Standard name | |
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Human Ortholog | |
Description | Protein of unknown function; involved in energy metabolism under respiratory conditions; expression induced under carbon limitation and repressed under high glucose; RGI2 has a paralog, RGI1, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 5 | 4 | 1 | 1 | 1 | 2 |
Bud | 0 | 1 | 0 | 2 | 2 | 1 | – | 6 | 7 | 9 | 10 | 9 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 13 | 0 | 1 | 0 | 3 |
Bud Neck | 7 | 1 | 5 | 0 | 3 | 1 | – | 5 | 0 | 1 | 2 | 3 | 1 | 0 | 0 | 2 | 0 | 1 | 2 | 3 | 0 | 1 | 0 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 1 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 8 | 0 | 5 | 2 | 1 | 4 | – | 0 | 0 | 4 | 3 | 3 | 0 | 2 | 0 | 5 | 2 | 5 | 4 | 2 | 2 | 0 | 1 | 0 |
Cytoplasm | 696 | 597 | 285 | 153 | 255 | 277 | – | 373 | 314 | 389 | 320 | 254 | 200 | 162 | 199 | 177 | 148 | 142 | 656 | 514 | 197 | 152 | 109 | 111 |
Endoplasmic Reticulum | 4 | 6 | 2 | 1 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 5 | 8 | 11 | 2 | 2 | 2 | 0 | 1 | 2 |
Endosome | 0 | 1 | 0 | 0 | 0 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 8 | 3 | 2 | 1 | 1 | 2 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 2 | 2 | 1 | 0 | 1 | 0 |
Mitochondria | 16 | 6 | 19 | 35 | 24 | 113 | – | 179 | 251 | 226 | 243 | 202 | 1 | 0 | 0 | 4 | 3 | 5 | 18 | 16 | 22 | 10 | 3 | 5 |
Nucleus | 1 | 2 | 0 | 0 | 0 | 0 | – | 1 | 1 | 2 | 1 | 3 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 1 | 0 | 1 | 0 | 0 | 0 | – | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 7 | 0 | 1 | 0 | 1 | 1 | – | 3 | 2 | 6 | 6 | 3 | 0 | 0 | 1 | 0 | 0 | 0 | 5 | 6 | 2 | 1 | 0 | 1 |
Vac/Vac Membrane | 0 | 4 | 1 | 0 | 0 | 3 | – | 2 | 5 | 3 | 3 | 0 | 0 | 0 | 2 | 1 | 1 | 1 | 3 | 10 | 1 | 4 | 6 | 4 |
Unique Cell Count | 720 | 611 | 293 | 159 | 263 | 311 | 462 | 445 | 504 | 444 | 372 | 204 | 162 | 202 | 181 | 151 | 151 | 732 | 598 | 249 | 179 | 133 | 141 | |
Labelled Cell Count | 741 | 618 | 319 | 193 | 286 | 401 | 570 | 583 | 643 | 589 | 478 | 205 | 164 | 202 | 197 | 163 | 168 | 732 | 598 | 249 | 179 | 133 | 141 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.7 | 4.6 | 2.7 | 3.0 | 3.4 | 2.7 | – | 3.0 | 2.5 | 2.7 | 2.7 | 2.5 | 4.6 | 4.8 | 4.7 | 5.5 | 6.0 | 5.9 | 4.6 | 5.4 | 5.1 |
Std Deviation (1e-4) | 0.5 | 1.4 | 0.6 | 1.3 | 1.1 | 1.1 | – | 1.3 | 1.1 | 0.7 | 1.2 | 0.9 | 1.0 | 1.4 | 1.6 | 1.0 | 1.3 | 1.9 | 1.5 | 4.1 | 1.9 |
Intensity Change (Log2) | – | – | – | 0.12 | 0.32 | -0.02 | – | 0.14 | -0.13 | -0.03 | -0.05 | -0.11 | 0.74 | 0.82 | 0.79 | 1.01 | 1.12 | 1.11 | 0.76 | 0.97 | 0.89 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | -0.8909 | 0.0553 | -0.406 | -0.3888 | -0.4915 | -0.5749 | -0.3366 | -0.9393 | -0.8163 | -0.4727 | -0.612 | -0.8518 | 0.406 | 0.6438 | 0.6766 | -0.0778 | 0.0914 | 0.6967 |
Actin | 0.013 | 0.0004 | 0.0014 | 0.0311 | 0.0001 | 0.0006 | 0.0567 | 0.001 | 0.0098 | 0.0765 | 0.0745 | 0.0021 | 0.007 | 0.0002 | 0.0002 | 0.0005 | 0.0054 | 0.0001 |
Bud | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0 | 0.0001 | 0.0128 | 0.0021 | 0.0053 | 0.0174 | 0.001 | 0.0003 | 0.0001 | 0.0015 | 0.0009 | 0.0003 | 0.0005 | 0 |
Bud Neck | 0.0034 | 0.0001 | 0.0024 | 0.0097 | 0 | 0.0002 | 0.0058 | 0.0006 | 0.0005 | 0.0009 | 0.0005 | 0.0004 | 0.0008 | 0.0005 | 0 | 0 | 0.0068 | 0.0001 |
Bud Periphery | 0.0003 | 0 | 0.0002 | 0.0002 | 0 | 0.0001 | 0.0098 | 0.0007 | 0.0103 | 0.0394 | 0.0014 | 0.0007 | 0.0001 | 0.0009 | 0.0008 | 0.0006 | 0.0007 | 0 |
