Standard name | |
---|---|
Human Ortholog | |
Description | Protein of the SUN family (Sim1p, Uth1p, Nca3p, Sun4p); may participate in DNA replication; promoter contains SCB regulation box at -300 bp indicating that expression may be cell cycle-regulated; SIM1 has a paralog, SUN4, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 2 | 2 |
Bud | 2 | 0 | 1 | 1 | 1 | 2 | 0 | 3 | 1 | 0 | 1 | 2 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 3 | 0 | 1 | 8 | 7 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 2 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 3 | 2 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 87 | 139 | 93 | 133 | 124 | 222 | 210 | 329 | 211 | 230 | 213 | 229 | 190 | 305 | 340 | 564 | 516 | 356 | 30 | 48 | 17 | 50 | 65 | 89 |
Endoplasmic Reticulum | 0 | 1 | 0 | 4 | 3 | 3 | 1 | 4 | 4 | 2 | 3 | 1 | 1 | 2 | 0 | 47 | 38 | 18 | 5 | 0 | 3 | 3 | 3 | 1 |
Endosome | 5 | 3 | 1 | 2 | 1 | 5 | 4 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 16 | 5 | 13 | 10 | 6 | 2 | 1 | 1 | 3 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 3 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 1 |
Mitochondria | 29 | 3 | 4 | 5 | 1 | 9 | 18 | 22 | 25 | 38 | 25 | 41 | 0 | 0 | 0 | 18 | 4 | 3 | 7 | 2 | 7 | 8 | 5 | 5 |
Nucleus | 2 | 0 | 0 | 1 | 2 | 2 | 8 | 16 | 1 | 5 | 1 | 5 | 0 | 0 | 0 | 10 | 17 | 14 | 0 | 2 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 2 | 1 | 0 | 1 | 0 | 3 | 2 | 1 | 0 | 2 | 3 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 2 |
Vac/Vac Membrane | 37 | 47 | 17 | 13 | 73 | 71 | 86 | 84 | 44 | 55 | 18 | 36 | 2 | 0 | 2 | 178 | 233 | 150 | 106 | 125 | 75 | 76 | 119 | 106 |
Unique Cell Count | 142 | 177 | 109 | 151 | 174 | 275 | 268 | 406 | 252 | 275 | 239 | 273 | 192 | 306 | 342 | 675 | 646 | 465 | 169 | 198 | 115 | 148 | 216 | 231 |
Labelled Cell Count | 164 | 194 | 116 | 164 | 206 | 317 | 329 | 467 | 287 | 332 | 264 | 315 | 195 | 308 | 346 | 839 | 818 | 556 | 169 | 198 | 115 | 148 | 216 | 231 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.7 | 5.1 | 4.4 | 4.7 | 5.2 | 5.2 | 4.6 | 4.9 | 5.0 | 4.3 | 4.7 | 4.3 | 4.7 | 4.5 | 4.4 | 5.3 | 6.3 | 5.8 | 4.4 | 4.7 | 5.3 |
Std Deviation (1e-4) | 0.8 | 1.7 | 1.5 | 1.1 | 1.3 | 1.5 | 1.3 | 1.6 | 1.1 | 1.1 | 1.2 | 1.4 | 1.3 | 1.1 | 1.2 | 1.4 | 1.6 | 1.1 | 1.4 | 1.8 | 1.7 |
Intensity Change (Log2) | – | – | – | 0.11 | 0.26 | 0.25 | 0.08 | 0.17 | 0.18 | -0.02 | 0.1 | -0.02 | 0.1 | 0.02 | 0.02 | 0.28 | 0.52 | 0.39 | -0.01 | 0.09 | 0.27 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.7 | -2.7 | -1.1 | -1.5 | -1.0 | -0.4 | -0.4 | 1.0 | -0.3 | 4.8 | 6.5 | 6.5 | -0.5 | -1.3 | -2.0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.8 | 2.6 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0.7 | 2.0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -1.7 | 4.6 | 2.1 | 3.3 | 1.2 | 0.4 | 1.0 | -2.3 | -0.6 | -5.0 | 0 | 0 | 2.4 | 4.2 | 3.4 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.0123 | 0.7009 | 0.254 | 0.4964 | -0.1484 | 0.4423 | 3.0353 | 2.919 | 3.1467 | 3.6611 | 2.5829 | 3.0644 | 3.6603 | 3.3778 | 3.612 | 3.9852 | 3.6907 | 3.9947 |
