Standard name | |
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Human Ortholog | |
Description | Nuclear envelope/ER integral membrane protein; interacts and functions with Brr6p and Brl1p in lipid homeostasis; mutants are defective in nuclear pore complex biogenesis, nuclear envelope morphology, mRNA export from the nucleus and are sensitive to sterol biosynthesis inhibitors and membrane fluidizing agents; exhibits synthetic lethal genetic interactions with genes involved in lipid metabolism |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 16 | 9 | 27 | 27 | 23 | 9 | – | 1 | 1 | 0 | 1 | 2 | 13 | 21 | 19 | 11 | 7 | 8 | 11 | 9 | 13 | 5 | 8 | 10 |
Bud | 7 | 3 | 12 | 5 | 10 | 12 | – | 10 | 11 | 21 | 7 | 23 | 1 | 4 | 4 | 0 | 0 | 0 | 5 | 3 | 3 | 1 | 4 | 7 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 2 | 2 | 1 | 0 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 1 | – | 1 | 0 | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 12 | 15 | 23 | 32 | 41 | 29 | – | 23 | 10 | 12 | 11 | 15 | 43 | 86 | 89 | 33 | 5 | 28 | 2 | 2 | 6 | 3 | 6 | 8 |
Cytoplasm | 56 | 44 | 59 | 45 | 95 | 111 | – | 158 | 133 | 196 | 104 | 157 | 55 | 129 | 130 | 46 | 3 | 18 | 13 | 8 | 5 | 7 | 11 | 22 |
Endoplasmic Reticulum | 140 | 153 | 191 | 191 | 183 | 102 | – | 37 | 35 | 27 | 39 | 21 | 201 | 220 | 221 | 2 | 0 | 1 | 216 | 181 | 284 | 139 | 221 | 210 |
Endosome | 2 | 0 | 2 | 0 | 16 | 11 | – | 8 | 2 | 8 | 2 | 5 | 1 | 3 | 0 | 3 | 0 | 0 | 2 | 0 | 3 | 1 | 0 | 3 |
Golgi | 0 | 1 | 0 | 0 | 3 | 0 | – | 0 | 0 | 0 | 0 | 0 | 5 | 2 | 3 | 13 | 0 | 6 | 1 | 1 | 2 | 0 | 0 | 3 |
Mitochondria | 5 | 0 | 76 | 130 | 68 | 138 | – | 237 | 234 | 250 | 180 | 279 | 7 | 14 | 7 | 6 | 2 | 4 | 8 | 0 | 12 | 9 | 7 | 5 |
Nucleus | 0 | 1 | 2 | 0 | 1 | 1 | – | 10 | 7 | 6 | 5 | 8 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 5 | 1 | 0 | 3 | – | 7 | 12 | 3 | 7 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 1 | 1 | 3 |
Nucleolus | 0 | 0 | 0 | 1 | 2 | 0 | – | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 1 | – | 1 | 0 | 2 | 0 | 3 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 |
Vac/Vac Membrane | 34 | 7 | 18 | 4 | 40 | 27 | – | 98 | 51 | 54 | 18 | 35 | 14 | 16 | 34 | 41 | 3 | 15 | 14 | 3 | 6 | 5 | 12 | 13 |
Unique Cell Count | 231 | 204 | 314 | 280 | 377 | 324 | 450 | 384 | 466 | 283 | 426 | 275 | 392 | 389 | 123 | 18 | 63 | 284 | 217 | 346 | 180 | 280 | 296 | |
