Standard name | |
---|---|
Human Ortholog | |
Description | Peptidyl-prolyl cis/trans-isomerase; activator of the phosphotyrosyl phosphatase activity of PP2A; involved in G1 phase progression, microtubule dynamics, bud morphogenesis and DNA repair; required for rapid reduction of Sgs1p levels in response to rapamycin; subunit of the Tap42p-Sit4p-Rrd1p complex; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 1 | 0 | 1 | 6 | 15 | 7 | 7 | 16 | 4 | 14 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 4 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 |
Bud Site | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 1 | 0 | 2 | 1 | 4 | 4 | 3 | 5 | 3 | 4 | 10 | 0 | 3 | 3 | 4 | 5 | 3 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 337 | 376 | 389 | 327 | 387 | 441 | 471 | 455 | 337 | 368 | 285 | 317 | 415 | 444 | 458 | 435 | 497 | 361 | 329 | 371 | 390 | 336 | 249 | 317 |
Endoplasmic Reticulum | 0 | 2 | 3 | 0 | 1 | 1 | 4 | 4 | 1 | 5 | 5 | 1 | 1 | 2 | 2 | 13 | 14 | 5 | 1 | 0 | 1 | 0 | 1 | 1 |
Endosome | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 2 | 1 | 0 | 0 | 1 | 2 | 2 | 2 | 1 | 1 | 4 | 2 | 0 | 1 | 0 | 5 |
Golgi | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 3 | 0 | 3 | 0 | 0 | 1 |
Mitochondria | 8 | 6 | 3 | 14 | 33 | 152 | 205 | 202 | 180 | 253 | 225 | 292 | 0 | 0 | 0 | 1 | 9 | 2 | 5 | 2 | 1 | 0 | 1 | 2 |
Nucleus | 4 | 7 | 2 | 3 | 5 | 11 | 18 | 19 | 23 | 29 | 19 | 29 | 5 | 3 | 6 | 25 | 17 | 7 | 3 | 8 | 2 | 6 | 3 | 5 |
Nuclear Periphery | 0 | 0 | 0 | 1 | 0 | 1 | 6 | 4 | 8 | 1 | 0 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
SpindlePole | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Vac/Vac Membrane | 6 | 1 | 28 | 25 | 54 | 45 | 111 | 117 | 105 | 120 | 84 | 190 | 12 | 32 | 14 | 49 | 61 | 66 | 2 | 8 | 6 | 4 | 5 | 7 |
Unique Cell Count | 347 | 384 | 411 | 352 | 453 | 566 | 691 | 714 | 558 | 652 | 525 | 679 | 423 | 460 | 467 | 473 | 540 | 397 | 354 | 400 | 415 | 353 | 264 | 356 |
Labelled Cell Count | 357 | 393 | 426 | 372 | 485 | 662 | 835 | 813 | 668 | 798 | 626 | 857 | 434 | 487 | 485 | 531 | 604 | 445 | 354 | 400 | 415 | 353 | 264 | 356 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.0 | 6.7 | 6.6 | 6.2 | 6.3 | 5.7 | 5.7 | 5.6 | 5.7 | 5.4 | 5.2 | 5.3 | 8.5 | 8.8 | 8.2 | 10.2 | 8.9 | 8.7 | 8.7 | 9.1 | 9.6 |
