Standard name
Human Ortholog
Description Component of the U2 snRNP; required for the first catalytic step of splicing and for spliceosomal assembly; interacts with Rds3p and is required for Mer1p-activated splicing; diploid mutants have a specific defect in MATa1 pre-mRNA splicing which leads to haploid gene expression in diploids
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0.05 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 – 0 0 0 0 0 0.07 0.13 0.14 0.12 0.11 0.12 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.07 0 0.2 0.29 0.13 0.36 – 0.36 0.57 0.53 0.61 0.58 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.93 0.95 0.91 0.91 0.9 0.87 – 0.81 0.79 0.83 0.78 0.81 0.92 0.85 0.8 0.82 0.82 0.76 0.96 0.93 0.93 0.88 0.8 0.68
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0.05 – 0.06 0.06 0.07 0.05 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.09 0.06 0.1 0 0 0 0.06 0.13 0.22
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 1 1 2 – 5 2 11 6 7 0 1 0 0 0 0 0 0 1 0 2 3
Bud Neck 0 0 0 0 0 0 – 0 0 0 1 0 0 0 0 0 0 1 0 0 0 0 0 0
Bud Site 0 0 0 1 1 9 – 24 12 26 27 27 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0
Cytoplasm 0 1 5 14 24 20 – 27 20 15 22 15 14 42 53 27 28 18 0 1 0 1 1 0
Endoplasmic Reticulum 0 0 0 0 1 0 – 0 0 0 0 0 1 0 0 5 9 4 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 1 0 0 0 0 7 1 1 0 0 0 0 0 3 2
Golgi 0 0 0 0 0 1 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1
Mitochondria 8 2 25 95 72 176 – 297 259 360 347 418 5 2 4 5 6 3 0 0 0 0 2 2
Nucleus 114 106 117 299 494 421 – 664 355 568 448 582 180 277 293 182 215 117 117 107 120 51 239 228
Nuclear Periphery 0 0 0 1 4 11 – 17 17 17 12 14 0 10 12 2 0 1 0 0 0 0 0 0
Nucleolus 1 0 1 4 5 23 – 50 27 45 30 44 0 2 6 1 5 1 0 0 0 0 3 14
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 2 1 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 6 0 2 2 1 0 1 3 1 0 1 0 0 0 0 1 3
Vac/Vac Membrane 5 3 1 6 10 6 – 15 2 3 0 6 3 5 11 19 15 15 3 2 3 3 39 74
Unique Cell Count 122 112 128 330 548 484 821 452 684 573 715 196 325 367 223 262 154 122 115 130 59 298 337
Labelled Cell Count 128 112 149 421 613 669 1105 694 1048 895 1114 203 340 392 244 279 162 122 115 130 59 298 337


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.4 9.0 5.7 5.3 5.6 4.6 – 4.4 3.8 3.9 3.9 3.6 7.3 7.5 7.7 10.1 10.1 10.5 6.3 7.7 7.8
Std Deviation (1e-4) 1.4 1.9 1.1 1.2 1.3 1.3 – 1.4 1.5 1.1 1.8 1.1 1.7 1.8 2.5 2.3 2.4 2.6 1.1 1.6 1.7
Intensity Change (Log2) – – – -0.1 -0.02 -0.31 – -0.36 -0.55 -0.54 -0.55 -0.66 0.36 0.41 0.44 0.84 0.84 0.9 0.16 0.45 0.46


