Standard name
Human Ortholog
Description 3-ketoacyl-CoA thiolase with broad chain length specificity; cleaves 3-ketoacyl-CoA into acyl-CoA and acetyl-CoA during beta-oxidation of fatty acids
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0.06 0 0.07 0.07 0.06 0.08
Bud 0 0 0 0 0 0 0.05 0.07 0.13 0.13 0.16 0.13 0 0 0 0 – 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0.08 0.06 0 0 0 0 – 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Cytoplasm 0.91 0.9 0.88 0.9 0.85 0.73 0.69 0.64 0.46 0.42 0.26 0.28 0.85 0.87 0.87 0.9 – 0.9 0.57 0.65 0.37 0.43 0.42 0.46
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0.1 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0.05 0.06 0.08 0.05 0.05
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Mitochondria 0.12 0.06 0 0 0.12 0.31 0.34 0.32 0.39 0.49 0.53 0.53 0 0 0 0 – 0 0.14 0.07 0.25 0.08 0.09 0.13
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0.1 0.1 0.18 0.2 0 0 0 0 – 0 0 0 0 0.05 0.07 0.06
SpindlePole 0 0 0.1 0.06 0.08 0.07 0.1 0.15 0.18 0.17 0.22 0.22 0.11 0.11 0.1 0 – 0 0.06 0.08 0.09 0.18 0.15 0.09
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 5 3 0 0 0 0 0 3 6 6 2 5 4 0 3 0 – 0 25 6 45 23 20 32
Bud 8 5 6 8 11 23 34 43 71 80 90 77 4 0 4 0 – 0 5 4 12 11 14 9
Bud Neck 8 1 12 4 3 10 13 13 13 15 8 6 8 10 15 9 – 8 9 6 14 13 13 15
Bud Site 0 4 2 0 1 7 4 6 9 26 44 35 3 1 3 1 – 0 – – – – – –
Cell Periphery 4 2 9 9 2 2 7 1 4 1 5 1 0 1 3 9 – 3 8 1 11 2 3 4
Cytoplasm 387 222 535 362 364 410 480 405 261 261 150 163 429 432 544 363 – 241 244 170 227 153 143 187
Endoplasmic Reticulum 0 0 0 0 0 1 0 0 2 0 0 0 0 1 1 18 – 27 3 0 7 1 2 1
Endosome 0 3 6 5 5 4 6 5 9 8 21 16 5 9 8 9 – 6 15 12 33 27 16 19
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 1 1 1 4 2 4 4
Mitochondria 50 14 4 15 51 173 238 204 219 306 303 302 2 1 1 6 – 12 59 19 153 27 29 55
Nucleus 0 0 1 0 0 1 5 2 0 2 2 4 2 0 2 3 – 2 0 0 0 0 2 1
Nuclear Periphery 0 1 0 2 0 0 0 0 1 0 0 0 0 0 0 0 – 0 1 0 1 0 0 1
Nucleolus 0 2 0 0 0 0 0 1 2 3 2 5 0 0 0 0 – 0 0 0 1 0 0 0
Peroxisomes 1 4 10 6 2 16 12 23 56 62 100 116 6 4 5 3 – 0 8 2 16 17 25 23
SpindlePole 12 7 64 26 35 40 68 96 103 105 128 128 57 53 64 18 – 6 24 21 57 62 51 34
Vac/Vac Membrane 0 0 4 3 2 2 4 5 5 0 9 2 1 0 1 2 – 4 6 6 6 6 7 8
Unique Cell Count 425 247 611 402 426 558 692 632 563 624 570 575 504 497 624 403 267 431 260 616 357 342 408
Labelled Cell Count 475 268 653 440 476 689 871 807 761 875 864 860 521 512 654 441 310 431 260 616 357 342 408


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 2.9 3.7 4.3 4.1 3.8 3.3 3.2 3.4 3.3 3.1 2.9 3.2 5.2 5.1 4.9 5.3 4.9 5.2 5.2 5.6 5.8
Std Deviation (1e-4) 0.5 0.7 1.6 2.5 1.8 1.5 1.3 1.9 1.6 1.2 1.0 1.7 1.0 1.1 1.1 1.9 1.7 1.9 1.5 1.8 2.0
Intensity Change (Log2) – – – -0.07 -0.17 -0.39 -0.4 -0.33 -0.36 -0.45 -0.55 -0.42 0.28 0.24 0.18 0.3 0.19 0.27 0.29 0.38 0.44


