Standard name
Human Ortholog
Description Essential 88kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; anchors the assembled complex to sites of secretion; interacts with SM-like protein and SNARE regulator Sec1p and may recruit it to sites of secretion; binds to SNARE complexes binteracting with Sec9p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.33 0.44 0.17 0.16 0.08 0.11 0.05 0.13 0.14 0.13 0.15 0.16 0.25 – – 0.24 0.24 0.19 0.33 0.31 0.35 0 0.1 0.09
Bud 0.14 0.23 0.15 0.22 0.22 0.22 0.15 0.17 0.2 0.19 0.19 0.16 0.19 – – 0.22 0.18 0.14 0.32 0.37 0.29 0.33 0.47 0.53
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0.1 0 0.2 0.17 0.2 0.27 0.25 0.12
Bud Site 0.23 0.21 0.15 0.18 0.11 0.11 0.08 0.07 0.06 0.11 0.09 0.09 0.19 – – 0.15 0.17 0.09 – – – – – –
Cell Periphery 0.06 0.07 0 0 0 0 0 0.06 0.05 0.06 0.08 0.08 0 – – 0.05 0 0 0 0 0 0 0 0
Cytoplasm 0.13 0.15 0.18 0.25 0.22 0.21 0.16 0.17 0.14 0.12 0.13 0.11 0.12 – – 0.28 0.25 0.29 0 0.05 0 0.1 0.06 0.12
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0.12 – – 0 0 0.05 0 0 0 0 0 0
Endosome 0.06 0 0.11 0 0.16 0.07 0.09 0.06 0 0 0 0 0.38 – – 0 0 0.08 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0 0 0
Mitochondria 0.09 0.24 0.1 0.12 0.07 0.27 0.35 0.37 0.52 0.5 0.52 0.54 0.06 – – 0.07 0.1 0.05 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0.05 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0 0 0
Nucleolus 0.07 0 0 0 0 0.05 0.08 0 0 0.06 0.05 0 0.06 – – 0 0 0 0 0 0 0 0 0
Peroxisomes 0.19 0.19 0.4 0.29 0.34 0.25 0.23 0.23 0.19 0.19 0.18 0.2 0.06 – – 0.15 0.14 0.22 0 0 0 0 0 0
SpindlePole 0.14 0 0.17 0.18 0.15 0.1 0.11 0.12 0 0.07 0.06 0.06 0.25 – – 0.21 0.26 0.26 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 53 41 41 34 21 32 24 64 48 42 47 58 4 – – 117 64 42 66 36 94 1 8 8
Bud 23 22 37 45 56 65 69 80 67 61 58 59 3 – – 105 48 30 65 43 77 13 38 51
Bud Neck 4 2 3 2 3 4 7 4 8 9 4 2 0 – – 7 26 6 41 19 53 11 19 11
Bud Site 38 20 36 37 28 33 37 35 20 35 29 33 3 – – 73 45 19 – – – – – –
Cell Periphery 10 7 10 9 6 13 20 31 16 19 26 31 0 – – 22 7 8 2 1 5 0 0 0
Cytoplasm 21 14 44 52 55 63 73 82 47 39 39 42 2 – – 133 68 63 2 5 7 4 4 11
Endoplasmic Reticulum 2 3 5 3 2 6 5 11 10 12 9 9 2 – – 14 10 12 0 0 1 1 0 0
Endosome 9 4 26 7 40 20 39 27 11 8 5 8 6 – – 14 11 17 6 2 4 0 2 2
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 5 0 3 1 0 1 0 0 0
Mitochondria 15 23 24 25 18 82 159 175 175 163 162 197 1 – – 34 27 10 3 1 3 1 0 2
Nucleus 7 1 7 7 9 5 14 13 3 5 9 4 0 – – 25 7 9 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 1 0 2 0 2 1 0 0 0 – – 0 0 0 0 0 0 1 0 0
Nucleolus 11 2 9 4 10 15 36 20 15 19 14 6 1 – – 10 3 3 0 0 0 0 0 0
Peroxisomes 31 18 95 61 87 75 105 108 64 64 56 75 1 – – 73 38 49 3 1 9 0 0 0
SpindlePole 23 3 40 37 39 31 51 57 12 23 17 21 4 – – 101 71 57 6 3 6 1 1 2
Vac/Vac Membrane 1 0 4 1 5 3 12 10 1 4 1 6 0 – – 9 2 1 1 0 2 0 1 1
Unique Cell Count 162 94 240 207 253 300 451 477 336 329 309 367 16 483 271 221 203 118 270 41 81 98
Labelled Cell Count 248 160 381 324 380 447 653 717 499 504 476 551 27 742 427 329 203 118 270 41 81 98


