Standard name
Human Ortholog
Description Forkhead family transcription factor; rate-limiting replication origin activator; evolutionarily conserved lifespan regulator; binds multiple chromosomal elements with distinct specificities, cell cycle dynamics; regulates transcription elongation, chromatin silencing at mating loci, expression of G2/M phase genes; facilitates clustering, activation of early-firing replication origins; binds HML recombination enhancer, regulates donor preference during mating-type switching
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0.05 0.05 0 0 0 0.05 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0.11 0.11 0.07 0.2 0.11 0.13 0.1 0.08 0.08 0.11 0.12 0.16 0.19 0.11 0.08 0.1 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0.05 0 0 0 0 0 0 0 0.05 0.08 0.14 0.05 0.06 0 0 0.05 0 0.13 0.19 0.15
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.45 0.32 0.57 0.52 0.44 0.66 0.7 0.61 0.73 0.81 0.77 0.83 0.06 0.06 0 0.1 0.05 0.05 0 0 0 0 0 0
Nucleus 0.71 0.77 0.75 0.53 0.54 0.44 0.42 0.45 0.43 0.39 0.46 0.47 0.71 0.54 0.53 0.66 0.7 0.65 0.61 0.67 0.58 0.3 0.21 0.22
Nuclear Periphery 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.15 0 0.05 0.12 0.1 0.05 0.12 0.11 0.1 0.07 0.08 0.06 0.14 0.22 0.14 0.14 0.1 0.15 0.14 0.08 0.06 0.21 0.18 0.15
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0.07 0.11 0.06 0.12 0.11 0.05 0 0 0 0.05 0.07 0.05 0 0 0 0.1 0.06 0.09 0.2 0.21 0.14
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.06 0.07 0.08 0.06 0.08 0.08 0.08 0.1 0.18
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud 0 0 0 0 1 0 1 6 4 5 2 2 0 0 0 1 0 0 0 3 0 1 2 6
Bud Neck 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 2 3 8
Bud Site 1 0 1 0 1 2 10 13 7 5 3 9 1 0 1 1 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1 9 5 9 10 18 22 36 19 15 12 21 56 112 129 45 24 23 1 4 3 3 2 4
Endoplasmic Reticulum 1 2 0 0 0 0 0 2 0 0 0 0 0 2 0 7 8 4 0 0 0 1 1 2
Endosome 4 0 0 1 7 0 5 3 1 2 0 0 23 57 97 22 18 6 5 13 2 57 122 98
Golgi 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 1 2 1
Mitochondria 57 82 25 42 59 58 139 176 134 146 118 165 28 38 30 40 14 11 1 2 1 9 20 21
Nucleus 90 196 33 43 73 39 84 130 80 70 70 94 337 374 363 273 215 147 82 194 42 139 141 147
Nuclear Periphery 1 3 0 0 0 4 5 1 3 5 4 2 5 16 9 10 10 4 2 1 0 7 15 16
Nucleolus 19 8 2 10 14 4 23 31 19 12 13 11 69 153 98 57 32 34 18 23 4 96 120 99
Peroxisomes 1 0 0 0 0 0 0 0 0 0 0 0 0 6 6 2 0 0 0 0 0 0 0 1
SpindlePole 1 2 0 6 15 5 24 32 9 6 3 2 26 47 37 17 10 5 13 18 6 92 137 94
Vac/Vac Membrane 2 8 0 0 1 0 0 2 3 3 2 2 4 23 43 24 20 17 7 23 5 36 65 119
Unique Cell Count 126 256 44 81 135 88 199 287 184 180 153 199 477 690 691 412 306 225 135 289 74 460 659 655
Labelled Cell Count 178 310 67 111 182 130 313 432 279 269 227 308 549 829 813 499 351 251 135 289 74 460 659 655


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.1 4.5 4.0 4.0 4.1 3.9 3.6 3.8 3.8 3.2 3.5 3.3 5.6 5.3 5.3 6.6 8.2 8.1 5.1 4.8 5.4
Std Deviation (1e-4) 0.6 0.8 0.8 1.1 1.2 1.5 1.4 1.3 1.7 1.1 1.6 1.4 1.1 1.4 1.2 1.5 2.1 1.9 1.5 1.0 1.2
Intensity Change (Log2) – – – 0.02 0.04 -0.04 -0.16 -0.09 -0.06 -0.31 -0.18 -0.29 0.48 0.41 0.41 0.73 1.04 1.02 0.34 0.28 0.44


