Standard name | |
---|---|
Human Ortholog | |
Description | Protein of unknown function; null mutant displays sensitivity to DNA damaging agents; may have a role in lipid metabolism, based on localization to lipid droplets; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 6 | 8 | 8 | 9 | 13 | 9 | 7 | 19 | 13 | 14 | 13 | 18 | 9 | 13 | – | – | – | 0 | 1 | 6 | 6 | 6 | 9 |
Bud | 0 | 0 | 3 | 2 | 5 | 17 | 30 | 29 | 22 | 20 | 25 | 18 | 2 | 0 | 0 | – | – | – | 0 | 0 | 0 | 2 | 4 | 3 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 1 | 2 | 3 | 3 | 3 | 6 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 4 | 2 | 3 | 0 | 0 | 0 | – | – | – | – | – | – | – | – | – |
Cell Periphery | 0 | 6 | 5 | 10 | 16 | 9 | 13 | 4 | 8 | 9 | 5 | 5 | 21 | 26 | 34 | – | – | – | 0 | 0 | 2 | 2 | 4 | 7 |
Cytoplasm | 6 | 52 | 76 | 83 | 152 | 133 | 168 | 93 | 123 | 95 | 86 | 84 | 126 | 52 | 104 | – | – | – | 3 | 17 | 19 | 27 | 24 | 21 |
Endoplasmic Reticulum | 12 | 100 | 185 | 130 | 161 | 175 | 160 | 114 | 134 | 99 | 99 | 129 | 257 | 196 | 293 | – | – | – | 30 | 136 | 282 | 255 | 256 | 253 |
Endosome | 0 | 0 | 0 | 7 | 7 | 4 | 34 | 32 | 26 | 45 | 10 | 14 | 8 | 3 | 6 | – | – | – | 0 | 2 | 0 | 1 | 3 | 2 |
Golgi | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 5 | 3 | 5 | 1 | 1 | 6 | – | – | – | 0 | 0 | 0 | 2 | 4 | 5 |
Mitochondria | 0 | 0 | 23 | 39 | 67 | 118 | 265 | 248 | 286 | 291 | 370 | 458 | 2 | 0 | 0 | – | – | – | 0 | 0 | 9 | 16 | 11 | 9 |
Nucleus | 0 | 0 | 0 | 1 | 0 | 2 | 8 | 4 | 5 | 9 | 9 | 13 | 0 | 1 | 0 | – | – | – | 0 | 1 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 1 | 1 | 1 | 3 | 7 | 9 | 12 | 10 | 19 | 13 | 0 | 0 | 0 | – | – | – | 0 | 0 | 1 | 0 | 0 | 2 |
Nucleolus | 0 | 0 | 0 | 0 | 1 | 0 | 7 | 11 | 6 | 13 | 8 | 22 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 6 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 6 | 3 | 11 | 45 | 62 | 225 | 188 | 187 | 262 | 121 | 109 | 29 | 8 | 12 | – | – | – | 0 | 6 | 5 | 18 | 23 | 23 |
Unique Cell Count | 16 | 143 | 270 | 241 | 387 | 424 | 736 | 597 | 674 | 721 | 617 | 730 | 397 | 263 | 412 | 38 | 173 | 335 | 340 | 348 | 347 | |||
Labelled Cell Count | 18 | 171 | 305 | 293 | 464 | 536 | 926 | 744 | 829 | 882 | 771 | 886 | 464 | 296 | 468 | 38 | 173 | 335 | 340 | 348 | 347 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 8.9 | 11.6 | 7.3 | 7.4 | 7.3 | 6.9 | 6.7 | 7.1 | 7.4 | 7.3 | 7.1 | 7.3 | 10.1 | 10.8 | 11.7 | – | – | – | 10.8 | 10.9 | 11.4 |
