Standard name
Human Ortholog
Description AdoMet-dependent methyltransferase; involved in a novel 3-methylhistidine modification of ribosomal protein Rpl3p; seven beta-strand MTase family member; null mutant exhibits a weak vacuolar protein sorting defect and caspofungin resistance
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.9 0.83 0.95 0.95 0.95 0.95 0.87 0.92 0.87 0.85 0.69 0.8 0.93 0.94 0.97 0.07 0.07 0.06 0.71 0.72 0.71 0.77 0.71 0.74
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.05 0 0 0 0 0.07 0.18 0.09 0.22 0.17 0.44 0.21 0 0 0 0 0 0 0 0.05 0.08 0 0 0
Nucleus 0.05 0.09 0 0 0 0 0 0 0 0 0 0 0 0 0 0.73 0.66 0.71 0.07 0.12 0 0.05 0.06 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0.12 0 0 0 0 0 0 0 0 0 0 0 0 0 0.21 0.29 0.25 0 0.07 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 13 0 3 3 7 12
Bud 0 0 0 0 0 0 1 0 2 1 4 2 0 0 0 0 0 0 13 0 4 13 31 26
Bud Neck 1 0 10 5 8 5 5 16 4 9 2 13 1 2 3 0 0 0 3 1 0 2 5 7
Bud Site 0 0 0 0 0 0 0 0 1 1 2 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 0 3 0 0 2 0 1 1 1 2 2 2 0 0 0 0 0 4 0 3 2 6 6
Cytoplasm 624 114 338 330 445 419 406 409 410 382 239 307 417 518 567 10 16 13 483 104 230 373 484 479
Endoplasmic Reticulum 1 1 1 0 1 0 0 1 0 0 0 1 4 0 0 0 7 11 5 0 2 3 1 3
Endosome 1 0 1 0 0 0 0 0 0 1 0 1 1 2 2 1 7 4 10 2 3 3 5 5
Golgi 0 0 1 1 0 0 0 0 0 0 0 0 1 1 1 0 1 0 0 0 1 0 1 1
Mitochondria 33 1 0 2 4 32 82 41 105 78 154 82 1 0 0 3 2 7 26 7 26 13 26 17
Nucleus 32 13 11 15 17 10 13 10 7 13 11 8 18 21 9 99 150 159 51 16 10 24 39 19
Nuclear Periphery 1 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 2 2 3
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 5 2 0 0 0 1 0 0
Peroxisomes 0 0 0 0 1 1 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 1 1
SpindlePole 0 0 2 2 4 0 7 4 6 8 4 13 0 0 3 0 2 0 19 0 13 13 29 21
Vac/Vac Membrane 25 16 5 2 4 2 6 0 3 2 3 4 7 18 12 29 65 56 27 9 8 15 26 25
Unique Cell Count 696 137 357 348 468 440 466 443 471 450 347 383 447 552 584 135 228 225 683 145 324 483 684 647
Labelled Cell Count 719 145 373 357 484 471 521 483 539 496 421 433 452 562 598 144 256 252 683 145 324 483 684 647


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.4 4.3 4.6 4.3 4.3 3.5 3.4 3.9 3.1 3.2 2.9 3.1 4.7 5.1 5.0 8.1 9.0 8.5 4.6 4.7 5.2
Std Deviation (1e-4) 0.8 0.8 1.5 1.9 1.7 1.5 1.7 1.6 1.4 0.8 1.2 0.9 1.3 1.6 1.7 1.4 1.9 1.6 1.6 1.5 2.0
Intensity Change (Log2) – – – -0.11 -0.1 -0.41 -0.45 -0.26 -0.58 -0.53 -0.67 -0.56 0.01 0.14 0.1 0.81 0.96 0.88 -0.02 0.03 0.18


