Standard name
Human Ortholog
Description Alpha catalytic subunit of casein kinase 2 (CK2); a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases; regulates Fkh1p-mediated donor preference during mating-type switching
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.05 0.05 0 0 0.05 0 0.05 0.05 0 0.05 0 0 0.19 0.18 0.2 0.06 0.06 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.07 0.07 0.07 0.1 0.06 0.13 0.08 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.62 0.59 0.74 0.69 0.76 0.65 0.66 0.63 0.65 0.6 0.61 0.67 0.6 0.61 0.61 0.71 0.6 0.57 0.79 0.77 0.64 0.46 0.35 0.34
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.08 0 0.05 0.28 0.09 0.16 0.14 0.21 0.17 0.16 0.17 0.13 0.08 0.07 0.08 0.08 0.08 0.11 0.06 0 0.18 0.15 0.12 0.13
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.32 0.44 0.21 0.07 0.15 0.13 0.13 0.11 0.15 0.18 0.15 0.16 0.14 0.16 0.18 0.19 0.21 0.3 0.08 0.11 0.11 0.27 0.41 0.4
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 0 0 1 0 0 0 0 0 0 0 1 0 1 0 0 2 0 0 0 0
Bud 0 0 0 0 1 2 2 2 0 7 2 1 0 0 0 0 1 0 8 11 5 10 9 7
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 2 3
Bud Site 0 0 0 0 0 6 4 4 5 7 11 3 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 5 14 11 9 20 19 31 27 17 28 13 18 27 44 50 9 9 4 1 3 2 2 0 4
Endoplasmic Reticulum 0 0 0 0 0 2 0 0 0 0 0 1 0 0 0 2 3 3 0 0 2 2 3 3
Endosome 0 2 1 0 2 4 5 6 2 5 2 7 2 1 5 1 0 0 0 5 3 7 11 11
Golgi 1 2 3 0 0 0 0 0 0 0 0 0 0 1 1 2 5 1 0 0 2 0 3 1
Mitochondria 1 1 0 2 1 41 41 40 43 36 60 47 1 0 0 2 6 5 0 1 0 1 2 4
Nucleus 69 174 205 189 316 357 395 364 290 336 270 384 84 149 155 108 90 80 122 287 229 132 137 152
Nuclear Periphery 0 0 0 1 1 1 0 3 1 2 3 6 0 0 0 0 0 0 0 1 1 0 5 11
Nucleolus 9 6 14 76 38 87 81 120 75 87 74 72 11 18 20 13 12 15 8 15 65 43 46 58
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 1 1 0 0 0 1 0 0 0 0 2 2 4 1 3
Vac/Vac Membrane 35 130 58 18 62 74 80 62 65 102 66 94 20 38 45 29 32 43 13 41 38 78 160 177
Unique Cell Count 111 297 278 272 418 551 595 578 443 558 445 574 139 243 256 153 149 141 156 376 357 287 391 446
Labelled Cell Count 120 329 293 295 441 593 640 628 498 611 502 633 146 251 278 166 159 151 156 376 357 287 391 446


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 19.2 17.8 18.3 17.0 14.7 11.8 11.7 11.3 11.3 12.0 10.4 10.5 17.1 17.8 18.4 19.8 19.8 19.1 15.7 16.7 17.5
Std Deviation (1e-4) 3.7 3.8 2.8 3.1 4.5 2.3 2.3 2.2 2.4 3.6 2.1 2.1 5.1 4.5 4.5 4.4 5.1 4.6 3.0 3.5 3.0
Intensity Change (Log2) – – – -0.11 -0.31 -0.63 -0.64 -0.7 -0.7 -0.61 -0.81 -0.8 -0.1 -0.03 0.01 0.12 0.12 0.07 -0.22 -0.13 -0.06