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.7046 1.4443 1.3433 0.4234 1.0571 1.3188 0.9351 0.2308 0.4526 0.4408 -0.3577 0.353 -0.3907 -0.3215 -0.1807 -0.2084 0.6542 -0.1992
Actin 0.0642 0.0112 0.0133 0.0013 0.0029 0.0064 0.0823 0.002 0.0171 0.0194 0.0215 0.0101 0.0382 0.0045 0.0098 0.0042 0.022 0.0173
Bud 0.0016 0.0087 0.0019 0.0005 0.0016 0.0002 0.0038 0.0122 0.001 0.0005 0.0006 0.0016 0.0164 0.0361 0.0252 0.0141 0.0312 0.0206
Bud Neck 0.0053 0.0032 0.0272 0.0003 0.0084 0.0126 0.0113 0.0035 0.0483 0.0598 0.1381 0.0146 0.004 0.0039 0.0197 0.0021 0.002 0.0209
Bud Periphery 0.0031 0.0091 0.0019 0.0016 0.0048 0.0005 0.0136 0.0139 0.0026 0.0008 0.0011 0.0034 0.0136 0.0127 0.0122 0.0106 0.0159 0.0139
Bud Site 0.0098 0.0578 0.0207 0.0001 0.0031 0.0013 0.0175 0.0655 0.0098 0.0028 0.0021 0.0005 0.0287 0.1168 0.0426 0.0148 0.0056 0.004
Cell Periphery 0.0009 0.0008 0.0006 0 0.0006 0.0001 0.0004 0.0007 0.0004 0.0001 0.0001 0.0002 0.0017 0.0017 0.0012 0.0003 0.001 0.0007
Cytoplasm 0.0186 0.0466 0.0229 0.0626 0.0136 0.033 0.0178 0.0787 0.0272 0.0507 0.0404 0.0571 0.0394 0.0485 0.0479 0.0253 0.031 0.0685
Cytoplasmic Foci 0.2934 0.1919 0.2415 0.2769 0.26 0.3412 0.1716 0.223 0.2207 0.1648 0.3338 0.2433 0.2637 0.1805 0.1649 0.3203 0.1491 0.2153
Eisosomes 0.0012 0.0004 0.0008 0.0002 0.0001 0.0001 0.0005 0.0002 0.0003 0.0002 0.0003 0.0001 0.0006 0.0003 0.0005 0.0002 0.0009 0.0002
Endoplasmic Reticulum 0.0026 0.0013 0.0029 0.0008 0.0038 0.0002 0.003 0.0012 0.001 0.0019 0.0005 0.0009 0.0017 0.0006 0.003 0.0009 0.0019 0.0012
Endosome 0.0227 0.0108 0.0061 0.0134 0.0341 0.0063 0.015 0.014 0.0149 0.0337 0.0043 0.0154 0.0164 0.0071 0.0128 0.0084 0.0062 0.0064
Golgi 0.0095 0.0034 0.0029 0.0031 0.0032 0.0032 0.0069 0.0028 0.006 0.0114 0.0079 0.0038 0.0066 0.0021 0.0032 0.0026 0.0041 0.0019
Lipid Particles 0.0251 0.0062 0.0084 0.0084 0.0168 0.0158 0.0173 0.0093 0.0244 0.0309 0.0093 0.0164 0.0212 0.0055 0.0102 0.0124 0.0104 0.0165
Mitochondria 0.0109 0.0059 0.0059 0.0056 0.0029 0.0077 0.0049 0.0034 0.0131 0.005 0.0156 0.0041 0.0029 0.0019 0.003 0.007 0.004 0.0025
None 0.409 0.4382 0.4286 0.3846 0.3767 0.4011 0.5121 0.467 0.4484 0.4791 0.355 0.5367 0.4725 0.4571 0.4968 0.3446 0.4345 0.4756
Nuclear Periphery 0.0028 0.0015 0.0046 0.0016 0.005 0.0005 0.0191 0.0006 0.003 0.0015 0.0002 0.0053 0.0071 0.0005 0.0038 0.003 0.0134 0.0031
Nucleolus 0.0025 0.0024 0.004 0.0008 0.0021 0.0007 0.0009 0.0025 0.0024 0.0005 0.0004 0.0006 0.0022 0.0018 0.0031 0.0048 0.0101 0.0014
Nucleus 0.0011 0.0069 0.0032 0.0006 0.0042 0.0007 0.0161 0.0019 0.0017 0.0014 0.0004 0.0016 0.0039 0.0047 0.0108 0.0366 0.0147 0.009
Peroxisomes 0.1004 0.171 0.1613 0.1701 0.176 0.1485 0.0725 0.077 0.1209 0.0894 0.056 0.0631 0.0406 0.0893 0.0832 0.1191 0.0839 0.0846
Punctate Nuclear 0.0135 0.0162 0.0402 0.0654 0.072 0.0193 0.0106 0.0177 0.0265 0.0446 0.0116 0.0175 0.0164 0.0228 0.0437 0.0661 0.1541 0.0341
Vacuole 0.0013 0.0057 0.0007 0.0012 0.007 0.0004 0.0014 0.0026 0.0078 0.0012 0.0005 0.003 0.0016 0.0014 0.0019 0.0018 0.0022 0.0018
