Standard name | |
---|---|
Human Ortholog | |
Description | Regulator of septin dynamics; involved in the regulation of septin dynamics at bud neck after mitotic entry, likely by stabilizing septin structure; regulated at post-translational level by cell cycle dependent phosphorylation; likely phosphorylated by Cdc28p and dephosphorylated by Cdc14p before cytokinesis; high-copy suppressor of synthetic lethality of sis2 sit4 double mutant; VHS2 has a paralog, MLF3, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 3 | 0 | 2 | 8 |
Bud | 1 | 6 | 2 | 1 | 0 | 8 | 25 | 18 | 12 | 25 | 21 | 19 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 5 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 3 | 0 | 1 | 4 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 14 | 64 | 96 | 76 | 70 | 111 | 118 | 117 | 150 | 120 | 132 | 181 | 19 | 38 | 64 | 62 | 51 | 89 | 1 | 7 | 21 | 1 | 6 | 42 |
Cytoplasm | 104 | 477 | 542 | 459 | 511 | 742 | 896 | 875 | 670 | 696 | 645 | 715 | 256 | 565 | 570 | 504 | 323 | 401 | 96 | 452 | 483 | 390 | 476 | 601 |
Endoplasmic Reticulum | 0 | 3 | 0 | 2 | 2 | 13 | 2 | 5 | 16 | 8 | 6 | 4 | 2 | 3 | 3 | 20 | 4 | 12 | 4 | 3 | 5 | 0 | 2 | 3 |
Endosome | 0 | 0 | 2 | 0 | 2 | 0 | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 4 | 1 | 0 | 0 | 3 | 3 | 0 | 2 | 6 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 3 | 1 | 0 | 3 |
Mitochondria | 0 | 2 | 4 | 12 | 40 | 144 | 196 | 142 | 149 | 219 | 206 | 205 | 1 | 0 | 1 | 4 | 5 | 2 | 0 | 3 | 4 | 3 | 3 | 4 |
Nucleus | 0 | 2 | 0 | 0 | 1 | 6 | 9 | 6 | 3 | 5 | 3 | 4 | 1 | 6 | 6 | 16 | 2 | 1 | 0 | 0 | 0 | 1 | 0 | 0 |
Nuclear Periphery | 1 | 1 | 0 | 0 | 1 | 3 | 3 | 7 | 1 | 4 | 2 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 2 | 1 |
Nucleolus | 0 | 0 | 0 | 1 | 1 | 0 | 3 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Peroxisomes | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 |
Vac/Vac Membrane | 1 | 0 | 4 | 4 | 2 | 7 | 33 | 33 | 28 | 33 | 31 | 38 | 2 | 4 | 1 | 10 | 11 | 8 | 1 | 4 | 1 | 2 | 1 | 0 |
Unique Cell Count | 108 | 484 | 549 | 471 | 538 | 842 | 1031 | 1000 | 816 | 871 | 836 | 899 | 259 | 572 | 574 | 525 | 336 | 413 | 110 | 486 | 544 | 406 | 508 | 690 |
Labelled Cell Count | 122 | 555 | 650 | 556 | 630 | 1035 | 1287 | 1206 | 1031 | 1111 | 1049 | 1170 | 281 | 616 | 645 | 621 | 397 | 514 | 110 | 486 | 544 | 406 | 508 | 690 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.2 | 7.3 | 6.8 | 6.6 | 6.8 | 6.1 | 6.2 | 6.5 | 6.2 | 6.1 | 6.1 | 6.3 | 8.5 | 8.8 | 8.4 | 9.8 | 9.1 | 9.6 | 9.0 | 8.9 | 9.3 |