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.2 0.2 0.1 – 0 0.3 0 0 0 1.2 2.8 3.2 2.6 2.3 2.4
Endoplasmic Reticulum 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 2.0 -1.9 0 – 0 0 0 0 0 -5.2 -7.7 -7.6 -5.6 -5.9 -4.9
Nucleus -0.3 -0.4 -1.4 – -2.9 -3.3 -2.4 -3.4 -2.8 0.1 -1.8 -3.0 -2.5 -2.4 -3.4
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 – 0 0 0 0 0 0 0 0 3.0 2.3 3.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.1452 2.6638 2.2353 1.8184 1.8413 2.1279 1.223 2.0503 1.2208 1.322 1.4531 1.4754 2.8338 4.1622 3.6177 3.1753 3.0343 3.3118
Actin 0.0152 0.0001 0.0029 0.0327 0.0122 0.0066 0.0218 0 0.0017 0.0354 0.0008 0.0009 0 0 0 0 0 0
Bud 0.0012 0.0003 0.0001 0.0007 0.001 0.0003 0.0004 0 0.0001 0.0007 0.0003 0.0002 0 0 0 0 0 0
Bud Neck 0.0034 0.0008 0.0027 0.0018 0.0017 0.0014 0.0013 0.0027 0.0014 0.0003 0.0003 0.0009 0 0.0001 0.0001 0.0001 0.0007 0.0004
Bud Periphery 0.0023 0.0003 0.0002 0.0015 0.0036 0.0007 0.0009 0.0001 0.0002 0.0007 0.0002 0.0001 0 0 0 0 0 0
Bud Site 0.0057 0.0027 0.0013 0.0017 0.001 0.0004 0.0023 0.0001 0.0004 0.0042 0.0001 0.0001 0 0 0.0002 0 0 0
Cell Periphery 0.0004 0.0001 0.0001 0.0004 0.0003 0.0001 0.0002 0.0003 0.0001 0.0001 0 0 0 0 0 0 0 0
Cytoplasm 0.0151 0.0061 0.0077 0.0056 0.0042 0.0123 0.0055 0.0061 0.0058 0.0013 0.0112 0.0046 0.0002 0.0006 0.0052 0.0002 0.0001 0.001
Cytoplasmic Foci 0.0221 0.0038 0.0072 0.0009 0.0118 0.0065 0.0233 0.0001 0.0044 0.0053 0.0065 0.0009 0 0 0.0003 0 0 0
Eisosomes 0.0001 0 0 0.0002 0.0002 0 0.0002 0 0 0.0004 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0036 0.001 0.0008 0.0018 0.0027 0.0004 0.0037 0.0004 0.0003 0.0008 0.0029 0.0004 0 0 0.0001 0 0 0
Endosome 0.0382 0.0121 0.0166 0.0042 0.0156 0.0176 0.0235 0.0002 0.0058 0.0045 0.0222 0.0023 0 0.0001 0.0003 0 0 0
Golgi 0.0067 0.0008 0.0032 0.004 0.0119 0.0045 0.0079 0 0.0055 0.0031 0.0085 0.0014 0 0 0 0 0 0
Lipid Particles 0.0157 0.0006 0.011 0.0038 0.0456 0.0017 0.0239 0 0.0029 0.006 0.0039 0.0029 0 0 0 0 0 0
Mitochondria 0.0079 0.001 0.0027 0.0033 0.0832 0.0116 0.0109 0.0002 0.0184 0.0039 0.0019 0.0017 0.0001 0.0001 0.0002 0.0001 0.0001 0.0001
None 0.0027 0.0003 0.0015 0.0013 0.0009 0.0003 0.002 0.0241 0.004 0.0122 0.0004 0.001 0 0 0.0031 0 0 0.0072
Nuclear Periphery 0.0154 0.0076 0.0039 0.0196 0.0226 0.0043 0.0235 0.0077 0.0079 0.0326 0.0324 0.0107 0.0006 0.0036 0.0029 0.0013 0.0004 0.0004
Nucleolus 0.0092 0.0079 0.0083 0.0082 0.0173 0.0156 0.0055 0.0116 0.0065 0.0051 0.0042 0.0105 0.0042 0.0038 0.0074 0.0053 0.0122 0.0093
Nucleus 0.7939 0.9345 0.9145 0.8996 0.7071 0.9109 0.8124 0.9446 0.9225 0.8655 0.8978 0.9551 0.9946 0.9911 0.9766 0.9922 0.9861 0.9795
Peroxisomes 0.0129 0.0002 0.0102 0.0006 0.0214 0.0008 0.0105 0 0.0032 0.0042 0.0007 0.0006 0 0 0 0 0 0
Punctate Nuclear 0.0168 0.0133 0.003 0.0022 0.0127 0.0015 0.0137 0.0008 0.0073 0.011 0.0022 0.0045 0.0002 0.0004 0.0034 0.0004 0.0002 0.0017
Vacuole 0.0087 0.0058 0.0014 0.0045 0.0171 0.0019 0.0038 0.0008 0.001 0.0008 0.0009 0.0007 0 0.0001 0.0001 0.0002 0.0001 0.0001
Vacuole Periphery 0.0028 0.0007 0.0006 0.0014 0.0058 0.0007 0.0028 0.0001 0.0005 0.002 0.0026 0.0005 0 0 0 0 0 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 8.6396 4.4133 4.9123 9.7579 11.2017 2.4671 8.3668 12.7999 8.2536 10.7614
Translational Efficiency 1.7462 2.9955 2.8916 1.5561 1.206 7.771 2.3259 1.5439 2.7385 1.9486