Ambiguous, Bud, Bud Neck, Cell Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.8 6.9 6.2 5.9 6.0 5.3 5.0 5.5 5.1 4.9 4.9 5.1 6.0 – – 6.6 6.7 7.9 – 6.9 7.8
Std Deviation (1e-4) 1.4 1.4 1.4 1.6 2.0 1.6 1.5 1.4 1.2 1.2 1.3 1.3 0.6 – – 1.3 1.3 1.8 – 1.4 2.0
Intensity Change (Log2) – – – -0.06 -0.04 -0.23 -0.29 -0.17 -0.28 -0.33 -0.32 -0.28 -0.03 – – 0.09 0.12 0.36 – 0.17 0.34


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -0.2 -2.9 -2.2 -5.0 -1.3 -0.9 -1.4 -0.6 -0.4 0 – – 2.2 1.8 0.5
Bud 1.7 1.9 1.8 0 0.5 1.4 1.0 1.0 0.2 0 – – 2.0 0.7 -0.6
Bud Neck 0 0 0 0 0 0 1.2 0 0 0 – – 0 4.1 0
Bud Site 0.8 -1.3 -1.4 -2.8 -3.2 -3.6 -1.6 -2.0 -2.3 0 – – 0 0.5 -2.1
Cell Periphery 0.1 -1.1 0.1 0.2 1.3 0.3 0.9 2.0 2.1 0 – – 0.2 -1.0 -0.3
Cytoplasm 1.7 0.9 0.8 -0.7 -0.4 -1.4 -2.2 -1.9 -2.4 0 – – 2.7 1.8 2.6
Endoplasmic Reticulum 0 0 0 0 0.2 0.7 1.1 0.6 0.3 0 – – 0.6 1.1 1.9
Endosome -3.0 1.6 -1.7 -0.9 -2.5 -3.6 -4.2 -4.6 -4.5 0 – – -4.4 -2.9 -1.2
Golgi 0 0 0 0 0 0 0 0 0 0 – – 0 0 0
Mitochondria 0.7 -1.1 5.0 7.2 7.5 10.5 9.9 10.4 10.9 0 – – -1.4 0 -2.2
Nucleus 0.3 0.4 -1.0 0.1 -0.1 0 -1.1 0 0 0 – – 1.4 -0.2 0.7
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 – – 0 0 0
Nucleolus -1.1 0.1 0.7 2.1 0.3 0.4 1.1 0.5 -1.6 0 – – -1.3 -2.0 -1.6
Peroxisomes -2.2 -1.2 -3.6 -4.5 -4.8 -5.4 -5.3 -5.6 -5.1 -2.7 – – -7.3 -6.6 -4.0
SpindlePole 0.3 -0.4 -2.2 -2.0 -1.7 -5.4 -3.6 -4.3 -4.4 0 – – 1.4 2.6 2.4
Vacuole 0 0 0 0.8 0 0 0 0 0 0 – – 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.7609 2.3374 2.7062 2.9589 2.8904 2.7987 5.0162 3.9045 4.1821 4.5074 4.4364 4.7228 4.4501 3.8151 4.0244 3.7171 4.0954 4.706
Actin 0.2299 0.0964 0.139 0.3221 0.3079 0.1673 0.2152 0.08 0.1304 0.2868 0.4327 0.1423 0.2469 0.1132 0.1405 0.316 0.3278 0.146
Bud 0.0749 0.0907 0.2722 0.124 0.0901 0.0099 0.0263 0.078 0.2293 0.0924 0.0551 0.0089 0.0507 0.0995 0.251 0.1006 0.0632 0.0097
Bud Neck 0.0964 0.0091 0.0108 0.0027 0.065 0.6389 0.1084 0.0162 0.0301 0.0327 0.0538 0.6822 0.0527 0.0067 0.0102 0.0124 0.0249 0.6936
Bud Periphery 0.1074 0.0756 0.394 0.4846 0.3552 0.0237 0.0479 0.0663 0.4152 0.3343 0.2649 0.0309 0.1063 0.0807 0.4076 0.4008 0.3452 0.0264
Bud Site 0.2582 0.5227 0.0767 0.0512 0.0652 0.0305 0.3548 0.6341 0.1101 0.1263 0.0419 0.0237 0.3731 0.5115 0.1453 0.1007 0.0912 0.0216
Cell Periphery 0.0119 0.0081 0.0037 0.0026 0.0034 0.0035 0.0157 0.0041 0.0042 0.0182 0.0052 0.0055 0.0317 0.0083 0.0066 0.0041 0.0073 0.0067
Cytoplasm 0.0714 0.1056 0.0259 0.0029 0.0191 0.0329 0.0442 0.0609 0.019 0.0233 0.0184 0.0102 0.0526 0.0831 0.0113 0.0244 0.0191 0.0258
Cytoplasmic Foci 0.0589 0.0274 0.0223 0.002 0.0396 0.0483 0.0648 0.0235 0.009 0.0146 0.028 0.0487 0.0323 0.0136 0.0046 0.0121 0.0236 0.0347
