Standard name
Human Ortholog
Description Transcription factor, allelic differences between S288C and Sigma1278b; mediates fermentation stress tolerance by modulating cell wall integrity; overexpression suppresses heat shock sensitivity of wild-type RAS2 overexpression and also suppresses cell lysis defect of mpk1 mutation; allele from S288c can confer fMAPK pathway independent transcription of FLO11; S288C and Sigma1278b alleles differ in number of tandem repeats within ORF

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0.07 0 0 0 0.05 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.65 0.56 0.83 0.61 0.69 0.42 0.18 0.25 0.3 0.2 0.22 0.25 0.83 0.77 0.8 0.89 0.93 0.85 0.45 0.42 0.43 0.63 0.64 0.66
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.06 0.08 0 0 0 0 0 0
Endosome 0 0 0 0.05 0.06 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0.05 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.32 0.19 0.1 0.44 0.26 0.66 0.83 0.75 0.7 0.79 0.83 0.83 0 0 0 0.06 0 0.07 0.14 0.08 0.24 0.07 0.07 0.08
Nucleus 0 0.07 0 0.05 0 0.05 0.11 0.13 0.15 0.14 0.14 0.09 0.05 0.06 0.07 0 0 0 0.06 0.16 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0.07 0.05 0.05 0.06
Vac/Vac Membrane 0.11 0.25 0.07 0.05 0.08 0.11 0.1 0 0 0.06 0 0 0.09 0.16 0.12 0 0.05 0.07 0.15 0.18 0.08 0.12 0.12 0.08
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 1 0 0 0 1 0 0 0 0 0 0 0 0 0 0 2 3 2 1 2 1 6
Bud 2 1 0 0 0 1 12 4 7 8 10 14 1 0 0 0 0 2 2 3 1 1 6 5
Bud Neck 0 0 0 0 0 0 0 0 0 1 0 0 2 5 3 1 1 2 0 1 0 3 2 6
Bud Site 0 0 1 0 0 0 3 4 5 6 6 10 0 0 0 0 0 0
Cell Periphery 0 0 0 0 1 0 0 0 0 0 0 0 0 0 1 1 1 1 0 0 0 1 1 3
Cytoplasm 183 63 71 37 94 51 31 56 61 40 40 60 231 297 339 174 166 124 158 65 49 163 233 300
Endoplasmic Reticulum 2 4 3 0 1 0 1 1 0 0 0 0 2 3 1 13 11 11 5 0 4 1 1 2
Endosome 1 2 2 3 8 0 0 2 0 0 0 0 6 18 16 1 1 5 10 4 5 2 5 9
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 1 1 0 0 0 0 0
Mitochondria 91 21 9 27 36 81 144 167 141 154 151 196 3 2 4 12 5 10 47 12 28 18 25 36
Nucleus 8 8 1 3 3 6 20 29 31 28 25 22 14 23 30 3 3 1 21 24 0 8 13 9
Nuclear Periphery 0 1 0 0 0 1 1 2 0 1 0 4 0 0 0 0 0 0 1 0 0 0 0 0
Nucleolus 0 0 0 0 1 1 2 4 2 2 3 3 0 0 0 0 0 0 2 0 0 0 0 1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 1 1 0 0 2 0 3 1 2 2 2 5 5 3 0 2 21 4 7 12 17 25
Vac/Vac Membrane 32 28 6 3 11 13 17 9 9 12 4 4 26 64 51 6 9 10 52 27 9 32 45 35
Unique Cell Count 282 113 86 61 136 122 174 223 202 196 182 237 278 388 423 196 179 146 349 155 116 260 365 456
Labelled Cell Count 320 128 95 74 155 154 234 278 259 253 241 315 287 417 452 215 197 171 349 155 116 260 365 456


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.3 4.5 4.1 3.7 4.2 3.2 3.4 3.2 4.6 3.1 2.7 2.7 5.2 5.2 5.1 5.0 5.1 5.0 5.3 5.5 5.7
Std Deviation (1e-4) 0.4 1.2 1.1 1.4 2.3 1.2 1.0 1.5 10.0 1.1 0.8 0.8 1.1 1.0 1.1 1.3 1.7 1.2 1.1 1.2 1.2
Intensity Change (Log2) -0.12 0.04 -0.36 -0.28 -0.33 0.16 -0.42 -0.62 -0.62 0.35 0.36 0.32 0.29 0.33 0.28 0.37 0.43 0.48


