Standard name | |
---|---|
Human Ortholog | |
Description | Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO) |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 2 | 4 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 7 | 3 | 0 | 4 | 3 | 8 | 1 | 2 | 1 | 5 | 10 | 13 |
Bud | 4 | 1 | 7 | 7 | 6 | 8 | 19 | 11 | 13 | 9 | 16 | 11 | 2 | 3 | 2 | 4 | 3 | 14 | 0 | 0 | 1 | 0 | 2 | 4 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 5 | 7 |
Bud Site | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 2 | 5 | 6 | 4 | 2 | 0 | 0 | 0 | 0 | 3 | 2 | – | – | – | – | – | – |
Cell Periphery | 3 | 4 | 5 | 3 | 2 | 4 | 0 | 1 | 1 | 0 | 1 | 3 | 2 | 5 | 7 | 19 | 9 | 24 | 0 | 0 | 0 | 0 | 0 | 1 |
Cytoplasm | 7 | 3 | 9 | 18 | 18 | 23 | 22 | 20 | 7 | 6 | 13 | 17 | 18 | 36 | 55 | 22 | 22 | 12 | 0 | 0 | 0 | 3 | 2 | 4 |
Endoplasmic Reticulum | 3 | 4 | 3 | 2 | 0 | 5 | 3 | 0 | 1 | 0 | 3 | 0 | 5 | 19 | 3 | 7 | 5 | 8 | 0 | 0 | 0 | 0 | 1 | 3 |
Endosome | 1 | 4 | 10 | 6 | 25 | 7 | 1 | 7 | 1 | 0 | 0 | 0 | 24 | 37 | 53 | 16 | 9 | 10 | 15 | 5 | 8 | 17 | 21 | 30 |
Golgi | 0 | 5 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 3 | 6 | 13 | 16 | 9 | 4 | 11 | 3 | 6 | 11 | 10 |
Mitochondria | 194 | 80 | 88 | 131 | 121 | 241 | 302 | 216 | 259 | 190 | 240 | 212 | 109 | 59 | 58 | 28 | 23 | 10 | 141 | 77 | 137 | 150 | 218 | 211 |
Nucleus | 79 | 2 | 16 | 27 | 23 | 29 | 20 | 31 | 22 | 14 | 33 | 22 | 6 | 3 | 1 | 6 | 3 | 4 | 75 | 2 | 8 | 2 | 1 | 2 |
Nuclear Periphery | 2 | 0 | 0 | 0 | 0 | 3 | 5 | 2 | 6 | 4 | 8 | 2 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 4 |
Nucleolus | 16 | 2 | 0 | 0 | 1 | 3 | 8 | 7 | 3 | 4 | 6 | 6 | 0 | 0 | 0 | 0 | 0 | 1 | 6 | 0 | 0 | 0 | 3 | 6 |
Peroxisomes | 0 | 1 | 8 | 12 | 12 | 4 | 2 | 4 | 0 | 0 | 0 | 0 | 10 | 15 | 36 | 15 | 11 | 9 | 2 | 1 | 6 | 18 | 14 | 12 |
SpindlePole | 3 | 0 | 16 | 17 | 10 | 11 | 15 | 19 | 11 | 4 | 3 | 10 | 11 | 7 | 5 | 13 | 9 | 8 | 0 | 1 | 2 | 4 | 8 | 5 |
Vac/Vac Membrane | 21 | 14 | 6 | 6 | 24 | 21 | 16 | 13 | 22 | 7 | 12 | 22 | 31 | 29 | 62 | 45 | 13 | 16 | 11 | 5 | 8 | 9 | 19 | 16 |
Unique Cell Count | 255 | 94 | 140 | 192 | 204 | 297 | 337 | 282 | 284 | 208 | 270 | 252 | 187 | 182 | 240 | 152 | 106 | 108 | 262 | 109 | 187 | 223 | 330 | 343 |
Labelled Cell Count | 334 | 122 | 172 | 231 | 243 | 362 | 416 | 334 | 351 | 244 | 340 | 308 | 230 | 220 | 288 | 193 | 129 | 135 | 262 | 109 | 187 | 223 | 330 | 343 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.4 | 6.2 | 5.4 | 5.4 | 5.7 | 4.8 | 4.2 | 5.1 | 4.2 | 4.3 | 4.2 | 4.6 | 5.7 | 6.1 | 6.2 | 8.0 | 7.1 | 6.6 | 5.7 | 5.9 | 6.6 |