Std Deviation (1e-4) | 1.9 | 1.8 | 1.1 | 1.3 | 1.3 | 1.1 | 1.3 | 1.5 | 1.6 | 1.6 | 1.8 | 1.8 | 1.3 | 1.5 | 1.7 | – | – | – | 2.2 | 2.0 | 2.0 |
Intensity Change (Log2) | – | – | – | 0.02 | -0.0 | -0.09 | -0.12 | -0.05 | 0.02 | 0.0 | -0.03 | -0.01 | 0.48 | 0.56 | 0.68 | – | – | – | 0.57 | 0.57 | 0.65 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0.2 | -0.5 | 0.1 | 0 | 0 | -0.1 | -1.1 | -0.6 | -1.2 | 1.0 | 0.3 | 0.1 | – | – | – |
Bud | 0 | 0 | 2.2 | 2.3 | 2.7 | 1.9 | 1.5 | 2.3 | 1.3 | 0 | 0 | 0 | – | – | – |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Cell Periphery | 1.5 | 1.6 | 0.2 | 0 | 0 | 0 | 0 | 0 | 0 | 2.3 | 4.0 | 3.5 | – | – | – |
Cytoplasm | 1.5 | 2.9 | 0.9 | -1.7 | -4.3 | -3.4 | -5.6 | -5.0 | -6.4 | 1.0 | -2.3 | -0.8 | – | – | – |
Endoplasmic Reticulum | -3.4 | -6.8 | -7.0 | -13.9 | -14.2 | -14.3 | -17.0 | -15.4 | -15.4 | -1.0 | 1.5 | 0.7 | – | – | – |
Endosome | 0 | 0 | 0 | 3.6 | 3.9 | 3.3 | 4.2 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Mitochondria | 2.6 | 3.2 | 6.2 | 8.5 | 9.7 | 10.0 | 9.6 | 14.2 | 15.2 | -5.3 | -4.8 | -6.0 | – | – | – |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.5 | 0 | 0 | 0 | 0 | – | – | – |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.9 | 0 | 0 | 0 | – | – | – |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Vacuole | 2.4 | 5.1 | 6.0 | 9.9 | 10.0 | 9.2 | 11.2 | 7.3 | 6.2 | 3.7 | 1.6 | 1.6 | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 4.511 | 5.506 | 4.5931 | 4.0009 | 3.9821 | 4.875 | 6.2524 | 7.3067 | 6.6958 | 6.3541 | 5.0964 | 6.6202 | 5.465 | 5.8242 | 5.1415 | 5.7688 | 4.8014 | 5.4203 |
Actin | 0.0099 | 0.0013 | 0.0128 | 0.0033 | 0.0126 | 0.0039 | 0.0144 | 0.0103 | 0.0128 | 0.004 | 0.0045 | 0.0039 | 0.0112 | 0.0016 | 0.0068 | 0.0007 | 0.0023 | 0.0008 |
Bud | 0.0073 | 0.0016 | 0.0056 | 0.0002 | 0.0008 | 0.0027 | 0.0002 | 0.0001 | 0.0011 | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0002 | 0 |
Bud Neck | 0.0006 | 0.001 | 0.0019 | 0.0007 | 0.0013 | 0.0016 | 0.0006 | 0.0006 | 0.0051 | 0.0014 | 0.0012 | 0.0012 | 0.0003 | 0.0004 | 0.0006 | 0.0006 | 0.0006 | 0.0009 |
Bud Periphery | 0.0022 | 0.001 | 0.0022 | 0.0006 | 0.0017 | 0.0007 | 0.0002 | 0.0002 | 0.0029 | 0.0012 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0004 | 0.0002 | 0.0001 |
Bud Site | 0.0021 | 0.0195 | 0.0129 | 0.0005 | 0.0019 | 0.0006 | 0.0015 | 0.0022 | 0.0051 | 0.001 | 0.0002 | 0.0002 | 0.0003 | 0.0015 | 0.0005 | 0.0001 | 0.0004 | 0.0001 |