Std Deviation (1e-4) | 0.9 | 0.9 | 1.0 | 0.9 | 1.4 | 1.1 | 1.2 | 1.1 | 1.1 | 1.0 | 1.0 | 1.1 | 1.2 | 1.1 | 1.0 | 1.7 | 1.4 | 1.5 | 1.2 | 1.4 | 1.6 |
Intensity Change (Log2) | – | – | – | -0.08 | -0.06 | -0.22 | -0.22 | -0.23 | -0.2 | -0.28 | -0.35 | -0.32 | 0.38 | 0.42 | 0.32 | 0.63 | 0.43 | 0.4 | 0.4 | 0.46 | 0.55 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 2.6 | 0 | 0 | 2.8 | 0 | 2.5 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -1.0 | -4.5 | -7.2 | -10.3 | -11.5 | -12.2 | -13.4 | -13.6 | -16.1 | 2.7 | 1.4 | 2.7 | -1.6 | -1.6 | -2.0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.2 | 2.1 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 3.0 | 4.8 | 11.0 | 11.9 | 11.5 | 12.4 | 14.1 | 14.9 | 15.2 | 0 | 0 | 0 | 0 | 1.3 | 0 |
Nucleus | 0 | 0 | 2.0 | 2.5 | 2.6 | 3.5 | 3.7 | 3.2 | 3.6 | 0 | 0 | 0 | 4.1 | 2.9 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0.2 | 2.6 | 0.7 | 4.5 | 4.6 | 5.4 | 5.3 | 4.3 | 8.5 | -2.7 | 0.1 | -2.6 | 1.9 | 2.4 | 4.3 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 4.9933 | 5.2293 | 5.1455 | 4.9406 | 4.6478 | 5.1555 | 3.7832 | 4.4785 | 4.1005 | 3.3889 | 4.2729 | 4.1087 | 3.482 | 3.5329 | 3.2895 | 3.3096 | 2.7492 | 3.2945 |
Actin | 0.0012 | 0.0007 | 0.0006 | 0.0005 | 0.0004 | 0.0047 | 0.0461 | 0.005 | 0.0262 | 0.0201 | 0.0043 | 0.0121 | 0.0431 | 0.001 | 0.0054 | 0.0399 | 0.0405 | 0.0075 |
Bud | 0.0044 | 0.0045 | 0.0008 | 0.0004 | 0.0002 | 0.0008 | 0.0015 | 0.0006 | 0.0014 | 0.0014 | 0.0012 | 0.0014 | 0.0048 | 0.0047 | 0.0007 | 0.0052 | 0.0005 | 0.0007 |
Bud Neck | 0.0031 | 0.0012 | 0.0008 | 0.001 | 0.0013 | 0.0016 | 0.0075 | 0.0005 | 0.0012 | 0.0063 | 0.0034 | 0.0033 | 0.001 | 0.0004 | 0.0009 | 0.0046 | 0.0011 | 0.0059 |
Bud Periphery | 0.0012 | 0.0014 | 0.0002 | 0.0002 | 0.0001 | 0.0003 | 0.002 | 0.0002 | 0.0009 | 0.0016 | 0.002 | 0.0013 | 0.0107 | 0.0014 | 0.0007 | 0.004 | 0.0004 | 0.0011 |
Bud Site | 0.0014 | 0.0082 | 0.0042 | 0.0005 | 0.0002 | 0.0003 | 0.0202 | 0.0105 | 0.0137 | 0.0066 | 0.0003 | 0.0059 | 0.0034 | 0.0095 | 0.0068 | 0.0056 | 0.0028 | 0.0024 |
Cell Periphery | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0.0013 | 0.0002 | 0.0002 | 0.0009 | 0.0002 | 0.0002 | 0.0003 | 0.0001 | 0.0002 | 0.0007 | 0.0001 | 0.0001 |
Cytoplasm | 0.793 | 0.8162 | 0.7954 | 0.7062 | 0.7917 | 0.7595 | 0.6798 | 0.9133 | 0.8252 | 0.819 | 0.7248 | 0.8077 | 0.7135 | 0.9149 | 0.8519 | 0.8111 | 0.6851 | 0.8343 |
