | 8.7184 | 11.1294 | 13.5299 | 14.0686 | 13.5969 | 14.5703 | 15.3868 | 14.288 | 12.7883 | 11.3473 | 11.423 | 11.7213 | 11.3535 | 12.0018 |
Actin | 0.8472 | 0.8801 | 0.863 | 0.9448 | 0.9328 | 0.9615 | 0.8477 | 0.848 | 0.8932 | 0.9293 | 0.8523 | 0.914 | 0.8169 | 0.847 | 0.9499 | 0.9681 | 0.8426 | 0.9708 |
Bud | 0.0011 | 0.0023 | 0.0179 | 0.0023 | 0.0004 | 0.0005 | 0.0006 | 0.0037 | 0.0128 | 0.0239 | 0.0026 | 0.0011 | 0.0008 | 0.0036 | 0.0022 | 0.0089 | 0.0041 | 0.0002 |
Bud Neck | 0.0009 | 0.0021 | 0.0031 | 0.0009 | 0.0002 | 0.0034 | 0.0012 | 0.0007 | 0.003 | 0.0004 | 0.0231 | 0.0025 | 0.0024 | 0.0052 | 0.0006 | 0.0001 | 0.0009 | 0.0011 |
Bud Periphery | 0.0016 | 0.0015 | 0.0152 | 0.0059 | 0.0004 | 0.0008 | 0.0007 | 0.0018 | 0.0105 | 0.0219 | 0.0045 | 0.0009 | 0.0009 | 0.0023 | 0.0029 | 0.0107 | 0.0054 | 0.0002 |
Bud Site | 0.0034 | 0.0247 | 0.0224 | 0.0019 | 0.001 | 0.0006 | 0.0017 | 0.0402 | 0.0122 | 0.0024 | 0.0122 | 0.0005 | 0.0046 | 0.0353 | 0.0089 | 0.0031 | 0.0018 | 0.0003 |
Cell Periphery | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0 | 0.0001 | 0.0003 | 0.0001 | 0.0003 | 0.0001 | 0.001 | 0.0002 | 0.0009 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 |
Cytoplasm | 0.0044 | 0.0019 | 0.0013 | 0.0002 | 0 | 0.0001 | 0.003 | 0.0157 | 0.0009 | 0.0003 | 0.0009 | 0.0097 | 0.0206 | 0.0018 | 0.0001 | 0.0003 | 0.0007 | 0 |
Cytoplasmic Foci | 0.0216 | 0.0408 | 0.0112 | 0.0053 | 0.0037 | 0.0022 | 0.01 | 0.0208 | 0.0154 | 0.0018 | 0.0431 | 0.0104 | 0.0086 | 0.0314 | 0.0063 | 0.0018 | 0.0326 | 0.0022 |
Eisosomes | 0.0009 | 0.0003 | 0.0003 | 0.0005 | 0.0003 | 0.0005 | 0.0055 | 0.0002 | 0.0005 | 0.0002 | 0.0003 | 0.0004 | 0.001 | 0.0003 | 0.0002 | 0.0002 | 0.0004 | 0.0004 |
Endoplasmic Reticulum | 0.0017 | 0.0001 | 0.0003 | 0.0001 | 0 | 0 | 0.0064 | 0.0003 | 0.0018 | 0 | 0.0011 | 0.0011 | 0.0214 | 0.0001 | 0 | 0.0001 | 0 | 0 |
Endosome | 0.0086 | 0.0051 | 0.0042 | 0.0027 | 0.0001 | 0.0022 | 0.0124 | 0.0007 | 0.0036 | 0.0002 | 0.0101 | 0.0029 | 0.0141 | 0.0011 | 0.0001 | 0.0003 | 0.0014 | 0.0004 |
Golgi | 0.0107 | 0.0127 | 0.0094 | 0.0072 | 0.0015 | 0.023 | 0.0106 | 0.0045 | 0.0053 | 0.0027 | 0.0138 | 0.0086 | 0.0129 | 0.0139 | 0.0016 | 0.0013 | 0.01 | 0.0066 |
Lipid Particles | 0.0177 | 0.0036 | 0.0053 | 0.0016 | 0.0013 | 0.0003 | 0.0036 | 0.0007 | 0.0026 | 0.0002 | 0.0095 | 0.002 | 0.0045 | 0.0008 | 0.0004 | 0.0003 | 0.0078 | 0.0004 |
Mitochondria | 0.0218 | 0.0007 | 0.0064 | 0.016 | 0.0274 | 0.0016 | 0.0042 | 0.0008 | 0.0141 | 0.0003 | 0.004 | 0.0068 | 0.0058 | 0.0009 | 0.0005 | 0.0008 | 0.0055 | 0.0032 |
None | 0.0062 | 0.0001 | 0.0009 | 0.0001 | 0 | 0 | 0.0224 | 0.0001 | 0.0023 | 0 | 0.0004 | 0.0123 | 0.01 | 0.0002 | 0 | 0.0001 | 0.0021 | 0 |