Labelled Cell Count | 272 | 233 | 415 | 436 | 483 | 445 | 591 | 496 | 580 | 375 | 555 | 343 | 497 | 508 | 155 | 20 | 80 | 284 | 217 | 346 | 180 | 280 | 296 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 9.4 | 11.3 | 7.3 | 8.7 | 8.6 | 6.4 | – | 5.5 | 4.7 | 4.8 | 4.4 | 4.3 | 11.0 | 10.4 | 9.6 | 13.0 | 11.3 | 12.4 | 10.4 | 10.7 | 10.5 |
Std Deviation (1e-4) | 7.4 | 2.2 | 2.0 | 2.3 | 2.0 | 1.8 | – | 1.5 | 1.3 | 1.3 | 1.3 | 1.5 | 2.5 | 2.3 | 2.3 | 2.7 | 2.0 | 2.4 | 2.6 | 2.5 | 2.5 |
Intensity Change (Log2) | – | – | – | 0.25 | 0.23 | -0.19 | – | -0.42 | -0.65 | -0.6 | -0.72 | -0.77 | 0.59 | 0.51 | 0.39 | 0.83 | 0.63 | 0.77 | 0.51 | 0.55 | 0.53 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0.4 | -1.3 | -3.2 | – | -6.1 | 0 | 0 | 0 | 0 | -1.9 | -1.7 | -2.0 | 0.1 | 0 | 1.0 |
Bud | -1.5 | -0.9 | -0.1 | – | -1.3 | 0 | 0 | 0 | 0 | -2.8 | -2.5 | -2.5 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 1.7 | 1.6 | 0.8 | – | -1.3 | 0 | 0 | 0 | 0 | 3.2 | 5.3 | 5.6 | 5.5 | 0 | 7.9 |
Cytoplasm | -0.9 | 2.0 | 4.4 | – | 4.9 | 4.7 | 6.8 | 4.9 | 5.3 | 0.4 | 4.2 | 4.3 | 4.1 | 0 | 1.8 |
Endoplasmic Reticulum | 1.9 | -3.2 | -7.4 | – | -15.6 | 0 | 0 | 0 | 0 | 3.1 | -1.3 | -1.1 | -11.2 | -5.1 | -8.6 |
Endosome | 0 | 3.0 | 2.5 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 5.7 | -2.0 | 4.9 | – | 7.9 | 0 | 0 | 0 | 0 | -7.5 | -8.2 | -9.2 | -4.7 | 0 | -3.2 |
Nucleus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -2.8 | 2.3 | 1.3 | – | 6.1 | 3.3 | 2.8 | 0.3 | 1.3 | -0.3 | -1.0 | 1.5 | 7.6 | 0 | 4.6 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 6.5082 | 7.1259 | 6.4581 | 6.1674 | 5.2537 | 6.9134 | 6.6798 | 6.7492 | 6.6159 | 5.5648 | 5.8501 | 6.717 | 9.6121 | 10.8913 | 10.1089 | 7.6604 | 10.4298 | 9.3542 |
Actin | 0.0146 | 0.0044 | 0.0134 | 0.0339 | 0.0081 | 0.0028 | 0.0221 | 0.0018 | 0.0121 | 0.0265 | 0.0269 | 0.0007 | 0.0075 | 0.0021 | 0.01 | 0.0121 | 0.0136 | 0.0085 |
Bud | 0.0003 | 0.0001 | 0.0004 | 0.0007 | 0.0007 | 0 | 0.001 | 0.0001 | 0.0004 | 0.0004 | 0.0013 | 0 | 0.0037 | 0.0007 | 0.0028 | 0.0003 | 0.0002 | 0.0001 |
Bud Neck | 0.0028 | 0.0003 | 0.0103 | 0.0199 | 0.0009 | 0.0009 | 0.008 | 0.0012 | 0.0004 | 0.0063 | 0.0026 | 0.0004 | 0.0007 | 0.0021 | 0.0039 | 0.0007 | 0.0061 | 0.0082 |
Bud Periphery | 0.0004 | 0.0001 | 0.0007 | 0.0015 | 0.0013 | 0.0001 | 0.0011 | 0.0002 | 0.0007 | 0.001 | 0.0023 | 0.0002 | 0.01 | 0.0013 | 0.008 | 0.0028 | 0.0013 | 0.001 |
Bud Site | 0.0075 | 0.0043 | 0.0054 | 0.0014 | 0.0011 | 0.0001 | 0.0084 | 0.0115 | 0.0022 | 0.001 | 0.0051 | 0.0001 | 0.0013 | 0.0171 | 0.0027 | 0.0002 | 0.0002 | 0.0001 |
