Standard name | |
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Human Ortholog | |
Description | Component of the U2 snRNP; required for the first catalytic step of splicing and for spliceosomal assembly; interacts with Rds3p and is required for Mer1p-activated splicing; diploid mutants have a specific defect in MATa1 pre-mRNA splicing which leads to haploid gene expression in diploids |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 1 | 1 | 2 | – | 5 | 2 | 11 | 6 | 7 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 3 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 1 | 1 | 9 | – | 24 | 12 | 26 | 27 | 27 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 1 | 5 | 14 | 24 | 20 | – | 27 | 20 | 15 | 22 | 15 | 14 | 42 | 53 | 27 | 28 | 18 | 0 | 1 | 0 | 1 | 1 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 5 | 9 | 4 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 7 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 3 | 2 |
Golgi | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
Mitochondria | 8 | 2 | 25 | 95 | 72 | 176 | – | 297 | 259 | 360 | 347 | 418 | 5 | 2 | 4 | 5 | 6 | 3 | 0 | 0 | 0 | 0 | 2 | 2 |
Nucleus | 114 | 106 | 117 | 299 | 494 | 421 | – | 664 | 355 | 568 | 448 | 582 | 180 | 277 | 293 | 182 | 215 | 117 | 117 | 107 | 120 | 51 | 239 | 228 |
Nuclear Periphery | 0 | 0 | 0 | 1 | 4 | 11 | – | 17 | 17 | 17 | 12 | 14 | 0 | 10 | 12 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 1 | 0 | 1 | 4 | 5 | 23 | – | 50 | 27 | 45 | 30 | 44 | 0 | 2 | 6 | 1 | 5 | 1 | 0 | 0 | 0 | 0 | 3 | 14 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | – | 6 | 0 | 2 | 2 | 1 | 0 | 1 | 3 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 3 |
Vac/Vac Membrane | 5 | 3 | 1 | 6 | 10 | 6 | – | 15 | 2 | 3 | 0 | 6 | 3 | 5 | 11 | 19 | 15 | 15 | 3 | 2 | 3 | 3 | 39 | 74 |
Unique Cell Count | 122 | 112 | 128 | 330 | 548 | 484 | 821 | 452 | 684 | 573 | 715 | 196 | 325 | 367 | 223 | 262 | 154 | 122 | 115 | 130 | 59 | 298 | 337 | |
Labelled Cell Count | 128 | 112 | 149 | 421 | 613 | 669 | 1105 | 694 | 1048 | 895 | 1114 | 203 | 340 | 392 | 244 | 279 | 162 | 122 | 115 | 130 | 59 | 298 | 337 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.4 | 9.0 | 5.7 | 5.3 | 5.6 | 4.6 | – | 4.4 | 3.8 | 3.9 | 3.9 | 3.6 | 7.3 | 7.5 | 7.7 | 10.1 | 10.1 | 10.5 | 6.3 | 7.7 | 7.8 |
Std Deviation (1e-4) | 1.4 | 1.9 | 1.1 | 1.2 | 1.3 | 1.3 | – | 1.4 | 1.5 | 1.1 | 1.8 | 1.1 | 1.7 | 1.8 | 2.5 | 2.3 | 2.4 | 2.6 | 1.1 | 1.6 | 1.7 |
Intensity Change (Log2) | – | – | – | -0.1 | -0.02 | -0.31 | – | -0.36 | -0.55 | -0.54 | -0.55 | -0.66 | 0.36 | 0.41 | 0.44 | 0.84 | 0.84 | 0.9 | 0.16 | 0.45 | 0.46 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.2 | 0.2 | 0.1 | – | 0 | 0.3 | 0 | 0 | 0 | 1.2 | 2.8 | 3.2 | 2.6 | 2.3 | 2.4 |
Endoplasmic Reticulum | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 2.0 | -1.9 | 0 | – | 0 | 0 | 0 | 0 | 0 | -5.2 | -7.7 | -7.6 | -5.6 | -5.9 | -4.9 |
Nucleus | -0.3 | -0.4 | -1.4 | – | -2.9 | -3.3 | -2.4 | -3.4 | -2.8 | 0.1 | -1.8 | -3.0 | -2.5 | -2.4 | -3.4 |
Nuclear Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.0 | 2.3 | 3.2 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.1452 | 2.6638 | 2.2353 | 1.8184 | 1.8413 | 2.1279 | 1.223 | 2.0503 | 1.2208 | 1.322 | 1.4531 | 1.4754 | 2.8338 | 4.1622 | 3.6177 | 3.1753 | 3.0343 | 3.3118 |