Std Deviation (1e-4) | 0.8 | 1.0 | 1.1 | 1.8 | 1.7 | 1.4 | 1.3 | 1.4 | 1.1 | 0.9 | 1.5 | 1.5 | 1.0 | 1.0 | 1.5 | 2.1 | 1.7 | 1.5 | 1.2 | 1.6 | 1.9 |
Intensity Change (Log2) | – | – | – | 0.0 | 0.09 | -0.17 | -0.35 | -0.07 | -0.35 | -0.32 | -0.34 | -0.22 | 0.08 | 0.18 | 0.21 | 0.58 | 0.41 | 0.3 | 0.1 | 0.13 | 0.29 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.7 |
Bud | -0.6 | -1.0 | 0 | 0 | -0.5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -1.1 | 0 | 2.2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.8 | 1.6 | 4.5 |
Cytoplasm | 1.0 | 0.8 | 0.5 | 0 | 0.3 | -2.0 | -1.6 | -0.7 | 0.1 | 1.0 | 3.4 | 4.1 | 2.2 | 3.4 | 1.3 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.9 | 0 | 0 | 0 | 0 |
Endosome | -1.7 | 1.5 | 0 | 0 | -2.3 | 0 | 0 | 0 | 0 | 1.7 | 3.3 | 3.8 | 1.0 | 0.4 | 0.6 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.5 | 4.8 | 0 |
Mitochondria | 1.0 | -0.7 | 0 | 0 | 3.0 | 0 | 0 | 0 | 0 | -0.8 | -5.4 | -7.5 | -7.8 | -6.4 | -8.6 |
Nucleus | 0.7 | 0 | -0.5 | -2.1 | -0.1 | -1.2 | -1.5 | 0.2 | -0.9 | -2.9 | -3.7 | -5.0 | -2.4 | -2.5 | -2.2 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0.2 | 0.1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -0.1 | 0.9 | 2.7 | 1.3 | 1.4 | 0.8 |
SpindlePole | -0.8 | -2.2 | 0 | 0 | -1.6 | 0 | 0 | 0 | 0 | -1.8 | -2.6 | -3.8 | -0.8 | -0.8 | -1.1 |
Vacuole | -0.6 | 2.4 | 1.1 | 0.2 | 0.2 | 1.3 | -0.4 | 0.1 | 1.6 | 3.5 | 3.3 | 5.3 | 5.7 | 2.3 | 2.9 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.1797 | 1.2433 | 1.0192 | 0.8205 | 0.2014 | 1.0331 | 2.3131 | 1.7394 | 1.7493 | 1.4304 | 1.7445 | 1.6784 | 2.3089 | 1.8766 | 1.8433 | 1.9155 | 1.8844 | 1.8277 |
Actin | 0.0196 | 0.0042 | 0.0041 | 0.0017 | 0.0186 | 0.004 | 0.0151 | 0.0097 | 0.0037 | 0.0009 | 0.0039 | 0.0024 | 0.0114 | 0.0102 | 0.0086 | 0.0012 | 0.0006 | 0.0159 |
Bud | 0.0007 | 0.0047 | 0.0023 | 0.0006 | 0.0011 | 0.0007 | 0.0022 | 0.002 | 0.0024 | 0.0014 | 0.0013 | 0.0009 | 0.0028 | 0.0011 | 0.0015 | 0.0002 | 0.0001 | 0.0027 |
Bud Neck | 0.0005 | 0.0007 | 0.0003 | 0.0003 | 0.0037 | 0.0004 | 0.0017 | 0.0008 | 0.003 | 0.0002 | 0.0005 | 0.0007 | 0.0022 | 0.0004 | 0.0007 | 0 | 0.0001 | 0.0007 |
Bud Periphery | 0.0029 | 0.0036 | 0.0035 | 0.0017 | 0.0022 | 0.001 | 0.0021 | 0.004 | 0.004 | 0.0163 | 0.0035 | 0.0014 | 0.0024 | 0.0026 | 0.0026 | 0.0007 | 0.0003 | 0.0017 |
