Standard name
Human Ortholog
Description Component of cytosolic iron-sulfur protein assembly (CIA) machinery; acts at a late step of Fe-S cluster assembly; forms the CIA targeting complex with Cia1p and Cia2p that directs Fe-S cluster incorporation and maturation of a subset of cytosolic and nuclear proteins involved in methionine biosynthesis, DNA replication and repair, transcription, and telomere maintenance; ortholog of human MMS19
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.09 0.07 0 0 0 0 0 0
Cytoplasm 0.98 0.97 1.0 0.99 0.99 0.96 0.9 0.87 0.81 0.82 0.73 0.73 0.99 0.99 0.98 0.96 0.96 0.95 0.98 0.96 0.96 0.96 0.94 0.95
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.13 0.17 0.18 0.34 0.31 0.4 0.39 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 1
Bud 0 1 1 2 3 3 2 3 4 6 2 4 0 0 2 2 1 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 5 0 3 1 5 5 15 16 9 11 8 9 0 6 7 32 26 53 0 0 0 0 0 0
Cytoplasm 180 31 87 189 417 217 492 405 218 261 131 266 70 340 376 535 279 696 181 54 96 86 211 334
Endoplasmic Reticulum 1 0 0 1 4 0 3 2 1 1 1 4 0 0 4 28 8 29 0 0 1 0 4 7
Endosome 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 2 2 2 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 1 0 0 0 0 1
Mitochondria 0 1 2 7 4 29 95 86 91 98 72 142 0 1 10 8 5 8 0 0 0 0 0 0
Nucleus 1 0 0 0 0 1 1 2 0 1 0 0 1 0 0 1 0 1 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 1 5 12 0 1 0 6 0 1 0 14 12 23 0 1 1 0 2 0
Unique Cell Count 184 32 87 191 420 226 544 468 270 318 180 363 71 342 385 555 291 729 185 57 101 90 225 354
Labelled Cell Count 187 33 93 200 433 256 613 529 324 380 214 432 71 348 399 625 334 813 185 57 101 90 225 354


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 10.0 7.4 5.9 6.2 6.1 5.4 5.0 5.0 4.5 4.5 4.4 4.2 9.5 9.5 8.3 9.3 10.3 10.7 9.1 8.8 9.5
Std Deviation (1e-4) 1.2 0.9 0.7 0.8 0.8 0.8 0.7 0.8 1.0 0.8 0.8 0.8 1.1 1.1 1.1 1.2 1.4 1.3 1.3 1.3 1.4
Intensity Change (Log2) – – – 0.07 0.04 -0.14 -0.24 -0.24 -0.4 -0.41 -0.42 -0.51 0.68 0.68 0.48 0.65 0.79 0.85 0.62 0.57 0.68


