Standard name
Human Ortholog
Description Constitutively expressed DL-glycerol-3-phosphate phosphatase; also known as glycerol-1-phosphatase; involved in glycerol biosynthesis, induced in response to both anaerobic and osmotic stress; GPP1 has a paralog, GPP2, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.15 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.19 0.2 0 0 0 0 0 0
Cytoplasm 0.95 1.0 0.99 0.97 0.94 0.99 0.94 0.94 0.92 0.92 0.92 0.87 0.99 0.98 0.99 0.98 0.91 0.91 0.93 0.94 0.96 0.92 0.92 0.9
Endoplasmic Reticulum 0 0 0 0 0 0 0 0.05 0.08 0.06 0 0.11 0 0 0 0.07 0.07 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 1 0 0 0 5 2 1 1 0 0 0 3 0 1 2 1 0 1 0 0 2 3 2 9
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 26 0 2 0 2 5 4 9 5 7 13 12 6 11 8 11 50 37 6 0 0 0 1 1
Cytoplasm 165 218 271 142 116 290 272 323 289 320 286 275 146 284 248 323 247 172 180 215 237 128 155 225
Endoplasmic Reticulum 0 0 0 0 0 3 13 16 24 21 12 34 0 0 0 22 20 8 1 0 0 0 0 1
Endosome 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 2 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 1 0
Mitochondria 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 2 1 0 0 0 0 0 0 0 4 0 2 2 1
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 1 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 4 4 2 0 1 3 0 2 1 3 0 0 2 0 3 1 0 3 0 1 0 1
Unique Cell Count 173 218 275 146 123 294 289 345 315 347 310 317 147 289 250 328 270 189 193 228 248 140 169 249
Labelled Cell Count 192 218 277 149 125 300 291 352 319 351 316 329 152 296 261 357 320 219 193 228 248 140 169 249


Ambiguous, Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 498.3 304.2 333.9 282.8 170.9 192.3 150.8 137.1 119.2 112.0 104.5 94.6 412.4 393.8 378.1 400.1 485.8 490.0 384.7 421.0 384.1
Std Deviation (1e-4) 89.2 75.8 77.4 79.3 71.8 46.8 43.8 38.5 34.4 35.7 29.3 28.3 106.2 103.5 108.7 101.1 118.5 105.6 138.0 126.1 136.8
Intensity Change (Log2) – – – -0.24 -0.97 -0.8 -1.15 -1.28 -1.49 -1.58 -1.68 -1.82 0.3 0.24 0.18 0.26 0.54 0.55 0.2 0.33 0.2


