Standard name | |
---|---|
Human Ortholog | |
Description | Subunit of GID complex; involved in proteasome-dependent catabolite inactivation of gluconeogenic enzymes FBPase, PEPCK, and c-MDH; forms dimer with Rmd5p that is then recruited to GID Complex by Gid8p; contains a degenerate RING finger motif needed for GID complex ubiquitin ligase activity in vivo, as well as CTLH and CRA domains; plays role in anti-apoptosis; required for survival upon exposure to K1 killer toxin |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 1 | 4 | 2 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 4 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 5 | 5 | 1 | 7 | 16 | 18 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 9 | 11 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 55 | 27 | 14 | 8 | 45 | 46 | 48 | 44 | 38 | 36 | 19 | 28 | 143 | 205 | 207 | 121 | 103 | 74 | 75 | 30 | 28 | 109 | 139 | 78 |
Endoplasmic Reticulum | 2 | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 2 | 0 | 1 | 1 | 5 | 23 | 12 | 12 | 1 | 0 | 0 | 1 | 1 | 5 |
Endosome | 2 | 0 | 0 | 0 | 5 | 1 | 4 | 0 | 2 | 1 | 0 | 0 | 9 | 20 | 30 | 15 | 20 | 28 | 5 | 2 | 1 | 7 | 11 | 11 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 2 | 3 | 1 | 0 | 0 | 0 | 0 | 1 |
Mitochondria | 76 | 5 | 5 | 5 | 19 | 42 | 65 | 84 | 105 | 91 | 61 | 89 | 8 | 5 | 2 | 14 | 6 | 11 | 7 | 1 | 6 | 9 | 16 | 11 |
Nucleus | 48 | 21 | 11 | 3 | 7 | 15 | 17 | 22 | 16 | 23 | 7 | 22 | 56 | 57 | 71 | 58 | 46 | 32 | 104 | 55 | 32 | 86 | 132 | 83 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 2 | 2 |
Nucleolus | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 4 | 2 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 7 | 3 | 4 | 13 | 25 | 17 |
Vac/Vac Membrane | 90 | 38 | 24 | 2 | 36 | 10 | 24 | 24 | 29 | 17 | 8 | 24 | 174 | 282 | 310 | 118 | 129 | 135 | 45 | 35 | 26 | 63 | 91 | 119 |
Unique Cell Count | 221 | 78 | 49 | 16 | 98 | 94 | 127 | 145 | 152 | 132 | 82 | 125 | 359 | 511 | 548 | 308 | 270 | 245 | 266 | 138 | 106 | 312 | 468 | 375 |
Labelled Cell Count | 275 | 91 | 55 | 18 | 113 | 114 | 160 | 182 | 198 | 172 | 99 | 166 | 392 | 570 | 626 | 352 | 321 | 297 | 266 | 138 | 106 | 312 | 468 | 375 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.2 | 5.0 | 5.0 | 4.9 | 4.8 | 4.4 | 4.0 | 4.4 | 4.3 | 3.8 | 4.0 | 4.1 | 5.4 | 5.8 | 5.8 | 6.0 | 7.3 | 7.2 | 4.8 | 4.9 | 5.8 |
Std Deviation (1e-4) | 0.7 | 0.7 | 1.3 | 1.5 | 1.2 | 1.2 | 1.2 | 1.5 | 1.5 | 0.9 | 0.8 | 0.8 | 0.9 | 1.0 | 1.0 | 1.1 | 1.6 | 1.7 | 1.0 | 1.0 | 1.2 |