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.3074 4.9013 5.279 5.078 4.9345 4.9956 1.959 1.7236 1.9798 1.8464 1.7154 1.8704 2.8628 2.7814 3.2518 3.0842 3.0362 2.8208
Actin 0.0147 0 0.01 0.0107 0.0013 0.0002 0.0441 0.0001 0.004 0.0159 0 0.0005 0.0013 0.0001 0.0001 0.0001 0 0
Bud 0.0006 0 0.0002 0.0005 0.0002 0.0001 0.0006 0.0001 0.0007 0.0011 0 0.0002 0.0002 0.0046 0 0 0 0
Bud Neck 0.0022 0.001 0.0018 0.0022 0.003 0.0026 0.0008 0.0009 0.0011 0.0151 0.0004 0.0029 0.0019 0.0005 0.0003 0.0002 0.0017 0.0024
Bud Periphery 0.001 0.0001 0.0006 0.0011 0.0005 0.0003 0.0011 0.0001 0.0014 0.001 0 0.0006 0.0004 0.0005 0.0001 0 0.0001 0.0001
Bud Site 0.0035 0.0004 0.0017 0.0039 0.0021 0.0002 0.0024 0.0005 0.0027 0.0037 0 0.0008 0.0014 0.0014 0.0002 0.0001 0.0001 0.0001
Cell Periphery 0.0003 0 0.0002 0.0002 0.0001 0.0001 0.0002 0.0001 0.0003 0.0003 0 0.0001 0.0001 0.0001 0 0 0 0
Cytoplasm 0.0215 0.0002 0.0009 0.0017 0.0004 0.0001 0.0063 0.0086 0.0037 0.0161 0.0001 0.0009 0.0025 0.0069 0.0003 0.003 0.0001 0.0001
Cytoplasmic Foci 0.0095 0.0001 0.0009 0.0062 0.0015 0.0001 0.0388 0.0008 0.0098 0.0027 0 0.0023 0.002 0.0003 0 0.0004 0 0
Eisosomes 0.0003 0 0.0001 0.0001 0 0 0.0003 0 0.0001 0.0003 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0044 0 0.0003 0.0003 0.0002 0.0003 0.003 0.0011 0.0028 0.0021 0 0.0003 0.0002 0.0004 0.0001 0.0006 0 0
Endosome 0.0224 0.0002 0.0013 0.0166 0.0088 0.0013 0.0249 0.0065 0.0207 0.0084 0 0.0077 0.0027 0.0011 0.0001 0.0036 0 0.0001
Golgi 0.0027 0 0.0007 0.0188 0.0106 0.0001 0.0105 0.0001 0.0072 0.0016 0 0.0014 0.0011 0 0 0 0 0
Lipid Particles 0.0076 0.0002 0.0025 0.0322 0.0016 0.0002 0.0265 0.0011 0.0128 0.0008 0 0.0068 0.0018 0.0002 0 0.0004 0 0.0001
Mitochondria 0.0173 0.0003 0.0036 0.0378 0.0165 0.0011 0.0073 0.0005 0.024 0.0013 0.0001 0.0009 0.0008 0.0007 0.0004 0.0003 0.0002 0.0001
None 0.006 0.0001 0.0005 0.0004 0.0002 0.0001 0.005 0.0077 0.0137 0.0031 0 0.0016 0.0011 0.0091 0.0001 0.0149 0 0.0001
Nuclear Periphery 0.0292 0.0025 0.0157 0.0022 0.0193 0.0091 0.0191 0.0293 0.0083 0.0061 0.0011 0.005 0.0036 0.0168 0.0058 0.0133 0.0015 0.0028
Nucleolus 0.2551 0.2245 0.0866 0.0342 0.1191 0.327 0.1355 0.1134 0.0349 0.0176 0.0426 0.2832 0.3096 0.1419 0.0528 0.0204 0.1377 0.3406
Nucleus 0.5351 0.7244 0.8506 0.8132 0.8021 0.6207 0.5527 0.7943 0.8107 0.8963 0.8939 0.5949 0.6329 0.7776 0.9365 0.9321 0.8441 0.6133
Peroxisomes 0.0066 0 0.0005 0.0045 0.0008 0 0.031 0.0001 0.0115 0.0006 0 0.0033 0.0009 0 0 0 0 0
Punctate Nuclear 0.0507 0.0451 0.0149 0.0107 0.0086 0.0348 0.0852 0.0293 0.0134 0.0036 0.0616 0.0825 0.0345 0.0354 0.0026 0.0086 0.0144 0.0398
Vacuole 0.0065 0.0006 0.0037 0.0017 0.001 0.0007 0.0026 0.0044 0.0134 0.0016 0 0.0035 0.0006 0.002 0.0003 0.0014 0.0001 0.0002
Vacuole Periphery 0.0028 0.0001 0.0027 0.0007 0.002 0.0008 0.0019 0.0011 0.0028 0.0006 0 0.0006 0.0002 0.0005 0.0003 0.0004 0 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 29.955 54.9117 17.1036 16.9647 27.0391 15.9166 45.0681 33.3093 20.8888 30.0771
Translational Efficiency 1.0606 1.0711 1.2092 0.8157 0.9996 1.4555 1.1864 1.0893 1.0692 0.8751