Actin | 0.0215 | 0.0001 | 0.0001 | 0 | 0.0117 | 0.0058 | 0.0325 | 0.0004 | 0.0282 | 0.0689 | 0.0003 | 0.0021 | 0.0293 | 0.0001 | 0.0214 | 0.0006 | 0.0005 | 0.0001 |
Bud | 0.0101 | 0.0007 | 0.0051 | 0.0002 | 0.0011 | 0.0003 | 0.0015 | 0.0036 | 0.0064 | 0.0013 | 0.0051 | 0.0011 | 0.0012 | 0.0033 | 0.0006 | 0.0208 | 0.0018 | 0.0005 |
Bud Neck | 0.0015 | 0.0007 | 0.0009 | 0.0011 | 0.0018 | 0.0028 | 0.0029 | 0.0028 | 0.006 | 0.0015 | 0.0028 | 0.0053 | 0.001 | 0.0019 | 0.0005 | 0.0014 | 0.0015 | 0.0015 |
Bud Periphery | 0.0054 | 0.001 | 0.0022 | 0.0002 | 0.0015 | 0.0006 | 0.0041 | 0.0056 | 0.0149 | 0.0009 | 0.0114 | 0.0029 | 0.002 | 0.0017 | 0.0012 | 0.0116 | 0.0073 | 0.0008 |
Bud Site | 0.0023 | 0.0049 | 0.0077 | 0.0005 | 0.0048 | 0.0006 | 0.0058 | 0.0154 | 0.0093 | 0.0111 | 0.0061 | 0.0011 | 0.0018 | 0.0078 | 0.0017 | 0.01 | 0.0039 | 0.0009 |
Cell Periphery | 0.0014 | 0.0008 | 0.0006 | 0.0003 | 0.0004 | 0.0008 | 0.0012 | 0.0018 | 0.001 | 0.0004 | 0.0012 | 0.0009 | 0.001 | 0.0012 | 0.0006 | 0.0013 | 0.002 | 0.0008 |
Cytoplasm | 0.1156 | 0.2065 | 0.1365 | 0.0386 | 0.2026 | 0.2455 | 0.161 | 0.2224 | 0.1356 | 0.1126 | 0.1485 | 0.2265 | 0.2555 | 0.257 | 0.1614 | 0.1335 | 0.1647 | 0.2056 |
Cytoplasmic Foci | 0.0696 | 0.044 | 0.0615 | 0.0362 | 0.0547 | 0.0553 | 0.0568 | 0.0624 | 0.0599 | 0.0856 | 0.0722 | 0.0531 | 0.0499 | 0.0208 | 0.0569 | 0.0332 | 0.0403 | 0.0406 |
Eisosomes | 0.0003 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0004 | 0 | 0.0004 | 0.0002 | 0 | 0.0001 | 0.0003 | 0 | 0.0002 | 0.0001 | 0.0002 | 0.0002 |
Endoplasmic Reticulum | 0.0102 | 0.0094 | 0.0037 | 0.002 | 0.0027 | 0.0141 | 0.0113 | 0.009 | 0.0063 | 0.0035 | 0.0033 | 0.0106 | 0.0222 | 0.0111 | 0.0075 | 0.0034 | 0.0075 | 0.013 |
Endosome | 0.1778 | 0.1534 | 0.2545 | 0.5168 | 0.2411 | 0.1668 | 0.1558 | 0.1539 | 0.17 | 0.1999 | 0.2274 | 0.1885 | 0.1141 | 0.0866 | 0.1869 | 0.2331 | 0.1556 | 0.1758 |
Golgi | 0.0049 | 0.0023 | 0.004 | 0.003 | 0.0107 | 0.0029 | 0.0173 | 0.0027 | 0.0086 | 0.0334 | 0.0027 | 0.0035 | 0.0059 | 0.0012 | 0.0048 | 0.0031 | 0.0022 | 0.002 |
Lipid Particles | 0.0169 | 0.0091 | 0.0104 | 0.0026 | 0.0026 | 0.0097 | 0.0267 | 0.0075 | 0.0074 | 0.0081 | 0.0059 | 0.0052 | 0.0075 | 0.0026 | 0.0113 | 0.0136 | 0.0072 | 0.0064 |
Mitochondria | 0.0064 | 0.0027 | 0.0143 | 0.0008 | 0.0197 | 0.0019 | 0.0154 | 0.0046 | 0.019 | 0.0043 | 0.0039 | 0.0106 | 0.0034 | 0.0064 | 0.0057 | 0.0262 | 0.0478 | 0.0025 |