Std Deviation (1e-4) | 0.8 | 1.0 | 0.9 | 1.3 | 1.3 | 1.2 | 1.2 | 1.4 | 1.1 | 1.4 | 1.3 | 1.4 | 1.1 | 1.0 | 1.0 | 1.5 | 1.6 | 1.5 | 1.4 | 1.2 | 1.3 |
Intensity Change (Log2) | – | – | – | -0.04 | -0.01 | -0.16 | -0.15 | -0.07 | -0.14 | -0.16 | -0.17 | -0.12 | 0.31 | 0.36 | 0.3 | 0.51 | 0.41 | 0.49 | 0.4 | 0.39 | 0.44 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 3.0 | 2.4 | 2.0 | 3.4 | 3.1 | 2.7 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -0.6 | -2.1 | -2.2 | -3.3 | -3.2 | 0.4 | -1.9 | -0.8 | 1.2 | -3.9 | -5.6 | -3.0 | -2.6 | -0.9 | 1.6 |
Cytoplasm | -1.5 | -3.5 | -7.3 | -7.8 | -7.6 | -9.5 | -10.3 | -11.2 | -10.5 | 0.1 | 0.1 | 1.0 | -2.8 | -2.5 | -1.8 |
Endoplasmic Reticulum | 0 | 0 | 2.9 | 0 | 0 | 3.3 | 0 | 0 | 0 | 0 | 0 | 0 | 4.6 | 0 | 4.0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 2.3 | 5.6 | 9.7 | 10.4 | 8.7 | 10.1 | 12.3 | 12.1 | 11.6 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4.1 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 3.1 | 3.2 | 3.2 | 3.5 | 3.5 | 3.8 | 0 | 0 | 0 | 1.7 | 2.8 | 1.7 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 6.0552 | 6.6134 | 6.4235 | 5.6945 | 5.7654 | 6.6835 | 4.8938 | 6.0207 | 5.4699 | 5.1426 | 5.1696 | 5.8046 | 4.4367 | 4.8477 | 4.8914 | 4.8096 | 3.727 | 4.7327 |
Actin | 0.0247 | 0.0081 | 0.0189 | 0.0197 | 0.0092 | 0.0043 | 0.0342 | 0.0018 | 0.0241 | 0.0099 | 0.0229 | 0.0066 | 0.0319 | 0.0032 | 0.0143 | 0.0911 | 0.023 | 0.0106 |
Bud | 0.0006 | 0.0002 | 0.0004 | 0.0034 | 0.0002 | 0.0013 | 0.0012 | 0.0082 | 0.0039 | 0.0022 | 0.0014 | 0.0016 | 0.0005 | 0.0007 | 0.0091 | 0.0007 | 0.0011 | 0.0047 |
Bud Neck | 0.0126 | 0.0007 | 0.0021 | 0.0017 | 0.0019 | 0.0029 | 0.0071 | 0.0006 | 0.0031 | 0.0015 | 0.0208 | 0.0098 | 0.0151 | 0.0019 | 0.002 | 0.0017 | 0.0023 | 0.014 |
Bud Periphery | 0.0012 | 0.0002 | 0.0008 | 0.0022 | 0.0003 | 0.0013 | 0.0016 | 0.0014 | 0.003 | 0.0034 | 0.0039 | 0.0018 | 0.0013 | 0.0007 | 0.0046 | 0.0016 | 0.0023 | 0.0037 |
Bud Site | 0.003 | 0.013 | 0.0087 | 0.0046 | 0.0006 | 0.0009 | 0.0156 | 0.0282 | 0.0262 | 0.0015 | 0.0035 | 0.0008 | 0.0094 | 0.0357 | 0.0095 | 0.0046 | 0.0094 | 0.0011 |
Cell Periphery | 0.0146 | 0.0056 | 0.0086 | 0.0057 | 0.0014 | 0.0062 | 0.0054 | 0.0044 | 0.0042 | 0.0011 | 0.0013 | 0.0021 | 0.0047 | 0.0059 | 0.0053 | 0.0033 | 0.0014 | 0.0041 |
