Standard name
Human Ortholog
Description FG-nucleoporin component of central core of the nuclear pore complex; also part of the nuclear pore complex (NPC) cytoplasmic filaments; contributes directly to nucleocytoplasmic transport; regulates ADP release from the ATP-dependent RNA helicase Dbp5p; forms a stable association with Nup82p, Gle2p and two other FG-nucleoporins (Nsp1p and Nup116p)
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 – 0 0 0 0 0.06 0.07 0.07 0.09 0.06 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0.06 0.06 0.13 0 0 0.05 – 0 0 0 0.05 0.06 0.09 0.05 0.07 0.08 0 0 0 0 0 0.06
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.05 0.11 0.18 0.28 0.3 0.52 0.48 0.42 – 0.59 0.63 0.55 0 0 0 0.07 0.09 0.08 0 0 0 0.08 0.15 0.19
Nucleus 0 0 0 0.05 0 0 0 0 – 0 0 0 0 0 0 0 0.11 0 0 0 0 0 0 0
Nuclear Periphery 0.81 0.75 0.81 0.66 0.55 0.53 0.48 0.46 – 0.45 0.49 0.57 0.77 0.7 0.62 0.54 0.49 0.61 0.89 0.84 0.88 0.79 0.68 0.59
Nucleolus 0.09 0.07 0.16 0.29 0.28 0.33 0.34 0.35 – 0.3 0.33 0.35 0.15 0.14 0.18 0.11 0.11 0.16 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.09 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.42 0.34 0.18 0.1 0.11 0.05 0.06 0.07 – 0.05 0.06 0 0.27 0.22 0.22 0.39 0.36 0.36 0 0.07 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0 4 1 1 0 1 1 2 4 3 6 11
Bud 0 2 1 0 0 2 2 3 – 7 4 7 0 0 0 0 4 0 6 2 4 2 3 1
Bud Neck 0 0 0 0 0 0 0 1 – 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 1 2 1 – 13 14 9 1 0 1 0 1 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 6 2 9 14 16 10 7 8 – 5 9 3 7 15 13 45 43 23 0 0 0 0 0 0
Endoplasmic Reticulum 0 1 3 1 2 2 2 5 – 2 5 0 0 0 1 19 17 8 2 1 1 1 2 2
Endosome 5 0 35 22 57 26 32 56 – 6 10 16 9 15 15 30 34 29 12 10 13 11 16 29
Golgi 3 0 2 0 0 0 0 1 – 0 0 0 0 0 1 5 1 4 3 3 3 7 11 19
Mitochondria 34 54 107 112 133 372 408 428 – 546 488 545 4 6 1 46 46 28 13 11 22 33 59 95
Nucleus 10 10 4 18 8 12 23 33 – 25 23 23 3 3 0 27 54 14 2 4 1 2 0 3
Nuclear Periphery 504 376 477 261 248 380 414 472 – 414 377 559 129 171 108 342 247 227 600 466 550 320 275 289
Nucleolus 54 36 94 113 127 236 290 358 – 274 252 343 25 35 32 68 56 59 5 7 1 3 2 3
Peroxisomes 1 0 2 0 2 1 5 3 – 2 0 5 0 5 10 1 0 2 0 0 1 5 8 11
SpindlePole 0 0 4 1 2 9 10 12 – 5 6 6 1 8 16 4 10 2 2 2 3 2 1 3
Vac/Vac Membrane 263 169 107 40 49 38 51 69 – 51 43 38 45 55 38 247 179 136 18 39 10 10 12 18
Unique Cell Count 624 499 586 396 449 713 856 1031 928 770 984 168 246 174 635 502 373 673 553 623 406 402 493
Labelled Cell Count 880 650 845 582 644 1089 1246 1450 1350 1232 1555 224 317 237 835 692 533 673 553 623 406 402 493