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.4 0.5 -0.4 0.8 0.5 -0.1 0.7 -0.8 -0.6 5.2 5.2 5.7 0.9 1.0 -0.6
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 4.7 4.5 4.5 5.4 4.3 6.4 4.9 0 0 0 0 0 0
Nucleus -1.1 0.6 -2.6 -2.2 -3.1 -2.3 -3.9 -3.6 -2.0 -2.8 -3.0 -3.2 -0.7 -2.8 -3.5
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 7.3 2.0 4.5 3.8 5.9 4.7 4.4 4.6 3.4 1.2 1.1 1.3 1.4 1.2 2.1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -4.8 -2.1 -2.8 -2.8 -4.0 -2.2 -0.9 -2.1 -1.6 -1.6 -1.5 -1.0 -0.5 0.1 2.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 14.482 14.7311 14.0579 12.8953 13.398 13.5779 16.2405 18.9131 17.462 17.2594 15.7293 17.462 15.1399 16.1688 14.3901 13.1721 12.7887 14.2609
Actin 0.0008 0 0 0 0 0.0001 0.0046 0 0.0002 0.0168 0.0008 0.0015 0.0289 0.008 0.0011 0.0345 0.0018 0.0044
Bud 0 0.0001 0 0 0.0001 0.0001 0.0006 0 0.0001 0.0001 0.0004 0.0001 0.0002 0.0001 0.0002 0.0004 0.0002 0.0011
Bud Neck 0.0001 0.0003 0.0004 0.0003 0.0008 0.0082 0.0011 0.0005 0.0005 0.0005 0.0055 0.0081 0.0005 0.0003 0.0008 0.0017 0.0002 0.0049
Bud Periphery 0.0001 0.0001 0.0001 0 0.0001 0.0003 0.001 0.0001 0.0003 0.0002 0.0006 0.0002 0.0002 0.0001 0.0001 0.0007 0.0002 0.0029
Bud Site 0.0001 0.0004 0.0002 0.0001 0.0002 0.0002 0.0014 0.0002 0.0004 0.0002 0.0025 0.0002 0.002 0.0006 0.0048 0.0016 0.0003 0.001
Cell Periphery 0.0001 0.0001 0.0002 0 0.0001 0.0003 0.0006 0.0001 0.0004 0.0002 0.0006 0.0002 0.0001 0.0002 0.0001 0.0005 0.0002 0.0005
Cytoplasm 0.0008 0.0012 0.0003 0.0003 0.0009 0.0009 0.003 0.0007 0.0009 0.0005 0.0051 0.0015 0.0005 0.0124 0.0078 0.0004 0.0079 0.0008
Cytoplasmic Foci 0.0024 0 0 0 0 0 0.0148 0 0.0036 0.0002 0.014 0.0013 0.0008 0.0008 0.0085 0.007 0.0143 0.0004
Eisosomes 0.0001 0 0 0 0 0 0.0001 0 0 0 0.0001 0 0.0001 0 0 0.0002 0.0001 0
Endoplasmic Reticulum 0.0003 0 0 0 0.0001 0.0001 0.0018 0 0.0005 0.0003 0.0022 0.0012 0.0001 0.0011 0.0009 0.0001 0.007 0.0025
Endosome 0.0013 0 0 0 0.0001 0.0002 0.0073 0 0.0027 0.0005 0.0115 0.0035 0.0006 0.0005 0.0132 0.0017 0.0203 0.0062
Golgi 0.0003 0 0 0 0 0 0.0018 0 0.0007 0.0002 0.0027 0.0008 0.0014 0.0004 0.0038 0.0051 0.0042 0.0011
Lipid Particles 0.0021 0 0 0 0 0 0.0147 0 0.0039 0.0001 0.0209 0.0012 0.0004 0.0001 0.0011 0.0064 0.032 0.0008
Mitochondria 0.0005 0.0001 0.0001 0.0001 0.0002 0.0001 0.0047 0.0001 0.0029 0.0005 0.0037 0.0005 0.0009 0.0005 0.0007 0.0029 0.0122 0.0028
None 0.0029 0 0 0 0 0 0.0019 0 0.0006 0 0.001 0.001 0 0.0002 0.0038 0.0003 0.0032 0.0003
Nuclear Periphery 0.0033 0.0028 0.002 0.0014 0.0044 0.0028 0.0092 0.0026 0.0053 0.0063 0.0085 0.0033 0.0027 0.002 0.0035 0.0025 0.0642 0.0103
Nucleolus 0.0244 0.0404 0.0483 0.0501 0.0242 0.0486 0.0384 0.0298 0.0572 0.0227 0.0287 0.0524 0.0102 0.0539 0.0266 0.0164 0.0179 0.0307
Nucleus 0.9588 0.9532 0.9473 0.947 0.9664 0.9329 0.8703 0.964 0.9133 0.9445 0.8658 0.9168 0.9493 0.9176 0.9181 0.8898 0.7857 0.9239
Peroxisomes 0.0002 0 0 0 0 0 0.005 0 0.0005 0.0004 0.0139 0.0002 0.0003 0.0001 0.0005 0.0214 0.0013 0.0002
Punctate Nuclear 0.0008 0.0004 0.0003 0.0002 0.0009 0.0026 0.0075 0.0005 0.0021 0.0006 0.0048 0.0039 0.0001 0.0002 0.0028 0.0016 0.0029 0.0006
Vacuole 0.0004 0.0007 0.0008 0.0003 0.0013 0.0023 0.0083 0.0012 0.0031 0.0033 0.0049 0.0019 0.0003 0.0008 0.0013 0.0042 0.0056 0.0032
Vacuole Periphery 0.0002 0.0001 0.0001 0.0001 0.0002 0.0002 0.002 0.0001 0.0008 0.0018 0.0017 0.0003 0.0002 0.0002 0.0004 0.0003 0.0185 0.0012

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 22.1864 17.6296 22.2393 41.6811 20.4556 25.6234 34.7593 36.6941 37.2091 44.1935
Translational Efficiency 1.9854 2.4273 1.9178 1.1825 1.9242 1.7022 1.4054 1.1542 1.1917 1.1141

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Alpha catalytic subunit of casein kinase 2 (CK2); a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases; regulates Fkh1p-mediated donor preference during mating-type switching
Localization
Cell Percentages nucleus (90%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-5

Cka1

Cka1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cka1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available