ORF
Human Ortholog
Description Uncharacterized protein of unknown function
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0.05 0 0.06 0 0 0 0 – – – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Cytoplasm 0.97 0.97 0.98 0.97 0.96 0.91 – 0.87 0.77 0.76 0.61 0.64 0.99 1.0 0.99 – – – 0.8 0.89 0.53 0.73 0.74 0.75
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.11 – 0.19 0.35 0.39 0.54 0.5 0 0 0 – – – 0.08 0 0.27 0.14 0.08 0.07
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0.05 0 0 0 – – – 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0.06 0.07
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 2 0 1 0 0 – 0 0 0 0 0 0 0 0 – – – 1 2 6 1 6 5
Bud 0 7 0 2 1 2 – 7 24 16 24 12 0 0 0 – – – 0 1 1 4 16 16
Bud Neck 0 6 0 0 2 1 – 6 7 3 1 3 7 4 6 – – – 0 1 3 3 7 5
Bud Site 0 1 0 0 0 0 – 2 0 1 2 0 0 0 0 – – – – – – – – –
Cell Periphery 0 2 2 1 2 1 – 1 0 4 0 1 1 2 1 – – – 0 1 1 2 4 6
Cytoplasm 190 419 202 201 326 306 – 546 402 465 264 308 332 368 437 – – – 161 330 116 252 441 451
Endoplasmic Reticulum 3 0 3 0 0 0 – 0 0 0 0 0 3 2 3 – – – 2 0 7 3 5 4
Endosome 1 0 0 1 7 3 – 8 5 3 3 2 0 0 0 – – – 2 2 7 4 5 5
Golgi 1 0 0 0 0 0 – 1 0 0 0 0 0 0 0 – – – 3 0 0 1 1 0
Mitochondria 3 16 7 2 5 37 – 117 183 237 234 237 0 0 0 – – – 16 12 59 47 50 40
Nucleus 0 1 0 0 0 0 – 2 1 1 1 2 1 3 1 – – – 0 0 0 1 0 1
Nuclear Periphery 0 0 1 0 0 0 – 1 0 1 0 1 0 0 0 – – – 0 0 0 0 1 1
Nucleolus 0 0 0 1 0 0 – 0 0 1 0 0 0 0 0 – – – 0 0 0 0 0 1
Peroxisomes 0 0 0 2 2 0 – 4 0 2 3 0 0 0 0 – – – 0 0 0 0 0 0
SpindlePole 0 1 0 1 2 5 – 8 15 22 17 23 0 0 0 – – – 1 3 1 3 5 9
Vac/Vac Membrane 0 0 4 0 4 7 – 3 9 7 3 10 0 0 1 – – – 8 2 6 11 38 41
Unique Cell Count 196 431 207 207 338 335 628 525 609 435 478 335 369 442 202 373 218 344 596 601
Labelled Cell Count 198 455 219 212 351 362 706 646 763 552 599 344 379 449 202 373 218 344 596 601


Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.8 3.1 3.7 4.0 3.8 3.2 – 3.1 2.8 2.6 2.7 2.7 4.8 5.0 5.1 3.5 4.2 4.6 5.0 5.1 5.3
Std Deviation (1e-4) 0.8 0.4 1.2 1.7 1.9 1.2 – 1.2 0.7 0.6 0.4 0.7 1.2 1.3 1.4 1.0 1.4 1.5 1.4 1.5 1.8
Intensity Change (Log2) – – – 0.13 0.06 -0.22 – -0.25 -0.39 -0.47 -0.46 -0.44 0.4 0.44 0.48 -0.05 0.21 0.32 0.46 0.48 0.53