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1898 1400 2271 454 1749 1212 545 2861 3647 2612 2816 3315

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 639.52 807.52 932.92 956.02 699.92 762.48 1002.97 1074.18 668.49 786.62 946.48 1058.00
Standard Deviation 81.45 112.53 117.83 140.42 94.76 109.56 108.20 164.55 93.11 113.41 119.28 166.49
Intensity Change Log 2 — 0.336509 0.544764 0.580051 — 0.123509 0.519017 0.617974 — 0.229135 0.531367 0.599992

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000090 0.000488 0.000569 0.001545 0.000173 0.000328 0.001349 0.000420 0.000130 0.000414 0.000720 0.000574
Bud Neck 0.005140 0.009437 0.045072 0.016962 0.004123 0.013895 0.010629 0.047851 0.004652 0.011505 0.038406 0.043621
Bud Site 0.000961 0.001126 0.001598 0.008765 0.000560 0.001578 0.002713 0.005918 0.000768 0.001336 0.001814 0.006308
Cell Periphery 0.000122 0.000058 0.000057 0.000194 0.000095 0.000083 0.000091 0.000263 0.000109 0.000070 0.000064 0.000253
Cytoplasm 0.056560 0.006096 0.001363 0.013668 0.057709 0.013869 0.010622 0.031403 0.057111 0.009702 0.003155 0.028974
Cytoplasmic Foci 0.015006 0.001511 0.000020 0.006479 0.006893 0.002994 0.000593 0.001775 0.011116 0.002199 0.000131 0.002419
Eisosomes 0.000042 0.000033 0.000028 0.000038 0.000029 0.000032 0.000056 0.000044 0.000036 0.000032 0.000033 0.000044
Endoplasmic Reticulum 0.000802 0.003654 0.003779 0.010352 0.001664 0.003532 0.008600 0.002654 0.001215 0.003598 0.004712 0.003708
Endosome 0.002510 0.001144 0.000327 0.006445 0.001121 0.001433 0.000840 0.000789 0.001844 0.001278 0.000426 0.001564
Golgi 0.000758 0.000827 0.000157 0.003556 0.000356 0.001003 0.000284 0.000261 0.000565 0.000909 0.000182 0.000712
Lipid Particles 0.004331 0.000920 0.000055 0.000472 0.002266 0.001636 0.000543 0.000125 0.003341 0.001252 0.000150 0.000173
Mitochondria 0.004091 0.004712 0.001756 0.004588 0.001393 0.009413 0.001447 0.003794 0.002797 0.006893 0.001697 0.003903
Mitotic Spindle 0.005695 0.014486 0.005039 0.041040 0.002829 0.009466 0.015499 0.019511 0.004321 0.012157 0.007063 0.022459
None 0.031212 0.005398 0.001527 0.009402 0.022394 0.007717 0.007393 0.008909 0.026983 0.006474 0.002662 0.008977
Nuclear Periphery 0.001641 0.003050 0.001118 0.001447 0.001671 0.002832 0.001027 0.002055 0.001655 0.002949 0.001100 0.001972
Nuclear Periphery Foci 0.004471 0.006218 0.000823 0.002048 0.005200 0.004797 0.004793 0.000432 0.004820 0.005558 0.001591 0.000653
Nucleolus 0.212843 0.187776 0.106549 0.053252 0.129732 0.179613 0.090845 0.026635 0.172986 0.183988 0.103510 0.030280
Nucleus 0.649960* 0.750430* 0.828428* 0.794983* 0.759776* 0.739829* 0.835220* 0.841752* 0.702624* 0.745511* 0.829742* 0.835347*
Peroxisomes 0.001745 0.001111 0.000220 0.000616 0.000792 0.001413 0.000612 0.000171 0.001288 0.001251 0.000296 0.000232
Vacuole 0.001410 0.001063 0.001149 0.018043 0.001038 0.003217 0.006634 0.004620 0.001232 0.002063 0.002211 0.006458
Vacuole Periphery 0.000609 0.000460 0.000365 0.006103 0.000184 0.001319 0.000210 0.000617 0.000406 0.000859 0.000335 0.001369