Bud Site | 0.0014 | 0.0212 | 0.0027 | 0.0004 | 0.0033 | 0.0009 | 0.0045 | 0.0135 | 0.005 | 0.0006 | 0.0022 | 0.001 | 0.003 | 0.004 | 0.0074 | 0.0005 | 0.0001 | 0.0004 |
Cell Periphery | 0.0004 | 0.0007 | 0.0007 | 0.0002 | 0.0019 | 0.0002 | 0.0015 | 0.0007 | 0.0005 | 0.0021 | 0.0003 | 0.0002 | 0.0007 | 0.0005 | 0.0007 | 0.0001 | 0.0001 | 0.0003 |
Cytoplasm | 0.0021 | 0.0034 | 0.0032 | 0.0015 | 0.002 | 0.004 | 0.0044 | 0.0034 | 0.0042 | 0.0017 | 0.0063 | 0.0015 | 0.0017 | 0.0053 | 0.0006 | 0.0001 | 0.0003 | 0.0022 |
Cytoplasmic Foci | 0.0206 | 0.0359 | 0.0176 | 0.0126 | 0.0183 | 0.0188 | 0.0275 | 0.0277 | 0.0202 | 0.0567 | 0.0314 | 0.0225 | 0.0242 | 0.0194 | 0.0112 | 0.0107 | 0.0141 | 0.0123 |
Eisosomes | 0.0004 | 0.0003 | 0.0003 | 0.0001 | 0.0024 | 0.0002 | 0.0015 | 0.0003 | 0.0002 | 0.0005 | 0.0004 | 0.0001 | 0.0004 | 0.0003 | 0.0004 | 0.0001 | 0.0001 | 0.0003 |
Endoplasmic Reticulum | 0.0021 | 0.0007 | 0.0027 | 0.0005 | 0.0006 | 0.0011 | 0.0018 | 0.0007 | 0.0008 | 0.0003 | 0.0043 | 0.0012 | 0.0012 | 0.0011 | 0.0002 | 0.0001 | 0.0003 | 0.0018 |
Endosome | 0.0748 | 0.0767 | 0.0424 | 0.0758 | 0.0199 | 0.0365 | 0.0807 | 0.0867 | 0.0516 | 0.0822 | 0.0536 | 0.0379 | 0.0519 | 0.0425 | 0.0294 | 0.0103 | 0.0384 | 0.0387 |
Golgi | 0.0407 | 0.0521 | 0.0259 | 0.0548 | 0.0212 | 0.0383 | 0.0551 | 0.0451 | 0.0296 | 0.0282 | 0.0355 | 0.0373 | 0.0509 | 0.0619 | 0.0391 | 0.0486 | 0.0441 | 0.0522 |
Lipid Particles | 0.057 | 0.0329 | 0.0197 | 0.0048 | 0.0332 | 0.0137 | 0.0429 | 0.0143 | 0.013 | 0.0185 | 0.0049 | 0.0135 | 0.018 | 0.0066 | 0.0037 | 0.0007 | 0.0006 | 0.0125 |
Mitochondria | 0.6992 | 0.7001 | 0.804 | 0.827 | 0.7951 | 0.8474 | 0.6978 | 0.7277 | 0.8174 | 0.7255 | 0.809 | 0.8235 | 0.7866 | 0.8064 | 0.8703 | 0.8928 | 0.8691 | 0.8313 |
None | 0.004 | 0.0026 | 0.0151 | 0.0003 | 0.0073 | 0.0007 | 0.006 | 0.0066 | 0.003 | 0.0025 | 0.0043 | 0.0017 | 0.0037 | 0.001 | 0.0002 | 0.0001 | 0.0001 | 0.0006 |
Nuclear Periphery | 0.0117 | 0.0005 | 0.0048 | 0.0001 | 0.0055 | 0.0024 | 0.0049 | 0.0005 | 0.0016 | 0.0003 | 0.0053 | 0.0042 | 0.002 | 0.0008 | 0.0001 | 0 | 0.0003 | 0.0007 |
Nucleolus | 0.0013 | 0.0011 | 0.0013 | 0.0001 | 0.0019 | 0.0002 | 0.003 | 0.0009 | 0.0011 | 0.0004 | 0.0006 | 0.0009 | 0.0012 | 0.0004 | 0.0008 | 0 | 0 | 0.0009 |
Nucleus | 0.0058 | 0.0007 | 0.0018 | 0.0002 | 0.0031 | 0.0004 | 0.0027 | 0.0007 | 0.0007 | 0.0002 | 0.0018 | 0.0013 | 0.0008 | 0.0008 | 0.0006 | 0 | 0 | 0.0023 |
Peroxisomes | 0.0396 | 0.0445 | 0.0392 | 0.0109 | 0.0392 | 0.0244 | 0.0283 | 0.0377 | 0.0295 | 0.0534 | 0.0245 | 0.0412 | 0.0171 | 0.0297 | 0.0165 | 0.0315 | 0.0031 | 0.0158 |