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.8047 -0.4134 -0.5344 -0.797 -0.7364 -0.5299 0.5178 0.3134 0.1669 -0.1621 0.7577 0.0044 -0.2279 0.6788 0.2036 0.4872 0.038 0.4011
Actin 0.0208 0.0002 0.0004 0.0002 0.0001 0.0028 0.036 0.0008 0.001 0.0003 0.0201 0.0007 0.0001 0.0009 0.0002 0.0002 0.0003 0.0001
Bud 0.0003 0.0002 0.0002 0.0001 0 0.0001 0.0014 0.0018 0.0051 0.0001 0.0004 0.0002 0 0.0029 0.0004 0.0002 0.0022 0
Bud Neck 0.0009 0.0003 0.0003 0 0 0.0008 0.0087 0.0008 0.0003 0 0.0084 0.0003 0 0.0001 0.0003 0 0.0001 0.0004
Bud Periphery 0.0005 0.0003 0.0003 0.0003 0 0.0002 0.0019 0.0024 0.0087 0.0002 0.0005 0.0006 0 0.0053 0.0011 0.0003 0.001 0
Bud Site 0.0058 0.0062 0.0012 0.0001 0 0 0.0166 0.0302 0.0062 0 0.003 0.0001 0.0001 0.0055 0.0018 0 0.0002 0
Cell Periphery 0.0002 0.0002 0.0001 0 0 0 0.004 0.001 0.0004 0.0001 0.0001 0.0001 0.0001 0.0003 0.0001 0 0.0002 0
Cytoplasm 0.061 0.126 0.0863 0.0595 0.0876 0.1092 0.0632 0.0944 0.056 0.1009 0.0743 0.131 0.1169 0.1997 0.1566 0.1171 0.1704 0.2195
Cytoplasmic Foci 0.0265 0.0088 0.0232 0.0118 0.0113 0.0109 0.0343 0.0042 0.0071 0.0361 0.0318 0.0135 0.0105 0.0172 0.0126 0.0086 0.0111 0.0097
Eisosomes 0.0006 0.0001 0.0001 0.0001 0.0001 0.0002 0.0009 0.0002 0.0002 0.0002 0.0001 0.0001 0.0003 0.0001 0.0001 0.0001 0.0001 0
Endoplasmic Reticulum 0.0018 0.0009 0.0013 0.0003 0.0004 0.0006 0.0033 0.0007 0.0007 0.0009 0.0022 0.0016 0.0012 0.0025 0.0012 0.0009 0.0012 0.0008
Endosome 0.0067 0.0037 0.0128 0.0057 0.0034 0.0029 0.0121 0.0008 0.0029 0.0087 0.0176 0.0062 0.0038 0.0181 0.0052 0.0068 0.0046 0.0042
Golgi 0.0022 0.0001 0.0003 0.0001 0.0001 0.0003 0.0048 0.0001 0.0002 0.0004 0.0063 0.0009 0.0002 0.0012 0.0002 0.0002 0.0002 0.0001
Lipid Particles 0.0045 0.0004 0.0009 0.0001 0.0001 0.0005 0.0155 0.0003 0.0006 0.0014 0.0086 0.0023 0.001 0.0053 0.0006 0.0003 0.0003 0.0002
Mitochondria 0.0012 0.0003 0.0011 0.0005 0.0001 0.0003 0.0025 0.0002 0.0004 0.0011 0.0018 0.0003 0.0002 0.0011 0.0007 0.0005 0.0004 0.0002
None 0.8586 0.8483 0.8627 0.9175 0.8932 0.8663 0.7449 0.8587 0.9076 0.8368 0.7398 0.837 0.8623 0.7316 0.813 0.8608 0.8038 0.7619
Nuclear Periphery 0.0012 0.0005 0.0009 0.0002 0.0001 0.0004 0.005 0.0002 0.0003 0.0006 0.0329 0.0004 0.0004 0.0007 0.0005 0.0004 0.0005 0.0003
Nucleolus 0.0008 0.0001 0.0005 0.0001 0.0001 0.0001 0.001 0.0003 0.0002 0.0002 0.0003 0.0001 0.0002 0.0002 0.0002 0.0001 0.0001 0
Nucleus 0.0007 0.0008 0.0011 0.0005 0.0002 0.0004 0.0057 0.0006 0.0005 0.0005 0.0072 0.0003 0.0005 0.0009 0.0011 0.0005 0.0008 0.0006
Peroxisomes 0.0016 0.0002 0.0007 0.0003 0.0001 0.0004 0.0197 0.0001 0.0002 0.0053 0.0364 0.0026 0.0002 0.0004 0.0004 0.0003 0.0002 0
Punctate Nuclear 0.0019 0.0005 0.0024 0.0006 0.0003 0.0007 0.016 0.0006 0.0007 0.0006 0.0018 0.0007 0.0005 0.0007 0.0008 0.0006 0.0007 0.0004
Vacuole 0.002 0.0018 0.0025 0.0017 0.0026 0.0028 0.0021 0.0014 0.0007 0.0048 0.0041 0.0012 0.0013 0.0046 0.0024 0.0017 0.0014 0.0014
Vacuole Periphery 0.0003 0.0002 0.0006 0.0002 0.0002 0.0003 0.0004 0.0001 0.0001 0.0007 0.0021 0.0001 0.0001 0.0006 0.0003 0.0004 0.0002 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 8.6669 10.6531 6.8271 10.4144 6.3208 18.407 10.8937 12.3919 14.5756 12.1818
Translational Efficiency 0.8008 0.5439 0.8329 0.6974 0.7723 0.4074 0.6324 0.4596 0.4226 0.5224

