Standard name
Human Ortholog
Description Nuclear envelope/ER integral membrane protein; interacts and functions with Brr6p and Brl1p in lipid homeostasis; mutants are defective in nuclear pore complex biogenesis, nuclear envelope morphology, mRNA export from the nucleus and are sensitive to sterol biosynthesis inhibitors and membrane fluidizing agents; exhibits synthetic lethal genetic interactions with genes involved in lipid metabolism
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.07 0 0.09 0.1 0.06 0 – 0 0 0 0 0 0.05 0.05 0.05 0.09 0.39 0.13 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0.05 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.05 0.07 0.07 0.11 0.11 0.09 – 0.05 0 0 0 0 0.16 0.22 0.23 0.27 0.28 0.44 0 0 0 0 0 0
Cytoplasm 0.24 0.22 0.19 0.16 0.25 0.34 – 0.35 0.35 0.42 0.37 0.37 0.2 0.33 0.33 0.37 0.17 0.29 0.05 0 0 0 0 0.08
Endoplasmic Reticulum 0.61 0.75 0.61 0.68 0.49 0.31 – 0.08 0.09 0.06 0.14 0.05 0.73 0.56 0.57 0 0 0 0.76 0.84 0.82 0.77 0.79 0.71
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.11 0 0.1 0 0 0 0 0 0
Mitochondria 0 0 0.24 0.46 0.18 0.43 – 0.53 0.61 0.54 0.64 0.65 0 0 0 0.05 0.11 0.06 0 0 0 0.05 0 0
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.15 0 0.06 0 0.11 0.08 – 0.22 0.13 0.12 0.06 0.08 0.05 0 0.09 0.33 0.17 0.24 0.05 0 0 0 0 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 16 9 27 27 23 9 – 1 1 0 1 2 13 21 19 11 7 8 11 9 13 5 8 10
Bud 7 3 12 5 10 12 – 10 11 21 7 23 1 4 4 0 0 0 5 3 3 1 4 7
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 3 2 2 1 0 1
Bud Site 0 0 0 0 0 1 – 1 0 0 0 3 0 0 0 0 0 0 – – – – – –
Cell Periphery 12 15 23 32 41 29 – 23 10 12 11 15 43 86 89 33 5 28 2 2 6 3 6 8
Cytoplasm 56 44 59 45 95 111 – 158 133 196 104 157 55 129 130 46 3 18 13 8 5 7 11 22
Endoplasmic Reticulum 140 153 191 191 183 102 – 37 35 27 39 21 201 220 221 2 0 1 216 181 284 139 221 210
Endosome 2 0 2 0 16 11 – 8 2 8 2 5 1 3 0 3 0 0 2 0 3 1 0 3
Golgi 0 1 0 0 3 0 – 0 0 0 0 0 5 2 3 13 0 6 1 1 2 0 0 3
Mitochondria 5 0 76 130 68 138 – 237 234 250 180 279 7 14 7 6 2 4 8 0 12 9 7 5
Nucleus 0 1 2 0 1 1 – 10 7 6 5 8 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 5 1 0 3 – 7 12 3 7 3 1 0 0 0 0 0 2 0 1 1 1 3
Nucleolus 0 0 0 1 2 0 – 0 0 1 1 0 1 0 1 0 0 0 0 0 0 1 0 0
Peroxisomes 0 0 0 0 1 0 – 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 1 – 1 0 2 0 3 1 1 0 0 0 0 0 0 0 0 1 2
Vac/Vac Membrane 34 7 18 4 40 27 – 98 51 54 18 35 14 16 34 41 3 15 14 3 6 5 12 13
Unique Cell Count 231 204 314 280 377 324 450 384 466 283 426 275 392 389 123 18 63 284 217 346 180 280 296
Labelled Cell Count 272 233 415 436 483 445 591 496 580 375 555 343 497 508 155 20 80 284 217 346 180 280 296


