Standard name | |
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Human Ortholog | |
Description | Subunit of the ARP2/3 complex; ARP2/3 is required for the motility and integrity of cortical actin patches; has mRNA binding activity |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 23 | 17 | 29 | 32 | 26 | 27 | – | 55 | 46 | 53 | 54 | 67 | 54 | 54 | 53 | 10 | – | 12 | 74 | 67 | 92 | 68 | 81 | 100 |
Bud | 8 | 0 | 9 | 18 | 8 | 6 | – | 25 | 29 | 39 | 17 | 34 | 5 | 13 | 8 | 1 | – | 0 | 2 | 1 | 2 | 4 | 1 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 4 | 2 | 0 | 2 | – | 5 | 4 | 5 | 4 | 8 | 2 | 0 | 0 | 0 | – | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 3 | 5 | 6 | 2 | 1 | – | 2 | 2 | 2 | 1 | 6 | 8 | 7 | 12 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 3 | 1 | 1 | 3 | 1 | 7 | – | 25 | 17 | 9 | 9 | 6 | 7 | 6 | 4 | 4 | – | 1 | 1 | 0 | 0 | 0 | 1 | 0 |
Endoplasmic Reticulum | 5 | 6 | 3 | 4 | 1 | 1 | – | 3 | 2 | 2 | 4 | 2 | 5 | 11 | 10 | 1 | – | 3 | 0 | 0 | 0 | 1 | 0 | 0 |
Endosome | 0 | 0 | 0 | 3 | 0 | 0 | – | 0 | 0 | 5 | 0 | 0 | 0 | 1 | 1 | 0 | – | 0 | 1 | 0 | 2 | 1 | 2 | 1 |
Golgi | 7 | 11 | 1 | 4 | 7 | 8 | – | 20 | 9 | 12 | 4 | 6 | 35 | 22 | 39 | 13 | – | 12 | 9 | 3 | 2 | 1 | 4 | 8 |
Mitochondria | 35 | 16 | 65 | 81 | 29 | 82 | – | 156 | 120 | 127 | 135 | 214 | 57 | 54 | 35 | 4 | – | 0 | 0 | 3 | 2 | 0 | 3 | 3 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 1 | – | 4 | 1 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 1 | 0 | 1 | – | 1 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 3 | 1 | 4 | 6 | 1 | 10 | – | 20 | 8 | 14 | 15 | 11 | 0 | 0 | 0 | 3 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Peroxisomes | 0 | 0 | 2 | 4 | 3 | 9 | – | 24 | 10 | 15 | 16 | 29 | 0 | 5 | 9 | 1 | – | 0 | 0 | 0 | 2 | 2 | 5 | 3 |
SpindlePole | 0 | 0 | 0 | 1 | 0 | 0 | – | 6 | 4 | 5 | 1 | 3 | 0 | 1 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 1 | 0 | 2 | 1 | 0 | 2 | – | 2 | 2 | 0 | 1 | 1 | 0 | 0 | 0 | 5 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Unique Cell Count | 50 | 39 | 72 | 102 | 41 | 112 | 229 | 166 | 180 | 169 | 253 | 101 | 100 | 99 | 33 | 17 | 93 | 80 | 108 | 84 | 103 | 125 | ||
Labelled Cell Count | 85 | 55 | 125 | 166 | 78 | 157 | 348 | 256 | 289 | 262 | 388 | 174 | 174 | 172 | 43 | 28 | 93 | 80 | 108 | 84 | 103 | 125 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 27.1 | 38.0 | 19.8 | 24.7 | 26.4 | 22.3 | – | 21.0 | 20.3 | 21.2 | 19.1 | 20.1 | 28.5 | 27.4 | 30.3 | 35.9 | – | 38.2 | 28.2 | 31.3 | 30.1 |
Std Deviation (1e-4) | 9.4 | 12.2 | 6.8 | 8.9 | 9.3 | 8.3 | – | 9.5 | 8.7 | 8.0 | 7.6 | 7.5 | 8.0 | 9.5 | 10.4 | 19.3 | – | 15.0 | 12.2 | 12.0 | 12.2 |
