Standard name | |
---|---|
Human Ortholog | |
Description | 3'-5' DNA helicase involved in error-free bypass of DNA lesions; binds flap DNA, stimulates activity of Rad27p and Dna2p; prevents crossovers between ectopic sequences by removing substrates for Mus81-Mms4 or Rad1-Rad10 cleavage; homolog of human FANCM Fanconi anemia protein that is involved in stabilizing and remodeling blocked replication forks; member of SF2 DExD/H superfamily of helicases; nonsense or missense mutations in FANCM can make people more likely to get cancer |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 2 | 2 | 3 | 8 | 7 | 7 | – | 7 | 12 | 0 | 0 | 2 | 0 | 0 | 0 | 3 | 4 | 5 | 4 | 3 | 3 |
Bud Neck | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 2 | 2 |
Bud Site | 0 | 0 | 0 | 2 | 4 | 4 | 10 | 13 | 9 | – | 10 | 14 | 0 | 0 | 1 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Cytoplasm | 18 | 33 | 59 | 52 | 66 | 80 | 89 | 128 | 58 | – | 74 | 69 | 31 | 65 | 74 | 50 | 26 | 28 | 5 | 15 | 24 | 11 | 13 | 23 |
Endoplasmic Reticulum | 3 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | – | 0 | 1 | 0 | 0 | 0 | 11 | 12 | 5 | 0 | 0 | 1 | 1 | 0 | 2 |
Endosome | 2 | 0 | 1 | 0 | 4 | 0 | 2 | 1 | 0 | – | 0 | 1 | 1 | 2 | 1 | 3 | 4 | 4 | 4 | 2 | 3 | 0 | 1 | 9 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 2 | 1 | 3 | 1 | 0 | 1 | 0 | 0 | 0 |
Mitochondria | 0 | 32 | 132 | 139 | 105 | 276 | 320 | 358 | 267 | – | 306 | 343 | 12 | 6 | 57 | 8 | 4 | 4 | 5 | 5 | 19 | 2 | 10 | 14 |
Nucleus | 220 | 134 | 142 | 124 | 127 | 157 | 162 | 213 | 94 | – | 81 | 96 | 120 | 157 | 99 | 192 | 114 | 85 | 223 | 156 | 196 | 125 | 97 | 67 |
Nuclear Periphery | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | – | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 |
Nucleolus | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 3 | 0 | – | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 2 | 2 | 1 | 0 | 2 | 4 | 7 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 2 | 1 | 3 | 5 | 1 | 6 | 4 | – | 2 | 1 | 0 | 3 | 1 | 2 | 1 | 2 | 3 | 3 | 8 | 6 | 9 | 14 |
Vac/Vac Membrane | 45 | 36 | 22 | 11 | 31 | 33 | 25 | 22 | 18 | – | 25 | 35 | 23 | 37 | 58 | 48 | 28 | 22 | 54 | 38 | 113 | 63 | 87 | 116 |
Unique Cell Count | 268 | 199 | 301 | 247 | 273 | 410 | 449 | 549 | 324 | 372 | 414 | 173 | 249 | 258 | 284 | 172 | 140 | 309 | 230 | 386 | 226 | 236 | 271 | |
Labelled Cell Count | 289 | 236 | 360 | 331 | 343 | 560 | 620 | 752 | 457 | 505 | 575 | 188 | 271 | 293 | 318 | 190 | 155 | 309 | 230 | 386 | 226 | 236 | 271 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.6 | 4.1 | 3.7 | 3.7 | 3.9 | 3.4 | 3.3 | 3.4 | 2.8 | – | 2.8 | 2.7 | 4.6 | 4.7 | 4.0 | 6.3 | 6.8 | 7.3 | 4.8 | 4.7 | 5.1 |
Std Deviation (1e-4) | 0.8 | 0.7 | 1.2 | 1.6 | 1.6 | 1.4 | 1.5 | 1.5 | 1.3 | – | 0.8 | 0.9 | 1.2 | 1.1 | 1.0 | 1.5 | 1.6 | 2.3 | 1.5 | 1.3 | 1.6 |