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.4 11.3 7.3 8.7 8.6 6.4 – 5.5 4.7 4.8 4.4 4.3 11.0 10.4 9.6 13.0 11.3 12.4 10.4 10.7 10.5
Std Deviation (1e-4) 7.4 2.2 2.0 2.3 2.0 1.8 – 1.5 1.3 1.3 1.3 1.5 2.5 2.3 2.3 2.7 2.0 2.4 2.6 2.5 2.5
Intensity Change (Log2) – – – 0.25 0.23 -0.19 – -0.42 -0.65 -0.6 -0.72 -0.77 0.59 0.51 0.39 0.83 0.63 0.77 0.51 0.55 0.53


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0.4 -1.3 -3.2 – -6.1 0 0 0 0 -1.9 -1.7 -2.0 0.1 0 1.0
Bud -1.5 -0.9 -0.1 – -1.3 0 0 0 0 -2.8 -2.5 -2.5 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 1.7 1.6 0.8 – -1.3 0 0 0 0 3.2 5.3 5.6 5.5 0 7.9
Cytoplasm -0.9 2.0 4.4 – 4.9 4.7 6.8 4.9 5.3 0.4 4.2 4.3 4.1 0 1.8
Endoplasmic Reticulum 1.9 -3.2 -7.4 – -15.6 0 0 0 0 3.1 -1.3 -1.1 -11.2 -5.1 -8.6
Endosome 0 3.0 2.5 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 5.7 -2.0 4.9 – 7.9 0 0 0 0 -7.5 -8.2 -9.2 -4.7 0 -3.2
Nucleus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vacuole -2.8 2.3 1.3 – 6.1 3.3 2.8 0.3 1.3 -0.3 -1.0 1.5 7.6 0 4.6

