Standard name | |
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Human Ortholog | |
Description | Subunit of the Set3 complex; complex is a meiotic-specific repressor of sporulation specific genes that contains deacetylase activity; potential Cdc28p substrate |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 1 | 1 | 0 | 4 | 4 | 4 | 7 | 4 | 3 | 3 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 3 | 3 | 2 | 2 | 2 |
Bud Neck | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 3 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 3 | 7 | 1 | 17 | 19 | 16 | 19 | 19 | 11 | 20 | 0 | 1 | 3 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 3 | 9 | 4 | 6 | 9 | 10 | 15 | 18 | 15 | 14 | 8 | 9 | 33 | 52 | 65 | 9 | 7 | 10 | 0 | 4 | 0 | 0 | 0 | 1 |
Endoplasmic Reticulum | 1 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 1 | 2 | 2 | 1 | 0 | 0 | 2 | 0 | 0 | 1 |
Endosome | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 2 | 1 | 6 | 1 | 1 | 1 | 5 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 2 | 4 | 0 | 1 | 1 |
Mitochondria | 0 | 40 | 62 | 100 | 56 | 156 | 192 | 176 | 188 | 213 | 112 | 185 | 21 | 25 | 96 | 8 | 3 | 16 | 0 | 3 | 4 | 0 | 1 | 2 |
Nucleus | 120 | 243 | 185 | 229 | 279 | 279 | 399 | 300 | 237 | 275 | 165 | 216 | 330 | 386 | 374 | 90 | 80 | 90 | 106 | 223 | 167 | 174 | 234 | 206 |
Nuclear Periphery | 0 | 6 | 6 | 4 | 3 | 2 | 9 | 4 | 7 | 14 | 5 | 3 | 1 | 3 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 25 | 63 | 35 | 34 | 52 | 92 | 130 | 107 | 105 | 147 | 92 | 132 | 54 | 53 | 84 | 5 | 5 | 5 | 30 | 39 | 34 | 30 | 57 | 93 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 1 | 2 | 3 | 2 | 3 | 1 | 7 | 4 | 2 | 2 | 3 | 4 | 2 | 7 | 3 | 0 | 0 | 2 | 0 | 4 | 3 | 4 | 3 | 5 |
Vac/Vac Membrane | 2 | 5 | 2 | 0 | 3 | 3 | 2 | 1 | 2 | 3 | 0 | 1 | 2 | 6 | 2 | 1 | 4 | 0 | 3 | 10 | 12 | 5 | 16 | 11 |
Unique Cell Count | 138 | 288 | 224 | 260 | 333 | 376 | 529 | 423 | 365 | 439 | 260 | 343 | 387 | 466 | 484 | 102 | 95 | 109 | 147 | 303 | 237 | 222 | 324 | 337 |
Labelled Cell Count | 153 | 371 | 304 | 384 | 407 | 564 | 778 | 633 | 582 | 692 | 399 | 573 | 445 | 539 | 632 | 117 | 105 | 126 | 147 | 303 | 237 | 222 | 324 | 337 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.9 | 5.6 | 4.8 | 4.6 | 4.8 | 4.4 | 4.3 | 4.2 | 4.1 | 4.1 | 4.0 | 3.7 | 5.8 | 5.6 | 5.0 | 8.2 | 8.4 | 8.1 | 6.0 | 5.9 | 6.6 |
Std Deviation (1e-4) | 1.1 | 1.1 | 1.1 | 1.2 | 1.5 | 1.3 | 1.0 | 1.2 | 1.6 | 1.5 | 1.1 | 0.9 | 1.2 | 1.3 | 1.1 | 2.1 | 2.0 | 1.4 | 1.2 | 1.0 | 1.3 |
Intensity Change (Log2) | – | – | – | -0.07 | 0.01 | -0.13 | -0.18 | -0.18 | -0.24 | -0.25 | -0.27 | -0.4 | 0.28 | 0.21 | 0.05 | 0.76 | 0.8 | 0.75 | 0.33 | 0.28 | 0.46 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 0.7 | 0.7 | 0.8 | 1.6 | 1.5 | 1.1 | 0.9 | 0.7 | 3.4 | 4.2 | 4.9 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -7.7 | -8.3 | -2.3 | -4.0 | -5.0 | -2.6 |
Nucleus | 1.7 | 0.4 | -2.4 | -2.2 | -3.3 | -4.6 | -5.3 | -4.7 | -5.0 | 0.9 | 0.1 | -1.6 | 1.3 | 0.4 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -0.6 | -1.6 | 0.6 | -2.7 | -2.6 | -2.9 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.0137 | 2.3992 | 2.0529 | 1.9469 | 2.3982 | 2.3413 | 3.3942 | 3.0837 | 3.1722 | 2.8806 | 2.4392 | 2.8629 | 3.6907 | 3.9878 | 3.8639 | 3.5022 | 3.1034 | 3.8355 |
Actin | 0.0421 | 0 | 0.0055 | 0 | 0.0268 | 0.0037 | 0.02 | 0 | 0.0217 | 0.0469 | 0.0005 | 0.0024 | 0.0001 | 0 | 0.0053 | 0 | 0.0001 | 0 |
Bud | 0.0013 | 0.0001 | 0.0001 | 0 | 0.0006 | 0.0001 | 0.001 | 0.0001 | 0.0008 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0 |