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 2.6 2.4 0 2.4 0 2.2 7.1 7.2
Cytoplasm -0.9 -2.4 0.1 -2.8 -3.0 -3.8 -3.6 -3.6 -5.3 0.7 -0.3 0.7 -0.1 -3.8 -3.8
Endoplasmic Reticulum 0 0 0 3.6 3.6 4.7 4.2 3.3 5.6 0 0 0 4.4 4.6 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 270.5764 407.6605 322.9772 304.7513 278.351 410.4322 375.8885 495.3962 409.9495 370.6412 350.953 464.9706 355.9624 429.5762 405.3424 342.1148 374.3005 383.4141
Actin 0.0303 0.0003 0.0068 0.0028 0.0005 0.0003 0.0006 0.0001 0.0011 0.0002 0.0002 0.0001 0.0026 0.0005 0.0003 0.0002 0.0005 0.0017
Bud 0.0037 0.0005 0.0013 0.0002 0.0109 0.0002 0.0008 0.0001 0.001 0.0001 0.0012 0.0001 0.0003 0.0002 0.0004 0.0022 0.0002 0.0001
Bud Neck 0.0062 0.0001 0.0005 0.0004 0.0004 0.0003 0.0002 0.0001 0.0004 0.0001 0.0002 0.0003 0.0002 0.0001 0.0001 0.0002 0.0002 0.0001
Bud Periphery 0.0023 0.0001 0.0004 0.0003 0.0032 0 0.0003 0 0.0006 0 0.0003 0 0.0001 0.0001 0.0001 0.0006 0.0001 0.0001
Bud Site 0.0025 0.0003 0.0009 0.0004 0.0003 0 0.0002 0 0.0013 0 0.0003 0 0.0002 0 0.0002 0.0001 0.0002 0.0001
Cell Periphery 0.0015 0.0003 0.0004 0.0002 0.0002 0.0001 0.0005 0 0.0012 0.0001 0.0008 0 0.0005 0 0.0003 0.0001 0.0015 0.0001
Cytoplasm 0.7711 0.9845 0.9076 0.8407 0.9419 0.9628 0.9272 0.994 0.9166 0.9677 0.8244 0.9836 0.9227 0.994 0.9495 0.9597 0.8159 0.9024
Cytoplasmic Foci 0.0231 0.0004 0.0045 0.0305 0.0004 0.0003 0.0089 0.0001 0.0077 0.0066 0.03 0.0001 0.0025 0.0001 0.0017 0.0001 0.0014 0.0001
Eisosomes 0.0013 0 0.0006 0.0007 0 0 0.0002 0 0.0007 0 0.0007 0 0.0005 0 0.0001 0 0.0006 0.0005
Endoplasmic Reticulum 0.0009 0 0.0004 0.0011 0.0002 0.0001 0.0004 0 0.0007 0.0006 0.0001 0 0.0117 0.0001 0.0001 0.0001 0.0004 0
Endosome 0.0041 0 0.005 0.0296 0 0 0.0011 0 0.0012 0.0035 0.0002 0 0.0023 0 0.0001 0.0003 0.0001 0
Golgi 0.0038 0 0.0015 0.0158 0 0 0.0003 0 0.0003 0.0002 0.0001 0 0.0043 0 0 0 0 0.0001
Lipid Particles 0.0107 0 0.0068 0.0094 0 0 0.0051 0 0.0014 0.0001 0.0049 0 0.0016 0 0.0003 0 0.0006 0.0002
Mitochondria 0.0283 0.0014 0.0213 0.0194 0.0064 0.0005 0.0059 0.0001 0.0162 0.0004 0.0249 0.0001 0.0046 0.0001 0.0032 0.0008 0.0035 0.0874
None 0.0093 0.0011 0.009 0.0099 0.008 0.0006 0.0114 0.0004 0.0148 0.002 0.0449 0.0002 0.0041 0.0003 0.0041 0.0004 0.0208 0.0002
Nuclear Periphery 0.0109 0.0011 0.0019 0.0039 0.0029 0.0044 0.0032 0.0005 0.0028 0.0026 0.0028 0.0015 0.0092 0.0007 0.0031 0.0048 0.0396 0.0005
Nucleolus 0.0272 0.0004 0.0105 0.013 0.0007 0.0004 0.0073 0 0.013 0.0005 0.0375 0 0.008 0 0.0094 0.0002 0.0374 0.0001
Nucleus 0.0484 0.0089 0.0145 0.0144 0.0231 0.029 0.0206 0.0045 0.0133 0.0137 0.0163 0.0137 0.0215 0.0037 0.0232 0.0135 0.0671 0.0056
Peroxisomes 0.0072 0 0.0018 0.0018 0.0001 0 0.0008 0 0.0007 0.0002 0.0017 0 0.0002 0 0 0.0001 0.0001 0.0003
Punctate Nuclear 0.0024 0.0001 0.0021 0.0012 0.0002 0.0004 0.0017 0 0.001 0.0006 0.0016 0.0001 0.0005 0 0.0018 0.0001 0.004 0
Vacuole 0.0028 0.0002 0.0013 0.0019 0.0006 0.0003 0.0019 0.0001 0.0022 0.0006 0.0034 0.0001 0.001 0.0001 0.0008 0.0077 0.003 0.0001
Vacuole Periphery 0.002 0.0001 0.0009 0.0021 0.0002 0.0002 0.0015 0 0.0017 0.0003 0.0033 0 0.0014 0 0.0011 0.0087 0.0026 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 1358.3744 1536.5461 1598.9786 1423.8419 1385.0149 1562.6571 1886.7038 1702.0355 1841.2912 1411.5611
Translational Efficiency 1.4534 1.4892 1.4677 1.7344 1.553 1.6133 1.6326 1.6627 1.6134 1.8415

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Constitutively expressed DL-glycerol-3-phosphate phosphatase; also known as glycerol-1-phosphatase; involved in glycerol biosynthesis, induced in response to both anaerobic and osmotic stress; GPP1 has a paralog, GPP2, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-1

Gpp1

Gpp1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Gpp1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available