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 1.7 1.3
Cytoplasm -1.0 -0.8 -1.9 -3.0 -3.6 -4.4 -4.3 -5.4 -5.4 -1.1 -0.7 -1.4 -1.8 -1.9 -2.0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 2.8 3.6 3.8 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.0885 7.1718 6.7338 5.5693 5.3045 6.4574 6.0185 8.3459 7.5829 7.09 6.9565 7.3704 3.6597 4.4491 3.592 3.9334 3.903 3.83
Actin 0.057 0.0004 0.0083 0.0005 0.0266 0.0013 0.0172 0.0002 0.0324 0.0032 0.0003 0.0001 0.0008 0.0002 0.0154 0.0002 0.0001 0.0139
Bud 0.0014 0.0001 0.0095 0.0004 0.0005 0.0002 0.002 0.0003 0.0008 0.0003 0.0003 0 0 0.0001 0.0002 0.0002 0.0001 0.0001
Bud Neck 0.0085 0.0001 0.0007 0.0003 0.002 0.0032 0.0015 0.0001 0.0015 0.0008 0.0003 0.0005 0.0001 0.0001 0.0005 0.0002 0.0002 0.0015
Bud Periphery 0.0025 0.0001 0.0022 0.0002 0.0007 0.0002 0.0018 0.0001 0.0008 0.0002 0.0003 0 0 0 0.0002 0 0 0.0001
Bud Site 0.0039 0.0005 0.002 0.0002 0.0022 0.0001 0.0037 0.0008 0.0117 0.002 0.0002 0 0.0001 0.0003 0.0003 0.0001 0 0.0001
Cell Periphery 0.0013 0.0002 0.0003 0.0002 0.0002 0.0001 0.0004 0.0001 0.0002 0.0001 0.0001 0 0.0001 0.0001 0.0001 0 0 0.0001
Cytoplasm 0.7743 0.9892 0.9192 0.9011 0.7571 0.9742 0.8461 0.9907 0.9044 0.9187 0.9079 0.9891 0.9266 0.9959 0.9115 0.9724 0.991 0.9586
Cytoplasmic Foci 0.0183 0.0018 0.0087 0.0078 0.0208 0.0034 0.0211 0.0025 0.0072 0.0175 0.0058 0.0028 0.0035 0.0008 0.0238 0.0083 0.002 0.0041
Eisosomes 0.0006 0 0.0001 0 0.0005 0 0.0001 0 0.0001 0 0 0 0.0001 0 0.0002 0 0 0.0004
Endoplasmic Reticulum 0.0303 0.0019 0.0131 0.0564 0.0046 0.0024 0.0065 0.001 0.0015 0.0025 0.0016 0.0021 0.008 0.0005 0.0023 0.0017 0.0016 0.0088
Endosome 0.0209 0.0013 0.0119 0.0169 0.0295 0.0039 0.0283 0.0012 0.0124 0.0056 0.0402 0.0014 0.0026 0.0003 0.0091 0.0084 0.0017 0.0032
Golgi 0.0114 0.0002 0.0066 0.0007 0.0232 0.0019 0.0115 0.0001 0.0096 0.0058 0.0025 0.0001 0.0003 0.0001 0.0033 0.0004 0.0001 0.0009
Lipid Particles 0.0069 0 0.0028 0.0001 0.0149 0.0011 0.0115 0 0.0033 0.0046 0.0027 0 0.0002 0 0.0022 0 0 0.0004
Mitochondria 0.0287 0.0002 0.003 0.0006 0.0835 0.0041 0.0122 0 0.0038 0.022 0.0039 0.0001 0.0002 0 0.0013 0.0001 0 0.0003
None 0.0096 0.0019 0.0029 0.0029 0.0035 0.001 0.01 0.0014 0.0025 0.0017 0.002 0.0029 0.051 0.0012 0.0227 0.0023 0.0019 0.0056
Nuclear Periphery 0.0042 0.0002 0.001 0.0034 0.0107 0.0002 0.0022 0.0002 0.0018 0.0005 0.009 0.0002 0.0022 0.0001 0.001 0.0005 0.0002 0.0005
Nucleolus 0.0003 0 0.0001 0 0.0006 0 0.0018 0 0.0001 0.0001 0.0011 0 0.0002 0 0.0001 0 0 0
Nucleus 0.0018 0.0006 0.0014 0.0016 0.0015 0.0006 0.0049 0.0005 0.001 0.0008 0.0049 0.0005 0.0018 0.0003 0.0005 0.0008 0.0005 0.0004
Peroxisomes 0.0081 0 0.0029 0.0001 0.0116 0.0005 0.0066 0 0.002 0.012 0.0003 0 0 0 0.0029 0 0 0.0002
Punctate Nuclear 0.0014 0 0.0008 0.0002 0.0026 0.0001 0.0019 0.0001 0.0019 0.0002 0.001 0 0.0013 0 0.0005 0.0002 0 0.0002
Vacuole 0.0073 0.0011 0.002 0.0053 0.0015 0.0012 0.0062 0.0006 0.0008 0.0011 0.0088 0.0003 0.0007 0.0002 0.0015 0.0039 0.0003 0.0005
Vacuole Periphery 0.0014 0.0001 0.0004 0.001 0.0019 0.0003 0.0024 0.0001 0.0003 0.0003 0.0068 0.0001 0.0001 0 0.0004 0.0004 0.0001 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 25.8138 22.5985 22.4375 30.4207 22.3894 23.8423 28.7225 27.2596 26.1342 32.218
Translational Efficiency 0.8948 1.0184 0.8696 0.6095 0.7682 0.7751 0.6683 0.672 0.7257 0.5807

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1223 1017 258 601 742 970 133 659 1965 1987 391 1260

