Standard name
Human Ortholog
Description Component of the U2 snRNP; required for the first catalytic step of splicing and for spliceosomal assembly; interacts with Rds3p and is required for Mer1p-activated splicing; diploid mutants have a specific defect in MATa1 pre-mRNA splicing which leads to haploid gene expression in diploids
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0.05 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 – 0 0 0 0 0 0.07 0.13 0.14 0.12 0.11 0.12 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.07 0 0.2 0.29 0.13 0.36 – 0.36 0.57 0.53 0.61 0.58 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.93 0.95 0.91 0.91 0.9 0.87 – 0.81 0.79 0.83 0.78 0.81 0.92 0.85 0.8 0.82 0.82 0.76 0.96 0.93 0.93 0.88 0.8 0.68
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0.05 – 0.06 0.06 0.07 0.05 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.09 0.06 0.1 0 0 0 0.06 0.13 0.22
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 1 1 2 – 5 2 11 6 7 0 1 0 0 0 0 0 0 1 0 2 3
Bud Neck 0 0 0 0 0 0 – 0 0 0 1 0 0 0 0 0 0 1 0 0 0 0 0 0
Bud Site 0 0 0 1 1 9 – 24 12 26 27 27 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0
Cytoplasm 0 1 5 14 24 20 – 27 20 15 22 15 14 42 53 27 28 18 0 1 0 1 1 0
Endoplasmic Reticulum 0 0 0 0 1 0 – 0 0 0 0 0 1 0 0 5 9 4 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 1 0 0 0 0 7 1 1 0 0 0 0 0 3 2
Golgi 0 0 0 0 0 1 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1
Mitochondria 8 2 25 95 72 176 – 297 259 360 347 418 5 2 4 5 6 3 0 0 0 0 2 2
Nucleus 114 106 117 299 494 421 – 664 355 568 448 582 180 277 293 182 215 117 117 107 120 51 239 228
Nuclear Periphery 0 0 0 1 4 11 – 17 17 17 12 14 0 10 12 2 0 1 0 0 0 0 0 0
Nucleolus 1 0 1 4 5 23 – 50 27 45 30 44 0 2 6 1 5 1 0 0 0 0 3 14
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 2 1 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 6 0 2 2 1 0 1 3 1 0 1 0 0 0 0 1 3
Vac/Vac Membrane 5 3 1 6 10 6 – 15 2 3 0 6 3 5 11 19 15 15 3 2 3 3 39 74
Unique Cell Count 122 112 128 330 548 484 821 452 684 573 715 196 325 367 223 262 154 122 115 130 59 298 337
Labelled Cell Count 128 112 149 421 613 669 1105 694 1048 895 1114 203 340 392 244 279 162 122 115 130 59 298 337


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.4 9.0 5.7 5.3 5.6 4.6 – 4.4 3.8 3.9 3.9 3.6 7.3 7.5 7.7 10.1 10.1 10.5 6.3 7.7 7.8
Std Deviation (1e-4) 1.4 1.9 1.1 1.2 1.3 1.3 – 1.4 1.5 1.1 1.8 1.1 1.7 1.8 2.5 2.3 2.4 2.6 1.1 1.6 1.7
Intensity Change (Log2) – – – -0.1 -0.02 -0.31 – -0.36 -0.55 -0.54 -0.55 -0.66 0.36 0.41 0.44 0.84 0.84 0.9 0.16 0.45 0.46