None | 0.3729 | 0.3383 | 0.2786 | 0.0215 | 0.188 | 0.2429 | 0.3032 | 0.2163 | 0.3196 | 0.2565 | 0.1682 | 0.1749 | 0.3276 | 0.2378 | 0.3066 | 0.1113 | 0.145 | 0.2269 |
Nuclear Periphery | 0.0138 | 0.008 | 0.0093 | 0.0025 | 0.0023 | 0.0064 | 0.0151 | 0.0047 | 0.0051 | 0.0027 | 0.0032 | 0.0078 | 0.0136 | 0.007 | 0.0075 | 0.0034 | 0.0055 | 0.0105 |
Nucleolus | 0.0018 | 0.0022 | 0.0048 | 0.001 | 0.0082 | 0.0016 | 0.0029 | 0.0034 | 0.0026 | 0.002 | 0.0049 | 0.0017 | 0.0013 | 0.005 | 0.0019 | 0.0067 | 0.0021 | 0.0013 |
Nucleus | 0.0063 | 0.0081 | 0.0138 | 0.0022 | 0.0504 | 0.0081 | 0.008 | 0.0103 | 0.0081 | 0.0043 | 0.0077 | 0.0108 | 0.0069 | 0.0249 | 0.0076 | 0.0081 | 0.0082 | 0.0064 |
Peroxisomes | 0.0063 | 0.0005 | 0.0029 | 0.0004 | 0.0032 | 0.0008 | 0.0131 | 0.0069 | 0.0128 | 0.0122 | 0.0037 | 0.0022 | 0.0028 | 0.0004 | 0.0041 | 0.0037 | 0.0005 | 0.0004 |
Punctate Nuclear | 0.0103 | 0.0027 | 0.004 | 0.0003 | 0.0009 | 0.0022 | 0.0126 | 0.0022 | 0.0077 | 0.0021 | 0.0016 | 0.0023 | 0.0117 | 0.0022 | 0.0046 | 0.0008 | 0.0008 | 0.0012 |
Vacuole | 0.1318 | 0.1903 | 0.1686 | 0.3397 | 0.1815 | 0.2137 | 0.1417 | 0.2491 | 0.1593 | 0.1799 | 0.2975 | 0.263 | 0.1322 | 0.299 | 0.1865 | 0.3481 | 0.3724 | 0.2775 |
Vacuole Periphery | 0.0127 | 0.0142 | 0.0164 | 0.0302 | 0.01 | 0.0171 | 0.0109 | 0.015 | 0.0118 | 0.0086 | 0.0223 | 0.0258 | 0.0088 | 0.0221 | 0.0207 | 0.0262 | 0.023 | 0.0255 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 421.0055 | 559.0154 | 345.7632 | 196.0802 | 333.4096 | 305.1756 | 543.092 | 415.8237 | 301.5221 | 291.9596 |
Translational Efficiency | 1.4321 | 1.3836 | 1.2426 | 1.4659 | 1.3053 | 1.4999 | 1.5123 | 1.483 | 1.2162 | 1.4121 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1012 | 1045 | 230 | 1437 | 1585 | 1588 | 186 | 2838 | 2597 | 2633 | 416 | 4275 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 631.66 | 688.83 | 905.14 | 833.46 | 640.53 | 670.84 | 927.73 | 899.75 | 637.07 | 677.98 | 915.24 | 877.47 |
Standard Deviation | 72.22 | 96.57 | 90.77 | 126.46 | 76.26 | 124.74 | 96.15 | 120.52 | 74.83 | 114.73 | 93.89 | 126.49 |
Intensity Change Log 2 | — | 0.125000 | 0.518993 | 0.399965 | — | 0.066703 | 0.534439 | 0.490258 | — | 0.095942 | 0.526790 | 0.446132 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000018 | 0.000289 | 0.000617 | 0.001917 | 0.000041 | 0.000883 | 0.000265 | 0.004046 | 0.000032 | 0.000647 | 0.000460 | 0.003330 |
Bud Neck | 0.006620 | 0.031731 | 0.003774 | 0.011094 | 0.009119 | 0.037308 | 0.003032 | 0.027959 | 0.008145 | 0.035094 | 0.003442 | 0.022290 |
Bud Site | 0.002821 | 0.021853 | 0.005683 | 0.039045 | 0.001941 | 0.026059 | 0.004535 | 0.050122 | 0.002284 | 0.024390 | 0.005170 | 0.046399 |
Cell Periphery | 0.000201 | 0.000106 | 0.000367 | 0.000294 | 0.000120 | 0.000225 | 0.000178 | 0.000575 | 0.000152 | 0.000178 | 0.000282 | 0.000481 |