Punctate Nuclear | 0.0024 | 0.0006 | 0.0021 | 0 | 0.0144 | 0.0003 | 0.0057 | 0.0004 | 0.0016 | 0.0013 | 0.0008 | 0.0013 | 0.002 | 0.0001 | 0.0001 | 0 | 0 | 0.0004 |
Vacuole | 0.0071 | 0.008 | 0.0034 | 0.0028 | 0.0025 | 0.0022 | 0.0059 | 0.0114 | 0.0042 | 0.0042 | 0.0032 | 0.0019 | 0.0097 | 0.0024 | 0.003 | 0.0006 | 0.0034 | 0.0031 |
Vacuole Periphery | 0.0059 | 0.005 | 0.0029 | 0.0035 | 0.0026 | 0.0021 | 0.0046 | 0.0051 | 0.0027 | 0.0026 | 0.0023 | 0.0032 | 0.0058 | 0.0021 | 0.0024 | 0.0014 | 0.025 | 0.0033 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 2.8645 | 6.1456 | 4.5603 | 9.3675 | 12.6274 | 8.0162 | 13.7223 | 17.1252 | 17.5136 | 11.2695 |
Translational Efficiency | 1.4292 | 1.1868 | 1.9524 | 1.4198 | 0.4626 | 1.1067 | 0.7982 | 0.9067 | 0.8727 | 0.6894 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
154 | 535 | 1901 | 325 | 1513 | 2097 | 246 | 476 | 1667 | 2632 | 2147 | 801 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 733.85 | 762.84 | 863.53 | 916.68 | 701.35 | 794.87 | 1004.68 | 830.09 | 704.35 | 788.36 | 879.70 | 865.22 |
Standard Deviation | 80.01 | 107.71 | 104.03 | 106.40 | 83.58 | 101.30 | 112.66 | 118.13 | 83.79 | 103.44 | 114.27 | 121.22 |
Intensity Change Log 2 | — | 0.055895 | 0.234761 | 0.320933 | — | 0.180584 | 0.518530 | 0.243133 | — | 0.118175 | 0.380405 | 0.283438 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000879 | 0.004014 | 0.004311 | 0.028525 | 0.000990 | 0.000890 | 0.011726 | 0.003351 | 0.000980 | 0.001525 | 0.005161 | 0.013565 |
Bud Neck | 0.023684 | 0.013550 | 0.014862 | 0.011098 | 0.015841 | 0.004874 | 0.024200 | 0.030249 | 0.016566 | 0.006638 | 0.015932 | 0.022479 |
Bud Site | 0.012736 | 0.043072 | 0.062908 | 0.158966* | 0.024001 | 0.012340 | 0.073533 | 0.065344 | 0.022960 | 0.018587 | 0.064125 | 0.103331* |
Cell Periphery | 0.001153 | 0.001110 | 0.000183 | 0.001166 | 0.000562 | 0.000250 | 0.000397 | 0.000520 | 0.000617 | 0.000425 | 0.000208 | 0.000782 |
Cytoplasm | 0.015244 | 0.008766 | 0.043424 | 0.027671 | 0.028323 | 0.004588 | 0.035538 | 0.036320 | 0.027115 | 0.005437 | 0.042520 | 0.032811 |
Cytoplasmic Foci | 0.082340 | 0.049566 | 0.117544 | 0.104666 | 0.101749 | 0.041376 | 0.171650 | 0.091618 | 0.099956 | 0.043041 | 0.123744 | 0.096912 |
Eisosomes | 0.000167 | 0.000126 | 0.000094 | 0.000216 | 0.000211 | 0.000065 | 0.000244 | 0.000112 | 0.000207 | 0.000077 | 0.000111 | 0.000154 |
