Standard name | |
---|---|
Human Ortholog | |
Description | Protein of unknown function; overexpression causes cell cycle delay or arrest; green fluorescent protein (GFP)-fusion protein localizes to vacuole; null mutant displays elevated frequency of mitochondrial genome loss; relocalizes from nucleus to cytoplasm upon DNA replication stress; AIM20 has a paralog, SKG1, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 2 | 3 | 3 | 2 | 0 | 3 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 5 | 1 | 2 | 2 | 5 |
Bud | 2 | 2 | 1 | 1 | 0 | 4 | 0 | – | 4 | 0 | 6 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 12 | 9 | 3 | 7 | 16 | 22 |
Bud Neck | 0 | 0 | 1 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 3 | 5 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 1 | 0 | – | 0 | 1 | 1 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Cytoplasm | 60 | 108 | 126 | 122 | 110 | 81 | 210 | – | 352 | 391 | 551 | 452 | 311 | 580 | 610 | 64 | 53 | 57 | 3 | 17 | 6 | 40 | 152 | 194 |
Endoplasmic Reticulum | 9 | 11 | 2 | 0 | 0 | 1 | 0 | – | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 13 | 15 | 18 | 5 | 3 | 1 | 4 | 5 | 6 |
Endosome | 43 | 19 | 22 | 12 | 6 | 4 | 4 | – | 3 | 3 | 6 | 1 | 2 | 2 | 2 | 14 | 13 | 5 | 38 | 38 | 19 | 16 | 6 | 18 |
Golgi | 0 | 2 | 0 | 0 | 2 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 3 | 4 | 1 | 2 | 3 | 0 | 2 | 1 | 1 |
Mitochondria | 48 | 43 | 4 | 7 | 2 | 53 | 10 | – | 33 | 24 | 39 | 32 | 0 | 0 | 0 | 6 | 5 | 5 | 10 | 12 | 8 | 11 | 15 | 16 |
Nucleus | 83 | 40 | 43 | 75 | 74 | 90 | 74 | – | 38 | 23 | 13 | 11 | 0 | 1 | 0 | 131 | 149 | 119 | 5 | 3 | 1 | 1 | 1 | 2 |
Nuclear Periphery | 3 | 0 | 1 | 1 | 0 | 3 | 1 | – | 1 | 2 | 1 | 4 | 1 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 0 | 1 | 3 |
Nucleolus | 9 | 1 | 1 | 1 | 0 | 1 | 0 | – | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 3 | 2 | 1 | 0 | 0 | 1 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 5 | 6 | 6 | 1 | 2 | 1 | 1 | – | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 4 | 1 | 2 | 2 | 4 | 1 | 4 | 9 | 7 |
Vac/Vac Membrane | 276 | 249 | 150 | 217 | 226 | 162 | 231 | – | 222 | 181 | 160 | 127 | 13 | 3 | 5 | 161 | 212 | 159 | 395 | 366 | 280 | 179 | 325 | 266 |
Unique Cell Count | 440 | 400 | 290 | 338 | 329 | 305 | 403 | 523 | 506 | 649 | 538 | 323 | 587 | 615 | 325 | 367 | 296 | 488 | 476 | 330 | 276 | 553 | 566 | |