Cell Periphery | 0.0018 | 0.0029 | 0.0028 | 0.0032 | 0.0007 | 0.0006 | 0.001 | 0.0085 | 0.0012 | 0.0047 | 0.0023 | 0.0039 | 0.0078 | 0.0346 | 0.0224 | 0.0127 | 0.0307 | 0.0149 |
Cytoplasm | 0.0672 | 0.0828 | 0.0636 | 0.0568 | 0.0277 | 0.0441 | 0.046 | 0.1134 | 0.0307 | 0.0713 | 0.0179 | 0.0329 | 0.0759 | 0.1232 | 0.1079 | 0.0562 | 0.1055 | 0.07 |
Cytoplasmic Foci | 0.018 | 0.0023 | 0.0121 | 0.0328 | 0.0307 | 0.0037 | 0.0165 | 0.0065 | 0.0083 | 0.008 | 0.0239 | 0.0023 | 0.0173 | 0.008 | 0.0074 | 0.0042 | 0.0017 | 0.0016 |
Eisosomes | 0.0003 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0 | 0.0001 | 0.0001 | 0.0002 | 0.0003 | 0.0213 | 0.0011 |
Endoplasmic Reticulum | 0.7804 | 0.8756 | 0.8146 | 0.6793 | 0.6009 | 0.9038 | 0.7502 | 0.8182 | 0.8826 | 0.7624 | 0.6724 | 0.9305 | 0.7853 | 0.7517 | 0.7734 | 0.8657 | 0.7865 | 0.8612 |
Endosome | 0.0268 | 0.0037 | 0.0342 | 0.0946 | 0.1075 | 0.0064 | 0.0491 | 0.0087 | 0.0127 | 0.0373 | 0.0975 | 0.0059 | 0.0244 | 0.0217 | 0.019 | 0.008 | 0.0031 | 0.0058 |
Golgi | 0.0068 | 0.002 | 0.0112 | 0.0235 | 0.0476 | 0.0022 | 0.0166 | 0.0013 | 0.0065 | 0.0195 | 0.0272 | 0.0015 | 0.0046 | 0.0033 | 0.0061 | 0.0068 | 0.0057 | 0.0024 |
Lipid Particles | 0.0196 | 0.0059 | 0.0095 | 0.0132 | 0.0312 | 0.0084 | 0.0207 | 0.0107 | 0.0051 | 0.033 | 0.0128 | 0.0029 | 0.0134 | 0.0136 | 0.0141 | 0.0155 | 0.0131 | 0.0022 |
Mitochondria | 0.002 | 0.0038 | 0.0054 | 0.0099 | 0.0875 | 0.0068 | 0.015 | 0.0004 | 0.01 | 0.0031 | 0.0145 | 0.0032 | 0.0027 | 0.0005 | 0.001 | 0.0004 | 0.0001 | 0.0002 |
None | 0.0139 | 0.0008 | 0.0011 | 0.001 | 0.001 | 0.0025 | 0.0077 | 0.0013 | 0.0041 | 0.0008 | 0.002 | 0.0003 | 0.0028 | 0.0016 | 0.0014 | 0.0005 | 0.0021 | 0.0016 |
Nuclear Periphery | 0.0133 | 0.0057 | 0.0064 | 0.0087 | 0.0039 | 0.01 | 0.0161 | 0.0061 | 0.0087 | 0.0146 | 0.0337 | 0.0056 | 0.0161 | 0.0084 | 0.0106 | 0.0073 | 0.0046 | 0.0132 |
Nucleolus | 0.0019 | 0 | 0.0001 | 0.0001 | 0.0002 | 0 | 0.0007 | 0.0002 | 0.0002 | 0.0001 | 0.001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0002 | 0.0001 |
Nucleus | 0.0026 | 0.0007 | 0.0007 | 0.0009 | 0.0009 | 0.0011 | 0.0032 | 0.002 | 0.0013 | 0.0017 | 0.0214 | 0.0006 | 0.0022 | 0.0015 | 0.0011 | 0.0006 | 0.0007 | 0.0032 |
Peroxisomes | 0.008 | 0.001 | 0.0024 | 0.009 | 0.0331 | 0.0024 | 0.0046 | 0.0001 | 0.0057 | 0.0005 | 0.0035 | 0.006 | 0.0138 | 0.0001 | 0.0009 | 0.0002 | 0.0001 | 0.0001 |