Nuclear Periphery | 0.0013 | 0.0001 | 0.0011 | 0 | 0 | 0 | 0.0192 | 0.0007 | 0.003 | 0 | 0.0051 | 0.0009 | 0.0314 | 0.0001 | 0 | 0.0001 | 0 | 0 |
Nucleolus | 0.0005 | 0 | 0.0003 | 0 | 0 | 0 | 0.0004 | 0 | 0.0002 | 0 | 0.0002 | 0.0002 | 0.0007 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0.0009 | 0.0001 | 0.0009 | 0 | 0 | 0 | 0.0031 | 0.0017 | 0.001 | 0 | 0.0003 | 0.0004 | 0.0125 | 0.0002 | 0 | 0.0001 | 0 | 0 |
Peroxisomes | 0.045 | 0.0199 | 0.0317 | 0.01 | 0.0308 | 0.003 | 0.0428 | 0.0584 | 0.0157 | 0.0162 | 0.0124 | 0.0232 | 0.0135 | 0.0535 | 0.0261 | 0.0034 | 0.0838 | 0.0141 |
Punctate Nuclear | 0.0035 | 0.0037 | 0.0045 | 0.0001 | 0 | 0 | 0.0024 | 0.0006 | 0.0012 | 0 | 0.002 | 0.0014 | 0.0123 | 0.0021 | 0.0001 | 0.0001 | 0.0006 | 0 |
Vacuole | 0.0006 | 0.0001 | 0.0003 | 0.0002 | 0 | 0 | 0.0007 | 0.0001 | 0.0005 | 0.0001 | 0.0008 | 0.0003 | 0.0018 | 0 | 0 | 0.0002 | 0 | 0 |
Vacuole Periphery | 0.0003 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0.0011 | 0 | 0.0003 | 0 | 0.0005 | 0.0002 | 0.0025 | 0 | 0 | 0.0001 | 0 | 0 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 58.1075 | 46.8072 | 36.4273 | 58.4002 | 42.2229 | 35.7387 | 50.2125 | 46.7449 | 56.121 | 63.6868 |
Translational Efficiency | 1.2889 | 1.0834 | 1.7309 | 0.9371 | 1.6293 | 2.7832 | 1.4462 | 1.5751 | 1.5901 | 1.3885 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
168 | 819 | 2626 | 1902 | 2451 | 1649 | 2134 | 1906 | 2619 | 2468 | 4760 | 3808 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1997.65 | 1176.38 | 1502.36 | 1765.10 | 1265.65 | 1394.22 | 1470.36 | 1918.13 | 1312.61 | 1321.93 | 1488.01 | 1841.70 |
Standard Deviation | 480.48 | 282.41 | 316.58 | 380.62 | 257.00 | 272.95 | 369.28 | 385.63 | 329.83 | 294.56 | 341.59 | 390.70 |
Intensity Change Log 2 | — | -0.763950 | -0.411073 | -0.178554 | — | 0.139580 | 0.216291 | 0.599822 | — | -0.344226 | -0.134548 | 0.174640 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.708184* | 0.796377* | 0.773784* | 0.884032* | 0.917095* | 0.889812* | 0.766543* | 0.871571* | 0.903694* | 0.858806* | 0.770538* | 0.877795* |
Bud Neck | 0.006799 | 0.003039 | 0.005687 | 0.003593 | 0.001398 | 0.001847 | 0.006917 | 0.004296 | 0.001745 | 0.002242 | 0.006238 | 0.003945 |
Bud Site | 0.020090 | 0.013994 | 0.062932 | 0.050562 | 0.008016 | 0.012839 | 0.112280* | 0.050659 | 0.008790 | 0.013222 | 0.085056 | 0.050611 |
Cell Periphery | 0.001005 | 0.000308 | 0.000685 | 0.002224 | 0.000358 | 0.000325 | 0.001119 | 0.001392 | 0.000400 | 0.000319 | 0.000880 | 0.001808 |