Cell Periphery | 0.0016 | 0.0035 | 0.0031 | 0.0012 | 0.0034 | 0.0015 | 0.0019 | 0.0057 | 0.0054 | 0.003 | 0.0035 | 0.0025 | 0.0013 | 0.0016 | 0.0016 | 0.0022 | 0.0008 | 0.001 |
Cytoplasm | 0.1168 | 0.2307 | 0.2057 | 0.1535 | 0.2038 | 0.2046 | 0.1053 | 0.1614 | 0.1248 | 0.1821 | 0.1908 | 0.1384 | 0.0474 | 0.09 | 0.093 | 0.0382 | 0.221 | 0.0888 |
Cytoplasmic Foci | 0.0446 | 0.0127 | 0.0285 | 0.0148 | 0.0447 | 0.0171 | 0.0284 | 0.0139 | 0.0199 | 0.0194 | 0.0141 | 0.0145 | 0.0256 | 0.02 | 0.0218 | 0.0283 | 0.0274 | 0.014 |
Eisosomes | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0.0003 | 0.0001 | 0.0003 | 0.0018 | 0.0004 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 | 0 | 0 |
Endoplasmic Reticulum | 0.5291 | 0.589 | 0.5244 | 0.6066 | 0.3839 | 0.6384 | 0.6253 | 0.6377 | 0.6419 | 0.654 | 0.6854 | 0.7085 | 0.6352 | 0.6683 | 0.6596 | 0.6783 | 0.5 | 0.6889 |
Endosome | 0.1168 | 0.0492 | 0.0881 | 0.0718 | 0.1154 | 0.0544 | 0.0742 | 0.0414 | 0.0674 | 0.0518 | 0.0305 | 0.0423 | 0.0779 | 0.0479 | 0.059 | 0.0692 | 0.1218 | 0.0748 |
Golgi | 0.033 | 0.008 | 0.0199 | 0.0106 | 0.0329 | 0.0103 | 0.0291 | 0.0099 | 0.0195 | 0.0225 | 0.0079 | 0.0159 | 0.016 | 0.0087 | 0.0153 | 0.0411 | 0.0131 | 0.0135 |
Lipid Particles | 0.0518 | 0.0284 | 0.0418 | 0.0592 | 0.0487 | 0.0276 | 0.0746 | 0.0842 | 0.0542 | 0.0211 | 0.0404 | 0.041 | 0.1473 | 0.1348 | 0.1157 | 0.1139 | 0.0847 | 0.0918 |
Mitochondria | 0.0078 | 0.0013 | 0.0119 | 0.0224 | 0.0412 | 0.0019 | 0.004 | 0.0014 | 0.0115 | 0.006 | 0.0016 | 0.0056 | 0.001 | 0.0004 | 0.0012 | 0.0016 | 0.0056 | 0.0016 |
None | 0.013 | 0.0166 | 0.0113 | 0.0102 | 0.0622 | 0.0128 | 0.0073 | 0.0065 | 0.0013 | 0.0014 | 0.0082 | 0.0091 | 0.0084 | 0.0017 | 0.0011 | 0.001 | 0.0017 | 0.0011 |
Nuclear Periphery | 0.0219 | 0.0135 | 0.0098 | 0.0258 | 0.0117 | 0.0086 | 0.0097 | 0.0082 | 0.007 | 0.009 | 0.005 | 0.0048 | 0.0142 | 0.0118 | 0.0123 | 0.0082 | 0.0072 | 0.0102 |
Nucleolus | 0.0011 | 0.0005 | 0.0004 | 0.0003 | 0.001 | 0.0002 | 0.0003 | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0 |
Nucleus | 0.008 | 0.0055 | 0.0028 | 0.0016 | 0.0041 | 0.0026 | 0.0011 | 0.0011 | 0.0011 | 0.0014 | 0.0007 | 0.0006 | 0.0006 | 0.0014 | 0.0008 | 0.0005 | 0.0022 | 0.0013 |
Peroxisomes | 0.011 | 0.0002 | 0.0023 | 0.0037 | 0.0052 | 0.0003 | 0.01 | 0.0004 | 0.0036 | 0.004 | 0.0005 | 0.0024 | 0.0013 | 0.0004 | 0.0005 | 0.0002 | 0.0003 | 0.0002 |