Labelled Cell Count | 540 | 484 | 360 | 439 | 422 | 405 | 532 | 653 | 626 | 778 | 632 | 329 | 590 | 618 | 397 | 452 | 370 | 488 | 476 | 330 | 276 | 553 | 566 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.6 | 5.3 | 6.2 | 6.1 | 7.2 | 5.0 | 6.4 | – | 5.2 | 5.3 | 4.9 | 4.9 | 4.9 | 4.9 | 5.0 | 7.6 | 8.1 | 7.2 | 4.9 | 4.7 | 4.7 |
Std Deviation (1e-4) | 1.3 | 1.2 | 1.6 | 1.3 | 1.2 | 1.4 | 1.7 | – | 1.4 | 1.4 | 1.2 | 1.2 | 1.6 | 2.0 | 1.7 | 1.8 | 1.5 | 1.8 | 1.7 | 1.6 | 1.7 |
Intensity Change (Log2) | – | – | – | -0.03 | 0.22 | -0.32 | 0.04 | – | -0.25 | -0.23 | -0.33 | -0.33 | -0.33 | -0.33 | -0.31 | 0.3 | 0.39 | 0.22 | -0.32 | -0.4 | -0.38 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -1.9 | -2.6 | -4.3 | 2.3 | – | 6.6 | 9.6 | 13.1 | 12.1 | 14.4 | 19.5 | 20.1 | -6.4 | -8.3 | -6.3 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.7 | 2.7 | 3.6 |
Endosome | -2.2 | -3.4 | -3.7 | -4.5 | – | -5.5 | -5.4 | -5.5 | -6.2 | -4.4 | -6.2 | -6.3 | -1.7 | -2.3 | -3.4 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0.7 | 0 | 6.6 | 1.0 | – | 3.2 | 2.5 | 3.1 | 3.1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 2.4 | 2.4 | 4.3 | 1.2 | – | -3.4 | -5.1 | -7.7 | -7.1 | -7.2 | -9.4 | -9.8 | 7.0 | 7.2 | 6.9 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 3.2 | 4.3 | 0.3 | 1.5 | – | -2.5 | -4.4 | -8.1 | -8.2 | -13.3 | -18.8 | -19.0 | -0.5 | 1.5 | 0.5 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.1416 | 0.4515 | 0.6671 | 0.8963 | 0.5185 | 0.8508 | 2.0325 | 2.1335 | 1.81 | 2.2261 | 2.3879 | 2.9271 | 3.1018 | 4.6529 | 4.1418 | 4.5607 | 5.3465 | 4.869 |
Actin | 0.0227 | 0 | 0.0074 | 0.0238 | 0 | 0 | 0.0494 | 0.0004 | 0.0037 | 0.0381 | 0.0003 | 0.0088 | 0.0001 | 0 | 0.0002 | 0 | 0.0003 | 0.0004 |
Bud | 0.0005 | 0.0007 | 0.0025 | 0.0003 | 0.0002 | 0.0007 | 0.0008 | 0.0006 | 0.0032 | 0.0002 | 0.007 | 0.001 | 0.0003 | 0.0037 | 0.0042 | 0.0021 | 0.0008 | 0.0003 |
Bud Neck | 0.0052 | 0.0011 | 0.0011 | 0.0006 | 0.0012 | 0.0013 | 0.0103 | 0.002 | 0.0026 | 0.0005 | 0.001 | 0.0067 | 0.0008 | 0.0012 | 0.001 | 0.002 | 0.002 | 0.0027 |
Bud Periphery | 0.0008 | 0.0022 | 0.0111 | 0.0005 | 0.0007 | 0.002 | 0.0017 | 0.0014 | 0.0047 | 0.0004 | 0.0022 | 0.0033 | 0.0011 | 0.0069 | 0.0138 | 0.0081 | 0.0022 | 0.001 |
Bud Site | 0.0027 | 0.0021 | 0.0055 | 0.0007 | 0.0006 | 0.0006 | 0.0062 | 0.0023 | 0.0031 | 0.0005 | 0.0014 | 0.0014 | 0.0008 | 0.0033 | 0.0034 | 0.0017 | 0.0016 | 0.0009 |
Cell Periphery | 0.0009 | 0.0023 | 0.0011 | 0.0006 | 0.0009 | 0.002 | 0.0025 | 0.0032 | 0.0015 | 0.0008 | 0.0018 | 0.0028 | 0.0015 | 0.0014 | 0.0022 | 0.0024 | 0.0042 | 0.0025 |