Cytoplasmic Foci | 0.011 | 0.0109 | 0.0106 | 0.0061 | 0.0104 | 0.011 | 0.0385 | 0.0049 | 0.0154 | 0.0245 | 0.0048 | 0.0259 | 0.0274 | 0.0162 | 0.0149 | 0.0224 | 0.032 | 0.0159 |
Eisosomes | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0.0005 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0 | 0 | 0.0001 | 0.0003 | 0 |
Endoplasmic Reticulum | 0.0041 | 0.0035 | 0.0033 | 0.0021 | 0.0035 | 0.0024 | 0.0176 | 0.0034 | 0.0077 | 0.0098 | 0.0047 | 0.0035 | 0.0133 | 0.0029 | 0.0047 | 0.003 | 0.0027 | 0.0051 |
Endosome | 0.0073 | 0.0056 | 0.004 | 0.0073 | 0.0059 | 0.0054 | 0.0274 | 0.0026 | 0.0311 | 0.0238 | 0.0398 | 0.0194 | 0.0284 | 0.0023 | 0.0142 | 0.0104 | 0.0154 | 0.0106 |
Golgi | 0.0004 | 0.0003 | 0.0002 | 0.0005 | 0.0001 | 0.0009 | 0.0115 | 0.0006 | 0.022 | 0.0057 | 0.0114 | 0.0093 | 0.0134 | 0.0002 | 0.0019 | 0.0029 | 0.0156 | 0.0018 |
Lipid Particles | 0.0002 | 0.0001 | 0.0001 | 0.001 | 0.0001 | 0.0001 | 0.0189 | 0.0005 | 0.0027 | 0.0031 | 0.0028 | 0.0099 | 0.0078 | 0.0008 | 0.001 | 0.0044 | 0.0375 | 0.0014 |
Mitochondria | 0.0048 | 0.0007 | 0.0007 | 0.0033 | 0.0006 | 0.0009 | 0.0039 | 0.0004 | 0.0045 | 0.0037 | 0.0526 | 0.0165 | 0.011 | 0.0004 | 0.0068 | 0.0031 | 0.0194 | 0.0018 |
None | 0.0861 | 0.0462 | 0.0952 | 0.1643 | 0.1325 | 0.0829 | 0.0386 | 0.0282 | 0.0083 | 0.0062 | 0.0042 | 0.0104 | 0.0378 | 0.0141 | 0.0346 | 0.0095 | 0.0078 | 0.0083 |
Nuclear Periphery | 0.0061 | 0.0057 | 0.0057 | 0.0049 | 0.0049 | 0.0048 | 0.012 | 0.003 | 0.0045 | 0.0114 | 0.0314 | 0.0074 | 0.0199 | 0.0035 | 0.0048 | 0.0057 | 0.0043 | 0.0101 |
Nucleolus | 0.0012 | 0.0006 | 0.0004 | 0.0061 | 0.0002 | 0.0002 | 0.0016 | 0.0001 | 0.0007 | 0.0003 | 0.0007 | 0.0009 | 0.0003 | 0.0007 | 0.0005 | 0.0047 | 0.0009 | 0.0005 |
Nucleus | 0.0645 | 0.0817 | 0.0659 | 0.0881 | 0.0369 | 0.1088 | 0.0462 | 0.0213 | 0.024 | 0.0402 | 0.0802 | 0.0502 | 0.0389 | 0.0194 | 0.0333 | 0.0296 | 0.0448 | 0.0782 |
Peroxisomes | 0.0005 | 0.0005 | 0.0005 | 0.0004 | 0.0003 | 0.0007 | 0.0119 | 0.0011 | 0.0026 | 0.0035 | 0.0002 | 0.0035 | 0.009 | 0.0008 | 0.0034 | 0.0016 | 0.0689 | 0.0013 |
Punctate Nuclear | 0.007 | 0.0094 | 0.0099 | 0.0038 | 0.0083 | 0.0133 | 0.0073 | 0.0022 | 0.0037 | 0.0082 | 0.0017 | 0.0083 | 0.0125 | 0.0053 | 0.0057 | 0.0224 | 0.0183 | 0.0101 |
Vacuole | 0.0019 | 0.0021 | 0.0011 | 0.0025 | 0.002 | 0.0012 | 0.0046 | 0.0012 | 0.0031 | 0.0026 | 0.0096 | 0.0021 | 0.0023 | 0.0012 | 0.0063 | 0.0082 | 0.0012 | 0.0025 |