Cytoplasm | 0.7779 | 0.9269 | 0.9075 | 0.9067 | 0.823 | 0.9434 | 0.7629 | 0.9457 | 0.88 | 0.9215 | 0.7979 | 0.9263 | 0.8268 | 0.9185 | 0.8998 | 0.8035 | 0.9113 | 0.9233 |
Cytoplasmic Foci | 0.0108 | 0.0028 | 0.01 | 0.007 | 0.0103 | 0.0027 | 0.0205 | 0.0008 | 0.0139 | 0.0154 | 0.0096 | 0.0086 | 0.0115 | 0.0012 | 0.0057 | 0.0062 | 0.0033 | 0.003 |
Eisosomes | 0.0017 | 0.0001 | 0.0002 | 0.0001 | 0.0006 | 0 | 0.0006 | 0 | 0.0001 | 0 | 0.0003 | 0.0001 | 0.0004 | 0.0002 | 0.0001 | 0.0004 | 0.0007 | 0 |
Endoplasmic Reticulum | 0.0093 | 0.0019 | 0.0061 | 0.0034 | 0.0064 | 0.0027 | 0.0082 | 0.0021 | 0.0027 | 0.0032 | 0.0047 | 0.0029 | 0.0108 | 0.002 | 0.0038 | 0.0034 | 0.0031 | 0.0032 |
Endosome | 0.0127 | 0.002 | 0.0026 | 0.0036 | 0.0127 | 0.0003 | 0.0344 | 0.0001 | 0.0048 | 0.0015 | 0.0223 | 0.0057 | 0.0161 | 0.0002 | 0.001 | 0.0212 | 0.0052 | 0.0022 |
Golgi | 0.0063 | 0.0006 | 0.0026 | 0.0026 | 0.0025 | 0.0002 | 0.0049 | 0.0001 | 0.0039 | 0.0013 | 0.0156 | 0.002 | 0.005 | 0.0002 | 0.001 | 0.0072 | 0.0035 | 0.0009 |
Lipid Particles | 0.0052 | 0.002 | 0.0014 | 0.0043 | 0.0043 | 0.0007 | 0.0104 | 0.0002 | 0.0029 | 0.0015 | 0.0175 | 0.0011 | 0.016 | 0.0001 | 0.0004 | 0.0087 | 0.0111 | 0.0005 |
Mitochondria | 0.0038 | 0.0007 | 0.0007 | 0.0005 | 0.053 | 0.0001 | 0.0025 | 0 | 0.0011 | 0.0002 | 0.0078 | 0.001 | 0.0033 | 0 | 0.0003 | 0.0073 | 0.0046 | 0.0004 |
None | 0.0871 | 0.0309 | 0.0228 | 0.0117 | 0.0424 | 0.031 | 0.0271 | 0.0048 | 0.0064 | 0.0147 | 0.0132 | 0.0163 | 0.0241 | 0.0282 | 0.0366 | 0.0193 | 0.0035 | 0.0236 |
Nuclear Periphery | 0.0068 | 0.001 | 0.0006 | 0.0017 | 0.0063 | 0.0002 | 0.0149 | 0.0001 | 0.0007 | 0.0007 | 0.0206 | 0.0018 | 0.0085 | 0.0002 | 0.0004 | 0.0009 | 0.0031 | 0.0007 |
Nucleolus | 0.0005 | 0.0004 | 0.0001 | 0.001 | 0.0018 | 0 | 0.0013 | 0.0001 | 0.0006 | 0.0001 | 0.001 | 0.0003 | 0.0006 | 0 | 0.0002 | 0.0012 | 0.0016 | 0.0001 |
Nucleus | 0.0018 | 0.0007 | 0.0005 | 0.0048 | 0.0074 | 0.0004 | 0.0201 | 0.0007 | 0.0015 | 0.0009 | 0.0148 | 0.0033 | 0.0021 | 0.0005 | 0.0012 | 0.001 | 0.0014 | 0.001 |
Peroxisomes | 0.012 | 0.0006 | 0.0036 | 0.0033 | 0.0017 | 0.0001 | 0.0085 | 0 | 0.0114 | 0.0155 | 0.0084 | 0.0042 | 0.0035 | 0 | 0.0029 | 0.0032 | 0.0031 | 0.0003 |
Punctate Nuclear | 0.0057 | 0.0009 | 0.0012 | 0.0107 | 0.0128 | 0.0005 | 0.0143 | 0.0002 | 0.0047 | 0.0019 | 0.005 | 0.0028 | 0.0067 | 0.0002 | 0.001 | 0.0012 | 0.0029 | 0.0018 |
