Standard name
Human Ortholog
Description Pheromone-regulated protein, predicted to have 1 transmembrane segment; induced during cell integrity signaling; PRM5 has a paralog, YNL058C, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0 0 0 0 0 – 0 0 0 0 0 – 0 0 0 0 0 – 0 0 0
Bud 0 0 – 0 0 0 0 0 – 0 0 0 0 0 – 0 0 0 0 0 – 0 0 0
Bud Neck 0 0 – 0 0 0 0 0 – 0 0 0 0 0 – 0 0 0 0 0 – 0 0 0
Bud Site 0 0 – 0 0 0 0 0 – 0 0 0 0 0 – 0 0 0 – – – – – –
Cell Periphery 0 0 – 0 0 0 0 0 – 0 0 0 0 0 – 0 0 0 0 0 – 0 0 0
Cytoplasm 0.07 0.11 – 0.09 0.08 0.12 0.17 0.2 – 0.36 0.47 0.52 0.15 0.33 – 0.15 0.2 0.1 0 0 – 0 0 0
Endoplasmic Reticulum 0 0 – 0 0 0 0 0 – 0 0 0 0 0 – 0 0.05 0 0 0 – 0 0 0
Endosome 0 0 – 0 0 0 0 0 – 0 0 0 0.05 0.05 – 0 0 0 0 0 – 0.07 0.06 0
Golgi 0 0 – 0 0 0 0 0 – 0 0 0 0 0 – 0 0 0 0 0 – 0 0 0
Mitochondria 0.09 0.08 – 0.05 0.13 0.11 0.13 0 – 0.07 0.08 0.12 0 0.15 – 0 0 0 0 0 – 0 0 0
Nucleus 0.3 0.21 – 0.37 0.25 0.35 0.35 0.28 – 0.19 0.13 0.13 0.26 0.12 – 0.43 0.38 0.38 0 0 – 0 0 0
Nuclear Periphery 0 0 – 0 0 0 0 0 – 0 0 0 0 0 – 0 0 0 0 0 – 0 0 0
Nucleolus 0 0 – 0 0 0 0 0 – 0 0 0 0 0 – 0 0 0 0 0 – 0 0 0
Peroxisomes 0 0 – 0 0 0 0 0 – 0 0 0 0 0 – 0 0 0 0 0 – 0 0 0
SpindlePole 0 0 – 0 0 0 0 0 – 0 0 0 0 0 – 0 0 0 0 0 – 0 0 0
Vac/Vac Membrane 0.65 0.77 – 0.64 0.71 0.61 0.56 0.64 – 0.61 0.56 0.48 0.67 0.45 – 0.62 0.54 0.68 0.81 0.87 – 0.87 0.84 0.89
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0 1 0 1 0 – 0 0 0 2 1 – 0 0 0 0 1 – 0 0 0
Bud 0 0 – 0 2 3 3 7 – 6 7 8 0 10 – 0 0 0 2 2 – 0 3 1
Bud Neck 0 0 – 0 0 0 0 0 – 0 0 0 0 0 – 0 0 0 0 0 – 0 0 0
Bud Site 0 0 – 0 0 0 0 0 – 0 0 0 0 0 – 0 0 0 – – – – – –
Cell Periphery 0 0 – 0 1 0 0 0 – 0 0 0 0 1 – 0 0 0 0 0 – 0 0 0
Cytoplasm 4 12 – 21 44 82 155 213 – 333 311 457 26 92 – 37 34 18 2 1 – 1 0 0
Endoplasmic Reticulum 0 0 – 0 3 0 1 2 – 1 0 0 1 0 – 6 9 7 0 0 – 1 2 1
Endosome 1 4 – 9 10 5 1 8 – 8 1 5 8 13 – 7 6 3 1 3 – 9 10 6
Golgi 0 0 – 0 0 0 0 0 – 0 0 0 0 0 – 0 1 4 0 1 – 0 1 1
Mitochondria 5 9 – 12 74 75 120 48 – 67 55 101 5 43 – 10 4 1 1 1 – 2 5 7
Nucleus 17 23 – 90 143 239 325 303 – 178 84 112 44 34 – 103 66 67 5 1 – 0 1 2
Nuclear Periphery 0 1 – 1 5 6 24 14 – 6 4 4 0 0 – 2 0 1 2 0 – 0 2 3
Nucleolus 0 0 – 1 4 3 4 7 – 2 1 5 1 2 – 3 1 1 1 0 – 0 1 0
Peroxisomes 0 0 – 0 1 0 0 0 – 1 0 0 0 0 – 0 0 0 0 0 – 0 0 0
SpindlePole 0 0 – 2 2 4 2 3 – 1 0 2 3 7 – 1 1 0 1 0 – 0 0 0