Actin | 0.0152 | 0.0001 | 0.0029 | 0.0327 | 0.0122 | 0.0066 | 0.0218 | 0 | 0.0017 | 0.0354 | 0.0008 | 0.0009 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0.0012 | 0.0003 | 0.0001 | 0.0007 | 0.001 | 0.0003 | 0.0004 | 0 | 0.0001 | 0.0007 | 0.0003 | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0.0034 | 0.0008 | 0.0027 | 0.0018 | 0.0017 | 0.0014 | 0.0013 | 0.0027 | 0.0014 | 0.0003 | 0.0003 | 0.0009 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0007 | 0.0004 |
Bud Periphery | 0.0023 | 0.0003 | 0.0002 | 0.0015 | 0.0036 | 0.0007 | 0.0009 | 0.0001 | 0.0002 | 0.0007 | 0.0002 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0.0057 | 0.0027 | 0.0013 | 0.0017 | 0.001 | 0.0004 | 0.0023 | 0.0001 | 0.0004 | 0.0042 | 0.0001 | 0.0001 | 0 | 0 | 0.0002 | 0 | 0 | 0 |
Cell Periphery | 0.0004 | 0.0001 | 0.0001 | 0.0004 | 0.0003 | 0.0001 | 0.0002 | 0.0003 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.0151 | 0.0061 | 0.0077 | 0.0056 | 0.0042 | 0.0123 | 0.0055 | 0.0061 | 0.0058 | 0.0013 | 0.0112 | 0.0046 | 0.0002 | 0.0006 | 0.0052 | 0.0002 | 0.0001 | 0.001 |
Cytoplasmic Foci | 0.0221 | 0.0038 | 0.0072 | 0.0009 | 0.0118 | 0.0065 | 0.0233 | 0.0001 | 0.0044 | 0.0053 | 0.0065 | 0.0009 | 0 | 0 | 0.0003 | 0 | 0 | 0 |
Eisosomes | 0.0001 | 0 | 0 | 0.0002 | 0.0002 | 0 | 0.0002 | 0 | 0 | 0.0004 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0036 | 0.001 | 0.0008 | 0.0018 | 0.0027 | 0.0004 | 0.0037 | 0.0004 | 0.0003 | 0.0008 | 0.0029 | 0.0004 | 0 | 0 | 0.0001 | 0 | 0 | 0 |
Endosome | 0.0382 | 0.0121 | 0.0166 | 0.0042 | 0.0156 | 0.0176 | 0.0235 | 0.0002 | 0.0058 | 0.0045 | 0.0222 | 0.0023 | 0 | 0.0001 | 0.0003 | 0 | 0 | 0 |
Golgi | 0.0067 | 0.0008 | 0.0032 | 0.004 | 0.0119 | 0.0045 | 0.0079 | 0 | 0.0055 | 0.0031 | 0.0085 | 0.0014 | 0 | 0 | 0 | 0 | 0 | 0 |
Lipid Particles | 0.0157 | 0.0006 | 0.011 | 0.0038 | 0.0456 | 0.0017 | 0.0239 | 0 | 0.0029 | 0.006 | 0.0039 | 0.0029 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0.0079 | 0.001 | 0.0027 | 0.0033 | 0.0832 | 0.0116 | 0.0109 | 0.0002 | 0.0184 | 0.0039 | 0.0019 | 0.0017 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 |
None | 0.0027 | 0.0003 | 0.0015 | 0.0013 | 0.0009 | 0.0003 | 0.002 | 0.0241 | 0.004 | 0.0122 | 0.0004 | 0.001 | 0 | 0 | 0.0031 | 0 | 0 | 0.0072 |
Nuclear Periphery | 0.0154 | 0.0076 | 0.0039 | 0.0196 | 0.0226 | 0.0043 | 0.0235 | 0.0077 | 0.0079 | 0.0326 | 0.0324 | 0.0107 | 0.0006 | 0.0036 | 0.0029 | 0.0013 | 0.0004 | 0.0004 |
Nucleolus | 0.0092 | 0.0079 | 0.0083 | 0.0082 | 0.0173 | 0.0156 | 0.0055 | 0.0116 | 0.0065 | 0.0051 | 0.0042 | 0.0105 | 0.0042 | 0.0038 | 0.0074 | 0.0053 | 0.0122 | 0.0093 |
Nucleus | 0.7939 | 0.9345 | 0.9145 | 0.8996 | 0.7071 | 0.9109 | 0.8124 | 0.9446 | 0.9225 | 0.8655 | 0.8978 | 0.9551 | 0.9946 | 0.9911 | 0.9766 | 0.9922 | 0.9861 | 0.9795 |
Peroxisomes | 0.0129 | 0.0002 | 0.0102 | 0.0006 | 0.0214 | 0.0008 | 0.0105 | 0 | 0.0032 | 0.0042 | 0.0007 | 0.0006 | 0 | 0 | 0 | 0 | 0 | 0 |
Punctate Nuclear | 0.0168 | 0.0133 | 0.003 | 0.0022 | 0.0127 | 0.0015 | 0.0137 | 0.0008 | 0.0073 | 0.011 | 0.0022 | 0.0045 | 0.0002 | 0.0004 | 0.0034 | 0.0004 | 0.0002 | 0.0017 |
Vacuole | 0.0087 | 0.0058 | 0.0014 | 0.0045 | 0.0171 | 0.0019 | 0.0038 | 0.0008 | 0.001 | 0.0008 | 0.0009 | 0.0007 | 0 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0001 |
Vacuole Periphery | 0.0028 | 0.0007 | 0.0006 | 0.0014 | 0.0058 | 0.0007 | 0.0028 | 0.0001 | 0.0005 | 0.002 | 0.0026 | 0.0005 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 8.6396 | 4.4133 | 4.9123 | 9.7579 | 11.2017 | 2.4671 | 8.3668 | 12.7999 | 8.2536 | 10.7614 |
Translational Efficiency | 1.7462 | 2.9955 | 2.8916 | 1.5561 | 1.206 | 7.771 | 2.3259 | 1.5439 | 2.7385 | 1.9486 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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