Vacuole Periphery | 0.0004 | 0.0003 | 0.0002 | 0.0009 | 0.0004 | 0.0002 | 0.0011 | 0.0002 | 0.0007 | 0.0008 | 0.0196 | 0.0008 | 0.0012 | 0.0002 | 0.0013 | 0.001 | 0.0004 | 0.0006 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 34.5397 | 35.0489 | 26.0078 | 25.9989 | 28.4428 | 41.0515 | 35.227 | 43.5653 | 40.1307 | 28.289 |
Translational Efficiency | 0.4574 | 0.352 | 0.5184 | 0.6229 | 0.4074 | 0.3361 | 0.374 | 0.4158 | 0.4713 | 0.5155 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1567 | 205 | 1578 | 1656 | 502 | 2047 | 443 | 306 | 2069 | 2252 | 2021 | 1962 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 826.51 | 948.03 | 969.78 | 1021.48 | 854.76 | 871.05 | 1150.41 | 1126.89 | 833.36 | 878.06 | 1009.37 | 1037.92 |
Standard Deviation | 103.66 | 126.11 | 158.26 | 136.54 | 105.97 | 113.75 | 136.92 | 175.40 | 104.93 | 117.05 | 171.03 | 148.31 |
Intensity Change Log 2 | — | 0.197900 | 0.230625 | 0.305557 | — | 0.027236 | 0.428557 | 0.398755 | — | 0.113658 | 0.334642 | 0.353691 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000171 | 0.000253 | 0.000248 | 0.000975 | 0.000186 | 0.000232 | 0.000611 | 0.000363 | 0.000174 | 0.000234 | 0.000328 | 0.000879 |
Bud Neck | 0.001798 | 0.002326 | 0.006204 | 0.007756 | 0.000961 | 0.003994 | 0.002329 | 0.002757 | 0.001595 | 0.003842 | 0.005355 | 0.006976 |
Bud Site | 0.003175 | 0.006126 | 0.003600 | 0.021397 | 0.002130 | 0.004538 | 0.003688 | 0.006874 | 0.002922 | 0.004683 | 0.003619 | 0.019132 |
Cell Periphery | 0.000097 | 0.000106 | 0.001585 | 0.000133 | 0.000144 | 0.000070 | 0.000210 | 0.000138 | 0.000108 | 0.000074 | 0.001283 | 0.000134 |
Cytoplasm | 0.666642* | 0.652943* | 0.456588* | 0.533855* | 0.648797* | 0.654823* | 0.347079* | 0.537549* | 0.662312* | 0.654651* | 0.432584* | 0.534431* |
Cytoplasmic Foci | 0.085677 | 0.079074 | 0.015449 | 0.009306 | 0.072200 | 0.061473 | 0.009558 | 0.006393 | 0.082408 | 0.063075 | 0.014158 | 0.008852 |
Eisosomes | 0.000035 | 0.000034 | 0.001535 | 0.000023 | 0.000035 | 0.000031 | 0.000066 | 0.000024 | 0.000035 | 0.000031 | 0.001213 | 0.000023 |
Endoplasmic Reticulum | 0.001385 | 0.001945 | 0.001590 | 0.002541 | 0.001911 | 0.000806 | 0.006830 | 0.001775 | 0.001513 | 0.000910 | 0.002738 | 0.002421 |
Endosome | 0.003608 | 0.005663 | 0.001613 | 0.004805 | 0.003434 | 0.004779 | 0.003213 | 0.001248 | 0.003566 | 0.004859 | 0.001963 | 0.004251 |