Punctate Nuclear | 0.0077 | 0.002 | 0.0029 | 0.0007 | 0.0039 | 0.001 | 0.0031 | 0.0014 | 0.0006 | 0.0004 | 0.0004 | 0.0004 | 0.0012 | 0.0012 | 0.0007 | 0.0011 | 0.0012 | 0.0004 |
Vacuole | 0.0112 | 0.0132 | 0.0095 | 0.0095 | 0.0158 | 0.0081 | 0.0074 | 0.0107 | 0.0127 | 0.0138 | 0.004 | 0.0073 | 0.0094 | 0.0078 | 0.0082 | 0.0125 | 0.0079 | 0.0091 |
Vacuole Periphery | 0.0023 | 0.001 | 0.0018 | 0.0022 | 0.0035 | 0.001 | 0.001 | 0.0008 | 0.0018 | 0.0021 | 0.0006 | 0.0009 | 0.0011 | 0.0006 | 0.001 | 0.0015 | 0.0012 | 0.0014 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 36.1772 | 28.7468 | 27.3309 | 41.1241 | 26.0585 | 45.1965 | 33.1533 | 39.8481 | 41.2808 | 35.4753 |
Translational Efficiency | 1.32 | 1.1784 | 1.4106 | 0.9889 | 1.2013 | 1.1711 | 1.286 | 0.9651 | 1.1855 | 1.1024 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
2147 | 1447 | 1065 | 1438 | 1490 | 1478 | 321 | 1697 | 3637 | 2925 | 1386 | 3135 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 888.56 | 1005.65 | 1505.29 | 1413.21 | 786.93 | 1003.51 | 1351.35 | 1442.63 | 846.92 | 1004.57 | 1469.64 | 1429.14 |
Standard Deviation | 120.41 | 186.29 | 235.10 | 218.63 | 128.48 | 151.75 | 176.85 | 245.58 | 133.49 | 169.72 | 232.23 | 234.06 |
Intensity Change Log 2 | — | 0.178587 | 0.760500 | 0.669435 | — | 0.350748 | 0.780094 | 0.874394 | — | 0.262009 | 0.769736 | 0.769332 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000527 | 0.003100 | 0.001914 | 0.007886 | 0.001137 | 0.001505 | 0.000636 | 0.006485 | 0.000777 | 0.002294 | 0.001618 | 0.007127 |
Bud Neck | 0.002099 | 0.001712 | 0.003606 | 0.008877 | 0.003840 | 0.001823 | 0.002647 | 0.005680 | 0.002812 | 0.001768 | 0.003384 | 0.007146 |
Bud Site | 0.007205 | 0.015788 | 0.011191 | 0.049175 | 0.014328 | 0.011533 | 0.008562 | 0.033007 | 0.010123 | 0.013638 | 0.010582 | 0.040423 |
Cell Periphery | 0.001863 | 0.001698 | 0.009671 | 0.012328 | 0.001279 | 0.002313 | 0.006032 | 0.013801 | 0.001624 | 0.002009 | 0.008828 | 0.013125 |
Cytoplasm | 0.107404* | 0.070401 | 0.071565 | 0.080657 | 0.127267* | 0.075812 | 0.042290 | 0.105450* | 0.115542* | 0.073135 | 0.064785 | 0.094077 |
Cytoplasmic Foci | 0.036062 | 0.088529 | 0.006541 | 0.056977 | 0.129176 | 0.053217 | 0.006127 | 0.042604 | 0.074208 | 0.070686 | 0.006445 | 0.049196 |
Eisosomes | 0.000510 | 0.000336 | 0.000093 | 0.000311 | 0.000424 | 0.000456 | 0.000115 | 0.000482 | 0.000475 | 0.000396 | 0.000098 | 0.000404 |
Endoplasmic Reticulum | 0.701913* | 0.619781* | 0.775486* | 0.338899* | 0.444487* | 0.716815* | 0.828906* | 0.469196* | 0.596451* | 0.668812* | 0.787858* | 0.409430* |