Vac/Vac Membrane 37 85 – 158 399 412 516 698 – 573 366 418 115 127 – 149 93 121 97 111 – 115 162 224
Unique Cell Count 57 111 246 561 679 927 1090 937 658 871 172 280 240 173 177 120 128 133 194 252
Labelled Cell Count 64 134 294 689 829 1152 1303 1176 829 1112 205 330 318 215 223 120 128 133 194 252


Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.0 8.7 – 7.5 7.6 7.4 6.3 7.1 – 6.3 6.2 5.7 7.2 5.1 – 8.1 8.1 8.8 11.7 14.8 17.8
Std Deviation (1e-4) 2.3 1.9 – 2.2 2.0 2.3 2.3 2.2 – 2.1 2.0 2.0 1.7 1.2 – 2.5 2.9 2.8 2.6 3.8 5.0
Intensity Change (Log2) – – – – – – – – – – – – – – – – – – – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.199 5.5434 3.898 3.5316 2.8991 3.959 1.8697 3.5746 2.238 1.7238 2.2985 2.8516 1.2101 3.2363 1.4748 1.2446 1.4387 1.6733
Actin 0.0066 0 0.0058 0.0001 0.0048 0 0.0173 0 0.0018 0 0.0001 0.0003 0.0069 0 0.0022 0 0.0108 0
Bud 0.0016 0.001 0.0006 0.0004 0.0003 0.0004 0.0005 0.0007 0.0007 0.0008 0.0008 0.0005 0.0004 0.0003 0.0006 0.0001 0.0007 0.0004
Bud Neck 0.0011 0.0014 0.0012 0.0011 0.0012 0.0055 0.0014 0.0016 0.0025 0.0019 0.0018 0.0036 0.0013 0.0042 0.0039 0.0005 0.0105 0.0022
Bud Periphery 0.0013 0.0012 0.0012 0.0009 0.0007 0.0017 0.0013 0.0024 0.0039 0.0013 0.0019 0.0023 0.0011 0.001 0.0012 0.0002 0.0013 0.0014
Bud Site 0.0039 0.0043 0.0014 0.0032 0.0011 0.0008 0.0021 0.0017 0.0031 0.0036 0.0025 0.0008 0.0038 0.0016 0.0045 0.0004 0.0126 0.0006
Cell Periphery 0.0041 0.01 0.0042 0.0016 0.0024 0.0074 0.0088 0.0096 0.0054 0.0028 0.0041 0.006 0.0071 0.0093 0.0044 0.0012 0.0044 0.0043
Cytoplasm 0.0095 0.0138 0.0115 0.0025 0.0012 0.0041 0.0235 0.0035 0.0066 0.0036 0.0022 0.0132 0.0424 0.015 0.0047 0.0031 0.0034 0.004
Cytoplasmic Foci 0.0358 0.0089 0.0218 0.0057 0.0193 0.0136 0.0225 0.0052 0.0186 0.0077 0.0056 0.013 0.0187 0.0102 0.0096 0.0104 0.007 0.0063
Eisosomes 0.0001 0 0 0 0 0 0.0002 0 0.0001 0 0 0 0.0001 0 0 0 0.0002 0
Endoplasmic Reticulum 0.0063 0.0068 0.0035 0.0013 0.001 0.0028 0.0074 0.005 0.0041 0.0012 0.002 0.006 0.0102 0.0067 0.0039 0.0011 0.0021 0.0036
Endosome 0.2538 0.1087 0.1861 0.1307 0.1822 0.1266 0.2481 0.1254 0.2207 0.2497 0.1513 0.1659 0.19 0.0914 0.1811 0.2021 0.148 0.1374
Golgi 0.0143 0.003 0.011 0.0042 0.0202 0.0051 0.0124 0.0051 0.013 0.0045 0.0058 0.0071 0.007 0.0029 0.0075 0.0068 0.0071 0.0041
Lipid Particles 0.0339 0.0163 0.0214 0.0057 0.066 0.0129 0.0472 0.0217 0.0402 0.0077 0.0196 0.0212 0.0556 0.0123 0.0223 0.0397 0.0136 0.028
