Standard name | |
---|---|
Human Ortholog | |
Description | Phosphopantothenoylcysteine synthetase (PPCS); catalyzes the second step of coenzyme A biosynthesis from pantothenate; subunit of the CoA-Synthesizing Protein Complex (CoA-SPC) that contains: Cab2p, Cab3p, Cab4p, Cab5p, Sis2p and Vhs3p subunits; null mutant lethality is complemented by human homolog PPCS and by E. coli coaBC, a bifunctional enzyme with PPCS activity |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 3 | 3 |
Bud | 0 | 1 | 0 | 1 | 0 | 0 | 3 | 6 | 4 | 7 | 7 | 9 | 2 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 3 | 16 | 21 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 3 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 2 | 1 | 4 | 4 | 7 | 4 | 5 | 7 | 4 | 6 | 8 | 1 | 1 | 2 | 22 | 20 | 13 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 172 | 190 | 174 | 288 | 472 | 322 | 430 | 465 | 264 | 314 | 229 | 251 | 422 | 546 | 507 | 362 | 309 | 276 | 167 | 183 | 173 | 323 | 487 | 554 |
Endoplasmic Reticulum | 1 | 2 | 1 | 1 | 0 | 0 | 4 | 5 | 4 | 2 | 3 | 5 | 1 | 1 | 0 | 16 | 7 | 7 | 1 | 1 | 2 | 3 | 2 | 4 |
Endosome | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 2 | 2 | 2 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 1 | 1 | 2 |
Mitochondria | 4 | 0 | 0 | 3 | 2 | 32 | 68 | 70 | 102 | 71 | 100 | 65 | 1 | 0 | 0 | 2 | 0 | 2 | 1 | 0 | 1 | 1 | 3 | 2 |
Nucleus | 0 | 0 | 1 | 3 | 6 | 0 | 11 | 14 | 6 | 9 | 4 | 12 | 0 | 0 | 1 | 3 | 0 | 2 | 0 | 0 | 0 | 2 | 2 | 1 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 9 | 7 |
Vac/Vac Membrane | 1 | 0 | 0 | 3 | 14 | 10 | 24 | 37 | 26 | 50 | 12 | 37 | 3 | 4 | 1 | 22 | 30 | 10 | 1 | 1 | 0 | 3 | 3 | 2 |
Unique Cell Count | 177 | 192 | 174 | 292 | 487 | 347 | 498 | 552 | 352 | 410 | 314 | 347 | 426 | 550 | 508 | 387 | 331 | 287 | 181 | 190 | 185 | 348 | 542 | 614 |
Labelled Cell Count | 179 | 195 | 177 | 304 | 499 | 372 | 544 | 602 | 413 | 459 | 363 | 390 | 430 | 553 | 512 | 429 | 367 | 311 | 181 | 190 | 185 | 348 | 542 | 614 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.2 | 7.8 | 7.3 | 6.7 | 7.2 | 5.8 | 5.9 | 6.2 | 5.6 | 6.0 | 5.4 | 6.2 | 8.0 | 8.5 | 8.4 | 12.1 | 13.9 | 13.7 | 7.6 | 8.7 | 10.3 |
Std Deviation (1e-4) | 1.0 | 1.2 | 1.3 | 1.2 | 1.4 | 1.4 | 1.5 | 1.3 | 1.3 | 1.4 | 1.3 | 1.7 | 1.1 | 1.3 | 1.5 | 2.5 | 3.2 | 3.0 | 1.8 | 2.3 | 2.7 |
Intensity Change (Log2) | – | – | – | -0.12 | -0.02 | -0.33 | -0.31 | -0.23 | -0.4 | -0.29 | -0.44 | -0.25 | 0.12 | 0.22 | 0.2 | 0.73 | 0.93 | 0.9 | 0.06 | 0.24 | 0.49 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.8 | 2.9 | 2.4 |