Endosome | 0.016508 | 0.045690 | 0.016590 | 0.082767 | 0.049737 | 0.024304 | 0.018676 | 0.043272 | 0.030121 | 0.034883 | 0.017073 | 0.061388 |
Golgi | 0.030330 | 0.040518 | 0.004278 | 0.044173 | 0.056391 | 0.036442 | 0.004007 | 0.036250 | 0.041007 | 0.038459 | 0.004215 | 0.039884 |
Lipid Particles | 0.043215 | 0.036976 | 0.025629 | 0.162675* | 0.061014 | 0.031895 | 0.023429 | 0.182158* | 0.050507 | 0.034409 | 0.025120 | 0.173222* |
Mitochondria | 0.014550 | 0.012764 | 0.000331 | 0.002601 | 0.017300 | 0.010943 | 0.000178 | 0.002609 | 0.015676 | 0.011844 | 0.000296 | 0.002605 |
Mitotic Spindle | 0.000485 | 0.000557 | 0.001213 | 0.019214 | 0.000713 | 0.001270 | 0.000518 | 0.003363 | 0.000579 | 0.000917 | 0.001052 | 0.010634 |
None | 0.005149 | 0.002246 | 0.001589 | 0.001153 | 0.006874 | 0.001235 | 0.000133 | 0.001962 | 0.005856 | 0.001736 | 0.001252 | 0.001591 |
Nuclear Periphery | 0.001002 | 0.001184 | 0.002526 | 0.001302 | 0.002846 | 0.000432 | 0.001204 | 0.000318 | 0.001757 | 0.000804 | 0.002220 | 0.000769 |
Nuclear Periphery Foci | 0.003335 | 0.007647 | 0.025961 | 0.036130 | 0.011423 | 0.003998 | 0.025945 | 0.011674 | 0.006648 | 0.005803 | 0.025957 | 0.022892 |
Nucleolus | 0.000210 | 0.000264 | 0.000022 | 0.000198 | 0.001233 | 0.000119 | 0.000012 | 0.000105 | 0.000629 | 0.000191 | 0.000020 | 0.000147 |
Nucleus | 0.004230 | 0.002213 | 0.002020 | 0.002500 | 0.006804 | 0.002105 | 0.001768 | 0.001878 | 0.005284 | 0.002158 | 0.001961 | 0.002163 |
Peroxisomes | 0.004159 | 0.003944 | 0.000181 | 0.001746 | 0.004076 | 0.004183 | 0.000266 | 0.002683 | 0.004125 | 0.004065 | 0.000200 | 0.002253 |
Vacuole | 0.013288 | 0.030043 | 0.038356 | 0.082343 | 0.040888 | 0.012029 | 0.027611 | 0.032774 | 0.024595 | 0.020941 | 0.035867 | 0.055511 |
Vacuole Periphery | 0.005956 | 0.014609 | 0.001238 | 0.008089 | 0.018763 | 0.007569 | 0.000939 | 0.004251 | 0.011203 | 0.011052 | 0.001169 | 0.006011 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -4.69 | -2.83 | -8.27 | -4.64 | -5.78 | -1.56 | 2.33 | -7.74 | -7.19 | -8.50 | -5.15 | -2.24 | -11.51 | -7.93 | -8.15 |
Bud Neck | 1.21 | -2.91 | -9.30 | -10.06 | -5.48 | 2.64 | 1.14 | -2.39 | -6.15 | -3.49 | 2.76 | -1.58 | -8.59 | -11.53 | -5.50 |
Bud Site | -4.89 | -2.36 | -13.60 | -10.65 | -11.59 | 1.47 | 1.68 | -7.87 | -8.70 | -6.68 | -2.75 | -0.53 | -15.75 | -13.63 | -13.44 |
