Standard name
Human Ortholog
Description Putative type-1 protein phosphatase targeting subunit; tethers Glc7p type-1 protein phosphatase to Gsy2p glycogen synthase; PIG2 has a paralog, GIP2, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.82 0.98 0.98 0.98 0.97 0.89 0.86 0.88 0.82 0.83 0.78 0.82 0.99 0.98 1.0 0.92 0.9 0.98 0.69 0.93 0.79 0.94 0.93 0.9
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.18 0 0.05 0.09 0.06 0.23 0.31 0.19 0.31 0.3 0.4 0.34 0 0 0 0.06 0.05 0 0.12 0 0.08 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 4 1 2 0 0 0
Bud 1 2 3 1 3 3 2 4 5 0 3 2 0 0 1 1 0 0 1 0 0 1 1 0
Bud Neck 0 1 4 1 6 1 6 5 2 4 1 1 0 0 0 1 0 2 1 0 1 0 0 0
Bud Site 0 0 0 0 0 1 1 1 2 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 0 2 1 2 2 1 2 5 2 1 3 2 2 6 0 1 1 0 0 1 0 1 0
Cytoplasm 59 262 213 170 270 220 199 229 206 210 90 111 280 256 313 77 86 46 159 251 153 173 288 162
Endoplasmic Reticulum 0 0 1 1 0 1 1 1 1 1 0 1 2 1 1 4 4 2 10 0 1 2 1 2
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 3 0 2 2 0 6 2 3 0 1 1
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 1 0 0 1 0
Mitochondria 13 7 10 15 18 56 72 48 78 76 46 46 0 0 0 5 5 0 27 3 16 2 6 2
Nucleus 1 0 1 0 0 0 0 1 2 1 2 0 1 0 0 1 1 2 0 0 0 0 0 0
Nuclear Periphery 2 1 0 2 0 2 0 1 0 0 0 0 0 1 0 0 1 0 0 1 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 1 3 3 5 5 2 1 0 0 0 0 1 1 0 2 0 1 0 2 0
Vac/Vac Membrane 0 0 0 1 0 0 1 0 0 1 0 1 0 0 0 2 5 0 7 3 1 0 0 4
Unique Cell Count 72 267 218 173 277 247 232 259 252 254 115 136 282 260 314 84 96 47 233 269 195 184 311 180
Labelled Cell Count 77 273 234 192 300 289 286 297 306 297 144 165 285 264 321 95 107 53 233 269 195 184 311 180


Cytoplasm, Punctate Nuclear

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.9 4.0 3.2 3.1 3.2 2.8 2.4 3.4 2.8 2.6 2.8 2.7 4.5 4.6 4.5 4.9 5.7 5.1 3.9 3.8 5.0
Std Deviation (1e-4) 1.1 0.8 0.6 1.1 1.0 0.9 0.9 5.3 1.1 1.0 1.3 1.3 1.3 1.6 1.5 1.9 1.8 1.3 1.4 1.0 1.7
Intensity Change (Log2) – – – -0.04 -0.0 -0.16 -0.38 0.09 -0.17 -0.3 -0.17 -0.25 0.51 0.54 0.51 0.63 0.85 0.7 0.3 0.26 0.66