Cytoplasm | -1.6 | -2.3 | -3.6 | -5.1 | -5.6 | -7.2 | -7.0 | -7.6 | -7.7 | -1.3 | -1.1 | -0.6 | -3.4 | -3.5 | -2.6 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.3 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 4.1 | 5.1 | 4.9 | 7.9 | 5.9 | 8.3 | 6.1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 2.9 | 3.5 | 3.7 | 4.8 | 0 | 4.5 | 0 | 0 | 0 | 3.2 | 4.1 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 4.3019 | 5.1999 | 4.963 | 4.629 | 4.1343 | 4.8362 | 3.8133 | 5.3477 | 4.628 | 4.6752 | 4.1397 | 4.5943 | 3.3111 | 5.226 | 4.3561 | 3.6686 | 3.1131 | 4.0715 |
Actin | 0.0021 | 0.0006 | 0.0026 | 0.0002 | 0.0021 | 0.0007 | 0.0076 | 0.001 | 0.0026 | 0.0024 | 0.003 | 0.0011 | 0.0406 | 0.0002 | 0.0036 | 0.108 | 0.0118 | 0.0102 |
Bud | 0.0003 | 0.0008 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0012 | 0.0001 | 0.0028 | 0.0001 | 0.015 | 0.0022 | 0.0007 | 0.0001 | 0.0003 | 0.0002 | 0.0006 | 0.0024 |
Bud Neck | 0.0004 | 0.0004 | 0.0003 | 0.0004 | 0.0004 | 0.0009 | 0.0028 | 0.0002 | 0.0018 | 0.0004 | 0.0006 | 0.002 | 0.0007 | 0.0001 | 0.0005 | 0.0005 | 0.0003 | 0.0014 |
Bud Periphery | 0.0003 | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0011 | 0 | 0.0008 | 0 | 0.0058 | 0.0012 | 0.0005 | 0 | 0.0002 | 0.0001 | 0.0008 | 0.0013 |
Bud Site | 0.0005 | 0.0047 | 0.0005 | 0.0001 | 0.0001 | 0.0001 | 0.0023 | 0.0005 | 0.0031 | 0.0003 | 0.0011 | 0.0008 | 0.0035 | 0.0002 | 0.0014 | 0.0002 | 0.0005 | 0.0005 |
Cell Periphery | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0.0002 | 0.0001 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0002 | 0.0001 |
Cytoplasm | 0.892 | 0.9325 | 0.9151 | 0.9594 | 0.9121 | 0.9511 | 0.8072 | 0.9546 | 0.8864 | 0.8444 | 0.8597 | 0.9187 | 0.6286 | 0.9842 | 0.9203 | 0.8367 | 0.335 | 0.9165 |
Cytoplasmic Foci | 0.0169 | 0.0039 | 0.0076 | 0.0062 | 0.01 | 0.0111 | 0.0233 | 0.0042 | 0.0164 | 0.066 | 0.037 | 0.0145 | 0.0585 | 0.0012 | 0.0193 | 0.004 | 0.0538 | 0.0128 |
Eisosomes | 0 | 0 | 0.0002 | 0 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0.0004 | 0 | 0 | 0.0001 | 0.0004 | 0 |
Endoplasmic Reticulum | 0.0036 | 0.0016 | 0.0016 | 0.0007 | 0.0027 | 0.0014 | 0.0043 | 0.0009 | 0.0016 | 0.0009 | 0.003 | 0.0016 | 0.006 | 0.0008 | 0.0022 | 0.0059 | 0.0157 | 0.0051 |
Endosome | 0.0095 | 0.0014 | 0.0027 | 0.0013 | 0.0055 | 0.0058 | 0.0307 | 0.0013 | 0.0108 | 0.0054 | 0.0314 | 0.0068 | 0.0952 | 0.0004 | 0.01 | 0.0044 | 0.2861 | 0.0156 |
Golgi | 0.001 | 0.0002 | 0.0003 | 0.0001 | 0.0019 | 0.0003 | 0.0086 | 0.0001 | 0.0032 | 0.0032 | 0.0051 | 0.0014 | 0.0397 | 0 | 0.0046 | 0.0304 | 0.1191 | 0.0142 |
Lipid Particles | 0.0005 | 0 | 0 | 0 | 0.0159 | 0.0001 | 0.0145 | 0 | 0.0023 | 0.0075 | 0.0042 | 0.0015 | 0.0236 | 0 | 0.0004 | 0.0003 | 0.0314 | 0.0002 |
Mitochondria | 0.0008 | 0.0002 | 0.0003 | 0.0002 | 0.0008 | 0.0005 | 0.0061 | 0.0001 | 0.0067 | 0.0003 | 0.0006 | 0.0055 | 0.0057 | 0 | 0.0018 | 0.0005 | 0.0132 | 0.0073 |
