Standard name
Human Ortholog
Description Phosphoinositide PI4,5P(2) binding protein, forms a complex with Slm2p; acts downstream of Mss4p in a pathway regulating actin cytoskeleton organization in response to stress; TORC2 complex substrate and effector; protein abundance increases in response to DNA replication stress; SLM1 has a paralog, SLM2, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.7 0.64 0.59 0.68 0.75 0.61 0.64 0.59 0.48 0.51 0.42 0.37 0.66 0.66 0.53 0.62 0.55 0.58 0.32 0.43 0.3 0.35 0.35 0.32
Bud 0.17 0.14 0.05 0.1 0.17 0.14 0.17 0.15 0.16 0.12 0.14 0.16 0.12 0.11 0.05 0.11 0.1 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.41 0.36 0.47 0.38 0.25 0.2 0.24 0.22 0.27 0.24 0.19 0.21 0.45 0.39 0.42 0.42 0.45 0.3 0.56 0.37 0.57 0.48 0.51 0.51
Cytoplasm 0.1 0.12 0.16 0.09 0.1 0.06 0.09 0.11 0.1 0.13 0.07 0.14 0.08 0.07 0.08 0.09 0.08 0.19 0 0 0 0 0 0
Endoplasmic Reticulum 0.06 0.12 0.08 0 0.05 0.09 0.08 0.11 0.11 0.12 0.05 0 0 0 0.05 0 0.06 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0.05 0.08 0.17 0.18 0.09 0 0 0 0 0 0
Mitochondria 0.1 0.07 0 0.17 0.05 0.3 0.25 0.23 0.43 0.37 0.64 0.5 0 0 0 0.08 0.1 0.14 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0.13 0.08 0.29 0.15 0.08 0.18 0.06 0.13 0.16 0.1 0.24 0.29 0.11 0.07 0.11 0 0 0 0 0 0
SpindlePole 0 0 0 0.13 0.07 0.08 0.09 0.09 0 0.05 0 0 0.1 0.14 0.09 0.06 0 0.09 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 252 226 127 88 158 109 169 192 101 104 81 67 291 303 271 41 46 33 134 190 76 95 164 150
Bud 61 50 11 13 36 25 44 50 33 24 28 30 52 50 28 7 8 0 0 0 0 1 0 0
Bud Neck 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 10 16 11 10 17 22
Bud Site 1 1 0 3 7 5 1 4 0 0 2 1 2 2 0 1 0 0 – – – – – –
Cell Periphery 147 126 101 49 53 35 64 72 56 49 37 38 196 176 213 28 37 17 232 165 146 131 242 244
Cytoplasm 36 44 35 12 22 11 24 37 21 27 13 25 36 32 42 6 7 11 1 5 1 3 5 6
Endoplasmic Reticulum 23 44 18 5 10 16 22 37 22 25 10 7 11 6 25 2 5 2 5 14 1 9 11 12
Endosome 2 3 0 0 0 0 2 3 4 3 0 2 1 0 3 0 1 0 6 9 3 3 5 6
Golgi 1 10 3 3 11 3 2 4 1 2 0 1 5 24 40 11 15 5 3 9 1 0 2 2
Mitochondria 36 25 1 22 11 53 65 73 90 76 125 91 14 12 9 5 8 8 13 17 10 11 10 13
Nucleus 0 2 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 1 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 10 14 6 17 17 52 40 25 37 13 26 30 46 111 148 7 6 6 3 2 3 1 6 9
SpindlePole 14 4 9 17 14 14 23 29 8 10 3 5 43 66 46 4 2 5 0 0 0 0 0 1
Vac/Vac Membrane 0 1 1 0 0 0 0 0 0 0 0 0 1 0 0 0 2 3 1 3 0 2 2 2
Unique Cell Count 362 352 215 129 211 178 263 324 209 203 195 183 439 457 510 66 83 57 416 442 255 275 472 478
Labelled Cell Count 583 550 312 230 339 323 456 530 374 333 326 299 698 782 825 112 137 90 416 442 255 275 472 478


