ORF
Human Ortholog
Description Putative metalloendopeptidase; forms cytoplasmic foci upon DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.06 0.07 0.06 0 0 0 0 0 0
Cytoplasm 0.89 0.97 0.97 0.97 0.94 0.9 0.92 0.89 0.82 – 0.71 0.82 0.99 0.99 1.0 0.92 0.86 0.83 0.84 0.86 0.89 0.9 0.85 0.81
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.06 0 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.11 0.07 0.05 0.22 – 0.32 0.11 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.05 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.05 0 – 0 0.06 0 0 0 0.05 0.06 0.07 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 9 0 2 1 9 1 3 1 0 – 0 3 1 0 0 0 1 1 4 3 0 0 3 5
Bud 3 4 3 5 7 8 5 2 5 – 10 10 1 2 0 0 1 2 5 7 0 1 8 8
Bud Neck 0 0 0 0 3 1 1 1 0 – 0 0 0 0 0 0 0 0 2 2 0 0 2 3
Bud Site 2 0 0 0 1 0 2 0 0 – 0 1 0 0 0 0 0 1 – – – – – –
Cell Periphery 0 2 4 4 7 8 8 9 5 – 14 14 3 4 0 16 14 11 0 0 0 0 2 3
Cytoplasm 226 254 276 311 412 404 612 413 340 – 253 383 406 622 683 241 173 156 223 230 268 163 387 378
Endoplasmic Reticulum 0 1 0 0 6 0 0 5 3 – 2 11 0 1 1 15 6 11 0 0 0 2 11 5
Endosome 4 2 2 3 1 6 3 3 0 – 0 0 0 1 1 6 6 4 4 5 4 0 6 14
Golgi 0 0 0 0 0 1 0 0 0 – 0 0 0 0 1 1 2 3 4 0 0 0 0 1
Mitochondria 4 1 0 10 6 47 50 22 92 – 114 51 2 0 1 0 1 4 0 1 4 5 14 19
Nucleus 0 1 1 2 0 3 5 8 4 – 4 5 1 0 0 5 6 4 0 0 0 0 0 0
Nuclear Periphery 0 1 0 0 0 1 0 0 0 – 1 0 2 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 1 1 0 0 0 3 0 0 0 – 1 0 1 0 0 1 1 0 0 1 0 0 0 0
Peroxisomes 2 1 2 0 0 0 0 0 0 – 0 0 0 1 0 0 1 0 0 0 0 0 0 1
SpindlePole 12 1 2 2 9 8 4 4 0 – 1 1 0 1 0 2 2 3 15 4 4 0 1 6
Vac/Vac Membrane 3 1 0 2 3 14 13 22 15 – 15 28 0 4 0 12 13 14 0 8 11 3 11 15
Unique Cell Count 255 263 284 322 439 447 667 465 415 355 465 411 629 686 261 202 187 266 269 302 182 457 469
Labelled Cell Count 266 270 292 340 464 505 706 490 464 415 507 417 636 687 299 227 214 266 269 302 182 457 469


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.8 5.8 6.4 5.6 6.3 5.4 5.6 6.0 5.0 – 4.8 5.6 6.2 6.2 6.3 10.4 10.6 9.7 5.3 5.6 5.7
Std Deviation (1e-4) 1.1 1.3 1.1 0.9 1.3 1.2 1.2 1.0 1.0 – 0.9 1.0 1.3 1.2 1.3 2.9 2.8 2.5 1.2 1.1 1.3
Intensity Change (Log2) – – – -0.2 -0.03 -0.23 -0.2 -0.09 -0.37 – -0.42 -0.2 -0.05 -0.05 -0.01 0.7 0.72 0.6 -0.27 -0.2 -0.16


