Standard name | |
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Human Ortholog | |
Description | Protein of unknown function; mitochondrial protein that physically interacts with Tim23p; null mutant displays reduced respiratory growth |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 2 | 0 | 3 | 3 | 3 | 5 |
Bud | 0 | 0 | 3 | 1 | 3 | 3 | – | 11 | 9 | 11 | 11 | 15 | 1 | 2 | 1 | 1 | 2 | 0 | 1 | 1 | 0 | 0 | 9 | 3 |
Bud Neck | 1 | 0 | 2 | 0 | 0 | 0 | – | 1 | 0 | 2 | 0 | 0 | 1 | 0 | 0 | 1 | 2 | 0 | 2 | 1 | 0 | 1 | 2 | 2 |
Bud Site | 0 | 0 | 0 | 0 | 1 | 1 | – | 3 | 5 | 5 | 2 | 10 | 0 | 1 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 1 | 0 | 1 | – | 0 | 0 | 1 | 1 | 1 | 1 | 0 | 5 | 3 | 0 | 0 | 2 | 0 | 1 | 1 | 0 | 5 |
Cytoplasm | 252 | 60 | 108 | 89 | 74 | 88 | – | 77 | 46 | 67 | 48 | 56 | 235 | 316 | 292 | 57 | 33 | 18 | 217 | 43 | 55 | 121 | 163 | 183 |
Endoplasmic Reticulum | 2 | 1 | 1 | 0 | 0 | 0 | – | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 1 | 4 | 3 | 1 | 0 | 0 | 4 | 1 | 1 | 1 |
Endosome | 1 | 0 | 0 | 0 | 4 | 2 | – | 1 | 0 | 1 | 0 | 0 | 1 | 1 | 3 | 8 | 2 | 2 | 3 | 4 | 4 | 2 | 2 | 2 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 2 | 0 | 3 | 3 | 2 |
Mitochondria | 13 | 12 | 16 | 34 | 46 | 107 | – | 173 | 168 | 206 | 177 | 226 | 3 | 7 | 5 | 2 | 1 | 2 | 27 | 19 | 42 | 43 | 43 | 25 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | – | 2 | 3 | 1 | 3 | 3 | 1 | 1 | 0 | 1 | 1 | 2 | 0 | 0 | 0 | 1 | 1 | 1 |
Nuclear Periphery | 0 | 1 | 0 | 0 | 0 | 0 | – | 0 | 4 | 2 | 4 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Nucleolus | 0 | 0 | 1 | 0 | 0 | 0 | – | 1 | 2 | 2 | 1 | 3 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 3 | 1 | – | 3 | 1 | 0 | 2 | 3 | 0 | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 |
SpindlePole | 0 | 0 | 1 | 0 | 1 | 6 | – | 17 | 4 | 9 | 11 | 19 | 1 | 3 | 1 | 0 | 0 | 2 | 3 | 1 | 1 | 3 | 5 | 5 |
Vac/Vac Membrane | 0 | 0 | 0 | 0 | 3 | 8 | – | 6 | 4 | 5 | 3 | 5 | 4 | 4 | 5 | 5 | 1 | 1 | 5 | 2 | 5 | 9 | 13 | 14 |
Unique Cell Count | 265 | 67 | 121 | 100 | 109 | 171 | 241 | 205 | 252 | 209 | 268 | 242 | 330 | 301 | 72 | 42 | 24 | 273 | 79 | 124 | 202 | 260 | 262 | |
Labelled Cell Count | 269 | 74 | 132 | 125 | 135 | 217 | 296 | 247 | 312 | 264 | 344 | 249 | 340 | 314 | 83 | 47 | 29 | 273 | 79 | 124 | 202 | 260 | 262 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.4 | 3.9 | 3.0 | 2.9 | 4.1 | 2.8 | – | 3.2 | 2.7 | 3.0 | 2.9 | 2.8 | 4.3 | 4.4 | 4.3 | 5.7 | 5.4 | 5.4 | 4.3 | 4.4 | 4.5 |