Vacuole | 0.0012 | 0.0005 | 0.0006 | 0.0011 | 0.0007 | 0.0005 | 0.0032 | 0.0003 | 0.0006 | 0.0017 | 0.0056 | 0.0007 | 0.0013 | 0.0004 | 0.0007 | 0.0098 | 0.0014 | 0.0007 |
Vacuole Periphery | 0.0005 | 0.0001 | 0.0001 | 0.0002 | 0.0003 | 0 | 0.0011 | 0 | 0.0001 | 0.0001 | 0.0019 | 0.0002 | 0.0005 | 0 | 0 | 0.0025 | 0.0007 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 15.1497 | 24.0763 | 13.9575 | 17.467 | 14.1861 | 19.3473 | 20.9202 | 23.8752 | 17.2869 | 21.6125 |
Translational Efficiency | 1.9219 | 1.0864 | 1.5554 | 0.9838 | 1.86 | 1.8482 | 1.4748 | 1.4863 | 1.8987 | 1.4462 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1698 | 785 | 2609 | 153 | 1819 | 1288 | 1655 | 287 | 3517 | 2073 | 4264 | 440 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 772.32 | 809.97 | 981.00 | 1142.02 | 856.53 | 889.32 | 1027.91 | 1103.39 | 815.87 | 859.27 | 999.21 | 1116.82 |
Standard Deviation | 99.79 | 141.85 | 119.18 | 136.78 | 111.85 | 109.01 | 135.36 | 142.91 | 114.23 | 128.39 | 127.77 | 142.00 |
Intensity Change Log 2 | — | 0.068670 | 0.345054 | 0.564317 | — | 0.054199 | 0.263138 | 0.365367 | — | 0.061078 | 0.302559 | 0.463125 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.003319 | 0.002840 | 0.001185 | 0.006498 | 0.002096 | 0.004757 | 0.002123 | 0.001967 | 0.002686 | 0.004031 | 0.001549 | 0.003543 |
Bud Neck | 0.026400 | 0.009368 | 0.009389 | 0.006911 | 0.013811 | 0.018851 | 0.005698 | 0.007306 | 0.019889 | 0.015260 | 0.007956 | 0.007168 |
Bud Site | 0.040592 | 0.051437 | 0.024561 | 0.075964 | 0.027026 | 0.038311 | 0.030516 | 0.034318 | 0.033576 | 0.043282 | 0.026872 | 0.048799 |
Cell Periphery | 0.004013 | 0.001107 | 0.001619 | 0.002875 | 0.002853 | 0.002203 | 0.001037 | 0.000289 | 0.003413 | 0.001788 | 0.001393 | 0.001188 |
Cytoplasm | 0.636461* | 0.616502* | 0.647881* | 0.319776* | 0.665787* | 0.612709* | 0.593949* | 0.436198* | 0.651628* | 0.614145* | 0.626948* | 0.395715* |
Cytoplasmic Foci | 0.134486 | 0.149567 | 0.019717 | 0.056960 | 0.112236 | 0.119760 | 0.035120 | 0.049352 | 0.122978 | 0.131047 | 0.025696 | 0.051998 |
Eisosomes | 0.006391 | 0.000550 | 0.000477 | 0.000485 | 0.002398 | 0.002670 | 0.000266 | 0.000088 | 0.004326 | 0.001867 | 0.000395 | 0.000226 |
Endoplasmic Reticulum | 0.004412 | 0.002568 | 0.002743 | 0.005619 | 0.003162 | 0.002206 | 0.003133 | 0.001343 | 0.003766 | 0.002343 | 0.002894 | 0.002830 |