Cytoplasm | 0.527540* | 0.255827* | 0.220911* | 0.464409* | 0.552221* | 0.197830* | 0.398176* | 0.442745* | 0.542603* | 0.220848* | 0.300169* | 0.450027* |
Cytoplasmic Foci | 0.104211 | 0.157988 | 0.030625 | 0.060297 | 0.155899 | 0.148898 | 0.030227 | 0.071030 | 0.135757 | 0.152506 | 0.030447 | 0.067422 |
Eisosomes | 0.000114 | 0.000083 | 0.000137 | 0.000087 | 0.000089 | 0.000120 | 0.000083 | 0.000320 | 0.000098 | 0.000106 | 0.000113 | 0.000241 |
Endoplasmic Reticulum | 0.000793 | 0.001769 | 0.011860 | 0.005447 | 0.001240 | 0.001432 | 0.009735 | 0.013750 | 0.001066 | 0.001566 | 0.010910 | 0.010959 |
Endosome | 0.005373 | 0.111607 | 0.018289 | 0.016144 | 0.006841 | 0.128769 | 0.009793 | 0.025818 | 0.006269 | 0.121958 | 0.014490 | 0.022566 |
Golgi | 0.000194 | 0.003680 | 0.000160 | 0.004148 | 0.000767 | 0.008045 | 0.000097 | 0.004454 | 0.000544 | 0.006313 | 0.000132 | 0.004351 |
Lipid Particles | 0.003637 | 0.006259 | 0.006424 | 0.006452 | 0.004977 | 0.011624 | 0.004694 | 0.023635 | 0.004455 | 0.009495 | 0.005651 | 0.017859 |
Mitochondria | 0.000563 | 0.006202 | 0.000577 | 0.001881 | 0.001637 | 0.012943 | 0.000182 | 0.003694 | 0.001218 | 0.010268 | 0.000400 | 0.003084 |
Mitotic Spindle | 0.000064 | 0.006382 | 0.001580 | 0.055503 | 0.000115 | 0.006164 | 0.003999 | 0.021115 | 0.000095 | 0.006250 | 0.002662 | 0.032674 |
None | 0.006952 | 0.002341 | 0.004285 | 0.008509 | 0.005793 | 0.003875 | 0.006912 | 0.001277 | 0.006244 | 0.003266 | 0.005460 | 0.003708 |
Nuclear Periphery | 0.000685 | 0.000492 | 0.003543 | 0.003290 | 0.000478 | 0.000541 | 0.008704 | 0.000995 | 0.000559 | 0.000521 | 0.005851 | 0.001766 |
Nuclear Periphery Foci | 0.000337 | 0.000633 | 0.006544 | 0.010156 | 0.000283 | 0.000651 | 0.014741 | 0.003079 | 0.000304 | 0.000643 | 0.010209 | 0.005457 |
Nucleolus | 0.000888 | 0.002875 | 0.001624 | 0.003542 | 0.001169 | 0.003365 | 0.000453 | 0.000619 | 0.001059 | 0.003170 | 0.001100 | 0.001601 |
Nucleus | 0.295239* | 0.039263 | 0.471258* | 0.084330 | 0.210610* | 0.028394 | 0.154804* | 0.058635 | 0.243589* | 0.032708 | 0.329767* | 0.067272 |
Peroxisomes | 0.000739 | 0.010324 | 0.001015 | 0.001369 | 0.001808 | 0.010669 | 0.000475 | 0.006918 | 0.001391 | 0.010532 | 0.000773 | 0.005053 |
Vacuole | 0.042035 | 0.331052* | 0.210326* | 0.219480* | 0.044395 | 0.362211* | 0.348726* | 0.237593* | 0.043475 | 0.349844* | 0.272207* | 0.231505* |
Vacuole Periphery | 0.000976 | 0.009241 | 0.000401 | 0.002606 | 0.000457 | 0.009995 | 0.000190 | 0.001623 | 0.000659 | 0.009696 | 0.000307 | 0.001953 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -6.43 | -10.80 | -2.42 | -1.65 | -0.19 | -3.67 | -8.88 | -8.90 | -5.32 | -7.86 | -4.44 | -12.88 | -8.96 | -5.76 | -6.52 |