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 753.05 959.09 1327.26 1145.09 829.42 914.60 1223.21 1137.88 781.89 937.37 1291.87 1141.32
Standard Deviation 114.01 125.39 157.75 166.64 120.18 124.01 182.12 149.86 122.12 126.69 173.59 158.13
Intensity Change Log 2 — 0.348921 0.817633 0.604643 — 0.141038 0.560497 0.456174 — 0.243704 0.688585 0.528734

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000148 0.000482 0.000365 0.000470 0.000164 0.000276 0.016021 0.002584 0.000154 0.000382 0.005690 0.001576
Bud Neck 0.004470 0.001209 0.001830 0.001685 0.001346 0.002432 0.003114 0.001908 0.003290 0.001806 0.002267 0.001801
Bud Site 0.015979 0.017131 0.008957 0.021494 0.010182 0.022080 0.229518* 0.023297 0.013790 0.019547 0.083981 0.022437
Cell Periphery 0.000195 0.000084 0.000152 0.000089 0.000113 0.000084 0.000242 0.000124 0.000164 0.000084 0.000183 0.000107
Cytoplasm 0.685822* 0.778709* 0.672515* 0.720733* 0.766415* 0.737405* 0.440615* 0.763915* 0.716255* 0.758546* 0.593633* 0.743318*
Cytoplasmic Foci 0.167596 0.124034 0.062397 0.055592 0.137700 0.151791 0.024709 0.059521 0.156307 0.137584 0.049577 0.057647
Eisosomes 0.000052 0.000012 0.000018 0.000011 0.000017 0.000019 0.000042 0.000015 0.000039 0.000015 0.000026 0.000013
Endoplasmic Reticulum 0.001811 0.000677 0.004110 0.000243 0.001054 0.000581 0.001237 0.000518 0.001525 0.000630 0.003133 0.000387
Endosome 0.014112 0.009781 0.011726 0.011343 0.009062 0.011002 0.008645 0.010413 0.012205 0.010377 0.010678 0.010857
Golgi 0.006271 0.006208 0.000819 0.005616 0.003384 0.007008 0.000303 0.006427 0.005181 0.006599 0.000644 0.006040
Lipid Particles 0.002214 0.001614 0.002403 0.001312 0.001287 0.000979 0.001208 0.002437 0.001864 0.001304 0.001996 0.001900
Mitochondria 0.002100 0.001500 0.000034 0.001596 0.000333 0.002315 0.000235 0.003445 0.001433 0.001898 0.000102 0.002563
Mitotic Spindle 0.000195 0.002876 0.001989 0.014860 0.000962 0.005708 0.004943 0.019851 0.000485 0.004259 0.002994 0.017470
None 0.006974 0.002257 0.001684 0.000662 0.001224 0.000889 0.000927 0.000777 0.004803 0.001589 0.001426 0.000722
Nuclear Periphery 0.000114 0.000036 0.000403 0.000077 0.000056 0.000040 0.000262 0.000140 0.000092 0.000038 0.000355 0.000110
Nuclear Periphery Foci 0.000859 0.001258 0.012678 0.001576 0.001762 0.000734 0.003593 0.002298 0.001200 0.001002 0.009588 0.001954
Nucleolus 0.000729 0.000114 0.000271 0.000100 0.000325 0.000352 0.000096 0.000083 0.000576 0.000230 0.000211 0.000091
Nucleus 0.022433 0.006639 0.027348 0.012565 0.012216 0.006620 0.022164 0.007466 0.018575 0.006630 0.025585 0.009898
Peroxisomes 0.001003 0.001247 0.000295 0.001248 0.000687 0.001761 0.003234 0.003121 0.000883 0.001498 0.001295 0.002228
Vacuole 0.062971 0.042019 0.189108 0.144425 0.050022 0.045599 0.236974* 0.087906 0.058082 0.043766 0.205390* 0.114865
Vacuole Periphery 0.003954 0.002113 0.000900 0.004300 0.001686 0.002323 0.001919 0.003755 0.003098 0.002216 0.001247 0.004015