None | 0.0572 | 0.0494 | 0.0555 | 0.0245 | 0.033 | 0.0207 | 0.0552 | 0.0324 | 0.0385 | 0.0229 | 0.0145 | 0.0266 | 0.0683 | 0.011 | 0.0284 | 0.0026 | 0.0886 | 0.0042 |
Nuclear Periphery | 0.0045 | 0.0006 | 0.0014 | 0.0005 | 0.0022 | 0.0006 | 0.0118 | 0.0004 | 0.0018 | 0.0008 | 0.0024 | 0.0029 | 0.004 | 0.0003 | 0.0007 | 0.0013 | 0.0151 | 0.0013 |
Nucleolus | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0008 | 0 | 0.0013 | 0 | 0.0001 | 0.0001 | 0.0004 | 0 | 0 | 0 | 0.0003 | 0 |
Nucleus | 0.0058 | 0.0023 | 0.0084 | 0.0049 | 0.0054 | 0.0041 | 0.0068 | 0.0033 | 0.0082 | 0.0041 | 0.0045 | 0.0066 | 0.0033 | 0.0009 | 0.0034 | 0.0027 | 0.0042 | 0.004 |
Peroxisomes | 0.0007 | 0.0001 | 0.0003 | 0.0001 | 0.0035 | 0.0005 | 0.0078 | 0.0001 | 0.0056 | 0.0371 | 0.0056 | 0.0037 | 0.0067 | 0 | 0.0016 | 0.0002 | 0.0068 | 0.0006 |
Punctate Nuclear | 0.0022 | 0.0004 | 0.002 | 0.0006 | 0.0028 | 0.0007 | 0.0031 | 0.0003 | 0.0044 | 0.0032 | 0.0054 | 0.0011 | 0.0093 | 0.0001 | 0.0004 | 0.0002 | 0.003 | 0.0013 |
Vacuole | 0.0009 | 0.0004 | 0.0006 | 0.0004 | 0.0008 | 0.0008 | 0.0027 | 0.0003 | 0.0011 | 0.0007 | 0.0008 | 0.0011 | 0.0031 | 0.0003 | 0.0006 | 0.0014 | 0.0065 | 0.0007 |
Vacuole Periphery | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0.0002 | 0.0021 | 0 | 0.0003 | 0.0001 | 0.0002 | 0.0005 | 0.0012 | 0 | 0.0002 | 0.0002 | 0.0065 | 0.0003 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 35.6747 | 22.4585 | 28.6641 | 27.2204 | 18.8924 | 35.1528 | 28.3847 | 28.2002 | 21.9204 | 33.3917 |
Translational Efficiency | 1.7278 | 2.1583 | 1.293 | 1.3083 | 2.0181 | 1.6384 | 1.6756 | 1.324 | 1.6392 | 1.3905 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
31 | 395 | 1935 | 1160 | 1421 | 1323 | 1272 | 1145 | 1452 | 1718 | 3207 | 2305 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1022.71 | 1031.64 | 1125.57 | 1246.74 | 840.24 | 967.71 | 1151.01 | 1263.15 | 844.14 | 982.41 | 1135.66 | 1254.89 |
Standard Deviation | 317.13 | 144.94 | 154.33 | 183.72 | 129.73 | 163.54 | 156.15 | 222.75 | 138.97 | 161.71 | 155.56 | 204.21 |
Intensity Change Log 2 | — | 0.012543 | 0.138259 | 0.285764 | — | 0.203773 | 0.454027 | 0.588153 | — | 0.101942 | 0.289279 | 0.430035 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.003346 | 0.000414 | 0.000163 | 0.004920 | 0.000465 | 0.000453 | 0.000237 | 0.001492 | 0.000527 | 0.000444 | 0.000192 | 0.003217 |
Bud Neck | 0.005154 | 0.002286 | 0.000910 | 0.003637 | 0.001805 | 0.003748 | 0.000967 | 0.003491 | 0.001876 | 0.003412 | 0.000933 | 0.003565 |
Bud Site | 0.082646 | 0.016695 | 0.004744 | 0.029403 | 0.006010 | 0.013874 | 0.002687 | 0.034461 | 0.007646 | 0.014523 | 0.003928 | 0.031916 |