Nuclear Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 14.1 13.2 11.0 9.3 9.5 7.9 7.8 8.7 – 7.7 7.5 7.7 12.8 12.2 13.1 9.6 9.6 11.9 13.1 14.0 14.8
Std Deviation (1e-4) 2.8 3.0 2.7 2.7 2.5 2.2 2.2 2.6 – 2.3 2.5 2.2 3.2 3.3 4.7 2.4 3.1 3.4 3.0 3.1 3.1
Intensity Change (Log2) – – – -0.24 -0.21 -0.46 -0.49 -0.33 – -0.51 -0.54 -0.5 0.23 0.16 0.26 -0.19 -0.19 0.12 0.26 0.35 0.44


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 2.9 3.3 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cytoplasm 2.0 2.1 -0.2 -1.3 -1.4 – -2.0 -0.6 0 0 3.6 4.1 4.7 5.4 3.9
Endoplasmic Reticulum 0 0 0 0 0 – 0 0 0 0 0 0 3.3 3.5 0
Endosome -0.3 3.8 -2.0 -2.0 -0.5 – -6.2 -4.8 -4.7 -0.3 0.1 1.2 -1.0 0.5 1.1
Golgi 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Mitochondria 3.7 4.3 12.6 11.4 9.6 – 15.5 16.6 14.4 -5.1 -6.1 -5.9 -5.8 -4.3 -4.7
Nucleus 4.0 1.6 1.6 2.8 3.3 – 2.8 3.0 2.4 0 0 0 4.0 7.4 3.4
Nuclear Periphery -5.5 -9.1 -10.6 -12.7 -14.0 – -14.2 -12.3 -9.9 -1.3 -3.8 -5.3 -10.2 -11.2 -7.0
Nucleolus 4.7 4.8 7.0 7.5 8.0 – 6.0 7.0 8.0 -0.4 -0.7 0.7 -2.7 -2.3 -0.1
Peroxisomes 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 1.0 0.9 0.9 – 0 0 0 0 0 0 0 1.9 0
Vacuole -3.5 -3.3 -7.4 -7.3 -7.2 – -7.9 -7.4 -9.5 2.4 1.4 1.1 7.9 6.5 6.3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 9.1221 8.4975 9.8258 8.2101 8.6629 9.3397 10.6812 12.2598 11.3096 10.3405 10.2216 11.4702 11.842 12.608 11.6746 11.784 10.7505 11.8339
Actin 0.0528 0.0012 0.0007 0.0314 0.0041 0.0059 0.0201 0.001 0.012 0.0099 0.0037 0.0035 0.0036 0.0003 0.0058 0.0082 0.0076 0.0001
Bud 0.0005 0.0002 0 0.0003 0.0001 0.0001 0.0003 0.0007 0.0004 0 0.0001 0.0001 0.0001 0 0.0001 0.0001 0.0003 0
Bud Neck 0.0008 0.0001 0 0.0005 0.0014 0.0001 0.006 0.0001 0.0008 0.0003 0.0002 0.0001 0.0001 0 0.001 0.0002 0.0007 0
Bud Periphery 0.001 0.0003 0.0001 0.0012 0.0002 0.0001 0.0004 0.0005 0.0007 0.0002 0.0004 0.0001 0.0001 0.0002 0.0002 0.0007 0.0008 0
Bud Site 0.0008 0.001 0 0.0006 0.0003 0.0001 0.0038 0.0006 0.003 0.0001 0.0004 0.0001 0.0005 0 0.0004 0.0001 0.0011 0
Cell Periphery 0.0002 0.0002 0 0.0002 0.0001 0 0.0002 0.0001 0.0003 0.0001 0.0001 0 0.0001 0 0.0001 0.0002 0.0003 0
Cytoplasm 0.0164 0.0076 0.0003 0.001 0.0113 0.0003 0.0097 0.012 0.0008 0.0002 0.0016 0.0006 0.0131 0.0001 0.0007 0.0001 0.0035 0
Cytoplasmic Foci 0.002 0.0006 0 0.0053 0.0069 0.0004 0.0132 0.0001 0.0064 0.0086 0.004 0.0006 0.0093 0 0.0011 0.0002 0.0064 0
Eisosomes 0.0005 0 0 0.0004 0.0001 0 0.0001 0.0001 0.0002 0.0001 0.0001 0.0001 0.0002 0 0.0002 0.0003 0.0002 0