Cytoplasm | 0.0326 | 0.0218 | 0.0188 | 0.0028 | 0.0026 | 0.0077 | 0.0125 | 0.0477 | 0.06 | 0.0174 | 0.0466 | 0.0178 | 0.0115 | 0.0101 | 0.0214 | 0.0033 | 0.0384 | 0.0089 |
Cytoplasmic Foci | 0.0278 | 0.0191 | 0.0332 | 0.0094 | 0.0149 | 0.0157 | 0.0188 | 0.0205 | 0.0336 | 0.0154 | 0.0245 | 0.0372 | 0.0278 | 0.0176 | 0.0168 | 0.0502 | 0.0162 | 0.0192 |
Eisosomes | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0.0009 | 0 | 0.0001 | 0.0002 | 0 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0.0001 |
Endoplasmic Reticulum | 0.0076 | 0.0044 | 0.0034 | 0.0013 | 0.0021 | 0.0028 | 0.0083 | 0.0078 | 0.0077 | 0.0015 | 0.002 | 0.0023 | 0.0031 | 0.0016 | 0.0114 | 0.0057 | 0.0022 | 0.0027 |
Endosome | 0.3567 | 0.2003 | 0.3336 | 0.2185 | 0.3831 | 0.27 | 0.3306 | 0.2179 | 0.3009 | 0.3367 | 0.4442 | 0.2626 | 0.3592 | 0.2469 | 0.3153 | 0.2499 | 0.2885 | 0.2841 |
Golgi | 0.0163 | 0.0049 | 0.0146 | 0.0177 | 0.0065 | 0.0073 | 0.0135 | 0.0058 | 0.0127 | 0.003 | 0.0224 | 0.0129 | 0.0086 | 0.0067 | 0.0068 | 0.0075 | 0.0155 | 0.0107 |
Lipid Particles | 0.0467 | 0.0222 | 0.0194 | 0.0105 | 0.0076 | 0.0436 | 0.0329 | 0.0183 | 0.0135 | 0.0037 | 0.0373 | 0.0525 | 0.0371 | 0.0289 | 0.0198 | 0.0114 | 0.0049 | 0.0588 |
Mitochondria | 0.0296 | 0.0227 | 0.0339 | 0.0051 | 0.0166 | 0.0219 | 0.0164 | 0.0038 | 0.0149 | 0.0013 | 0.0055 | 0.0095 | 0.0066 | 0.0199 | 0.0092 | 0.0028 | 0.0028 | 0.0189 |
None | 0.0514 | 0.0393 | 0.0235 | 0.0002 | 0.0003 | 0.002 | 0.0414 | 0.0446 | 0.0685 | 0.0199 | 0.0105 | 0.0055 | 0.0352 | 0.0168 | 0.0388 | 0.0002 | 0.0019 | 0.0074 |
Nuclear Periphery | 0.0182 | 0.0076 | 0.007 | 0.002 | 0.0064 | 0.0076 | 0.0115 | 0.0048 | 0.0202 | 0.0022 | 0.0022 | 0.0044 | 0.0037 | 0.002 | 0.0047 | 0.0065 | 0.0017 | 0.0031 |
Nucleolus | 0.0033 | 0.0047 | 0.0025 | 0.001 | 0.0017 | 0.0019 | 0.0093 | 0.0039 | 0.0038 | 0.001 | 0.0097 | 0.0033 | 0.0047 | 0.0033 | 0.0023 | 0.0022 | 0.0119 | 0.0054 |
Nucleus | 0.0126 | 0.006 | 0.0071 | 0.0013 | 0.0016 | 0.0035 | 0.0069 | 0.0038 | 0.0087 | 0.0014 | 0.005 | 0.0035 | 0.0036 | 0.0027 | 0.004 | 0.0021 | 0.0028 | 0.0048 |
Peroxisomes | 0.0055 | 0.0016 | 0.0027 | 0.0023 | 0.0005 | 0.0009 | 0.0035 | 0.0015 | 0.003 | 0.0012 | 0.0013 | 0.0078 | 0.0019 | 0.0031 | 0.0013 | 0.0043 | 0.0008 | 0.0041 |
Punctate Nuclear | 0.006 | 0.0017 | 0.0033 | 0.0005 | 0.0001 | 0.0003 | 0.0065 | 0.0005 | 0.0012 | 0.0002 | 0.0024 | 0.0022 | 0.0008 | 0.0004 | 0.0006 | 0.0001 | 0.0001 | 0.0008 |