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud 0
Bud Neck 0
Bud Site 0
Cell Periphery 0
Cyto
Endoplasmic Reticulum 0
Endosome 0
Golgi 0
Mitochondria 0
Nuclear Periphery 0
Nuc
Nucleolus 0
Peroxisomes 0
SpindlePole 0
Vac
Cortical Patches 0
Cytoplasm 0
Nucleus 0
Vacuole 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.6164 1.7967 1.4809 1.7787 1.6167 1.8494 0.9567 0.253 0.1308 0.1542 0.6119 0.1156 -0.4481 -0.3194 -0.4985 -0.4682 -0.3393 -0.326
Actin 0.0095 0.001 0.0231 0.0153 0.0464 0.0024 0.0215 0.0045 0.0204 0.0915 0.0487 0.0015 0.0223 0.0002 0.0023 0.0005 0.0024 0.002
Bud 0.0055 0.0011 0.0005 0.0006 0.0003 0.0001 0.0019 0.0011 0.001 0.0007 0.0013 0.0003 0.001 0.0002 0.0001 0.0001 0.0002 0.0027
Bud Neck 0.0032 0.0009 0.0024 0.0023 0.0016 0.0038 0.0029 0.0006 0.0006 0.0048 0.001 0.0033 0.0084 0.0013 0.0007 0.0006 0.0025 0.0097
Bud Periphery 0.0017 0.0019 0.0007 0.0014 0.0007 0.0002 0.0086 0.001 0.0014 0.0006 0.0015 0.0004 0.0011 0.0001 0.0001 0.0001 0.0002 0.0009
Bud Site 0.002 0.0011 0.0056 0.0019 0.0026 0.0003 0.0028 0.0035 0.007 0.0043 0.0037 0.0006 0.0055 0.0027 0.0004 0.0001 0.0005 0.0004
Cell Periphery 0.0001 0 0.0003 0.0002 0.0003 0 0.0006 0.0002 0.0001 0.0002 0.0001 0.0001 0.0002 0.0001 0.0001 0 0 0.0001
Cytoplasm 0.0522 0.0894 0.0385 0.0524 0.0505 0.0446 0.1789 0.3702 0.2872 0.2344 0.1234 0.2904 0.1053 0.2052 0.1305 0.0859 0.1311 0.0983
Cytoplasmic Foci 0.0084 0.0034 0.0053 0.0042 0.0086 0.0019 0.0518 0.0133 0.0131 0.0155 0.0178 0.0103 0.0111 0.0041 0.0133 0.0039 0.0081 0.0085
Eisosomes 0.0003 0.0001 0.0003 0.0003 0.0008 0.0001 0.0002 0.0001 0.0001 0.0002 0.0002 0.0001 0.0002 0 0.0001 0.0001 0.0002 0.0001
Endoplasmic Reticulum 0.0029 0.003 0.0019 0.0024 0.0067 0.002 0.0056 0.0075 0.0072 0.0058 0.0145 0.0047 0.0055 0.0053 0.005 0.0074 0.0027 0.0026
Endosome 0.0144 0.0037 0.0079 0.0156 0.0259 0.0056 0.0352 0.0117 0.008 0.0178 0.0166 0.009 0.0134 0.0048 0.0084 0.0068 0.0127 0.009
Golgi 0.0041 0.0002 0.003 0.0063 0.0064 0.0012 0.0246 0.0032 0.002 0.0284 0.0178 0.0006 0.0028 0.0001 0.001 0.0001 0.003 0.0011
Lipid Particles 0.0091 0.0004 0.0072 0.0122 0.0094 0.0037 0.0337 0.0025 0.0073 0.0092 0.0062 0.0019 0.0108 0.0003 0.0032 0.0005 0.0181 0.0053
Mitochondria 0.0132 0.0011 0.0046 0.0061 0.0067 0.0052 0.0564 0.0026 0.0021 0.0122 0.0246 0.0208 0.0017 0.0005 0.0009 0.0008 0.0017 0.0013
None 0.099 0.1488 0.172 0.1517 0.0995 0.0975 0.2723 0.4269 0.3758 0.0978 0.3996 0.1487 0.116 0.1056 0.2133 0.2591 0.1471 0.1288
Nuclear Periphery 0.0335 0.0274 0.031 0.0145 0.055 0.0266 0.0304 0.0144 0.0176 0.0225 0.0459 0.0185 0.0244 0.0254 0.0296 0.0401 0.0527 0.0239
Nucleolus 0.013 0.0117 0.0172 0.0078 0.032 0.0205 0.0032 0.0031 0.0029 0.0055 0.0012 0.0042 0.008 0.0105 0.0077 0.0026 0.0069 0.0142
Nucleus 0.6397 0.6524 0.5883 0.6673 0.5703 0.7206 0.2171 0.1076 0.1993 0.4229 0.2289 0.4369 0.5987 0.5775 0.5276 0.489 0.4582 0.6187
Peroxisomes 0.0119 0.0022 0.0098 0.0052 0.0015 0.0009 0.0187 0.0009 0.0084 0.0105 0.0237 0.0011 0.0084 0.0001 0.0023 0.0003 0.0024 0.0043
Punctate Nuclear 0.0724 0.0475 0.0767 0.0297 0.0679 0.061 0.0177 0.019 0.0309 0.0084 0.0175 0.0414 0.0509 0.0519 0.0509 0.0973 0.1467 0.0658
Vacuole 0.0026 0.0022 0.0027 0.0016 0.004 0.0012 0.0109 0.0051 0.0068 0.006 0.0046 0.0043 0.0032 0.0037 0.0018 0.0036 0.0015 0.002
Vacuole Periphery 0.0012 0.0005 0.001 0.0009 0.0027 0.0006 0.0051 0.0011 0.0009 0.0008 0.0012 0.0009 0.001 0.0005 0.0006 0.0012 0.0011 0.0006