WT3RAP60RAP140RAP220RAP380RAP460RAP540RAP620RAP7000510WT3HU80HU120HU1600510WT3rpd3Δ_1rpd3Δ_2rpd3Δ_30510WT1AF100AF140AF1800510
rapamycinhydroxy-urearpd3 knockoutalpha-factorMultiple Condition Time-Course Intensity PlotsGFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.2 0.2 0.1 – 0 0.3 0 0 0 1.2 2.8 3.2 2.6 2.3 2.4
Endoplasmic Reticulum 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 2.0 -1.9 0 – 0 0 0 0 0 -5.2 -7.7 -7.6 -5.6 -5.9 -4.9
Nucleus -0.3 -0.4 -1.4 – -2.9 -3.3 -2.4 -3.4 -2.8 0.1 -1.8 -3.0 -2.5 -2.4 -3.4
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 – 0 0 0 0 0 0 0 0 3.0 2.3 3.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.1452 2.6638 2.2353 1.8184 1.8413 2.1279 1.223 2.0503 1.2208 1.322 1.4531 1.4754 2.8338 4.1622 3.6177 3.1753 3.0343 3.3118
Actin 0.0152 0.0001 0.0029 0.0327 0.0122 0.0066 0.0218 0 0.0017 0.0354 0.0008 0.0009 0 0 0 0 0 0
Bud 0.0012 0.0003 0.0001 0.0007 0.001 0.0003 0.0004 0 0.0001 0.0007 0.0003 0.0002 0 0 0 0 0 0
Bud Neck 0.0034 0.0008 0.0027 0.0018 0.0017 0.0014 0.0013 0.0027 0.0014 0.0003 0.0003 0.0009 0 0.0001 0.0001 0.0001 0.0007 0.0004
Bud Periphery 0.0023 0.0003 0.0002 0.0015 0.0036 0.0007 0.0009 0.0001 0.0002 0.0007 0.0002 0.0001 0 0 0 0 0 0
Bud Site 0.0057 0.0027 0.0013 0.0017 0.001 0.0004 0.0023 0.0001 0.0004 0.0042 0.0001 0.0001 0 0 0.0002 0 0 0
Cell Periphery 0.0004 0.0001 0.0001 0.0004 0.0003 0.0001 0.0002 0.0003 0.0001 0.0001 0 0 0 0 0 0 0 0
Cytoplasm 0.0151 0.0061 0.0077 0.0056 0.0042 0.0123 0.0055 0.0061 0.0058 0.0013 0.0112 0.0046 0.0002 0.0006 0.0052 0.0002 0.0001 0.001
Cytoplasmic Foci 0.0221 0.0038 0.0072 0.0009 0.0118 0.0065 0.0233 0.0001 0.0044 0.0053 0.0065 0.0009 0 0 0.0003 0 0 0
Eisosomes 0.0001 0 0 0.0002 0.0002 0 0.0002 0 0 0.0004 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0036 0.001 0.0008 0.0018 0.0027 0.0004 0.0037 0.0004 0.0003 0.0008 0.0029 0.0004 0 0 0.0001 0 0 0
Endosome 0.0382 0.0121 0.0166 0.0042 0.0156 0.0176 0.0235 0.0002 0.0058 0.0045 0.0222 0.0023 0 0.0001 0.0003 0 0 0
Golgi 0.0067 0.0008 0.0032 0.004 0.0119 0.0045 0.0079 0 0.0055 0.0031 0.0085 0.0014 0 0 0 0 0 0
Lipid Particles 0.0157 0.0006 0.011 0.0038 0.0456 0.0017 0.0239 0 0.0029 0.006 0.0039 0.0029 0 0 0 0 0 0
Mitochondria 0.0079 0.001 0.0027 0.0033 0.0832 0.0116 0.0109 0.0002 0.0184 0.0039 0.0019 0.0017 0.0001 0.0001 0.0002 0.0001 0.0001 0.0001
None 0.0027 0.0003 0.0015 0.0013 0.0009 0.0003 0.002 0.0241 0.004 0.0122 0.0004 0.001 0 0 0.0031 0 0 0.0072
Nuclear Periphery 0.0154 0.0076 0.0039 0.0196 0.0226 0.0043 0.0235 0.0077 0.0079 0.0326 0.0324 0.0107 0.0006 0.0036 0.0029 0.0013 0.0004 0.0004
Nucleolus 0.0092 0.0079 0.0083 0.0082 0.0173 0.0156 0.0055 0.0116 0.0065 0.0051 0.0042 0.0105 0.0042 0.0038 0.0074 0.0053 0.0122 0.0093
Nucleus 0.7939 0.9345 0.9145 0.8996 0.7071 0.9109 0.8124 0.9446 0.9225 0.8655 0.8978 0.9551 0.9946 0.9911 0.9766 0.9922 0.9861 0.9795
Peroxisomes 0.0129 0.0002 0.0102 0.0006 0.0214 0.0008 0.0105 0 0.0032 0.0042 0.0007 0.0006 0 0 0 0 0 0
Punctate Nuclear 0.0168 0.0133 0.003 0.0022 0.0127 0.0015 0.0137 0.0008 0.0073 0.011 0.0022 0.0045 0.0002 0.0004 0.0034 0.0004 0.0002 0.0017
Vacuole 0.0087 0.0058 0.0014 0.0045 0.0171 0.0019 0.0038 0.0008 0.001 0.0008 0.0009 0.0007 0 0.0001 0.0001 0.0002 0.0001 0.0001
Vacuole Periphery 0.0028 0.0007 0.0006 0.0014 0.0058 0.0007 0.0028 0.0001 0.0005 0.002 0.0026 0.0005 0 0 0 0 0 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 8.6396 4.4133 4.9123 9.7579 11.2017 2.4671 8.3668 12.7999 8.2536 10.7614
Translational Efficiency 1.7462 2.9955 2.8916 1.5561 1.206 7.771 2.3259 1.5439 2.7385 1.9486