Intensity Change (Log2) | – | – | – | -0.01 | 0.06 | -0.15 | -0.18 | -0.13 | -0.4 | – | -0.42 | -0.45 | 0.31 | 0.32 | 0.09 | 0.75 | 0.86 | 0.97 | 0.37 | 0.34 | 0.47 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | 3.6 | 3.1 | 2.7 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.456 | 1.2714 | 1.2148 | 0.8313 | 0.9167 | 1.0516 | 2.0005 | 2.4442 | 2.3177 | 1.9071 | 2.8659 | 2.2121 | 2.7644 | 3.9394 | 2.9383 | 2.7882 | 2.6441 | 3.1586 |
Actin | 0.064 | 0.0006 | 0.0161 | 0.0001 | 0.0136 | 0.0017 | 0.0169 | 0.0001 | 0.0224 | 0.0075 | 0.0254 | 0.0011 | 0.0034 | 0.0006 | 0.0056 | 0.0004 | 0.0005 | 0.0002 |
Bud | 0.0009 | 0.0002 | 0.001 | 0.0002 | 0.0005 | 0.0016 | 0.0007 | 0.0002 | 0.0004 | 0.0011 | 0.0021 | 0.0001 | 0.0011 | 0.0001 | 0.0004 | 0.0001 | 0.0001 | 0 |
Bud Neck | 0.0058 | 0.0009 | 0.0011 | 0.001 | 0.0015 | 0.0066 | 0.0022 | 0.0006 | 0.001 | 0.003 | 0.0036 | 0.0035 | 0.0003 | 0.0006 | 0.0022 | 0.0008 | 0.0017 | 0.0026 |
Bud Periphery | 0.0022 | 0.0003 | 0.0018 | 0.0014 | 0.002 | 0.0047 | 0.0006 | 0.0002 | 0.0005 | 0.0012 | 0.0019 | 0.0001 | 0.0005 | 0.0001 | 0.0009 | 0.0001 | 0.0001 | 0 |
Bud Site | 0.0048 | 0.0015 | 0.0169 | 0.0007 | 0.001 | 0.0011 | 0.0059 | 0.0015 | 0.0058 | 0.0115 | 0.0142 | 0.0002 | 0.0005 | 0.0005 | 0.0028 | 0.0002 | 0.0004 | 0.0001 |
Cell Periphery | 0.0005 | 0.0002 | 0.0003 | 0.0006 | 0.0004 | 0.0002 | 0.0002 | 0.0001 | 0.0002 | 0.0004 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 |
Cytoplasm | 0.0384 | 0.0787 | 0.0401 | 0.0566 | 0.0298 | 0.0312 | 0.0521 | 0.0275 | 0.0313 | 0.0642 | 0.0207 | 0.0258 | 0.0633 | 0.0372 | 0.0467 | 0.0213 | 0.0486 | 0.0308 |
Cytoplasmic Foci | 0.0158 | 0.004 | 0.007 | 0.0008 | 0.0023 | 0.0059 | 0.0195 | 0.001 | 0.0074 | 0.0335 | 0.0392 | 0.0024 | 0.0019 | 0.0021 | 0.0023 | 0.0003 | 0.0013 | 0.0008 |
Eisosomes | 0.0011 | 0 | 0.0002 | 0 | 0.0007 | 0 | 0.0004 | 0 | 0.0002 | 0.0001 | 0.0003 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0082 | 0.0035 | 0.0061 | 0.0017 | 0.0069 | 0.0024 | 0.0026 | 0.0018 | 0.0068 | 0.0027 | 0.0065 | 0.0012 | 0.003 | 0.0024 | 0.0018 | 0.0009 | 0.0026 | 0.0007 |
Endosome | 0.0261 | 0.0034 | 0.0168 | 0.0059 | 0.0226 | 0.0072 | 0.0162 | 0.0021 | 0.0077 | 0.0272 | 0.021 | 0.0068 | 0.0024 | 0.0018 | 0.0053 | 0.0008 | 0.0027 | 0.0011 |
Golgi | 0.0122 | 0.0002 | 0.0043 | 0.0001 | 0.0058 | 0.0018 | 0.0045 | 0.0001 | 0.0035 | 0.0078 | 0.0206 | 0.0019 | 0.001 | 0.0001 | 0.0009 | 0 | 0.0001 | 0 |
Lipid Particles | 0.0297 | 0.0009 | 0.013 | 0.0005 | 0.0061 | 0.0085 | 0.0068 | 0.0007 | 0.0025 | 0.017 | 0.0063 | 0.0028 | 0.0026 | 0.0002 | 0.0022 | 0 | 0.0002 | 0.0001 |
Mitochondria | 0.0124 | 0.0007 | 0.0085 | 0.0017 | 0.0275 | 0.0013 | 0.0029 | 0.0008 | 0.0033 | 0.0049 | 0.0076 | 0.0034 | 0.0014 | 0.0004 | 0.0014 | 0.0011 | 0.0005 | 0.0003 |