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
182 1136 1839 1108 1998 1324 2217 1664 2180 2460 4056 2772

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 862.56 686.74 795.82 803.13 689.88 701.17 826.50 786.28 704.30 694.51 812.59 793.02
Standard Deviation 119.45 99.56 101.85 116.68 77.37 86.32 94.81 101.57 94.65 92.94 99.25 108.18
Intensity Change Log 2 — -0.328861 -0.116183 -0.102991 — 0.023419 0.260669 0.188698 — -0.161623 0.063521 0.033954

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.002977 0.000167 0.000128 0.002656 0.000210 0.000968 0.000066 0.001636 0.000441 0.000598 0.000094 0.002044
Bud Neck 0.006764 0.024697 0.004177 0.005334 0.018732 0.040080 0.002992 0.007506 0.017733 0.032976 0.003529 0.006638
Bud Site 0.019657 0.007511 0.009092 0.040326 0.006367 0.008793 0.003407 0.027072 0.007476 0.008201 0.005985 0.032370
Cell Periphery 0.001875 0.000137 0.000091 0.000263 0.000138 0.000166 0.000075 0.000273 0.000283 0.000153 0.000082 0.000269
Cytoplasm 0.288484* 0.449550* 0.426954* 0.454657* 0.467079* 0.373481* 0.467650* 0.503545* 0.452169* 0.408609* 0.449198* 0.484004*
Cytoplasmic Foci 0.081680 0.249493* 0.008693 0.031552 0.223268* 0.325089* 0.009097 0.032488 0.211447* 0.290180* 0.008914 0.032114
Eisosomes 0.000132 0.000142 0.000018 0.000063 0.000200 0.000329 0.000021 0.000113 0.000194 0.000243 0.000019 0.000093
Endoplasmic Reticulum 0.007599 0.000913 0.001707 0.002066 0.000858 0.000389 0.001231 0.001084 0.001420 0.000631 0.001447 0.001477
Endosome 0.027853 0.023566 0.000848 0.008791 0.013945 0.031941 0.001030 0.006519 0.015106 0.028074 0.000948 0.007427
Golgi 0.006442 0.003577 0.000044 0.010244 0.002197 0.007005 0.000036 0.002584 0.002551 0.005422 0.000040 0.005646
Lipid Particles 0.003575 0.010572 0.000548 0.002521 0.012088 0.011890 0.000628 0.001464 0.011378 0.011281 0.000592 0.001887
Mitochondria 0.002674 0.005154 0.000359 0.003823 0.005326 0.010194 0.000466 0.004379 0.005105 0.007866 0.000418 0.004157
Mitotic Spindle 0.022534 0.001185 0.001479 0.045579 0.003503 0.003268 0.008411 0.017297 0.005092 0.002306 0.005268 0.028602
None 0.001232 0.003293 0.004835 0.003989 0.002094 0.001122 0.004198 0.004323 0.002022 0.002124 0.004487 0.004190
Nuclear Periphery 0.000885 0.000689 0.001184 0.001418 0.000477 0.000309 0.001697 0.001060 0.000511 0.000485 0.001464 0.001203
Nuclear Periphery Foci 0.001477 0.000592 0.000581 0.001322 0.000279 0.000221 0.000425 0.000409 0.000379 0.000392 0.000496 0.000774
Nucleolus 0.000302 0.002334 0.000336 0.000471 0.000889 0.001192 0.000382 0.000690 0.000840 0.001720 0.000361 0.000602
Nucleus 0.176744* 0.128601 0.518599* 0.242184* 0.171772* 0.089323 0.463816* 0.286102* 0.172187* 0.107461 0.488654* 0.268548*
Peroxisomes 0.002398 0.009924 0.000102 0.001715 0.005738 0.019053 0.000095 0.001616 0.005460 0.014837 0.000098 0.001655
Vacuole 0.341202* 0.076018 0.020069 0.138011 0.063584 0.073552 0.034133 0.097656 0.086761 0.074691 0.027756 0.113787
Vacuole Periphery 0.003514 0.001885 0.000154 0.003014 0.001256 0.001635 0.000145 0.002182 0.001445 0.001750 0.000149 0.002514