Bud Site | 0.0017 | 0.0003 | 0.0028 | 0.0029 | 0 | 0 | 0.0159 | 0.0108 | 0.004 | 0.0098 | 0.003 | 0.0001 | 0.0003 | 0.0071 | 0.0001 | 0.0001 | 0.0039 | 0 |
Cell Periphery | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0 | 0 | 0.0011 | 0.0003 | 0.0007 | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0 |
Cytoplasm | 0.0617 | 0.2336 | 0.1813 | 0.2427 | 0.1354 | 0.2562 | 0.0494 | 0.0502 | 0.0439 | 0.0754 | 0.0242 | 0.0908 | 0.0496 | 0.0725 | 0.0637 | 0.0612 | 0.1039 | 0.106 |
Cytoplasmic Foci | 0.0142 | 0.0067 | 0.0058 | 0.019 | 0.0106 | 0.0112 | 0.0304 | 0.0049 | 0.0061 | 0.0081 | 0.0133 | 0.0252 | 0.0084 | 0.0021 | 0.0027 | 0.0055 | 0.0375 | 0.0019 |
Eisosomes | 0.0006 | 0.0001 | 0.0001 | 0.0007 | 0 | 0.0001 | 0.0007 | 0.0005 | 0.0003 | 0.0003 | 0.0004 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.0033 | 0.0014 | 0.0013 | 0.0012 | 0.0006 | 0.0011 | 0.006 | 0.0006 | 0.0052 | 0.0025 | 0.0013 | 0.0016 | 0.0028 | 0.0005 | 0.0004 | 0.0013 | 0.0013 | 0.0004 |
Endosome | 0.0141 | 0.0031 | 0.0028 | 0.0029 | 0.001 | 0.002 | 0.0127 | 0.0002 | 0.0053 | 0.0024 | 0.0155 | 0.008 | 0.0094 | 0.0004 | 0.0003 | 0.0009 | 0.003 | 0.0004 |
Golgi | 0.0027 | 0.0003 | 0.0007 | 0.0029 | 0.0001 | 0.0001 | 0.0065 | 0.0001 | 0.0014 | 0.0035 | 0.0156 | 0.0027 | 0.0012 | 0 | 0 | 0.0001 | 0.0016 | 0 |
Lipid Particles | 0.0049 | 0.0002 | 0.0009 | 0.0062 | 0.0006 | 0.0002 | 0.0081 | 0.0006 | 0.008 | 0.0016 | 0.0079 | 0.0041 | 0.0022 | 0.0001 | 0.0001 | 0.0003 | 0.0092 | 0 |
Mitochondria | 0.001 | 0.0002 | 0.0007 | 0.0011 | 0.0003 | 0.0002 | 0.0047 | 0.0017 | 0.0081 | 0.003 | 0.0046 | 0.0013 | 0.0004 | 0.0001 | 0.0005 | 0.0003 | 0.0009 | 0.0002 |
None | 0.8555 | 0.7509 | 0.7947 | 0.6571 | 0.8493 | 0.7254 | 0.7223 | 0.9157 | 0.8818 | 0.7053 | 0.8278 | 0.8509 | 0.9004 | 0.9111 | 0.9241 | 0.9243 | 0.7853 | 0.8893 |
Nuclear Periphery | 0.0067 | 0.0004 | 0.0009 | 0.0007 | 0.0003 | 0.0003 | 0.0165 | 0.0006 | 0.0023 | 0.0073 | 0.0012 | 0.0016 | 0.0037 | 0.0002 | 0.0004 | 0.001 | 0.0019 | 0.0002 |
Nucleolus | 0.0013 | 0.0001 | 0.0003 | 0.0007 | 0.0001 | 0.0001 | 0.0007 | 0.0008 | 0.0004 | 0.0019 | 0.0002 | 0.0002 | 0.0003 | 0.0002 | 0.0004 | 0.0001 | 0.002 | 0 |
Nucleus | 0.0026 | 0.0007 | 0.001 | 0.0008 | 0.0003 | 0.0005 | 0.0203 | 0.0043 | 0.0013 | 0.0213 | 0.0004 | 0.0007 | 0.0014 | 0.0011 | 0.0012 | 0.0012 | 0.0014 | 0.0006 |
Peroxisomes | 0.0022 | 0.0001 | 0.0003 | 0.0126 | 0.0002 | 0.0002 | 0.008 | 0.0011 | 0.0014 | 0.0154 | 0.005 | 0.0044 | 0.0037 | 0.0001 | 0.0002 | 0.0003 | 0.0155 | 0 |
Punctate Nuclear | 0.009 | 0.0004 | 0.0011 | 0.0064 | 0.0007 | 0.0008 | 0.0082 | 0.0028 | 0.0021 | 0.0034 | 0.0012 | 0.004 | 0.0049 | 0.0009 | 0.0036 | 0.0016 | 0.0183 | 0.0003 |
Vacuole | 0.0009 | 0.0009 | 0.0008 | 0.0005 | 0.0003 | 0.0004 | 0.0024 | 0.0004 | 0.0012 | 0.0034 | 0.0006 | 0.0006 | 0.0024 | 0.0003 | 0.0002 | 0.0003 | 0.0007 | 0.0002 |
Vacuole Periphery | 0.0005 | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.001 | 0.0001 | 0.0005 | 0.0009 | 0.0004 | 0.0003 | 0.0007 | 0 | 0.0001 | 0.0001 | 0.0003 | 0 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | – | – | – | – | – | – | – | – | – | – |
Translational Efficiency | – | – | – | – | – | – | – | – | – | – |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | – |
Translational Efficiency | – |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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