Mitochondria 0.0191 0.0022 0.0331 0.0285 0.0294 0.0141 0.0131 0.0038 0.0674 0.0023 0.0023 0.0144 0.0184 0.0037 0.0318 0.0006 0.0052 0.002
None 0.0101 0.0036 0.0054 0.0008 0.0003 0.0003 0.0212 0.0003 0.0073 0.0003 0.0004 0.0016 0.0233 0.0009 0.0173 0.0006 0.002 0.0002
Nuclear Periphery 0.0047 0.0127 0.0082 0.0025 0.0028 0.0033 0.0083 0.0038 0.009 0.002 0.0033 0.0061 0.0076 0.0077 0.0054 0.0014 0.0031 0.0053
Nucleolus 0.0037 0.0069 0.0056 0.0053 0.003 0.0044 0.0041 0.0034 0.0057 0.0044 0.0055 0.0037 0.0039 0.0043 0.0115 0.0013 0.007 0.0025
Nucleus 0.003 0.0054 0.0036 0.0042 0.0021 0.0094 0.0111 0.0041 0.0104 0.0135 0.0056 0.0056 0.0042 0.0106 0.0045 0.0014 0.0055 0.0086
Peroxisomes 0.0123 0.0004 0.0073 0.0021 0.0132 0.0015 0.0117 0.0007 0.004 0.001 0.0009 0.0009 0.001 0.0005 0.0012 0.0008 0.0014 0.0003
Punctate Nuclear 0.0009 0.0003 0.0012 0.0009 0.0004 0.0004 0.0022 0.0001 0.0005 0.0001 0.001 0.0009 0.0013 0.0001 0.0005 0.0001 0.0075 0.0002
Vacuole 0.4711 0.6848 0.5181 0.6227 0.5413 0.6716 0.4683 0.6786 0.4418 0.6149 0.7072 0.6047 0.52 0.6887 0.5275 0.6421 0.6268 0.6615
Vacuole Periphery 0.1029 0.1081 0.1477 0.1756 0.107 0.114 0.0674 0.1232 0.1335 0.0768 0.0761 0.122 0.0755 0.1286 0.1544 0.0861 0.12 0.1272

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 22.4832 19.5832 9.1779 22.1673 27.6555 25.3516 24.4902 20.0461 22.7636 35.7982
Translational Efficiency 0.9756 1.3191 2.7084 1.2098 1.5907 1.3607 1.357 1.5235 1.6648 1.373

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1506 1607 189 1271 1586 1086 2873 1794 3092 2693 3062 3065

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 727.82 896.84 921.43 851.24 753.99 951.62 778.42 905.64 741.24 918.93 787.25 883.08
Standard Deviation 118.48 138.18 100.70 267.00 136.27 188.45 161.26 262.94 128.58 162.60 161.90 265.98
Intensity Change Log 2 — 0.301269 0.340293 0.225984 — 0.335840 0.046003 0.264392 — 0.318963 0.198047 0.245655

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000066 0.000053 0.001060 0.000615 0.000031 0.000097 0.000293 0.001287 0.000048 0.000071 0.000341 0.001008
Bud Neck 0.015819 0.010783 0.006392 0.020373 0.010806 0.006797 0.023618 0.029566 0.013248 0.009176 0.022555 0.025754
Bud Site 0.010193 0.016039 0.012521 0.057057 0.013265 0.008259 0.027437 0.065641 0.011768 0.012901 0.026516 0.062082
Cell Periphery 0.001371 0.002209 0.000345 0.001008 0.001789 0.008753 0.000970 0.001630 0.001585 0.004848 0.000931 0.001372