Cytoplasm | 0.001167 | 0.002205 | 0.009725 | 0.004396 | 0.000983 | 0.001003 | 0.010320 | 0.003392 | 0.000994 | 0.001402 | 0.009992 | 0.003894 |
Cytoplasmic Foci | 0.008363 | 0.015243 | 0.011661 | 0.003077 | 0.003007 | 0.006877 | 0.006713 | 0.004004 | 0.003351 | 0.009653 | 0.009443 | 0.003541 |
Eisosomes | 0.007835 | 0.001426 | 0.000925 | 0.011119 | 0.002904 | 0.001720 | 0.000990 | 0.007536 | 0.003221 | 0.001623 | 0.000954 | 0.009326 |
Endoplasmic Reticulum | 0.013094 | 0.001024 | 0.001097 | 0.002924 | 0.000446 | 0.000746 | 0.001310 | 0.002641 | 0.001257 | 0.000839 | 0.001192 | 0.002783 |
Endosome | 0.026493 | 0.027405 | 0.017036 | 0.005062 | 0.003222 | 0.008918 | 0.008116 | 0.009613 | 0.004715 | 0.015053 | 0.013037 | 0.007339 |
Golgi | 0.137292* | 0.045067 | 0.014143 | 0.007364 | 0.013070 | 0.021119 | 0.010417 | 0.009873 | 0.021038 | 0.029066 | 0.012473 | 0.008620 |
Lipid Particles | 0.045025 | 0.037617 | 0.020606 | 0.002854 | 0.007479 | 0.009120 | 0.005486 | 0.010234 | 0.009888 | 0.018576 | 0.013828 | 0.006548 |
Mitochondria | 0.011367 | 0.013758 | 0.032195 | 0.010826 | 0.020866 | 0.011527 | 0.034541 | 0.010731 | 0.020256 | 0.012268 | 0.033247 | 0.010779 |
Mitotic Spindle | 0.000369 | 0.000929 | 0.005958 | 0.001901 | 0.000304 | 0.000525 | 0.005484 | 0.001277 | 0.000308 | 0.000659 | 0.005746 | 0.001589 |
None | 0.000641 | 0.000188 | 0.000082 | 0.000042 | 0.000070 | 0.000068 | 0.000084 | 0.000037 | 0.000107 | 0.000108 | 0.000083 | 0.000039 |
Nuclear Periphery | 0.000086 | 0.000024 | 0.000058 | 0.000020 | 0.000011 | 0.000015 | 0.000049 | 0.000023 | 0.000016 | 0.000018 | 0.000054 | 0.000022 |
Nuclear Periphery Foci | 0.000074 | 0.000142 | 0.000100 | 0.000041 | 0.000011 | 0.000035 | 0.000055 | 0.000035 | 0.000015 | 0.000071 | 0.000080 | 0.000038 |
Nucleolus | 0.000065 | 0.000037 | 0.000016 | 0.000006 | 0.000007 | 0.000010 | 0.000015 | 0.000006 | 0.000011 | 0.000019 | 0.000016 | 0.000006 |
Nucleus | 0.003648 | 0.000037 | 0.000061 | 0.000032 | 0.000020 | 0.000072 | 0.000061 | 0.000029 | 0.000253 | 0.000060 | 0.000061 | 0.000030 |
Peroxisomes | 0.005777 | 0.040355 | 0.041164 | 0.008356 | 0.020347 | 0.033066 | 0.027612 | 0.011424 | 0.019412 | 0.035485 | 0.035089 | 0.009892 |
Vacuole | 0.001612 | 0.000547 | 0.001514 | 0.000864 | 0.000228 | 0.000189 | 0.001505 | 0.000895 | 0.000317 | 0.000308 | 0.001510 | 0.000879 |
Vacuole Periphery | 0.001015 | 0.000278 | 0.000569 | 0.000706 | 0.000157 | 0.000166 | 0.000381 | 0.000330 | 0.000212 | 0.000204 | 0.000485 | 0.000518 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -3.45 | -2.67 | -7.23 | -7.30 | -13.86 | 4.27 | 20.02* | 7.18 | 2.51 | -12.28 | 7.04 | 22.80* | 4.99 | -2.86 | -18.41 |
