ORF
Human Ortholog
Description Putative protein of unknown function
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 – – 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0.07 0 0 0 – 0 0 – – 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 – – 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 – – 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 – – 0 0 0 0 0 0 0
Cytoplasm 0.98 0.98 1.0 0.95 0.97 0.94 0.93 0.87 0.69 0.8 0.57 0.67 – 1.0 0.98 – – 0.85 0.85 0.86 0.65 0.94 0.86 0.92
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 – – 0.12 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 – – 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 – – 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.13 0 0 0.11 0.08 0.54 0.24 0.62 0.45 – 0 0 – – 0.05 0.09 0.07 0.23 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 – – 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 – – 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 – – 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 – – 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 – – 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 – – 0.05 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 1 1 1 0 3 1 2 – 0 0 – – 0 4 0 0 0 2 1
Bud 1 1 0 0 3 0 0 4 4 2 4 4 – 0 0 – – 0 1 1 0 0 2 1
Bud Neck 2 1 0 0 0 1 2 5 0 1 0 2 – 0 0 – – 0 1 1 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 – – 0 – – – – – –
Cell Periphery 6 1 0 0 0 0 1 2 1 2 1 1 – 5 3 – – 1 0 1 0 0 0 0
Cytoplasm 330 221 20 58 138 145 208 201 41 150 97 125 – 140 135 – – 35 284 199 33 60 118 130
Endoplasmic Reticulum 1 3 0 0 0 4 1 2 0 2 2 3 – 0 3 – – 5 1 1 0 0 0 0
Endosome 0 0 0 0 0 1 3 2 0 1 0 0 – 0 0 – – 1 0 1 0 0 0 1
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 – – 0 1 1 0 0 0 0
Mitochondria 11 2 0 8 4 6 24 18 32 44 106 85 – 0 0 – – 2 30 15 11 0 3 3
Nucleus 0 0 0 1 2 3 0 2 0 0 1 1 – 0 0 – – 1 0 0 0 0 0 0
Nuclear Periphery 0 1 0 0 0 0 0 0 0 0 1 0 – 0 0 – – 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 – – 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 1 0 0 0 0 – 0 0 – – 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 2 0 0 4 0 2 1 3 – 0 0 – – 0 2 1 0 0 1 0
Vac/Vac Membrane 0 0 0 1 1 0 1 2 2 4 2 1 – 0 0 – – 2 3 2 1 0 1 0
Unique Cell Count 336 225 20 61 142 154 224 230 59 187 170 187 140 138 41 337 233 51 65 137 142
Labelled Cell Count 351 230 20 68 151 161 241 244 80 211 216 227 145 141 48 337 233 51 65 137 142


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.7 4.6 4.5 4.6 6.1 4.6 4.9 5.1 4.0 4.5 4.0 4.1 – 5.7 6.0 – – 9.1 – 5.1 5.8
Std Deviation (1e-4) 0.4 0.9 0.6 1.0 3.8 1.0 1.7 1.6 1.0 1.1 1.3 1.3 – 1.1 1.3 – – 2.2 – 1.4 1.5
Intensity Change (Log2) – – – 0.02 0.44 0.04 0.11 0.16 -0.18 -0.02 -0.18 -0.14 – 0.33 0.4 – – 1.01 – 0.18 0.35