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 7.8 9.9 9.0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.5304 0.836 0.8315 0.5739 0.3509 0.5768 -0.5302 -0.124 -0.3974 -0.6541 -0.5937 -0.5384 0.5012 -0.485 -0.2451 -0.1783 0.0388 -0.3376
Actin 0.0077 0.0002 0.0097 0.0027 0.0261 0.001 0.0126 0.001 0.0015 0.0001 0.0001 0.0003 0.03 0.0067 0.0046 0.0075 0.0417 0.0037
Bud 0.0005 0.0017 0.0004 0.0005 0.0011 0.0018 0.0007 0.0007 0.0004 0.0006 0.0001 0.0002 0.0058 0.0016 0.009 0.0013 0.0103 0.0025
Bud Neck 0.0025 0.0004 0.0036 0.0017 0.0039 0.0031 0.0041 0.0006 0.0005 0.0004 0.0003 0.0111 0.0044 0.0015 0.0009 0.0057 0.0015 0.0026
Bud Periphery 0.0013 0.0008 0.0008 0.0008 0.0026 0.0038 0.0012 0.0005 0.0004 0.0006 0.0001 0.0001 0.004 0.0012 0.006 0.0044 0.0446 0.001
Bud Site 0.0024 0.0015 0.0075 0.0018 0.002 0.0004 0.0031 0.006 0.0044 0.0007 0.0001 0.0011 0.0071 0.0234 0.0101 0.006 0.0019 0.0008
Cell Periphery 0.0002 0.0001 0.0002 0.0003 0.0002 0.0001 0.0002 0.0002 0.0001 0.0001 0 0 0.0004 0.0002 0.0004 0.0002 0.0004 0.0001
Cytoplasm 0.0731 0.061 0.0441 0.0268 0.0668 0.0964 0.0557 0.0419 0.0576 0.0389 0.0645 0.0725 0.0834 0.1289 0.0857 0.057 0.0778 0.1373
Cytoplasmic Foci 0.0105 0.0047 0.0081 0.0074 0.0053 0.0113 0.0164 0.0086 0.0119 0.0015 0.0018 0.007 0.047 0.0141 0.0089 0.0292 0.0084 0.0166
Eisosomes 0.0002 0.0001 0.0003 0.0001 0.0003 0.0001 0.0005 0.0001 0.0001 0 0 0.0001 0.0004 0.0002 0.0002 0.0001 0.0001 0.0001
Endoplasmic Reticulum 0.0042 0.0011 0.0009 0.0018 0.0015 0.0011 0.0017 0.001 0.0011 0.0004 0.0003 0.0008 0.011 0.0017 0.0028 0.0016 0.0019 0.0015
Endosome 0.0087 0.0015 0.0056 0.0403 0.0065 0.0042 0.008 0.0006 0.0037 0.0003 0.0002 0.0008 0.0415 0.0089 0.009 0.0174 0.0216 0.0074
Golgi 0.0014 0.0005 0.0034 0.0159 0.0036 0.0015 0.0027 0.0001 0.0007 0 0 0 0.0079 0.0003 0.0032 0.0138 0.0072 0.003
Lipid Particles 0.0064 0.0021 0.0034 0.0015 0.0225 0.0034 0.0112 0.0002 0.0016 0.0001 0 0.0002 0.0426 0.0009 0.0097 0.0481 0.0136 0.0058
Mitochondria 0.0051 0.0007 0.0104 0.0104 0.0013 0.0016 0.0058 0.0003 0.0066 0.0005 0.0002 0.0003 0.0081 0.0011 0.0081 0.0267 0.0086 0.0025
None 0.586 0.5545 0.5929 0.6974 0.5407 0.5271 0.6923 0.674 0.6115 0.5844 0.6488 0.659 0.3711 0.5737 0.5384 0.272 0.5066 0.4601
Nuclear Periphery 0.0167 0.0061 0.0051 0.0081 0.0098 0.0062 0.007 0.0042 0.012 0.0027 0.0015 0.0028 0.0494 0.006 0.0107 0.015 0.0151 0.0109
Nucleolus 0.0049 0.003 0.0051 0.0025 0.0069 0.0063 0.0032 0.0026 0.0037 0.0048 0.0016 0.004 0.0071 0.0044 0.0016 0.0043 0.003 0.003
Nucleus 0.2192 0.3318 0.274 0.1659 0.2518 0.2778 0.1315 0.2419 0.2614 0.3497 0.2691 0.2159 0.2019 0.1862 0.2423 0.318 0.2103 0.2878
Peroxisomes 0.0101 0.0056 0.0092 0.0011 0.0015 0.0024 0.0197 0.0004 0.001 0 0 0.0001 0.0232 0.0025 0.029 0.036 0.0088 0.0067
Punctate Nuclear 0.0345 0.0213 0.0139 0.0084 0.0439 0.0484 0.02 0.0144 0.0176 0.0127 0.0111 0.023 0.0366 0.0329 0.0155 0.1318 0.0055 0.0449
Vacuole 0.0034 0.0013 0.001 0.0032 0.0011 0.0017 0.0019 0.0008 0.0017 0.0013 0.0002 0.0006 0.0103 0.0031 0.0022 0.0025 0.0078 0.0012
Vacuole Periphery 0.001 0.0002 0.0004 0.0015 0.0006 0.0004 0.0007 0.0001 0.0005 0.0001 0 0.0001 0.0065 0.0005 0.0017 0.0016 0.0032 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 37.4613 36.5325 38.7266 32.694 25.2313 30.1469 31.0015 24.6338 19.6753 33.0558
Translational Efficiency 0.9942 0.8906 0.8431 0.7206 1.0508 0.8337 0.944 0.9286 1.1158 0.7913