Cytoplasm 0.073422 0.031070 0.271738* 0.281315* 0.025322 0.005764 0.517588* 0.249802* 0.048750 0.020865 0.502413* 0.262870*
Cytoplasmic Foci 0.015904 0.005903 0.031803 0.031264 0.008188 0.002677 0.015854 0.036007 0.011946 0.004602 0.016838 0.034040
Eisosomes 0.000031 0.000023 0.000131 0.000057 0.000021 0.000089 0.000036 0.000072 0.000026 0.000050 0.000042 0.000066
Endoplasmic Reticulum 0.002986 0.003838 0.010536 0.002980 0.003499 0.004940 0.001389 0.002303 0.003249 0.004282 0.001953 0.002584
Endosome 0.035658 0.031567 0.025344 0.032600 0.045662 0.021377 0.005985 0.050400 0.040790 0.027457 0.007180 0.043019
Golgi 0.000821 0.002355 0.000433 0.006539 0.001862 0.001698 0.000931 0.005243 0.001355 0.002090 0.000901 0.005780
Lipid Particles 0.001356 0.000565 0.007451 0.001849 0.000867 0.000669 0.000962 0.002577 0.001105 0.000607 0.001362 0.002275
Mitochondria 0.000544 0.001368 0.000490 0.004401 0.001717 0.004184 0.001976 0.002198 0.001145 0.002504 0.001884 0.003112
Mitotic Spindle 0.000165 0.000200 0.002272 0.022680 0.000074 0.000491 0.019093 0.011447 0.000118 0.000317 0.018055 0.016105
None 0.000472 0.000409 0.001402 0.003179 0.000865 0.000432 0.004992 0.002734 0.000673 0.000419 0.004771 0.002918
Nuclear Periphery 0.000226 0.000302 0.002123 0.001507 0.000264 0.000285 0.001455 0.000753 0.000246 0.000295 0.001496 0.001066
Nuclear Periphery Foci 0.000228 0.000389 0.012621 0.001833 0.000360 0.000748 0.000567 0.001186 0.000295 0.000534 0.001311 0.001454
Nucleolus 0.000749 0.000936 0.001193 0.002472 0.001747 0.004334 0.001241 0.001562 0.001261 0.002306 0.001238 0.001939
Nucleus 0.014241 0.004725 0.276287* 0.089034 0.006603 0.010601 0.235845* 0.079381 0.010323 0.007095 0.238341* 0.083384
Peroxisomes 0.000537 0.000472 0.002722 0.003662 0.000745 0.000710 0.001250 0.003850 0.000644 0.000568 0.001340 0.003772
Vacuole 0.810972* 0.813036* 0.331689* 0.412894* 0.811941* 0.876543* 0.129815 0.434129* 0.811469* 0.838646* 0.142276 0.425323*
Vacuole Periphery 0.014239 0.073759 0.001446 0.022682 0.064372 0.040552 0.008704 0.018231 0.039954 0.060367 0.008256 0.020077

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 0.37 -8.43 -3.79 -4.07 3.12 -3.16 -3.94 -3.26 -3.05 -2.64 -1.14 -4.55 -4.08 -3.98 -2.88
Bud Neck 3.32 6.81 -2.37 -5.69 -8.97 3.08 -7.34 -8.63 -10.94 -1.95 4.02 -5.85 -8.19 -11.62 -1.31