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.3124 -0.2559 -0.2614 -0.627 -0.0813 -0.275 -0.5111 -0.044 -0.3212 -0.5542 -0.5399 -0.2541 -1.1949 -1.2364 -1.0464 -1.06 -0.1636 -1.22
Actin 0.0458 0.0061 0.0086 0.0108 0.0393 0.0118 0.0349 0.0025 0.0323 0.0034 0.0001 0.0007 0.016 0.0006 0.0092 0.0069 0.0193 0.0054
Bud 0.0031 0.0001 0.0005 0.0162 0.0002 0.0005 0.0013 0.004 0.0015 0.0003 0 0.0002 0.0022 0.0007 0.0013 0.0007 0.0008 0.0003
Bud Neck 0.0138 0.0001 0.0092 0.0005 0.0001 0.013 0.0009 0.0006 0.0005 0.0004 0.0001 0.0008 0.0129 0.0001 0.0016 0.0111 0.0007 0.0005
Bud Periphery 0.0035 0.0002 0.0009 0.0097 0.0002 0.0013 0.0026 0.0044 0.0022 0.0003 0.0001 0.0002 0.0034 0.0009 0.0014 0.0012 0.0021 0.0008
Bud Site 0.007 0.0007 0.0036 0.0005 0.0012 0.0012 0.0053 0.0128 0.0079 0.0003 0 0.0003 0.0134 0.0071 0.0104 0.0077 0.0025 0.0001
Cell Periphery 0.0007 0.0002 0.0004 0.0003 0.0001 0.0002 0.0006 0.0005 0.0003 0.0001 0.0001 0.0001 0.0013 0.0003 0.0002 0.0002 0.0001 0.0001
Cytoplasm 0.0938 0.1635 0.1988 0.1954 0.1773 0.2311 0.1158 0.2422 0.1652 0.3657 0.3281 0.298 0.1256 0.2396 0.1595 0.1925 0.1705 0.3655
Cytoplasmic Foci 0.0545 0.0085 0.016 0.0071 0.0207 0.0133 0.029 0.0125 0.0196 0.03 0.0042 0.0229 0.0167 0.0106 0.0193 0.0216 0.0693 0.0129
Eisosomes 0.0007 0.0004 0.0004 0.0003 0.0002 0.0003 0.0005 0.0002 0.0004 0.0002 0 0.0001 0.0003 0.0001 0.0002 0.0001 0.0003 0.0002
Endoplasmic Reticulum 0.0056 0.0021 0.0065 0.0011 0.0124 0.0027 0.0051 0.0024 0.0023 0.0027 0.002 0.0021 0.0026 0.0022 0.0021 0.0085 0.0016 0.0018
Endosome 0.0242 0.0023 0.0115 0.006 0.0134 0.0103 0.0113 0.008 0.0056 0.0353 0.0017 0.0062 0.026 0.0038 0.0103 0.0104 0.0197 0.0117
Golgi 0.0126 0.0005 0.0041 0.0065 0.0034 0.0049 0.0031 0.0007 0.0019 0.0007 0.0001 0.0006 0.0045 0.0004 0.0045 0.0036 0.0124 0.0023
Lipid Particles 0.0146 0.0011 0.0037 0.005 0.0047 0.0033 0.0118 0.0007 0.0026 0.0007 0.0001 0.0008 0.0077 0.0009 0.0058 0.0023 0.012 0.001
Mitochondria 0.0113 0.0004 0.0145 0.0219 0.0008 0.0182 0.0054 0.0007 0.0016 0.0006 0.0002 0.0006 0.0023 0.0003 0.0029 0.0008 0.0493 0.0048
None 0.6335 0.8102 0.694 0.7133 0.691 0.677 0.7456 0.6979 0.7414 0.5454 0.6617 0.6516 0.7338 0.7289 0.7581 0.7207 0.5204 0.5884
Nuclear Periphery 0.0081 0.0005 0.0049 0.0007 0.0017 0.0022 0.0044 0.0013 0.002 0.001 0.0003 0.0012 0.0028 0.0005 0.0011 0.0021 0.0356 0.0006
Nucleolus 0.0012 0.0002 0.0008 0.0002 0.0001 0.001 0.0008 0.0009 0.0004 0.0003 0 0.0005 0.0008 0.0001 0.0005 0.0002 0.0007 0.0001
Nucleus 0.0056 0.0005 0.0019 0.0008 0.0005 0.0016 0.0017 0.002 0.0016 0.0012 0.0003 0.0022 0.0018 0.0007 0.0009 0.001 0.0149 0.0006
Peroxisomes 0.0218 0.0009 0.0121 0.0012 0.026 0.001 0.0094 0.001 0.0038 0.0007 0 0.0055 0.0137 0.0002 0.0059 0.0042 0.0469 0.0005
Punctate Nuclear 0.0343 0.0005 0.0017 0.0005 0.0054 0.0017 0.0081 0.0026 0.0037 0.0022 0.0001 0.0039 0.004 0.0006 0.0026 0.0008 0.0173 0.0005
Vacuole 0.0028 0.001 0.0029 0.0013 0.0011 0.0025 0.0018 0.0018 0.0026 0.0069 0.0009 0.0013 0.007 0.0012 0.0018 0.003 0.0014 0.0014
Vacuole Periphery 0.0013 0.0001 0.0031 0.0006 0.0002 0.0008 0.0008 0.0003 0.0005 0.0013 0.0001 0.0003 0.0013 0.0002 0.0004 0.0007 0.0021 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 2.3427 2.6178 5.0505 5.8142 1.139 7.0242 5.6907 10.3613 9.8845 6.1029
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
206 1417 302 1422 1247 1129 1619 1650 1453 2546 1921 3072