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 – 0 1.4 0 0 0 2.9 3.2 2.7
Cytoplasm -0.4 -2.0 -3.5 -3.1 -4.1 -6.1 – -8.6 -6.1 1.5 1.9 3.2 -2.6 -4.7 -5.3
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 4.1 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0 0 5.6 4.7 3.7 8.5 – 0 5.8 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vacuole 0 0 3.0 0 3.7 3.2 – 3.5 4.2 0 0 0 3.7 4.3 4.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.9631 3.3805 2.3796 2.4351 3.1098 3.1215 3.2853 4.64 4.018 3.7805 3.2153 4.0204 3.1504 5.5095 4.1383 4.6438 2.797 4.2193
Actin 0.0415 0.0035 0.0243 0.0994 0.0024 0.0208 0.0384 0.0032 0.0104 0.008 0.0103 0.0098 0.0518 0.0087 0.0155 0.0086 0.0039 0.0067
Bud 0.0006 0.0012 0.0005 0.0007 0.0004 0.0004 0.0029 0.002 0.0074 0.0009 0.0011 0.0041 0.002 0.0027 0.0069 0.0022 0.0001 0.0005
Bud Neck 0.0015 0.0004 0.0007 0.0084 0.0379 0.0528 0.0054 0.0007 0.0026 0.0017 0.0013 0.0093 0.0091 0.0011 0.0057 0.007 0.0006 0.0415
Bud Periphery 0.0008 0.0004 0.0005 0.0005 0.0002 0.0006 0.006 0.0007 0.0043 0.0005 0.0011 0.005 0.0015 0.0015 0.0048 0.0013 0.0001 0.0003
Bud Site 0.0056 0.0075 0.0046 0.0019 0.0189 0.0014 0.0139 0.0323 0.0094 0.0011 0.002 0.001 0.0103 0.0418 0.0124 0.0036 0.0003 0.001
Cell Periphery 0.0006 0.0005 0.0004 0.0002 0.0003 0.0008 0.0006 0.0002 0.0002 0.0002 0.0002 0.0001 0.0004 0.0002 0.0004 0.0004 0 0.0001
Cytoplasm 0.5938 0.8738 0.7388 0.7109 0.5631 0.7268 0.6465 0.8342 0.7615 0.7752 0.7483 0.8002 0.6112 0.7011 0.6474 0.5599 0.6888 0.7072
Cytoplasmic Foci 0.1303 0.0504 0.1052 0.058 0.2876 0.0901 0.1407 0.0812 0.1025 0.1052 0.0734 0.0945 0.1599 0.1712 0.2035 0.2829 0.2006 0.1968
Eisosomes 0.0006 0 0.0001 0.0002 0.0001 0.0001 0.0002 0 0 0 0.0001 0.0002 0.0005 0 0 0 0 0
Endoplasmic Reticulum 0.0131 0.0028 0.0058 0.0057 0.0056 0.0027 0.0064 0.0015 0.0024 0.0046 0.0027 0.0029 0.0068 0.0016 0.0023 0.0049 0.0034 0.002
Endosome 0.0373 0.004 0.0127 0.0115 0.0151 0.0047 0.0331 0.0042 0.0153 0.0381 0.0299 0.0129 0.0296 0.0063 0.0128 0.0242 0.0153 0.0093
Golgi 0.0178 0.0007 0.0029 0.0143 0.0044 0.0031 0.0087 0.0011 0.0065 0.0049 0.0136 0.0025 0.0083 0.0079 0.0039 0.0045 0.0018 0.002
Lipid Particles 0.0273 0.0006 0.0036 0.0044 0.0059 0.0078 0.007 0.0006 0.0024 0.0046 0.0229 0.0016 0.0129 0.0029 0.0067 0.019 0.0006 0.0016
Mitochondria 0.017 0.0002 0.0006 0.0077 0.0003 0.0008 0.0127 0.0002 0.0029 0.0013 0.0085 0.0006 0.0045 0.0013 0.0005 0.0006 0.0003 0.0003
None 0.0749 0.0394 0.0653 0.0581 0.0136 0.0428 0.0362 0.0211 0.0267 0.0338 0.0503 0.0301 0.0364 0.0163 0.0291 0.0068 0.0288 0.0101
Nuclear Periphery 0.0059 0.0009 0.0031 0.0013 0.0007 0.0011 0.0066 0.0005 0.0009 0.0016 0.0018 0.0012 0.006 0.0004 0.0007 0.0021 0.0007 0.0005
Nucleolus 0.0006 0.0001 0.0002 0.0001 0.0016 0.0156 0.0006 0.0001 0.0047 0.0003 0.0003 0.0006 0.0071 0.0004 0.0002 0.0091 0.0001 0.0002
Nucleus 0.0022 0.0013 0.0017 0.0013 0.0025 0.0061 0.0056 0.002 0.0028 0.005 0.0041 0.0041 0.0038 0.0021 0.0013 0.0045 0.001 0.0017
Peroxisomes 0.0197 0.0029 0.0079 0.0052 0.0033 0.0101 0.0189 0.0067 0.0323 0.0067 0.0209 0.0088 0.0223 0.0231 0.0326 0.0154 0.0125 0.0108
Punctate Nuclear 0.0055 0.0039 0.0186 0.0091 0.0346 0.0082 0.0064 0.0055 0.0028 0.0031 0.0042 0.0088 0.0118 0.0071 0.0104 0.0213 0.0399 0.0068
Vacuole 0.0026 0.0051 0.002 0.0009 0.0013 0.0031 0.0026 0.0019 0.0017 0.0023 0.0023 0.0015 0.0029 0.002 0.0026 0.0206 0.0009 0.0007
Vacuole Periphery 0.001 0.0005 0.0003 0.0003 0.0002 0.0002 0.0006 0.0002 0.0003 0.0005 0.0005 0.0002 0.0009 0.0001 0.0002 0.0009 0.0002 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 20.3162 22.6428 22.4026 32.9015 20.1832 40.0824 28.9753 33.1574 36.3801 31.8141
Translational Efficiency 1.2986 1.0336 1.0419 0.9513 1.2439 0.8072 0.911 0.9083 0.8514 0.8089