Bud Site -3.41 -0.27 -10.14 -8.37 -9.39 2.69 -3.53 -12.86 -14.19 -10.55 -0.93 -4.76 -16.52 -15.75 -12.22
Cell Periphery -2.19 4.26 1.60 3.51 -1.58 -6.11 6.71 1.43 6.36 -2.89 -6.38 5.73 1.96 7.06 -2.50
Cytoplasm 7.92 -10.00 -23.81 -30.50 -2.37 6.53 -74.91* -34.55 -38.98 24.06 8.73 -70.01* -40.26 -47.44 24.63
Cytoplasmic Foci 6.73 -3.16 -5.81 -11.97 -0.32 4.77 -5.62 -14.31 -18.19 -11.18 7.76 -3.42 -14.21 -21.15 -11.82
Eisosomes 2.19 -10.75 -5.77 -8.48 8.09 -6.24 -6.60 -11.33 1.92 -7.99 -4.90 -6.89 -12.00 -2.49 -7.06
Endoplasmic Reticulum -2.11 -9.36 0.78 2.30 9.08 -2.68 5.71 3.63 5.95 -2.69 -3.15 4.52 3.07 5.86 -0.45
Endosome 1.62 4.12 3.52 2.20 -1.64 7.59 17.15 0.55 -6.85 -15.70 6.60 21.75 1.92 -4.33 -18.16
Golgi -2.45 1.49 -4.23 -2.25 -4.97 0.23 3.77 -3.07 -2.82 -4.70 -1.70 2.76 -5.15 -3.41 -6.41
Lipid Particles 3.88 -6.58 -1.74 -5.88 6.04 0.79 -1.22 -4.97 -6.25 -5.00 3.23 -2.40 -5.07 -8.50 -3.63
Mitochondria -2.07 0.15 -3.90 -2.58 -3.91 -1.80 0.03 -0.73 1.60 -1.05 -2.23 -1.99 -3.86 -0.21 -2.68
Mitotic Spindle -0.31 -1.98 -8.71 -8.70 -7.16 -1.60 -10.85 -7.14 -6.58 2.12 -1.68 -10.89 -11.22 -10.96 -0.34
None 0.41 -3.50 -4.68 -4.86 -2.42 1.19 -7.61 -2.71 -3.70 2.87 1.34 -10.04 -4.85 -5.86 3.16
Nuclear Periphery -0.77 -14.62 -6.76 -5.95 2.98 -0.23 -16.81 -4.61 -4.89 9.03 -0.68 -21.90 -8.24 -7.39 7.04
Nuclear Periphery Foci -1.42 -7.66 -3.18 -2.78 6.66 -1.90 -2.36 -2.88 -1.28 -1.97 -2.20 -7.46 -4.34 -3.17 0.09
Nucleolus -1.21 -1.33 -2.71 -2.34 -0.52 -2.87 0.93 0.61 3.36 -0.38 -2.66 -0.49 -1.51 1.35 -1.28
Nucleus 6.30 -13.98 -18.46 -21.43 9.23 -1.84 -57.81 -19.81 -17.17 32.95 2.65 -58.95 -26.74 -27.87 35.50
Peroxisomes 0.85 -2.73 -4.11 -4.23 -1.44 -0.05 -0.96 -6.91 -6.53 -5.96 0.62 -1.92 -7.83 -7.92 -6.05
Vacuole -0.30 20.96 34.50 36.80* -2.59 -7.45 93.83* 39.85* 45.46* -33.65 -4.42 108.56* 52.59* 57.40* -37.75
Vacuole Periphery -18.04* 12.54 -1.11 15.61 -8.12 6.24 20.41 16.02 8.45 -5.88 -7.61 19.71 12.65 18.14 -7.51
Standard name
Human Ortholog
Description Pheromone-regulated protein, predicted to have 1 transmembrane segment; induced during cell integrity signaling; PRM5 has a paralog, YNL058C, that arose from the whole genome duplication
Localization
Cell Percentages vacuole (82%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Prm5

Prm5


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Prm5-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available