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
22 1765 3004 1523 2241 2262 188 565 2263 4027 3192 2088

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 951.80 705.99 817.60 784.22 634.64 670.78 885.49 838.50 637.72 686.21 821.60 798.91
Standard Deviation 297.14 93.40 125.30 152.16 74.26 86.01 121.18 142.64 85.37 91.02 126.08 151.57
Intensity Change Log 2 — -0.431011 -0.219263 -0.279400 — 0.079901 0.480538 0.401872 — -0.204505 0.102362 0.032621

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.003808 0.000309 0.000215 0.000565 0.000134 0.000168 0.000475 0.001128 0.000170 0.000230 0.000230 0.000718
Bud Neck 0.060224 0.029623 0.012793 0.013218 0.010891 0.023259 0.001672 0.010426 0.011371 0.026048 0.012138 0.012463
Bud Site 0.027565 0.009562 0.005091 0.029324 0.004274 0.009370 0.000860 0.022840 0.004500 0.009454 0.004841 0.027570
Cell Periphery 0.000652 0.000259 0.000129 0.000349 0.000196 0.000169 0.000177 0.000223 0.000201 0.000208 0.000132 0.000315
Cytoplasm 0.123175* 0.352273* 0.068942 0.288468* 0.414275* 0.433065* 0.069492 0.321140* 0.411445* 0.397655* 0.068974 0.297309*
Cytoplasmic Foci 0.032916 0.210005* 0.000386 0.012482 0.109016 0.142595 0.009397 0.020232 0.108276 0.172140 0.000917 0.014579
Eisosomes 0.000188 0.000118 0.000013 0.000041 0.000086 0.000087 0.000091 0.000063 0.000087 0.000101 0.000018 0.000047
Endoplasmic Reticulum 0.037698 0.001458 0.002743 0.004192 0.000730 0.000568 0.006484 0.003312 0.001089 0.000958 0.002963 0.003954
Endosome 0.012391 0.010619 0.000417 0.005884 0.003872 0.007689 0.001585 0.005152 0.003954 0.008973 0.000486 0.005686
Golgi 0.001897 0.003396 0.000033 0.002229 0.000845 0.002255 0.000073 0.002670 0.000855 0.002755 0.000035 0.002348
Lipid Particles 0.008048 0.009744 0.000175 0.001293 0.007601 0.005581 0.002983 0.001359 0.007606 0.007406 0.000340 0.001311
Mitochondria 0.008725 0.011228 0.001322 0.003059 0.004152 0.006858 0.000459 0.007017 0.004197 0.008774 0.001271 0.004130
Mitotic Spindle 0.001873 0.004183 0.002894 0.027809 0.002255 0.008872 0.000149 0.030761 0.002251 0.006817 0.002733 0.028608
None 0.011693 0.004475 0.004641 0.008719 0.005033 0.005708 0.008859 0.008615 0.005098 0.005167 0.004889 0.008691
Nuclear Periphery 0.005516 0.000968 0.002038 0.002259 0.000931 0.000761 0.001538 0.001774 0.000975 0.000852 0.002008 0.002128
Nuclear Periphery Foci 0.022119 0.000431 0.000113 0.001533 0.000257 0.000234 0.000644 0.002621 0.000470 0.000320 0.000144 0.001827
Nucleolus 0.061413 0.002764 0.000797 0.001760 0.002117 0.002215 0.002649 0.001665 0.002694 0.002456 0.000907 0.001734
Nucleus 0.403727* 0.290912* 0.890672* 0.537117* 0.411559* 0.308740* 0.865140* 0.477278* 0.411483* 0.300926* 0.889168* 0.520925*
Peroxisomes 0.004958 0.016726 0.000097 0.000516 0.003724 0.008578 0.000453 0.002450 0.003736 0.012149 0.000118 0.001039
Vacuole 0.138659 0.039732 0.006279 0.054690 0.017617 0.032110 0.026662 0.076672 0.018793 0.035450 0.007479 0.060638
Vacuole Periphery 0.032756 0.001215 0.000211 0.004493 0.000435 0.001119 0.000157 0.002603 0.000749 0.001161 0.000208 0.003981