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1684 939 192 1216 2044 1343 1404 1215 3728 2282 1596 2431

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 734.91 865.83 1103.77 847.31 787.31 983.03 831.64 812.85 763.64 934.80 864.38 830.09
Standard Deviation 366.51 152.86 151.76 177.94 154.65 164.58 111.02 105.81 272.90 169.94 146.46 147.41
Intensity Change Log 2 — 0.236516 0.586800 0.205322 — 0.320304 0.079027 0.046057 — 0.280459 0.346462 0.125145

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000180 0.000569 0.001313 0.001714 0.000159 0.000448 0.000466 0.000663 0.000168 0.000498 0.000568 0.001188
Bud Neck 0.001781 0.008304 0.008352 0.020275 0.003207 0.009575 0.011427 0.011803 0.002563 0.009052 0.011057 0.016041
Bud Site 0.000882 0.001799 0.007163 0.026362 0.000571 0.001234 0.004557 0.015691 0.000711 0.001466 0.004870 0.021029
Cell Periphery 0.000378 0.000329 0.000080 0.000387 0.000261 0.000162 0.000158 0.000262 0.000314 0.000230 0.000149 0.000325
Cytoplasm 0.010326 0.003890 0.002144 0.073905 0.002261 0.005078 0.023533 0.071054 0.005904 0.004589 0.020960 0.072480
Cytoplasmic Foci 0.000458 0.001312 0.000082 0.004130 0.000173 0.001022 0.001049 0.002656 0.000302 0.001142 0.000932 0.003393
Eisosomes 0.000018 0.000025 0.000044 0.000066 0.000018 0.000022 0.000023 0.000030 0.000018 0.000023 0.000026 0.000048
Endoplasmic Reticulum 0.006354 0.008499 0.009214 0.008109 0.005745 0.007262 0.004684 0.006678 0.006020 0.007771 0.005229 0.007394
Endosome 0.000223 0.000726 0.000296 0.007068 0.000244 0.000512 0.001387 0.002840 0.000235 0.000600 0.001255 0.004955
Golgi 0.000036 0.000786 0.000085 0.008407 0.000044 0.000198 0.000091 0.001872 0.000041 0.000440 0.000090 0.005141
Lipid Particles 0.000084 0.000341 0.000238 0.000869 0.000112 0.000387 0.000470 0.001391 0.000099 0.000368 0.000442 0.001130
Mitochondria 0.000419 0.004128 0.001370 0.037308 0.001184 0.003063 0.001742 0.004442 0.000839 0.003501 0.001698 0.020882
Mitotic Spindle 0.000025 0.002079 0.000124 0.038072 0.000249 0.007337 0.004571 0.010026 0.000148 0.005173 0.004036 0.024055
None 0.022077 0.004158 0.001323 0.006326 0.004218 0.002342 0.006369 0.005467 0.012285 0.003089 0.005762 0.005896
Nuclear Periphery 0.000287 0.000451 0.000426 0.002592 0.000753 0.001265 0.000933 0.002322 0.000543 0.000930 0.000872 0.002457
Nuclear Periphery Foci 0.000182 0.000298 0.000241 0.000795 0.000209 0.000349 0.000273 0.000577 0.000197 0.000328 0.000270 0.000686
Nucleolus 0.005553 0.004807 0.003208 0.001442 0.003906 0.003840 0.002582 0.001216 0.004650 0.004238 0.002657 0.001329
Nucleus 0.949591* 0.955668* 0.959771* 0.731884* 0.975697* 0.953442* 0.927932* 0.840131* 0.963904* 0.954358* 0.931762* 0.785985*
Peroxisomes 0.000098 0.000606 0.000339 0.000718 0.000180 0.000585 0.000234 0.000491 0.000143 0.000594 0.000247 0.000604
Vacuole 0.000966 0.000969 0.004038 0.024433 0.000606 0.001530 0.006845 0.018548 0.000769 0.001299 0.006507 0.021492
Vacuole Periphery 0.000082 0.000256 0.000150 0.005138 0.000203 0.000347 0.000673 0.001841 0.000148 0.000310 0.000610 0.003490