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1815 732 1552 898 1330 1765 630 1121 3145 2497 2182 2019

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 685.79 762.73 1156.29 1129.01 716.89 838.51 1033.42 1085.44 698.94 816.29 1120.81 1104.82
Standard Deviation 224.69 120.08 223.75 252.11 102.93 115.74 163.83 272.93 183.99 122.01 215.54 264.76
Intensity Change Log 2 — 0.153406 0.753665 0.719219 — 0.226076 0.527603 0.598456 — 0.191004 0.642554 0.658763

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000349 0.000649 0.037191 0.010344 0.000548 0.002206 0.000255 0.024671 0.000433 0.001750 0.026527 0.018299
Bud Neck 0.018054 0.016585 0.010787 0.011073 0.005905 0.030642 0.002217 0.008320 0.012916 0.026521 0.008313 0.009544
Bud Site 0.014031 0.033283 0.148459* 0.144379* 0.007706 0.062594 0.005907 0.154742* 0.011356 0.054001 0.107300* 0.150133*
Cell Periphery 0.000176 0.000118 0.000261 0.000212 0.000141 0.000186 0.000231 0.000275 0.000161 0.000166 0.000252 0.000247
Cytoplasm 0.565740* 0.538519* 0.245197* 0.315535* 0.631446* 0.443455* 0.529919* 0.379544* 0.593527* 0.471323* 0.327403* 0.351074*
Cytoplasmic Foci 0.223400* 0.235433* 0.258246* 0.151490 0.182712* 0.254306* 0.038244 0.177917 0.206193* 0.248773* 0.194726* 0.166163
Eisosomes 0.000198 0.000092 0.000055 0.000052 0.000087 0.000170 0.000122 0.000075 0.000151 0.000147 0.000074 0.000065
Endoplasmic Reticulum 0.000994 0.000411 0.001491 0.002714 0.001683 0.000593 0.002418 0.000507 0.001285 0.000539 0.001758 0.001488
Endosome 0.012986 0.023514 0.011250 0.023442 0.010709 0.031649 0.003813 0.009183 0.012023 0.029264 0.009103 0.015525
Golgi 0.003541 0.004290 0.003496 0.009582 0.002108 0.008959 0.000624 0.006188 0.002935 0.007590 0.002666 0.007698
Lipid Particles 0.004710 0.003174 0.008811 0.002268 0.004288 0.003090 0.002668 0.004430 0.004531 0.003114 0.007037 0.003468
Mitochondria 0.002447 0.003334 0.000458 0.002624 0.002364 0.005396 0.000467 0.000433 0.002412 0.004791 0.000461 0.001408
Mitotic Spindle 0.000410 0.008821 0.107789* 0.115715* 0.000862 0.012191 0.006760 0.059361 0.000601 0.011203 0.078619 0.084426
None 0.008201 0.004160 0.001253 0.000851 0.004916 0.001557 0.009819 0.002220 0.006812 0.002320 0.003726 0.001611
Nuclear Periphery 0.000149 0.000140 0.000314 0.000500 0.000496 0.000177 0.000904 0.000148 0.000296 0.000167 0.000485 0.000304
Nuclear Periphery Foci 0.000573 0.001206 0.005862 0.002175 0.001930 0.000510 0.002691 0.002363 0.001147 0.000714 0.004946 0.002279
Nucleolus 0.001312 0.000751 0.000382 0.000426 0.001630 0.000818 0.000568 0.000164 0.001446 0.000799 0.000435 0.000280
Nucleus 0.080269 0.040107 0.033649 0.015206 0.080364 0.029522 0.246550* 0.022215 0.080309 0.032625 0.095119 0.019097
Peroxisomes 0.005832 0.013220 0.091606 0.058799 0.004293 0.035014 0.002059 0.074412 0.005181 0.028625 0.065751 0.067468
Vacuole 0.055731 0.071048 0.033164 0.126010 0.054976 0.074926 0.143433 0.072515 0.055412 0.073789 0.065001 0.096308
Vacuole Periphery 0.000899 0.001144 0.000281 0.006604 0.000835 0.002040 0.000334 0.000319 0.000872 0.001777 0.000296 0.003114