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 594.21 676.43 938.47 830.96 692.67 688.72 863.02 834.47 678.71 681.88 874.88 832.85
Standard Deviation 66.97 122.54 103.85 120.79 77.35 92.08 101.22 105.83 83.37 110.25 105.29 113.02
Intensity Change Log 2 — 0.186968 0.659338 0.483806 — -0.008251 0.317226 0.268692 — 0.085182 0.485313 0.372017

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000108 0.000241 0.000457 0.000686 0.000084 0.000617 0.000085 0.001023 0.000088 0.000408 0.000144 0.000867
Bud Neck 0.026411 0.028452 0.003294 0.007225 0.012062 0.045814 0.002383 0.006079 0.014096 0.036151 0.002526 0.006609
Bud Site 0.007770 0.014465 0.004330 0.048527 0.003021 0.026467 0.004431 0.035219 0.003694 0.019787 0.004415 0.041379
Cell Periphery 0.000439 0.000106 0.000237 0.000134 0.000127 0.000145 0.000095 0.000127 0.000171 0.000123 0.000118 0.000130
Cytoplasm 0.465800* 0.456234* 0.288981* 0.573631* 0.520477* 0.364053* 0.609686* 0.559155* 0.512725* 0.415357* 0.559268* 0.565856*
Cytoplasmic Foci 0.304077* 0.242080* 0.028371 0.032124 0.250096* 0.313040* 0.019815 0.028885 0.257749* 0.273546* 0.021160 0.030384
Eisosomes 0.000402 0.000107 0.000108 0.000043 0.000160 0.000229 0.000029 0.000045 0.000195 0.000161 0.000041 0.000044
Endoplasmic Reticulum 0.001182 0.000715 0.005862 0.001200 0.000815 0.000383 0.001120 0.001013 0.000867 0.000568 0.001866 0.001100
Endosome 0.010878 0.034369 0.007689 0.006484 0.013858 0.039193 0.002063 0.004633 0.013436 0.036509 0.002948 0.005490
Golgi 0.004861 0.005759 0.000135 0.003059 0.002833 0.012986 0.001357 0.001387 0.003121 0.008964 0.001165 0.002161
Lipid Particles 0.014810 0.008838 0.005039 0.001053 0.008065 0.007238 0.000906 0.000991 0.009021 0.008128 0.001556 0.001020
Mitochondria 0.010824 0.009011 0.000775 0.001805 0.002293 0.018275 0.000265 0.001881 0.003502 0.013119 0.000345 0.001846
Mitotic Spindle 0.000139 0.003447 0.001641 0.022417 0.000803 0.007500 0.006132 0.027901 0.000708 0.005244 0.005426 0.025363
None 0.005050 0.007118 0.003884 0.003040 0.003083 0.001606 0.003564 0.002755 0.003362 0.004674 0.003614 0.002887
Nuclear Periphery 0.000371 0.000600 0.002734 0.000852 0.000513 0.000240 0.001210 0.000985 0.000493 0.000440 0.001450 0.000923
Nuclear Periphery Foci 0.000355 0.000817 0.002988 0.001227 0.000364 0.000234 0.000831 0.000770 0.000363 0.000558 0.001170 0.000981
Nucleolus 0.001008 0.003528 0.001001 0.000230 0.000898 0.001788 0.000217 0.000277 0.000914 0.002756 0.000340 0.000255
Nucleus 0.105218 0.090913 0.494147* 0.155717* 0.126315 0.041998 0.278447* 0.190239* 0.123324 0.069222 0.312358* 0.174259*
Peroxisomes 0.003739 0.010047 0.003075 0.001382 0.003793 0.027542 0.000102 0.001874 0.003785 0.017805 0.000570 0.001646
Vacuole 0.033968 0.079896 0.144891 0.137067 0.049297 0.087029 0.067056 0.134021 0.047124 0.083059 0.079292 0.135431
Vacuole Periphery 0.002588 0.003258 0.000361 0.002099 0.001042 0.003627 0.000204 0.000740 0.001261 0.003421 0.000228 0.001369