Endoplasmic Reticulum | 0.002725 | 0.000747 | 0.006751 | 0.005100 | 0.001476 | 0.000288 | 0.010299 | 0.002051 | 0.001591 | 0.000382 | 0.007157 | 0.003288 |
Endosome | 0.101319 | 0.092619 | 0.061014 | 0.096654 | 0.066562 | 0.080567 | 0.136764 | 0.146636 | 0.069773 | 0.083017 | 0.069693 | 0.126356 |
Golgi | 0.120035* | 0.119909* | 0.076341 | 0.108016 | 0.121893* | 0.130775* | 0.078854 | 0.056722 | 0.121722* | 0.128567* | 0.076629 | 0.077534 |
Lipid Particles | 0.010777 | 0.004194 | 0.002547 | 0.001585 | 0.007451 | 0.001910 | 0.011783 | 0.007550 | 0.007758 | 0.002374 | 0.003606 | 0.005130 |
Mitochondria | 0.505722* | 0.547385* | 0.249980* | 0.181260* | 0.542534* | 0.646942* | 0.057724 | 0.272269* | 0.539133* | 0.626705* | 0.227951* | 0.235342* |
Mitotic Spindle | 0.006826 | 0.014787 | 0.140939* | 0.065231 | 0.007373 | 0.013991 | 0.079372 | 0.056680 | 0.007322 | 0.014153 | 0.133885* | 0.060150 |
None | 0.000257 | 0.000461 | 0.000580 | 0.000186 | 0.000663 | 0.000293 | 0.000356 | 0.001277 | 0.000625 | 0.000328 | 0.000554 | 0.000834 |
Nuclear Periphery | 0.000436 | 0.000176 | 0.002029 | 0.000983 | 0.000120 | 0.000096 | 0.001789 | 0.000997 | 0.000149 | 0.000112 | 0.002001 | 0.000991 |
Nuclear Periphery Foci | 0.000401 | 0.000720 | 0.002506 | 0.001233 | 0.000243 | 0.000201 | 0.003288 | 0.001351 | 0.000258 | 0.000306 | 0.002596 | 0.001303 |
Nucleolus | 0.004929 | 0.002263 | 0.001787 | 0.000587 | 0.000948 | 0.000472 | 0.005172 | 0.001281 | 0.001316 | 0.000836 | 0.002175 | 0.000999 |
Nucleus | 0.004285 | 0.002120 | 0.016395 | 0.005316 | 0.004176 | 0.000629 | 0.030119 | 0.007358 | 0.004186 | 0.000932 | 0.017967 | 0.006529 |
Peroxisomes | 0.054288 | 0.053235 | 0.026344 | 0.101186* | 0.036448 | 0.020885 | 0.122605* | 0.050166 | 0.038096 | 0.027461 | 0.037373 | 0.070867 |
Vacuole | 0.021203 | 0.024029 | 0.127178 | 0.075204 | 0.019764 | 0.018597 | 0.120629 | 0.127227 | 0.019897 | 0.019701 | 0.126427 | 0.106119 |
Vacuole Periphery | 0.030592 | 0.017150 | 0.042284 | 0.025149 | 0.018672 | 0.019970 | 0.023960 | 0.040922 | 0.019773 | 0.019396 | 0.040184 | 0.034523 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -2.78 | -5.84 | -5.55 | -4.83 | -4.83 | 0.37 | -5.53 | -2.40 | -2.45 | 3.83 | -1.77 | -9.15 | -5.89 | -5.59 | -3.79 |
Bud Neck | 1.54 | 1.32 | 2.01 | 0.86 | 2.73 | 7.11 | -2.88 | -3.36 | -6.29 | -1.25 | 6.30 | -0.02 | -2.05 | -6.23 | -2.23 |
Bud Site | -4.96 | -11.98 | -12.52 | -9.47 | -8.37 | 5.43 | -7.02 | -6.80 | -8.97 | 0.99 | 1.99 | -14.56 | -13.06 | -13.96 | -6.10 |