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -13.18 -24.87 -14.95 -11.66 -10.50 -3.24 -16.60 -17.01 -4.49 13.06 -10.39 -26.55 -23.68 -9.54 3.95
Bud Neck -6.35 -25.92 -10.03 -8.28 4.35 -7.20 -11.38 -31.02 -21.76 -17.51 -9.52 -27.94 -32.22 -26.32 -7.29
Bud Site -0.78 -3.47 -8.57 -8.57 -7.69 -3.06 -7.38 -9.50 -5.51 0.17 -2.75 -8.21 -10.92 -9.07 -6.79
Cell Periphery 4.65 5.08 -2.57 -6.28 -6.57 1.15 -1.35 -1.72 -2.14 -1.29 4.34 5.27 -1.68 -3.14 -3.35
Cytoplasm 16.36 18.50 12.28 -3.40 -6.30 11.72 13.30 10.50 -2.97 -5.42 19.76 24.57 14.73 -6.76 -14.13
Cytoplasmic Foci 11.05 12.18 9.87 -0.47 -2.35 4.08 9.13 8.59 4.06 -1.56 11.46 15.13 13.91 3.83 -4.02
Eisosomes 5.17 9.74 -3.39 -6.05 -7.62 0.96 -8.86 0.73 0.36 6.65 4.46 1.23 1.09 -0.35 0.69
Endoplasmic Reticulum -13.35 -22.23 -9.77 -6.67 -6.58 -5.88 -15.41 -11.62 0.88 12.94 -13.01 -23.35 -17.37 -2.46 4.73
Endosome 3.99 7.06 -0.03 -2.53 -4.05 0.31 -0.02 4.70 2.81 6.01 3.40 7.97 5.60 1.91 -2.92
Golgi 0.38 4.35 -0.81 -0.96 -2.10 -1.34 -1.02 1.95 1.86 4.41 -0.86 4.57 2.06 2.10 -1.87
Lipid Particles 8.15 12.06 10.52 2.15 -7.85 1.94 4.73 6.86 4.81 7.10 7.21 13.12 13.24 6.15 0.97
Mitochondria -0.51 3.52 0.67 1.03 -2.27 -5.22 -2.70 -8.21 3.69 -6.43 -4.48 2.63 -2.23 3.58 -6.63
Mitotic Spindle -4.12 -2.17 -6.45 -4.89 -5.88 -3.32 -5.66 -9.81 -5.75 0.35 -5.30 -5.33 -11.40 -6.56 -6.72
None 13.98 16.21 11.59 -2.27 -6.17 10.00 10.96 10.96 0.55 -1.18 17.20 21.21 16.38 -1.51 -10.33
Nuclear Periphery -3.10 1.17 -4.42 0.87 -6.18 -3.46 -1.42 -5.67 -0.51 -4.87 -4.45 0.96 -6.81 0.06 -8.01
Nuclear Periphery Foci -1.51 6.15 0.19 1.12 -2.46 2.54 -0.19 7.78 4.16 4.65 0.77 6.10 8.72 6.50 2.54
Nucleolus 5.35 13.48 16.85 11.99 6.87 -2.05 3.41 14.47 15.45 6.69 2.53 11.99 25.58 21.90 13.34
Nucleus -11.72 -19.37 -9.39 -1.13 2.48 -1.23 -2.81 -9.04 -6.84 -3.26 -9.30 -17.04 -18.19 -7.68 -0.82
Peroxisomes 3.25 7.64 5.08 1.46 -4.78 -1.31 0.94 7.01 3.95 7.80 1.27 8.61 9.61 5.44 4.58
Vacuole 0.50 0.06 -5.97 -6.01 -5.97 -3.28 -6.69 -6.68 -1.68 4.64 -2.83 -5.90 -8.76 -6.57 -4.09
Vacuole Periphery 0.47 1.03 -1.46 -1.58 -1.69 -2.81 -5.78 -2.49 2.21 -1.03 -2.20 0.34 -1.34 0.89 -1.81
Standard name
Human Ortholog
Description Forkhead family transcription factor; rate-limiting replication origin activator; evolutionarily conserved lifespan regulator; binds multiple chromosomal elements with distinct specificities, cell cycle dynamics; regulates transcription elongation, chromatin silencing at mating loci, expression of G2/M phase genes; facilitates clustering, activation of early-firing replication origins; binds HML recombination enhancer, regulates donor preference during mating-type switching
Localization
Cell Percentages nucleus (75%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Fkh1

Fkh1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Fkh1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available