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 1.3522 8.8645 4.1859 15.55 7.0128 3.6039 4.4814 6.0493 10.0472 6.3743
Translational Efficiency

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
318 255 42 1311 1700 1772 907 1320 2018 2027 949 2631

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 668.10 769.16 1023.64 904.91 649.75 704.90 891.10 921.24 652.64 712.98 896.97 913.10
Standard Deviation 74.90 83.58 129.09 141.61 81.21 80.51 145.01 127.93 80.53 83.66 146.89 135.17
Intensity Change Log 2 0.203220 0.615572 0.437710 0.117534 0.455703 0.503692 0.161609 0.538962 0.470619

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000056 0.000707 0.002552 0.001753 0.000064 0.000190 0.000371 0.001540 0.000063 0.000255 0.000468 0.001646
Bud Neck 0.012839 0.014180 0.005785 0.020335 0.004092 0.011203 0.007587 0.021770 0.005471 0.011578 0.007507 0.021055
Bud Site 0.001577 0.010102 0.003070 0.017445 0.001449 0.003961 0.004250 0.029763 0.001469 0.004734 0.004198 0.023625
Cell Periphery 0.000135 0.000184 0.000348 0.000217 0.000116 0.000166 0.000811 0.000228 0.000119 0.000169 0.000790 0.000222
Cytoplasm 0.355970* 0.287837* 0.041720 0.142083* 0.420078* 0.312944* 0.095729 0.183663* 0.409976* 0.309786* 0.093339 0.162944*
Cytoplasmic Foci 0.268517* 0.226339* 0.003394 0.006884 0.077525 0.115068 0.012448 0.013447 0.107622 0.129066 0.012048 0.010177
Eisosomes 0.000177 0.000148 0.000110 0.000049 0.000043 0.000069 0.000433 0.000045 0.000064 0.000079 0.000419 0.000047
Endoplasmic Reticulum 0.000580 0.002567 0.021338 0.016939 0.001032 0.001541 0.003046 0.007817 0.000960 0.001670 0.003855 0.012362
Endosome 0.007807 0.021272 0.004549 0.008414 0.002088 0.005467 0.001153 0.012617 0.002989 0.007456 0.001303 0.010522
Golgi 0.001391 0.007127 0.000283 0.006648 0.000373 0.002412 0.000430 0.007250 0.000533 0.003005 0.000423 0.006950
Lipid Particles 0.014010 0.014015 0.002835 0.002123 0.004269 0.010656 0.001799 0.001318 0.005804 0.011079 0.001845 0.001719
Mitochondria 0.006040 0.008388 0.001783 0.010722 0.002659 0.012629 0.000992 0.007013 0.003192 0.012095 0.001027 0.008861
Mitotic Spindle 0.001288 0.002051 0.002909 0.052577 0.001184 0.008003 0.002575 0.053031 0.001201 0.007254 0.002590 0.052805
None 0.003125 0.004277 0.003314 0.007971 0.018072 0.007204 0.037446 0.007478 0.015717 0.006836 0.035935 0.007724
Nuclear Periphery 0.000642 0.001590 0.002050 0.006144 0.000957 0.002349 0.000850 0.004446 0.000907 0.002254 0.000903 0.005292
Nuclear Periphery Foci 0.000315 0.001969 0.006279 0.005231 0.000927 0.000890 0.000647 0.002470 0.000830 0.001026 0.000897 0.003846
Nucleolus 0.001616 0.004118 0.018675 0.007386 0.003425 0.005389 0.010062 0.007350 0.003140 0.005229 0.010443 0.007368
Nucleus 0.283038* 0.322102* 0.845066* 0.631371* 0.452333* 0.474010* 0.811914* 0.562818* 0.425655* 0.454900* 0.813381* 0.596977*
Peroxisomes 0.007875 0.026249 0.001242 0.000671 0.001233 0.007784 0.000500 0.001364 0.002280 0.010107 0.000533 0.001018
Vacuole 0.032678 0.043239 0.032340 0.052384 0.007652 0.017191 0.006537 0.064045 0.011595 0.020468 0.007679 0.058235
Vacuole Periphery 0.000322 0.001539 0.000357 0.002655 0.000432 0.000872 0.000418 0.010527 0.000414 0.000956 0.000416 0.006605

