Standard name
Human Ortholog
Description Component of Shu complex (aka PCSS complex); Shu complex also includes Psy3, Shu1, Shu2, and promotes error-free DNA repair,; Shu complex mediates inhibition of Srs2p function; promotes formation of Rad51p filaments; Psy3p and Csm2p contain similar DNA-binding regions which work together to form a single DNA binding site; required for accurate chromosome segregation during meiosis
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.93 0.93 0.96 0.98 0.96 0.9 0.82 – 0.76 0.76 0.57 0.71 0.93 0.95 0.93 0.62 0.58 0.6 0.72 0.77 0.63 0.77 0.77 0.8
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.07 0.05 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.06 0 0 0 0.16 0.15 – 0.29 0.28 0.57 0.37 0 0 0 0.1 0 0.08 0.11 0.05 0.19 0.07 0.08 0.05
Nucleus 0 0 0 0 0 0.05 0 – 0 0 0 0 0 0 0 0.12 0.09 0.1 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.09 0 0 0 0 0 0 – 0.05 0 0 0 0 0 0.05 0.26 0.29 0.32 0.06 0 0.05 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 1 1 0 3 3 1
Bud 0 0 0 0 0 0 0 – 1 1 4 3 2 0 0 0 0 0 0 2 0 2 7 4
Bud Neck 0 1 0 0 1 0 2 – 2 2 2 2 1 1 0 0 2 0 1 2 0 0 1 2
Bud Site 0 0 0 0 0 0 0 – 1 0 3 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 1 0 0 0 1 – 0 0 0 0 0 0 1 0 0 0 0 0 0 1 1 1
Cytoplasm 261 195 25 117 210 95 178 – 157 173 64 136 125 311 255 83 84 83 218 177 41 180 196 208
Endoplasmic Reticulum 0 2 0 0 0 0 0 – 0 0 0 0 3 2 0 9 7 6 1 0 0 2 0 1
Endosome 2 0 0 1 0 0 3 – 0 0 1 2 0 3 6 1 2 5 8 5 0 2 1 1
Golgi 1 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 1 0 0 2 0 1 1 2
Mitochondria 9 12 0 1 2 17 32 – 59 64 64 70 2 7 1 14 6 11 32 12 12 16 19 13
Nucleus 1 0 1 0 1 5 7 – 5 3 2 3 0 3 1 16 13 14 7 3 0 2 1 2
Nuclear Periphery 0 0 0 0 0 0 1 – 1 1 0 0 0 0 1 0 0 0 0 0 0 0 0 1
Nucleolus 0 2 0 0 0 0 1 – 0 2 1 0 1 0 0 0 0 0 0 1 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 1 0 0 0 0 0 0 0 0 0 1 0 0 0 0
SpindlePole 0 0 0 1 2 0 5 – 2 3 2 5 1 1 1 0 0 0 4 6 0 2 6 8
Vac/Vac Membrane 25 6 0 3 9 2 9 – 10 5 1 3 5 8 13 35 42 44 19 8 3 10 7 5
Unique Cell Count 280 209 26 119 218 106 217 206 228 113 191 135 329 273 134 144 139 305 231 66 233 254 262
Labelled Cell Count 299 218 27 123 225 119 239 238 255 144 225 140 336 279 158 157 163 305 231 66 233 254 262


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.6 4.0 4.6 5.1 5.6 3.4 4.6 – 3.7 4.3 3.9 4.2 4.3 4.2 4.6 5.0 5.3 5.4 4.1 4.2 4.2
Std Deviation (1e-4) 0.5 0.9 0.5 2.2 2.5 1.1 2.4 – 1.3 2.5 2.0 5.5 1.1 1.6 1.3 1.0 1.1 1.5 1.8 1.7 1.7
Intensity Change (Log2) – – – 0.15 0.28 -0.42 -0.02 – -0.31 -0.1 -0.24 -0.14 -0.09 -0.15 -0.01 0.1 0.19 0.23 -0.19 -0.15 -0.15