Intensity Change (Log2) | – | – | – | 0.32 | 0.42 | 0.17 | – | 0.08 | 0.03 | 0.1 | -0.05 | 0.02 | 0.53 | 0.47 | 0.61 | 0.86 | – | 0.95 | 0.51 | 0.66 | 0.61 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -1.2 | 2.4 | -2.3 | – | -2.7 | -1.9 | -1.7 | -1.2 | -2.3 | 1.7 | 1.8 | 1.7 | -1.0 | – | 2.3 |
Bud | 0.9 | 1.0 | -1.7 | – | -0.4 | 1.0 | 1.7 | -0.6 | 0.2 | -1.8 | 0.1 | -1.0 | 0 | – | 0 |
Bud Neck | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 |
Bud Site | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 |
Cell Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0.2 | 0 | 1.1 | 0 | – | 0 |
Cytoplasm | 0 | 0 | 0 | – | 2.5 | 2.4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1.6 | 1.4 | 0 | – | 0 |
Endosome | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 |
Golgi | 0 | 0 | 0 | – | 2.1 | 0 | 0 | 0 | 0 | 5.3 | 3.9 | 5.8 | 0 | – | 0 |
Mitochondria | -1.9 | -2.7 | -2.8 | – | -3.7 | -3.1 | -3.3 | -2.0 | -1.2 | -4.8 | -5.1 | -7.2 | -7.8 | – | -7.5 |
Nucleus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 |
Nuclear Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 |
Nucleolus | 0 | 0 | 0.8 | – | 0.9 | 0 | 0.6 | 0.9 | 0 | 0 | 0 | 0 | 0 | – | 0 |
Peroxisomes | 0 | 0 | 0 | – | 2.0 | 0 | 0 | 1.8 | 2.2 | 0 | 0 | 0 | 0 | – | 0 |
SpindlePole | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 |
Vacuole | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 21.4694 | 22.8342 | 21.7384 | 21.5739 | 20.7676 | 22.3648 | 18.3606 | 21.301 | 20.5494 | 20.0677 | 21.9628 | 21.7546 | 22.658 | 24.6112 | 24.2522 | 27.6948 | 21.6515 | 27.0781 |
Actin | 0.8653 | 0.8993 | 0.8674 | 0.9645 | 0.8895 | 0.8142 | 0.782 | 0.9156 | 0.8872 | 0.9106 | 0.8572 | 0.9499 | 0.7247 | 0.9121 | 0.9086 | 0.9794 | 0.8501 | 0.9442 |
Bud | 0.0003 | 0.0005 | 0.0007 | 0.0003 | 0.0006 | 0.0007 | 0.0009 | 0.0005 | 0.0012 | 0.0003 | 0.0012 | 0 | 0.0011 | 0.0003 | 0.0004 | 0.0001 | 0.0005 | 0.0002 |
Bud Neck | 0.0032 | 0.0028 | 0.0063 | 0.0042 | 0.0104 | 0.0076 | 0.0024 | 0.0023 | 0.007 | 0.0014 | 0.0042 | 0.0023 | 0.0009 | 0.0041 | 0.0023 | 0.0047 | 0.0096 | 0.0045 |
Bud Periphery | 0.0004 | 0.0004 | 0.0008 | 0.0004 | 0.0013 | 0.0019 | 0.0015 | 0.0005 | 0.0023 | 0.0008 | 0.0029 | 0 | 0.0018 | 0.0002 | 0.0005 | 0.0001 | 0.0009 | 0.0004 |
Bud Site | 0.0019 | 0.0559 | 0.0146 | 0.0012 | 0.0011 | 0.0018 | 0.0077 | 0.0343 | 0.0132 | 0.0007 | 0.0041 | 0.0001 | 0.0065 | 0.0247 | 0.0061 | 0.0002 | 0.001 | 0.0004 |
Cell Periphery | 0.0003 | 0.0004 | 0.0005 | 0.0001 | 0.0006 | 0.0025 | 0.0006 | 0.0008 | 0.0009 | 0.0002 | 0.0019 | 0.0001 | 0.0003 | 0.0004 | 0.0002 | 0.0001 | 0.0002 | 0.0002 |
Cytoplasm | 0.0073 | 0 | 0.0047 | 0.0006 | 0.0016 | 0.0001 | 0.0027 | 0.0001 | 0.0057 | 0 | 0.0001 | 0 | 0.0027 | 0 | 0.0001 | 0 | 0.0006 | 0.0001 |
