Standard name | |
---|---|
Human Ortholog | |
Description | Stress-inducible dual-specificity MAP kinase phosphatase; negatively regulates Slt2p MAP kinase by direct dephosphorylation, diffuse localization under normal conditions shifts to punctate localization after heat shock; SDP1 has a paralog, MSG5, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 5 | 11 | 11 | 10 | 10 | 1 | 0 | – | 0 | 1 | 1 | 2 | 16 | 10 | 14 | 16 | 6 | 5 | 6 | 3 | 2 | 8 | 27 | 30 |
Bud | 2 | 4 | 1 | 14 | 5 | 5 | 7 | – | 14 | 16 | 7 | 3 | 5 | 5 | 2 | 4 | 2 | 3 | 0 | 0 | 0 | 0 | 3 | 3 |
Bud Neck | 0 | 0 | 1 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 1 | 1 | 0 | 4 | 3 |
Bud Site | 0 | 0 | 0 | 1 | 0 | 4 | 2 | – | 6 | 3 | 2 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 6 | 7 | 8 | 13 | 7 | 5 | 7 | – | 3 | 6 | 2 | 2 | 17 | 26 | 16 | 34 | 11 | 12 | 0 | 0 | 0 | 0 | 2 | 2 |
Cytoplasm | 5 | 12 | 8 | 23 | 21 | 15 | 16 | – | 12 | 8 | 6 | 3 | 14 | 30 | 38 | 50 | 26 | 34 | 1 | 1 | 0 | 0 | 1 | 1 |
Endoplasmic Reticulum | 11 | 8 | 5 | 3 | 2 | 8 | 0 | – | 11 | 6 | 6 | 0 | 15 | 19 | 27 | 14 | 9 | 10 | 1 | 4 | 1 | 1 | 5 | 8 |
Endosome | 36 | 2 | 27 | 43 | 81 | 5 | 95 | – | 6 | 35 | 3 | 10 | 36 | 46 | 50 | 33 | 21 | 31 | 33 | 17 | 14 | 36 | 43 | 45 |
Golgi | 56 | 7 | 19 | 36 | 35 | 3 | 7 | – | 7 | 10 | 4 | 1 | 96 | 84 | 145 | 34 | 20 | 12 | 36 | 9 | 5 | 29 | 77 | 70 |
Mitochondria | 172 | 97 | 47 | 181 | 105 | 287 | 283 | – | 349 | 377 | 258 | 328 | 176 | 173 | 147 | 30 | 18 | 16 | 198 | 87 | 146 | 206 | 295 | 295 |
Nucleus | 0 | 0 | 1 | 4 | 2 | 6 | 15 | – | 9 | 5 | 5 | 5 | 0 | 0 | 2 | 3 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 |
Nuclear Periphery | 9 | 1 | 1 | 0 | 1 | 3 | 13 | – | 18 | 21 | 8 | 21 | 4 | 2 | 0 | 1 | 0 | 0 | 8 | 0 | 2 | 5 | 7 | 8 |
Nucleolus | 4 | 0 | 1 | 1 | 5 | 6 | 5 | – | 15 | 14 | 14 | 9 | 6 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 |
Peroxisomes | 3 | 1 | 5 | 15 | 14 | 0 | 17 | – | 2 | 2 | 1 | 1 | 8 | 11 | 6 | 4 | 1 | 6 | 6 | 3 | 7 | 17 | 42 | 37 |
SpindlePole | 6 | 0 | 16 | 6 | 28 | 4 | 35 | – | 15 | 14 | 6 | 12 | 7 | 3 | 2 | 12 | 12 | 7 | 3 | 1 | 5 | 1 | 5 | 7 |
Vac/Vac Membrane | 51 | 14 | 29 | 22 | 78 | 44 | 129 | – | 45 | 38 | 16 | 21 | 29 | 51 | 63 | 49 | 28 | 26 | 8 | 11 | 1 | 8 | 15 | 13 |
Unique Cell Count | 259 | 123 | 128 | 272 | 303 | 331 | 479 | 402 | 449 | 288 | 360 | 292 | 332 | 411 | 227 | 125 | 131 | 312 | 148 | 192 | 323 | 539 | 540 | |