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.6238 2.2772 2.7396 1.9809 3.2487 2.5586 2.9525 1.1555 1.6268 0.5863 1.8661 1.1567 1.0664 2.277 1.4671 2.0321 2.3659 1.728
Actin 0.0308 0.0001 0.0121 0.0095 0.0161 0.0207 0.2201 0.0001 0.043 0 0.0002 0.0015 0.0264 0.0047 0.0058 0.0117 0 0.0369
Bud 0.0005 0 0.0069 0.0063 0.0003 0.0002 0.0011 0 0.0002 0 0 0 0.0116 0.0004 0.007 0.0003 0 0.0015
Bud Neck 0.0026 0 0.0006 0.0001 0.0002 0.0002 0.0036 0 0.0016 0 0 0.0006 0.0122 0.0001 0.0001 0.0006 0 0.0043
Bud Periphery 0.0009 0 0.0097 0.0114 0.0009 0.0004 0.0014 0 0.0002 0 0 0.0001 0.0294 0.0003 0.0141 0.0004 0 0.0064
Bud Site 0.0087 0 0.0305 0.0011 0.0004 0.0001 0.0197 0 0.0012 0 0.0002 0.0001 0.0066 0.0043 0.0015 0.0023 0 0.0006
Cell Periphery 0.0006 0.0001 0.0008 0.0002 0.0002 0 0.0006 0.0001 0.0001 0.0001 0 0 0.0007 0.0003 0.0002 0.0002 0 0.0005
Cytoplasm 0.0107 0.1338 0.0872 0.0834 0.0103 0.1772 0.0201 0.0666 0.0338 0.018 0.0103 0.058 0.0843 0.0774 0.1201 0.0358 0.0156 0.1016
Cytoplasmic Foci 0.0331 0.0015 0.0213 0.0217 0.0026 0.0015 0.0539 0.0012 0.0501 0.0006 0.0264 0.0124 0.0044 0.0064 0.0038 0.0067 0.0005 0.0097
Eisosomes 0.0028 0.0001 0.0004 0.0002 0.0006 0.0001 0.003 0.0001 0.0004 0.0001 0.0003 0.0001 0.0003 0.0006 0.0003 0.0002 0 0.0003
Endoplasmic Reticulum 0.0018 0.0016 0.0045 0.0019 0.0015 0.0006 0.0115 0.0006 0.0004 0.0002 0.0005 0.0008 0.0018 0.0029 0.0013 0.0013 0.0002 0.003
Endosome 0.0171 0.0005 0.0192 0.0019 0.0193 0.0003 0.1022 0.0002 0.006 0 0.0781 0.0083 0.0104 0.0187 0.0035 0.026 0 0.0051
Golgi 0.0061 0.0001 0.0118 0.0006 0.0248 0.0001 0.0319 0 0.0141 0 0.0149 0.0017 0.0158 0.0053 0.0008 0.0168 0 0.007
Lipid Particles 0.0547 0.0002 0.0083 0.0011 0.0421 0 0.0745 0.0001 0.0107 0.0001 0.0675 0.001 0.0157 0.0006 0.0051 0.0065 0 0.0026
Mitochondria 0.0045 0.0001 0.012 0.0018 0.1237 0.0001 0.0147 0.0001 0.0021 0.0001 0.0019 0.0003 0.0047 0.0034 0.0024 0.0135 0 0.0016
None 0.7729 0.861 0.7573 0.8482 0.7399 0.7981 0.3366 0.9303 0.7649 0.9804 0.7891 0.9063 0.7628 0.8677 0.8258 0.873 0.9833 0.8015
Nuclear Periphery 0.0018 0.0002 0.0018 0.0019 0.0061 0.0001 0.0104 0.0001 0.0001 0.0001 0.0004 0.0011 0.003 0.0022 0.0043 0.0008 0 0.0037
Nucleolus 0.0034 0 0.0025 0.0004 0.0003 0 0.0009 0 0.0001 0 0.0001 0.0001 0.0003 0.0001 0.0002 0.0002 0 0.0003
Nucleus 0.0012 0.0002 0.0018 0.001 0.0006 0.0001 0.0012 0.0001 0.0001 0.0001 0.0001 0.0007 0.0006 0.0006 0.0006 0.0004 0 0.0013
Peroxisomes 0.0335 0 0.0026 0.0018 0.0052 0.0001 0.0813 0 0.0693 0 0.0075 0.0008 0.0045 0.0005 0.0008 0.0011 0 0.0057
Punctate Nuclear 0.0102 0.0001 0.0032 0.0038 0.0009 0.0001 0.0044 0.0001 0.0014 0 0.0008 0.006 0.0014 0.0003 0.0012 0.0006 0 0.005
Vacuole 0.0017 0.0003 0.0031 0.0011 0.001 0.0001 0.0044 0.0001 0.0002 0.0001 0.001 0.0002 0.0022 0.002 0.0005 0.0012 0 0.0011
Vacuole Periphery 0.0006 0 0.0024 0.0004 0.0032 0 0.0026 0 0.0001 0 0.0006 0.0001 0.001 0.0009 0.0006 0.0007 0 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 6.1414 2.44 1.8106 4.1994 3.0966 4.092 2.6213 4.9954 5.3237 4.7888
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2396 1654 628 1754 2237 2167 1020 483 4633 3821 1648 2237