Intensity Change (Log2) | – | – | – | -0.01 | -0.04 | -0.16 | -0.31 | -0.17 | -0.19 | -0.4 | -0.32 | -0.29 | 0.13 | 0.23 | 0.22 | 0.28 | 0.55 | 0.53 | -0.06 | -0.01 | 0.23 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1.6 | 2.0 | 2.3 | 1.1 | 0.2 | -0.5 | -0.2 | -0.7 | -0.9 | 1.5 | 1.6 | 1.3 | 1.4 | 1.3 | 0.2 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | -2.7 | -1.0 | -1.5 | -1.2 | -2.1 | -0.8 | -2.2 | -0.7 | -1.2 | -2.3 | -1.8 | -0.6 | -0.9 | -1.7 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -2.6 | -1.4 | -5.1 | -4.0 | -4.5 | -4.1 | -5.2 | -5.1 | -4.0 | -0.1 | 0.8 | 1.0 | -1.4 | -0.2 | 0.8 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.3517 | 2.0804 | 2.1664 | 2.3426 | 1.5758 | 2.2085 | 2.6041 | 2.4115 | 2.5917 | 1.5689 | 2.7527 | 2.4668 | 3.4646 | 3.5158 | 3.3914 | 4.099 | 2.598 | 3.2893 |
Actin | 0.0471 | 0.0017 | 0.0661 | 0.0035 | 0.0003 | 0.0065 | 0.039 | 0.0007 | 0.0119 | 0.0004 | 0.033 | 0.0073 | 0.0428 | 0.0001 | 0.0311 | 0.0008 | 0.0696 | 0.0107 |
Bud | 0.0003 | 0.0005 | 0.0008 | 0.0006 | 0.0001 | 0.0011 | 0.0023 | 0.0004 | 0.011 | 0.0001 | 0.0009 | 0.0027 | 0.0011 | 0.0017 | 0.0002 | 0.0005 | 0.0007 | 0.0017 |
Bud Neck | 0.0002 | 0.0007 | 0.0013 | 0.0005 | 0.0001 | 0.003 | 0.0026 | 0.0003 | 0.0015 | 0.0426 | 0.0078 | 0.0119 | 0.0064 | 0.0001 | 0.0007 | 0.0002 | 0.0015 | 0.0032 |
Bud Periphery | 0.0005 | 0.0005 | 0.001 | 0.0009 | 0 | 0.0027 | 0.006 | 0.0003 | 0.0043 | 0.0001 | 0.0011 | 0.0023 | 0.0014 | 0.0002 | 0.0002 | 0.0006 | 0.0013 | 0.0043 |
Bud Site | 0.0009 | 0.0103 | 0.0036 | 0.0003 | 0.0001 | 0.0003 | 0.0064 | 0.0013 | 0.0082 | 0.0005 | 0.0133 | 0.0026 | 0.0192 | 0.0023 | 0.0011 | 0.0009 | 0.0041 | 0.0011 |
Cell Periphery | 0.0001 | 0.0004 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0006 | 0.0001 | 0.0002 | 0 | 0.0004 | 0.0001 | 0.0005 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0002 |
Cytoplasm | 0.1047 | 0.2909 | 0.4086 | 0.2521 | 0.378 | 0.4103 | 0.2763 | 0.4078 | 0.2963 | 0.3828 | 0.3591 | 0.3818 | 0.2336 | 0.3618 | 0.2994 | 0.2181 | 0.4474 | 0.36 |
Cytoplasmic Foci | 0.0159 | 0.0156 | 0.0159 | 0.0045 | 0.007 | 0.0158 | 0.0208 | 0.0104 | 0.0226 | 0.0041 | 0.0171 | 0.0151 | 0.0264 | 0.006 | 0.0085 | 0.0186 | 0.0121 | 0.0155 |
Eisosomes | 0.0005 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0.0003 | 0 | 0.0003 | 0.0001 | 0.0013 | 0.0001 | 0.0002 | 0.0001 | 0.0007 | 0.0001 |
Endoplasmic Reticulum | 0.0035 | 0.0034 | 0.007 | 0.0039 | 0.0033 | 0.0046 | 0.0041 | 0.0042 | 0.005 | 0.0021 | 0.0034 | 0.0041 | 0.0057 | 0.0027 | 0.0069 | 0.0023 | 0.004 | 0.0034 |
Endosome | 0.0148 | 0.0044 | 0.0056 | 0.0023 | 0.0045 | 0.0061 | 0.0068 | 0.0058 | 0.011 | 0.002 | 0.0139 | 0.0091 | 0.0234 | 0.0024 | 0.0063 | 0.0131 | 0.0106 | 0.0052 |
Golgi | 0.0089 | 0.0011 | 0.0018 | 0.0002 | 0.0001 | 0.0007 | 0.0019 | 0.0002 | 0.0022 | 0.0001 | 0.0054 | 0.0017 | 0.016 | 0.0001 | 0.002 | 0.0009 | 0.0062 | 0.0022 |
Lipid Particles | 0.0398 | 0.0014 | 0.0017 | 0.0002 | 0.0002 | 0.0008 | 0.0088 | 0.0004 | 0.0022 | 0 | 0.0052 | 0.0014 | 0.0159 | 0.0001 | 0.0019 | 0.0082 | 0.0129 | 0.0071 |