Eisosomes 0.0021 0.0006 0.0007 0.0006 0.0019 0.0005 0.0059 0.0003 0.0004 0.0016 0.001 0.0007 0.003 0.0008 0.0006 0.0003 0.0014 0.0005
Endoplasmic Reticulum 0.0039 0.0024 0.0013 0.0003 0.0005 0.0005 0.0035 0.0007 0.0028 0.0014 0.0024 0.0032 0.0014 0.0006 0.0005 0.0009 0.0004 0.0002
Endosome 0.0107 0.002 0.0039 0.0004 0.0013 0.0033 0.0161 0.0035 0.0026 0.0007 0.0202 0.002 0.0017 0.0022 0.0004 0.0019 0.0009 0.0013
Golgi 0.0092 0.0026 0.0051 0.0011 0.0027 0.0041 0.0123 0.0017 0.0008 0.0019 0.0089 0.003 0.0024 0.0011 0.0008 0.0035 0.0027 0.0025
Lipid Particles 0.0067 0.0012 0.0033 0.0001 0.003 0.0019 0.0124 0.001 0.0008 0.0082 0.0024 0.0016 0.0028 0.0009 0.0004 0.0004 0.0041 0.0009
Mitochondria 0.0132 0.001 0.0039 0.0021 0.0053 0.0006 0.0066 0.0036 0.0013 0.0018 0.0036 0.0028 0.0039 0.0012 0.002 0.0022 0.004 0.001
None 0.0197 0.0404 0.0171 0.0012 0.0233 0.0103 0.0152 0.0126 0.0236 0.0166 0.0148 0.0091 0.0118 0.0568 0.0078 0.0042 0.0339 0.0082
Nuclear Periphery 0.002 0.0006 0.0005 0.0002 0.0003 0.0001 0.0105 0.0004 0.0014 0.0004 0.0017 0.0024 0.0005 0.0004 0.0002 0.0005 0.0003 0
Nucleolus 0.0012 0.0021 0.0003 0.0001 0.0009 0.0013 0.0015 0.0013 0.0013 0.0003 0.0008 0.0007 0.0008 0.0042 0.0015 0.0005 0.0023 0.0003
Nucleus 0.001 0.0012 0.0007 0.0002 0.0006 0.0005 0.0069 0.0016 0.0009 0.0008 0.0017 0.0014 0.001 0.0034 0.001 0.0012 0.0012 0.0003
Peroxisomes 0.015 0.0033 0.0151 0.0008 0.0093 0.0085 0.0174 0.0043 0.0067 0.0327 0.03 0.0161 0.0179 0.0037 0.0048 0.0062 0.0428 0.0163
Punctate Nuclear 0.0024 0.0051 0.002 0.0003 0.004 0.012 0.0088 0.0033 0.009 0.0011 0.0053 0.0031 0.0014 0.0051 0.0013 0.0008 0.0023 0.0033
Vacuole 0.0034 0.0017 0.0013 0.0006 0.0012 0.0014 0.0045 0.0022 0.0009 0.0037 0.0059 0.001 0.0046 0.0027 0.0014 0.0058 0.0012 0.0006
Vacuole Periphery 0.0004 0.0002 0.0003 0.0002 0.0002 0.0001 0.0013 0.0004 0.0001 0.0003 0.0013 0.0003 0.0004 0.0002 0.0002 0.0004 0.0002 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 14.8307 11.83 18.7675 20.7651 15.6788 13.2262 21.5509 22.6881 22.1602 20.5489
Translational Efficiency 0.6471 1.0428 0.6366 0.6668 0.6673 0.8338 0.6804 0.525 0.5598 0.6495

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Essential 88kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; anchors the assembled complex to sites of secretion; interacts with SM-like protein and SNARE regulator Sec1p and may recruit it to sites of secretion; binds to SNARE complexes binteracting with Sec9p
Localization
Cell Percentages actin (34%), bud neck (12%), mixed (20%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Sec6

Sec6


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sec6-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available