Standard name
Human Ortholog
Description Pho85p cyclin of the Pho80p subfamily; forms a functional kinase complex with Pho85p which phosphorylates Mmr1p and is regulated by Pho81p; involved in glycogen metabolism, expression is cell-cycle regulated; PCL7 has a paralog, PCL6, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0 0
Cytoplasm 0.99 0.97 1.0 0.96 0.93 0.92 0.93 0.87 0.8 0.81 0.76 0.81 0.95 0.99 1.0 0.95 0.95 0.94 0.98 0.96 0.93 0.87 0.86 0.82
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0.08 0.08 0.18 0.16 0.38 0.24 0.34 0.27 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 1 0 2 0 0 1 0 1 2
Bud 0 1 0 0 4 1 4 2 1 7 10 5 0 0 0 0 0 0 0 0 3 4 5 12
Bud Neck 0 0 1 0 0 0 1 2 0 4 1 2 0 0 0 0 0 0 0 0 1 0 0 2
Bud Site 0 1 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 10 3 0 3 0 2 2 0 3 2 2 1 1 18 14 10 0 0 1 0 0 1
Cytoplasm 128 200 399 198 230 230 154 228 117 193 229 255 124 291 285 361 247 261 128 208 367 214 219 253
Endoplasmic Reticulum 1 1 0 0 2 0 1 0 0 0 2 0 0 0 0 14 12 10 0 0 0 4 4 2
Endosome 0 0 0 0 0 0 0 1 0 0 2 0 0 0 0 6 6 5 1 0 3 0 1 1
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 2 0 0 0 0 1 3
Mitochondria 1 6 0 7 19 21 30 41 56 57 103 86 3 2 0 14 4 3 0 4 2 2 4 7
Nucleus 1 1 0 3 5 1 3 2 1 2 1 1 0 0 0 0 2 1 0 0 0 3 2 1
Nuclear Periphery 0 0 0 0 0 0 0 1 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Nucleolus 0 0 0 2 0 0 1 1 0 1 2 1 4 2 0 0 0 0 0 0 0 7 3 9
Peroxisomes 0 0 0 0 0 0 0 1 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 2
SpindlePole 0 0 0 0 0 0 0 3 0 1 0 3 0 0 0 1 1 0 0 0 0 1 1 4
Vac/Vac Membrane 0 0 0 3 4 12 1 5 1 2 4 2 0 0 0 6 3 9 0 0 1 3 4 0
Unique Cell Count 129 207 400 206 246 250 166 261 146 237 303 316 130 293 285 379 259 278 132 217 394 246 256 311
Labelled Cell Count 131 211 411 216 264 268 195 289 180 267 357 359 134 296 286 422 290 303 132 217 394 246 256 311


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.3 4.4 6.2 5.7 5.0 4.9 3.7 4.9 4.1 4.2 4.0 4.1 8.0 6.7 5.4 6.6 8.4 8.2 9.6 9.0 11.4
Std Deviation (1e-4) 0.7 0.6 1.4 1.4 1.6 1.1 1.2 1.4 1.2 1.1 0.8 0.8 10.7 5.1 1.3 1.5 2.0 1.7 14.5 14.5 22.4
Intensity Change (Log2) – – – -0.12 -0.31 -0.36 -0.73 -0.34 -0.61 -0.58 -0.65 -0.59 0.37 0.1 -0.2 0.09 0.43 0.4 0.62 0.54 0.88