Labelled Cell Count | 366 | 164 | 180 | 372 | 394 | 396 | 631 | 512 | 556 | 339 | 421 | 429 | 461 | 514 | 284 | 156 | 163 | 312 | 148 | 192 | 323 | 539 | 540 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.0 | 6.1 | 6.9 | 6.9 | 7.2 | 5.0 | 6.4 | – | 5.5 | 5.8 | 5.3 | 5.5 | 7.6 | 7.3 | 7.7 | 7.1 | 7.6 | 7.3 | 6.9 | 7.1 | 7.5 |
Std Deviation (1e-4) | 1.0 | 0.9 | 1.0 | 1.4 | 1.4 | 1.1 | 1.5 | – | 1.1 | 1.2 | 1.0 | 1.0 | 1.3 | 1.7 | 1.3 | 1.2 | 1.2 | 1.2 | 1.2 | 1.4 | 2.3 |
Intensity Change (Log2) | – | – | – | 0.0 | 0.07 | -0.45 | -0.11 | – | -0.33 | -0.24 | -0.39 | -0.31 | 0.15 | 0.09 | 0.17 | 0.05 | 0.13 | 0.08 | 0.01 | 0.05 | 0.13 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -2.1 | -2.3 | 0 | 0 | – | 0 | 0 | 0 | 0 | -1.2 | -2.6 | -2.4 | -0.5 | -1.2 | -1.6 |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -0.6 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | -0.2 | 0.6 | -1.1 | 2.4 | 0.8 | 0.9 |
Cytoplasm | 0.8 | 0.3 | -0.8 | -1.5 | – | 0 | 0 | 0 | 0 | -0.6 | 1.0 | 1.1 | 3.9 | 3.4 | 4.3 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0.5 | 0.8 | 1.1 | 0.9 | 1.1 | 1.3 |
Endosome | -1.3 | 1.2 | -7.4 | -0.3 | – | -8.0 | -4.3 | -7.3 | -6.7 | -2.3 | -1.9 | -2.5 | -1.6 | -0.9 | 0.5 |
Golgi | -0.4 | -0.9 | -6.3 | -6.6 | – | -6.0 | -5.8 | -5.5 | -7.1 | 3.8 | 2.4 | 4.4 | 0 | 0.3 | -1.4 |
Mitochondria | 5.6 | -0.4 | 10.8 | 4.5 | – | 11.4 | 10.7 | 11.3 | 12.5 | 4.5 | 3.0 | -0.2 | -5.2 | -4.1 | -4.6 |
Nucleus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 2.4 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0.7 | 0.3 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.3 |
SpindlePole | -4.2 | -1.0 | -5.3 | -1.9 | – | -3.7 | -4.2 | -4.4 | -3.8 | -4.2 | -5.6 | 0 | -2.4 | -0.7 | -2.0 |
Vacuole | -4.1 | 0.7 | -2.5 | 1.0 | – | -3.3 | -4.4 | -5.2 | -5.4 | -3.5 | -1.8 | -1.9 | -0.2 | 0 | -0.6 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.5864 | 1.8734 | 1.5876 | 1.2787 | 0.9918 | 1.442 | 4.1523 | 4.4157 | 3.6438 | 3.8745 | 3.2498 | 3.6734 | 4.7445 | 5.0157 | 4.9198 | 5.4361 | 4.3038 | 4.8811 |
Actin | 0.0171 | 0.0161 | 0.0099 | 0.0091 | 0.0058 | 0.0051 | 0.0563 | 0.0729 | 0.0295 | 0.0046 | 0.0148 | 0.0259 | 0.0471 | 0.0205 | 0.0212 | 0.0179 | 0.016 | 0.0142 |
Bud | 0.0018 | 0.0013 | 0.0016 | 0.0016 | 0.0052 | 0.0019 | 0.0009 | 0.0056 | 0.0014 | 0.0009 | 0.0013 | 0.0006 | 0.0008 | 0.0016 | 0.0007 | 0.0008 | 0.0018 | 0.0013 |
Bud Neck | 0.0004 | 0.0004 | 0.0007 | 0.0004 | 0.0008 | 0.0012 | 0.0009 | 0.001 | 0.0007 | 0.0003 | 0.0007 | 0.001 | 0.0022 | 0.0006 | 0.0007 | 0.0004 | 0.0005 | 0.0009 |