Punctate Nuclear

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.5 8.6 8.7 7.8 8.2 7.3 7.1 7.6 6.6 6.9 6.3 6.7 9.2 9.0 9.1 10.1 10.0 9.0 8.3 8.7 8.8
Std Deviation (1e-4) 0.8 1.3 1.3 1.5 1.5 1.3 1.0 1.3 1.2 1.4 1.0 1.5 1.2 1.1 1.3 1.4 1.6 1.8 1.2 1.4 1.4
Intensity Change (Log2) – – – -0.17 -0.09 -0.26 -0.29 -0.19 -0.41 -0.35 -0.48 -0.39 0.08 0.04 0.06 0.21 0.2 0.05 -0.06 -0.01 0.01


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 1.7 3.5 0.4 1.2 0 -2.2 -1.6 -3.5 -4.5 1.8 1.8 -1.5 0.4 -0.6 -0.2
Bud 1.7 3.9 3.1 4.0 3.7 3.6 2.5 3.2 3.7 2.7 2.5 0.2 0 1.4 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -1.6 -4.7 -5.7 -5.2 -6.0 -4.3 -4.9 -6.0 -5.5 -0.6 -2.1 -1.3 -0.6 -0.4 -2.3
Cytoplasm -1.8 -1.8 -3.1 -2.4 -1.6 -1.9 -0.9 -3.0 -0.7 -3.1 -3.7 -3.2 -1.4 -1.7 0.5
Endoplasmic Reticulum -1.6 -1.5 0.2 0 1.1 0.8 1.3 -1.3 -1.9 -3.4 -4.6 -1.8 0 -0.7 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 2.2 0 0 0 0 0 0 0 0 2.4 3.4 0 5.4 0
Mitochondria 6.0 3.0 8.4 7.6 7.3 10.7 9.7 13.9 11.6 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 3.7 2.4 7.4 4.6 2.4 5.1 1.8 4.0 4.7 3.4 6.9 7.9 0 0 0
SpindlePole 3.1 1.1 1.5 2.0 2.1 -0.2 0.4 -1.6 -0.8 2.5 3.9 2.2 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.9062 5.3471 4.6474 4.7593 3.7865 4.6023 3.9435 4.5331 3.9738 3.7571 3.9974 4.1432 4.9184 6.2187 5.6559 5.9079 5.2903 5.6054
Actin 0.1549 0.2764 0.2568 0.2124 0.2019 0.1878 0.2741 0.2515 0.2643 0.2407 0.1953 0.1634 0.1771 0.2558 0.2004 0.1818 0.3714 0.2026
Bud 0.0057 0.0046 0.0167 0.0422 0.0055 0.0106 0.0021 0.0064 0.0027 0.001 0.0048 0.0043 0.0005 0.0006 0.0006 0.0009 0.0007 0.0009
Bud Neck 0.0171 0.0167 0.0203 0.0339 0.0217 0.0772 0.0146 0.022 0.0331 0.0582 0.0109 0.0568 0.019 0.0177 0.0441 0.0557 0.0246 0.0372
Bud Periphery 0.0133 0.0041 0.0164 0.0446 0.0356 0.0411 0.0096 0.0054 0.0081 0.0077 0.0127 0.0126 0.0037 0.0024 0.0035 0.009 0.0053 0.0059
Bud Site 0.0878 0.2367 0.127 0.0551 0.0059 0.0204 0.0383 0.2115 0.1224 0.0248 0.0405 0.0087 0.0267 0.1145 0.0582 0.0132 0.0073 0.0043
Cell Periphery 0.1972 0.1277 0.1545 0.178 0.217 0.2563 0.1806 0.1467 0.1776 0.2872 0.2624 0.3414 0.1754 0.1712 0.2454 0.1615 0.1628 0.2511
Cytoplasm 0.0779 0.1389 0.0758 0.0941 0.1789 0.1134 0.0472 0.0763 0.0743 0.0585 0.0511 0.0867 0.0613 0.0832 0.0596 0.0692 0.0218 0.075
Cytoplasmic Foci 0.0302 0.0089 0.0126 0.0247 0.0138 0.0088 0.024 0.0038 0.01 0.0047 0.0191 0.0071 0.0022 0.0036 0.0029 0.0031 0.0047 0.0076
Eisosomes 0.2718 0.1572 0.2388 0.2494 0.1571 0.2233 0.3082 0.2618 0.2519 0.2343 0.2725 0.2872 0.5142 0.3254 0.369 0.4782 0.371 0.3926