Bud Neck | 2.82 | 0.85 | 2.58 | -0.81 | 3.55 | -1.44 | -9.92 | -6.54 | -4.91 | 3.94 | -1.57 | -11.43 | -6.85 | -4.41 | 5.18 |
Bud Site | 1.05 | -7.04 | -4.90 | -9.21 | 2.78 | -2.59 | -22.16* | -13.01 | -10.84 | 11.09 | -2.77 | -26.82 | -16.97 | -14.40 | 9.85 |
Cell Periphery | 3.67 | 1.58 | -3.60 | -6.64 | -5.21 | 0.28 | -4.46 | -5.00 | -5.99 | -1.22 | 0.73 | -3.80 | -7.11 | -8.76 | -5.08 |
Cytoplasm | -0.70 | -6.81 | -2.73 | -2.41 | 4.49 | 0.09 | -9.44 | -4.40 | -4.90 | 6.58 | -1.09 | -12.32 | -5.66 | -5.22 | 7.62 |
Cytoplasmic Foci | -1.16 | -0.37 | 2.02 | 6.38 | 8.64 | -4.10 | -4.74 | -1.61 | 2.70 | 3.06 | -6.55 | -8.55 | -0.24 | 6.56 | 8.72 |
Eisosomes | 4.65 | 5.04 | -1.70 | -6.97 | -7.36 | 3.69 | 6.45 | -3.76 | -4.82 | -5.46 | 5.27 | 7.87 | -6.36 | -8.36 | -9.13 |
Endoplasmic Reticulum | 5.02 | 4.98 | 4.12 | -3.35 | -3.12 | -2.21 | -3.77 | -4.62 | -3.86 | -2.55 | 2.04 | 0.24 | -3.82 | -5.17 | -4.06 |
Endosome | -0.18 | 1.94 | 4.51 | 7.44 | 9.52 | -5.74 | -6.76 | -6.01 | -0.55 | -1.26 | -8.27 | -10.40 | -3.58 | 5.82 | 6.31 |
Golgi | 5.49 | 7.54 | 7.96 | 9.06 | 4.38 | -3.78 | 1.70 | 2.09 | 5.30 | 0.33 | -3.36 | 4.89 | 7.06 | 10.33 | 3.51 |
Lipid Particles | 0.76 | 2.70 | 4.77 | 7.66 | 9.28 | -1.06 | 1.51 | -1.78 | -0.69 | -3.06 | -4.30 | -2.70 | 2.55 | 6.40 | 5.65 |
Mitochondria | -0.46 | -4.05 | 0.10 | 1.07 | 9.02 | 4.42 | -4.81 | 5.02 | 0.42 | 8.94 | 4.08 | -5.95 | 5.48 | 0.98 | 12.68 |
Mitotic Spindle | -1.55 | -8.32 | -3.01 | -1.71 | 5.13 | -1.03 | -6.66 | -4.47 | -2.90 | 5.01 | -1.93 | -10.77 | -4.93 | -3.20 | 7.28 |
None | 1.04 | 1.41 | 1.51 | 1.63 | 6.15 | 0.00 | -1.52 | 6.56 | 1.74 | 5.48 | -0.25 | 0.87 | 2.60 | 2.10 | 8.31 |
Nuclear Periphery | 4.23 | 1.71 | 4.50 | 0.78 | 5.97 | -0.77 | -5.75 | -4.20 | -1.54 | 3.67 | -0.52 | -8.25 | -2.35 | -1.00 | 6.84 |
Nuclear Periphery Foci | -1.22 | -1.20 | 1.71 | 1.92 | 2.58 | -1.46 | -5.02 | -2.70 | 0.41 | 1.49 | -2.64 | -5.22 | -2.82 | 1.56 | 2.99 |
Nucleolus | 0.50 | 1.01 | 1.23 | 1.27 | 3.45 | -0.96 | -2.71 | 0.58 | 1.01 | 3.21 | -1.19 | -1.42 | 1.33 | 1.44 | 4.79 |
Nucleus | 1.01 | 1.00 | 1.01 | 0.73 | 2.94 | -0.99 | -1.98 | -1.30 | 0.96 | 1.75 | 0.02 | 0.73 | 0.97 | 0.97 | 2.79 |
Peroxisomes | -7.51 | -11.22 | -0.70 | 7.84 | 13.31 | -4.08 | -2.90 | 4.20 | 7.04 | 6.61 | -6.00 | -7.43 | 5.60 | 10.63 | 14.29 |
Vacuole | 1.96 | -0.01 | 1.36 | -1.45 | 2.37 | 0.28 | -3.55 | -2.96 | -3.21 | 1.64 | 0.12 | -5.22 | -3.39 | -3.81 | 2.72 |
Vacuole Periphery | 4.75 | 2.00 | 1.02 | -1.64 | -0.45 | -0.20 | -3.14 | -1.58 | -1.50 | 0.48 | 0.19 | -2.79 | -2.16 | -2.24 | -0.17 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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