Vacuole | 0.2895 | 0.5115 | 0.3525 | 0.582 | 0.4505 | 0.5014 | 0.3457 | 0.5186 | 0.3432 | 0.483 | 0.3131 | 0.461 | 0.4035 | 0.5245 | 0.4461 | 0.5531 | 0.5269 | 0.4762 |
Vacuole Periphery | 0.0632 | 0.124 | 0.1155 | 0.1186 | 0.1019 | 0.1069 | 0.0702 | 0.0908 | 0.0892 | 0.0716 | 0.0597 | 0.0935 | 0.088 | 0.0989 | 0.0765 | 0.0843 | 0.0744 | 0.087 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 15.2503 | 33.6811 | 55.9364 | 38.4435 | 9.3048 | 7.1726 | 71.3533 | 88.1084 | 46.8489 | 18.8865 |
Translational Efficiency | 1.0578 | 1.5082 | 0.9691 | 0.7691 | 1.3274 | 1.5467 | 0.7597 | 0.6576 | 0.8196 | 0.7081 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1564 | 1222 | 2177 | 2085 | 1387 | 2411 | 2055 | 91 | 2951 | 3633 | 4232 | 2176 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 742.74 | 715.02 | 777.59 | 837.05 | 695.60 | 795.16 | 787.83 | 959.17 | 720.58 | 768.20 | 782.56 | 842.16 |
Standard Deviation | 94.38 | 113.82 | 93.08 | 116.17 | 84.71 | 100.84 | 94.98 | 119.98 | 92.99 | 111.98 | 94.15 | 118.87 |
Intensity Change Log 2 | — | -0.054874 | 0.066152 | 0.172457 | — | 0.192987 | 0.179626 | 0.463529 | — | 0.070316 | 0.122145 | 0.320559 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000020 | 0.000340 | 0.001084 | 0.030007 | 0.000065 | 0.000145 | 0.004226 | 0.028096 | 0.000042 | 0.000211 | 0.002610 | 0.029927 |
Bud Neck | 0.017554 | 0.032269 | 0.057078 | 0.059631 | 0.024934 | 0.032484 | 0.071825 | 0.043102 | 0.021023 | 0.032412 | 0.064239 | 0.058940 |
Bud Site | 0.015792 | 0.040999 | 0.041848 | 0.156162* | 0.013838 | 0.029902 | 0.065471 | 0.149303* | 0.014874 | 0.033634 | 0.053319 | 0.155875* |
Cell Periphery | 0.000675 | 0.000597 | 0.000432 | 0.005101 | 0.000350 | 0.000709 | 0.000601 | 0.039053 | 0.000522 | 0.000671 | 0.000514 | 0.006521 |
Cytoplasm | 0.036413 | 0.107633* | 0.295033* | 0.192297* | 0.069106 | 0.058545 | 0.269487* | 0.122034* | 0.051779 | 0.075056 | 0.282628* | 0.189358* |
Cytoplasmic Foci | 0.016123 | 0.058469 | 0.048948 | 0.077220 | 0.028161 | 0.025197 | 0.079161 | 0.096645 | 0.021781 | 0.036389 | 0.063619 | 0.078033 |
Eisosomes | 0.000020 | 0.000045 | 0.000090 | 0.000515 | 0.000029 | 0.000031 | 0.000190 | 0.002522 | 0.000024 | 0.000036 | 0.000138 | 0.000599 |
Endoplasmic Reticulum | 0.001510 | 0.001918 | 0.006766 | 0.004169 | 0.001896 | 0.003108 | 0.003490 | 0.004980 | 0.001691 | 0.002708 | 0.005175 | 0.004203 |