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 2.9 3.2 3.4

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -1.6802 -1.155 -1.4846 -1.6337 -1.9524 -1.4576 -0.1094 -0.1676 -0.1859 0.0519 0.0752 -0.3208 1.7889 1.4949 1.4158 1.3912 1.5159 1.5426
Actin 0.004 0.0005 0.0068 0.0005 0.0007 0.0008 0.0289 0.0004 0.0041 0.006 0.0145 0.008 0.045 0.0001 0.0132 0.0056 0.009 0.0073
Bud 0.0025 0.0001 0.0021 0 0 0.0005 0.0014 0.0005 0.0006 0.0005 0.0059 0.0005 0.0012 0 0.0003 0.0001 0.001 0.0003
Bud Neck 0.0004 0.0008 0.0005 0.0002 0.0002 0.0015 0.0016 0.0002 0.0007 0.0009 0.0007 0.0027 0.0171 0 0.0014 0.0005 0.0002 0.0051
Bud Periphery 0.0004 0.0001 0.0007 0 0 0.0003 0.0033 0.0007 0.0017 0.0007 0.014 0.0019 0.0029 0 0.0008 0.0001 0.0009 0.0004
Bud Site 0.0008 0.0143 0.0023 0.0001 0.0002 0.0006 0.0075 0.0011 0.001 0.0014 0.0024 0.0003 0.0139 0.0001 0.0086 0.0017 0.01 0.0016
Cell Periphery 0.0001 0.0001 0.0001 0 0 0.0001 0.0004 0.0001 0.0002 0.0001 0.0002 0.0001 0.0022 0 0.0004 0.0001 0.0001 0
Cytoplasm 0.0715 0.071 0.0833 0.105 0.0333 0.142 0.1074 0.2242 0.131 0.2283 0.1288 0.2499 0.0592 0.0613 0.0964 0.2158 0.0444 0.1176
Cytoplasmic Foci 0.0184 0.0089 0.0111 0.0054 0.0112 0.0169 0.0235 0.0131 0.0194 0.0144 0.0465 0.0115 0.0258 0.0019 0.0071 0.0145 0.0216 0.0059
Eisosomes 0.0006 0.0003 0.0004 0.0001 0.0001 0.0001 0.0006 0.0001 0.0001 0.0001 0.0003 0.0001 0.001 0.0001 0.0004 0.0001 0.0002 0.0001
Endoplasmic Reticulum 0.0019 0.0011 0.002 0.0019 0.0006 0.0012 0.0047 0.002 0.0031 0.0018 0.0023 0.002 0.0117 0.0004 0.0015 0.002 0.001 0.001
Endosome 0.0114 0.0024 0.0045 0.0024 0.0018 0.0037 0.0314 0.0048 0.0222 0.0162 0.0485 0.0091 0.0153 0.0002 0.005 0.0025 0.0074 0.0024
Golgi 0.0013 0.0002 0.0004 0.0001 0.001 0.0006 0.0137 0.0005 0.0066 0.0181 0.0287 0.0023 0.0056 0 0.0061 0.0017 0.0051 0.0004
Lipid Particles 0.0126 0.001 0.0019 0.0007 0.0104 0.0024 0.0239 0.0011 0.0101 0.0451 0.0095 0.0023 0.0088 0.0001 0.0026 0.0057 0.0018 0.0004
Mitochondria 0.0026 0.0003 0.0006 0.0006 0.0013 0.0006 0.0187 0.0005 0.0138 0.0379 0.0391 0.0077 0.0046 0.0001 0.016 0.0018 0.0014 0.0005
None 0.8225 0.8724 0.8222 0.7828 0.8573 0.744 0.6632 0.6728 0.699 0.4988 0.5885 0.5883 0.7332 0.9345 0.8266 0.6973 0.822 0.8352
Nuclear Periphery 0.0037 0.0012 0.0057 0.0053 0.0015 0.0045 0.0087 0.0046 0.0037 0.0037 0.0044 0.005 0.0088 0.0002 0.0014 0.0019 0.0021 0.0012
Nucleolus 0.0033 0.0006 0.0015 0.0013 0.0007 0.0017 0.001 0.0007 0.0009 0.0007 0.0012 0.0014 0.0017 0.0001 0.0004 0.0003 0.0003 0.0004
Nucleus 0.022 0.019 0.0317 0.0791 0.0495 0.0543 0.0404 0.056 0.0561 0.0918 0.0117 0.0906 0.0168 0.0004 0.0043 0.0288 0.0627 0.0079
Peroxisomes 0.0028 0.0004 0.0005 0.0003 0.026 0.0012 0.0096 0.0088 0.0045 0.0274 0.0285 0.0023 0.014 0 0.0042 0.0164 0.0056 0.0092
Punctate Nuclear 0.0148 0.0043 0.0202 0.0129 0.004 0.0224 0.0056 0.0061 0.0159 0.0037 0.0116 0.0122 0.0064 0.0005 0.0018 0.0018 0.0026 0.0024
Vacuole 0.0018 0.001 0.0011 0.0009 0.0002 0.0006 0.0031 0.0014 0.0042 0.0018 0.009 0.0013 0.0032 0.0001 0.001 0.0012 0.0005 0.0007
Vacuole Periphery 0.0008 0.0001 0.0003 0.0003 0.0001 0.0002 0.0016 0.0002 0.0012 0.0008 0.0037 0.0006 0.0016 0 0.0004 0.0002 0.0002 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 8.5935 10.7549 2.2802 3.397 2.2284 8.5887 3.8837 6.8151 3.2838 6.2135
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
192 1251 1492 2063 1807 2146 131 1411 1999 3397 1623 3474