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.63 -12.03 -3.71 -3.46 9.00 -2.70 0.91 -7.22 -6.17 -7.35 -3.12 -11.82 -8.12 -6.74 5.15
Bud Neck 0.79 3.36 4.99 2.99 2.65 -11.44 3.18 -1.93 11.09 -7.88 -7.65 2.82 4.13 11.06 1.82
Bud Site -4.59 -24.50* -13.90 -8.88 5.87 -15.71 -0.61 -18.28 -6.88 -17.83 -15.20 -23.46 -22.59 -9.92 -0.13
Cell Periphery 5.22 -5.74 1.14 -1.74 5.38 -2.46 -2.76 -5.74 -3.83 -3.26 -0.23 -6.72 -3.85 -3.41 1.81
Cytoplasm 2.31 30.91 17.95 12.11 -6.61 18.08 12.38 18.55 2.94 3.62 15.66 33.44 24.88 9.32 -6.15
Cytoplasmic Foci -1.11 0.43 14.86 12.16 12.48 -9.85 25.21 5.85 14.73 -15.53 -7.54 6.60 14.72 19.99 6.12
Eisosomes 5.16 6.89 7.82 8.00 4.16 -4.14 -0.16 2.10 5.07 0.98 0.33 5.99 7.61 6.52 1.55
Endoplasmic Reticulum 2.46 -3.41 -0.34 -1.41 1.38 3.06 -2.50 3.01 -0.47 5.80 3.55 -3.81 1.26 -1.36 4.65
Endosome -4.60 2.18 0.20 4.43 -1.58 -11.10 8.01 4.46 13.60 -2.33 -11.38 4.20 3.11 13.15 -0.59
Golgi -0.82 1.65 0.09 0.56 -0.65 -7.31 4.07 -0.62 4.30 -2.12 -6.40 2.55 -0.18 4.25 -1.32
Lipid Particles 3.56 -7.10 2.97 -0.73 8.87 3.03 3.57 -2.65 -4.35 -4.75 4.91 -5.87 -1.29 -4.30 3.78
Mitochondria -0.55 4.64 2.66 1.62 -1.39 -2.13 2.04 2.08 5.00 0.28 -2.45 4.08 3.55 4.97 -1.00
Mitotic Spindle -3.55 -19.79* -14.96 -13.25 -0.30 -6.15 -4.31 -11.26 -8.31 -5.86 -7.21 -19.73 -18.54 -15.19 0.36
None 2.94 7.14 7.00 2.62 -0.52 4.63 -0.74 3.86 -1.06 2.62 6.21 5.42 7.72 1.27 1.87
Nuclear Periphery 0.01 -5.63 -10.27 -10.28 -0.94 1.49 -3.66 0.51 -3.40 13.47 1.29 -4.90 -2.78 -7.51 3.94
Nuclear Periphery Foci -3.46 -9.12 -6.46 -4.48 5.20 5.80 -4.02 -4.93 -8.17 0.11 2.61 -9.56 -8.46 -9.60 4.73
Nucleolus 2.00 2.48 2.21 0.62 -0.54 2.07 2.33 3.43 3.14 1.96 2.67 3.51 4.13 2.65 1.41
Nucleus 11.51 9.81 17.42 3.88 5.63 16.37 -14.65 11.97 -3.24 19.67 22.15 -3.67 20.23 -0.29 16.01
Peroxisomes -4.11 -17.19 -7.96 -5.00 7.93 -12.68 2.56 -10.01 -1.25 -10.79 -13.17 -16.38 -12.68 -2.47 3.46
Vacuole -4.47 -4.09 -18.75 -15.19 -16.05 -5.07 -16.81 -15.43 -12.50 5.19 -6.99 -14.05 -24.13 -19.65 -10.86
Vacuole Periphery -1.21 8.75 -1.85 -1.50 -2.75 -2.73 3.12 3.80 4.54 0.61 -2.95 7.07 -0.68 1.53 -2.62
ORF
Human Ortholog
Description Putative metalloendopeptidase; forms cytoplasmic foci upon DNA replication stress
Localization
Cell Percentages cytoplasm (85%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

YIL108W

YIL108W


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Yil108w-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available