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 1.39 1.39 0.28 -2.59 -2.60 -1.14 1.57 -1.40 -0.17 -1.58 -0.73 1.83 -1.97 -1.08 -2.51
Bud Neck -7.96 0.87 0.44 8.78 -0.63 -7.82 11.14 7.95 13.20 -5.11 -7.55 10.93 8.87 15.64 -4.19
Bud Site 1.97 1.59 -1.66 -4.57 -3.94 -1.72 2.81 -6.13 -5.00 -7.80 -1.05 1.18 -7.15 -6.85 -8.11
Cell Periphery 1.03 1.08 0.98 -1.35 -2.82 -1.74 6.99 -3.24 -1.74 -7.27 0.83 1.82 0.25 -2.31 -6.82
Cytoplasm -9.18 -7.03 -5.39 6.54 2.68 10.15 7.29 1.70 -7.93 -4.96 5.35 6.46 3.87 -1.57 -2.29
Cytoplasmic Foci -20.52 8.02 5.76 34.79 -8.63 -13.27 47.52 41.36 46.25 -12.99 -12.96 47.04 41.85 56.85 -15.57
Eisosomes -2.22 9.38 4.49 11.58 -16.01 -5.11 18.56 6.46 8.68 -4.62 -3.21 19.66 9.16 10.29 -6.90
Endoplasmic Reticulum 2.12 1.38 1.09 -8.94 -2.76 4.65 -8.95 -5.01 -10.52 3.61 2.88 -4.87 -4.35 -12.36 0.82
Endosome -1.68 4.07 2.97 9.98 -5.90 -8.75 14.27 8.55 14.00 -5.71 -8.96 14.87 9.44 17.13 -8.13
Golgi -0.16 1.71 -0.37 -0.38 -2.91 -4.64 7.41 1.00 4.72 -2.57 -4.69 7.27 -0.37 3.20 -3.83
Lipid Particles -7.34 4.99 1.49 8.47 -4.35 0.14 15.53 14.61 12.31 -7.22 0.03 15.83 14.14 14.82 -6.84
Mitochondria -3.81 1.55 -0.45 3.94 -2.91 -3.30 5.41 1.99 5.10 -2.91 -2.63 5.57 2.58 6.10 -3.81
Mitotic Spindle 1.97 1.92 -1.80 -6.69 -6.63 0.19 -2.98 -5.03 -4.99 -2.32 1.62 -1.02 -6.88 -8.38 -6.42
None -1.82 -3.79 -2.24 -0.28 1.92 2.25 -4.03 -2.95 -6.31 0.28 -0.18 -5.06 -3.40 -4.07 1.39
Nuclear Periphery 2.88 -1.41 -7.30 -8.85 -9.02 3.66 -26.93 -14.89 -16.18 5.74 0.78 -28.19 -21.63 -15.32 -0.57
Nuclear Periphery Foci 3.61 2.76 -0.64 -6.65 -5.43 1.54 -3.29 -5.93 -7.31 1.31 -0.46 -4.55 -9.14 -8.38 -2.06
Nucleolus -4.33 -0.86 -1.56 4.13 -0.91 -1.87 3.48 0.34 1.59 -1.69 -3.65 4.03 1.16 4.18 -1.85
Nucleus 3.80 -15.76 -2.96 -16.17 26.86 16.27 -46.11 -17.99 -29.90 22.68 14.25 -58.19 -18.72 -32.71 34.67
Peroxisomes -7.84 3.19 1.47 9.28 -3.14 -8.83 9.48 7.02 12.74 -3.23 -9.16 9.66 7.58 15.56 -4.31
Vacuole 13.48 15.55 5.58 -20.12 -26.70 -2.70 1.47 -16.32 -13.63 -16.91 3.99 12.03 -17.98 -23.47 -30.72
Vacuole Periphery -0.06 1.73 0.48 1.01 -2.21 -0.94 3.04 -0.19 0.60 -2.92 -1.16 3.33 -0.06 1.15 -3.66
ORF
Human Ortholog
Description Uncharacterized protein of unknown function
Localization
Cell Percentages —
Cell Cycle Regulation No
Subcompartmental Group N/A

YIL067C

YIL067C


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Yil067c-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available