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.91 -5.45 -2.19 -1.22 1.12 -7.07 -3.33 -2.69 -0.13 -2.47 -8.12 -6.22 -3.50 -0.96 -2.59
Bud Neck -0.38 4.93 4.60 11.49 -1.65 -11.39 8.66 6.59 14.62 -3.89 -11.19 9.85 7.80 18.58 -4.40
Bud Site -2.02 0.57 -4.17 -3.21 -5.50 -11.10 -1.71 -7.25 2.67 -6.12 -11.92 -1.16 -9.82 -0.76 -8.76
Cell Periphery 4.20 2.44 4.24 0.49 7.65 -1.19 3.53 3.76 3.58 -0.18 3.53 3.79 5.49 3.41 2.31
Cytoplasm 0.66 10.91 -0.11 -1.30 -15.58 13.96 0.44 5.81 -8.90 5.45 11.12 4.29 5.66 -6.55 0.85
Cytoplasmic Foci 3.91 18.74 18.80 37.76 -0.54 -6.65 37.88 37.12 41.00 -2.75 -2.11 41.97 41.17 55.20 -3.98
Eisosomes 5.12 4.76 6.10 11.71 9.17 -2.85 14.33 12.70 8.14 -5.72 2.31 12.74 12.85 10.87 -0.00
Endoplasmic Reticulum 1.95 -11.29 -1.95 -9.46 11.19 4.04 -7.96 -5.93 -14.67 2.90 3.04 -12.73 -6.87 -16.00 9.22
Endosome -9.36 1.68 4.45 15.13 5.50 -10.22 10.05 9.17 16.55 -1.64 -12.88 10.06 9.68 22.38 -0.28
Golgi -0.62 4.05 2.91 5.82 -2.09 -8.31 2.86 4.61 10.72 0.44 -7.87 3.88 4.71 12.21 -0.25
Lipid Particles 2.24 3.84 5.49 7.95 8.82 0.89 11.11 11.05 7.99 -0.66 1.01 11.24 12.14 11.02 5.29
Mitochondria 0.52 2.92 2.84 6.65 -0.79 -8.37 6.54 3.36 9.10 -2.56 -7.91 5.53 4.35 11.21 -3.13
Mitotic Spindle -4.66 -2.04 -7.29 -5.55 -5.32 -3.62 -4.72 -7.97 -4.06 -4.23 -4.79 -4.98 -10.54 -6.97 -5.47
None -1.21 1.32 1.40 4.01 0.09 2.13 -0.57 -1.63 -4.92 -2.21 -1.72 0.04 -0.33 1.87 -0.86
Nuclear Periphery -2.27 -17.33 -13.91 -8.81 11.18 2.44 -11.67 -11.07 -21.39 0.74 0.49 -15.53 -12.05 -18.90 6.50
Nuclear Periphery Foci -2.38 -7.94 -4.32 -2.62 4.56 2.12 -7.17 -2.89 -3.40 -0.40 -2.18 -10.39 -5.24 -3.88 0.53
Nucleolus -4.88 0.38 2.38 7.57 6.95 -1.67 5.22 4.47 2.76 -2.80 -5.34 4.84 5.27 7.37 1.90
Nucleus 1.48 -20.77 -7.09 -14.01 18.42 18.78 -26.58 -15.77 -32.10 10.97 12.68 -32.15 -16.07 -31.20 20.14
Peroxisomes -7.34 1.33 6.55 11.41 0.87 -11.42 10.11 6.70 12.99 -2.51 -13.13 8.89 8.27 16.51 -1.15
Vacuole -9.40 -12.52 -28.01 -23.48 -4.43 -8.78 -13.40 -27.56 -18.76 -14.97 -12.22 -17.37 -39.16 -30.11 -18.47
Vacuole Periphery -0.77 3.16 2.28 5.74 -1.81 -5.25 7.79 4.94 6.76 -1.42 -7.01 7.62 3.57 9.28 -2.59
ORF
Human Ortholog
Description Putative protein of unknown function
Localization
Cell Percentages cytoplasm (41%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

YIL055C

YIL055C


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Yil055c-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available