Punctate Nuclear | 0.0056 | 0.0002 | 0.0005 | 0.0012 | 0.0008 | 0.0017 | 0.0053 | 0.0014 | 0.0031 | 0.0008 | 0.0046 | 0.0002 | 0.0034 | 0.0006 | 0.001 | 0.0002 | 0.0003 | 0.0019 |
Vacuole | 0.0053 | 0.0028 | 0.004 | 0.006 | 0.0091 | 0.0019 | 0.0049 | 0.0059 | 0.0033 | 0.0059 | 0.0145 | 0.0023 | 0.0059 | 0.0069 | 0.0055 | 0.0047 | 0.0029 | 0.0024 |
Vacuole Periphery | 0.0009 | 0.0002 | 0.0009 | 0.0023 | 0.0049 | 0.0004 | 0.0016 | 0.0004 | 0.0007 | 0.001 | 0.0123 | 0.0004 | 0.001 | 0.0006 | 0.0008 | 0.0007 | 0.0002 | 0.0003 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 48.952 | 21.2887 | 28.0838 | 37.3228 | 42.8841 | 23.8013 | 35.277 | 39.5482 | 25.6995 | 48.393 |
Translational Efficiency | 0.7768 | 1.3801 | 1.2827 | 0.6179 | 0.7933 | 2.0422 | 1.2108 | 0.9292 | 1.7292 | 1.327 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1792 | 825 | 1242 | 365 | 811 | 1850 | 2753 | 121 | 2603 | 2675 | 3995 | 486 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 907.52 | 1058.53 | 1460.12 | 1234.16 | 826.26 | 1103.27 | 1383.12 | 1218.44 | 882.20 | 1089.47 | 1407.06 | 1230.25 |
Standard Deviation | 135.56 | 196.01 | 262.25 | 220.28 | 1085.27 | 183.52 | 237.89 | 203.95 | 617.28 | 188.60 | 248.29 | 216.44 |
Intensity Change Log 2 | — | 0.222061 | 0.686086 | 0.443528 | — | 0.417118 | 0.743259 | 0.560367 | — | 0.318312 | 0.713615 | 0.500391 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.002001 | 0.005536 | 0.002679 | 0.046358 | 0.002214 | 0.001832 | 0.005972 | 0.048917 | 0.002067 | 0.002974 | 0.004948 | 0.046995 |
Bud Neck | 0.001858 | 0.002716 | 0.002328 | 0.024183 | 0.005509 | 0.001509 | 0.005322 | 0.015581 | 0.002996 | 0.001881 | 0.004391 | 0.022041 |
Bud Site | 0.009244 | 0.018591 | 0.011711 | 0.121058* | 0.026636 | 0.010315 | 0.021096 | 0.063273 | 0.014662 | 0.012868 | 0.018179 | 0.106671* |
Cell Periphery | 0.006228 | 0.009333 | 0.013668 | 0.030106 | 0.017534 | 0.009761 | 0.017368 | 0.020653 | 0.009751 | 0.009629 | 0.016218 | 0.027752 |
Cytoplasm | 0.117630* | 0.068313 | 0.020496 | 0.086003 | 0.209550* | 0.074111 | 0.050148 | 0.074044 | 0.146269* | 0.072323 | 0.040929 | 0.083026 |
Cytoplasmic Foci | 0.022879 | 0.037636 | 0.001199 | 0.046174 | 0.061984 | 0.013298 | 0.004229 | 0.025938 | 0.035063 | 0.020805 | 0.003287 | 0.041136 |
Eisosomes | 0.001583 | 0.001335 | 0.000338 | 0.005293 | 0.002690 | 0.001213 | 0.001288 | 0.006951 | 0.001928 | 0.001250 | 0.000992 | 0.005706 |