Mitochondria | 0.0024 | 0.0006 | 0.0011 | 0.0017 | 0.0005 | 0.0027 | 0.0016 | 0.0005 | 0.0011 | 0.0005 | 0.0022 | 0.0014 | 0.0133 | 0.0003 | 0.0007 | 0.0008 | 0.0057 | 0.0035 |
None | 0.6987 | 0.6087 | 0.3356 | 0.6724 | 0.5691 | 0.412 | 0.4778 | 0.476 | 0.4856 | 0.4106 | 0.4302 | 0.3905 | 0.5491 | 0.526 | 0.5813 | 0.6765 | 0.266 | 0.4734 |
Nuclear Periphery | 0.0166 | 0.0024 | 0.0096 | 0.0048 | 0.003 | 0.0065 | 0.0124 | 0.0069 | 0.0077 | 0.0038 | 0.0063 | 0.0081 | 0.0024 | 0.0028 | 0.0051 | 0.0016 | 0.0061 | 0.004 |
Nucleolus | 0.0006 | 0.0023 | 0.001 | 0.0006 | 0.0002 | 0.0021 | 0.0041 | 0.0006 | 0.0018 | 0.0006 | 0.0015 | 0.0015 | 0.0012 | 0.0007 | 0.0005 | 0.0002 | 0.0007 | 0.0008 |
Nucleus | 0.0009 | 0.0453 | 0.1217 | 0.046 | 0.0302 | 0.1026 | 0.0889 | 0.0689 | 0.0764 | 0.1399 | 0.0757 | 0.1133 | 0.0199 | 0.0753 | 0.04 | 0.0164 | 0.1286 | 0.0833 |
Peroxisomes | 0.022 | 0.0005 | 0.0058 | 0.0005 | 0.0001 | 0.0025 | 0.0106 | 0.0004 | 0.0085 | 0.0001 | 0.0023 | 0.0016 | 0.0152 | 0.0001 | 0.0024 | 0.0363 | 0.0092 | 0.0033 |
Punctate Nuclear | 0.02 | 0.0063 | 0.0082 | 0.0029 | 0.0021 | 0.0166 | 0.0252 | 0.0124 | 0.0404 | 0.0088 | 0.0181 | 0.0415 | 0.0022 | 0.0153 | 0.0097 | 0.0029 | 0.01 | 0.0155 |
Vacuole | 0.0005 | 0.0026 | 0.0028 | 0.0014 | 0.0009 | 0.0027 | 0.0028 | 0.0021 | 0.0015 | 0.0008 | 0.0022 | 0.0015 | 0.0027 | 0.0015 | 0.0013 | 0.0008 | 0.0017 | 0.0012 |
Vacuole Periphery | 0.0012 | 0.0002 | 0.0004 | 0.0004 | 0.0002 | 0.0003 | 0.0006 | 0.0003 | 0.0003 | 0.0002 | 0.0006 | 0.0004 | 0.0005 | 0.0002 | 0.0002 | 0.0002 | 0.0005 | 0.0003 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 2.8457 | 2.5438 | 2.3596 | 5.1131 | 3.6895 | 4.9771 | 5.1442 | 4.7739 | 6.3432 | 4.9926 |
Translational Efficiency | – | – | – | – | – | – | – | – | – | – |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | – |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1836 | 1592 | 220 | 1369 | 2346 | 1482 | 198 | 464 | 4182 | 3074 | 418 | 1833 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 684.58 | 787.46 | 936.40 | 904.00 | 714.89 | 765.98 | 1059.97 | 825.47 | 701.58 | 777.10 | 994.93 | 884.12 |
Standard Deviation | 85.82 | 107.93 | 111.85 | 135.79 | 78.94 | 99.95 | 132.71 | 124.21 | 83.40 | 104.71 | 136.87 | 137.27 |
Intensity Change Log 2 | — | 0.201987 | 0.451906 | 0.401104 | — | 0.099585 | 0.568230 | 0.207495 | — | 0.150586 | 0.512499 | 0.305449 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000022 | 0.000358 | 0.001115 | 0.001704 | 0.000025 | 0.000454 | 0.001676 | 0.000677 | 0.000023 | 0.000404 | 0.001381 | 0.001444 |
Bud Neck | 0.003452 | 0.008295 | 0.006527 | 0.011456 | 0.002908 | 0.010126 | 0.005827 | 0.004234 | 0.003147 | 0.009178 | 0.006195 | 0.009628 |
Bud Site | 0.000572 | 0.007754 | 0.007287 | 0.014695 | 0.000499 | 0.007563 | 0.005429 | 0.008496 | 0.000531 | 0.007662 | 0.006407 | 0.013126 |