Endosome | 0.101061 | 0.123707 | 0.052590 | 0.123813 | 0.100927 | 0.117175 | 0.101408 | 0.075418 | 0.100998 | 0.119372 | 0.076295 | 0.121790 |
Golgi | 0.001600 | 0.004543 | 0.000943 | 0.008486 | 0.001020 | 0.003221 | 0.001659 | 0.002100 | 0.001328 | 0.003666 | 0.001291 | 0.008219 |
Lipid Particles | 0.000960 | 0.003143 | 0.009271 | 0.012020 | 0.001952 | 0.001771 | 0.010209 | 0.008581 | 0.001426 | 0.002232 | 0.009727 | 0.011876 |
Mitochondria | 0.001437 | 0.003707 | 0.002330 | 0.002859 | 0.003184 | 0.005201 | 0.002215 | 0.000183 | 0.002258 | 0.004699 | 0.002274 | 0.002747 |
Mitotic Spindle | 0.000136 | 0.000823 | 0.017762 | 0.012161 | 0.000277 | 0.000829 | 0.013111 | 0.004780 | 0.000202 | 0.000827 | 0.015503 | 0.011852 |
None | 0.000821 | 0.002100 | 0.003689 | 0.000644 | 0.000663 | 0.001050 | 0.000803 | 0.000114 | 0.000747 | 0.001403 | 0.002287 | 0.000622 |
Nuclear Periphery | 0.000137 | 0.000270 | 0.002447 | 0.000289 | 0.000300 | 0.000352 | 0.000895 | 0.000394 | 0.000214 | 0.000325 | 0.001694 | 0.000293 |
Nuclear Periphery Foci | 0.000126 | 0.000450 | 0.003809 | 0.000853 | 0.000144 | 0.000474 | 0.001325 | 0.004468 | 0.000134 | 0.000466 | 0.002603 | 0.001004 |
Nucleolus | 0.000489 | 0.001865 | 0.002832 | 0.000561 | 0.001019 | 0.001707 | 0.001468 | 0.000236 | 0.000738 | 0.001760 | 0.002170 | 0.000547 |
Nucleus | 0.007483 | 0.023586 | 0.317845* | 0.028219 | 0.013697 | 0.010687 | 0.093548 | 0.025598 | 0.010404 | 0.015026 | 0.208930* | 0.028109 |
Peroxisomes | 0.001227 | 0.004688 | 0.011061 | 0.042133 | 0.001440 | 0.002464 | 0.029030 | 0.037197 | 0.001327 | 0.003212 | 0.019786 | 0.041927 |
Vacuole | 0.768751* | 0.563387* | 0.123478 | 0.240823* | 0.718115* | 0.682399* | 0.248745* | 0.354969* | 0.744952* | 0.642368* | 0.184306 | 0.245597* |
Vacuole Periphery | 0.027662 | 0.025461 | 0.000665 | 0.002038 | 0.018883 | 0.022549 | 0.001131 | 0.000224 | 0.023535 | 0.023528 | 0.000892 | 0.001962 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -1.80 | -8.31 | -11.98 | -11.76 | -11.50 | -1.75 | -7.37 | -2.97 | -2.96 | -2.42 | -2.61 | -9.16 | -12.25 | -12.16 | -11.08 |
Bud Neck | -5.28 | -11.08 | -12.65 | -6.00 | 0.45 | -2.78 | -10.94 | -0.51 | 0.29 | 3.36 | -5.98 | -15.64 | -11.88 | -7.01 | 2.40 |
Bud Site | -7.76 | -6.52 | -23.39 | -16.06 | -18.44 | -7.64 | -12.84 | -4.09 | -3.36 | -2.34 | -10.57 | -13.74 | -24.46 | -19.73 | -17.43 |
Cell Periphery | 0.68 | 3.59 | -3.73 | -3.81 | -4.13 | -3.90 | -1.32 | -1.93 | -1.90 | -1.92 | -1.88 | 1.86 | -4.32 | -4.16 | -4.44 |
