Standard name
Human Ortholog
Description Subunit of the Set3 complex; complex is a meiotic-specific repressor of sporulation specific genes that contains deacetylase activity; potential Cdc28p substrate

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0.05 0 0 0.05 0 0 0.06 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.11 0.13 0.09 0.07 0.09 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.14 0.28 0.38 0.17 0.41 0.36 0.42 0.52 0.49 0.43 0.54 0.05 0.05 0.2 0.08 0 0.15 0 0 0 0 0 0
Nucleus 0.87 0.84 0.83 0.88 0.84 0.74 0.75 0.71 0.65 0.63 0.63 0.63 0.85 0.83 0.77 0.88 0.84 0.83 0.73 0.74 0.71 0.79 0.72 0.61
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.18 0.22 0.16 0.13 0.16 0.24 0.25 0.25 0.29 0.33 0.35 0.38 0.14 0.11 0.17 0.05 0.05 0.05 0.21 0.13 0.15 0.14 0.18 0.28
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.05 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0
Bud 0 0 1 1 0 4 4 4 7 4 3 3 0 0 1 0 0 0 1 3 3 2 2 2
Bud Neck 0 0 1 0 1 0 0 3 0 1 0 0 0 1 0 0 0 0 1 0 0 0 0 0
Bud Site 0 0 3 7 1 17 19 16 19 19 11 20 0 1 3 0 0 0
Cell Periphery 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 3 9 4 6 9 10 15 18 15 14 8 9 33 52 65 9 7 10 0 4 0 0 0 1
Endoplasmic Reticulum 1 2 2 0 0 0 0 0 0 0 0 0 0 3 1 2 2 1 0 0 2 0 0 1
Endosome 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 3 2 1 6 1 1 1 5
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 1 0 0 2 4 0 1 1
Mitochondria 0 40 62 100 56 156 192 176 188 213 112 185 21 25 96 8 3 16 0 3 4 0 1 2
Nucleus 120 243 185 229 279 279 399 300 237 275 165 216 330 386 374 90 80 90 106 223 167 174 234 206
Nuclear Periphery 0 6 6 4 3 2 9 4 7 14 5 3 1 3 2 1 0 0 0 0 0 0 0 0
Nucleolus 25 63 35 34 52 92 130 107 105 147 92 132 54 53 84 5 5 5 30 39 34 30 57 93
Peroxisomes 0 0 0 0 0 0 1 0 0 0 0 0 1 2 1 0 0 0 0 0 0 0 0 0
SpindlePole 1 2 3 2 3 1 7 4 2 2 3 4 2 7 3 0 0 2 0 4 3 4 3 5
Vac/Vac Membrane 2 5 2 0 3 3 2 1 2 3 0 1 2 6 2 1 4 0 3 10 12 5 16 11
Unique Cell Count 138 288 224 260 333 376 529 423 365 439 260 343 387 466 484 102 95 109 147 303 237 222 324 337
Labelled Cell Count 153 371 304 384 407 564 778 633 582 692 399 573 445 539 632 117 105 126 147 303 237 222 324 337


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.9 5.6 4.8 4.6 4.8 4.4 4.3 4.2 4.1 4.1 4.0 3.7 5.8 5.6 5.0 8.2 8.4 8.1 6.0 5.9 6.6
Std Deviation (1e-4) 1.1 1.1 1.1 1.2 1.5 1.3 1.0 1.2 1.6 1.5 1.1 0.9 1.2 1.3 1.1 2.1 2.0 1.4 1.2 1.0 1.3
Intensity Change (Log2) -0.07 0.01 -0.13 -0.18 -0.18 -0.24 -0.25 -0.27 -0.4 0.28 0.21 0.05 0.76 0.8 0.75 0.33 0.28 0.46