Endoplasmic Reticulum 0.0061 0.0041 0.0069 0.0066 0.0062 0.0072 0.0035 0.0017 0.0026 0.0051 0.0028 0.0025 0.0088 0.0072 0.0048 0.0046 0.0045 0.0068
Endosome 0.0169 0.0014 0.0159 0.0096 0.0326 0.0013 0.0214 0.0006 0.0114 0.0072 0.0237 0.0015 0.0004 0.0006 0.0003 0.0005 0.0005 0.0006
Golgi 0.0175 0.0109 0.0277 0.0133 0.0463 0.0136 0.0187 0.0064 0.0108 0.0107 0.0374 0.0067 0.0049 0.0094 0.0054 0.0152 0.0069 0.0089
Lipid Particles 0.0128 0.0059 0.0049 0.0046 0.0074 0.0047 0.0187 0.0012 0.0094 0.0091 0.0089 0.0021 0.0022 0.0053 0.0021 0.0028 0.0017 0.0024
Mitochondria 0.03 0.001 0.003 0.0037 0.0495 0.0065 0.0118 0.0006 0.0037 0.0427 0.0184 0.0048 0.0005 0.0004 0.0004 0.0009 0.0132 0.0006
None 0.0317 0.0028 0.0072 0.0144 0.0016 0.013 0.0053 0.0022 0.0051 0.002 0.0113 0.0071 0.0016 0.0014 0.0016 0.0013 0.0007 0.0016
Nuclear Periphery 0.003 0.0002 0.0037 0.0012 0.002 0.0008 0.0034 0.0002 0.0009 0.0003 0.0004 0.0002 0.0003 0.0003 0.0003 0.0002 0.0003 0.0003
Nucleolus 0.0034 0.0002 0.0009 0.001 0.0002 0.0013 0.0011 0.0002 0.0013 0.0002 0.0006 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001
Nucleus 0.0042 0.0003 0.0012 0.0017 0.0004 0.0026 0.0013 0.0003 0.0018 0.0002 0.0007 0.0002 0.0001 0.0001 0.0001 0.0002 0.0001 0.0001
Peroxisomes 0.0069 0.001 0.0027 0.0027 0.0067 0.0031 0.0095 0.0006 0.0038 0.0035 0.0229 0.0055 0.0005 0.0004 0.0005 0.0004 0.0022 0.0006
Punctate Nuclear 0.0042 0.0002 0.0007 0.003 0.0002 0.0015 0.0037 0.0001 0.0017 0.0002 0.0008 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001
Vacuole 0.0058 0.0008 0.0037 0.0034 0.0054 0.0051 0.002 0.0005 0.0027 0.0009 0.0021 0.0008 0.0005 0.0006 0.0005 0.0007 0.0003 0.0006
Vacuole Periphery 0.0016 0.0001 0.0025 0.0005 0.0039 0.0003 0.0008 0 0.0004 0.0008 0.0005 0.0001 0 0 0 0.0001 0.0001 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 11.2428 16.2721 11.3643 17.2914 11.4532 15.5172 18.3883 19.2695 17.0484 18.6149
Translational Efficiency 1.0479 0.9122 1.1079 0.5941 1.1319 1.0505 0.9899 0.7472 0.9863 0.8582

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Phosphoinositide PI4,5P(2) binding protein, forms a complex with Slm2p; acts downstream of Mss4p in a pathway regulating actin cytoskeleton organization in response to stress; TORC2 complex substrate and effector; protein abundance increases in response to DNA replication stress; SLM1 has a paralog, SLM2, that arose from the whole genome duplication
Localization
Cell Percentages actin (17%), cell periphery (12%), mixed (38%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Slm1

Slm1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Slm1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available