Cytoplasm | -11.35 | -37.30 | -29.15 | -13.24 | 8.78 | 2.24 | -28.43 | -2.80 | -3.39 | 7.01 | -6.14 | -46.40 | -27.40 | -22.99 | 10.92 |
Cytoplasmic Foci | -12.14 | -11.70 | -18.12 | -2.25 | -7.08 | 1.24 | -12.93 | -4.14 | -4.39 | -0.92 | -7.55 | -17.42 | -17.14 | -11.23 | -3.50 |
Eisosomes | -6.36 | -13.96 | -6.83 | -6.37 | -5.69 | -0.79 | -5.93 | -2.19 | -2.18 | -2.01 | -5.26 | -8.88 | -6.96 | -6.78 | -5.38 |
Endoplasmic Reticulum | -2.07 | -8.36 | -5.65 | -4.61 | 3.32 | -4.65 | -4.43 | -3.14 | -2.00 | -1.76 | -5.69 | -9.45 | -5.60 | -3.19 | 1.54 |
Endosome | -3.13 | 10.60 | 0.59 | 3.87 | -10.88 | -2.64 | 3.20 | 3.05 | 4.30 | 1.70 | -3.95 | 9.55 | 1.00 | 4.59 | -7.23 |
Golgi | -4.78 | 4.53 | -7.93 | -2.20 | -9.49 | -6.09 | -1.47 | -0.97 | 3.26 | -0.41 | -7.33 | 2.71 | -8.54 | -3.92 | -9.17 |
Lipid Particles | -4.66 | -10.12 | -9.56 | -6.89 | -1.63 | 0.36 | -8.85 | -3.40 | -3.53 | 0.42 | -2.66 | -13.42 | -9.34 | -8.53 | -1.27 |
Mitochondria | -2.49 | -2.67 | -3.25 | 1.34 | -0.78 | -2.01 | 1.15 | 3.16 | 9.48 | 7.40 | -3.94 | 0.11 | -0.32 | 4.72 | -0.83 |
Mitotic Spindle | -2.70 | -9.58 | -8.19 | -7.57 | 2.39 | -3.54 | -7.99 | -2.21 | -2.12 | 0.17 | -4.82 | -12.44 | -8.42 | -7.94 | 2.24 |
None | -1.80 | -4.37 | 0.49 | 2.14 | 4.86 | -0.75 | -0.69 | 0.54 | 1.44 | 2.52 | -1.61 | -4.13 | 0.37 | 1.85 | 4.26 |
Nuclear Periphery | -1.88 | -19.34 | -7.72 | -3.67 | 16.57 | -0.28 | -6.79 | -2.59 | -2.42 | 0.33 | -1.00 | -19.03 | -5.05 | -2.57 | 16.68 |
Nuclear Periphery Foci | -4.17 | -6.17 | -6.73 | -3.69 | 4.93 | -1.55 | -8.91 | -3.43 | -3.28 | -2.75 | -2.36 | -7.87 | -7.29 | -4.07 | 4.59 |
Nucleolus | -5.01 | -8.19 | -1.37 | 4.10 | 7.63 | -1.98 | -3.25 | 2.03 | 4.56 | 6.24 | -4.59 | -8.63 | 0.25 | 4.87 | 8.87 |
Nucleus | -6.07 | -48.93 | -14.97 | -5.30 | 42.46 | 1.81 | -24.22 | -3.10 | -3.40 | 5.30 | -3.44 | -49.91 | -14.25 | -11.69 | 39.45 |
Peroxisomes | -4.99 | -9.52 | -15.90 | -13.57 | -11.26 | -3.86 | -13.38 | -3.45 | -3.30 | -0.28 | -6.36 | -16.18 | -16.23 | -15.18 | -8.07 |
Vacuole | 17.06 | 75.08* | 48.50* | 22.46 | -21.28 | 3.28 | 43.81* | 7.73 | 6.85 | -4.39 | 13.15 | 81.55* | 52.49* | 40.28* | -13.82 |
Vacuole Periphery | 1.24 | 19.56 | 18.44 | 17.07 | -3.74 | -2.52 | 15.29 | 16.24 | 23.82 | 2.61 | 0.04 | 24.71 | 23.07 | 26.84 | -2.81 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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