Endoplasmic Reticulum | 0.744304* | 0.756659* | 0.919447* | 0.351995* | 0.422761* | 0.827757* | 0.830397* | 0.463709* | 0.644123* | 0.805830* | 0.858082* | 0.379809* |
Endosome | 0.006222 | 0.013707 | 0.004674 | 0.034400 | 0.008689 | 0.006039 | 0.007260 | 0.023957 | 0.006991 | 0.008404 | 0.006456 | 0.031800 |
Golgi | 0.018104 | 0.019305 | 0.001474 | 0.012515 | 0.022882 | 0.011925 | 0.002958 | 0.016375 | 0.019593 | 0.014202 | 0.002497 | 0.013476 |
Lipid Particles | 0.028779 | 0.020708 | 0.008138 | 0.111293* | 0.061100 | 0.015212 | 0.022921 | 0.153211* | 0.038849 | 0.016907 | 0.018325 | 0.121729* |
Mitochondria | 0.009148 | 0.006408 | 0.000311 | 0.004814 | 0.072195 | 0.004995 | 0.002535 | 0.000426 | 0.028791 | 0.005431 | 0.001844 | 0.003722 |
Mitotic Spindle | 0.000068 | 0.005072 | 0.000164 | 0.018757 | 0.000207 | 0.000880 | 0.000557 | 0.002098 | 0.000112 | 0.002173 | 0.000434 | 0.014610 |
None | 0.005431 | 0.002214 | 0.000585 | 0.000060 | 0.006941 | 0.003399 | 0.004055 | 0.000081 | 0.005901 | 0.003034 | 0.002976 | 0.000065 |
Nuclear Periphery | 0.000471 | 0.000551 | 0.000396 | 0.000733 | 0.002946 | 0.000146 | 0.000430 | 0.000343 | 0.001242 | 0.000271 | 0.000419 | 0.000636 |
Nuclear Periphery Foci | 0.002791 | 0.005752 | 0.003716 | 0.016536 | 0.002508 | 0.001192 | 0.004476 | 0.015207 | 0.002703 | 0.002599 | 0.004240 | 0.016205 |
Nucleolus | 0.000090 | 0.000174 | 0.000012 | 0.000059 | 0.001188 | 0.000037 | 0.000055 | 0.000393 | 0.000432 | 0.000079 | 0.000042 | 0.000142 |
Nucleus | 0.003792 | 0.002458 | 0.001262 | 0.004533 | 0.036541 | 0.002305 | 0.004765 | 0.004950 | 0.013995 | 0.002352 | 0.003676 | 0.004637 |
Peroxisomes | 0.014243 | 0.005991 | 0.000480 | 0.004466 | 0.021850 | 0.009780 | 0.003652 | 0.006857 | 0.016613 | 0.008611 | 0.002666 | 0.005061 |
Vacuole | 0.003532 | 0.012564 | 0.006671 | 0.076337 | 0.008406 | 0.003053 | 0.010107 | 0.056186 | 0.005051 | 0.005986 | 0.009039 | 0.071320 |
Vacuole Periphery | 0.001602 | 0.004976 | 0.000253 | 0.004328 | 0.005672 | 0.001239 | 0.000410 | 0.000850 | 0.002870 | 0.002391 | 0.000361 | 0.003462 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -2.44 | -0.98 | -7.35 | -6.56 | -7.20 | 1.05 | -5.33 | -3.77 | -3.81 | -3.43 | -1.71 | -5.21 | -8.22 | -8.02 | -7.61 |
Bud Neck | -1.18 | -1.17 | -7.96 | -7.49 | -7.75 | 6.41 | 0.05 | -2.92 | -4.07 | -2.93 | 2.76 | -3.01 | -8.35 | -8.84 | -7.61 |
Bud Site | -3.41 | -1.27 | -11.35 | -10.15 | -10.98 | 4.49 | 1.19 | -2.99 | -4.47 | -3.46 | 0.96 | -2.31 | -11.37 | -11.62 | -10.86 |