Cell Periphery | 0.65 | -6.78 | -7.65 | -7.82 | -1.35 | -4.16 | -2.45 | -12.76 | -11.43 | -3.59 | -2.04 | -7.30 | -13.99 | -13.49 | -3.27 |
Cytoplasm | 8.15 | 6.09 | 4.74 | -2.51 | -1.63 | 9.84 | 12.04 | 3.48 | -5.65 | -8.89 | 12.43 | 10.83 | 5.04 | -6.03 | -6.13 |
Cytoplasmic Foci | -13.37 | 14.84 | -6.99 | 7.32 | -18.64 | 15.36 | 29.15 | 18.98 | 3.09 | -15.70 | 1.14 | 30.03 | 9.60 | 7.78 | -23.52 |
Eisosomes | 2.47 | 6.73 | 3.19 | 0.72 | -8.60 | -0.47 | 5.63 | -0.97 | -0.42 | -9.31 | 1.52 | 8.83 | 1.63 | -0.10 | -13.33 |
Endoplasmic Reticulum | 7.21 | -5.96 | 32.75 | 22.41 | 34.31* | -21.67* | -21.81 | -1.67 | 20.74 | 20.98 | -8.39 | -19.05 | 22.04 | 29.55 | 37.66* |
Endosome | -13.27 | -0.29 | -21.85 | -10.38 | -21.40 | 10.39 | 9.22 | 2.38 | -8.55 | -7.67 | -2.99 | 9.02 | -15.95 | -12.70 | -22.63 |
Golgi | -3.88 | 16.19 | -4.52 | -0.84 | -16.18 | 5.87 | 19.34 | 6.17 | 0.13 | -14.35 | 1.21 | 25.23 | 0.78 | -0.40 | -21.97 |
Lipid Particles | 2.09 | 5.15 | -18.88 | -19.79 | -20.99 | 8.40 | 7.42 | -18.25 | -23.74 | -21.72* | 7.19 | 8.98 | -26.80 | -30.84 | -30.85* |
Mitochondria | 1.05 | 12.90 | 9.90 | 7.38 | -4.34 | 3.74 | 14.38 | 11.71 | 6.54 | -6.02 | 3.19 | 18.90 | 15.01 | 9.87 | -7.10 |
Mitotic Spindle | -0.31 | -1.51 | -8.55 | -8.54 | -8.05 | -0.84 | 0.21 | -2.96 | -1.96 | -2.84 | -0.95 | -1.36 | -8.94 | -8.40 | -8.08 |
None | 3.17 | 3.51 | 4.72 | 1.90 | 0.60 | 5.61 | 6.85 | 4.39 | -1.20 | -3.27 | 6.21 | 5.83 | 6.15 | 0.39 | -0.54 |
Nuclear Periphery | -0.36 | -2.43 | -1.43 | -0.31 | 1.87 | 3.76 | 2.26 | 3.93 | 1.11 | 2.69 | 2.59 | -0.88 | 3.30 | 0.03 | 2.96 |
Nuclear Periphery Foci | -4.36 | -11.70 | -13.93 | -11.58 | -3.48 | 5.48 | -3.89 | -0.37 | -6.52 | 3.78 | 1.09 | -11.03 | -12.44 | -12.98 | 1.39 |
Nucleolus | -0.62 | 8.10 | 0.10 | 0.56 | -2.49 | 3.08 | 3.36 | 3.10 | 0.31 | -2.25 | 2.85 | 4.01 | 3.12 | 0.71 | -3.20 |
Nucleus | 5.17 | 5.31 | 3.70 | -1.52 | -1.66 | 6.48 | 4.13 | 6.73 | 0.48 | -0.02 | 8.37 | 7.15 | 7.96 | -0.74 | -0.64 |
Peroxisomes | 0.39 | 13.99 | 7.37 | 4.32 | -8.15 | -0.25 | 12.15 | 3.82 | 4.07 | -10.86 | 0.17 | 18.52 | 7.59 | 5.77 | -13.70 |
Vacuole | -7.00 | -9.34 | -18.14 | -12.57 | -9.89 | 11.55 | 2.64 | 2.32 | -9.52 | -1.12 | 2.21 | -4.98 | -13.63 | -14.93 | -6.66 |
Vacuole Periphery | -8.11 | 10.45 | -2.17 | 5.21 | -8.04 | 8.84 | 15.02 | 12.07 | 4.43 | -5.49 | 0.18 | 18.14 | 7.56 | 6.99 | -10.06 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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