Vacuole Periphery 0.0005 0.0007 0.0003 0.0007 0.0012 0.0001 0.0014 0.0004 0.0021 0.0004 0.0003 0.0009 0.0005 0.0002 0.0005 0.0005 0.0016 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression – – – – – – – – – –
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression –
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1812 398 1746 741 1316 1590 112 887 3128 1988 1858 1628

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 640.66 621.23 749.08 730.01 640.89 676.02 850.32 796.30 640.76 665.05 755.18 766.13
Standard Deviation 71.76 110.35 91.82 114.15 72.89 81.19 102.92 125.03 72.24 90.51 95.61 124.65
Intensity Change Log 2 — -0.044431 0.225561 0.188357 — 0.076989 0.407929 0.313235 — 0.017567 0.319641 0.252158

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000297 0.002196 0.002534 0.024972 0.000188 0.001600 0.003585 0.012233 0.000251 0.001719 0.002597 0.018031
Bud Neck 0.022819 0.043093 0.016531 0.008957 0.019108 0.049485 0.002061 0.014246 0.021258 0.048205 0.015659 0.011839
Bud Site 0.005023 0.032513 0.034946 0.049207 0.006896 0.030937 0.007664 0.064415 0.005811 0.031252 0.033301 0.057493
Cell Periphery 0.000187 0.000209 0.000268 0.000511 0.000197 0.000271 0.000308 0.000294 0.000191 0.000259 0.000271 0.000393
Cytoplasm 0.385686* 0.226507* 0.332817* 0.327521* 0.474538* 0.296722* 0.183988* 0.334595* 0.423068* 0.282665* 0.323846* 0.331375*
Cytoplasmic Foci 0.244337* 0.331398* 0.073554 0.090295 0.265248* 0.297761* 0.061195 0.085388 0.253134* 0.304495* 0.072809 0.087622
Eisosomes 0.000231 0.000261 0.000080 0.000226 0.000232 0.000222 0.000201 0.000145 0.000231 0.000230 0.000088 0.000182
Endoplasmic Reticulum 0.000870 0.000512 0.001550 0.004613 0.000400 0.000446 0.003294 0.003916 0.000672 0.000459 0.001655 0.004233
Endosome 0.011055 0.041856 0.008755 0.007232 0.008311 0.032823 0.009695 0.024147 0.009901 0.034631 0.008811 0.016448
Golgi 0.004744 0.012102 0.001298 0.001845 0.002428 0.012333 0.004751 0.007590 0.003770 0.012287 0.001506 0.004975
Lipid Particles 0.012946 0.017716 0.004238 0.004552 0.009601 0.010285 0.005803 0.007033 0.011538 0.011772 0.004332 0.005904
Mitochondria 0.009835 0.008145 0.001812 0.006351 0.004365 0.018519 0.000966 0.007139 0.007534 0.016442 0.001761 0.006780
Mitotic Spindle 0.000330 0.009943 0.018141 0.011103 0.000224 0.009261 0.000265 0.052671 0.000286 0.009398 0.017063 0.033751
None 0.006009 0.012628 0.006181 0.006786 0.004318 0.002477 0.004049 0.003599 0.005298 0.004510 0.006053 0.005049
Nuclear Periphery 0.000482 0.000365 0.001465 0.001164 0.000356 0.000361 0.001396 0.001078 0.000429 0.000362 0.001460 0.001117
Nuclear Periphery Foci 0.000196 0.002527 0.001132 0.003066 0.000273 0.000338 0.001684 0.002220 0.000229 0.000776 0.001165 0.002605
Nucleolus 0.001679 0.005107 0.002041 0.000811 0.001098 0.001886 0.001284 0.000817 0.001435 0.002531 0.001995 0.000814
Nucleus 0.249569* 0.067996 0.398928* 0.252959* 0.160803* 0.097546 0.592054* 0.170698* 0.212224* 0.091630 0.410569* 0.208140*