Std Deviation (1e-4) | 0.8 | 0.8 | 0.7 | 1.2 | 2.3 | 0.8 | – | 1.8 | 1.1 | 1.4 | 1.5 | 0.9 | 1.0 | 1.2 | 1.2 | 1.7 | 1.4 | 1.5 | 1.6 | 1.5 | 1.5 |
Intensity Change (Log2) | – | – | – | -0.06 | 0.45 | -0.1 | – | 0.1 | -0.18 | -0.02 | -0.04 | -0.14 | 0.5 | 0.53 | 0.52 | 0.92 | 0.83 | 0.84 | 0.5 | 0.55 | 0.59 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | -0.8852 | -0.5208 | -0.6351 | -0.7142 | -0.8126 | -0.5214 | -0.7103 | -0.2948 | -1.296 | 1.4813 | 0.1255 | -0.4445 | -0.1642 | 0.1177 | 0.1024 | 0.5339 | 0.091 | 0.1323 |
Actin | 0.0133 | 0.0045 | 0.0225 | 0.0008 | 0.0107 | 0.0014 | 0.0218 | 0.0058 | 0.0007 | 0.0009 | 0.0052 | 0.0018 | 0.0121 | 0.0019 | 0.0003 | 0.0012 | 0.0004 | 0.0018 |
Bud | 0.0007 | 0.0036 | 0.0008 | 0.0024 | 0.0026 | 0.0002 | 0.0016 | 0.019 | 0.0075 | 0.0036 | 0.0003 | 0.0017 | 0.0004 | 0.0004 | 0.0003 | 0.0018 | 0.0002 | 0.0001 |
Bud Neck | 0.0018 | 0.0009 | 0.0008 | 0.0007 | 0.0021 | 0.0017 | 0.0014 | 0.0003 | 0.0099 | 0.0002 | 0.0004 | 0.0095 | 0.004 | 0.0003 | 0.0006 | 0.0004 | 0 | 0.001 |
Bud Periphery | 0.0037 | 0.0049 | 0.0013 | 0.0058 | 0.01 | 0.0005 | 0.0053 | 0.0078 | 0.0041 | 0.0116 | 0.0005 | 0.0009 | 0.0009 | 0.0012 | 0.0009 | 0.0037 | 0.0007 | 0.0004 |
Bud Site | 0.0013 | 0.0264 | 0.0019 | 0.0011 | 0.0014 | 0.0013 | 0.0123 | 0.0197 | 0.0122 | 0.0006 | 0.001 | 0.0006 | 0.0028 | 0.0042 | 0.0006 | 0.0016 | 0 | 0.0002 |
Cell Periphery | 0.0008 | 0.0009 | 0.0003 | 0.0013 | 0.001 | 0.0003 | 0.001 | 0.0015 | 0.0008 | 0.0012 | 0.0001 | 0.0006 | 0.0009 | 0.001 | 0.0004 | 0.0011 | 0.0001 | 0.0002 |
Cytoplasm | 0.073 | 0.1058 | 0.076 | 0.1034 | 0.0699 | 0.1432 | 0.0645 | 0.1091 | 0.0693 | 0.0119 | 0.0585 | 0.1426 | 0.0586 | 0.0959 | 0.0849 | 0.139 | 0.0791 | 0.1302 |
Cytoplasmic Foci | 0.1032 | 0.0705 | 0.0518 | 0.0291 | 0.057 | 0.0532 | 0.0439 | 0.0188 | 0.0256 | 0.0277 | 0.098 | 0.0239 | 0.0521 | 0.0495 | 0.0194 | 0.0469 | 0.0101 | 0.0362 |
Eisosomes | 0.0005 | 0.0004 | 0.0008 | 0.0005 | 0.0019 | 0.0002 | 0.001 | 0.0006 | 0.0003 | 0.0002 | 0.0003 | 0.0002 | 0.0006 | 0.0005 | 0.0003 | 0.0004 | 0.0001 | 0.0001 |
Endoplasmic Reticulum | 0.0029 | 0.0025 | 0.0023 | 0.0025 | 0.0032 | 0.0023 | 0.0043 | 0.0038 | 0.0018 | 0.0021 | 0.0017 | 0.0014 | 0.0045 | 0.0022 | 0.0037 | 0.0014 | 0.0013 | 0.0022 |