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.93 -5.99 -2.96 0.95 1.32 -2.17 -1.43 -2.32 -2.09 1.15 -2.47 -1.54 -2.62 -1.65 1.05
Bud Neck 6.41 4.60 5.73 -1.91 2.53 -4.09 -4.06 0.03 3.53 3.58 4.36 3.12 5.48 2.18 4.18
Bud Site -0.60 4.20 -0.19 0.31 -3.33 -6.96 -4.47 -3.65 2.45 4.14 -4.47 -3.67 -1.96 1.92 3.27
Cell Periphery 3.65 1.10 3.24 -0.30 3.85 3.06 -1.79 2.37 -0.16 2.63 4.15 0.09 3.76 -0.33 4.92
Cytoplasm -9.50 1.44 0.81 7.90 -0.80 2.65 12.88 4.05 1.69 -10.83 -5.74 8.57 2.18 6.84 -7.07
Cytoplasmic Foci 6.74 17.23 22.88 13.93 3.06 -1.86 18.68 14.52 17.38 -7.12 3.78 23.19 27.22 22.10 0.07
Eisosomes 10.06 7.76 10.40 0.78 4.16 -1.12 -1.81 2.87 4.08 2.62 8.83 4.69 10.53 3.75 4.19
Endoplasmic Reticulum 2.89 -0.99 4.69 3.18 2.02 2.52 0.62 5.47 2.33 4.05 3.57 -0.98 5.80 3.89 2.21
Endosome 3.43 2.37 5.37 2.58 3.42 -1.71 2.97 3.36 4.58 0.56 2.16 3.25 6.90 4.84 3.73
Golgi 0.12 15.72 4.27 2.90 -1.19 -3.37 10.98 1.28 3.61 -2.70 -1.74 18.67 4.63 4.56 -2.51
Lipid Particles 0.99 -0.72 4.10 0.75 3.83 1.26 -2.52 -0.56 -1.15 1.02 1.66 -2.00 1.75 -0.13 2.92
Mitochondria 0.57 3.11 1.89 0.94 -1.07 -2.10 2.27 -1.34 0.67 -1.60 -0.59 3.29 0.63 1.06 -1.95
Mitotic Spindle -2.40 -2.97 -3.13 -1.90 -2.23 -2.51 -2.35 -2.77 -0.69 0.16 -3.50 -3.26 -4.24 -1.80 -1.70
None 2.66 3.30 4.21 1.37 0.70 1.35 1.87 2.95 1.12 0.57 3.05 3.37 4.45 1.58 0.83
Nuclear Periphery 3.69 -3.42 -1.66 -7.63 2.98 0.99 -7.78 -3.31 -4.01 6.76 3.64 -6.34 -2.71 -7.65 5.62
Nuclear Periphery Foci -2.56 -9.38 -6.80 -3.33 8.52 3.79 -4.56 -1.19 -5.08 4.21 1.25 -10.10 -5.65 -6.01 9.20
Nucleolus 4.60 2.78 4.71 1.21 1.19 -0.14 2.23 2.68 1.33 2.22 2.47 3.22 5.42 1.40 1.42
Nucleus 7.41 -1.99 2.23 -7.70 4.98 4.21 -4.43 0.42 -5.05 4.73 8.30 -4.34 2.84 -9.24 7.68
Peroxisomes -1.16 10.00 1.65 2.11 -1.64 -3.86 -0.90 -1.47 0.53 -0.33 -3.59 1.39 -0.63 1.30 -1.34
Vacuole 5.86 -9.29 -15.88 -18.06 -1.10 1.11 -12.22 -15.24 -16.16 7.58 5.32 -14.40 -21.69 -24.18 4.55
Vacuole Periphery 3.09 8.24 2.40 0.58 -0.68 -1.49 1.89 0.42 1.11 -0.67 2.31 7.76 2.53 1.15 -0.97
Standard name
Human Ortholog
Description Component of cytosolic iron-sulfur protein assembly (CIA) machinery; acts at a late step of Fe-S cluster assembly; forms the CIA targeting complex with Cia1p and Cia2p that directs Fe-S cluster incorporation and maturation of a subset of cytosolic and nuclear proteins involved in methionine biosynthesis, DNA replication and repair, transcription, and telomere maintenance; ortholog of human MMS19
Localization
Cell Percentages cytoplasm (99%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-4

Met18

Met18


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Met18-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available