Cell Periphery | 0.000380 | 0.000114 | 0.000166 | 0.000172 | 0.000096 | 0.000108 | 0.000187 | 0.000231 | 0.000221 | 0.000111 | 0.000176 | 0.000187 |
Cytoplasm | 0.240758* | 0.305461* | 0.039066 | 0.189961* | 0.250452* | 0.300646* | 0.039374 | 0.262546* | 0.246196* | 0.303140* | 0.039212 | 0.208335* |
Cytoplasmic Foci | 0.015833 | 0.038909 | 0.000394 | 0.004183 | 0.025235 | 0.032034 | 0.000792 | 0.023517 | 0.021108 | 0.035595 | 0.000583 | 0.009077 |
Eisosomes | 0.000079 | 0.000036 | 0.000036 | 0.000030 | 0.000026 | 0.000032 | 0.000077 | 0.000059 | 0.000049 | 0.000034 | 0.000055 | 0.000037 |
Endoplasmic Reticulum | 0.000610 | 0.002434 | 0.011303 | 0.006087 | 0.000758 | 0.001853 | 0.021680 | 0.003869 | 0.000693 | 0.002154 | 0.016219 | 0.005525 |
Endosome | 0.000757 | 0.005000 | 0.000931 | 0.005530 | 0.000996 | 0.004906 | 0.001685 | 0.012459 | 0.000891 | 0.004955 | 0.001289 | 0.007284 |
Golgi | 0.000233 | 0.002278 | 0.000091 | 0.003828 | 0.000154 | 0.002236 | 0.000105 | 0.006844 | 0.000189 | 0.002258 | 0.000098 | 0.004591 |
Lipid Particles | 0.002054 | 0.003115 | 0.000633 | 0.002591 | 0.002752 | 0.002642 | 0.001479 | 0.003476 | 0.002446 | 0.002887 | 0.001034 | 0.002815 |
Mitochondria | 0.000675 | 0.007056 | 0.000936 | 0.002983 | 0.001219 | 0.006827 | 0.001142 | 0.008725 | 0.000980 | 0.006946 | 0.001034 | 0.004436 |
Mitotic Spindle | 0.000326 | 0.021923 | 0.000951 | 0.043292 | 0.001597 | 0.014454 | 0.000708 | 0.014492 | 0.001039 | 0.018322 | 0.000836 | 0.036002 |
None | 0.019134 | 0.009234 | 0.004707 | 0.009643 | 0.006464 | 0.007237 | 0.003797 | 0.026470 | 0.012026 | 0.008271 | 0.004276 | 0.013903 |
Nuclear Periphery | 0.000992 | 0.002105 | 0.001493 | 0.004680 | 0.001981 | 0.002621 | 0.003207 | 0.001584 | 0.001547 | 0.002354 | 0.002305 | 0.003896 |
Nuclear Periphery Foci | 0.000299 | 0.001446 | 0.001542 | 0.001650 | 0.000422 | 0.000799 | 0.001833 | 0.002294 | 0.000368 | 0.001134 | 0.001680 | 0.001813 |
Nucleolus | 0.002117 | 0.004030 | 0.001377 | 0.001488 | 0.001877 | 0.002996 | 0.000904 | 0.003678 | 0.001982 | 0.003531 | 0.001153 | 0.002043 |
Nucleus | 0.707991* | 0.556409* | 0.907133* | 0.660292* | 0.697341* | 0.583909* | 0.852643* | 0.563451* | 0.702017* | 0.569667* | 0.881322* | 0.635778* |
Peroxisomes | 0.000362 | 0.003988 | 0.000183 | 0.001115 | 0.000731 | 0.003941 | 0.000306 | 0.003804 | 0.000569 | 0.003965 | 0.000241 | 0.001796 |
Vacuole | 0.003127 | 0.018772 | 0.013985 | 0.032247 | 0.004305 | 0.013619 | 0.056952 | 0.045549 | 0.003788 | 0.016288 | 0.034338 | 0.035614 |
Vacuole Periphery | 0.000227 | 0.001282 | 0.000144 | 0.002373 | 0.000163 | 0.000996 | 0.000197 | 0.003545 | 0.000191 | 0.001144 | 0.000169 | 0.002670 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -6.86 | -8.64 | -4.52 | -2.90 | 0.96 | -2.95 | -11.51 | -9.62 | -2.68 | 5.74 | -5.23 | -14.14 | -5.76 | -3.24 | 3.07 |