None | 0.0915 | 0.0469 | 0.0662 | 0.0294 | 0.0256 | 0.038 | 0.1181 | 0.0844 | 0.0668 | 0.0749 | 0.1181 | 0.0761 | 0.1005 | 0.0558 | 0.0817 | 0.0384 | 0.0621 | 0.0662 |
Nuclear Periphery | 0.0334 | 0.0209 | 0.0253 | 0.0098 | 0.0375 | 0.0152 | 0.0189 | 0.0165 | 0.024 | 0.0152 | 0.0159 | 0.013 | 0.0104 | 0.0272 | 0.0178 | 0.0123 | 0.0259 | 0.0066 |
Nucleolus | 0.0085 | 0.0101 | 0.008 | 0.0082 | 0.0086 | 0.017 | 0.0099 | 0.0124 | 0.007 | 0.0081 | 0.0065 | 0.0167 | 0.0045 | 0.0069 | 0.0101 | 0.0074 | 0.0093 | 0.0132 |
Nucleus | 0.5697 | 0.7982 | 0.732 | 0.8188 | 0.7692 | 0.8178 | 0.6452 | 0.817 | 0.7691 | 0.5838 | 0.6272 | 0.8006 | 0.7888 | 0.8045 | 0.779 | 0.8483 | 0.8114 | 0.8218 |
Peroxisomes | 0.023 | 0.0003 | 0.006 | 0.0001 | 0.0014 | 0.0027 | 0.0071 | 0.0001 | 0.0059 | 0.0516 | 0.0099 | 0.0027 | 0.0004 | 0.0001 | 0.0033 | 0.0001 | 0.0001 | 0 |
Punctate Nuclear | 0.0441 | 0.0217 | 0.021 | 0.0314 | 0.0316 | 0.0329 | 0.0655 | 0.0299 | 0.0322 | 0.0744 | 0.0509 | 0.0404 | 0.0116 | 0.057 | 0.0268 | 0.0668 | 0.0283 | 0.0545 |
Vacuole | 0.0057 | 0.0059 | 0.0057 | 0.0265 | 0.0027 | 0.0018 | 0.0027 | 0.0022 | 0.0016 | 0.0087 | 0.0012 | 0.0008 | 0.0019 | 0.0019 | 0.0057 | 0.0004 | 0.0035 | 0.0007 |
Vacuole Periphery | 0.0021 | 0.0007 | 0.0024 | 0.0045 | 0.0029 | 0.0004 | 0.001 | 0.0006 | 0.0006 | 0.0012 | 0.0008 | 0.0003 | 0.0003 | 0.0004 | 0.0029 | 0.0002 | 0.0007 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 7.0304 | 9.9233 | 7.1181 | 9.6405 | 9.3551 | 5.9171 | 9.8662 | 8.9163 | 8.8961 | 9.6984 |
Translational Efficiency | – | – | – | – | – | – | – | – | – | – |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | – |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1835 | 97 | 1381 | 928 | 1704 | 1749 | 139 | 152 | 3539 | 1846 | 1520 | 1080 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 603.60 | 783.78 | 885.71 | 876.54 | 678.46 | 748.10 | 988.04 | 915.95 | 639.64 | 749.97 | 895.07 | 882.09 |
Standard Deviation | 76.90 | 103.69 | 101.57 | 124.99 | 79.25 | 95.16 | 113.39 | 114.48 | 86.54 | 95.95 | 106.86 | 124.32 |
Intensity Change Log 2 | — | 0.376856 | 0.553242 | 0.538227 | — | 0.140967 | 0.542306 | 0.433005 | — | 0.256840 | 0.547465 | 0.483501 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000142 | 0.000712 | 0.000265 | 0.004137 | 0.000049 | 0.000195 | 0.000637 | 0.000696 | 0.000097 | 0.000222 | 0.000299 | 0.003653 |
Bud Neck | 0.005343 | 0.002440 | 0.004807 | 0.014675 | 0.002323 | 0.006629 | 0.003219 | 0.005593 | 0.003889 | 0.006409 | 0.004662 | 0.013397 |
Bud Site | 0.001079 | 0.000970 | 0.004394 | 0.024134 | 0.000417 | 0.005606 | 0.000780 | 0.004764 | 0.000760 | 0.005362 | 0.004064 | 0.021408 |