Bud Neck | -11.12 | 1.27 | -2.37 | 10.02 | -4.15 | -13.39 | 5.72 | -10.00 | 6.37 | -16.70 | -17.32 | 4.90 | -10.32 | 10.16 | -16.11 |
Bud Site | -10.05 | -1.91 | -6.89 | 0.98 | -5.44 | -12.43 | -4.42 | -17.67 | -4.51 | -16.31 | -15.91 | -3.62 | -18.53 | -3.83 | -15.87 |
Cell Periphery | 7.40 | -3.28 | 2.11 | -2.61 | 4.25 | -2.63 | -2.93 | -10.99 | -4.28 | -8.82 | -0.98 | -4.76 | -9.15 | -5.24 | -3.52 |
Cytoplasm | 23.97 | 25.44 | 13.46 | -10.88 | -15.13 | 40.32* | 11.80 | 15.60 | -27.51 | -5.65 | 46.12* | 24.95 | 20.33 | -27.97 | -14.44 |
Cytoplasmic Foci | -8.31 | 18.16 | 14.43 | 21.26 | -6.66 | 1.08 | 25.13 | 20.16 | 18.39 | -10.62 | -3.65 | 31.27 | 24.02 | 27.01 | -13.64 |
Eisosomes | 4.57 | -3.07 | 6.26 | 1.60 | 6.92 | -2.69 | -1.08 | -6.06 | -4.43 | -5.57 | -0.87 | -3.82 | -5.06 | -4.36 | -3.31 |
Endoplasmic Reticulum | -1.88 | -12.34 | -7.42 | -4.18 | 9.25 | -0.39 | -4.23 | -7.95 | -8.53 | -1.83 | -1.36 | -11.19 | -9.35 | -9.21 | 2.87 |
Endosome | -21.48 | -1.91 | -3.36 | 20.72 | 0.88 | -29.99* | 0.23 | -10.40 | 26.02 | -9.58 | -36.79* | -1.70 | -10.89 | 32.87 | -3.12 |
Golgi | -8.82 | 0.86 | -2.49 | 3.54 | -2.58 | -9.29 | 5.00 | -4.26 | 5.49 | -5.74 | -11.51 | 4.94 | -5.02 | 6.21 | -6.26 |
Lipid Particles | -3.52 | -3.36 | -1.20 | 2.58 | 2.28 | -5.81 | 1.87 | -11.71 | -4.54 | -12.05 | -6.68 | -0.78 | -11.75 | -4.23 | -10.60 |
Mitochondria | -4.98 | -1.04 | -2.44 | 4.54 | -2.25 | -8.15 | 4.22 | -2.06 | 7.21 | -5.79 | -9.50 | 3.33 | -2.70 | 8.30 | -5.72 |
Mitotic Spindle | -3.63 | -3.42 | -10.80 | -8.24 | -10.08 | -5.46 | -1.98 | -10.12 | -5.57 | -2.28 | -6.43 | -2.58 | -14.74 | -9.68 | -8.20 |
None | 3.59 | 2.73 | 0.96 | -3.45 | -2.31 | 1.64 | 1.02 | 6.17 | 2.93 | 3.51 | 3.46 | 2.80 | 5.17 | 0.80 | 1.82 |
Nuclear Periphery | 1.14 | -12.49 | -10.18 | -11.05 | -0.60 | -0.99 | -2.28 | -6.81 | -5.86 | 1.74 | 0.38 | -4.81 | -11.91 | -12.20 | 2.36 |
Nuclear Periphery Foci | -2.00 | -3.13 | -7.88 | -7.58 | 0.34 | -3.29 | -3.69 | -9.00 | -7.27 | 2.42 | -3.82 | -4.74 | -11.60 | -10.46 | 2.23 |
Nucleolus | -3.64 | -1.59 | -2.20 | 1.29 | -0.58 | -4.49 | 5.87 | 4.86 | 5.73 | -2.53 | -5.77 | -0.04 | -0.16 | 5.25 | -0.06 |
Nucleus | 32.68* | -10.11 | 19.60 | -17.38 | 18.81 | 33.66 | 0.90 | 26.08 | -15.69 | 7.64 | 46.10* | -7.87 | 32.74 | -22.52 | 18.25 |
Peroxisomes | -9.88 | 0.20 | -1.35 | 9.67 | -1.60 | -11.51 | 9.00 | -7.04 | 6.12 | -10.52 | -15.16 | 6.52 | -7.34 | 10.04 | -10.22 |
Vacuole | -32.78* | -12.45 | -36.03 | -0.42 | -5.22 | -39.61* | -16.25 | -46.67 | 6.96 | 5.33 | -51.26* | -19.45 | -59.04 | 5.45 | 0.92 |
Vacuole Periphery | -8.61 | 1.34 | 0.06 | 9.62 | -2.35 | -18.19 | 9.65 | -3.34 | 15.79 | -4.91 | -18.41 | 2.17 | -1.66 | 17.96 | -5.14 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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