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.1428 4.2863 4.6478 4.1916 3.9015 4.6578 6.109 4.202 4.3361 3.3625 4.6543 4.4426 1.8866 1.456 1.7193 2.1786 1.3289 1.7098
Actin 0.055 0.0013 0.0408 0.001 0.0665 0.0125 0.1549 0.0022 0.0218 0.0199 0.0111 0.0079 0.0692 0.0002 0.0131 0.0003 0.0182 0.0071
Bud 0.0017 0.0041 0.0065 0.0008 0.0004 0.001 0.0021 0.0023 0.002 0.0017 0.0001 0.0003 0.0026 0.0002 0.0018 0.0001 0.0006 0.0005
Bud Neck 0.0013 0.0003 0.0056 0.0003 0.0005 0.0007 0.0494 0.0002 0.0013 0.0006 0.0004 0.0003 0.0034 0.0001 0.003 0.0001 0.0005 0.0037
Bud Periphery 0.0036 0.0041 0.0031 0.0029 0.0004 0.001 0.0018 0.0026 0.003 0.0017 0.0002 0.0004 0.0034 0.0001 0.0024 0.0001 0.0009 0.0008
Bud Site 0.0061 0.0028 0.0145 0.0003 0.0007 0.0004 0.0124 0.0353 0.0157 0.001 0.0004 0.0003 0.0049 0.0106 0.0061 0 0.0006 0.0003
Cell Periphery 0.0005 0.0002 0.0006 0.0002 0.0001 0.0001 0.0007 0.0005 0.0008 0.0001 0.0001 0.0001 0.0003 0.0001 0.0002 0.0001 0.0001 0.0001
Cytoplasm 0.3399 0.4457 0.4156 0.5556 0.4564 0.4909 0.3063 0.5203 0.4393 0.5598 0.692 0.4778 0.2325 0.4397 0.3411 0.4074 0.3816 0.4682
Cytoplasmic Foci 0.0165 0.0071 0.022 0.0078 0.0146 0.0175 0.0397 0.0107 0.011 0.0703 0.0066 0.0061 0.0187 0.0021 0.0065 0.0028 0.0139 0.0052
Eisosomes 0.0007 0 0.0006 0.0001 0.0004 0.0001 0.0009 0.0001 0.0003 0 0.0002 0.0001 0.0004 0 0.0002 0.0001 0.0002 0.0001
Endoplasmic Reticulum 0.0049 0.0011 0.0027 0.0027 0.0028 0.0017 0.0113 0.0033 0.0028 0.0035 0.0053 0.0017 0.0045 0.0013 0.0014 0.0019 0.0016 0.002
Endosome 0.0133 0.0021 0.0093 0.0177 0.0113 0.0027 0.0447 0.0023 0.0044 0.0094 0.019 0.0042 0.0154 0.0003 0.0087 0.001 0.0025 0.0031
Golgi 0.0051 0.0021 0.005 0.0244 0.0091 0.0017 0.0155 0.0004 0.0063 0.0033 0.0053 0.0062 0.0048 0 0.0012 0.0001 0.0016 0.0006
Lipid Particles 0.0102 0.0001 0.0095 0.0015 0.0069 0.0023 0.0242 0.0006 0.0041 0.0016 0.0071 0.0011 0.0071 0 0.0006 0.0002 0.0053 0.001
Mitochondria 0.0048 0.0052 0.0116 0.003 0.0075 0.0007 0.0061 0.0005 0.0121 0.0016 0.0021 0.0115 0.0025 0.0001 0.001 0.0002 0.0006 0.0008
None 0.503 0.5137 0.418 0.3742 0.386 0.4484 0.1787 0.41 0.46 0.2787 0.2204 0.4767 0.5519 0.5404 0.6027 0.584 0.5437 0.4924
Nuclear Periphery 0.0142 0.0014 0.0016 0.0013 0.0024 0.0009 0.0224 0.0027 0.001 0.006 0.0147 0.0003 0.0186 0.0003 0.001 0.0005 0.0005 0.004
Nucleolus 0.0005 0.0001 0.0006 0.0002 0.0004 0.0007 0.0173 0.0002 0.0002 0.0004 0.0006 0 0.0032 0.0001 0.0006 0 0.0001 0.0004
Nucleus 0.0036 0.0056 0.0099 0.0017 0.0024 0.0015 0.04 0.0015 0.0013 0.0029 0.004 0.0006 0.0099 0.0037 0.002 0.0005 0.0006 0.0059
Peroxisomes 0.0095 0.0008 0.0162 0.0005 0.0139 0.0101 0.0384 0.0007 0.0099 0.0242 0.0023 0.0036 0.0261 0 0.0029 0.0001 0.0241 0.0006
Punctate Nuclear 0.003 0.0018 0.0046 0.0006 0.0163 0.0045 0.0225 0.0023 0.0015 0.0104 0.006 0.0005 0.0173 0.0002 0.001 0.0002 0.0023 0.0026
Vacuole 0.0015 0.0004 0.0014 0.0025 0.0008 0.0004 0.0086 0.001 0.0009 0.0022 0.0013 0.0003 0.0022 0.0003 0.0019 0.0005 0.0004 0.0004
Vacuole Periphery 0.0012 0.0001 0.0003 0.0007 0.0003 0.0002 0.0021 0.0002 0.0002 0.0007 0.0008 0.0001 0.001 0 0.0004 0.0001 0.0001 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 13.5032 13.7955 8.5307 8.2609 10.838 8.9972 11.6238 12.5525 8.3951 13.3324
Translational Efficiency 0.8842 0.822 0.8283 0.8387 0.8461 1.3888 1.4834 0.9463 1.532 1.209

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Pho85p cyclin of the Pho80p subfamily; forms a functional kinase complex with Pho85p which phosphorylates Mmr1p and is regulated by Pho81p; involved in glycogen metabolism, expression is cell-cycle regulated; PCL7 has a paralog, PCL6, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (75%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Pcl7

Pcl7


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pcl7-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available