Cell Periphery | 0.000719 | 0.000113 | 0.000056 | 0.000121 | 0.000092 | 0.000077 | 0.000112 | 0.000130 | 0.000105 | 0.000085 | 0.000078 | 0.000125 |
Cytoplasm | 0.516622* | 0.715207* | 0.763417* | 0.681136* | 0.762094* | 0.729235* | 0.671513* | 0.725675* | 0.756853* | 0.726010* | 0.726965* | 0.703261* |
Cytoplasmic Foci | 0.195369* | 0.139745 | 0.009838 | 0.018468 | 0.096341 | 0.109915 | 0.019450 | 0.029899 | 0.098455 | 0.116774 | 0.013650 | 0.024146 |
Eisosomes | 0.000068 | 0.000025 | 0.000006 | 0.000015 | 0.000026 | 0.000031 | 0.000029 | 0.000014 | 0.000027 | 0.000030 | 0.000015 | 0.000015 |
Endoplasmic Reticulum | 0.007938 | 0.001724 | 0.000679 | 0.000588 | 0.001370 | 0.000527 | 0.001436 | 0.001013 | 0.001510 | 0.000802 | 0.000980 | 0.000799 |
Endosome | 0.015667 | 0.007460 | 0.000983 | 0.005162 | 0.005090 | 0.007491 | 0.002508 | 0.009966 | 0.005316 | 0.007483 | 0.001588 | 0.007549 |
Golgi | 0.011116 | 0.002995 | 0.000444 | 0.008411 | 0.001459 | 0.002604 | 0.000093 | 0.004577 | 0.001665 | 0.002694 | 0.000304 | 0.006506 |
Lipid Particles | 0.033887 | 0.001156 | 0.000364 | 0.000912 | 0.001312 | 0.001397 | 0.001040 | 0.000959 | 0.002008 | 0.001341 | 0.000632 | 0.000936 |
Mitochondria | 0.011522 | 0.000238 | 0.000262 | 0.003988 | 0.002029 | 0.001537 | 0.000121 | 0.002456 | 0.002232 | 0.001238 | 0.000206 | 0.003227 |
Mitotic Spindle | 0.009223 | 0.004664 | 0.002468 | 0.038988 | 0.005403 | 0.009369 | 0.002308 | 0.040297 | 0.005485 | 0.008287 | 0.002405 | 0.039639 |
None | 0.003047 | 0.001061 | 0.005855 | 0.005623 | 0.003939 | 0.002364 | 0.007427 | 0.006595 | 0.003920 | 0.002064 | 0.006478 | 0.006106 |
Nuclear Periphery | 0.000585 | 0.000111 | 0.000355 | 0.000381 | 0.000229 | 0.000102 | 0.000576 | 0.000399 | 0.000237 | 0.000104 | 0.000443 | 0.000390 |
Nuclear Periphery Foci | 0.010189 | 0.003637 | 0.002379 | 0.003327 | 0.002218 | 0.001219 | 0.003848 | 0.002977 | 0.002388 | 0.001775 | 0.002961 | 0.003153 |
Nucleolus | 0.002056 | 0.000150 | 0.000086 | 0.000169 | 0.000567 | 0.000395 | 0.000256 | 0.000094 | 0.000599 | 0.000339 | 0.000153 | 0.000132 |
Nucleus | 0.034849 | 0.030675 | 0.179959* | 0.106804 | 0.061225 | 0.042059 | 0.161819* | 0.067975 | 0.060662 | 0.039441 | 0.172764* | 0.087516 |
Peroxisomes | 0.003421 | 0.001356 | 0.000204 | 0.000982 | 0.000696 | 0.001933 | 0.000216 | 0.001409 | 0.000754 | 0.001800 | 0.000209 | 0.001194 |
Vacuole | 0.041314 | 0.069631 | 0.026740 | 0.081887 | 0.046059 | 0.071150 | 0.123245 | 0.064151 | 0.045957 | 0.070801 | 0.065017 | 0.073077 |
Vacuole Periphery | 0.011260 | 0.000656 | 0.000087 | 0.005077 | 0.001571 | 0.000523 | 0.000111 | 0.001970 | 0.001778 | 0.000554 | 0.000097 | 0.003534 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 2.68 | 2.86 | 1.47 | -1.37 | -1.63 | 0.08 | 0.33 | -0.88 | -1.91 | -1.76 | 0.38 | 1.03 | -1.23 | -1.63 | -2.03 |