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.7 0.7 0.8 1.6 1.5 1.1 0.9 0.7 3.4 4.2 4.9 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 -7.7 -8.3 -2.3 -4.0 -5.0 -2.6
Nucleus 1.7 0.4 -2.4 -2.2 -3.3 -4.6 -5.3 -4.7 -5.0 0.9 0.1 -1.6 1.3 0.4 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 -0.6 -1.6 0.6 -2.7 -2.6 -2.9
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.0137 2.3992 2.0529 1.9469 2.3982 2.3413 3.3942 3.0837 3.1722 2.8806 2.4392 2.8629 3.6907 3.9878 3.8639 3.5022 3.1034 3.8355
Actin 0.0421 0 0.0055 0 0.0268 0.0037 0.02 0 0.0217 0.0469 0.0005 0.0024 0.0001 0 0.0053 0 0.0001 0
Bud 0.0013 0.0001 0.0001 0 0.0006 0.0001 0.001 0.0001 0.0008 0 0.0001 0 0.0001 0 0 0.0001 0 0
Bud Neck 0.0057 0.0005 0.0004 0.0002 0.0014 0.0118 0.0039 0.0001 0.0012 0.0001 0.001 0.0006 0.0004 0.0001 0.0001 0.0001 0.0009 0.0011
Bud Periphery 0.0044 0.0001 0.0001 0.0001 0.0012 0.0002 0.0023 0.0001 0.0012 0 0.0001 0 0.0002 0 0 0.0001 0 0.0001
Bud Site 0.0054 0.0005 0.0004 0.0001 0.002 0.0015 0.0068 0.0006 0.0096 0.0001 0.0003 0.0001 0.0003 0.0001 0.0001 0.0001 0.0001 0.0001
Cell Periphery 0.0012 0 0 0 0.0002 0.0001 0.0003 0 0.0024 0 0 0 0.0001 0 0 0 0 0
Cytoplasm 0.0049 0.0001 0.0095 0.0001 0.0008 0.0002 0.0134 0 0.0082 0 0.0011 0.0004 0.0004 0.0005 0.0001 0.0001 0.0001 0.0001
Cytoplasmic Foci 0.0098 0 0.0028 0 0.0083 0.0033 0.0191 0 0.0086 0.0018 0.0038 0.0014 0 0 0.0078 0 0 0
Eisosomes 0.0004 0 0.0001 0 0.0002 0.0001 0.0002 0 0.0006 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0049 0 0.0003 0 0.0023 0.0011 0.0024 0 0.0023 0 0.0003 0.0008 0.0004 0 0.0001 0 0 0
Endosome 0.0181 0.0001 0.0033 0.0001 0.0569 0.008 0.0171 0.0001 0.0152 0 0.0027 0.0043 0.0002 0.0002 0.0016 0 0 0
Golgi 0.0085 0 0.0034 0 0.0329 0.0058 0.0036 0 0.0037 0.0005 0.0006 0.001 0 0 0.0015 0 0 0
Lipid Particles 0.0117 0 0.0032 0 0.0408 0.0084 0.0091 0 0.0032 0.0007 0.008 0.0018 0 0 0.004 0 0 0
Mitochondria 0.0207 0.0002 0.0149 0.0002 0.0228 0.0028 0.0062 0.0003 0.0018 0.0001 0.0003 0.0002 0.0006 0.0003 0.001 0.0003 0.0001 0.0001
None 0.0025 0 0.003 0.0001 0.0009 0.0001 0.0148 0 0.0016 0 0.0005 0.0002 0.0001 0.0001 0.0003 0 0.0001 0.0001
Nuclear Periphery 0.0406 0.0082 0.002 0.0007 0.0097 0.0012 0.0245 0.0003 0.0049 0.0001 0.006 0.0011 0.002 0.0042 0.0003 0.002 0.0019 0.0003
Nucleolus 0.084 0.0682 0.047 0.0773 0.1211 0.1384 0.0731 0.0604 0.0348 0.0697 0.1184 0.09 0.0721 0.0457 0.0397 0.0373 0.1427 0.1136
Nucleus 0.6928 0.92 0.8804 0.9201 0.6492 0.7963 0.7436 0.9356 0.863 0.8668 0.8054 0.8898 0.9195 0.9176 0.9251 0.9551 0.842 0.8662
Peroxisomes 0.0065 0 0.0014 0 0.0037 0.0028 0.0098 0 0.0056 0.0121 0.0007 0.0003 0 0 0.0046 0 0 0
Punctate Nuclear 0.0119 0.0016 0.0205 0.0006 0.0106 0.0132 0.0233 0.002 0.0053 0.0008 0.0498 0.0052 0.0028 0.0295 0.0081 0.0043 0.0118 0.0183
Vacuole 0.0154 0.0002 0.0014 0.0002 0.0043 0.0004 0.0038 0.0001 0.003 0 0.0002 0.0001 0.0005 0.0012 0 0.0002 0 0.0001
Vacuole Periphery 0.0076 0.0001 0.0003 0.0001 0.0034 0.0004 0.0014 0.0001 0.0012 0 0.0001 0.0001 0.0002 0.0003 0 0.0001 0 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 13.4024 18.5021 13.9545 14.2051 15.1772 11.5298 18.9375 17.2835 18.3138 17.5518
Translational Efficiency 1.1291 1.0216 1.0877 1.4995 1.152 1.2938 1.027 1.1003 1.0103 0.8403

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Subunit of the Set3 complex; complex is a meiotic-specific repressor of sporulation specific genes that contains deacetylase activity; potential Cdc28p substrate
Localization
Cell Percentages nucleus (94%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Hos4

Hos4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Hos4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available