Standard name
Human Ortholog
Description Plasma membrane transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; exports copper; has broad substrate specificity and can transport many mono- and divalent cations; transports a variety of drugs and is required for resistance to quinidine, barban, cisplatin, and bleomycin; contributes to potassium homeostasis; expression is regulated by copper
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.21 0.33 0.41 0.36 0.37 0.34 0.25 0.16 – 0.15 0.16 0.09 0.15 0.15 0.09 0.12 0.08 0.1 0 0 0 0 0 0
Bud 0 0 0.07 0.07 0.07 0.11 0.1 0.14 – 0.15 0.17 0.11 0 0 0 0.05 0.05 0.08 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.93 0.84 0.85 0.83 0.78 0.62 0.51 0.29 – 0.33 0.32 0.23 0.91 0.91 0.92 0.86 0.81 0.74 0.94 0.86 0.89 0.93 0.89 0.9
Cytoplasm 0.05 0.15 0.1 0.17 0.16 0.34 0.48 0.54 – 0.64 0.68 0.73 0.05 0.05 0 0.18 0.22 0.18 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0.06 0.06 0.07 – 0.07 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.06 0 0.08 0 0 0 0.05 – 0.05 0.07 0.05 0 0 0 0.05 0.07 0.12 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0.05 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 45 32 120 92 124 106 89 82 – 75 67 43 138 137 74 19 10 9 5 2 7 3 16 15
Bud 4 4 19 17 23 33 35 72 – 76 72 53 14 14 8 8 6 7 0 0 1 1 2 6
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 1 1 3 9 7
Bud Site 0 0 0 0 1 2 0 1 – 4 1 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 198 81 248 213 257 195 184 147 – 163 137 110 814 814 743 138 105 65 225 100 301 374 530 560
Cytoplasm 10 15 30 43 53 106 175 275 – 320 292 344 47 47 30 29 28 16 0 4 3 1 5 6
Endoplasmic Reticulum 2 0 3 6 11 20 20 37 – 33 16 17 18 20 22 2 2 5 1 1 11 4 10 10
Endosome 0 0 0 0 0 1 1 0 – 3 0 1 0 0 0 0 0 0 1 0 0 0 1 0
Golgi 1 0 0 0 0 0 0 0 – 0 0 0 1 2 1 0 0 0 1 0 0 0 0 1
Mitochondria 3 6 12 20 1 5 9 27 – 24 29 25 3 3 2 8 9 11 2 0 5 5 9 5
Nucleus 2 0 0 1 0 0 3 4 – 5 2 2 2 2 5 2 0 1 0 0 0 0 0 0
Nuclear Periphery 0 0 0 1 0 0 0 24 – 1 1 0 0 0 0 0 0 0 0 1 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 1 0 0 0 0 1 2 0 – 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 1 0 0 2 2 4 3 3 – 1 2 5 5 4 4 0 0 0 0 0 0 0 0 1
Vac/Vac Membrane 0 0 0 0 0 0 1 17 – 6 5 20 1 1 1 0 0 0 0 2 0 5 5 5
Unique Cell Count 214 97 292 257 331 312 362 506 500 431 470 896 891 804 160 129 88 240 117 337 404 595 625
Labelled Cell Count 267 138 432 395 472 473 522 689 711 624 622 1043 1044 890 206 160 114 240 117 337 404 595 625


Cell Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 12.8 14.2 11.4 11.1 11.9 9.5 8.6 7.6 – 6.4 6.5 6.0 13.8 13.8 15.9 16.0 19.2 23.0 13.1 12.6 13.1
Std Deviation (1e-4) 2.4 3.1 2.5 2.2 2.5 2.3 2.4 2.1 – 1.8 1.8 1.5 1.9 1.9 2.5 3.6 5.4 5.7 2.4 2.5 2.6
Intensity Change (Log2) – – – -0.04 0.06 -0.27 -0.42 -0.58 – -0.84 -0.81 -0.93 0.27 0.27 0.48 0.49 0.75 1.01 0.2 0.15 0.19