Bud Neck | -4.49 | -4.39 | -7.76 | -3.69 | -3.70 | -5.79 | -4.93 | -3.60 | 3.58 | 2.20 | -7.55 | -6.82 | -9.80 | -1.62 | -3.05 |
Bud Site | -5.94 | -1.68 | -6.17 | -0.20 | -0.66 | -5.64 | -3.46 | -5.14 | -0.10 | -0.52 | -8.20 | -3.04 | -7.65 | -0.30 | -0.87 |
Cell Periphery | 4.79 | 3.26 | 4.56 | -1.17 | 1.83 | -1.03 | -6.58 | -5.98 | -4.84 | 0.82 | 4.28 | 0.76 | 2.88 | -3.41 | 2.83 |
Cytoplasm | -6.74 | 27.50 | 16.34 | 22.12 | -12.85 | -5.53 | 32.47 | 3.44 | 6.77 | -14.49 | -8.64 | 42.28 | 17.44 | 23.58 | -19.29 |
Cytoplasmic Foci | -9.48 | 15.36 | 13.29 | 16.68 | -5.97 | -2.94 | 16.98 | 5.40 | 7.32 | -5.93 | -8.33 | 22.26 | 15.50 | 19.80 | -7.42 |
Eisosomes | 2.02 | 1.62 | 1.79 | -1.80 | 0.54 | -3.79 | -9.89 | -11.82 | -9.49 | 2.22 | 1.42 | -2.31 | -0.39 | -7.31 | 3.96 |
Endoplasmic Reticulum | -12.15 | -10.34 | -20.73 | -14.50 | 3.85 | -7.50 | -14.67 | -12.14 | -9.75 | 9.93 | -13.88 | -17.32 | -23.74 | -17.99 | 9.47 |
Endosome | -7.32 | -2.04 | -4.30 | 3.94 | -2.96 | -5.62 | -4.92 | -4.30 | -1.22 | -3.31 | -9.05 | -4.73 | -5.90 | 2.42 | -3.92 |
Golgi | -3.95 | 0.71 | -1.51 | 1.56 | -1.79 | -3.23 | 0.17 | -2.47 | -0.57 | -2.48 | -5.07 | 0.77 | -2.71 | 1.04 | -2.86 |
Lipid Particles | -1.98 | 2.60 | 1.11 | 3.56 | -1.41 | 0.19 | 2.96 | 0.19 | 0.04 | -1.81 | -1.34 | 4.23 | 1.83 | 3.11 | -1.40 |
Mitochondria | -5.72 | -2.75 | -7.80 | 3.89 | -6.55 | -4.60 | -0.58 | -1.34 | 0.99 | -1.29 | -7.23 | -1.79 | -3.41 | 3.83 | -3.05 |
Mitotic Spindle | -7.81 | -1.34 | -9.48 | -2.91 | -7.77 | -5.33 | -1.34 | -4.24 | -1.62 | -3.66 | -9.31 | -1.78 | -10.19 | -3.92 | -8.76 |
None | 5.54 | 8.65 | 6.03 | 0.36 | -5.72 | -0.62 | 4.87 | -2.89 | -2.73 | -4.12 | 4.23 | 9.71 | 1.39 | -2.28 | -7.18 |
Nuclear Periphery | -7.22 | -5.24 | -12.33 | -9.38 | -8.95 | -1.03 | -5.70 | -3.21 | -0.21 | 3.74 | -2.79 | -6.97 | -11.55 | -7.42 | -6.75 |
Nuclear Periphery Foci | -3.87 | -1.84 | -7.83 | -0.20 | 0.07 | -2.56 | -3.94 | -3.36 | -2.44 | 0.57 | -4.53 | -3.74 | -7.38 | -2.13 | 0.76 |
Nucleolus | -3.79 | 1.89 | 2.21 | 5.40 | 0.11 | -2.45 | 3.90 | -1.14 | 0.29 | -2.36 | -4.50 | 3.75 | 0.69 | 4.53 | -2.13 |
Nucleus | 14.64 | -16.42 | -0.90 | -14.51 | 14.83 | 11.50 | -5.26 | 7.10 | 0.02 | 9.22 | 18.55 | -13.67 | 2.30 | -12.60 | 13.71 |
Peroxisomes | -5.09 | 2.69 | -0.00 | 5.06 | -1.63 | -4.98 | 3.79 | -1.22 | 2.38 | -1.87 | -7.04 | 4.69 | -0.53 | 6.29 | -2.25 |
Vacuole | -12.49 | -5.17 | -16.97 | -11.12 | -7.60 | -8.80 | -8.71 | -11.90 | -10.43 | 0.56 | -15.20 | -9.41 | -20.53 | -15.64 | -1.30 |
Vacuole Periphery | -3.59 | 0.52 | -2.01 | 1.47 | -2.50 | -3.80 | -1.69 | -2.43 | -1.00 | -2.35 | -5.21 | 0.04 | -3.29 | 0.56 | -3.38 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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