Cell Periphery | 0.000153 | 0.000142 | 0.000069 | 0.000208 | 0.000110 | 0.000152 | 0.000088 | 0.000132 | 0.000132 | 0.000151 | 0.000071 | 0.000197 |
Cytoplasm | 0.291535* | 0.087634 | 0.020247 | 0.075123 | 0.149933* | 0.142570* | 0.006617 | 0.058010 | 0.223355* | 0.139683* | 0.019001 | 0.072714 |
Cytoplasmic Foci | 0.056605 | 0.024480 | 0.000226 | 0.006431 | 0.022876 | 0.026748 | 0.000212 | 0.000467 | 0.040365 | 0.026629 | 0.000225 | 0.005592 |
Eisosomes | 0.000056 | 0.000075 | 0.000015 | 0.000031 | 0.000027 | 0.000039 | 0.000047 | 0.000029 | 0.000042 | 0.000041 | 0.000018 | 0.000031 |
Endoplasmic Reticulum | 0.000747 | 0.003181 | 0.004890 | 0.008097 | 0.000923 | 0.001707 | 0.012568 | 0.010223 | 0.000832 | 0.001784 | 0.005592 | 0.008396 |
Endosome | 0.001534 | 0.002426 | 0.000396 | 0.005941 | 0.000804 | 0.004018 | 0.000590 | 0.000704 | 0.001182 | 0.003934 | 0.000414 | 0.005204 |
Golgi | 0.000442 | 0.000169 | 0.000025 | 0.006827 | 0.000359 | 0.001391 | 0.000042 | 0.000054 | 0.000402 | 0.001327 | 0.000027 | 0.005874 |
Lipid Particles | 0.005652 | 0.005155 | 0.000243 | 0.000510 | 0.003418 | 0.003669 | 0.000608 | 0.000477 | 0.004576 | 0.003747 | 0.000276 | 0.000505 |
Mitochondria | 0.002478 | 0.002927 | 0.000797 | 0.010161 | 0.001890 | 0.005331 | 0.000580 | 0.001027 | 0.002195 | 0.005204 | 0.000777 | 0.008875 |
Mitotic Spindle | 0.000553 | 0.000079 | 0.000847 | 0.029768 | 0.000655 | 0.009340 | 0.000117 | 0.002893 | 0.000602 | 0.008853 | 0.000780 | 0.025985 |
None | 0.014977 | 0.004627 | 0.001679 | 0.015030 | 0.010106 | 0.008280 | 0.001491 | 0.005474 | 0.012631 | 0.008088 | 0.001662 | 0.013685 |
Nuclear Periphery | 0.000832 | 0.001004 | 0.001177 | 0.002396 | 0.001036 | 0.001609 | 0.001090 | 0.001730 | 0.000930 | 0.001577 | 0.001169 | 0.002302 |
Nuclear Periphery Foci | 0.000362 | 0.000563 | 0.000159 | 0.000696 | 0.000250 | 0.000506 | 0.000266 | 0.000465 | 0.000308 | 0.000509 | 0.000168 | 0.000663 |
Nucleolus | 0.002848 | 0.002168 | 0.000606 | 0.001287 | 0.003033 | 0.004882 | 0.001192 | 0.000853 | 0.002937 | 0.004740 | 0.000660 | 0.001226 |
Nucleus | 0.602701* | 0.839197* | 0.953561* | 0.765692* | 0.797079* | 0.764334* | 0.957239* | 0.891499* | 0.696292* | 0.768268* | 0.953898* | 0.783398* |
Peroxisomes | 0.002748 | 0.003139 | 0.000114 | 0.003161 | 0.001696 | 0.003021 | 0.000150 | 0.000096 | 0.002242 | 0.003027 | 0.000117 | 0.002730 |
Vacuole | 0.008997 | 0.017095 | 0.005391 | 0.021271 | 0.002861 | 0.009283 | 0.012369 | 0.014675 | 0.006042 | 0.009693 | 0.006029 | 0.020342 |
Vacuole Periphery | 0.000217 | 0.001818 | 0.000092 | 0.004425 | 0.000156 | 0.000693 | 0.000096 | 0.000139 | 0.000188 | 0.000752 | 0.000092 | 0.003822 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -1.13 | -2.62 | -3.09 | -1.80 | -2.85 | -5.40 | -7.37 | -6.96 | -6.13 | -1.98 | -2.69 | -6.17 | -3.45 | -3.24 | -3.00 |