Golgi | 0.000807 | 0.000323 | 0.000224 | 0.004455 | 0.000546 | 0.001203 | 0.000120 | 0.000106 | 0.000743 | 0.001123 | 0.000201 | 0.003776 |
Lipid Particles | 0.001801 | 0.001287 | 0.001132 | 0.000941 | 0.001723 | 0.001322 | 0.001867 | 0.000976 | 0.001782 | 0.001318 | 0.001293 | 0.000946 |
Mitochondria | 0.001521 | 0.004447 | 0.000713 | 0.005436 | 0.000238 | 0.004462 | 0.000360 | 0.000284 | 0.001210 | 0.004461 | 0.000635 | 0.004633 |
Mitotic Spindle | 0.002162 | 0.007181 | 0.003922 | 0.025840 | 0.000322 | 0.010148 | 0.000593 | 0.077466 | 0.001715 | 0.009878 | 0.003192 | 0.033892 |
None | 0.002744 | 0.003339 | 0.010880 | 0.006849 | 0.002701 | 0.003644 | 0.005462 | 0.013781 | 0.002733 | 0.003617 | 0.009692 | 0.007930 |
Nuclear Periphery | 0.000343 | 0.000386 | 0.000999 | 0.000921 | 0.000387 | 0.000544 | 0.001593 | 0.000850 | 0.000354 | 0.000529 | 0.001129 | 0.000910 |
Nuclear Periphery Foci | 0.001694 | 0.004376 | 0.000971 | 0.002209 | 0.003878 | 0.000647 | 0.004852 | 0.003570 | 0.002224 | 0.000986 | 0.001821 | 0.002421 |
Nucleolus | 0.000330 | 0.000411 | 0.000590 | 0.000283 | 0.000354 | 0.000806 | 0.000411 | 0.000132 | 0.000335 | 0.000770 | 0.000551 | 0.000259 |
Nucleus | 0.170990* | 0.138318* | 0.436002* | 0.271416* | 0.223224* | 0.174796* | 0.474007* | 0.202437* | 0.183664* | 0.171476* | 0.444333* | 0.260658* |
Peroxisomes | 0.000631 | 0.000687 | 0.000729 | 0.001008 | 0.000599 | 0.001967 | 0.000192 | 0.000195 | 0.000624 | 0.001850 | 0.000611 | 0.000881 |
Vacuole | 0.053827 | 0.089865 | 0.055170 | 0.098114 | 0.035926 | 0.068722 | 0.136825 | 0.143003 | 0.049483 | 0.070647 | 0.073069 | 0.105115 |
Vacuole Periphery | 0.000563 | 0.000908 | 0.000257 | 0.001737 | 0.000303 | 0.000993 | 0.000135 | 0.000079 | 0.000500 | 0.000986 | 0.000230 | 0.001478 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -2.25 | -2.62 | -3.86 | -2.85 | -1.75 | -0.49 | -9.24 | -6.89 | -3.38 | 4.64 | -0.89 | -4.64 | -4.31 | -3.23 | -1.07 |
Bud Neck | -1.50 | -3.95 | -6.46 | -3.18 | 0.45 | -5.35 | -8.18 | -4.48 | -0.80 | -1.66 | -4.27 | -4.69 | -7.81 | -1.59 | 0.02 |
Bud Site | -2.43 | -0.92 | -5.06 | -1.68 | -4.46 | -3.28 | -5.59 | -5.00 | -2.95 | -2.73 | -2.28 | -1.91 | -6.04 | -4.25 | -4.89 |
Cell Periphery | -0.64 | -4.41 | -2.19 | -0.71 | 4.32 | 5.49 | -3.56 | -0.51 | -7.77 | 3.52 | 5.43 | -4.50 | -1.53 | -7.27 | 4.43 |