Endosome | 0.005240 | 0.013950 | 0.001132 | 0.003619 | 0.003307 | 0.006539 | 0.001955 | 0.021043 | 0.004240 | 0.009345 | 0.001452 | 0.014984 |
Golgi | 0.012873 | 0.004600 | 0.000231 | 0.000306 | 0.004202 | 0.005576 | 0.000254 | 0.010147 | 0.008389 | 0.005206 | 0.000240 | 0.006725 |
Lipid Particles | 0.005654 | 0.003369 | 0.001041 | 0.004859 | 0.003391 | 0.003711 | 0.001561 | 0.002244 | 0.004483 | 0.003581 | 0.001242 | 0.003153 |
Mitochondria | 0.003087 | 0.000930 | 0.000222 | 0.000122 | 0.000982 | 0.001865 | 0.000407 | 0.015819 | 0.001998 | 0.001511 | 0.000294 | 0.010361 |
Mitotic Spindle | 0.001442 | 0.000634 | 0.002408 | 0.001598 | 0.000453 | 0.004305 | 0.009773 | 0.035070 | 0.000931 | 0.002915 | 0.005267 | 0.023431 |
None | 0.002468 | 0.002992 | 0.001951 | 0.000768 | 0.004506 | 0.001439 | 0.002113 | 0.000639 | 0.003522 | 0.002027 | 0.002014 | 0.000684 |
Nuclear Periphery | 0.000122 | 0.000103 | 0.000262 | 0.000260 | 0.000080 | 0.000104 | 0.000308 | 0.002618 | 0.000100 | 0.000104 | 0.000280 | 0.001798 |
Nuclear Periphery Foci | 0.001127 | 0.002156 | 0.001244 | 0.009714 | 0.001713 | 0.001609 | 0.003363 | 0.009024 | 0.001430 | 0.001816 | 0.002066 | 0.009264 |
Nucleolus | 0.000322 | 0.001281 | 0.000080 | 0.000038 | 0.000269 | 0.000261 | 0.000075 | 0.000231 | 0.000294 | 0.000647 | 0.000078 | 0.000164 |
Nucleus | 0.060391 | 0.035412 | 0.165771* | 0.043991 | 0.093046 | 0.065753 | 0.120967 | 0.015201 | 0.077280 | 0.054264 | 0.148381* | 0.025212 |
Peroxisomes | 0.004280 | 0.003496 | 0.000235 | 0.005289 | 0.001597 | 0.003753 | 0.000750 | 0.003712 | 0.002892 | 0.003656 | 0.000435 | 0.004260 |
Vacuole | 0.046005 | 0.096038 | 0.117772 | 0.454254* | 0.056748 | 0.102799 | 0.186486 | 0.333150* | 0.051561 | 0.100239 | 0.144442 | 0.375261* |
Vacuole Periphery | 0.000915 | 0.001097 | 0.000079 | 0.000092 | 0.000336 | 0.000818 | 0.000146 | 0.020242 | 0.000616 | 0.000924 | 0.000105 | 0.013235 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 1.14 | 9.59 | 0.03 | -0.70 | -3.53 | -2.74 | 1.10 | 3.91 | 4.21 | 3.76 | -2.11 | 6.57 | 3.38 | 3.63 | -1.15 |
Bud Neck | 10.94 | 12.95 | 15.53 | 3.49 | 4.19 | -3.05 | 8.54 | 9.40 | 9.94 | 2.60 | 4.09 | 14.68 | 17.36 | 10.43 | 5.24 |
Bud Site | -2.41 | 8.40 | -0.94 | 0.46 | -3.82 | -4.14 | 1.13 | 1.83 | 4.50 | 1.05 | -4.01 | 7.06 | 1.08 | 3.46 | -2.34 |