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 2.73 2.77 2.55 -5.08 -6.59 -0.59 -4.91 -3.73 -3.71 -0.92 -0.97 -1.69 -5.60 -6.96 -5.99
Bud Neck 0.97 1.49 1.50 8.11 0.20 -7.48 8.78 -0.02 5.37 -4.50 -10.31 -1.35 -1.04 9.48 0.22
Bud Site 1.52 1.86 0.57 -4.46 -6.28 -3.61 2.74 -3.82 -1.96 -4.79 -4.51 -0.65 -7.38 -4.90 -7.07
Cell Periphery 1.67 2.33 1.68 -0.07 -5.95 1.21 -0.02 1.34 0.13 1.37 -0.27 3.53 -1.42 -1.10 -6.00
Cytoplasm -5.08 1.17 -2.66 11.90 -24.39 -1.91 30.83 14.57 15.66 -11.96 2.31 56.94 21.42 21.54 -28.41
Cytoplasmic Foci -10.73 1.99 1.35 36.57 -11.79 -6.53 27.52 27.04 30.79 -1.95 -13.64 32.75 29.01 47.70 -13.99
Eisosomes 1.30 3.35 2.65 13.96 -19.31 -0.18 -2.09 1.55 1.74 3.16 -2.45 13.84 5.86 11.65 -19.59
Endoplasmic Reticulum 2.94 2.79 2.62 -12.05 -6.31 1.82 -9.28 -10.58 -11.01 3.71 0.65 -12.14 -14.79 -17.35 -6.02
Endosome 0.48 3.67 2.49 7.70 -6.60 -6.22 4.60 0.84 6.00 -2.52 -9.75 11.09 0.13 8.61 -7.94
Golgi -1.81 2.94 0.91 3.68 -3.02 -2.86 4.87 -0.11 1.65 -1.32 -5.35 5.27 -0.79 3.37 -3.30
Lipid Particles -0.82 3.27 2.77 13.45 -15.17 2.78 5.67 10.31 8.67 3.65 0.27 12.68 10.82 15.91 -14.21
Mitochondria -0.65 1.95 1.70 8.30 -3.20 -2.78 5.32 0.69 2.15 -1.39 -5.09 4.19 2.04 8.04 -2.21
Mitotic Spindle -1.81 -1.59 -7.24 -6.62 -6.82 -4.13 2.82 -4.84 -3.06 -5.37 -4.16 -1.50 -8.69 -6.48 -8.12
None 0.90 0.92 0.62 -2.71 -3.22 -0.82 -0.93 -1.74 -1.01 0.35 -0.04 1.10 -2.11 -2.32 -3.48
Nuclear Periphery 1.66 1.13 0.89 -13.23 -3.54 2.53 -6.27 -12.81 -13.94 -4.41 1.43 -9.88 -18.20 -19.74 -4.17
Nuclear Periphery Foci 2.09 2.12 1.98 -3.61 -4.63 0.25 -4.79 -3.52 -3.55 -2.03 1.17 2.46 -4.25 -5.13 -5.70
Nucleolus 3.04 3.15 3.11 4.19 -3.15 -0.45 -0.38 2.16 3.09 2.09 0.37 4.56 3.09 4.84 -2.86
Nucleus 1.94 -8.14 -2.53 -24.41 35.94 11.96 -27.23 -6.54 -13.41 16.52 14.50 -68.92 -14.00 -28.46 42.78
Peroxisomes -6.82 3.75 3.54 13.93 -3.91 -5.09 5.74 3.31 7.74 -0.72 -9.53 6.42 5.48 16.77 -2.17
Vacuole 2.36 3.11 0.89 -12.52 -19.91 -9.16 -4.13 -14.84 -13.11 -9.05 -11.49 5.46 -22.49 -18.43 -24.41
Vacuole Periphery 2.26 2.33 2.16 -1.68 -2.94 -2.95 5.27 -1.07 0.25 -1.55 -1.98 3.52 -2.24 -1.60 -3.21
Standard name
Human Ortholog
Description AdoMet-dependent methyltransferase; involved in a novel 3-methylhistidine modification of ribosomal protein Rpl3p; seven beta-strand MTase family member; null mutant exhibits a weak vacuolar protein sorting defect and caspofungin resistance
Localization
Cell Percentages nucleus (27%), cytoplasm (12%), mixed (41%)
Cell Cycle Regulation Yes
cytoplasm - MA (0.00023)
nucleus - T/G1 (0.00079)
Subcompartmental Group N/A

Hpm1

Hpm1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Hpm1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available