Bud Periphery | 0.0023 | 0.0018 | 0.0025 | 0.0045 | 0.006 | 0.0017 | 0.0016 | 0.0065 | 0.0026 | 0.003 | 0.0028 | 0.0014 | 0.0016 | 0.002 | 0.0013 | 0.0019 | 0.0064 | 0.0065 |
Bud Site | 0.0026 | 0.0064 | 0.0046 | 0.0011 | 0.0062 | 0.0009 | 0.002 | 0.0294 | 0.0073 | 0.0008 | 0.0019 | 0.001 | 0.0044 | 0.0083 | 0.0023 | 0.0008 | 0.0025 | 0.0009 |
Cell Periphery | 0.0006 | 0.0004 | 0.0005 | 0.0003 | 0.0007 | 0.0004 | 0.0005 | 0.0019 | 0.0013 | 0.0002 | 0.0003 | 0.0007 | 0.0007 | 0.0006 | 0.0005 | 0.0003 | 0.0009 | 0.0006 |
Cytoplasm | 0.002 | 0.0086 | 0.0031 | 0.0022 | 0.0102 | 0.0035 | 0.0056 | 0.0096 | 0.0105 | 0.0003 | 0.0086 | 0.0014 | 0.0077 | 0.0212 | 0.0033 | 0.0009 | 0.0106 | 0.0037 |
Cytoplasmic Foci | 0.0269 | 0.0313 | 0.0249 | 0.0245 | 0.0366 | 0.0283 | 0.0267 | 0.0431 | 0.0263 | 0.0037 | 0.0404 | 0.0145 | 0.0301 | 0.0384 | 0.0251 | 0.0074 | 0.0437 | 0.0244 |
Eisosomes | 0.0005 | 0.0003 | 0.0011 | 0.0002 | 0.0002 | 0.0003 | 0.0006 | 0.0008 | 0.0004 | 0.0002 | 0.0002 | 0.0003 | 0.0005 | 0.0004 | 0.0014 | 0.0013 | 0.0005 | 0.0005 |
Endoplasmic Reticulum | 0.0055 | 0.0011 | 0.0008 | 0.0006 | 0.001 | 0.0015 | 0.0047 | 0.0015 | 0.004 | 0.0007 | 0.0024 | 0.0011 | 0.0083 | 0.0012 | 0.0016 | 0.0003 | 0.0023 | 0.0007 |
Endosome | 0.1026 | 0.0704 | 0.0681 | 0.0777 | 0.0714 | 0.0592 | 0.1058 | 0.0819 | 0.0806 | 0.0472 | 0.0872 | 0.0444 | 0.0957 | 0.0921 | 0.0567 | 0.0258 | 0.0457 | 0.0481 |
Golgi | 0.074 | 0.081 | 0.0754 | 0.0609 | 0.0502 | 0.0663 | 0.0997 | 0.1473 | 0.1222 | 0.0631 | 0.1398 | 0.108 | 0.1043 | 0.1065 | 0.0731 | 0.0603 | 0.1255 | 0.0904 |
Lipid Particles | 0.0339 | 0.0058 | 0.0061 | 0.0065 | 0.0174 | 0.0139 | 0.032 | 0.0037 | 0.017 | 0.0016 | 0.0102 | 0.0057 | 0.0187 | 0.0112 | 0.0044 | 0.0024 | 0.0136 | 0.0109 |
Mitochondria | 0.6642 | 0.7443 | 0.7594 | 0.772 | 0.7254 | 0.7723 | 0.5666 | 0.5615 | 0.6618 | 0.8622 | 0.6169 | 0.7775 | 0.6018 | 0.6388 | 0.7575 | 0.8718 | 0.68 | 0.7447 |
None | 0.0021 | 0.0025 | 0.0079 | 0.0035 | 0.0087 | 0.0014 | 0.0018 | 0.0006 | 0.0013 | 0.0003 | 0.0025 | 0.0002 | 0.0102 | 0.0017 | 0.007 | 0.0007 | 0.0068 | 0.0007 |
Nuclear Periphery | 0.0065 | 0.0003 | 0.0003 | 0.0003 | 0.0055 | 0.0005 | 0.0257 | 0.0003 | 0.0039 | 0.0004 | 0.0123 | 0.0004 | 0.0091 | 0.0006 | 0.0059 | 0.0001 | 0.0034 | 0.0045 |
Nucleolus | 0.0026 | 0.0003 | 0.0003 | 0.0003 | 0.0033 | 0.0003 | 0.0011 | 0.0003 | 0.0003 | 0.0002 | 0.0007 | 0.0002 | 0.0013 | 0.0003 | 0.0004 | 0.0001 | 0.0005 | 0.0001 |
Nucleus | 0.0011 | 0.0004 | 0.0004 | 0.0004 | 0.0069 | 0.0004 | 0.0299 | 0.0004 | 0.0015 | 0.0004 | 0.0062 | 0.0002 | 0.0024 | 0.0004 | 0.0017 | 0.0001 | 0.0008 | 0.0003 |