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.67 -6.99 -10.31 -3.67 3.65 -5.41 -11.18 -8.73 -7.65 -4.39 -6.34 -12.33 -13.46 -10.97 -5.91
Bud Neck -0.42 1.22 -2.99 -3.34 -8.08 -6.19 -4.14 -9.73 -4.34 -5.64 -5.28 -2.84 -13.18 -6.81 -8.60
Bud Site -3.56 -3.35 -8.11 -0.76 -5.32 -3.07 -2.38 -6.81 -5.21 -4.70 -4.24 -2.49 -10.23 -6.44 -5.87
Cell Periphery -2.36 -3.33 -3.05 0.33 2.74 -1.01 -6.56 -2.75 0.23 6.08 -1.16 -6.66 -5.47 -0.02 6.06
Cytoplasm 3.33 16.46 16.87 11.12 -5.04 12.07 39.86 31.49 19.22 -8.32 12.30 41.82 39.72 25.56 -7.64
Cytoplasmic Foci 2.23 20.61 20.72 16.40 -0.38 -7.44 20.49 22.13 26.12 1.99 -4.13 25.81 28.06 30.62 3.25
Eisosomes 1.39 1.32 7.75 6.91 3.42 -7.77 -3.87 -7.99 0.98 3.57 -3.90 -3.64 -0.63 3.97 3.62
Endoplasmic Reticulum -4.32 -6.52 -22.45 -17.34 1.80 -2.49 -11.01 -22.37 -19.71 -11.88 -3.77 -12.14 -30.15 -26.76 -15.55
Endosome -3.32 0.90 2.19 4.49 0.67 -5.90 2.74 -4.16 0.18 -5.26 -6.11 4.15 -3.31 3.17 -6.50
Golgi -1.59 1.36 -0.11 1.60 -2.26 -4.27 0.02 -2.50 0.51 -2.41 -4.05 0.95 -2.73 1.29 -3.18
Lipid Particles -0.27 3.84 4.55 5.68 1.61 -7.98 4.95 6.95 12.28 -0.14 -6.44 6.35 7.62 13.36 -0.19
Mitochondria -0.56 1.32 0.23 1.05 -2.71 -6.17 3.71 -1.40 5.31 -4.00 -5.85 3.41 -1.46 4.90 -4.88
Mitotic Spindle -1.10 -1.22 -10.28 -9.96 -8.48 -4.49 -2.17 -9.34 -6.89 -8.36 -4.59 -2.30 -13.92 -10.86 -12.46
None -1.05 -0.42 -3.23 -2.19 -2.36 6.51 -3.41 7.85 1.27 6.91 6.18 -3.93 7.56 1.07 6.91
Nuclear Periphery -3.00 -3.70 -16.79 -12.15 -8.72 -6.83 -2.35 -15.07 -10.57 -13.81 -7.42 -3.25 -21.85 -15.81 -19.52
Nuclear Periphery Foci -4.56 -2.58 -8.43 -4.54 0.64 -0.36 -1.50 -5.06 -5.03 -3.12 -1.58 -2.23 -8.62 -8.06 -4.38
Nucleolus -2.92 -2.66 -6.55 -3.20 1.96 -3.66 -5.57 -3.57 -1.23 2.54 -4.29 -6.28 -6.04 -2.53 2.85
Nucleus -1.63 -14.93 -21.05 -14.82 5.38 -1.94 -33.78 -11.99 -9.96 18.82 -2.87 -38.15 -20.10 -16.21 20.80
Peroxisomes -3.51 4.64 5.74 5.24 2.70 -8.85 5.86 1.83 9.09 -0.93 -8.43 7.31 6.40 10.53 -0.44
Vacuole -1.43 -0.71 -11.21 -9.08 -3.63 -8.40 -1.49 -21.22 -19.33 -20.25 -6.48 0.22 -27.04 -24.40 -25.60
Vacuole Periphery -3.18 -1.81 -2.88 1.23 -2.18 -4.44 0.27 -3.18 -2.62 -3.14 -5.48 0.19 -3.74 -2.41 -3.40
Standard name
Human Ortholog
Description Transcription factor, allelic differences between S288C and Sigma1278b; mediates fermentation stress tolerance by modulating cell wall integrity; overexpression suppresses heat shock sensitivity of wild-type RAS2 overexpression and also suppresses cell lysis defect of mpk1 mutation; allele from S288c can confer fMAPK pathway independent transcription of FLO11; S288C and Sigma1278b alleles differ in number of tandem repeats within ORF
Localization
Cell Percentages cytoplasm (19%), nucleus (18%), mixed (52%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Rpi1

Rpi1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rpi1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available