Cytoplasmic Foci | 0.0103 | 0.0006 | 0.0043 | 0.0067 | 0.0022 | 0.0019 | 0.0192 | 0.0032 | 0.0016 | 0.0003 | 0.0125 | 0 | 0.0327 | 0.0002 | 0.0029 | 0 | 0.0036 | 0.0003 |
Eisosomes | 0.0333 | 0.0345 | 0.0509 | 0.0037 | 0.0077 | 0.0877 | 0.0773 | 0.0332 | 0.0371 | 0.011 | 0.0612 | 0.0466 | 0.0374 | 0.0554 | 0.0377 | 0.0149 | 0.0024 | 0.0426 |
Endoplasmic Reticulum | 0.0044 | 0 | 0.0002 | 0.0007 | 0.0088 | 0.0002 | 0.0023 | 0 | 0.0015 | 0.0007 | 0 | 0 | 0.0083 | 0 | 0.0002 | 0 | 0.0039 | 0.0002 |
Endosome | 0.0078 | 0 | 0.0023 | 0.0016 | 0.0366 | 0.0043 | 0.0164 | 0.0002 | 0.0123 | 0.0025 | 0.0003 | 0 | 0.0201 | 0 | 0.0026 | 0 | 0.0371 | 0.0011 |
Golgi | 0.0056 | 0.0016 | 0.0016 | 0.0035 | 0.0136 | 0.0373 | 0.0144 | 0.0014 | 0.0111 | 0.0071 | 0.0031 | 0.0004 | 0.0171 | 0.0002 | 0.005 | 0.0003 | 0.0286 | 0.0029 |
Lipid Particles | 0.0129 | 0.0001 | 0.0005 | 0.0032 | 0.0039 | 0.0088 | 0.0165 | 0.0005 | 0.0006 | 0.0011 | 0.0143 | 0 | 0.0374 | 0.0001 | 0.0203 | 0.0001 | 0.0146 | 0.0004 |
Mitochondria | 0.0189 | 0.0014 | 0.0328 | 0.003 | 0.0055 | 0.0263 | 0.0191 | 0.0052 | 0.0153 | 0.0602 | 0.0107 | 0.0001 | 0.0505 | 0.0012 | 0.0064 | 0 | 0.0286 | 0.0015 |
None | 0.0037 | 0 | 0.0019 | 0.0019 | 0.0012 | 0.0001 | 0.0029 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0 | 0.0063 | 0 | 0.0003 | 0 | 0.0014 | 0.0001 |
Nuclear Periphery | 0.0151 | 0 | 0.0007 | 0.0003 | 0.0103 | 0.0005 | 0.011 | 0 | 0.0004 | 0.001 | 0 | 0 | 0.0133 | 0 | 0.0004 | 0 | 0.0085 | 0.0003 |
Nucleolus | 0.0003 | 0.0001 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0006 | 0.0001 | 0 | 0.0001 | 0.0001 | 0 | 0.0013 | 0 | 0 | 0 | 0.0002 | 0 |
Nucleus | 0.001 | 0 | 0.0014 | 0.0001 | 0.0007 | 0.0001 | 0.0012 | 0 | 0.0002 | 0.0002 | 0 | 0 | 0.0015 | 0 | 0 | 0 | 0.0006 | 0 |
Peroxisomes | 0.0032 | 0.0023 | 0.003 | 0.0036 | 0.0011 | 0.0025 | 0.0125 | 0.0019 | 0.0006 | 0.0006 | 0.0258 | 0.0003 | 0.0238 | 0.0009 | 0.0054 | 0.0001 | 0.0023 | 0.0005 |
Punctate Nuclear | 0.0029 | 0 | 0.0051 | 0.0004 | 0.0009 | 0.0004 | 0.0067 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0063 | 0 | 0.0002 | 0 | 0.0017 | 0.0001 |
Vacuole | 0.0005 | 0 | 0.0002 | 0.0001 | 0.0011 | 0.0004 | 0.0012 | 0.0001 | 0.0012 | 0.0002 | 0.0001 | 0 | 0.0023 | 0 | 0.0001 | 0 | 0.0011 | 0 |
Vacuole Periphery | 0.0014 | 0 | 0.0001 | 0.0001 | 0.0012 | 0.0006 | 0.0008 | 0 | 0.0004 | 0.0008 | 0.0001 | 0 | 0.0037 | 0 | 0.0003 | 0 | 0.0027 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 75.9332 | 86.9708 | 77.1285 | 105.4201 | 103.0656 | 92.4924 | 91.1533 | 114.3591 | 123.1672 | 102.0601 |
Translational Efficiency | 2.7413 | 2.4339 | 2.8807 | 2.0527 | 2.1607 | 2.9759 | 2.6189 | 2.1108 | 2.2904 | 2.208 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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