Peroxisomes | 0.0261 | 0.0198 | 0.0221 | 0.0162 | 0.0196 | 0.0286 | 0.0129 | 0.0204 | 0.0173 | 0.0022 | 0.0194 | 0.009 | 0.0366 | 0.0333 | 0.0265 | 0.0044 | 0.0288 | 0.0426 |
Punctate Nuclear | 0.0008 | 0.0002 | 0.0002 | 0.0004 | 0.0056 | 0.0003 | 0.0047 | 0.0002 | 0.0006 | 0.0001 | 0.0249 | 0.0001 | 0.0023 | 0.0002 | 0.0015 | 0 | 0.0007 | 0.0002 |
Vacuole | 0.0135 | 0.0045 | 0.0056 | 0.008 | 0.0072 | 0.0059 | 0.0093 | 0.0068 | 0.0047 | 0.0039 | 0.0027 | 0.0037 | 0.008 | 0.0137 | 0.0037 | 0.0009 | 0.0036 | 0.0014 |
Vacuole Periphery | 0.0127 | 0.0028 | 0.0044 | 0.0093 | 0.006 | 0.0062 | 0.0107 | 0.0044 | 0.0051 | 0.0037 | 0.0038 | 0.0026 | 0.0062 | 0.0066 | 0.0036 | 0.0014 | 0.0054 | 0.0026 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 2.6272 | 3.1314 | 2.3236 | 5.586 | 2.2708 | 8.7523 | 5.1449 | 5.8765 | 4.7407 | 3.3521 |
Translational Efficiency | – | – | – | – | – | – | – | – | – | – |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | – |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
340 | 1350 | 844 | 2256 | 1707 | 2347 | 152 | 1496 | 2047 | 3697 | 996 | 3752 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 742.80 | 908.77 | 1042.40 | 981.44 | 760.65 | 881.01 | 1108.57 | 1056.61 | 757.69 | 891.15 | 1052.50 | 1011.41 |
Standard Deviation | 94.63 | 139.25 | 146.67 | 141.34 | 103.45 | 122.52 | 123.19 | 148.59 | 102.25 | 129.57 | 145.30 | 148.90 |
Intensity Change Log 2 | — | 0.290941 | 0.488863 | 0.401926 | — | 0.211926 | 0.543395 | 0.474138 | — | 0.251505 | 0.516711 | 0.438913 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.001450 | 0.003821 | 0.003926 | 0.016167 | 0.001010 | 0.004797 | 0.012537 | 0.019233 | 0.001083 | 0.004440 | 0.005240 | 0.017390 |
Bud Neck | 0.001777 | 0.003398 | 0.006766 | 0.001457 | 0.002946 | 0.003017 | 0.010333 | 0.002394 | 0.002752 | 0.003156 | 0.007310 | 0.001831 |
Bud Site | 0.007322 | 0.028517 | 0.042559 | 0.048576 | 0.013875 | 0.017712 | 0.065458 | 0.083951 | 0.012787 | 0.021658 | 0.046053 | 0.062681 |
Cell Periphery | 0.001697 | 0.000431 | 0.000232 | 0.001269 | 0.000471 | 0.000433 | 0.001586 | 0.001298 | 0.000675 | 0.000432 | 0.000439 | 0.001281 |
Cytoplasm | 0.001950 | 0.008412 | 0.015655 | 0.018657 | 0.004356 | 0.006046 | 0.013595 | 0.022167 | 0.003956 | 0.006910 | 0.015341 | 0.020057 |
Cytoplasmic Foci | 0.016363 | 0.064085 | 0.147268 | 0.039570 | 0.038267 | 0.042823 | 0.131790 | 0.069518 | 0.034629 | 0.050587 | 0.144906 | 0.051511 |
Eisosomes | 0.000057 | 0.000088 | 0.000061 | 0.000150 | 0.000071 | 0.000098 | 0.000110 | 0.000155 | 0.000069 | 0.000095 | 0.000068 | 0.000152 |