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -1.3 -0.9 -1.8 -4.5 -7.8 – -8.2 -7.7 -10.5 -9.2 -9.2 -12.2 -6.4 -6.8 -5.4
Bud 0.1 0.2 1.8 1.5 3.3 – 3.6 4.1 2.2 -4.5 -4.4 -5.2 -0.6 -0.7 0.5
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cell Periphery -0.7 -2.3 -6.2 -9.2 -15.2 – -14.2 -14.1 -16.5 2.9 3.1 3.7 0.4 -0.9 -2.4
Cytoplasm 2.2 2.1 7.0 10.4 12.3 – 14.7 15.3 16.9 -3.0 -3.0 -4.2 2.4 3.1 2.0
Endoplasmic Reticulum 0 1.9 3.5 3.1 3.9 – 3.6 2.2 2.2 1.1 1.3 1.7 0 0 0
Endosome 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Mitochondria 1.8 -3.3 -1.9 -1.2 0.8 – 0.5 1.5 0.8 0 0 0 0.4 1.2 2.9
Nucleus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 3.8 – 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 3.2 – 0 0 3.6 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 7.6583 11.5 10.2461 9.8148 8.8692 9.754 5.5894 10.8194 7.6014 7.3124 6.6703 8.0125 8.6482 11.5492 10.2527 10.4903 8.5877 11.1204
Actin 0.0719 0.0005 0.0251 0.0333 0.0189 0.0104 0.0271 0.0037 0.0113 0.0002 0.0015 0.0006 0.0004 0.0009 0.0004 0.0005 0.0286 0
Bud 0.0015 0.0004 0.0065 0.0005 0.0114 0.0003 0.0012 0.0007 0.0007 0.0003 0.001 0.0008 0.0001 0.0002 0.0002 0.001 0.0003 0.0001
Bud Neck 0.0045 0.0023 0.0029 0.0032 0.0044 0.0029 0.0048 0.0006 0.0026 0.0012 0.0029 0.0011 0.0009 0.0007 0.0016 0.0011 0.0023 0.0007
Bud Periphery 0.0088 0.0029 0.0052 0.007 0.0479 0.0063 0.0082 0.0046 0.0059 0.0082 0.0186 0.015 0.002 0.0018 0.0027 0.0116 0.0031 0.0047
Bud Site 0.0211 0.0312 0.0178 0.0017 0.0051 0.0005 0.0252 0.0282 0.0076 0.0011 0.0025 0.0006 0.0031 0.0204 0.0116 0.0019 0.0013 0.0002
Cell Periphery 0.6455 0.7707 0.7987 0.7423 0.7215 0.8741 0.6649 0.8802 0.8326 0.9041 0.848 0.9174 0.8804 0.8912 0.8991 0.9122 0.8248 0.9348
Cytoplasm 0.0701 0.1517 0.0924 0.0691 0.0682 0.0375 0.0803 0.0521 0.0475 0.0476 0.0485 0.0269 0.045 0.0472 0.0347 0.0121 0.0207 0.0194
Cytoplasmic Foci 0.012 0.0001 0.0012 0.0041 0.0028 0.0007 0.0374 0.0003 0.0078 0 0.0002 0.0001 0.0027 0.0001 0.0002 0 0.0026 0
Eisosomes 0.0046 0.0005 0.0009 0.0002 0.0003 0.001 0.0031 0.0003 0.0009 0.0008 0.001 0.0004 0.0017 0.0004 0.0008 0.0008 0.0029 0.0001
Endoplasmic Reticulum 0.0335 0.0179 0.0099 0.0373 0.006 0.0206 0.02 0.0115 0.0186 0.0148 0.0337 0.0074 0.0234 0.0131 0.0193 0.0281 0.0252 0.0121
Endosome 0.0136 0.0001 0.0014 0.0017 0.0137 0.0061 0.0212 0.0003 0.0076 0 0.0009 0.0001 0.0021 0 0 0 0.0036 0
Golgi 0.0084 0.0001 0.0014 0.0029 0.0088 0.003 0.0074 0.0007 0.0034 0.0001 0.0005 0.0003 0.0004 0 0.0001 0 0.0035 0
Lipid Particles 0.0144 0.0039 0.003 0.0136 0.0104 0.0024 0.024 0.0006 0.0065 0.0011 0.0038 0.001 0.0024 0.0009 0.0007 0.001 0.0121 0.0009
Mitochondria 0.0148 0 0.0022 0.0009 0.0137 0.0012 0.0017 0.0001 0.0134 0 0.0016 0.0003 0.0001 0 0 0 0.0013 0
None 0.0107 0.0001 0.0005 0.0005 0.0009 0.0003 0.0022 0.0001 0.0036 0.0001 0.0004 0.0001 0.0006 0.0001 0.0051 0.0001 0.0137 0.0001
Nuclear Periphery 0.009 0.0013 0.001 0.0013 0.0038 0.0073 0.0197 0.0007 0.0013 0.0008 0.0013 0.0004 0.003 0.0018 0.0015 0.0014 0.006 0.0008
Nucleolus 0.0062 0.0001 0.0014 0.0001 0.0005 0.0001 0.001 0 0.0002 0 0.0003 0.0001 0.0004 0 0.0001 0 0.0063 0
Nucleus 0.0079 0.0003 0.0038 0.0003 0.0016 0.0008 0.004 0.0002 0.0004 0.0001 0.0007 0.0001 0.0008 0.0001 0.0002 0.0001 0.0084 0.0001
Peroxisomes 0.0074 0 0.0063 0.034 0.0053 0.0003 0.0059 0 0.0008 0 0 0 0.0001 0 0 0 0.001 0
Punctate Nuclear 0.0039 0 0.0003 0.0003 0.0019 0.0008 0.003 0 0.0003 0 0 0 0.0052 0 0.0001 0 0.0238 0
Vacuole 0.0282 0.0159 0.018 0.0454 0.0515 0.0228 0.0357 0.0149 0.0267 0.0195 0.0316 0.027 0.0251 0.0212 0.0216 0.0279 0.0081 0.0258
Vacuole Periphery 0.002 0 0.0002 0.0002 0.0015 0.0004 0.0019 0.0001 0.0005 0.0001 0.0009 0.0002 0.0001 0.0001 0.0001 0.0001 0.0005 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 18.2885 21.1929 32.8 32.8003 30.2984 34.5258 32.1421 31.6109 38.2858 33.0224
Translational Efficiency 0.4994 0.6685 0.4724 0.581 0.45 0.3697 0.5526 0.5838 0.5269 0.5055

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Plasma membrane transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; exports copper; has broad substrate specificity and can transport many mono- and divalent cations; transports a variety of drugs and is required for resistance to quinidine, barban, cisplatin, and bleomycin; contributes to potassium homeostasis; expression is regulated by copper
Localization
Cell Percentages cell periphery (75%)
Cell Cycle Regulation No
Subcompartmental Group cell periphery-1

Qdr2

Qdr2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Qdr2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available