Bud Neck | 2.67 | -0.72 | -7.51 | -9.24 | -7.39 | -4.81 | -3.92 | -5.08 | -2.27 | -3.11 | -2.84 | -2.91 | -9.33 | -6.96 | -7.72 |
Bud Site | -0.03 | -2.81 | -5.79 | -5.74 | -3.41 | -4.83 | -4.53 | -1.99 | -0.90 | -1.76 | -4.52 | -3.11 | -6.24 | -3.42 | -3.66 |
Cell Periphery | 0.16 | 4.14 | -0.68 | -0.59 | -7.58 | -2.23 | 0.47 | -2.39 | -0.34 | -2.52 | -1.06 | 4.89 | -2.33 | -0.72 | -8.07 |
Cytoplasm | 12.88 | 46.09 | 35.46 | 2.25 | -7.71 | 0.98 | 27.07 | 12.41 | 11.82 | -4.31 | 13.87 | 48.34 | 33.45 | 16.08 | -8.40 |
Cytoplasmic Foci | 4.65 | 22.09 | 20.50 | 3.52 | -2.84 | -2.02 | 13.87 | 13.64 | 15.61 | -2.25 | 5.33 | 25.65 | 22.83 | 14.32 | -2.90 |
Eisosomes | -1.51 | 11.85 | 1.42 | 1.85 | -13.04 | -4.55 | -4.39 | -5.66 | -3.27 | 0.37 | -0.08 | 9.71 | -3.30 | -2.84 | -12.31 |
Endoplasmic Reticulum | -3.31 | -15.77 | -19.56 | -9.44 | -9.98 | -4.45 | -8.26 | -9.21 | -8.70 | -0.61 | -5.87 | -17.34 | -21.50 | -19.09 | -9.84 |
Endosome | -0.80 | 6.49 | -1.88 | -0.21 | -4.17 | -5.85 | 0.35 | -2.14 | 4.99 | -2.43 | -5.22 | 6.51 | -2.64 | 2.30 | -4.34 |
Golgi | 2.36 | 4.08 | -1.88 | -2.20 | -2.35 | -2.23 | 2.24 | 1.62 | 2.84 | -2.43 | -2.15 | 4.53 | -1.88 | -0.67 | -2.37 |
Lipid Particles | 0.30 | 7.65 | 6.91 | 2.33 | -5.25 | -0.70 | 5.19 | 5.05 | 8.61 | -0.52 | 1.15 | 9.71 | 8.59 | 9.34 | -5.45 |
Mitochondria | -0.11 | 3.14 | -1.68 | -0.92 | -2.96 | -3.31 | 2.13 | 0.40 | 3.89 | -2.70 | -3.24 | 3.45 | -1.84 | 0.53 | -3.06 |
Mitotic Spindle | 1.31 | -1.50 | -6.58 | -6.75 | -6.22 | -4.99 | 0.02 | -2.60 | -0.04 | -2.60 | -5.07 | -1.47 | -6.89 | -3.76 | -6.51 |
None | 5.87 | 9.83 | 3.16 | -2.69 | -5.87 | 1.30 | 8.03 | 1.38 | 0.53 | -2.29 | 3.35 | 13.06 | 2.74 | -0.50 | -6.29 |
Nuclear Periphery | -1.16 | -5.96 | -14.81 | -10.70 | -10.65 | -3.84 | -2.91 | -4.93 | -3.47 | -3.89 | -4.78 | -5.61 | -15.29 | -9.41 | -11.24 |
Nuclear Periphery Foci | -0.88 | 2.32 | -5.43 | -1.86 | -6.79 | -3.12 | -1.38 | -3.70 | -2.27 | -2.63 | -2.76 | 1.68 | -6.75 | -3.76 | -7.39 |
Nucleolus | 1.64 | 5.40 | 1.74 | -0.07 | -5.35 | -2.64 | 4.40 | 2.42 | 4.50 | -1.25 | -2.94 | 7.78 | 2.73 | 4.61 | -5.60 |
Nucleus | -9.41 | -45.87 | -18.21 | 1.26 | 16.23 | 3.95 | -12.62 | -2.49 | -4.44 | 4.92 | -8.30 | -43.55 | -12.13 | -4.14 | 17.01 |
Peroxisomes | -0.18 | 6.21 | 2.39 | 1.24 | -1.72 | -2.68 | 4.54 | 4.56 | 7.88 | 0.12 | -1.74 | 7.71 | 2.34 | 3.46 | -1.72 |
Vacuole | -1.92 | 1.02 | -10.47 | -2.05 | -10.80 | -8.68 | -3.25 | -5.09 | -4.39 | -2.16 | -4.61 | -3.09 | -12.46 | -10.84 | -10.95 |
Vacuole Periphery | -1.07 | 3.95 | -2.44 | 0.10 | -2.65 | -4.90 | 1.85 | -0.73 | 4.61 | -2.36 | -4.47 | 3.84 | -2.49 | -1.23 | -2.67 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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