Endoplasmic Reticulum | 0.000763 | 0.000661 | 0.002951 | 0.000878 | 0.000378 | 0.000276 | 0.003232 | 0.001280 | 0.000442 | 0.000416 | 0.002994 | 0.001038 |
Endosome | 0.032811 | 0.090264 | 0.158581 | 0.053676 | 0.077721 | 0.079346 | 0.168236 | 0.078052 | 0.070261 | 0.083333 | 0.160055 | 0.063395 |
Golgi | 0.036318 | 0.138692* | 0.182875* | 0.149121* | 0.099985 | 0.131383* | 0.129596* | 0.189674* | 0.089410 | 0.134052* | 0.174744* | 0.165290* |
Lipid Particles | 0.002255 | 0.001351 | 0.002665 | 0.000220 | 0.001850 | 0.000807 | 0.002142 | 0.000248 | 0.001917 | 0.001006 | 0.002586 | 0.000231 |
Mitochondria | 0.851953* | 0.573357* | 0.116117* | 0.592918* | 0.691511* | 0.647872* | 0.179184* | 0.409368* | 0.718160* | 0.620662* | 0.125742* | 0.519733* |
Mitotic Spindle | 0.003520 | 0.017161 | 0.098973 | 0.020769 | 0.006568 | 0.011378 | 0.090180 | 0.037673 | 0.006062 | 0.013490 | 0.097631 | 0.027509 |
None | 0.000237 | 0.000246 | 0.000349 | 0.000266 | 0.000260 | 0.000184 | 0.000182 | 0.000308 | 0.000256 | 0.000206 | 0.000323 | 0.000283 |
Nuclear Periphery | 0.000117 | 0.000163 | 0.000983 | 0.000051 | 0.000058 | 0.000032 | 0.000258 | 0.000118 | 0.000068 | 0.000080 | 0.000873 | 0.000078 |
Nuclear Periphery Foci | 0.000075 | 0.000305 | 0.002501 | 0.000212 | 0.000150 | 0.000074 | 0.000982 | 0.000217 | 0.000137 | 0.000158 | 0.002269 | 0.000214 |
Nucleolus | 0.000172 | 0.000179 | 0.000906 | 0.000016 | 0.000164 | 0.000087 | 0.000455 | 0.000065 | 0.000166 | 0.000121 | 0.000837 | 0.000036 |
Nucleus | 0.002278 | 0.000277 | 0.003344 | 0.000288 | 0.000414 | 0.000436 | 0.001553 | 0.000146 | 0.000724 | 0.000378 | 0.003070 | 0.000231 |
Peroxisomes | 0.020933 | 0.037074 | 0.021739 | 0.027043 | 0.033435 | 0.036752 | 0.073005 | 0.032414 | 0.031358 | 0.036869 | 0.029562 | 0.029184 |
Vacuole | 0.004279 | 0.015472 | 0.124283 | 0.013735 | 0.011498 | 0.008800 | 0.071457 | 0.024815 | 0.010299 | 0.011236 | 0.116222 | 0.018153 |
Vacuole Periphery | 0.013675 | 0.016046 | 0.067266 | 0.014959 | 0.015012 | 0.007649 | 0.044129 | 0.026905 | 0.014790 | 0.010715 | 0.063735 | 0.019722 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -3.69 | -2.50 | -9.92 | -8.19 | -7.22 | -5.73 | -2.22 | -9.43 | -7.10 | -1.26 | -7.17 | -3.74 | -14.07 | -10.48 | -7.63 |
Bud Neck | -2.02 | -4.95 | 0.71 | 3.57 | 6.02 | -0.13 | -1.90 | 1.07 | 1.33 | 2.04 | -0.79 | -4.67 | 2.28 | 3.86 | 5.85 |
Bud Site | -5.64 | -8.91 | -11.42 | -5.94 | -1.34 | -1.91 | -4.74 | -15.99 | -15.06 | -1.65 | -4.88 | -10.44 | -19.75 | -16.17 | -4.27 |