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1684 939 192 1216 2044 1343 1404 1215 3728 2282 1596 2431

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 734.91 865.83 1103.77 847.31 787.31 983.03 831.64 812.85 763.64 934.80 864.38 830.09
Standard Deviation 366.51 152.86 151.76 177.94 154.65 164.58 111.02 105.81 272.90 169.94 146.46 147.41
Intensity Change Log 2 — 0.236516 0.586800 0.205322 — 0.320304 0.079027 0.046057 — 0.280459 0.346462 0.125145

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000180 0.000569 0.001313 0.001714 0.000159 0.000448 0.000466 0.000663 0.000168 0.000498 0.000568 0.001188
Bud Neck 0.001781 0.008304 0.008352 0.020275 0.003207 0.009575 0.011427 0.011803 0.002563 0.009052 0.011057 0.016041
Bud Site 0.000882 0.001799 0.007163 0.026362 0.000571 0.001234 0.004557 0.015691 0.000711 0.001466 0.004870 0.021029
Cell Periphery 0.000378 0.000329 0.000080 0.000387 0.000261 0.000162 0.000158 0.000262 0.000314 0.000230 0.000149 0.000325
Cytoplasm 0.010326 0.003890 0.002144 0.073905 0.002261 0.005078 0.023533 0.071054 0.005904 0.004589 0.020960 0.072480
Cytoplasmic Foci 0.000458 0.001312 0.000082 0.004130 0.000173 0.001022 0.001049 0.002656 0.000302 0.001142 0.000932 0.003393
Eisosomes 0.000018 0.000025 0.000044 0.000066 0.000018 0.000022 0.000023 0.000030 0.000018 0.000023 0.000026 0.000048
Endoplasmic Reticulum 0.006354 0.008499 0.009214 0.008109 0.005745 0.007262 0.004684 0.006678 0.006020 0.007771 0.005229 0.007394
Endosome 0.000223 0.000726 0.000296 0.007068 0.000244 0.000512 0.001387 0.002840 0.000235 0.000600 0.001255 0.004955
Golgi 0.000036 0.000786 0.000085 0.008407 0.000044 0.000198 0.000091 0.001872 0.000041 0.000440 0.000090 0.005141
Lipid Particles 0.000084 0.000341 0.000238 0.000869 0.000112 0.000387 0.000470 0.001391 0.000099 0.000368 0.000442 0.001130
Mitochondria 0.000419 0.004128 0.001370 0.037308 0.001184 0.003063 0.001742 0.004442 0.000839 0.003501 0.001698 0.020882
Mitotic Spindle 0.000025 0.002079 0.000124 0.038072 0.000249 0.007337 0.004571 0.010026 0.000148 0.005173 0.004036 0.024055
None 0.022077 0.004158 0.001323 0.006326 0.004218 0.002342 0.006369 0.005467 0.012285 0.003089 0.005762 0.005896
Nuclear Periphery 0.000287 0.000451 0.000426 0.002592 0.000753 0.001265 0.000933 0.002322 0.000543 0.000930 0.000872 0.002457
Nuclear Periphery Foci 0.000182 0.000298 0.000241 0.000795 0.000209 0.000349 0.000273 0.000577 0.000197 0.000328 0.000270 0.000686
Nucleolus 0.005553 0.004807 0.003208 0.001442 0.003906 0.003840 0.002582 0.001216 0.004650 0.004238 0.002657 0.001329
Nucleus 0.949591 0.955668 0.959771 0.731884 0.975697 0.953442 0.927932 0.840131 0.963904 0.954358 0.931762 0.785985
Peroxisomes 0.000098 0.000606 0.000339 0.000718 0.000180 0.000585 0.000234 0.000491 0.000143 0.000594 0.000247 0.000604
Vacuole 0.000966 0.000969 0.004038 0.024433 0.000606 0.001530 0.006845 0.018548 0.000769 0.001299 0.006507 0.021492
Vacuole Periphery 0.000082 0.000256 0.000150 0.005138 0.000203 0.000347 0.000673 0.001841 0.000148 0.000310 0.000610 0.003490