Cell Periphery | 10.66 | 12.37 | 7.10 | -0.84 | -0.18 | 2.66 | 10.32 | 13.65 | 12.35 | 12.05 | 8.92 | 15.20 | 15.70 | 6.86 | 4.00 |
Cytoplasm | 2.37 | 6.01 | 17.01 | 14.91 | 14.95 | 7.35 | 19.25 | 15.26 | 11.90 | 6.64 | 6.70 | 17.08 | 21.35 | 17.80 | 14.87 |
Cytoplasmic Foci | -2.54 | 37.03 | 15.34 | 14.51 | -5.20 | -1.66 | 27.92 | 18.91 | 17.92 | -1.86 | -2.23 | 46.76 | 25.33 | 23.12 | -5.97 |
Eisosomes | 12.32 | 12.90 | 13.19 | 2.44 | 2.68 | -0.84 | 15.31 | 16.42 | 10.10 | 10.07 | 8.60 | 16.73 | 17.58 | 10.33 | 7.29 |
Endoplasmic Reticulum | 2.09 | 2.27 | 1.60 | -0.80 | -0.67 | 1.65 | 0.01 | 5.30 | 4.13 | 12.34 | 2.80 | 1.94 | 5.04 | 2.23 | 6.24 |
Endosome | -6.03 | 12.92 | 7.82 | 8.08 | -3.66 | -5.73 | 8.53 | -1.43 | 0.68 | -2.52 | -7.77 | 15.10 | -0.11 | 4.27 | -3.05 |
Golgi | 8.20 | 21.28 | 21.44 | 5.08 | 0.14 | -1.56 | 19.43 | 1.70 | 2.32 | -1.56 | 4.93 | 25.90 | 8.13 | 3.69 | -1.55 |
Lipid Particles | 4.86 | 18.31 | 4.87 | -0.44 | -6.67 | -0.88 | 9.59 | 8.06 | 6.44 | 1.99 | 3.28 | 20.84 | 10.09 | 4.67 | -3.67 |
Mitochondria | 3.71 | 5.36 | 5.62 | 3.28 | 5.39 | -1.12 | 3.26 | -0.94 | -0.55 | -1.23 | 0.95 | 6.25 | -0.07 | -0.39 | -1.21 |
Mitotic Spindle | 1.44 | -1.46 | -1.58 | -1.77 | -1.31 | -2.82 | -4.66 | -2.39 | -1.28 | -0.44 | -2.24 | -4.81 | -2.82 | -2.06 | -1.53 |
None | -0.68 | 1.73 | 4.97 | 3.18 | 1.93 | 5.29 | 4.72 | 5.88 | 1.21 | 2.81 | 3.83 | 4.61 | 7.33 | 3.22 | 2.67 |
Nuclear Periphery | 0.32 | -6.02 | -5.19 | -6.85 | -0.65 | -0.99 | -18.15 | -2.00 | -1.93 | -0.88 | -0.16 | -13.33 | -2.42 | -2.41 | -1.00 |
Nuclear Periphery Foci | -4.09 | -1.23 | -6.66 | -5.36 | -6.50 | 0.81 | -9.56 | -1.60 | -1.69 | -0.10 | -3.04 | -5.45 | -4.04 | -3.51 | -3.11 |
Nucleolus | -1.55 | 5.77 | 6.52 | 1.99 | 3.10 | 0.34 | 7.04 | 4.63 | 3.29 | -0.60 | -1.47 | 8.75 | 7.20 | 2.40 | 0.23 |
Nucleus | 7.83 | -29.07 | 1.78 | -1.93 | 16.91 | 8.18 | -9.35 | 18.04 | 10.96 | 24.27 | 9.79 | -26.87 | 13.19 | 5.96 | 32.18 |
Peroxisomes | 1.67 | 9.21 | 2.37 | 1.58 | -1.22 | -3.91 | 7.00 | 1.58 | 4.20 | -1.36 | -1.50 | 11.35 | 3.48 | 4.14 | -1.92 |
Vacuole | -11.81 | -29.21 | -20.36 | -17.82 | -15.09 | -12.18 | -32.76 | -23.37 | -20.42 | -11.41 | -17.30 | -42.42 | -30.88 | -26.92 | -19.81 |
Vacuole Periphery | -0.39 | 7.95 | 8.13 | 2.79 | 0.36 | -1.32 | 4.31 | -1.52 | -1.32 | -1.62 | -1.08 | 9.13 | -1.28 | -1.09 | -1.62 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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