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 634.21 653.66 809.35 753.01 635.30 731.39 887.16 831.15 635.20 702.76 815.63 784.75
Standard Deviation 88.33 129.49 91.40 97.93 71.56 90.65 91.62 116.23 73.34 113.01 93.84 112.49
Intensity Change Log 2 — 0.043580 0.351803 0.247708 — 0.203203 0.481756 0.387671 — 0.125666 0.418298 0.319446

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000101 0.000270 0.000446 0.000396 0.000056 0.000828 0.000665 0.000424 0.000060 0.000622 0.000464 0.000407
Bud Neck 0.015306 0.021939 0.004883 0.010562 0.010163 0.038203 0.008914 0.004805 0.010657 0.032213 0.005208 0.008224
Bud Site 0.004219 0.010536 0.004712 0.019870 0.001417 0.017072 0.002793 0.018065 0.001687 0.014665 0.004557 0.019137
Cell Periphery 0.000654 0.000151 0.000093 0.000228 0.000123 0.000261 0.000168 0.000195 0.000174 0.000220 0.000099 0.000215
Cytoplasm 0.347492* 0.425992* 0.148629* 0.416884* 0.447564* 0.415597* 0.043260 0.406681* 0.437953* 0.419425* 0.140124* 0.412740*
Cytoplasmic Foci 0.170585 0.197109* 0.002364 0.009067 0.172246 0.167550 0.003321 0.012146 0.172087 0.178436 0.002441 0.010318
Eisosomes 0.000296 0.000098 0.000020 0.000030 0.000125 0.000150 0.000077 0.000044 0.000142 0.000131 0.000024 0.000035
Endoplasmic Reticulum 0.004129 0.001141 0.003095 0.001699 0.000708 0.000673 0.009295 0.002195 0.001037 0.000845 0.003595 0.001901
Endosome 0.010814 0.014165 0.000750 0.001987 0.005264 0.011913 0.002340 0.003721 0.005797 0.012742 0.000878 0.002691
Golgi 0.002881 0.002474 0.000038 0.000191 0.000962 0.003898 0.000103 0.001736 0.001147 0.003374 0.000044 0.000819
Lipid Particles 0.017320 0.009960 0.000514 0.000853 0.010510 0.006817 0.002400 0.001333 0.011164 0.007974 0.000667 0.001048
Mitochondria 0.011574 0.011266 0.000727 0.001407 0.005912 0.015783 0.000590 0.001559 0.006456 0.014120 0.000716 0.001469
Mitotic Spindle 0.000769 0.005107 0.001223 0.014618 0.000230 0.010728 0.000389 0.019099 0.000282 0.008658 0.001156 0.016438
None 0.007936 0.013780 0.004282 0.014994 0.004527 0.006644 0.002519 0.014620 0.004855 0.009272 0.004140 0.014842
Nuclear Periphery 0.001632 0.000994 0.001573 0.001924 0.000746 0.000614 0.001520 0.001628 0.000831 0.000754 0.001568 0.001803
Nuclear Periphery Foci 0.000909 0.000715 0.000326 0.000722 0.000304 0.000311 0.001016 0.000895 0.000362 0.000460 0.000382 0.000793
Nucleolus 0.002930 0.004889 0.000513 0.001203 0.001219 0.002893 0.002784 0.000819 0.001383 0.003628 0.000696 0.001047
Nucleus 0.357263* 0.219280* 0.813129* 0.462704* 0.311133* 0.245524* 0.883154* 0.444324* 0.315563* 0.235859* 0.818781* 0.455239*
Peroxisomes 0.008892 0.009739 0.000224 0.000310 0.004545 0.013074 0.000435 0.000662 0.004963 0.011846 0.000241 0.000453
Vacuole 0.032392 0.048280 0.012337 0.039943 0.021558 0.039983 0.033965 0.062356 0.022599 0.043038 0.014083 0.049046
Vacuole Periphery 0.001906 0.002117 0.000122 0.000409 0.000687 0.001485 0.000293 0.002692 0.000804 0.001718 0.000135 0.001336