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 702.68 817.34 890.99 901.42 760.69 787.90 927.11 939.93 730.69 800.64 913.35 909.73
Standard Deviation 74.37 113.69 111.23 118.81 86.78 102.44 107.22 128.10 85.66 108.44 110.18 121.91
Intensity Change Log 2 — 0.218069 0.342541 0.359332 — 0.050704 0.285432 0.305245 — 0.133494 0.313137 0.331469

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000060 0.000218 0.000383 0.000868 0.000032 0.000126 0.000357 0.000715 0.000046 0.000166 0.000367 0.000835
Bud Neck 0.002614 0.004882 0.006423 0.023503 0.001333 0.005368 0.006401 0.013305 0.001996 0.005158 0.006410 0.021301
Bud Site 0.000848 0.001334 0.000437 0.008659 0.000136 0.001133 0.001116 0.005648 0.000504 0.001220 0.000858 0.008009
Cell Periphery 0.000105 0.000063 0.000036 0.000112 0.000081 0.000093 0.000067 0.000108 0.000093 0.000080 0.000055 0.000111
Cytoplasm 0.060032 0.009620 0.001182 0.022254 0.009198 0.010518 0.004491 0.043166 0.035487 0.010129 0.003230 0.026769
Cytoplasmic Foci 0.003922 0.001080 0.000025 0.001705 0.000502 0.001663 0.000282 0.010904 0.002271 0.001410 0.000184 0.003691
Eisosomes 0.000010 0.000011 0.000017 0.000021 0.000007 0.000011 0.000020 0.000024 0.000009 0.000011 0.000019 0.000022
Endoplasmic Reticulum 0.000987 0.003929 0.005026 0.009458 0.001604 0.003973 0.005153 0.005268 0.001285 0.003954 0.005105 0.008553
Endosome 0.000870 0.000873 0.000130 0.002651 0.000206 0.000771 0.000382 0.007884 0.000550 0.000815 0.000286 0.003781
Golgi 0.000698 0.000454 0.000029 0.000971 0.000034 0.000331 0.000312 0.003483 0.000378 0.000384 0.000204 0.001514
Lipid Particles 0.000580 0.000399 0.000076 0.000453 0.000238 0.000879 0.000159 0.000482 0.000415 0.000671 0.000127 0.000459
Mitochondria 0.003106 0.001893 0.000535 0.005877 0.000277 0.003130 0.001654 0.002981 0.001740 0.002595 0.001228 0.005251
Mitotic Spindle 0.001559 0.001939 0.000085 0.020115 0.000342 0.002643 0.001505 0.046268 0.000972 0.002338 0.000964 0.025762
None 0.009472 0.003861 0.000803 0.003530 0.003781 0.002831 0.002461 0.012793 0.006724 0.003277 0.001829 0.005530
Nuclear Periphery 0.000665 0.001727 0.000388 0.003315 0.000875 0.002061 0.000638 0.001133 0.000766 0.001916 0.000543 0.002844
Nuclear Periphery Foci 0.000388 0.000650 0.000082 0.000734 0.000129 0.001081 0.000090 0.000593 0.000263 0.000894 0.000087 0.000703
Nucleolus 0.002635 0.004442 0.003224 0.001495 0.003428 0.004349 0.002373 0.001813 0.003018 0.004389 0.002697 0.001563
Nucleus 0.908232* 0.959992* 0.980176* 0.879887* 0.977172* 0.955737* 0.969793* 0.822958* 0.941519* 0.957579* 0.973750* 0.867595*
Peroxisomes 0.000631 0.000573 0.000088 0.000445 0.000090 0.000614 0.000072 0.000456 0.000370 0.000596 0.000078 0.000448
Vacuole 0.002279 0.001585 0.000793 0.012203 0.000469 0.002212 0.002419 0.015743 0.001405 0.001941 0.001799 0.012967
Vacuole Periphery 0.000308 0.000474 0.000063 0.001745 0.000065 0.000478 0.000254 0.004274 0.000190 0.000476 0.000181 0.002291