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -5.81 -11.03 -6.45 -4.12 0.73 -5.89 -9.57 -5.92 -2.07 -1.55 -8.25 -13.17 -8.40 -4.68 -3.36
Bud Neck -5.58 -5.97 -10.39 -4.88 -4.66 -8.55 -8.15 -8.56 -1.89 -0.08 -10.02 -9.47 -13.31 -5.12 -2.50
Bud Site -1.83 -1.21 -6.70 -6.26 -2.06 -3.02 -5.21 -5.12 -4.72 -2.83 -3.14 -4.75 -8.43 -7.87 -4.99
Cell Periphery 0.53 5.43 2.04 0.83 -4.35 1.47 2.03 0.91 -0.52 -0.92 1.50 4.29 1.88 0.11 -2.45
Cytoplasm 3.65 4.61 -13.62 -15.67 -16.09 -3.04 -10.61 -17.06 -15.74 -10.15 1.10 -8.01 -21.84 -22.16 -14.18
Cytoplasmic Foci -0.98 3.19 -4.04 -1.19 -5.09 -1.74 -2.73 -4.66 -1.54 -2.11 -1.87 -2.15 -6.16 -1.97 -3.32
Eisosomes -2.76 -8.17 -6.78 -5.55 -1.54 -2.08 -4.37 -8.65 -7.21 -5.68 -3.36 -7.23 -9.54 -7.83 -5.98
Endoplasmic Reticulum -3.01 -3.62 -5.60 -2.66 -0.01 -4.13 0.21 -5.79 -2.65 -5.97 -4.82 -0.60 -8.13 -4.01 -7.52
Endosome -2.44 -2.08 -4.48 -3.68 -4.29 -3.37 -2.56 -3.72 -3.07 -1.28 -3.82 -2.63 -5.77 -4.81 -3.03
Golgi -1.80 -4.45 -3.13 -2.28 -3.07 -1.96 -1.86 -1.75 -1.44 -1.66 -2.26 -2.61 -3.58 -2.80 -3.49
Lipid Particles -1.63 -4.90 -7.85 -3.22 -5.56 -2.30 -3.81 -3.25 -2.23 -2.00 -2.82 -4.27 -5.53 -3.47 -3.03
Mitochondria -2.31 -3.78 -5.35 -3.72 -4.96 -1.81 -1.25 -2.52 -0.25 -2.05 -2.97 -2.97 -5.78 -3.71 -5.22
Mitotic Spindle -2.06 -2.22 -7.69 -6.67 -7.65 -3.83 -3.81 -4.87 -0.64 -2.11 -4.33 -3.90 -9.02 -5.48 -6.30
None 7.00 8.57 6.36 -1.95 -6.74 1.87 -1.24 -0.19 -2.93 1.35 7.16 5.11 5.49 -3.65 -0.22
Nuclear Periphery -3.39 -3.71 -19.27 -18.14 -17.51 -1.83 -2.55 -9.17 -6.18 -8.61 -2.34 -5.22 -17.73 -12.92 -15.33
Nuclear Periphery Foci -1.32 -0.83 -4.73 -3.27 -2.77 -1.22 -1.57 -3.89 -2.47 -2.29 -1.73 -1.90 -6.02 -3.94 -3.23
Nucleolus 1.11 3.66 7.93 5.58 3.69 -0.01 1.45 5.88 5.22 2.98 0.79 2.98 9.54 7.38 2.97
Nucleus -0.97 -0.91 19.86 19.83 17.90 5.97 11.27 20.46 15.30 11.17 3.41 8.59 28.38 24.85 18.82
Peroxisomes -2.50 -7.78 -3.47 0.83 -0.46 -1.84 -0.41 -1.18 1.12 -1.01 -2.91 -1.75 -2.92 1.35 -1.79
Vacuole -0.27 -4.90 -13.67 -13.66 -11.94 -5.41 -7.62 -13.04 -12.53 -9.06 -4.33 -7.97 -18.83 -18.45 -13.65
Vacuole Periphery -1.62 -3.48 -3.24 -2.91 -3.10 -1.33 -1.63 -1.45 -1.19 -0.76 -2.15 -1.97 -3.38 -2.97 -2.33
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Component of the U2 snRNP; required for the first catalytic step of splicing and for spliceosomal assembly; interacts with Rds3p and is required for Mer1p-activated splicing; diploid mutants have a specific defect in MATa1 pre-mRNA splicing which leads to haploid gene expression in diploids
Localization
Cell Percentages nucleus (100%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Ist3

Ist3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ist3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available