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -5.81 -11.03 -6.45 -4.12 0.73 -5.89 -9.57 -5.92 -2.07 -1.55 -8.25 -13.17 -8.40 -4.68 -3.36
Bud Neck -5.58 -5.97 -10.39 -4.88 -4.66 -8.55 -8.15 -8.56 -1.89 -0.08 -10.02 -9.47 -13.31 -5.12 -2.50
Bud Site -1.83 -1.21 -6.70 -6.26 -2.06 -3.02 -5.21 -5.12 -4.72 -2.83 -3.14 -4.75 -8.43 -7.87 -4.99
Cell Periphery 0.53 5.43 2.04 0.83 -4.35 1.47 2.03 0.91 -0.52 -0.92 1.50 4.29 1.88 0.11 -2.45
Cytoplasm 3.65 4.61 -13.62 -15.67 -16.09 -3.04 -10.61 -17.06 -15.74 -10.15 1.10 -8.01 -21.84 -22.16 -14.18
Cytoplasmic Foci -0.98 3.19 -4.04 -1.19 -5.09 -1.74 -2.73 -4.66 -1.54 -2.11 -1.87 -2.15 -6.16 -1.97 -3.32
Eisosomes -2.76 -8.17 -6.78 -5.55 -1.54 -2.08 -4.37 -8.65 -7.21 -5.68 -3.36 -7.23 -9.54 -7.83 -5.98
Endoplasmic Reticulum -3.01 -3.62 -5.60 -2.66 -0.01 -4.13 0.21 -5.79 -2.65 -5.97 -4.82 -0.60 -8.13 -4.01 -7.52
Endosome -2.44 -2.08 -4.48 -3.68 -4.29 -3.37 -2.56 -3.72 -3.07 -1.28 -3.82 -2.63 -5.77 -4.81 -3.03
Golgi -1.80 -4.45 -3.13 -2.28 -3.07 -1.96 -1.86 -1.75 -1.44 -1.66 -2.26 -2.61 -3.58 -2.80 -3.49
Lipid Particles -1.63 -4.90 -7.85 -3.22 -5.56 -2.30 -3.81 -3.25 -2.23 -2.00 -2.82 -4.27 -5.53 -3.47 -3.03
Mitochondria -2.31 -3.78 -5.35 -3.72 -4.96 -1.81 -1.25 -2.52 -0.25 -2.05 -2.97 -2.97 -5.78 -3.71 -5.22
Mitotic Spindle -2.06 -2.22 -7.69 -6.67 -7.65 -3.83 -3.81 -4.87 -0.64 -2.11 -4.33 -3.90 -9.02 -5.48 -6.30
None 7.00 8.57 6.36 -1.95 -6.74 1.87 -1.24 -0.19 -2.93 1.35 7.16 5.11 5.49 -3.65 -0.22
Nuclear Periphery -3.39 -3.71 -19.27 -18.14 -17.51 -1.83 -2.55 -9.17 -6.18 -8.61 -2.34 -5.22 -17.73 -12.92 -15.33
Nuclear Periphery Foci -1.32 -0.83 -4.73 -3.27 -2.77 -1.22 -1.57 -3.89 -2.47 -2.29 -1.73 -1.90 -6.02 -3.94 -3.23
Nucleolus 1.11 3.66 7.93 5.58 3.69 -0.01 1.45 5.88 5.22 2.98 0.79 2.98 9.54 7.38 2.97
Nucleus -0.97 -0.91 19.86 19.83 17.90 5.97 11.27 20.46 15.30 11.17 3.41 8.59 28.38 24.85 18.82
Peroxisomes -2.50 -7.78 -3.47 0.83 -0.46 -1.84 -0.41 -1.18 1.12 -1.01 -2.91 -1.75 -2.92 1.35 -1.79
Vacuole -0.27 -4.90 -13.67 -13.66 -11.94 -5.41 -7.62 -13.04 -12.53 -9.06 -4.33 -7.97 -18.83 -18.45 -13.65
Vacuole Periphery -1.62 -3.48 -3.24 -2.91 -3.10 -1.33 -1.63 -1.45 -1.19 -0.76 -2.15 -1.97 -3.38 -2.97 -2.33
Standard name
Human Ortholog
Description Component of the U2 snRNP; required for the first catalytic step of splicing and for spliceosomal assembly; interacts with Rds3p and is required for Mer1p-activated splicing; diploid mutants have a specific defect in MATa1 pre-mRNA splicing which leads to haploid gene expression in diploids
Localization
Cell Percentages nucleus (100%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Ist3

Ist3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ist3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available