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.94 -11.57 -9.21 -6.71 -4.76 -6.29 -18.42 -10.75 -9.67 -6.31 -5.95 -20.32 -12.24 -10.18 -6.42
Bud Neck -3.47 -6.53 -14.30 -12.38 -10.82 -5.61 -7.93 -6.93 -4.98 -3.97 -6.30 -9.71 -16.29 -13.29 -11.37
Bud Site -1.12 1.56 -6.37 -5.31 -6.90 -3.73 -3.00 -5.76 -4.71 -4.44 -2.79 -1.88 -8.22 -6.93 -7.26
Cell Periphery 3.46 6.28 0.44 -4.36 -9.46 -0.71 1.17 -1.96 -0.70 -3.18 1.24 4.65 -1.00 -2.18 -7.00
Cytoplasm 17.30 21.46 12.76 -6.40 -13.66 -1.02 3.19 -6.65 -6.34 -7.54 14.93 19.87 4.90 -8.93 -13.65
Cytoplasmic Foci 4.37 7.33 5.17 0.75 -2.82 -2.29 1.55 -2.19 -1.49 -2.35 1.94 7.26 1.28 -0.26 -3.21
Eisosomes -0.98 -6.37 -15.51 -12.19 -6.86 -4.34 -13.40 -12.33 -11.00 -6.39 -3.13 -13.99 -21.26 -17.83 -7.76
Endoplasmic Reticulum -9.41 -13.92 -16.14 -10.20 -8.18 -9.23 -16.40 -11.16 -7.12 -4.23 -13.31 -21.60 -18.54 -12.22 -8.77
Endosome -0.03 2.77 -1.62 -1.52 -4.17 -3.27 -1.53 -3.15 -2.64 -2.98 -1.30 1.62 -3.87 -3.12 -4.74
Golgi 0.67 2.36 0.72 0.00 -2.48 -1.89 -1.04 -2.84 -2.20 -2.29 -0.04 1.10 -1.38 -1.42 -2.44
Lipid Particles 1.12 5.02 1.51 -0.12 -6.35 -1.78 0.42 -4.81 0.64 -5.78 -1.20 4.24 -0.86 0.95 -6.84
Mitochondria 1.37 3.55 -1.48 -3.55 -7.83 -4.10 -1.75 -6.00 0.78 -1.12 -1.49 0.91 -4.18 -2.32 -4.62
Mitotic Spindle -0.42 2.51 -5.91 -5.57 -6.87 -2.55 -2.29 -5.12 -4.73 -4.55 -2.05 -1.36 -8.04 -7.21 -7.05
None 5.10 9.08 5.87 0.25 -8.50 1.45 2.09 -2.81 -3.60 -3.88 5.26 8.09 2.78 -2.80 -6.26
Nuclear Periphery -2.11 3.93 -12.01 -4.16 -12.78 -3.44 0.06 -7.24 -0.69 -7.58 -3.95 0.92 -12.80 -4.91 -13.09
Nuclear Periphery Foci -0.78 1.28 -1.25 -0.10 -3.74 -2.07 0.03 -4.05 0.74 -4.30 -2.08 1.29 -2.92 0.66 -5.17
Nucleolus -2.35 -1.16 2.48 3.55 2.33 -0.94 1.82 2.02 2.88 0.39 -2.33 0.48 3.45 4.89 2.67
Nucleus -10.38 -16.80 4.43 13.87 19.44 6.21 4.31 12.29 10.26 10.94 -5.11 -9.60 14.08 17.36 20.26
Peroxisomes 0.13 3.27 2.20 1.47 -2.55 -3.27 0.61 -3.03 1.73 -3.47 -1.51 3.35 1.09 2.44 -3.69
Vacuole 0.96 2.09 -10.39 -11.06 -11.48 -4.82 -7.88 -8.57 -8.03 -7.58 -1.57 -3.39 -13.67 -13.38 -12.90
Vacuole Periphery -0.93 2.63 -1.72 -1.09 -2.57 -2.68 -1.86 -1.85 -1.40 -1.68 -2.38 0.35 -2.74 -1.76 -2.81
Standard name
Human Ortholog
Description Putative type-1 protein phosphatase targeting subunit; tethers Glc7p type-1 protein phosphatase to Gsy2p glycogen synthase; PIG2 has a paralog, GIP2, that arose from the whole genome duplication
Localization
Cell Percentages nucleus (98%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Pig2

Pig2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pig2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available