Endoplasmic Reticulum 0.0054 0.0023 0.0017 0.0078 0.0018 0.0003 0.0018 0.0023 0.0023 0.0042 0.0028 0.001 0.0052 0.0017 0.002 0.0066 0.01 0.0004
Endosome 0.0132 0.0035 0.0005 0.0097 0.0065 0.0022 0.0121 0.0008 0.0158 0.0073 0.0243 0.0042 0.012 0.0007 0.0011 0.0026 0.0196 0.0001
Golgi 0.0096 0.0007 0.0001 0.004 0.001 0.0037 0.0044 0.0002 0.0041 0.0029 0.0038 0.0016 0.0016 0.0002 0.0007 0.0023 0.0034 0.0001
Lipid Particles 0.0119 0.0073 0.0003 0.0498 0.0021 0.0018 0.0227 0.0017 0.0047 0.0209 0.0207 0.0017 0.0084 0.0017 0.0031 0.0071 0.0334 0.0008
Mitochondria 0.0174 0.0024 0.002 0.0087 0.0027 0.0019 0.0025 0.0083 0.007 0.0084 0.0061 0.0062 0.0016 0.0141 0.0037 0.0088 0.0051 0.0015
None 0.0017 0.0005 0.0002 0.0016 0.0252 0.0001 0.0062 0.0009 0.0005 0.0005 0.002 0.0009 0.0235 0.0002 0.0033 0.0007 0.002 0.0001
Nuclear Periphery 0.7608 0.7103 0.8822 0.7608 0.7809 0.9178 0.81 0.9053 0.8635 0.8355 0.8654 0.9558 0.8667 0.9023 0.9114 0.8955 0.8281 0.9735
Nucleolus 0.0026 0.0064 0.0004 0.0153 0.0028 0.0021 0.0055 0.0004 0.0035 0.001 0.0058 0.0002 0.0034 0.0002 0.0003 0.0008 0.0045 0.0002
Nucleus 0.0608 0.1881 0.0665 0.0488 0.1018 0.0446 0.0065 0.014 0.0147 0.0068 0.0093 0.0023 0.0113 0.0176 0.0047 0.005 0.0176 0.0013
Peroxisomes 0.0024 0.0002 0 0.0004 0.0003 0.0001 0.006 0 0.0021 0.028 0.0013 0.0012 0.0055 0 0.0004 0.0002 0.0005 0
Punctate Nuclear 0.0022 0.0026 0.0006 0.0117 0.0171 0.0009 0.0272 0.0005 0.0012 0.0029 0.0129 0.0012 0.0029 0.0004 0.0009 0.0015 0.016 0.0002
Vacuole 0.0053 0.0246 0.0007 0.0023 0.0026 0.0008 0.0024 0.0015 0.0107 0.0029 0.002 0.0002 0.0022 0.0015 0.0009 0.0017 0.0056 0.0001
Vacuole Periphery 0.0318 0.0398 0.0436 0.0384 0.0308 0.0167 0.0388 0.049 0.0453 0.0592 0.0331 0.0183 0.0286 0.0585 0.0578 0.0569 0.0334 0.0214

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 47.9577 39.6081 48.0942 55.0276 46.8384 46.0441 48.9219 50.9874 49.1416 53.7104
Translational Efficiency 1.3809 1.3809 1.2383 1.1008 1.2746 1.1891 0.9901 0.9516 1.0254 1.0029

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description FG-nucleoporin component of central core of the nuclear pore complex; also part of the nuclear pore complex (NPC) cytoplasmic filaments; contributes directly to nucleocytoplasmic transport; regulates ADP release from the ATP-dependent RNA helicase Dbp5p; forms a stable association with Nup82p, Gle2p and two other FG-nucleoporins (Nsp1p and Nup116p)
Localization
Cell Percentages nuclear periphery (90%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Nup159

Nup159


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Nup159-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available