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.46 -1.66 -2.82 -0.84 0.33 -2.53 -7.89 -6.67 1.36 3.87 -2.92 -2.20 -5.46 1.20 0.46
Bud Neck -1.59 2.27 1.25 7.01 -3.85 -12.53 0.75 3.39 15.50 0.14 -12.64 4.00 1.99 16.06 -3.02
Bud Site -3.18 -0.35 -5.12 -2.09 -5.28 -10.72 -1.61 -6.31 2.34 -4.69 -12.07 -2.90 -10.27 0.65 -5.93
Cell Periphery 5.76 6.35 5.51 -1.04 -4.14 -7.06 -2.94 -2.35 5.19 1.48 -2.86 5.18 0.92 3.79 -4.43
Cytoplasm -3.50 10.19 0.13 7.65 -26.05 3.73 40.81 13.67 10.40 -25.81 2.62 40.83 13.91 12.61 -30.71
Cytoplasmic Foci -1.81 13.08 12.57 32.08 -8.87 0.90 36.21 34.78 37.14 -6.08 -1.03 39.24 37.39 49.16 -12.30
Eisosomes 6.23 8.61 8.11 15.17 -8.21 -2.15 1.91 7.50 11.62 3.52 1.13 13.10 11.11 16.39 -8.90
Endoplasmic Reticulum 1.24 -0.06 0.44 -11.40 4.38 0.26 -9.55 -15.91 -17.26 6.90 0.71 -11.16 -8.90 -21.40 5.57
Endosome -1.10 3.35 3.01 10.43 -4.76 -6.93 2.83 4.02 11.27 -0.15 -7.93 8.00 5.88 15.84 -6.13
Golgi 0.32 1.69 1.63 5.54 -3.48 -5.59 6.99 0.36 4.19 -1.23 -5.36 5.54 2.55 7.06 -1.59
Lipid Particles 3.03 7.24 7.05 10.57 -6.25 4.01 7.88 10.49 13.28 2.63 3.66 12.87 12.34 16.91 -5.67
Mitochondria 0.09 2.41 2.30 6.54 -3.11 -5.62 5.03 4.46 10.35 -3.57 -5.14 5.42 4.97 12.22 -3.49
Mitotic Spindle -2.75 -1.11 -7.72 -4.37 -7.36 -6.72 -0.57 -6.91 -2.51 -6.86 -7.40 -2.68 -10.71 -4.21 -9.61
None -2.53 2.43 -1.33 1.65 -5.25 -2.28 4.48 -3.85 -2.01 -5.87 -4.64 1.78 -5.53 -0.56 -6.69
Nuclear Periphery 0.79 -0.43 -2.26 -13.33 -9.44 1.33 -7.14 -14.22 -15.96 -5.19 0.62 -9.07 -17.13 -22.16 -11.07
Nuclear Periphery Foci 0.30 0.91 -0.71 -2.20 -3.76 0.05 -4.21 -4.12 -4.16 -0.31 -1.31 -2.38 -5.63 -5.19 -4.54
Nucleolus -2.69 3.98 2.33 5.72 -3.68 -4.04 -0.94 0.46 4.03 1.05 -6.52 5.62 0.60 6.57 -3.80
Nucleus 6.07 -20.65 -6.27 -29.45 35.67 8.25 -31.96 -17.53 -24.92 20.60 11.17 -63.39 -23.15 -38.80 43.16
Peroxisomes -0.58 6.08 6.05 11.54 -0.51 -7.81 9.42 9.15 12.62 -0.28 -8.50 11.21 11.06 16.54 -1.12
Vacuole -2.72 2.21 -8.11 -9.13 -18.63 -9.29 -4.42 -20.13 -15.88 -5.95 -11.86 -0.02 -25.86 -18.31 -24.64
Vacuole Periphery -0.35 4.16 3.76 7.00 -4.53 -4.42 3.23 -0.75 0.46 -1.38 -5.44 6.84 0.52 3.52 -1.94
Standard name
Human Ortholog
Description Component of Shu complex (aka PCSS complex); Shu complex also includes Psy3, Shu1, Shu2, and promotes error-free DNA repair,; Shu complex mediates inhibition of Srs2p function; promotes formation of Rad51p filaments; Psy3p and Csm2p contain similar DNA-binding regions which work together to form a single DNA binding site; required for accurate chromosome segregation during meiosis
Localization
Cell Percentages cytoplasm (18%), nucleus (6%), mixed (48%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Csm2

Csm2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Csm2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available