Cytoplasm | 2.41 | 30.33 | 29.09 | 10.42 | -2.92 | -0.18 | 26.12 | 16.74 | 19.33 | -4.88 | 2.02 | 37.97 | 33.15 | 29.93 | -5.48 |
Cytoplasmic Foci | 0.73 | 24.86 | 26.54 | 9.42 | 3.76 | 2.11 | 13.86 | 13.73 | 22.10 | -0.44 | 5.88 | 28.34 | 29.90 | 25.51 | 3.49 |
Eisosomes | -0.74 | -3.22 | 3.37 | 2.48 | 3.25 | 1.62 | -8.16 | -1.86 | -4.49 | 7.29 | 1.79 | -3.27 | 2.57 | 0.42 | 3.29 |
Endoplasmic Reticulum | -2.27 | -7.02 | -5.93 | -0.26 | 2.09 | 5.78 | -10.98 | -0.58 | -7.55 | 10.91 | 5.48 | -12.07 | -5.38 | -11.18 | 8.45 |
Endosome | -2.48 | 5.98 | 4.68 | 4.79 | 0.18 | -2.11 | 0.25 | 5.26 | 8.17 | 6.43 | -2.74 | 5.68 | 5.61 | 7.19 | 1.55 |
Golgi | 1.82 | 2.36 | -0.34 | -1.31 | -1.59 | -1.32 | 1.12 | 1.15 | 3.76 | 0.58 | -1.01 | 2.60 | -0.25 | 0.41 | -1.59 |
Lipid Particles | 1.23 | 1.83 | 1.40 | -0.09 | -1.81 | 1.45 | -1.00 | -0.66 | -4.04 | 0.61 | 1.67 | 1.49 | 1.36 | -0.94 | -0.49 |
Mitochondria | -0.61 | 1.38 | -0.32 | 0.55 | -1.87 | -4.16 | -2.19 | -1.69 | 4.03 | 1.04 | -3.00 | 1.24 | -0.58 | 2.66 | -1.92 |
Mitotic Spindle | -0.79 | -2.22 | -6.42 | -2.01 | -5.04 | -5.78 | -2.72 | -4.15 | -2.59 | -4.04 | -4.72 | -2.45 | -7.75 | -3.34 | -6.51 |
None | -0.67 | -7.07 | -9.56 | -5.80 | 2.23 | -1.41 | -2.75 | -5.59 | -4.35 | -4.38 | -1.44 | -7.41 | -11.30 | -5.42 | 0.67 |
Nuclear Periphery | -2.11 | -20.31 | -23.12 | -16.00 | -0.51 | -1.41 | -13.68 | -10.73 | -10.96 | 0.14 | -4.02 | -24.02 | -24.56 | -17.74 | -0.09 |
Nuclear Periphery Foci | -3.68 | 2.67 | -2.09 | 3.21 | -4.47 | 2.71 | -0.84 | 0.38 | -8.51 | 2.58 | 3.72 | 0.38 | -0.02 | -8.10 | -0.71 |
Nucleolus | -1.44 | -1.61 | 1.23 | 2.06 | 1.98 | -2.28 | 0.05 | 1.34 | 2.69 | 1.56 | -2.48 | -1.57 | 1.63 | 2.76 | 2.13 |
Nucleus | 2.05 | -36.32 | -20.23 | -13.25 | 18.18 | 4.74 | -15.81 | 0.15 | -3.98 | 15.05 | 1.77 | -39.13 | -17.87 | -19.60 | 22.29 |
Peroxisomes | -0.94 | 0.61 | 2.15 | 2.40 | 0.70 | -3.05 | 1.62 | 1.57 | 4.73 | 0.03 | -3.52 | 1.00 | 2.42 | 4.46 | 0.66 |
Vacuole | -4.90 | -7.50 | -23.85 | -7.40 | -15.76 | -7.61 | -14.99 | -18.33 | -16.39 | -6.49 | -7.52 | -14.30 | -29.34 | -23.93 | -15.22 |
Vacuole Periphery | -0.47 | 2.22 | 0.61 | 0.72 | -1.54 | -2.84 | 2.49 | 3.51 | 3.98 | 6.34 | -1.89 | 2.50 | 0.72 | 2.38 | -1.50 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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