Bud Neck | 0.0057 | 0.0005 | 0.0004 | 0.0002 | 0.0014 | 0.0118 | 0.0039 | 0.0001 | 0.0012 | 0.0001 | 0.001 | 0.0006 | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0.0009 | 0.0011 |
Bud Periphery | 0.0044 | 0.0001 | 0.0001 | 0.0001 | 0.0012 | 0.0002 | 0.0023 | 0.0001 | 0.0012 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0 | 0.0001 | 0 | 0.0001 |
Bud Site | 0.0054 | 0.0005 | 0.0004 | 0.0001 | 0.002 | 0.0015 | 0.0068 | 0.0006 | 0.0096 | 0.0001 | 0.0003 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 |
Cell Periphery | 0.0012 | 0 | 0 | 0 | 0.0002 | 0.0001 | 0.0003 | 0 | 0.0024 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.0049 | 0.0001 | 0.0095 | 0.0001 | 0.0008 | 0.0002 | 0.0134 | 0 | 0.0082 | 0 | 0.0011 | 0.0004 | 0.0004 | 0.0005 | 0.0001 | 0.0001 | 0.0001 | 0.0001 |
Cytoplasmic Foci | 0.0098 | 0 | 0.0028 | 0 | 0.0083 | 0.0033 | 0.0191 | 0 | 0.0086 | 0.0018 | 0.0038 | 0.0014 | 0 | 0 | 0.0078 | 0 | 0 | 0 |
Eisosomes | 0.0004 | 0 | 0.0001 | 0 | 0.0002 | 0.0001 | 0.0002 | 0 | 0.0006 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0049 | 0 | 0.0003 | 0 | 0.0023 | 0.0011 | 0.0024 | 0 | 0.0023 | 0 | 0.0003 | 0.0008 | 0.0004 | 0 | 0.0001 | 0 | 0 | 0 |
Endosome | 0.0181 | 0.0001 | 0.0033 | 0.0001 | 0.0569 | 0.008 | 0.0171 | 0.0001 | 0.0152 | 0 | 0.0027 | 0.0043 | 0.0002 | 0.0002 | 0.0016 | 0 | 0 | 0 |
Golgi | 0.0085 | 0 | 0.0034 | 0 | 0.0329 | 0.0058 | 0.0036 | 0 | 0.0037 | 0.0005 | 0.0006 | 0.001 | 0 | 0 | 0.0015 | 0 | 0 | 0 |
Lipid Particles | 0.0117 | 0 | 0.0032 | 0 | 0.0408 | 0.0084 | 0.0091 | 0 | 0.0032 | 0.0007 | 0.008 | 0.0018 | 0 | 0 | 0.004 | 0 | 0 | 0 |
Mitochondria | 0.0207 | 0.0002 | 0.0149 | 0.0002 | 0.0228 | 0.0028 | 0.0062 | 0.0003 | 0.0018 | 0.0001 | 0.0003 | 0.0002 | 0.0006 | 0.0003 | 0.001 | 0.0003 | 0.0001 | 0.0001 |
None | 0.0025 | 0 | 0.003 | 0.0001 | 0.0009 | 0.0001 | 0.0148 | 0 | 0.0016 | 0 | 0.0005 | 0.0002 | 0.0001 | 0.0001 | 0.0003 | 0 | 0.0001 | 0.0001 |
Nuclear Periphery | 0.0406 | 0.0082 | 0.002 | 0.0007 | 0.0097 | 0.0012 | 0.0245 | 0.0003 | 0.0049 | 0.0001 | 0.006 | 0.0011 | 0.002 | 0.0042 | 0.0003 | 0.002 | 0.0019 | 0.0003 |
Nucleolus | 0.084 | 0.0682 | 0.047 | 0.0773 | 0.1211 | 0.1384 | 0.0731 | 0.0604 | 0.0348 | 0.0697 | 0.1184 | 0.09 | 0.0721 | 0.0457 | 0.0397 | 0.0373 | 0.1427 | 0.1136 |
Nucleus | 0.6928 | 0.92 | 0.8804 | 0.9201 | 0.6492 | 0.7963 | 0.7436 | 0.9356 | 0.863 | 0.8668 | 0.8054 | 0.8898 | 0.9195 | 0.9176 | 0.9251 | 0.9551 | 0.842 | 0.8662 |
Peroxisomes | 0.0065 | 0 | 0.0014 | 0 | 0.0037 | 0.0028 | 0.0098 | 0 | 0.0056 | 0.0121 | 0.0007 | 0.0003 | 0 | 0 | 0.0046 | 0 | 0 | 0 |
Punctate Nuclear | 0.0119 | 0.0016 | 0.0205 | 0.0006 | 0.0106 | 0.0132 | 0.0233 | 0.002 | 0.0053 | 0.0008 | 0.0498 | 0.0052 | 0.0028 | 0.0295 | 0.0081 | 0.0043 | 0.0118 | 0.0183 |
Vacuole | 0.0154 | 0.0002 | 0.0014 | 0.0002 | 0.0043 | 0.0004 | 0.0038 | 0.0001 | 0.003 | 0 | 0.0002 | 0.0001 | 0.0005 | 0.0012 | 0 | 0.0002 | 0 | 0.0001 |
Vacuole Periphery | 0.0076 | 0.0001 | 0.0003 | 0.0001 | 0.0034 | 0.0004 | 0.0014 | 0.0001 | 0.0012 | 0 | 0.0001 | 0.0001 | 0.0002 | 0.0003 | 0 | 0.0001 | 0 | 0 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 13.4024 | 18.5021 | 13.9545 | 14.2051 | 15.1772 | 11.5298 | 18.9375 | 17.2835 | 18.3138 | 17.5518 |
Translational Efficiency | 1.1291 | 1.0216 | 1.0877 | 1.4995 | 1.152 | 1.2938 | 1.027 | 1.1003 | 1.0103 | 0.8403 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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