Endosome | 0.06 | 0.0321 | 0.0323 | 0.0447 | 0.019 | 0.0242 | 0.044 | 0.016 | 0.011 | 0.0211 | 0.105 | 0.002 | 0.0297 | 0.0168 | 0.0122 | 0.0156 | 0.0018 | 0.012 |
Golgi | 0.0074 | 0.0078 | 0.0073 | 0.0018 | 0.0067 | 0.0049 | 0.0114 | 0.0031 | 0.0023 | 0.0113 | 0.0562 | 0.0005 | 0.0071 | 0.0047 | 0.0021 | 0.0032 | 0.0002 | 0.0012 |
Lipid Particles | 0.0255 | 0.0031 | 0.0115 | 0.0045 | 0.0402 | 0.0086 | 0.0114 | 0.0013 | 0.0028 | 0.0035 | 0.0394 | 0.0035 | 0.0143 | 0.0088 | 0.0035 | 0.0035 | 0.0002 | 0.0028 |
Mitochondria | 0.0278 | 0.0076 | 0.0444 | 0.0978 | 0.0081 | 0.0525 | 0.1065 | 0.0533 | 0.0173 | 0.2343 | 0.1238 | 0.042 | 0.0129 | 0.0176 | 0.0054 | 0.0068 | 0.0004 | 0.0102 |
None | 0.6432 | 0.7103 | 0.731 | 0.6758 | 0.7248 | 0.6851 | 0.5966 | 0.7165 | 0.8248 | 0.6332 | 0.4574 | 0.7533 | 0.7772 | 0.7808 | 0.852 | 0.7614 | 0.9035 | 0.7848 |
Nuclear Periphery | 0.0022 | 0.0006 | 0.001 | 0.0011 | 0.0051 | 0.002 | 0.0139 | 0.001 | 0.0007 | 0.0013 | 0.0006 | 0.0004 | 0.003 | 0.0013 | 0.0012 | 0.0006 | 0.0003 | 0.0006 |
Nucleolus | 0.0009 | 0.0009 | 0.0005 | 0.0012 | 0.0015 | 0.0006 | 0.0013 | 0.0012 | 0.0005 | 0.0004 | 0.0002 | 0.0008 | 0.0009 | 0.0005 | 0.0004 | 0.0003 | 0 | 0.0002 |
Nucleus | 0.0012 | 0.0013 | 0.0009 | 0.0037 | 0.0012 | 0.0013 | 0.0047 | 0.0138 | 0.0013 | 0.0007 | 0.0003 | 0.0011 | 0.0012 | 0.0011 | 0.0008 | 0.0006 | 0.0002 | 0.0007 |
Peroxisomes | 0.0114 | 0.0091 | 0.008 | 0.0042 | 0.021 | 0.0104 | 0.0085 | 0.0011 | 0.0012 | 0.0229 | 0.0445 | 0.0021 | 0.0077 | 0.0061 | 0.0073 | 0.0046 | 0.0006 | 0.0117 |
Punctate Nuclear | 0.0059 | 0.0016 | 0.0012 | 0.0017 | 0.0083 | 0.0017 | 0.0372 | 0.0022 | 0.0025 | 0.0016 | 0.0012 | 0.0104 | 0.0028 | 0.0016 | 0.0005 | 0.0007 | 0.0001 | 0.0012 |
Vacuole | 0.0113 | 0.0044 | 0.0025 | 0.0139 | 0.003 | 0.0032 | 0.0044 | 0.0033 | 0.003 | 0.0056 | 0.0031 | 0.0005 | 0.0055 | 0.0028 | 0.0027 | 0.0043 | 0.0004 | 0.0018 |
Vacuole Periphery | 0.0017 | 0.0008 | 0.0009 | 0.002 | 0.0011 | 0.0013 | 0.003 | 0.0007 | 0.0008 | 0.004 | 0.0023 | 0.0003 | 0.001 | 0.0008 | 0.0005 | 0.001 | 0.0001 | 0.0006 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 18.6229 | 4.1619 | 1.5442 | 11.9208 | 4.5273 | 23.2657 | 15.7804 | 24.8517 | 13.343 | 15.9651 |
Translational Efficiency | – | – | – | – | – | – | – | – | – | – |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | – |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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