Bud Neck | 1.63 | 2.28 | 1.50 | -0.54 | -4.15 | -3.45 | 2.45 | -1.44 | 2.16 | -3.09 | -3.36 | 3.14 | -1.84 | 2.05 | -5.10 |
Bud Site | 2.65 | 3.15 | 2.84 | 1.78 | -4.31 | -4.31 | 3.01 | -4.97 | -0.62 | -6.77 | -4.16 | 3.24 | -4.05 | 0.43 | -7.56 |
Cell Periphery | 2.71 | 2.90 | 2.73 | 0.37 | -4.82 | 2.19 | -1.66 | -2.22 | -4.12 | -0.74 | 2.44 | 3.03 | -0.23 | -3.46 | -4.64 |
Cytoplasm | -2.97 | -3.17 | -1.35 | 7.13 | 12.95 | 4.08 | 16.78 | 12.96 | 8.36 | -2.85 | 4.19 | 11.50 | 17.17 | 12.33 | 7.99 |
Cytoplasmic Foci | 1.33 | 4.53 | 4.45 | 16.02 | -3.09 | -2.55 | 22.95 | 21.03 | 21.53 | -2.89 | -3.58 | 24.64 | 23.12 | 27.45 | -4.86 |
Eisosomes | 2.56 | 3.68 | 3.24 | 4.44 | -7.19 | -1.64 | -1.30 | 4.60 | 8.05 | 9.17 | -1.03 | 4.25 | 4.99 | 9.04 | 1.95 |
Endoplasmic Reticulum | 2.11 | 2.43 | 2.54 | 2.34 | 4.79 | 4.65 | -0.37 | 5.13 | 0.75 | 8.57 | 3.33 | 2.45 | 5.86 | 2.30 | 9.12 |
Endosome | 1.25 | 2.26 | 2.15 | 6.23 | -2.00 | -2.90 | 5.89 | 3.67 | 6.01 | -0.47 | -2.84 | 7.73 | 5.66 | 8.87 | -1.81 |
Golgi | 1.94 | 2.76 | 2.40 | 0.67 | -1.79 | -1.68 | 4.29 | 1.17 | 2.51 | -2.64 | -1.41 | 4.27 | 0.81 | 1.93 | -2.63 |
Lipid Particles | 1.08 | 1.10 | 1.07 | -1.87 | -8.07 | -0.26 | 1.30 | -1.02 | -0.39 | -3.77 | 0.95 | 1.88 | 0.70 | -0.85 | -9.29 |
Mitochondria | 1.30 | 1.30 | 1.23 | -1.49 | -1.47 | 0.59 | 2.60 | 1.85 | 1.46 | -1.18 | 1.22 | 2.75 | 1.94 | 0.97 | -1.84 |
Mitotic Spindle | 0.44 | 1.16 | -0.87 | -2.07 | -4.90 | -1.38 | 0.84 | -3.72 | -2.37 | -4.70 | -1.23 | 1.43 | -4.25 | -2.90 | -6.87 |
None | 1.24 | -0.91 | -0.41 | -10.50 | 2.91 | 3.34 | -3.29 | -2.58 | -6.45 | 1.11 | 4.24 | -3.79 | -2.06 | -8.92 | 2.33 |
Nuclear Periphery | 1.09 | 0.09 | -0.28 | -18.32 | -4.52 | 2.18 | -9.81 | -4.35 | -10.67 | 6.72 | 2.31 | -7.43 | -6.26 | -20.30 | 1.80 |
Nuclear Periphery Foci | 1.63 | 1.89 | 1.77 | 1.05 | -1.49 | 4.18 | -6.33 | -2.21 | -6.01 | 4.10 | 2.29 | -3.13 | -2.26 | -5.10 | 1.12 |
Nucleolus | 1.51 | 1.54 | 1.48 | -1.25 | -3.93 | 0.90 | 1.61 | 1.83 | 1.58 | 4.27 | 1.19 | 1.89 | 1.86 | 1.43 | -0.71 |
Nucleus | 0.34 | -11.87 | -6.22 | -19.92 | 15.02 | 6.86 | -23.64 | -9.00 | -15.38 | 15.97 | 8.10 | -36.35 | -15.03 | -24.79 | 22.51 |
Peroxisomes | 2.04 | 3.39 | 3.25 | 4.28 | -1.53 | -4.04 | 2.13 | 2.06 | 5.61 | -0.64 | -3.99 | 3.91 | 3.14 | 6.90 | -1.47 |
Vacuole | -2.21 | -0.26 | -10.99 | -14.65 | -22.52 | -7.66 | -19.29 | -21.32 | -16.06 | -2.80 | -8.46 | -13.78 | -29.92 | -22.70 | -19.05 |
Vacuole Periphery | 1.72 | 1.81 | 1.66 | -0.50 | -1.33 | 3.14 | 4.50 | 3.19 | 0.54 | -2.11 | 3.48 | 4.86 | 2.21 | -0.45 | -1.77 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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