Cell Periphery | 0.09 | 2.45 | -0.02 | -0.13 | -3.39 | 2.86 | 1.44 | 0.45 | -3.23 | -1.23 | 1.69 | 3.98 | -0.95 | -2.57 | -4.48 |
Cytoplasm | 1.10 | -5.04 | -2.04 | -5.17 | 4.23 | 9.90 | -1.74 | -2.02 | -7.03 | 0.07 | 9.61 | -5.74 | -1.98 | -9.01 | 2.54 |
Cytoplasmic Foci | 3.05 | -3.19 | -1.83 | -7.71 | 1.76 | 15.38 | -6.32 | 1.12 | -8.72 | 6.44 | 15.39 | -4.81 | 0.33 | -12.26 | 4.54 |
Eisosomes | 1.55 | 2.85 | -1.52 | -4.05 | -5.99 | 6.70 | -1.39 | 4.23 | -6.75 | 6.45 | 6.48 | 4.63 | 2.35 | -8.15 | -4.21 |
Endoplasmic Reticulum | 1.78 | -3.70 | -1.60 | -4.10 | 1.71 | 4.50 | -4.14 | -1.63 | -6.42 | 3.86 | 4.62 | -10.69 | -3.29 | -6.33 | 6.20 |
Endosome | 0.62 | 3.21 | 0.34 | -0.39 | -4.68 | -3.40 | -6.68 | -8.86 | -7.26 | -0.99 | -3.40 | 0.43 | -8.40 | -6.52 | -9.15 |
Golgi | 0.01 | 3.12 | 0.75 | 1.06 | -3.26 | -1.48 | 5.22 | 10.42 | 12.64 | 1.97 | -1.24 | 9.19 | 7.02 | 8.82 | -0.34 |
Lipid Particles | 1.75 | 2.45 | 2.78 | 1.43 | 2.36 | 4.70 | -2.06 | -0.17 | -2.31 | 1.34 | 4.65 | 3.59 | 1.29 | -1.84 | -0.97 |
Mitochondria | -1.25 | 8.74 | 10.04 | 17.47 | 4.18 | -9.16 | 40.49* | 16.25 | 23.67 | -12.43 | -8.14 | 30.40 | 22.82 | 32.29 | -0.55 |
Mitotic Spindle | -2.34 | -23.63* | -6.57 | -5.70 | 7.56 | -4.68 | -7.16 | -7.80 | -6.74 | 1.71 | -5.29 | -26.68 | -10.28 | -8.90 | 10.37 |
None | -2.75 | -6.63 | 1.82 | 4.09 | 10.67 | 1.76 | 1.13 | -0.65 | -1.10 | -0.95 | 1.57 | 0.32 | -0.33 | -0.94 | -0.47 |
Nuclear Periphery | 1.16 | -4.89 | -0.66 | -1.00 | 1.34 | 0.82 | -3.08 | -2.74 | -2.82 | 1.30 | 0.97 | -7.48 | -2.24 | -2.34 | 2.39 |
Nuclear Periphery Foci | -0.68 | -4.90 | -1.13 | -0.46 | 1.64 | 0.48 | -3.39 | -1.62 | -1.67 | 2.28 | -0.48 | -6.31 | -2.08 | -1.89 | 2.35 |
Nucleolus | 0.62 | 0.72 | 1.03 | 1.42 | 3.54 | 1.80 | -4.06 | -1.24 | -3.56 | 3.77 | 0.96 | -1.91 | 0.63 | -0.67 | 3.97 |
Nucleus | 1.13 | -6.07 | -0.34 | -1.10 | 3.95 | 4.56 | -5.15 | -2.38 | -4.90 | 4.35 | 4.43 | -9.64 | -1.77 | -4.10 | 6.18 |
Peroxisomes | 0.09 | 3.01 | -3.29 | -3.89 | -6.79 | 5.33 | -7.35 | -2.42 | -5.47 | 5.70 | 3.62 | 0.27 | -5.51 | -7.63 | -5.78 |
Vacuole | -0.46 | -16.40 | -5.96 | -6.56 | 6.47 | 0.74 | -9.46 | -13.23 | -13.42 | -0.28 | 0.15 | -28.95 | -14.81 | -14.97 | 2.76 |
Vacuole Periphery | 2.27 | -1.79 | 0.81 | -1.90 | 3.83 | -0.88 | -0.89 | -5.23 | -4.97 | -3.06 | 0.25 | -9.23 | -4.63 | -4.96 | 1.71 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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