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 6.5082 7.1259 6.4581 6.1674 5.2537 6.9134 6.6798 6.7492 6.6159 5.5648 5.8501 6.717 9.6121 10.8913 10.1089 7.6604 10.4298 9.3542
Actin 0.0146 0.0044 0.0134 0.0339 0.0081 0.0028 0.0221 0.0018 0.0121 0.0265 0.0269 0.0007 0.0075 0.0021 0.01 0.0121 0.0136 0.0085
Bud 0.0003 0.0001 0.0004 0.0007 0.0007 0 0.001 0.0001 0.0004 0.0004 0.0013 0 0.0037 0.0007 0.0028 0.0003 0.0002 0.0001
Bud Neck 0.0028 0.0003 0.0103 0.0199 0.0009 0.0009 0.008 0.0012 0.0004 0.0063 0.0026 0.0004 0.0007 0.0021 0.0039 0.0007 0.0061 0.0082
Bud Periphery 0.0004 0.0001 0.0007 0.0015 0.0013 0.0001 0.0011 0.0002 0.0007 0.001 0.0023 0.0002 0.01 0.0013 0.008 0.0028 0.0013 0.001
Bud Site 0.0075 0.0043 0.0054 0.0014 0.0011 0.0001 0.0084 0.0115 0.0022 0.001 0.0051 0.0001 0.0013 0.0171 0.0027 0.0002 0.0002 0.0001
Cell Periphery 0.0018 0.0029 0.0028 0.0032 0.0007 0.0006 0.001 0.0085 0.0012 0.0047 0.0023 0.0039 0.0078 0.0346 0.0224 0.0127 0.0307 0.0149
Cytoplasm 0.0672 0.0828 0.0636 0.0568 0.0277 0.0441 0.046 0.1134 0.0307 0.0713 0.0179 0.0329 0.0759 0.1232 0.1079 0.0562 0.1055 0.07
Cytoplasmic Foci 0.018 0.0023 0.0121 0.0328 0.0307 0.0037 0.0165 0.0065 0.0083 0.008 0.0239 0.0023 0.0173 0.008 0.0074 0.0042 0.0017 0.0016
Eisosomes 0.0003 0.0001 0.0002 0.0001 0.0002 0 0.0003 0.0001 0.0001 0.0001 0.0003 0 0.0001 0.0001 0.0002 0.0003 0.0213 0.0011
Endoplasmic Reticulum 0.7804 0.8756 0.8146 0.6793 0.6009 0.9038 0.7502 0.8182 0.8826 0.7624 0.6724 0.9305 0.7853 0.7517 0.7734 0.8657 0.7865 0.8612
Endosome 0.0268 0.0037 0.0342 0.0946 0.1075 0.0064 0.0491 0.0087 0.0127 0.0373 0.0975 0.0059 0.0244 0.0217 0.019 0.008 0.0031 0.0058
Golgi 0.0068 0.002 0.0112 0.0235 0.0476 0.0022 0.0166 0.0013 0.0065 0.0195 0.0272 0.0015 0.0046 0.0033 0.0061 0.0068 0.0057 0.0024
Lipid Particles 0.0196 0.0059 0.0095 0.0132 0.0312 0.0084 0.0207 0.0107 0.0051 0.033 0.0128 0.0029 0.0134 0.0136 0.0141 0.0155 0.0131 0.0022
Mitochondria 0.002 0.0038 0.0054 0.0099 0.0875 0.0068 0.015 0.0004 0.01 0.0031 0.0145 0.0032 0.0027 0.0005 0.001 0.0004 0.0001 0.0002
None 0.0139 0.0008 0.0011 0.001 0.001 0.0025 0.0077 0.0013 0.0041 0.0008 0.002 0.0003 0.0028 0.0016 0.0014 0.0005 0.0021 0.0016
Nuclear Periphery 0.0133 0.0057 0.0064 0.0087 0.0039 0.01 0.0161 0.0061 0.0087 0.0146 0.0337 0.0056 0.0161 0.0084 0.0106 0.0073 0.0046 0.0132
Nucleolus 0.0019 0 0.0001 0.0001 0.0002 0 0.0007 0.0002 0.0002 0.0001 0.001 0.0001 0.0001 0.0001 0.0001 0 0.0002 0.0001
Nucleus 0.0026 0.0007 0.0007 0.0009 0.0009 0.0011 0.0032 0.002 0.0013 0.0017 0.0214 0.0006 0.0022 0.0015 0.0011 0.0006 0.0007 0.0032
Peroxisomes 0.008 0.001 0.0024 0.009 0.0331 0.0024 0.0046 0.0001 0.0057 0.0005 0.0035 0.006 0.0138 0.0001 0.0009 0.0002 0.0001 0.0001
Punctate Nuclear 0.0056 0.0002 0.0005 0.0012 0.0008 0.0017 0.0053 0.0014 0.0031 0.0008 0.0046 0.0002 0.0034 0.0006 0.001 0.0002 0.0003 0.0019
Vacuole 0.0053 0.0028 0.004 0.006 0.0091 0.0019 0.0049 0.0059 0.0033 0.0059 0.0145 0.0023 0.0059 0.0069 0.0055 0.0047 0.0029 0.0024
Vacuole Periphery 0.0009 0.0002 0.0009 0.0023 0.0049 0.0004 0.0016 0.0004 0.0007 0.001 0.0123 0.0004 0.001 0.0006 0.0008 0.0007 0.0002 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 48.952 21.2887 28.0838 37.3228 42.8841 23.8013 35.277 39.5482 25.6995 48.393
Translational Efficiency 0.7768 1.3801 1.2827 0.6179 0.7933 2.0422 1.2108 0.9292 1.7292 1.327

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Nuclear envelope/ER integral membrane protein; interacts and functions with Brr6p and Brl1p in lipid homeostasis; mutants are defective in nuclear pore complex biogenesis, nuclear envelope morphology, mRNA export from the nucleus and are sensitive to sterol biosynthesis inhibitors and membrane fluidizing agents; exhibits synthetic lethal genetic interactions with genes involved in lipid metabolism
Localization
Cell Percentages ER (95%)
Cell Cycle Regulation No
Subcompartmental Group ER-3

Apq12

Apq12


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Apq12-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available