Peroxisomes 0.015376 0.147745* 0.049631 0.073176 0.018327 0.084435 0.027687 0.074993 0.016617 0.097110 0.048308 0.074166
Vacuole 0.027062 0.035776 0.043753 0.124034 0.022349 0.050030 0.087755 0.128570 0.025079 0.047176 0.046405 0.126505
Vacuole Periphery 0.001266 0.001406 0.000346 0.000619 0.000740 0.002262 0.000313 0.004212 0.001044 0.002091 0.000344 0.002577

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.16 -3.68 -5.30 -4.47 -4.55 -2.97 -2.09 -4.04 -3.18 -2.17 -3.48 -4.06 -6.65 -5.59 -5.20
Bud Neck -3.95 2.67 7.72 7.20 5.03 -8.82 7.34 3.19 12.56 -5.14 -9.94 2.68 7.32 15.12 4.03
Bud Site -6.86 -10.73 -7.72 -1.06 -1.03 -9.18 -0.20 -7.76 -2.43 -6.39 -12.02 -10.34 -10.99 -2.62 -2.42
Cell Periphery -0.69 -4.23 -5.35 -4.49 -3.58 -1.72 -2.61 -1.25 0.92 1.83 -1.93 -4.66 -5.19 -1.45 -1.95
Cytoplasm 10.83 8.66 7.62 -4.27 0.65 17.35 15.00 13.61 -1.11 -6.44 17.66 15.34 14.14 -2.30 -0.75
Cytoplasmic Foci -7.19 24.85 23.32 20.92 1.00 -3.63 16.01 24.97 30.38 -1.44 -7.74 32.14 34.08 37.31 0.73
Eisosomes -0.52 8.86 0.56 0.79 -6.69 0.38 -0.19 3.08 3.27 2.86 0.20 8.72 3.10 2.33 -6.71
Endoplasmic Reticulum 2.50 -7.80 -13.02 -14.13 -8.56 -0.84 -6.47 -3.22 -3.16 1.18 2.57 -10.67 -7.35 -7.77 -3.59
Endosome -7.33 1.41 5.24 8.66 3.05 -12.37 0.06 -3.63 7.10 -2.15 -14.10 0.65 -0.92 12.32 -1.31
Golgi -5.31 7.50 5.85 8.08 0.04 -9.13 -0.07 -1.19 6.02 -0.40 -9.16 7.24 2.07 9.37 -2.13
Lipid Particles -1.64 8.37 8.25 4.13 -0.09 -0.80 2.01 3.46 4.46 1.01 -0.46 8.86 8.05 6.73 -0.44
Mitochondria 1.04 7.39 2.97 2.06 -1.02 -7.51 3.51 0.43 7.17 -2.22 -5.83 7.56 2.97 7.49 -1.78
Mitotic Spindle -4.23 -9.23 -4.76 -0.27 2.79 -6.66 -1.05 -8.06 -5.98 -7.14 -7.75 -9.28 -9.15 -5.52 -2.36
None -1.97 0.74 0.95 2.25 0.53 2.73 -0.57 1.92 -0.79 1.63 0.68 -0.06 1.97 0.93 1.89
Nuclear Periphery 1.25 -17.83 -13.07 -11.41 2.20 -0.39 -9.23 -10.98 -11.07 0.85 1.31 -20.23 -16.09 -16.61 2.28
Nuclear Periphery Foci -2.51 -6.17 -7.17 -0.35 -2.61 -1.46 -2.83 -4.94 -4.69 0.37 -2.90 -6.49 -8.46 -5.10 -1.92
Nucleolus -3.57 -3.08 5.45 4.31 4.51 -2.93 -1.24 0.72 3.48 1.54 -4.24 -3.42 3.69 6.02 4.32
Nucleus 18.23 -14.90 -1.68 -16.97 11.18 10.07 -13.70 -4.27 -11.33 12.04 20.76 -22.66 -2.55 -19.51 18.59
Peroxisomes -11.77 -7.06 -4.98 8.30 -0.27 -13.77 -0.20 -4.77 5.97 -2.16 -18.64 -6.80 -7.02 9.44 -0.73
Vacuole -2.16 -12.10 -21.07 -18.90 -16.40 -10.50 -6.38 -23.13 -19.37 -4.82 -10.19 -14.50 -31.16 -26.40 -22.56
Vacuole Periphery -0.32 5.58 4.66 3.65 -1.00 -3.52 2.25 -2.13 0.27 -3.02 -3.05 5.59 -0.73 1.72 -3.07
Standard name
Human Ortholog
Description 3-ketoacyl-CoA thiolase with broad chain length specificity; cleaves 3-ketoacyl-CoA into acyl-CoA and acetyl-CoA during beta-oxidation of fatty acids
Localization
Cell Percentages cytoplasm (15%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Pot1

Pot1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pot1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available