Cell Periphery | -1.88 | -4.70 | -5.58 | -4.50 | -3.47 | 3.30 | 0.08 | -0.43 | -2.03 | -0.50 | 0.10 | -5.47 | -5.01 | -5.06 | -3.02 |
Cytoplasm | 8.47 | 23.12 | 2.92 | -2.47 | -8.39 | 16.78 | 20.38 | 7.83 | -0.42 | -1.91 | 16.91 | 26.78 | 7.23 | -2.16 | -6.34 |
Cytoplasmic Foci | -3.86 | 14.68 | -5.00 | -1.79 | -9.60 | 13.50 | 16.75 | 5.74 | -2.57 | -4.38 | 6.95 | 20.56 | -1.92 | -5.34 | -10.07 |
Eisosomes | 0.55 | 5.58 | -2.26 | -2.37 | -3.11 | 3.81 | 3.59 | -1.81 | -2.50 | -2.46 | 2.76 | 4.33 | -2.77 | -3.31 | -3.53 |
Endoplasmic Reticulum | -0.86 | -19.75 | 19.72 | 18.62 | 28.60 | -28.17* | -28.69 | -0.69 | 10.46 | 10.43 | -18.26 | -26.25 | 15.13 | 24.34 | 27.56 |
Endosome | -6.39 | 3.06 | -7.61 | -5.28 | -8.05 | 2.95 | 1.47 | -3.78 | -4.51 | -4.16 | -2.35 | 0.89 | -8.38 | -7.84 | -8.54 |
Golgi | -0.51 | 14.78 | 2.89 | 2.69 | -5.22 | 5.50 | 12.29 | 1.68 | -0.90 | -3.20 | 3.88 | 18.30 | 3.50 | 0.76 | -5.74 |
Lipid Particles | 3.03 | 10.55 | -8.20 | -8.94 | -10.38 | 11.30 | 9.07 | -4.02 | -6.13 | -5.77 | 11.29 | 10.24 | -8.70 | -11.17 | -10.99 |
Mitochondria | 1.82 | 11.83 | 2.27 | 0.83 | -2.16 | 12.26 | 12.76 | 13.16 | 7.91 | 7.64 | 12.00 | 14.40 | 10.72 | 1.27 | -1.10 |
Mitotic Spindle | -2.68 | -1.75 | -3.91 | -2.87 | -3.90 | -1.30 | -1.80 | -3.00 | -1.67 | -2.37 | -3.03 | -2.39 | -4.02 | -3.49 | -3.94 |
None | 2.61 | 5.60 | 6.75 | 2.27 | 1.52 | 2.22 | 1.92 | 4.55 | 3.70 | 5.24 | 3.07 | 3.48 | 8.11 | 4.55 | 5.81 |
Nuclear Periphery | -0.36 | 0.47 | -0.96 | -0.67 | -1.31 | 3.99 | 3.57 | 3.62 | -3.04 | 0.59 | 3.95 | 3.37 | 1.77 | -1.88 | -1.17 |
Nuclear Periphery Foci | -1.70 | -1.65 | -4.51 | -3.30 | -4.25 | 3.66 | -3.43 | -3.98 | -4.36 | -3.38 | 0.16 | -3.31 | -5.59 | -5.57 | -4.98 |
Nucleolus | -1.03 | 6.87 | 1.00 | 1.22 | -4.87 | 2.27 | 2.24 | 1.37 | -1.00 | -0.94 | 2.20 | 2.44 | 1.61 | -0.78 | -1.23 |
Nucleus | 1.90 | 2.72 | -2.01 | -3.36 | -3.96 | 7.91 | 7.24 | 6.85 | -2.20 | -0.21 | 8.12 | 6.80 | 5.60 | -4.52 | -1.75 |
Peroxisomes | 6.23 | 12.10 | 7.56 | 1.59 | -5.10 | 5.97 | 9.98 | 6.22 | 1.64 | -1.80 | 6.82 | 14.16 | 10.08 | 3.85 | -3.13 |
Vacuole | -4.80 | -4.60 | -11.00 | -9.64 | -10.46 | 5.22 | -1.97 | -4.19 | -4.60 | -4.01 | -1.24 | -6.75 | -11.57 | -11.41 | -10.85 |
Vacuole Periphery | -4.00 | 9.36 | -1.74 | 0.59 | -2.72 | 8.08 | 10.03 | 7.85 | 1.10 | -1.25 | 1.38 | 12.79 | -0.34 | -0.77 | -2.74 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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