Cell Periphery | 2.07 | 2.40 | 0.65 | -3.00 | -3.72 | 0.42 | -1.30 | -3.84 | -4.04 | 0.31 | 1.97 | 1.39 | -2.75 | -4.46 | -3.74 |
Cytoplasm | -4.14 | -6.80 | -10.42 | -6.71 | -0.84 | -1.90 | -2.92 | -11.41 | -10.12 | -1.81 | -3.81 | -7.21 | -15.54 | -12.44 | -1.83 |
Cytoplasmic Foci | -12.91 | -22.78 | -8.09 | 7.78 | 19.87 | -1.90 | -8.14 | -10.06 | -9.04 | 5.34 | -7.53 | -22.06 | -8.06 | -0.45 | 18.88 |
Eisosomes | -4.06 | -0.61 | -9.81 | -8.08 | -11.60 | -5.66 | -2.52 | -10.86 | -7.15 | -2.63 | -6.63 | -0.00 | -14.70 | -10.27 | -13.22 |
Endoplasmic Reticulum | 0.47 | -5.15 | -0.51 | -1.38 | 5.19 | 1.49 | -1.59 | -5.58 | -6.53 | 1.09 | 0.36 | -6.05 | -5.20 | -5.83 | 4.63 |
Endosome | -11.01 | -18.94 | -4.83 | 9.29 | 18.48 | -0.41 | -6.77 | -0.08 | 0.38 | 6.81 | -3.79 | -15.72 | 2.18 | 7.70 | 18.60 |
Golgi | -14.88 | -16.49 | -17.59 | -1.56 | 3.57 | -5.88 | -2.06 | -13.13 | -8.58 | -4.50 | -10.00 | -11.27 | -15.85 | -6.82 | 0.81 |
Lipid Particles | 0.83 | -0.45 | 1.90 | 4.41 | 5.06 | 3.26 | -0.67 | 5.44 | 3.92 | 3.75 | 2.83 | -1.46 | 5.55 | 5.85 | 5.68 |
Mitochondria | 16.92 | 50.32* | 17.28 | -1.48 | -47.04* | 4.06 | 22.21 | 23.01 | 20.53 | -9.83 | 10.52 | 60.53* | 21.25 | 12.17 | -43.87* |
Mitotic Spindle | -6.71 | -13.88 | -10.58 | -1.75 | 11.26 | -4.33 | -5.27 | -11.88 | -9.77 | 3.23 | -7.13 | -14.58 | -15.14 | -9.03 | 10.99 |
None | -0.04 | -0.93 | -0.57 | -0.88 | 0.79 | 2.16 | 1.70 | -1.21 | -5.02 | -3.13 | 1.74 | -0.68 | -0.77 | -4.81 | 0.47 |
Nuclear Periphery | -0.44 | -3.97 | 0.86 | 1.62 | 4.51 | 2.46 | -4.03 | -2.40 | -3.60 | 2.61 | -0.42 | -4.59 | -0.61 | 0.03 | 4.53 |
Nuclear Periphery Foci | -2.24 | -4.82 | -1.22 | 0.58 | 4.48 | 2.47 | -3.73 | -1.15 | -3.83 | 3.50 | -0.21 | -4.98 | -0.94 | -0.77 | 4.79 |
Nucleolus | 0.12 | -2.48 | 1.55 | 1.95 | 3.08 | 1.06 | -0.75 | 1.21 | 0.48 | 1.30 | 0.90 | -2.42 | 1.62 | 2.14 | 3.22 |
Nucleus | 1.32 | -0.98 | 1.12 | -0.67 | 3.93 | 0.09 | -2.06 | 1.53 | 0.80 | 2.50 | 1.09 | -3.90 | 1.01 | -0.05 | 4.56 |
Peroxisomes | -2.98 | -0.23 | -1.23 | 2.88 | -1.66 | -0.95 | -3.29 | 0.34 | 1.31 | 3.39 | -1.91 | 0.49 | 0.86 | 3.30 | 0.20 |
Vacuole | -6.67 | -21.29 | -6.81 | 0.93 | 19.77 | 2.41 | -6.55 | -7.62 | -9.62 | 5.16 | -1.01 | -21.37 | -7.36 | -7.10 | 19.73 |
Vacuole Periphery | -1.06 | -11.75 | -0.59 | 0.71 | 12.28 | 7.21 | -3.87 | -5.79 | -10.19 | 2.20 | 4.16 | -12.91 | -3.92 | -8.31 | 11.49 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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